名称: | pLV3-CMV-Pla2g7(rat)-3×FLAG-CopGFP-Puro |
---|---|
别称: | NM_001009353.1; |
基因长: | 1320bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 大鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-Pla2g7(rat)-3×FLAG-CopGFP-Puro是适用于哺乳细胞过表达质粒。质粒带有的CMV强启动子可启动下游基因转录,促进基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带的CopGFP绿色荧光蛋白用于观察目的基因的表达和定位情况。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGG
TGCCGCTCAA
ACTGCGCGTG
CTTTTCTGCC
TCCTCTGCTG
CCTCCCACGG
GTACATCCTT
TGTACTGGCA
AGACCCTTCT
TTTTTCGACT
TCAGGCCATC
AGTAATGTTT
CATAAGCTCC
AATCGGTGAT
GTCTGCTGTC
AGCGCTGGCC
GTTGTAAAAT
CCCCAAAGGA
AATGGATCTT
ACCCTGTCGG
CTGTACAGAC
ATGATGTTCG
GTTATGGGAA
TGAGAGCATC
TTCTTGCGTT
TGTACTACCC
AGCTCAAGAT
CAAGGTCCTC
ATGACACTGT
TTGGGTCCCA
AACATAGAAT
ATTTTTGGGG
TCTTAGTAAA
TTTCTCGGAA
CACCTACTTT
TGTAGGCAAC
ATTTTGCGCC
TCTTATATGG
TTCTCTGACA
GCTCCTGCAA
GCTGGAATTT
TCCTTTGCGG
ACTGGAGAAA
AATACCCACT
AATTATCTTT
TCTCACGGTC
TCGGAGCCTT
CAGGACAATT
TATTCTGCTA
TTGGCGCTGC
TTTGGCATCC
TATGGGTTTA
TAGTGGCCAC
TGTGGAACAT
AGAGACGGAT
CTGCATCCGC
AACTTACTAT
TTTGAAGACC
AGGCAGCTGC
GAAAATGGAA
AACAGGTCTT
GGTTTTACCT
GAAAAAGATA
AAACAAGAGG
AGTCGGAAAG
AGCTCGGAAA
GAGCAGGTTC
GGCAGAGAGC
AAAAGAATGT
TCCAAGGCTC
TCAGTGCAAT
TCTTGACATT
GAACATGGAA
ACCCAAAAGA
GAATGTACTA
GGTTTACCTT
TTGATATGAA
ACAGCTGAAG
GATTCTATCG
ATGAGACTAA
AATAGCTGTG
ATGGGGCATT
CTTTTGGAGG
AGCAACAGTT
TTTCAAGCCC
TAAGTGAAGA
CCAGAGATTC
AGATGTGGGA
TTGCCCTTGA
TCCGTGGATG
TTTCCCGTGA
GTGAGGAGCT
GTACTCCAGA
GTTCCTCAGC
CTCTCTTCTT
TATCAACTCT
GCCGAATTCC
AGACTCCAAA
GGACATTGCA
AAAATGAAAA
ACTTCTACCA
GCCTGACAAG
GAAAGGAAAA
TGATTACGAT
CAAGGGCTCA
GTGCACCAGA
ATTTTGCTGA
CGGGACTTTT
GTAACTGGCA
AAATAATTGG
AAACAAGCTG
TCACTGAAAG
GAGACATAGA
CTCCAGAGTT
GCCATAGACC
TCACCAACAA
GGCTTCCTTG
GCTTTCTTAC
AAAAACATTT
AGGACTTCAT
AAAGACTTTG
ATCAGTGGGA
CTGTCTGGTG
GAGGGAGAGA
ACGAGAACCT
CATCCCGGGG
TCACCCTTTG
ATGTAGTCAC
CCAGTCCCCG
GCTCTGCAGA
GTTCTCCCGG
ATCACACAAC
CAGAATGGAT
CCGACTACAA
AGACCATGAC
GGTGATTATA
AAGATCATGA
CATCGACTAC
AAGGATGACG
ATGACAAGTA
GCGGCCGCGA
AGGATCTGCG
ATCGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACGGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGCTGAAGC
TTCGAGGGGC
TCGCATCTCT
CCTTCACGCG
CCCGCCGCCC
TACCTGAGGC
CGCCATCCAC
GCCGGTTGAG
TCGCGTTCTG
CCGCCTCCCG
CCTGTGGTGC
CTCCTGAACT
GCGTCCGCCG
TCTAGGTAAG
TTTAAAGCTC
AGGTCGAGAC
CGGGCCTTTG
TCCGGCGCTC
CCTTGGAGCC
TACCTAGACT
CAGCCGGCTC
TCCACGCTTT
GCCTGACCCT
GCTTGCTCAA
CTCTACGTCT
TTGTTTCGTT
TTCTGTTCTG
CGCCGTTACA
GATCCAAGCT
GTGACCGGCG
CCTACGCTAG
ACGCCACCAT
GGAGAGCGAC
GAGAGCGGCC
TGCCCGCCAT
GGAGATCGAG
TGCCGCATCA
CCGGCACCCT
GAACGGCGTG
GAGTTCGAGC
TGGTGGGCGG
CGGAGAGGGC
ACCCCCAAGC
AGGGCCGCAT
GACCAACAAG
ATGAAGAGCA
CCAAAGGCGC
CCTGACCTTC
AGCCCCTACC
TGCTGAGCCA
CGTGATGGGC
TACGGCTTCT
ACCACTTCGG
CACCTACCCC
AGCGGCTACG
AGAACCCCTT
CCTGCACGCC
ATCAACAACG
GCGGCTACAC
CAACACCCGC
ATCGAGAAGT
ACGAGGACGG
CGGCGTGCTG
CACGTGAGCT
TCAGCTACCG
CTACGAGGCC
GGCCGCGTGA
TCGGCGACTT
CAAGGTGGTG
GGCACCGGCT
TCCCCGAGGA
CAGCGTGATC
TTCACCGACA
AGATCATCCG
CAGCAACGCC
ACCGTGGAGC
ACCTGCACCC
CATGGGCGAT
AACGTGCTGG
TGGGCAGCTT
CGCCCGCACC
TTCAGCCTGC
GCGACGGCGG
CTACTACAGC
TTCGTGGTGG
ACAGCCACAT
GCACTTCAAG
AGCGCCATCC
ACCCCAGCAT
CCTGCAGAAC
GGGGGCCCCA
TGTTCGCCTT
CCGCCGCGTG
GAGGAGCTGC
ACAGCAACAC
CGAGCTGGGC
ATCGTGGAGT
ACCAGCACGC
CTTCAAGACC
CCCATCGCCT
TCGCCAGATC
CCGCGCTCAG
TCGTCCAATT
CTGCCGTGGA
CGGCACCGCC
GGACCCGGCT
CCACCGGATC
TCGCGAGGGC
AGAGGAAGTC
TTCTAACATG
CGGTGACGTG
GAGGAGAATC
CCGGCCCTAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCA
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ATCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAATCATCG
TCCTTTCCTT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTTCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGGCCGCC
TCCCCGCCTG
GTACCTTTAA
GACCAATGAC
TTACAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAGAT
AAGATCTGCT
TTTTGCTTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTAGTA
GTTCATGTCA
TCTTATTATT
CAGTATTTAT
AACTTGCAAA
GAAATGAATA
TCAGAGAGTG
AGAGGAACTT
GTTTATTGCA
GCTTATAATG
GTTACAAATA
AAGCAATAGC
ATCACAAATT
TCACAAATAA
AGCATTTTTT
TCACTGCATT
CTAGTTGTGG
TTTGTCCAAA
CTCATCAATG
TATCTTATCA
TGTCTGGCTC
TAGCTATCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGACTTT
TGCAGAGACC
AAATTCGTAA
TCATGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGGAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCTAAGAAAC
CATTATTATC
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTCTCGC
GCGTTTCGGT
GATGACGGTG
AAAACCTCTG
ACACATGCAG
CTCCCGGAGA
CGGTCACAGC
TTGTCTGTAA
GCGGATGCCG
GGAGCAGACA
AGCCCGTCAG
GGCGCGTCAG
CGGGTGTTGG
CGGGTGTCGG
GGCTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGCGCCATTC
GCCATTCAGG
CTGCGCAACT
GTTGGGAAGG
GCGATCGGTG
CGGGCCTCTT
CGCTATTACG
CCAGCTGGCG
AAAGGGGGAT
GTGCTGCAAG
GCGATTAAGT
TGGGTAACGC
CAGGGTTTTC
CCAGTCACGA
CGTTGTAAAA
CGACGGCCAG
TGCCAAGCTG