产品货号：P72617
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pRetroX-Tbx21(mouse)-mCherry-Puro |
|---|---|
| 别称: | NM_019507.2;Tbet; Tblym; TBT1 |
| 基因长: | 1593bp |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 小鼠 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 红色 |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pECMV-Tbx21-m-FLAG |
| 正向引物: | pRetroX-F1：TGGTTCATTCTCAAGCCTCAGA |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pRetroX-Tbx21(mouse)-mCherry-Puro是适用于哺乳细胞过表达质粒。质粒带有的CMV增强子和CMV启动子可联合启动下游基因高效表达，质粒在基因C端携带红色荧光蛋白，便于直观地观察目的基因表达的位置和水平等。质粒携带Puro抗性基因，可用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
TTCATCAATA
ATATACCTTA
TTTTGGATTG
AAGCCAATAT
GATAATGAGG
GGGTGGAGTT
TGTGACGTGG
CGCGGGGCGT
GGGAACGGGG
CGGGTGACGT
AGTAGTGTGG
CGGAAGTGTG
ATGTTGCAAG
TGTGGCGGAA
CACATGTAAG
CGACGGATGT
GGCAAAAGTG
ACGTTTTTGG
TGTGCGCCGG
TGTACACAGG
AAGTGACAAT
TTTCGCGCGG
TTTTAGGCGG
ATGTTGTAGT
AAATTTGGGC
GTAACCGAGT
AAGATTTGGC
CATTTTCGCG
GGAAAACTGA
ATAAGAGGAA
GTGAAATCTG
AATAATTTTG
TGTTACTCAT
AGCGCGTAAT
ATTTGTCTAG
GGCCGCGGGG
ACTTTGACCG
TTTACGTGGA
GACTCGCCCA
GGTGTTTTTC
TCAGGTGTTT
TCCGCGTTCC
GGGTCAAAGT
TGGCGTTTTA
TTATTATAGT
CAGTCTAGAG
ATATACTGAG
TCATTAGGGA
CTTTCCAATG
GGTTTTGCCC
AGTACATAAG
GTCAATAGGG
GTGAATCAAC
AGGAAAGTCC
CATTGGAGCC
AAGTACACTG
AGTCAATAGG
GACTTTCCAT
TGGGTTTTGC
CCAGTACAAA
AGGTCAATAG
GGGGTGAGTC
AATGGGTTTT
TCCCATTATT
GGCACGTACA
TAAGGTCAAT
AGGGGTGAGT
CATTGGGTTT
TTCCAGCCAA
TTTAATTAAA
ACGCCATGTA
CTTTCCCACC
ATTGACGTCA
ATGGGCTATT
GAAACTAATG
CAACGTGACC
TTTAAACGGT
ACTTTCCCAT
AGCTGATTAA
TGGGAAAGTA
CCGTTCTCGA
GCCAATACAC
GTCAATGGGA
AGTGAAAGGG
CAGCCAAAAC
GTAACACCGC
CCCGGTTTTC
CCCTGGAAAT
TCCATATTGG
CACGCATTCT
ATTGGCTGAG
CTGCGTTCTA
CGTGGGTATA
AGAGGCGCGA
CCAGCGTCGG
TACCGTCGCA
GTCTTCGGTC
TGACCACCGT
AGAACGCAGA
TCGAATTCAT
GGTGAGCAAG
GGCGAGGAGC
TGTTCACCGG
GGTGGTGCCC
ATCCTGGTCG
AGCTGGACGG
CGACGTAAAC
GGCCACAAGT
TCAGCGTGTC
CGGCGAGGGC
GAGGGCGATG
CCACCTACGG
CAAGCTGACC
CTGAAGTTCA
TCTGCACCAC
CGGCAAGCTG
CCCGTGCCCT
GGCCCACCCT
CGTGACCACC
CTGACCTACG
GCGTGCAGTG
CTTCAGCCGC
TACCCCGACC
ACATGAAGCA
GCACGACTTC
TTCAAGTCCG
CCATGCCCGA
AGGCTACGTC
CAGGAGCGCA
CCATCTTCTT
CAAGGACGAC
GGCAACTACA
AGACCCGCGC
CGAGGTGAAG
TTCGAGGGCG
ACACCCTGGT
GAACCGCATC
GAGCTGAAGG
GCATCGACTT
CAAGGAGGAC
GGCAACATCC
TGGGGCACAA
GCTGGAGTAC
AACTACAACA
GCCACAACGT
CTATATCATG
GCCGACAAGC
AGAAGAACGG
CATCAAGGTG
AACTTCAAGA
TCCGCCACAA
CATCGAGGAC
GGCAGCGTGC
AGCTCGCCGA
CCACTACCAG
CAGAACACCC
CCATCGGCGA
CGGCCCCGTG
CTGCTGCCCG
ACAACCACTA
CCTGAGCACC
CAGTCCGCCC
TGAGCAAAGA
CCCCAACGAG
AAGCGCGATC
ACATGGTCCT
GCTGGAGTTC
GTGACCGCCG
CCGGGATCAC
TCTCGGCATG
GACGAGCTGT
ACAAGTCCGG
ACTCAGATCT
CGAGATCCAC
CGGATCTAGA
TAACTGATCA
TAATCAGCCA
TACCACATTT
GTAGAGGTTT
TACTTGCTTT
AAAAAACCTC
CCACACCTCC
CCCTGAACCT
GAAACATAAA
ATGAATGCAA
TTGTTGTTGT
TAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTAACGCGTT
GACATTGATT
ATTGACTAGT
TATTAATAGT
AATCAATTAC
GGGGTCATTA
GTTCATAGCC
CATATATGGA
GTTCCGCGTT
ACATAACTTA
CGGTAAATGG
CCCGCCTGGC
TGACCGCCCA
ACGACCCCCG
CCCATTGACG
TCAATAATGA
CGTATGTTCC
CATAGTAACG
CCAATAGGGA
CTTTCCATTG
ACGTCAATGG
GTGGAGTATT
TACGGTAAAC
TGCCCACTTG
GCAGTACATC
AAGTGTATCA
TATGCCAAGT
ACGCCCCCTA
TTGACGTCAA
TGACGGTAAA
TGGCCCGCCT
GGCATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTCT
ATATAAGCAG
AGCTCTCTGG
CTAACTAGAG
AACCCACTGC
TTACTGGCTT
ATCGAAATTA
ATACGACTCA
CTATAGGGAG
ACCCAAGCTG
GCTAGCGTTT
AAACGGGCCC
TCTAGACTCG
AGCGGCCGCC
ACTGTGCTGG
ATATCGCCAC
CATGAGCACA
GGTGATAGTT
TCGAGACAAG
ATTCGAGAAA
ATGGATAATC
TGCTGAGGGA
CCCCAAAAGT
GAAGTTAACT
CCGATTGCCT
GCTTGATGGT
CTGGACGCCC
TTGTCTATGA
CTTGGACTTC
CCCGCCCTTC
GCAAAAACAA
GAACATTGAC
AACTTCCTGT
CTAGGTATAA
GGACACCATC
AACAAGATAA
GGGATCTCCG
CATGAAGGCC
GAAGATTACG
AGGTGGTTAA
GGTGATCGGA
CGCGGTGCTT
TTGGAGAGGT
GCAGCTTGTG
CGGCACAAAT
CAACAAGGAA
GGTTTACGCG
ATGAAGCTTC
TCAGCAAATT
TGAAATGATA
AAGAGATCTG
ATTCTGCTTT
TTTCTGGGAA
GAAAGGGACA
TCATGGCTTT
TGCCAACAGT
CCTTGGGTTG
TTCAGCTTTT
TTATGCATTC
CAAGATGATC
GTTATCTCTA
CATGGTGATG
GAATACATGC
CTGGTGGAGA
TCTTGTAAAC
TTAATGAGCA
ACTATGATGT
GCCTGAAAAA
TGGGCACGAT
TCTATACTGC
AGAAGTAGTT
CTTGCATTGG
ATGCAATCCA
TTCCATGGGT
TTTATTCACA
GAGATGTGAA
GCCTGATAAC
ATGCTGCTGG
ATAAATCTGG
ACATTTGAAG
TTAGCAGATT
TTGGTACTTG
TATGAAGATG
AATAAGGAAG
GCATGGTACG
ATGTGATACA
GCGGTTGGAA
CACCTGATTA
TATTTCCCCT
GAAGTATTAA
AATCCCAAGG
TGGTGATGGT
TATTATGGAA
GAGAATGTGA
CTGGTGGTCG
GTTGGGGTAT
TTTTATACGA
AATGCTTGTA
GGTGATACAC
CTTTTTATGC
AGATTCTTTG
GTTGGAACTT
ACAGTAAAAT
TATGAACCAT
AAAAATTCAC
TTACCTTTCC
TGATGATAAT
GACATATCAA
AAGAAGCAAA
AAACCTTATT
TGTGCCTTCC
TTACTGACAG
GGAAGTGAGG
TTAGGGCGAA
ATGGTGTAGA
AGAAATCAAA
CGACATCTCT
TCTTCAAAAA
TGACCAGTGG
GCTTGGGAAA
CGCTCCGAGA
CACTGTAGCA
CCAGTTGTAC
CCGATTTAAG
TAGTGACATT
GATACTAGTA
ATTTTGATGA
CTTGGAAGAA
GATAAAGGAG
AGGAAGAAAC
ATTCCCTATT
CCTAAAGCTT
TCGTTGGCAA
TCAACTACCT
TTTGTAGGAT
TTACATATTA
TAGCAATCGT
AGATACTTAT
CTTCAGCAAA
TCCTAATGAT
AACAGAACTA
GCTCCAATGC
AGATAAAAGC
TTGCAGGAAA
GTTTGCAAAA
AACAATCTAT
AAGCTGGAAG
AACAGCTGCA
TAATGAAATG
CAGTTAAAAG
ATGAAATGGA
GCAGAAGTGC
AGAACCTCAA
ACATAAAACT
AGACAAGATA
ATGAAAGAAT
TGGATGAAGA
GGGAAATCAA
AGAAGAAATC
TAGAATCTAC
AGTGTCTCAG
ATTGAGAAGG
AGAAAATGTT
GCTACAGCAT
AGAATTAATG
AGTACCAAAG
AAAAGCTGAA
CAGGAAAATG
AGAAGAGAAG
AAATGTAGAA
AATGAAGTTT
CTACATTAAA
GGATCAGTTG
GAAGACTTAA
AGAAAGTCAG
TCAGAATTCA
CAGCTTGCTA
ATGAGAAGCT
GTCCCAGTTA
CAAAAGCAGC
TAGAAGAAGC
CAATGACTTA
CTTAGGACAG
AATCGGACAC
AGCTGTAAGA
TTGAGGAAGA
GTCACACAGA
GATGAGCAAG
TCAATTAGTC
AGTTAGAGTC
CCTGAACAGA
GAGTTGCAAG
AGAGAAATCG
AATTTTAGAG
AATTCTAAGT
CACAAACAGA
CAAAGATTAT
TACCAGCTGC
AAGCTATATT
AGAAGCTGAA
CGAAGAGACA
GAGGTCATGA
TTCTGAGATG
ATTGGAGACC
TTCAAGCTCG
AATTACATCT
TTACAAGAGG
AGGTGAAGCA
TCTCAAACAT
AATCTCGAAA
AAGTGGAAGG
AGAAAGAAAA
GAGGCTCAAG
ACATGCTTAA
TCACTCAGAA
AAGGAAAAGA
ATAATTTAGA
GATAGATTTA
AACTACAAAC
TTAAATCATT
ACAACAACGG
TTAGAACAAG
AGGTAAATGA
ACACAAAGTA
ACCAAAGCTC
GTTTAACTGA
CAAACATCAA
TCTATTGAAG
AGGCAAAGTC
TGTGGCAATG
TGTGAGATGG
AAAAAAAGCT
GAAAGAAGAA
AGAGAAGCTC
GAGAGAAGGC
TGAAAATCGG
GTTGTTCAGA
TTGAGAAACA
GTGTTCCATG
CTAGACGTTG
ATCTGAAGCA
ATCTCAGCAG
AAACTAGAAC
ATTTGACTGG
AAATAAAGAA
AGGATGGAGG
ATGAAGTTAA
GAATCTAACC
CTGCAACTGG
AGCAGGAATC
AAATAAGCGG
CTGTTGTTAC
AAAATGAATT
GAAGACTCAA
GCATTTGAGG
CAGACAATTT
AAAAGGTTTA
GAAAAGCAGA
TGAAACAGGA
AATAAATACT
TTATTGGAAG
CAAAGAGATT
ATTAGAATTT
GAGTTAGCTC
AGCTTACGAA
ACAGTATAGA
GGAAATGAAG
GACAGATGCG
GGAGCTACAA
GATCAGCTTG
AAGCTGAGCA
ATATTTCTCG
ACACTTTATA
AAACCCAGGT
AAAGGAACTT
AAAGAAGAAA
TTGAAGAAAA
AAACAGAGAA
AATTTAAAGA
AAATACAGGA
ACTACAAAAT
GAAAAAGAAA
CTCTTGCTAC
TCAGTTGGAT
CTAGCAGAAA
CAAAAGCTGA
GTCTGAGCAG
TTGGCGCGAG
GCCTTCTGGA
AGAACAGTAT
TTTGAATTGA
CGCAAGAAAG
CAAGAAAGCT
GCTTCAAGAA
ATAGACAAGA
GATTACAGAT
AAAGATCACA
CTGTTAGTCG
GCTTGAAGAA
GCAAACAGCA
TGCTAACCAA
AGATATTGAA
ATATTAAGAA
GAGAGAATGA
AGAGCTAACA
GAGAAAATGA
AGAAGGCAGA
GGAAGAATAT
AAACTGGAGA
AGGAGGAGGA
GATCAGTAAT
CTTAAGGCTG
CCTTTGAAAA
GAATATCAAC
ACTGAACGAA
CCCTTAAAAC
ACAGGCTGTT
AACAAATTGG
CAGAAATAAT
GAATCGAAAA
GATTTTAAAA
TTGATAGAAA
GAAAGCTAAT
ACACAAGATT
TGAGAAAGAA
AGAAAAGGAA
AATCGAAAGC
TGCAACTGGA
ACTCAACCAA
GAAAGAGAGA
AATTCAACCA
GATGGTAGTG
AAACATCAGA
AGGAACTGAA
TGACATGCAA
GCGCAATTGG
TAGAAGAATG
TGCACATAGG
AATGAGCTTC
AGATGCAGTT
GGCCAGCAAA
GAGAGTGATA
TTGAGCAATT
GCGTGCTAAA
CTTTTGGACC
TCTCGGATTC
TACAAGTGTT
GCTAGTTTTC
CTAGTGCTGA
TGAAACTGAT
GGTAACCTCC
CAGAGTCAAG
AATTGAAGGT
TGGCTTTCAG
TACCAAATAG
AGGAAATATC
AAACGATATG
GCTGGAAGAA
ACAGTATGTT
GTGGTAAGCA
GCAAAAAAAT
TTTGTTCTAT
AATGACGAAC
AAGATAAGGA
GCAATCCAAT
CCATCTATGG
TATTGGACAT
AGATAAACTG
TTTCACGTTA
GACCTGTAAC
CCAAGGAGAT
GTGTATAGAG
CTGAAACTGA
AGAAATTCCT
AAAATATTCC
AGATACTATA
TGCAAATGAA
GGTGAATGTA
GAAAAGATGT
AGAGATGGAA
CCAGTACAAC
AAGCTGAAAA
AACTAATTTC
CAAAATCACA
AAGGCCATGA
GTTTATTCCT
ACACTCTACC
ACTTTCCTGC
CAATTGTGAT
GCCTGTGCCA
AACCTCTCTG
GCATGTTTTT
AAGCCACCCC
CTGCCCTAGA
GTGTCGAAGA
TGCCATGTTA
AGTGCCACAG
AGATCACTTA
GATAAGAAAG
AGGACTTAAT
TTGTCCATGT
AAAGTAAGTT
ATGATGTAAC
ATCAGCAAGA
GATATGCTGC
TGTTAGCATG
TTCTCAGGAT
GAACAAAAAA
AATGGGTAAC
TCATTTAGTA
AAGAAAATCC
CTAAGAATCC
ACCATCTGGT
TTTGTTCGTG
CTTCCCCTCG
AACGCTTTCT
ACAAGATCCA
CTGCAAATCA
GTCTTTCCGG
AAAGTGGTCA
AAAATACATC
TGGAAAAACT
AGTTAAAAGC
TTAAGTTTAA
ACCGCTGATC
AGCCTCGACT
GTGCCTTCTA
GTTGCCAGCC
ATCTGTTGTT
TGCCCCTCCC
CCGTGCCTTC
CTTGACCCTG
GAAGGTGCCA
CTCCCACTGT
CCTTTCCTAA
TAAAATGAGG
AAATTGCATC
GCATTGTCTG
AGTAGGTGTC
ATTCTATTCT
GGGGGGTGGG
GTGGGGCAGG
ACAGCAAGGG
GGAGGATTGG
GAAGACAATA
GCAGGCATGC
TGGGGATGCG
GTGGGCTCTA
TGGGTCGACT
TCGAGCAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGGAT
CGTCTAGCAT
CGAAGATCCA
ATAACTTCGT
ATAGCATACA
TTATACGAAG
TTATAAGTAG
CTTGGCGTAA
TCATGGTCAT
AGCTGTTTCC
TGTGTGAAAT
TGTTATCCGC
TCACAATTCC
ACACAACATA
CGAGCCGGAA
GCATAAAGTG
TAAAGCCTGG
GGTGCCTAAT
GAGTGAGCTA
ACTCACATTA
ATTGCGTTGC
GCTCACTGCC
CGCTTTCCAG
TCGGGAAACC
TGTCGTGCCA
GCTGCATTAA
TGAATCGGCC
AACGCGCGGG
GAGAGGCGGT
TTGCGTATTG
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CAATGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGGA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
CAATGCTTAA
TCAGTGAGGC
ACCTATCTCA
GCGATCTGTC
TATTTCGTTC
ATCCATAGTT
GCCTGACTCC
CCGTCGTGTA
GATAACTACG
ATACGGGAGG
GCTTACCATC
TGGCCCCAGT
GCTGCAATGA
TACCGCGAGA
CCCACGCTCA
CCGGCTCCAG
ATTTATCAGC
AATAAACCAG
CCAGCCGGAA
GGGCCGAGCG
CAGAAGTGGT
CCTGCAACTT
TATCCGCCTC
CATCCAGTCT
ATTAATTGTT
GCCGGGAAGC
TAGAGTAAGT
AGTTCGCCAG
TTAATAGTTT
GCGCAACGTT
GTTGCCATTG
CTACAGGCAT
CGTGGTGTCA
CGCTCGTCGT
TTGGTATGGC
TTCATTCAGC
TCCGGTTCCC
AACGATCAAG
GCGAGTTACA
TGATCCCCCA
TGTTGTGCAA
AAAAGCGGTT
AGCTCCTTCG
GTCCTCCGAT
CGTTGTCAGA
AGTAAGTTGG
CCGCAGTGTT
ATCACTCATG
GTTATGGCAG
CACTGCATAA
TTCTCTTACT
GTCATGCCAT
CCGTAAGATG
CTTTTCTGTG
ACTGGTGAGT
ACTCAACCAA
GTCATTCTGA
GAATAGTGTA
TGCGGCGACC
GAGTTGCTCT
TGCCCGGCGT
CAACACGGGA
TAATACCGCG
CCACATAGCA
GAACTTTAAA
AGTGCTCATC
ATTGGAAAAC
GTTCTTCGGG
GCGAAAACTC
TCAAGGATCT
TACCGCTGTT
GAGATCCAGT
TCGATGTAAC
CCACTCGTGC
ACCCAACTGA
TCTTCAGCAT
CTTTTACTTT
CACCAGCGTT
TCTGGGTGAG
CAAAAACAGG
AAGGCAAAAT
GCCGCAAAAA
AGGGAATAAG
GGCGACACGG
AAATGTTGAA
TACTCATACT
CTTCCTTTTT
CAATATTATT
GAAGCATTTA
TCAGGGTTAT
TGTCTCATGA
GCGGATACAT
ATTTGAATGT
ATTTAGAAAA
ATAAACAAAT
AGGGGTTCCG
CGCACATTTC
CCCGAAAAGT
GCCACCTGAC
GTCTAAGAAA
CCATTATTAT
CATGACATTA
ACCTATAAAA
ATAGGCGTAT
CACTCTAGGC
AAAATAGCAC
CCTCCCGCTC
CAGAACAACA
TACAGCGCTT
CACAGCGGCA
GCCTAACAGT
CAGCCTTACC
AGTAAAAAAG
AAAACCTATT
AAAAAAACAC
CACTCGACAC
GGCACCAGCT
CAATCAGTCA
CAGTGTAAAA
AAGGGCCAAG
TGCAGAGCGA
GTATATATAG
GACTAAAAAA
TGACGTAACG
GTTAAAGTCC
ACAAAAAACA
CCCAGAAAAC
CGCACGCGAA
CCTACGCCCA
GAAACGAAAG
CCAAAAAACC
CACAACTTCC
TCAAATCGTC
ACTTCCGTTT
TCCCACGTTA
CGTAACTTCC
CATTTTAAGA
AAACTACAAT
TCCCAACACA
TACAAGTTAC
TCCGCCCTAA
AACCTACGTC
ACCCGCCCCG
TTCCCACGCC
CCGCGCCACG
TCACAAACTC
CACCCCCTCA
TTATCATATT
GGCTTCAATC
CAAAATAAGG
TATA
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