1、 Introduction:

    Wuhan Miaoling Biotechnology Co., Ltd. is located in Wuhan Optics Valley Biotech City. It is a high-tech enterprise specializing in the research, development, production, and sales of plasmid vectors and related products. The company mainly serves major universities, research institutes, hospitals, and related biopharmaceutical enterprises. The company has a professional R&D team and a team of nearly 100 molecular experiment operators. More than 90% of the personnel have a bachelor's degree or above. The total laboratory area is nearly 1500 square meters, with molecular laboratories, R&D laboratories, cell virus laboratories, and large-scale plasmid storage libraries. Based on the principle of self-produced and self sold, product first, we have developed a corporate culture that we have been striving for, hoping to create more possibilities for scientific research activities with Miaoling plasmid products!

Corporate Vision: Committed to providing excellent plasmid products for all researchers

Corporate mission: To become the world's largest plasmid supplier

Corporate values: Innovation, professionalism, inclusiveness, sustainability

2、 Main business:

Vector construction, plasmid extraction, plasmid preservation, protein expression, virus packaging, cell culture

3、 Product ordering process:

Spot plasmid:

Search for the required plasmid on the official website - Consult the person in charge (contact information is as follows) - Confirm plasmid information, price, and delivery time - Order payment - Shipment acceptance - Issue invoice

Constructing plasmids:

Consultation with the person in charge to place an order (contact information of the person in charge is as follows) - Confirm plasmid information, price, and delivery time - Order payment - Shipment acceptance - Issue invoice

4、 Contact information:

Contact email:publicemail-1@miaolingbio.com

WeCom：

5、 Product operation instructions:

① Dry powder plasmid (stored at negative 20 degrees, shelf life of 90 days)

1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1 minute, and then add 20 μ l of sterile water to dissolve the plasmid;

2. Thaw the corresponding state on an ice box, take 2 μ l of plasmid and add it to 100 μ l of state, and ice bath for 30 minutes;

3. Heat shock at 42 degrees for 60 seconds, ice bath for 2 minutes, add 900 μ l of anti LB medium, shake at 180rpm for 45 minutes;

4. Centrifuge at 6000rpm for 3 minutes, retain 100 μ l of supernatant for resuspension, and then coat onto the corresponding resistant LB plate;

5. After overnight inverted cultivation, if there are too many colonies, dilute the plasmid for transformation. If there are no colonies, take 10ul of plasmid for transformation;

6. Select single colonies and shake overnight in LB liquid medium corresponding to resistance, and extract plasmids as needed.

② Filter paper plasmid (stored at negative 20 degrees, shelf life of 90 days)

1. After receiving the goods, cut off the circled part of the filter paper and put it into an EP tube;

2. Soak the filter paper in 100 μ l of sterile water for 5 minutes;

3. Transfer 10 μ l of plasmid into 100 μ l of corresponding competent state and centrifuge for full coating.

6、 Company Address:

Building C1, Optics Valley Biotech City, No. 666 Gaoxin Avenue, Hongshan District, Wuhan City