名称: | pCAG-enAsCas12a-HF1(E174R-N282A-S542R-K548R)-NLS(nuc)-3xHA(AAS1815) |
---|---|
别称: | Plasmid#107942 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CAG |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | pcaggs-F:GTTCGGCTTCTGGCGTGT |
反向引物: | pcaggs-R:TATGTCCTTCCGAGTGAGAG |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GGTCGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAC
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGGTCGA
GGTGAGCCCC
ACGTTCTGCT
TCACTCTCCC
CATCTCCCCC
CCCTCCCCAC
CCCCAATTTT
GTATTTATTT
ATTTTTTAAT
TATTTTGTGC
AGCGATGGGG
GCGGGGGGGG
GGGGGGCGCG
CGCCAGGCGG
GGCGGGGCGG
GGCGAGGGGC
GGGGCGGGGC
GAGGCGGAGA
GGTGCGGCGG
CAGCCAATCA
GAGCGGCGCG
CTCCGAAAGT
TTCCTTTTAT
GGCGAGGCGG
CGGCGGCGGC
GGCCCTATAA
AAAGCGAAGC
GCGCGGCGGG
CGGGAGTCGC
TGCGTTGCCT
TCGCCCCGTG
CCCCGCTCCG
CGCCGCCTCG
CGCCGCCCGC
CCCGGCTCTG
ACTGACCGCG
TTACTCCCAC
AGGTGAGCGG
GCGGGACGGC
CCTTCTCCTC
CGGGCTGTAA
TTAGCGCTTG
GTTTAATGAC
GGCTCGTTTC
TTTTCTGTGG
CTGCGTGAAA
GCCTTAAAGG
GCTCCGGGAG
GGCCCTTTGT
GCGGGGGGGA
GCGGCTCGGG
GGGTGCGTGC
GTGTGTGTGT
GCGTGGGGAG
CGCCGCGTGC
GGCCCGCGCT
GCCCGGCGGC
TGTGAGCGCT
GCGGGCGCGG
CGCGGGGCTT
TGTGCGCTCC
GCGTGTGCGC
GAGGGGAGCG
CGGCCGGGGG
CGGTGCCCCG
CGGTGCGGGG
GGGCTGCGAG
GGGAACAAAG
GCTGCGTGCG
GGGTGTGTGC
GTGGGGGGGT
GAGCAGGGGG
TGTGGGCGCG
GCGGTCGGGC
TGTAACCCCC
CCCTGCACCC
CCCTCCCCGA
GTTGCTGAGC
ACGGCCCGGC
TTCGGGTGCG
GGGCTCCGTG
CGGGGCGTGG
CGCGGGGCTC
GCCGTGCCGG
GCGGGGGGTG
GCGGCAGGTG
GGGGTGCCGG
GCGGGGCGGG
GCCGCCTCGG
GCCGGGGAGG
GCTCGGGGGA
GGGGCGCGGC
GGCCCCGGAG
CGCCGGCGGC
TGTCGAGGCG
CGGCGAGCCG
CAGCCATTGC
CTTTTATGGT
AATCGTGCGA
GAGGGCGCAG
GGACTTCCTT
TGTCCCAAAT
CTGGCGGAGC
CGAAATCTGG
GAGGCGCCGC
CGCACCCCCT
CTAGCGGGCG
CGGGCGAAGC
GGTGCGGCGC
CGGCAGGAAG
GAAATGGGCG
GGGAGGGCCT
TCGTGCGTCG
CCGCGCCGCC
GTCCCCTTCT
CCATCTCCAG
CCTCGGGGCT
GCCGCAGGGG
GACGGCTGCC
TTCGGGGGGG
ACGGGGCAGG
GCGGGGTTCG
GCTTCTGGCG
TGTGACCGGC
GGCTCTAGAG
CCTCTGCTAA
CCATGTTCAT
GCCTTCTTCT
TTTTCCTACA
GCTCCTGGGC
AACGTGCTGG
TTATTGTGCT
GTCTCATCAT
TTTGGCAAAG
AATTCTGCAG
TCGACGGTAC
CGCGGGCCCG
GGATCCACCG
GTCGCCACCA
TGACACAGTT
CGAGGGCTTT
ACCAACCTGT
ATCAGGTGAG
CAAGACACTG
CGGTTTGAGC
TGATCCCACA
GGGCAAGACC
CTGAAGCACA
TCCAGGAGCA
GGGCTTCATC
GAGGAGGACA
AGGCCCGCAA
TGATCACTAC
AAGGAGCTGA
AGCCCATCAT
CGATCGGATC
TACAAGACCT
ATGCCGACCA
GTGCCTGCAG
CTGGTGCAGC
TGGATTGGGA
GAACCTGAGC
GCCGCCATCG
ACTCCTATAG
AAAGGAGAAA
ACCGAGGAGA
CAAGGAACGC
CCTGATCGAG
GAGCAGGCCA
CATATCGCAA
TGCCATCCAC
GACTACTTCA
TCGGCCGGAC
AGACAACCTG
ACCGATGCCA
TCAATAAGAG
ACACGCCGAG
ATCTACAAGG
GCCTGTTCAA
GGCCGAGCTG
TTTAATGGCA
AGGTGCTGAA
GCAGCTGGGC
ACCGTGACCA
CAACCGAGCA
CGAGAACGCC
CTGCTGCGGA
GCTTCGACAA
GTTTACAACC
TACTTCTCCG
GCTTTTATAG
AAACAGGAAG
AACGTGTTCA
GCGCCGAGGA
TATCAGCACA
GCCATCCCAC
ACCGCATCGT
GCAGGACAAC
TTCCCCAAGT
TTAAGGAGAA
TTGTCACATC
TTCACACGCC
TGATCACCGC
CGTGCCCAGC
CTGCGGGAGC
ACTTTGAGAA
CGTGAAGAAG
GCCATCGGCA
TCTTCGTGAG
CACCTCCATC
GAGGAGGTGT
TTTCCTTCCC
TTTTTATAAC
CAGCTGCTGA
CACAGACCCA
GATCGACCTG
TATAACCAGC
TGCTGGGAGG
AATCTCTCGG
GAGGCAGGCA
CCGAGAAGAT
CAAGGGCCTG
AACGAGGTGC
TGGCCCTGGC
CATCCAGAAG
AATGATGAGA
CAGCCCACAT
CATCGCCTCC
CTGCCACACA
GATTCATCCC
CCTGTTTAAG
CAGATCCTGT
CCGATAGGAA
CACCCTGTCT
TTCATCCTGG
AGGAGTTTAA
GAGCGACGAG
GAAGTGATCC
AGTCCTTCTG
CAAGTACAAG
ACACTGCTGA
GAAACGAGAA
CGTGCTGGAG
ACAGCCGAGG
CCCTGTTTAA
CGAGCTGAAC
AGCATCGACC
TGACACACAT
CTTCATCAGC
CACAAGAAGC
TGGAGACAAT
CAGCAGCGCC
CTGTGCGACC
ACTGGGATAC
ACTGAGGAAT
GCCCTGTATG
AGCGGAGAAT
CTCCGAGCTG
ACAGGCAAGA
TCACCAAGTC
TGCCAAGGAG
AAGGTGCAGC
GCAGCCTGAA
GCACGAGGAT
ATCAACCTGC
AGGAGATCAT
CTCTGCCGCA
GGCAAGGAGC
TGAGCGAGGC
CTTCAAGCAG
AAAACCAGCG
AGATCCTGTC
CCACGCACAC
GCCGCCCTGG
ATCAGCCACT
GCCTACAACC
CTGAAGAAGC
AGGAGGAGAA
GGAGATCCTG
AAGTCTCAGC
TGGACAGCCT
GCTGGGCCTG
TACCACCTGC
TGGACTGGTT
TGCCGTGGAT
GAGTCCAACG
AGGTGGACCC
CGAGTTCTCT
GCCCGGCTGA
CCGGCATCAA
GCTGGAGATG
GAGCCTTCTC
TGAGCTTCTA
CAACAAGGCC
AGAAATTATG
CCACCAAGAA
GCCCTACTCC
GTGGAGAAGT
TCAAGCTGAA
CTTTCAGATG
CCTACACTGG
CCAGAGGCTG
GGACGTGAAT
AGAGAGAAGA
ACAATGGCGC
CATCCTGTTT
GTGAAGAACG
GCCTGTACTA
TCTGGGCATC
ATGCCAAAGC
AGAAGGGCAG
GTATAAGGCC
CTGAGCTTCG
AGCCCACAGA
GAAAACCAGC
GAGGGCTTTG
ATAAGATGTA
CTATGACTAC
TTCCCTGATG
CCGCCAAGAT
GATCCCAAAG
TGCAGCACCC
AGCTGAAGGC
CGTGACAGCC
CACTTTCAGA
CCCACACAAC
CCCCATCCTG
CTGTCCAACA
ATTTCATCGA
GCCTCTGGAG
ATCACAAAGG
AGATCTACGA
CCTGAACAAT
CCTGAGAAGG
AGCCAAAGAA
GTTTCAGACA
GCCTACGCCA
AGAAAACCGG
CGACCAGAAG
GGCTACAGAG
AGGCCCTGTG
CAAGTGGATC
GACTTCACAA
GGGATTTTCT
GTCCAAGTAT
ACCAAGACAA
CCTCTATCGA
TCTGTCTAGC
CTGCGGCCAT
CCTCTCAGTA
TAAGGACCTG
GGCGAGTACT
ATGCCGAGCT
GAATCCCCTG
CTGTACCACA
TCAGCTTCCA
GAGAATCGCC
GAGAAGGAGA
TCATGGATGC
CGTGGAGACA
GGCAAGCTGT
ACCTGTTCCA
GATCTATAAC
AAGGACTTTG
CCAAGGGCCA
CCACGGCAAG
CCTAATCTGC
ACACACTGTA
TTGGACCGGC
CTGTTTTCTC
CAGAGAACCT
GGCCAAGACA
AGCATCAAGC
TGAATGGCCA
GGCCGAGCTG
TTCTACCGCC
CTAAGTCCAG
GATGAAGAGG
ATGGCACACC
GGCTGGGAGA
GAAGATGCTG
AACAAGAAGC
TGAAGGATCA
GAAAACCCCA
ATCCCCGACA
CCCTGTACCA
GGAGCTGTAC
GACTATGTGA
ATCACAGACT
GTCCCACGAC
CTGTCTGATG
AGGCCAGGGC
CCTGCTGCCC
AACGTGATCA
CCAAGGAGGT
GTCTCACGAG
ATCATCAAGG
ATAGGCGCTT
TACCAGCGAC
AAGTTCTTTT
TCCACGTGCC
TATCACACTG
AACTATCAGG
CCGCCAATTC
CCCATCTAAG
TTCAACCAGA
GGGTGAATGC
CTACCTGAAG
GAGCACCCCG
AGACACCTAT
CATCGGCATC
GATCGGGGCG
AGAGAAACCT
GATCTATATC
ACAGTGATCG
ACTCCACCGG
CAAGATCCTG
GAGCAGCGGA
GCCTGAACAC
CATCCAGCAG
TTTGATTACC
AGAAGAAGCT
GGACAACAGG
GAGAAGGAGA
GGGTGGCAGC
AAGGCAGGCC
TGGTCTGTGG
TGGGCACAAT
CAAGGATCTG
AAGCAGGGCT
ATCTGAGCCA
GGTCATCCAC
GAGATCGTGG
ACCTGATGAT
CCACTACCAG
GCCGTGGTGG
TGCTGGAGAA
CCTGAATTTC
GGCTTTAAGA
GCAAGAGGAC
CGGCATCGCC
GAGAAGGCCG
TGTACCAGCA
GTTCGAGAAG
ATGCTGATCG
ATAAGCTGAA
TTGCCTGGTG
CTGAAGGACT
ATCCAGCAGA
GAAAGTGGGA
GGCGTGCTGA
ACCCATACCA
GCTGACAGAC
CAGTTCACCT
CCTTTGCCAA
GATGGGCACC
CAGTCTGGCT
TCCTGTTTTA
CGTGCCTGCC
CCATATACAT
CTAAGATCGA
TCCCCTGACC
GGCTTCGTGG
ACCCCTTCGT
GTGGAAAACC
ATCAAGAATC
ACGAGAGCCG
CAAGCACTTC
CTGGAGGGCT
TCGACTTTCT
GCACTACGAC
GTGAAAACCG
GCGACTTCAT
CCTGCACTTT
AAGATGAACA
GAAATCTGTC
CTTCCAGAGG
GGCCTGCCCG
GCTTTATGCC
TGCATGGGAT
ATCGTGTTCG
AGAAGAACGA
GACACAGTTT
GACGCCAAGG
GCACCCCTTT
CATCGCCGGC
AAGAGAATCG
TGCCAGTGAT
CGAGAATCAC
AGATTCACCG
GCAGATACCG
GGACCTGTAT
CCTGCCAACG
AGCTGATCGC
CCTGCTGGAG
GAGAAGGGCA
TCGTGTTCAG
GGATGGCTCC
AACATCCTGC
CAAAGCTGCT
GGAGAATGAC
GATTCTCACG
CCATCGACAC
CATGGTGGCC
CTGATCCGCA
GCGTGCTGCA
GATGCGGAAC
TCCAATGCCG
CCACAGGCGA
GGACTATATC
AACAGCCCCG
TGCGCGATCT
GAATGGCGTG
TGCTTCGACT
CCCGGTTTCA
GAACCCAGAG
TGGCCCATGG
ACGCCGATGC
CAATGGCGCC
TACCACATCG
CCCTGAAGGG
CCAGCTGCTG
CTGAATCACC
TGAAGGAGAG
CAAGGATCTG
AAGCTGCAGA
ACGGCATCTC
CAATCAGGAC
TGGCTGGCCT
ACATCCAGGA
GCTGCGCAAC
AAAAGGCCGG
CGGCCACGAA
AAAGGCCGGC
CAGGCAAAAA
AGAAAAAGGG
ATCCTACCCA
TACGATGTTC
CAGATTACGC
TTATCCCTAC
GACGTGCCTG
ATTATGCATA
CCCATATGAT
GTCCCCGACT
ATGCCTAAAG
CGGCCGCACT
CCTCAGGTGC
AGGCTGCCTA
TCAGAAGGTG
GTGGCTGGTG
TGGCCAATGC
CCTGGCTCAC
AAATACCACT
GAGATCTTTT
TCCCTCTGCC
AAAAATTATG
GGGACATCAT
GAAGCCCCTT
GAGCATCTGA
CTTCTGGCTA
ATAAAGGAAA
TTTATTTTCA
TTGCAATAGT
GTGTTGGAAT
TTTTTGTGTC
TCTCACTCGG
AAGGACATAT
GGGAGGGCAA
ATCATTTAAA
ACATCAGAAT
GAGTATTTGG
TTTAGAGTTT
GGCAACATAT
GCCCATATGC
TGGCTGCCAT
GAACAAAGGT
TGGCTATAAA
GAGGTCATCA
GTATATGAAA
CAGCCCCCTG
CTGTCCATTC
CTTATTCCAT
AGAAAAGCCT
TGACTTGAGG
TTAGATTTTT
TTTATATTTT
GTTTTGTGTT
ATTTTTTTCT
TTAACATCCC
TAAAATTTTC
CTTACATGTT
TTACTAGCCA
GATTTTTCCT
CCTCTCCTGA
CTACTCCCAG
TCATAGCTGT
CCCTCTTCTC
TTATGGAGAT
CCCTCGACCT
GCAGCCCAAG
CTTGGCGTAA
TCATGGTCAT
AGCTGTTTCC
TGTGTGAAAT
TGTTATCCGC
TCACAATTCC
ACACAACATA
CGAGCCGGAA
GCATAAAGTG
TAAAGCCTGG
GGTGCCTAAT
GAGTGAGCTA
ACTCACATTA
ATTGCGTTGC
GCTCACTGCC
CGCTTTCCAG
TCGGGAAACC
TGTCGTGCCA
GCGGATCCGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAAAGCTA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GATCCGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTG