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pAAV-RSV-SpCas9

产品货号:P8415

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pAAV-RSV-SpCas9
别称: Plasmid#85450
基因长:
质粒宿主: 哺乳细胞,腺相关病毒
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子:
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CCTGCAGGCA

GCTGCGCGCT

CGCTCGCTCA

CTGAGGCCGC

CCGGGCAAAG

CCCGGGCGTC

61

GGGCGACCTT

TGGTCGCCCG

GCCTCAGTGA

GCGAGCGAGC

GCGCAGAGAG

GGAGTGGCCA

121

ACTCCATCAC

TAGGGGTTCC

TGCGGCCTCT

AGAAATGTAG

TCTTATGCAA

TACTCTTGTA

181

GTCTTGCAAC

ATGGTAACGA

TGAGTTAGCA

ACATGCCTTA

CAAGGAGAGA

AAAAGCACCG

241

TGCATGCCGA

TTGGTGGAAG

TAAGGTGGTA

CGATCGTGCC

TTATTAGGAA

GGCAACAGAC

301

GGGTCTGACA

TGGATTGGAC

GAACCACTGA

ATTGCCGCAT

TGCAGAGATA

TTGTATTTAA

361

GTGCCTAGCT

CGATACATAA

AACCGGTGCC

ACCATGTACC

CATACGATGT

TCCAGATTAC

421

GCTTCGCCGA

AGAAAAAGCG

CAAGGTCGAA

GCGTCCGACA

AGAAGTACAG

CATCGGCCTG

481

GACATCGGCA

CCAACTCTGT

GGGCTGGGCC

GTGATCACCG

ACGAGTACAA

GGTGCCCAGC

541

AAGAAATTCA

AGGTGCTGGG

CAACACCGAC

CGGCACAGCA

TCAAGAAGAA

CCTGATCGGA

601

GCCCTGCTGT

TCGACAGCGG

CGAAACAGCC

GAGGCCACCC

GGCTGAAGAG

AACCGCCAGA

661

AGAAGATACA

CCAGACGGAA

GAACCGGATC

TGCTATCTGC

AAGAGATCTT

CAGCAACGAG

721

ATGGCCAAGG

TGGACGACAG

CTTCTTCCAC

AGACTGGAAG

AGTCCTTCCT

GGTGGAAGAG

781

GATAAGAAGC

ACGAGCGGCA

CCCCATCTTC

GGCAACATCG

TGGACGAGGT

GGCCTACCAC

841

GAGAAGTACC

CCACCATCTA

CCACCTGAGA

AAGAAACTGG

TGGACAGCAC

CGACAAGGCC

901

GACCTGCGGC

TGATCTATCT

GGCCCTGGCC

CACATGATCA

AGTTCCGGGG

CCACTTCCTG

961

ATCGAGGGCG

ACCTGAACCC

CGACAACAGC

GACGTGGACA

AGCTGTTCAT

CCAGCTGGTG

1021

CAGACCTACA

ACCAGCTGTT

CGAGGAAAAC

CCCATCAACG

CCAGCGGCGT

GGACGCCAAG

1081

GCCATCCTGT

CTGCCAGACT

GAGCAAGAGC

AGACGGCTGG

AAAATCTGAT

CGCCCAGCTG

1141

CCCGGCGAGA

AGAAGAATGG

CCTGTTCGGC

AACCTGATTG

CCCTGAGCCT

GGGCCTGACC

1201

CCCAACTTCA

AGAGCAACTT

CGACCTGGCC

GAGGATGCCA

AACTGCAGCT

GAGCAAGGAC

1261

ACCTACGACG

ACGACCTGGA

CAACCTGCTG

GCCCAGATCG

GCGACCAGTA

CGCCGACCTG

1321

TTTCTGGCCG

CCAAGAACCT

GTCCGACGCC

ATCCTGCTGA

GCGACATCCT

GAGAGTGAAC

1381

ACCGAGATCA

CCAAGGCCCC

CCTGAGCGCC

TCTATGATCA

AGAGATACGA

CGAGCACCAC

1441

CAGGACCTGA

CCCTGCTGAA

AGCTCTCGTG

CGGCAGCAGC

TGCCTGAGAA

GTACAAAGAG

1501

ATTTTCTTCG

ACCAGAGCAA

GAACGGCTAC

GCCGGCTACA

TTGACGGCGG

AGCCAGCCAG

1561

GAAGAGTTCT

ACAAGTTCAT

CAAGCCCATC

CTGGAAAAGA

TGGACGGCAC

CGAGGAACTG

1621

CTCGTGAAGC

TGAACAGAGA

GGACCTGCTG

CGGAAGCAGC

GGACCTTCGA

CAACGGCAGC

1681

ATCCCCCACC

AGATCCACCT

GGGAGAGCTG

CACGCCATTC

TGCGGCGGCA

GGAAGATTTT

1741

TACCCATTCC

TGAAGGACAA

CCGGGAAAAG

ATCGAGAAGA

TCCTGACCTT

CCGCATCCCC

1801

TACTACGTGG

GCCCTCTGGC

CAGGGGAAAC

AGCAGATTCG

CCTGGATGAC

CAGAAAGAGC

1861

GAGGAAACCA

TCACCCCCTG

GAACTTCGAG

GAAGTGGTGG

ACAAGGGCGC

TTCCGCCCAG

1921

AGCTTCATCG

AGCGGATGAC

CAACTTCGAT

AAGAACCTGC

CCAACGAGAA

GGTGCTGCCC

1981

AAGCACAGCC

TGCTGTACGA

GTACTTCACC

GTGTATAACG

AGCTGACCAA

AGTGAAATAC

2041

GTGACCGAGG

GAATGAGAAA

GCCCGCCTTC

CTGAGCGGCG

AGCAGAAAAA

GGCCATCGTG

2101

GACCTGCTGT

TCAAGACCAA

CCGGAAAGTG

ACCGTGAAGC

AGCTGAAAGA

GGACTACTTC

2161

AAGAAAATCG

AGTGCTTCGA

CTCCGTGGAA

ATCTCCGGCG

TGGAAGATCG

GTTCAACGCC

2221

TCCCTGGGCA

CATACCACGA

TCTGCTGAAA

ATTATCAAGG

ACAAGGACTT

CCTGGACAAT

2281

GAGGAAAACG

AGGACATTCT

GGAAGATATC

GTGCTGACCC

TGACACTGTT

TGAGGACAGA

2341

GAGATGATCG

AGGAACGGCT

GAAAACCTAT

GCCCACCTGT

TCGACGACAA

AGTGATGAAG

2401

CAGCTGAAGC

GGCGGAGATA

CACCGGCTGG

GGCAGGCTGA

GCCGGAAGCT

GATCAACGGC

2461

ATCCGGGACA

AGCAGTCCGG

CAAGACAATC

CTGGATTTCC

TGAAGTCCGA

CGGCTTCGCC

2521

AACAGAAACT

TCATGCAGCT

GATCCACGAC

GACAGCCTGA

CCTTTAAAGA

GGACATCCAG

2581

AAAGCCCAGG

TGTCCGGCCA

GGGCGATAGC

CTGCACGAGC

ACATTGCCAA

TCTGGCCGGC

2641

AGCCCCGCCA

TTAAGAAGGG

CATCCTGCAG

ACAGTGAAGG

TGGTGGACGA

GCTCGTGAAA

2701

GTGATGGGCC

GGCACAAGCC

CGAGAACATC

GTGATCGAAA

TGGCCAGAGA

GAACCAGACC

2761

ACCCAGAAGG

GACAGAAGAA

CAGCCGCGAG

AGAATGAAGC

GGATCGAAGA

GGGCATCAAA

2821

GAGCTGGGCA

GCCAGATCCT

GAAAGAACAC

CCCGTGGAAA

ACACCCAGCT

GCAGAACGAG

2881

AAGCTGTACC

TGTACTACCT

GCAGAATGGG

CGGGATATGT

ACGTGGACCA

GGAACTGGAC

2941

ATCAACCGGC

TGTCCGACTA

CGATGTGGAC

CATATCGTGC

CTCAGAGCTT

TCTGAAGGAC

3001

GACTCCATCG

ACAACAAGGT

GCTGACCAGA

AGCGACAAGA

ACCGGGGCAA

GAGCGACAAC

3061

GTGCCCTCCG

AAGAGGTCGT

GAAGAAGATG

AAGAACTACT

GGCGGCAGCT

GCTGAACGCC

3121

AAGCTGATTA

CCCAGAGAAA

GTTCGACAAT

CTGACCAAGG

CCGAGAGAGG

CGGCCTGAGC

3181

GAACTGGATA

AGGCCGGCTT

CATCAAGAGA

CAGCTGGTGG

AAACCCGGCA

GATCACAAAG

3241

CACGTGGCAC

AGATCCTGGA

CTCCCGGATG

AACACTAAGT

ACGACGAGAA

TGACAAGCTG

3301

ATCCGGGAAG

TGAAAGTGAT

CACCCTGAAG

TCCAAGCTGG

TGTCCGATTT

CCGGAAGGAT

3361

TTCCAGTTTT

ACAAAGTGCG

CGAGATCAAC

AACTACCACC

ACGCCCACGA

CGCCTACCTG

3421

AACGCCGTCG

TGGGAACCGC

CCTGATCAAA

AAGTACCCTA

AGCTGGAAAG

CGAGTTCGTG

3481

TACGGCGACT

ACAAGGTGTA

CGACGTGCGG

AAGATGATCG

CCAAGAGCGA

GCAGGAAATC

3541

GGCAAGGCTA

CCGCCAAGTA

CTTCTTCTAC

AGCAACATCA

TGAACTTTTT

CAAGACCGAG

3601

ATTACCCTGG

CCAACGGCGA

GATCCGGAAG

CGGCCTCTGA

TCGAGACAAA

CGGCGAAACC

3661

GGGGAGATCG

TGTGGGATAA

GGGCCGGGAT

TTTGCCACCG

TGCGGAAAGT

GCTGAGCATG

3721

CCCCAAGTGA

ATATCGTGAA

AAAGACCGAG

GTGCAGACAG

GCGGCTTCAG

CAAAGAGTCT

3781

ATCCTGCCCA

AGAGGAACAG

CGATAAGCTG

ATCGCCAGAA

AGAAGGACTG

GGACCCTAAG

3841

AAGTACGGCG

GCTTCGACAG

CCCCACCGTG

GCCTATTCTG

TGCTGGTGGT

GGCCAAAGTG

3901

GAAAAGGGCA

AGTCCAAGAA

ACTGAAGAGT

GTGAAAGAGC

TGCTGGGGAT

CACCATCATG

3961

GAAAGAAGCA

GCTTCGAGAA

GAATCCCATC

GACTTTCTGG

AAGCCAAGGG

CTACAAAGAA

4021

GTGAAAAAGG

ACCTGATCAT

CAAGCTGCCT

AAGTACTCCC

TGTTCGAGCT

GGAAAACGGC

4081

CGGAAGAGAA

TGCTGGCCTC

TGCCGGCGAA

CTGCAGAAGG

GAAACGAACT

GGCCCTGCCC

4141

TCCAAATATG

TGAACTTCCT

GTACCTGGCC

AGCCACTATG

AGAAGCTGAA

GGGCTCCCCC

4201

GAGGATAATG

AGCAGAAACA

GCTGTTTGTG

GAACAGCACA

AGCACTACCT

GGACGAGATC

4261

ATCGAGCAGA

TCAGCGAGTT

CTCCAAGAGA

GTGATCCTGG

CCGACGCTAA

TCTGGACAAA

4321

GTGCTGTCCG

CCTACAACAA

GCACCGGGAT

AAGCCCATCA

GAGAGCAGGC

CGAGAATATC

4381

ATCCACCTGT

TTACCCTGAC

CAATCTGGGA

GCCCCTGCCG

CCTTCAAGTA

CTTTGACACC

4441

ACCATCGACC

GGAAGAGGTA

CACCAGCACC

AAAGAGGTGC

TGGACGCCAC

CCTGATCCAC

4501

CAGAGCATCA

CCGGCCTGTA

CGAGACACGG

ATCGACCTGT

CTCAGCTGGG

AGGCGACAGC

4561

CCCAAGAAGA

AGAGAAAGGT

GGAGGCCAGC

TAAGAATTCA

ATAAAAGATC

TTTATTTTCA

4621

TTAGATCTGT

GTGTTGGTTT

TTTGTGTGCG

GCCGCAGGAA

CCCCTAGTGA

TGGAGTTGGC

4681

CACTCCCTCT

CTGCGCGCTC

GCTCGCTCAC

TGAGGCCGGG

CGACCAAAGG

TCGCCCGACG

4741

CCCGGGCTTT

GCCCGGGCGG

CCTCAGTGAG

CGAGCGAGCG

CGCAGCTGCC

TGCAGGGGCG

4801

CCTGATGCGG

TATTTTCTCC

TTACGCATCT

GTGCGGTATT

TCACACCGCA

TACGTCAAAG

4861

CAACCATAGT

ACGCGCCCTG

TAGCGGCGCA

TTAAGCGCGG

CGGGTGTGGT

GGTTACGCGC

4921

AGCGTGACCG

CTACACTTGC

CAGCGCCCTA

GCGCCCGCTC

CTTTCGCTTT

CTTCCCTTCC

4981

TTTCTCGCCA

CGTTCGCCGG

CTTTCCCCGT

CAAGCTCTAA

ATCGGGGGCT

CCCTTTAGGG

5041

TTCCGATTTA

GTGCTTTACG

GCACCTCGAC

CCCAAAAAAC

TTGATTTGGG

TGATGGTTCA

5101

CGTAGTGGGC

CATCGCCCTG

ATAGACGGTT

TTTCGCCCTT

TGACGTTGGA

GTCCACGTTC

5161

TTTAATAGTG

GACTCTTGTT

CCAAACTGGA

ACAACACTCA

ACCCTATCTC

GGGCTATTCT

5221

TTTGATTTAT

AAGGGATTTT

GCCGATTTCG

GCCTATTGGT

TAAAAAATGA

GCTGATTTAA

5281

CAAAAATTTA

ACGCGAATTT

TAACAAAATA

TTAACGTTTA

CAATTTTATG

GTGCACTCTC

5341

AGTACAATCT

GCTCTGATGC

CGCATAGTTA

AGCCAGCCCC

GACACCCGCC

AACACCCGCT

5401

GACGCGCCCT

GACGGGCTTG

TCTGCTCCCG

GCATCCGCTT

ACAGACAAGC

TGTGACCGTC

5461

TCCGGGAGCT

GCATGTGTCA

GAGGTTTTCA

CCGTCATCAC

CGAAACGCGC

GAGACGAAAG

5521

GGCCTCGTGA

TACGCCTATT

TTTATAGGTT

AATGTCATGA

TAATAATGGT

TTCTTAGACG

5581

TCAGGTGGCA

CTTTTCGGGG

AAATGTGCGC

GGAACCCCTA

TTTGTTTATT

TTTCTAAATA

5641

CATTCAAATA

TGTATCCGCT

CATGAGACAA

TAACCCTGAT

AAATGCTTCA

ATAATATTGA

5701

AAAAGGAAGA

GTATGAGTAT

TCAACATTTC

CGTGTCGCCC

TTATTCCCTT

TTTTGCGGCA

5761

TTTTGCCTTC

CTGTTTTTGC

TCACCCAGAA

ACGCTGGTGA

AAGTAAAAGA

TGCTGAAGAT

5821

CAGTTGGGTG

CACGAGTGGG

TTACATCGAA

CTGGATCTCA

ACAGCGGTAA

GATCCTTGAG

5881

AGTTTTCGCC

CCGAAGAACG

TTTTCCAATG

ATGAGCACTT

TTAAAGTTCT

GCTATGTGGC

5941

GCGGTATTAT

CCCGTATTGA

CGCCGGGCAA

GAGCAACTCG

GTCGCCGCAT

ACACTATTCT

6001

CAGAATGACT

TGGTTGAGTA

CTCACCAGTC

ACAGAAAAGC

ATCTTACGGA

TGGCATGACA

6061

GTAAGAGAAT

TATGCAGTGC

TGCCATAACC

ATGAGTGATA

ACACTGCGGC

CAACTTACTT

6121

CTGACAACGA

TCGGAGGACC

GAAGGAGCTA

ACCGCTTTTT

TGCACAACAT

GGGGGATCAT

6181

GTAACTCGCC

TTGATCGTTG

GGAACCGGAG

CTGAATGAAG

CCATACCAAA

CGACGAGCGT

6241

GACACCACGA

TGCCTGTAGC

AATGGCAACA

ACGTTGCGCA

AACTATTAAC

TGGCGAACTA

6301

CTTACTCTAG

CTTCCCGGCA

ACAATTAATA

GACTGGATGG

AGGCGGATAA

AGTTGCAGGA

6361

CCACTTCTGC

GCTCGGCCCT

TCCGGCTGGC

TGGTTTATTG

CTGATAAATC

TGGAGCCGGT

6421

GAGCGTGGAA

GCCGCGGTAT

CATTGCAGCA

CTGGGGCCAG

ATGGTAAGCC

CTCCCGTATC

6481

GTAGTTATCT

ACACGACGGG

GAGTCAGGCA

ACTATGGATG

AACGAAATAG

ACAGATCGCT

6541

GAGATAGGTG

CCTCACTGAT

TAAGCATTGG

TAACTGTCAG

ACCAAGTTTA

CTCATATATA

6601

CTTTAGATTG

ATTTAAAACT

TCATTTTTAA

TTTAAAAGGA

TCTAGGTGAA

GATCCTTTTT

6661

GATAATCTCA

TGACCAAAAT

CCCTTAACGT

GAGTTTTCGT

TCCACTGAGC

GTCAGACCCC

6721

GTAGAAAAGA

TCAAAGGATC

TTCTTGAGAT

CCTTTTTTTC

TGCGCGTAAT

CTGCTGCTTG

6781

CAAACAAAAA

AACCACCGCT

ACCAGCGGTG

GTTTGTTTGC

CGGATCAAGA

GCTACCAACT

6841

CTTTTTCCGA

AGGTAACTGG

CTTCAGCAGA

GCGCAGATAC

CAAATACTGT

CCTTCTAGTG

6901

TAGCCGTAGT

TAGGCCACCA

CTTCAAGAAC

TCTGTAGCAC

CGCCTACATA

CCTCGCTCTG

6961

CTAATCCTGT

TACCAGTGGC

TGCTGCCAGT

GGCGATAAGT

CGTGTCTTAC

CGGGTTGGAC

7021

TCAAGACGAT

AGTTACCGGA

TAAGGCGCAG

CGGTCGGGCT

GAACGGGGGG

TTCGTGCACA

7081

CAGCCCAGCT

TGGAGCGAAC

GACCTACACC

GAACTGAGAT

ACCTACAGCG

TGAGCTATGA

7141

GAAAGCGCCA

CGCTTCCCGA

AGGGAGAAAG

GCGGACAGGT

ATCCGGTAAG

CGGCAGGGTC

7201

GGAACAGGAG

AGCGCACGAG

GGAGCTTCCA

GGGGGAAACG

CCTGGTATCT

TTATAGTCCT

7261

GTCGGGTTTC

GCCACCTCTG

ACTTGAGCGT

CGATTTTTGT

GATGCTCGTC

AGGGGGGCGG

7321

AGCCTATGGA

AAAACGCCAG

CAACGCGGCC

TTTTTACGGT

TCCTGGCCTT

TTGCTGGCCT

7381

TTTGCTCACA

TGT

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