名称: | pLV3-CMV-EGFP-RAB9A(human)-Puro |
---|---|
别称: | NM_004251.5;RAB9 |
基因长: | 606bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pCDH-CMV-EGFP-FOXQ1(human)-EF1a-Puro |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-EGFP-RAB9A(human)-Puro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动人源RAB9A基因转录。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带的EGFP绿色荧光蛋白可用于直观观察目的基因的表达和定位情况。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGACCGTCAG
ATCGCCTGGA
GACGCCATCC
ACGCTGTTTT
GACCTCCATA
GAAGATTCTA
GAGCTAGCGA
ATTCAGCCAC
CATGGTGAGC
AAGGGCGAGG
AGCTGTTCAC
CGGGGTGGTG
CCCATCCTGG
TCGAGCTGGA
CGGCGACGTA
AACGGCCACA
AGTTCAGCGT
GTCCGGCGAG
GGCGAGGGCG
ATGCCACCTA
CGGCAAGCTG
ACCCTGAAGT
TCATCTGCAC
CACCGGCAAG
CTGCCCGTGC
CCTGGCCCAC
CCTCGTGACC
ACCCTGACCT
ACGGCGTGCA
GTGCTTCAGC
CGCTACCCCG
ACCACATGAA
GCAGCACGAC
TTCTTCAAGT
CCGCCATGCC
CGAAGGCTAC
GTCCAGGAGC
GCACCATCTT
CTTCAAGGAC
GACGGCAACT
ACAAGACCCG
CGCCGAGGTG
AAGTTCGAGG
GCGACACCCT
GGTGAACCGC
ATCGAGCTGA
AGGGCATCGA
CTTCAAGGAG
GACGGCAACA
TCCTGGGGCA
CAAGCTGGAG
TACAACTACA
ACAGCCACAA
CGTCTATATC
ATGGCCGACA
AGCAGAAGAA
CGGCATCAAG
GTGAACTTCA
AGATCCGCCA
CAACATCGAG
GACGGCAGCG
TGCAGCTCGC
CGACCACTAC
CAGCAGAACA
CCCCCATCGG
CGACGGCCCC
GTGCTGCTGC
CCGACAACCA
CTACCTGAGC
ACCCAGTCCG
CCCTGAGCAA
AGACCCCAAC
GAGAAGCGCG
ATCACATGGT
CCTGCTGGAG
TTCGTGACCG
CCGCCGGGAT
CACTCTCGGC
ATGGACGAGC
TGTACAAGGG
AGGAGGGGGA
AGCGGAGGAG
GGGGAAGCAT
GGCAGGAAAA
TCATCACTTT
TTAAAGTAAT
TCTCCTTGGA
GATGGTGGAG
TTGGGAAGAG
TTCACTTATG
AACAGATATG
TAACTAATAA
GTTTGATACC
CAGCTCTTCC
ATACAATAGG
TGTGGAATTT
TTAAATAAAG
ATTTGGAAGT
GGATGGACAT
TTTGTTACCA
TGCAGATTTG
GGACACGGCA
GGTCAGGAGC
GATTCCGAAG
CCTGAGGACA
CCATTTTACA
GAGGTTCTGA
CTGCTGCCTG
CTTACTTTTA
GTGTCGATGA
TTCACAAAGC
TTCCAGAACT
TAAGTAACTG
GAAGAAAGAA
TTCATATATT
ATGCAGATGT
GAAAGAGCCT
GAGAGCTTTC
CTTTTGTGAT
TCTGGGTAAC
AAGATTGACA
TAAGCGAACG
GCAGGTGTCT
ACAGAAGAAG
CCCAAGCTTG
GTGCAGGGAC
AACGGCGACT
ATCCTTATTT
TGAAACAAGT
GCAAAAGATG
CCACAAATGT
GGCAGCAGCC
TTTGAGGAAG
CGGTTCGAAG
AGTTCTTGCT
ACCGAGGATA
GGTCAGATCA
TTTGATTCAG
ACAGACACAG
TCAATCTTCA
CCGAAAGCCC
AAGCCTAGCT
CATCTTGCTG
TTGAGCGGCC
GCGAAGGATC
TGCGATCGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACGGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGCTG
AAGCTTCGAG
GGGCTCGCAT
CTCTCCTTCA
CGCGCCCGCC
GCCCTACCTG
AGGCCGCCAT
CCACGCCGGT
TGAGTCGCGT
TCTGCCGCCT
CCCGCCTGTG
GTGCCTCCTG
AACTGCGTCC
GCCGTCTAGG
TAAGTTTAAA
GCTCAGGTCG
AGACCGGGCC
TTTGTCCGGC
GCTCCCTTGG
AGCCTACCTA
GACTCAGCCG
GCTCTCCACG
CTTTGCCTGA
CCCTGCTTGC
TCAACTCTAC
GTCTTTGTTT
CGTTTTCTGT
TCTGCGCCGT
TACAGATCCA
AGCTGTGACC
GGCGCCTACG
CTAGATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCCGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGACCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
CGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CTGAGTCGAC
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAATCAT
CGTCCTTTCC
TTGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGGTACCTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAA
ATAAGATCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTAG
TAGTTCATGT
CATCTTATTA
TTCAGTATTT
ATAACTTGCA
AAGAAATGAA
TATCAGAGAG
TGAGAGGAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGC
TCTAGCTATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCGGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGACTT
TTGCAGAGAC
GGCCCAAATT
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGGACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTCTA
AGAAACCATT
ATTATCATGA
CATTAACCTA
TAAAAATAGG
CGTATCACGA
GGCCCTTTCG
TCTCGCGCGT
TTCGGTGATG
ACGGTGAAAA
CCTCTGACAC
ATGCAGCTCC
CGGAGACGGT
CACAGCTTGT
CTGTAAGCGG
ATGCCGGGAG
CAGACAAGCC
CGTCAGGGCG
CGTCAGCGGG
TGTTGGCGGG
TGTCGGGGCT
GGCTTAACTA
TGCGGCATCA
GAGCAGATTG
TACTGAGAGT
GCACCATATG
CGGTGTGAAA
TACCGCACAG
ATGCGTAAGG
AGAAAATACC
GCATCAGGCG
CCATTCGCCA
TTCAGGCTGC
GCAACTGTTG
GGAAGGGCGA
TCGGTGCGGG
CCTCTTCGCT
ATTACGCCAG
CTGGCGAAAG
GGGGATGTGC
TGCAAGGCGA
TTAAGTTGGG
TAACGCCAGG
GTTTTCCCAG
TCACGACGTT
GTAAAACGAC
GGCCAGTGCC
AAGCTG