名称: | pLV3-CMV-AARS1(human)-3×HA-Neo |
---|---|
别称: | NM_001605.3;AARS; CMT2N; DEE29; EIEE29; HDLS2; TTD8 |
基因长: | 2904bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pCDH-CMV-MCS-EF1-Neo |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-AARS1(human)-3×HA-Neo是适用于哺乳细胞过表达质粒。质粒带有的CMV强启动子可启动下游基因转录,促进基因C端融合表达3×HA标签,便于操作蛋白免疫沉淀、蛋白共免疫沉淀等实验。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带Neo/G418抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCTAGCG
AATTCGCCAC
CATGGACTCT
ACTCTAACAG
CAAGTGAAAT
CCGGCAGCGA
TTTATAGATT
TCTTCAAGAG
GAACGAGCAT
ACGTATGTTC
ACTCGTCTGC
CACCATCCCA
TTGGATGACC
CCACTTTGCT
CTTTGCCAAT
GCAGGCATGA
ACCAGTTTAA
ACCCATTTTC
CTGAACACAA
TTGACCCATC
TCACCCCATG
GCAAAGCTGA
GCAGAGCTGC
CAATACCCAG
AAGTGCATCC
GGGCTGGGGG
CAAACATAAT
GACCTGGACG
ATGTGGGCAA
GGATGTCTAT
CATCACACCT
TCTTCGAGAT
GCTGGGCTCT
TGGTCTTTTG
GAGATTACTT
TAAGGAATTG
GCATGTAAGA
TGGCTCTGGA
ACTCCTCACC
CAAGAGTTTG
GCATTCCCAT
TGAAAGACTT
TATGTTACTT
ACTTTGGCGG
GGATGAAGCA
GCTGGCTTAG
AAGCAGATCT
GGAATGCAAA
CAGATCTGGC
AAAATTTGGG
GCTGGATGAC
ACCAAAATCC
TCCCAGGCAA
CATGAAGGAT
AACTTCTGGG
AGATGGGTGA
CACGGGCCCC
TGTGGTCCTT
GCAGTGAGAT
CCACTACGAC
CGGATTGGTG
GTCGGGACGC
CGCACATCTT
GTCAACCAGG
ACGACCCTAA
TGTGCTGGAG
ATCTGGAACC
TTGTGTTCAT
CCAGTATAAC
AGGGAAGCTG
ATGGCATTCT
GAAACCTCTT
CCCAAGAAAA
GCATTGACAC
AGGGATGGGC
CTGGAACGAC
TGGTATCTGT
GCTGCAGAAT
AAGATGTCCA
ACTATGACAC
TGACCTTTTT
GTCCCTTACT
TTGAAGCCAT
TCAGAAGGGC
ACAGGTGCCC
GACCATACAC
TGGGAAAGTT
GGTGCTGAGG
ATGCCGATGG
GATTGACATG
GCCTACCGGG
TGCTGGCTGA
CCACGCTCGG
ACCATCACTG
TGGCACTGGC
TGATGGTGGC
CGGCCTGACA
ACACAGGGCG
TGGATATGTG
TTGAGACGGA
TTCTCCGCCG
AGCTGTCCGA
TACGCCCATG
AAAAGCTCAA
TGCCAGCAGG
GGCTTCTTTG
CTACGTTAGT
GGATGTTGTC
GTCCAGTCCC
TGGGAGATGC
ATTTCCTGAG
CTGAAGAAGG
ACCCAGACAT
GGTGAAGGAC
ATCATTAATG
AAGAAGAGGT
GCAGTTTCTC
AAGACTCTCA
GCAGAGGGCG
TCGCATCCTG
GACAGGAAAA
TTCAGAGCCT
GGGAGACAGC
AAGACCATTC
CCGGAGACAC
TGCTTGGCTC
CTCTATGACA
CCTATGGGTT
TCCAGTGGAT
CTGACTGGAC
TGATTGCTGA
AGAGAAGGGC
CTGGTGGTAG
ACATGGATGG
CTTTGAAGAG
GAGAGGAAAC
TGGCCCAGCT
GAAATCACAG
GGCAAGGGAG
CTGGTGGGGA
AGACCTCATT
ATGCTGGACA
TTTACGCTAT
CGAAGAGCTC
CGGGCACGGG
GTCTGGAGGT
CACAGATGAT
TCCCCAAAGT
ACAATTACCA
TTTGGACTCC
AGTGGTAGCT
ATGTATTTGA
GAACACAGTG
GCTACGGTGA
TGGCTCTGCG
CAGGGAGAAG
ATGTTCGTGG
AAGAGGTGTC
CACAGGCCAG
GAGTGTGGAG
TGGTGCTGGA
CAAGACCTGT
TTCTATGCTG
AGCAAGGAGG
CCAGATCTAT
GACGAAGGCT
ACCTGGTGAA
GGTGGATGAC
AGCAGTGAAG
ATAAAACAGA
GTTTACAGTG
AAGAATGCTC
AGGTCCGAGG
AGGGTATGTG
CTACACATTG
GAACCATCTA
CGGTGACCTG
AAAGTGGGGG
ATCAGGTCTG
GCTGTTTATT
GATGAGCCCC
GACGAAGACC
CATCATGAGC
AACCACACAG
CTACGCACAT
TCTGAACTTC
GCCCTGCGCT
CAGTGCTTGG
GGAAGCTGAC
CAGAAAGGCT
CATTGGTTGC
TCCTGACCGC
CTCAGATTTG
ACTTTACTGC
CAAGGGAGCC
ATGTCCACCC
AACAGATCAA
GAAGGCTGAA
GAGATTGCTA
ATGAGATGAT
TGAGGCAGCC
AAGGCCGTCT
ATACCCAGGA
TTGCCCCCTG
GCAGCAGCGA
AAGCCATCCA
GGGCCTACGG
GCTGTGTTTG
ATGAGACCTA
TCCTGACCCT
GTGCGAGTCG
TCTCCATTGG
GGTCCCGGTG
TCCGAGTTGC
TGGATGACCC
CTCTGGGCCT
GCTGGCTCCC
TGACTTCTGT
TGAGTTCTGT
GGGGGAACGC
ACCTGCGGAA
CTCGAGTCAT
GCAGGAGCTT
TTGTGATCGT
GACGGAAGAA
GCCATTGCCA
AGGGTATCCG
GAGGATTGTG
GCTGTCACAG
GTGCCGAGGC
CCAGAAGGCC
CTCAGGAAAG
CAGAGAGCTT
GAAGAAATGT
CTCTCTGTCA
TGGAAGCCAA
AGTGAAGGCT
CAGACTGCTC
CAAACAAGGA
TGTGCAGAGG
GAGATCGCTG
ACCTTGGAGA
GGCCCTGGCC
ACTGCAGTCA
TCCCCCAGTG
GCAGAAGGAT
GAATTGCGGG
AGACTCTCAA
ATCCCTAAAG
AAGGTCATGG
ATGACTTGGA
CCGAGCCAGC
AAAGCCGATG
TCCAGAAACG
AGTGTTAGAG
AAGACGAAGC
AGTTCATCGA
CAGCAACCCC
AACCAGCCTC
TTGTCATCCT
GGAGATGGAG
AGCGGCGCCT
CAGCCAAGGC
CCTGAATGAA
GCCTTGAAGC
TCTTCAAGAT
GCACTCCCCT
CAGACTTCTG
CCATGCTCTT
CACGGTGGAC
AATGAGGCTG
GCAAGATCAC
GTGCCTGTGT
CAAGTTCCCC
AGAATGCAGC
CAATCGGGGC
TTAAAAGCCA
GCGAGTGGGT
GCAGCAGGTG
TCAGGCTTGA
TGGACGGTAA
AGGTGGTGGC
AAGGATGTGT
CTGCACAGGC
CACAGGCAAG
AACGTTGGCT
GCCTGCAGGA
GGCGCTGCAG
CTGGCCACTT
CCTTCGCCCA
GCTGCGCCTC
GGGGATGTAA
AGAACCTCGA
GTACCCATAC
GACGTACCTG
ATTATGCAAC
TTATCCTTAT
GATGTACCTG
ATTATGCTAG
CTATCCTTAT
GATGTCCCAG
ACTACGCATA
GGATCCGCGG
CCGCGAAGGA
TCTGCGATCG
CTCCGGTGCC
CGTCAGTGGG
CAGAGCGCAC
ATCGCCCACA
GTCCCCGAGA
AGTTGGGGGG
AGGGGTCGGC
AATTGAACGG
GTGCCTAGAG
AAGGTGGCGC
GGGGTAAACT
GGGAAAGTGA
TGTCGTGTAC
TGGCTCCGCC
TTTTTCCCGA
GGGTGGGGGA
GAACCGTATA
TAAGTGCAGT
AGTCGCCGTG
AACGTTCTTT
TTCGCAACGG
GTTTGCCGCC
AGAACACAGC
TGAAGCTTCG
AGGGGCTCGC
ATCTCTCCTT
CACGCGCCCG
CCGCCCTACC
TGAGGCCGCC
ATCCACGCCG
GTTGAGTCGC
GTTCTGCCGC
CTCCCGCCTG
TGGTGCCTCC
TGAACTGCGT
CCGCCGTCTA
GGTAAGTTTA
AAGCTCAGGT
CGAGACCGGG
CCTTTGTCCG
GCGCTCCCTT
GGAGCCTACC
TAGACTCAGC
CGGCTCTCCA
CGCTTTGCCT
GACCCTGCTT
GCTCAACTCT
ACGTCTTTGT
TTCGTTTTCT
GTTCTGCGCC
GTTACAGATC
CAAGCTGTGA
CCGGCGCCTA
CGCTAGGCCA
CCATGGCTGA
ACAAGATGGA
TTGCACGCAG
GTTCTCCGGC
CGCTTGGGTG
GAGAGGCTAT
TCGGCTATGA
CTGGGCACAA
CAGACAATCG
GCTGCTCTGA
TGCCGCCGTG
TTCCGGCTGT
CAGCGCAGGG
GCGCCCGGTT
CTTTTTGTCA
AGACCGACCT
GTCCGGTGCC
CTGAATGAAC
TGCAGGACGA
GGCAGCGCGG
CTATCGTGGC
TGGCCACGAC
GGGCGTTCCT
TGCGCAGCTG
TGCTCGACGT
TGTCACTGAA
GCGGGAAGGG
ACTGGCTGCT
ATTGGGCGAA
GTGCCGGGGC
AGGATCTCCT
GTCATCTCAC
CTTGCTCCTG
CCGAGAAAGT
ATCCATCATG
GCTGATGCAA
TGCGGCGGCT
GCATACGCTT
GATCCGGCTA
CCTGCCCATT
CGACCACCAA
GCGAAACATC
GCATCGAGCG
AGCACGTACT
CGGATGGAAG
CCGGTCTTGT
CGATCAGGAT
GATCTGGACG
AAGAGCATCA
GGGGCTCGCG
CCAGCCGAAC
TGTTCGCCAG
GCTCAAGGCG
CGCATGCCCG
ACGGCGAGGA
TCTCGTCGTG
ACCCATGGCG
ATGCCTGCTT
GCCGAATATC
ATGGTGGAAA
ATGGCCGCTT
TTCTGGATTC
ATCGACTGTG
GCCGGCTGGG
TGTGGCGGAC
CGCTATCAGG
ACATAGCGTT
GGCTACCCGT
GATATTGCTG
AAGAGCTTGG
CGGCGAATGG
GCTGACCGCT
TCCTCGTGCT
TTACGGTATC
GCCGCTCCCG
ATTCGCAGCG
CATCGCCTTC
TATCGCCTTC
TTGACGAGTT
CTTCTGAGTC
GACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCCTGGTACC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAAATAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TAGTAGTTCA
TGTCATCTTA
TTATTCAGTA
TTTATAACTT
GCAAAGAAAT
GAATATCAGA
GAGTGAGAGG
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGCTCTAGCT
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCG
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGA
CTTTTGCAGA
GACGGCCCAA
ATTCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCATACTCT
TCCTTTTTCA
ATATTATTGA
AGCATTTATC
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGACGT
CTAAGAAACC
ATTATTATCA
TGACATTAAC
CTATAAAAAT
AGGCGTATCA
CGAGGCCCTT
TCGTCTCGCG
CGTTTCGGTG
ATGACGGTGA
AAACCTCTGA
CACATGCAGC
TCCCGGAGAC
GGTCACAGCT
TGTCTGTAAG
CGGATGCCGG
GAGCAGACAA
GCCCGTCAGG
GCGCGTCAGC
GGGTGTTGGC
GGGTGTCGGG
GCTGGCTTAA
CTATGCGGCA
TCAGAGCAGA
TTGTACTGAG
AGTGCACCAT
ATGCGGTGTG
AAATACCGCA
CAGATGCGTA
AGGAGAAAAT
ACCGCATCAG
GCGCCATTCG
CCATTCAGGC
TGCGCAACTG
TTGGGAAGGG
CGATCGGTGC
GGGCCTCTTC
GCTATTACGC
CAGCTGGCGA
AAGGGGGATG
TGCTGCAAGG
CGATTAAGTT
GGGTAACGCC
AGGGTTTTCC
CAGTCACGAC
GTTGTAAAAC
GACGGCCAGT
GCCAAGCTG