名称: | pLV3-U6-Rab26(rat)-sgRNA1-EF1a-Cas9-Neo |
---|---|
别称: | tgactctactcaagaacgag |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 大鼠 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLentiCRISPRV2-Neo |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-U6-Rab26(rat)-sgRNA1-EF1a-Cas9-Neo是适用于哺乳细胞基因编辑的质粒。质粒带有的U6启动子可启动针对Rab26基因设计的sgRNA片段转录,促使Cas9能在sgRNA的引导下精准地识别并切割目标基因的双链 DNA。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于编辑片段稳定整合至宿主细胞。质粒带有Neo/G418抗性基因,可筛选阳性宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGCGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GCGCGTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TGGCGCCCGA
ACAGGGACTT
GAAAGCGAAA
GGGAAACCAG
AGGAGCTCTC
TCGACGCAGG
ACTCGGCTTG
CTGAAGCGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGTACGCCA
AAAATTTTGA
CTAGCGGAGG
CTAGAAGGAG
AGAGATGGGT
GCGAGAGCGT
CAGTATTAAG
CGGGGGAGAA
TTAGATCGCG
ATGGGAAAAA
ATTCGGTTAA
GGCCAGGGGG
AAAGAAAAAA
TATAAATTAA
AACATATAGT
ATGGGCAAGC
AGGGAGCTAG
AACGATTCGC
AGTTAATCCT
GGCCTGTTAG
AAACATCAGA
AGGCTGTAGA
CAAATACTGG
GACAGCTACA
ACCATCCCTT
CAGACAGGAT
CAGAAGAACT
TAGATCATTA
TATAATACAG
TAGCAACCCT
CTATTGTGTG
CATCAAAGGA
TAGAGATAAA
AGACACCAAG
GAAGCTTTAG
ACAAGATAGA
GGAAGAGCAA
AACAAAAGTA
AGACCACCGC
ACAGCAAGCG
GCCGCTGATC
TTCAGACCTG
GAGGAGGAGA
TATGAGGGAC
AATTGGAGAA
GTGAATTATA
TAAATATAAA
GTAGTAAAAA
TTGAACCATT
AGGAGTAGCA
CCCACCAAGG
CAAAGAGAAG
AGTGGTGCAG
AGAGAAAAAA
GAGCAGTGGG
AATAGGAGCT
TTGTTCCTTG
GGTTCTTGGG
AGCAGCAGGA
AGCACTATGG
GCGCAGCGTC
AATGACGCTG
ACGGTACAGG
CCAGACAATT
ATTGTCTGGT
ATAGTGCAGC
AGCAGAACAA
TTTGCTGAGG
GCTATTGAGG
CGCAACAGCA
TCTGTTGCAA
CTCACAGTCT
GGGGCATCAA
GCAGCTCCAG
GCAAGAATCC
TGGCTGTGGA
AAGATACCTA
AAGGATCAAC
AGCTCCTGGG
GATTTGGGGT
TGCTCTGGAA
AACTCATTTG
CACCACTGCT
GTGCCTTGGA
ATGCTAGTTG
GAGTAATAAA
TCTCTGGAAC
AGATTTGGAA
TCACACGACC
TGGATGGAGT
GGGACAGAGA
AATTAACAAT
TACACAAGCT
TAATACACTC
CTTAATTGAA
GAATCGCAAA
ACCAGCAAGA
AAAGAATGAA
CAAGAATTAT
TGGAATTAGA
TAAATGGGCA
AGTTTGTGGA
ATTGGTTTAA
CATAACAAAT
TGGCTGTGGT
ATATAAAATT
ATTCATAATG
ATAGTAGGAG
GCTTGGTAGG
TTTAAGAATA
GTTTTTGCTG
TACTTTCTAT
AGTGAATAGA
GTTAGGCAGG
GATATTCACC
ATTATCGTTT
CAGACCCACC
TCCCAACCCC
GAGGGGACCC
GACAGGCCCG
AAGGAATAGA
AGAAGAAGGT
GGAGAGAGAG
ACAGAGACAG
ATCCATTCGA
TTAGTGAACG
GATCGGCACT
GCGTGCGCCA
ATTCTGCAGA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
AGTTTGGTTA
ATTAAGGTAC
CGAGGGCCTA
TTTCCCATGA
TTCCTTCATA
TTTGCATATA
CGATACAAGG
CTGTTAGAGA
GATAATTAGA
ATTAATTTGA
CTGTAAACAC
AAAGATATTA
GTACAAAATA
CGTGACGTAG
AAAGTAATAA
TTTCTTGGGT
AGTTTGCAGT
TTTAAAATTA
TGTTTTAAAA
TGGACTATCA
TATGCTTACC
GTAACTTGAA
AGTATTTCGA
TTTCTTGGCT
TTATATATCT
TGTGGAAAGG
ACGAAACACC
GTGACTCTAC
TCAAGAACGA
GGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TTTGAATTCG
CTAGCTAGGT
CTTGAAAGGA
GTGGGAATTG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGATCC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GACCGGTTCT
AGAGCGCTGC
CACCATGGAC
AAGAAGTACA
GCATCGGCCT
GGACATCGGC
ACCAACTCTG
TGGGCTGGGC
CGTGATCACC
GACGAGTACA
AGGTGCCCAG
CAAGAAATTC
AAGGTGCTGG
GCAACACCGA
CCGGCACAGC
ATCAAGAAGA
ACCTGATCGG
AGCCCTGCTG
TTCGACAGCG
GCGAAACAGC
CGAGGCCACC
CGGCTGAAGA
GAACCGCCAG
AAGAAGATAC
ACCAGACGGA
AGAACCGGAT
CTGCTATCTG
CAAGAGATCT
TCAGCAACGA
GATGGCCAAG
GTGGACGACA
GCTTCTTCCA
CAGACTGGAA
GAGTCCTTCC
TGGTGGAAGA
GGATAAGAAG
CACGAGCGGC
ACCCCATCTT
CGGCAACATC
GTGGACGAGG
TGGCCTACCA
CGAGAAGTAC
CCCACCATCT
ACCACCTGAG
AAAGAAACTG
GTGGACAGCA
CCGACAAGGC
CGACCTGCGG
CTGATCTATC
TGGCCCTGGC
CCACATGATC
AAGTTCCGGG
GCCACTTCCT
GATCGAGGGC
GACCTGAACC
CCGACAACAG
CGACGTGGAC
AAGCTGTTCA
TCCAGCTGGT
GCAGACCTAC
AACCAGCTGT
TCGAGGAAAA
CCCCATCAAC
GCCAGCGGCG
TGGACGCCAA
GGCCATCCTG
TCTGCCAGAC
TGAGCAAGAG
CAGACGGCTG
GAAAATCTGA
TCGCCCAGCT
GCCCGGCGAG
AAGAAGAATG
GCCTGTTCGG
AAACCTGATT
GCCCTGAGCC
TGGGCCTGAC
CCCCAACTTC
AAGAGCAACT
TCGACCTGGC
CGAGGATGCC
AAACTGCAGC
TGAGCAAGGA
CACCTACGAC
GACGACCTGG
ACAACCTGCT
GGCCCAGATC
GGCGACCAGT
ACGCCGACCT
GTTTCTGGCC
GCCAAGAACC
TGTCCGACGC
CATCCTGCTG
AGCGACATCC
TGAGAGTGAA
CACCGAGATC
ACCAAGGCCC
CCCTGAGCGC
CTCTATGATC
AAGAGATACG
ACGAGCACCA
CCAGGACCTG
ACCCTGCTGA
AAGCTCTCGT
GCGGCAGCAG
CTGCCTGAGA
AGTACAAAGA
GATTTTCTTC
GACCAGAGCA
AGAACGGCTA
CGCCGGCTAC
ATTGACGGCG
GAGCCAGCCA
GGAAGAGTTC
TACAAGTTCA
TCAAGCCCAT
CCTGGAAAAG
ATGGACGGCA
CCGAGGAACT
GCTCGTGAAG
CTGAACAGAG
AGGACCTGCT
GCGGAAGCAG
CGGACCTTCG
ACAACGGCAG
CATCCCCCAC
CAGATCCACC
TGGGAGAGCT
GCACGCCATT
CTGCGGCGGC
AGGAAGATTT
TTACCCATTC
CTGAAGGACA
ACCGGGAAAA
GATCGAGAAG
ATCCTGACCT
TCCGCATCCC
CTACTACGTG
GGCCCTCTGG
CCAGGGGAAA
CAGCAGATTC
GCCTGGATGA
CCAGAAAGAG
CGAGGAAACC
ATCACCCCCT
GGAACTTCGA
GGAAGTGGTG
GACAAGGGCG
CTTCCGCCCA
GAGCTTCATC
GAGCGGATGA
CCAACTTCGA
TAAGAACCTG
CCCAACGAGA
AGGTGCTGCC
CAAGCACAGC
CTGCTGTACG
AGTACTTCAC
CGTGTATAAC
GAGCTGACCA
AAGTGAAATA
CGTGACCGAG
GGAATGAGAA
AGCCCGCCTT
CCTGAGCGGC
GAGCAGAAAA
AGGCCATCGT
GGACCTGCTG
TTCAAGACCA
ACCGGAAAGT
GACCGTGAAG
CAGCTGAAAG
AGGACTACTT
CAAGAAAATC
GAGTGCTTCG
ACTCCGTGGA
AATCTCCGGC
GTGGAAGATC
GGTTCAACGC
CTCCCTGGGC
ACATACCACG
ATCTGCTGAA
AATTATCAAG
GACAAGGACT
TCCTGGACAA
TGAGGAAAAC
GAGGACATTC
TGGAAGATAT
CGTGCTGACC
CTGACACTGT
TTGAGGACAG
AGAGATGATC
GAGGAACGGC
TGAAAACCTA
TGCCCACCTG
TTCGACGACA
AAGTGATGAA
GCAGCTGAAG
CGGCGGAGAT
ACACCGGCTG
GGGCAGGCTG
AGCCGGAAGC
TGATCAACGG
CATCCGGGAC
AAGCAGTCCG
GCAAGACAAT
CCTGGATTTC
CTGAAGTCCG
ACGGCTTCGC
CAACAGAAAC
TTCATGCAGC
TGATCCACGA
CGACAGCCTG
ACCTTTAAAG
AGGACATCCA
GAAAGCCCAG
GTGTCCGGCC
AGGGCGATAG
CCTGCACGAG
CACATTGCCA
ATCTGGCCGG
CAGCCCCGCC
ATTAAGAAGG
GCATCCTGCA
GACAGTGAAG
GTGGTGGACG
AGCTCGTGAA
AGTGATGGGC
CGGCACAAGC
CCGAGAACAT
CGTGATCGAA
ATGGCCAGAG
AGAACCAGAC
CACCCAGAAG
GGACAGAAGA
ACAGCCGCGA
GAGAATGAAG
CGGATCGAAG
AGGGCATCAA
AGAGCTGGGC
AGCCAGATCC
TGAAAGAACA
CCCCGTGGAA
AACACCCAGC
TGCAGAACGA
GAAGCTGTAC
CTGTACTACC
TGCAGAATGG
GCGGGATATG
TACGTGGACC
AGGAACTGGA
CATCAACCGG
CTGTCCGACT
ACGATGTGGA
CCATATCGTG
CCTCAGAGCT
TTCTGAAGGA
CGACTCCATC
GACAACAAGG
TGCTGACCAG
AAGCGACAAG
AACCGGGGCA
AGAGCGACAA
CGTGCCCTCC
GAAGAGGTCG
TGAAGAAGAT
GAAGAACTAC
TGGCGGCAGC
TGCTGAACGC
CAAGCTGATT
ACCCAGAGAA
AGTTCGACAA
TCTGACCAAG
GCCGAGAGAG
GCGGCCTGAG
CGAACTGGAT
AAGGCCGGCT
TCATCAAGAG
ACAGCTGGTG
GAAACCCGGC
AGATCACAAA
GCACGTGGCA
CAGATCCTGG
ACTCCCGGAT
GAACACTAAG
TACGACGAGA
ATGACAAGCT
GATCCGGGAA
GTGAAAGTGA
TCACCCTGAA
GTCCAAGCTG
GTGTCCGATT
TCCGGAAGGA
TTTCCAGTTT
TACAAAGTGC
GCGAGATCAA
CAACTACCAC
CACGCCCACG
ACGCCTACCT
GAACGCCGTC
GTGGGAACCG
CCCTGATCAA
AAAGTACCCT
AAGCTGGAAA
GCGAGTTCGT
GTACGGCGAC
TACAAGGTGT
ACGACGTGCG
GAAGATGATC
GCCAAGAGCG
AGCAGGAAAT
CGGCAAGGCT
ACCGCCAAGT
ACTTCTTCTA
CAGCAACATC
ATGAACTTTT
TCAAGACCGA
GATTACCCTG
GCCAACGGCG
AGATCCGGAA
GCGGCCTCTG
ATCGAGACAA
ACGGCGAAAC
CGGGGAGATC
GTGTGGGATA
AGGGCCGGGA
TTTTGCCACC
GTGCGGAAAG
TGCTGAGCAT
GCCCCAAGTG
AATATCGTGA
AAAAGACCGA
GGTGCAGACA
GGCGGCTTCA
GCAAAGAGTC
TATCCTGCCC
AAGAGGAACA
GCGATAAGCT
GATCGCCAGA
AAGAAGGACT
GGGACCCTAA
GAAGTACGGC
GGCTTCGACA
GCCCCACCGT
GGCCTATTCT
GTGCTGGTGG
TGGCCAAAGT
GGAAAAGGGC
AAGTCCAAGA
AACTGAAGAG
TGTGAAAGAG
CTGCTGGGGA
TCACCATCAT
GGAAAGAAGC
AGCTTCGAGA
AGAATCCCAT
CGACTTTCTG
GAAGCCAAGG
GCTACAAAGA
AGTGAAAAAG
GACCTGATCA
TCAAGCTGCC
TAAGTACTCC
CTGTTCGAGC
TGGAAAACGG
CCGGAAGAGA
ATGCTGGCCT
CTGCCGGCGA
ACTGCAGAAG
GGAAACGAAC
TGGCCCTGCC
CTCCAAATAT
GTGAACTTCC
TGTACCTGGC
CAGCCACTAT
GAGAAGCTGA
AGGGCTCCCC
CGAGGATAAT
GAGCAGAAAC
AGCTGTTTGT
GGAACAGCAC
AAGCACTACC
TGGACGAGAT
CATCGAGCAG
ATCAGCGAGT
TCTCCAAGAG
AGTGATCCTG
GCCGACGCTA
ATCTGGACAA
AGTGCTGTCC
GCCTACAACA
AGCACCGGGA
TAAGCCCATC
AGAGAGCAGG
CCGAGAATAT
CATCCACCTG
TTTACCCTGA
CCAATCTGGG
AGCCCCTGCC
GCCTTCAAGT
ACTTTGACAC
CACCATCGAC
CGGAAGAGGT
ACACCAGCAC
CAAAGAGGTG
CTGGACGCCA
CCCTGATCCA
CCAGAGCATC
ACCGGCCTGT
ACGAGACACG
GATCGACCTG
TCTCAGCTGG
GAGGCGACAA
GCGACCTGCC
GCCACAAAGA
AGGCTGGACA
GGCTAAGAAG
AAGAAAGATT
ACAAAGACGA
TGACGATAAG
GGATCCGGCG
CAACAAACTT
CTCTCTGCTG
AAACAAGCCG
GAGATGTCGA
AGAGAATCCT
GGACCGATTG
AACAAGATGG
ATTGCACGCA
GGTTCTCCGG
CCGCTTGGGT
GGAGAGGCTA
TTCGGCTATG
ACTGGGCACA
ACAGACAATC
GGCTGCTCTG
ATGCCGCCGT
GTTCCGGCTG
TCAGCGCAGG
GGCGCCCGGT
TCTTTTTGTC
AAGACCGACC
TGTCCGGTGC
CCTGAATGAA
CTGCAGGACG
AGGCAGCGCG
GCTATCGTGG
CTGGCCACGA
CGGGCGTTCC
TTGCGCAGCT
GTGCTCGACG
TTGTCACTGA
AGCGGGAAGG
GACTGGCTGC
TATTGGGCGA
AGTGCCGGGG
CAGGATCTCC
TGTCATCTCA
CCTTGCTCCT
GCCGAGAAAG
TATCCATCAT
GGCTGATGCA
ATGCGGCGGC
TGCATACGCT
TGATCCGGCT
ACCTGCCCAT
TCGACCACCA
AGCGAAACAT
CGCATCGAGC
GAGCACGTAC
TCGGATGGAA
GCCGGTCTTG
TCGATCAGGA
TGATCTGGAC
GAAGAGCATC
AGGGGCTCGC
GCCAGCCGAA
CTGTTCGCCA
GGCTCAAGGC
GCGCATGCCC
GACGGCGAGG
ATCTCGTCGT
GACCCATGGC
GATGCCTGCT
TGCCGAATAT
CATGGTGGAA
AATGGCCGCT
TTTCTGGATT
CATCGACTGT
GGCCGGCTGG
GTGTGGCGGA
CCGCTATCAG
GACATAGCGT
TGGCTACCCG
TGATATTGCT
GAAGAGCTTG
GCGGCGAATG
GGCTGACCGC
TTCCTCGTGC
TTTACGGTAT
CGCCGCTCCC
GATTCGCAGC
GCATCGCCTT
CTATCGCCTT
CTTGACGAGT
TCTTCTGAAC
GCGTTAAGTC
GACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCGTCGACTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGGG
CCCGTTTAAA
CCCGCTGATC
AGCCTCGACT
GTGCCTTCTA
GTTGCCAGCC
ATCTGTTGTT
TGCCCCTCCC
CCGTGCCTTC
CTTGACCCTG
GAAGGTGCCA
CTCCCACTGT
CCTTTCCTAA
TAAAATGAGG
AAATTGCATC
GCATTGTCTG
AGTAGGTGTC
ATTCTATTCT
GGGGGGTGGG
GTGGGGCAGG
ACAGCAAGGG
GGAGGATTGG
GAAGACAATA
GCAGGCATGC
TGGGGATGCG
GTGGGCTCTA
TGGCTTCTGA
GGCGGAAAGA
ACCAGCTGGG
GCTCTAGGGG
GTATCCCCAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCCTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTAGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
TCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGC
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTAAT
TCTGTGGAAT
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCT
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGG
CTTTTGCAAA
AAGCTCCCGG
GAGCTTGTAT
ATCCATTTTC
GGATCTGATC
AGCACGTGTT
GACAATTAAT
CATCGGCATA
GTATATCGGC
ATAGTATAAT
ACGACAAGGT
GAGGAACTAA
ACCATGGCCA
AGTTGACCAG
TGCCGTTCCG
GTGCTCACCG
CGCGCGACGT
CGCCGGAGCG
GTCGAGTTCT
GGACCGACCG
GCTCGGGTTC
TCCCGGGACT
TCGTGGAGGA
CGACTTCGCC
GGTGTGGTCC
GGGACGACGT
GACCCTGTTC
ATCAGCGCGG
TCCAGGACCA
GGTGGTGCCG
GACAACACCC
TGGCCTGGGT
GTGGGTGCGC
GGCCTGGACG
AGCTGTACGC
CGAGTGGTCG
GAGGTCGTGT
CCACGAACTT
CCGGGACGCC
TCCGGGCCGG
CCATGACCGA
GATCGGCGAG
CAGCCGTGGG
GGCGGGAGTT
CGCCCTGCGC
GACCCGGCCG
GCAACTGCGT
GCACTTCGTG
GCCGAGGAGC
AGGACTGACA
CGTGCTACGA
GATTTCGATT
CCACCGCCGC
CTTCTATGAA
AGGTTGGGCT
TCGGAATCGT
TTTCCGGGAC
GCCGGCTGGA
TGATCCTCCA
GCGCGGGGAT
CTCATGCTGG
AGTTCTTCGC
CCACCCCAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGTA
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
AACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
AC