名称: | pLV3-EF1a-CD19-CAR-P2A-ITGA8(human)-3×Myc-Puro |
---|---|
别称: | - |
基因长: | 3189bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-EF1a-CD19-CAR-P2A-ITGA8(human)-PGK-Puro |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-EF1a-CD19-CAR-P2A-ITGA8(human)-3×Myc-Puro是适用于哺乳细胞过表达的质粒。质粒带有的EF1a启动子可启动下游的CD19-CAR基因及人源的ITGA8基因转录,促进ITGA8基因C端融合表达3×Myc标签,便于融合蛋白与镍离子发生亲和作用进行蛋白纯化,也可使用抗Myc抗体来检测和识别目的蛋白。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTTAACTTT
TAAAAGAAAA
GGGGGGATTG
GGGGGTACAG
TGCAGGGGAA
AGAATAGTAG
ACATAATAGC
AACAGACATA
CAAACTAAAG
AATTACAAAA
ACAAATTACA
AAAATTCAAA
ATTTTATCGA
TACTAGTAAG
GATCTGCGAT
CGCTCCGGTG
CCCGTCAGTG
GGCAGAGCGC
ACATCGCCCA
CAGTCCCCGA
GAAGTTGGGG
GGAGGGGTCG
GCAATTGAAC
GGGTGCCTAG
AGAAGGTGGC
GCGGGGTAAA
CTGGGAAAGT
GATGTCGTGT
ACTGGCTCCG
CCTTTTTCCC
GAGGGTGGGG
GAGAACCGTA
TATAAGTGCA
GTAGTCGCCG
TGAACGTTCT
TTTTCGCAAC
GGGTTTGCCG
CCAGAACACA
GCTGAAGCTT
CGAGGGGCTC
GCATCTCTCC
TTCACGCGCC
CGCCGCCCTA
CCTGAGGCCG
CCATCCACGC
CGGTTGAGTC
GCGTTCTGCC
GCCTCCCGCC
TGTGGTGCCT
CCTGAACTGC
GTCCGCCGTC
TAGGTAAGTT
TAAAGCTCAG
GTCGAGACCG
GGCCTTTGTC
CGGCGCTCCC
TTGGAGCCTA
CCTAGACTCA
GCCGGCTCTC
CACGCTTTGC
CTGACCCTGC
TTGCTCAACT
CTACGTCTTT
GTTTCGTTTT
CTGTTCTGCG
CCGTTACAGA
TCCAAGCTGT
GACCGGCGCC
TACTCTAGAA
TGGCTCTGCC
AGTGACAGCT
CTGCTGCTTC
CACTGGCACT
GTTGCTGCAT
GCCGCAAGGC
CTGACTACAA
AGATGACGAC
GACAAGGGAG
GTGGCGGATC
TGAGATCGTC
TTGACACAGT
CACCTGCCAC
CTTGTCTCTG
TCACCTGGCG
AGAGAGCCAC
CTTGAGCTGT
AGAGCCAGCC
AAGACATCAG
CAAGTATCTG
AACTGGTATC
AGCAGAAGCC
AGGTCAGGCA
CCTAGACTGC
TGATCTACCA
CACCTCTCGG
CTTCACTCCG
GCATTCCAGA
TCGGTTCTCT
GGTTCCGGCA
GCGGCACAGA
CTTCACTCTG
ACCATCAGCA
GGCTGGAGCC
AGAGGACATC
GCCGTGTACT
ACTGTCAACA
GGGCAACACA
CTGCCATACA
CCTTCGGTCA
GGGAACCAAG
CTGGAAATCG
GTGGCGGCGG
AAGCGGCGGA
GGTGGATCTG
GAGGCGGTGG
AAGCCAAGTC
ACATTGAGAG
AAAGCGGTCC
AGCTCTGGTG
AAGCCTACTC
AGACCTTGAC
CTTGACATGC
ACCTTCTCCG
GCGTTAGCCT
GCCTGACTAT
GGAGTGAGCT
GGATCAGACA
GCCACCTGGT
AAGGCTCTGG
AATGGCTCGC
CGTGATCTGG
GGCAGCGAGA
CAACCTACTA
CAACAGCGCA
CTGAAGTCCA
GACTGACCAT
CTCCAAAGAC
ACCTCCAAGA
ACCAGGTGGT
GTTGACCATG
ACAAACATGG
ACCCAGTGGA
CACCGCTACC
TACTATTGTG
CCAAGCACTA
CTACTACGGT
GGCAGTTACG
CCATGGATTA
CTGGGGTCAG
GGCACCACTG
TCACCGTGTC
CAGCACCACC
ACTCCAGCAC
CTAGGCCTCC
AACCCCAGCT
CCAACAATCG
CCAGTCAGCC
ACTGTCTCTT
AGACCTGAGG
CCTGCAGACC
AGCAGCAGGT
GGTGCCGTGC
ATACCAGAGG
CTTGGACTTC
GCCTGTGACA
TCTACATCTG
GGCTCCATTG
GCTGGCACAT
GCGGCGTACT
GCTGCTGAGC
CTGGTCATCA
CACTGTACTG
CAAGCGCGGT
CGGAAGAAGT
TGCTGTACAT
CTTCAAGCAG
CCATTTATGC
GGCCTGTCCA
GACCACACAA
GAAGAAGATG
GATGCAGCTG
CAGATTTCCA
GAAGAAGAAG
AGGGCGGTTG
TGAGTTGCGG
GTCAAGTTCT
CCAGATCTGC
TGACGCTCCA
GCTTACCAGC
AGGGCCAGAA
TCAGCTGTAT
AACGAGTTGA
ACCTGGGCAG
AAGGGAAGAA
TACGATGTGC
TGGATAAGAG
GCGCGGAAGG
GACCCTGAGA
TGGGTGGTAA
GCCTAGACGG
AAGAATCCAC
AAGAGGGTCT
GTACAATGAG
CTGCAGAAAG
ATAAGATGGC
CGAGGCCTAC
TCCGAGATCG
GCATGAAGGG
CGAGAGACGG
AGAGGCAAGG
GCCACGATGG
TCTGTACCAG
GGCTTGAGCA
CCGCTACTAA
AGACACTTAC
GACGCTCTGC
ACATGCAGGC
ACTGCCTCCA
AGAGCCACGA
ACTTCTCTCT
GTTAAAGCAA
GCAGGAGATG
TTGAAGAAAA
CCCCGGGCCT
GCTAGCGAAT
TCGCCACCAT
GTCGCCCGGG
GCCAGCCGCG
GTCCCCGGGG
AAGCCAGGCG
CCGCTGATCG
CGCCCCTCTG
CTGCGCCGCG
GCCGCGCTGG
GGATGTTGCT
GTGGTCCCCC
GCCTGTCAGG
CGTTCAACCT
GGACGTGGAA
AAGCTCACAG
TGTACAGCGG
CCCCAAGGGC
AGCTACTTCG
GCTACGCCGT
GGACTTCCAC
ATACCCGACG
CCCGCACAGC
GAGTGTCTTG
GTGGGGGCGC
CCAAAGCCAA
CACCAGCCAG
CCCGATATCG
TGGAAGGGGG
AGCCGTCTAT
TACTGTCCTT
GGCCCGCGGA
GGGGTCTGCG
CAGTGCAGGC
AGATACCGTT
TGACACCACC
AACAACAGAA
AGATCAGAGT
TAATGGAACC
AAAGAACCTA
TCGAGTTCAA
ATCCAATCAG
TGGTTTGGAG
CAACAGTGAA
AGCTCACAAA
GGAAAAGTTG
TGGCCTGTGC
TCCTTTATAT
CACTGGAGAA
CTCTTAAACC
GACACCAGAA
AAGGACCCAG
TTGGCACCTG
CTATGTAGCA
ATTCAGAACT
TCAGCGCCTA
TGCCGAGTTC
TCTCCTTGCC
GGAACAGCAA
TGCTGATCCG
GAAGGCCAGG
GTTACTGCCA
AGCAGGATTT
AGTCTGGATT
TTTATAAGAA
TGGAGACCTT
ATTGTGGGAG
GACCTGGGAG
TTTCTACTGG
CAAGGACAAG
TGATCACTGC
CAGTGTTGCA
GATATCATTG
CAAATTACTC
ATTCAAGGAT
ATCCTCAGGA
AACTGGCAGG
AGAAAAGCAG
ACGGAAGTGG
CTCCAGCTTC
CTATGATGAC
AGTTACCTTG
GATACTCAGT
TGCTGCTGGG
GAGTTTACTG
GGGATTCTCA
GCAAGAATTG
GTTGCTGGAA
TTCCAAGAGG
AGCACAGAAT
TTTGGATATG
TTTCCATCAT
TAACTCTACG
GATATGACGT
TTATTCAGAA
TTTCACGGGA
GAACAGATGG
CATCTTATTT
TGGATATACC
GTTGTCGTAT
CAGATGTTAA
CAGTGATGGA
CTGGATGATG
TCCTGGTTGG
GGCACCTCTC
TTTATGGAAC
GTGAATTTGA
GAGCAACCCC
AGAGAAGTAG
GGCAAATCTA
CCTGTATTTG
CAAGTGAGCT
CTCTCCTCTT
CAGAGACCCC
CAGATCCTCA
CTGGCACCGA
GACGTTTGGG
AGATTCGGTA
GTGCTATGGC
ACACTTAGGA
GACCTGAACC
AAGATGGATA
CAATGACATT
GCCATCGGAG
TGCCTTTTGC
AGGCAAGGAT
CAAAGAGGCA
AAGTGCTCAT
TTATAATGGG
AACAAAGATG
GCTTAAACAC
CAAGCCTTCC
CAAGTTCTGC
AAGGAGTGTG
GGCCTCACAT
GCTGTCCCTT
CCGGATTTGG
CTTTACTTTA
AGAGGAGATT
CAGACATAGA
CAAGAATGAT
TACCCAGATT
TGATTGTGGG
TGCATTTGGA
ACAGGAAAAG
TCGCTGTTTA
CAGAGCAAGA
CCGGTTGTGA
CTGTAGATGC
CCAGCTTCTG
CTGCACCCAA
TGATTATCAA
TCTTGAAAAT
AAAACTTGCC
AGGTTCCAGA
CTCTATGACA
TCTGCTGCCT
GCTTTTCTTT
AAGAGTATGT
GCATCTGTCA
CAGGCCAGAG
CATTGCAAAC
ACAATAGTCT
TGATGGCAGA
GGTGCAATTA
GATTCCCTGA
AACAGAAAGG
AGCTATTAAA
CGGACGCTCT
TCCTTGATAA
CCATCAGGCT
CATCGCGTCT
TCCCTCTTGT
GATAAAAAGG
CAGAAATCCC
ACCAGTGCCA
GGATTTCATC
GTTTACCTTC
GAGATGAAAC
TGAATTCCGA
GATAAATTAT
CTCCAATCAA
CATTAGTTTG
AATTACAGTT
TGGACGAATC
CACCTTTAAA
GAAGGCCTGG
AAGTGAAACC
AATATTGAAC
TACTACAGAG
AAAACATTGT
TAGTGAACAG
GCTCACATTC
TGGTGGACTG
TGGAGAAGAC
AATCTGTGTG
TTCCTGACTT
GAAGCTGTCG
GCTAGACCAG
ATAAGCATCA
GGTAATCATT
GGAGATGAAA
ATCACCTTAT
GCTCATAATA
AATGCAAGAA
ATGAAGGGGA
AGGAGCATAT
GAAGCTGAAC
TCTTTGTAAT
GATACCAGAA
GAGGCAGATT
ATGTTGGAAT
CGAACGCAAC
AACAAGGGAT
TTCGACCACT
GAGCTGTGAG
TACAAGATGG
AAAATGTAAC
CAGGATGGTG
GTGTGTGACC
TTGGGAACCC
TATGGTGTCT
GGAACAAATT
ATTCCCTGGG
CCTCCGATTT
GCAGTTCCAC
GTCTTGAGAA
AACAAACATG
AGCATTAACT
TCGATCTCCA
AATCAGAAGT
TCCAACAAGG
ACAATCCAGA
CAGCAATTTT
GTGAGCCTGC
AAATCAACAT
CACTGCTGTA
GCGCAGGTGG
AAATAAGAGG
AGTGTCACAC
CCTCCGCAGA
TTGTTCTGCC
CATTCATAAC
TGGGAACCAG
AAGAGGAGCC
CCACAAAGAG
GAGGAGGTTG
GACCATTGGT
GGAACATATT
TATGAGCTGC
ACAATATTGG
ACCAAGTACC
ATCAGTGACA
CCATCCTGGA
GGTGGGCTGG
CCTTTCTCTG
CCCGGGATGA
ATTTCTTCTC
TATATTTTCC
ATATTCAAAC
TCTGGGACCT
CTGCAGTGCC
AACCAAATCC
TAATATCAAT
CCACAGGATA
TAAAGCCTGC
TGCCTCCCCA
GAGGACACCC
CTGAGCTCAG
CGCCTTTTTG
CGAAACTCTA
CTATTCCTCA
TCTTGTCAGG
AAGAGGGATG
TACATGTGGT
CGAATTCCAC
AGACAGAGCC
CTGCAAAAAT
ACTGAATTGT
ACAAATATCG
AGTGTTTACA
AATCTCCTGT
GCAGTGGGAC
GACTCGAAGG
AGGAGAAAGC
GCAGTCCTGA
AAGTCAGGTC
ACGATTATGG
GCCCACACCT
TCCTCCAGAG
AAAAAATGAT
CCCTATGCTC
TTGCATCCCT
GGTGTCCTTT
GAAGTTAAGA
AGATGCCTTA
TACAGATCAG
CCAGCAAAAC
TCCCAGAAGG
AAGCATAGTA
ATTAAGACAT
CAGTTATTTG
GGCAACTCCG
AATGTTTCCT
TCTCAATCCC
ATTATGGGTA
ATAATACTAG
CAATACTTCT
TGGATTGTTG
GTTCTCGCCA
TTTTAACCTT
AGCTTTATGG
AAGTGTGGAT
TCTTTGACAG
AGCCAGACCT
CCTCAGGAGG
ACATGACCGA
CAGGGAACAG
CTGACAAATG
ACAAGACCCC
TGAGGCACTC
GAGGAACAAA
AACTAATATC
AGAGGAAGAT
TTGGAACAGA
AACTAATCAG
TGAAGAAGAT
TTAGAGCAAA
AGTTAATTTC
CGAGGAGGAC
TTATAGGCGG
CCGCTGGGTA
GGGGAGGCGC
TTTTCCCAAG
GCAGTCTGGA
GCATGCGCTT
TAGCAGCCCC
GCTGGGCACT
TGGCGCTACA
CAAGTGGCCT
CTGGCCTCGC
ACACATTCCA
CATCCACCGG
TAGGCGCCAA
CCGGCTCCGT
TCTTTGGTGG
CCCCTTCGCG
CCACCTTCTA
CTCCTCCCCT
AGTCAGGAAG
TTCCCCCCCG
CCCCGCAGCT
CGCGTCGTGC
AGGACGTGAC
AAATGGAAGT
AGCACGTCTC
ACTAGTCTCG
TGCAGATGGA
CAGCACCGCT
GAGCAATGGA
AGCGGGTAGG
CCTTTGGGGC
AGCGGCCAAT
AGCAGCTTTG
CTCCTTCGCT
TTCTGGGCTC
AGAGGCTGGG
AAGGGGTGGG
TCCGGGGGCG
GGCTCAGGGG
CGGGCTCAGG
GGCGGGGCGG
GCGCCCGAAG
GTCCTCCGGA
GGCCCGGCAT
TCTGCACGCT
TCAAAAGCGC
ACGTCTGCCG
CGCTGTTCTC
CTCTTCCTCA
TCTCCGGGCC
TTTCGACCTG
CAGCCCAAGC
TTACCATGAC
CGAGTACAAG
CCCACGGTGC
GCCTCGCCAC
CCGCGACGAC
GTCCCCAGGG
CCGTACGCAC
CCTCGCCGCC
GCGTTCGCCG
ACTACCCCGC
CACGCGCCAC
ACCGTCGATC
CGGACCGCCA
CATCGAGCGG
GTCACCGAGC
TGCAAGAACT
CTTCCTCACG
CGCGTCGGGC
TCGACATCGG
CAAGGTGTGG
GTCGCGGACG
ACGGCGCCGC
GGTGGCGGTC
TGGACCACGC
CGGAGAGCGT
CGAAGCGGGG
GCGGTGTTCG
CCGAGATCGG
CCCGCGCATG
GCCGAGTTGA
GCGGTTCCCG
GCTGGCCGCG
CAGCAACAGA
TGGAAGGCCT
CCTGGCGCCG
CACCGGCCCA
AGGAGCCCGC
GTGGTTCCTG
GCCACCGTCG
GCGTCTCGCC
CGACCACCAG
GGCAAGGGTC
TGGGCAGCGC
CGTCGTGCTC
CCCGGAGTGG
AGGCGGCCGA
GCGCGCCGGG
GTGCCCGCCT
TCCTGGAGAC
CTCCGCGCCC
CGCAACCTCC
CCTTCTACGA
GCGGCTCGGC
TTCACCGTCA
CCGCCGACGT
CGAGGTGCCC
GAAGGACCGC
GCACCTGGTG
CATGACCCGC
AAGCCCGGTG
CCTGAGTCGA
CAATCAACCT
CTGGATTACA
AAATTTGTGA
AAGATTGACT
GGTATTCTTA
ACTATGTTGC
TCCTTTTACG
CTATGTGGAT
ACGCTGCTTT
AATGCCTTTG
TATCATGCTA
TTGCTTCCCG
TATGGCTTTC
ATTTTCTCCT
CCTTGTATAA
ATCCTGGTTG
CTGTCTCTTT
ATGAGGAGTT
GTGGCCCGTT
GTCAGGCAAC
GTGGCGTGGT
GTGCACTGTG
TTTGCTGACG
CAACCCCCAC
TGGTTGGGGC
ATTGCCACCA
CCTGTCAGCT
CCTTTCCGGG
ACTTTCGCTT
TCCCCCTCCC
TATTGCCACG
GCGGAACTCA
TCGCCGCCTG
CCTTGCCCGC
TGCTGGACAG
GGGCTCGGCT
GTTGGGCACT
GACAATTCCG
TGGTGTTGTC
GGGGAAATCA
TCGTCCTTTC
CTTGGCTGCT
CGCCTGTGTT
GCCACCTGGA
TTCTGCGCGG
GACGTCCTTC
TGCTACGTCC
CTTCGGCCCT
CAATCCAGCG
GACCTTCCTT
CCCGCGGCCT
GCTGCCGGCT
CTGCGGCCTC
TTCCGCGTCT
TCGCCTTCGC
CCTCAGACGA
GTCGGATCTC
CCTTTGGGCC
GCCTCCCCGC
CTGGTACCTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
AATAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTA
GTAGTTCATG
TCATCTTATT
ATTCAGTATT
TATAACTTGC
AAAGAAATGA
ATATCAGAGA
GTGAGAGGAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGG
CTCTAGCTAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGACT
TTTGCAGAGA
CGGCCCAAAT
TCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCCCTTTC
GTCTCGCGCG
TTTCGGTGAT
GACGGTGAAA
ACCTCTGACA
CATGCAGCTC
CCGGAGACGG
TCACAGCTTG
TCTGTAAGCG
GATGCCGGGA
GCAGACAAGC
CCGTCAGGGC
GCGTCAGCGG
GTGTTGGCGG
GTGTCGGGGC
TGGCTTAACT
ATGCGGCATC
AGAGCAGATT
GTACTGAGAG
TGCACCATAT
GCGGTGTGAA
ATACCGCACA
GATGCGTAAG
GAGAAAATAC
CGCATCAGGC
GCCATTCGCC
ATTCAGGCTG
CGCAACTGTT
GGGAAGGGCG
ATCGGTGCGG
GCCTCTTCGC
TATTACGCCA
GCTGGCGAAA
GGGGGATGTG
CTGCAAGGCG
ATTAAGTTGG
GTAACGCCAG
GGTTTTCCCA
GTCACGACGT
TGTAAAACGA
CGGCCAGTGC
CAAGCTG