名称: | pLI50-pRPSL-LuxR-PLux-NLL-MetRS-mCherry |
---|---|
别称: | - |
基因长: | 2034bp |
质粒宿主: | 金黄色葡萄球菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 大肠杆菌 |
原核抗性: | Amp |
筛选标记: | Chl |
荧光标记: | 红色 |
启动子: | Rpsl |
复制子: | Ec |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pLI50-pRPSL-EGFP |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLI50-pRPSL-LuxR-PLux-NLL-MetRS-mCherry是适用于金黄色葡萄球菌的低拷贝表达质粒,质粒携带Rpsl启动子可启动基因转录,该质粒携带Chl抗性基因,利于筛选出宿主菌。质粒携带的mCherry红色荧光蛋白,便于直观地观察目的基因表达的位置和水平等
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTCTTCA
AGAATTCTCA
GAAAAATATA
CCTGTATCTT
TTTTCAAAAG
CAAACATGCT
TTGGGTAAAC
ATGTAGGTAT
TAACGTCAAT
GCGACAATAG
TAGCATTGAT
TAAATGAGAA
TTAGTAAGTG
TTTTACTTAC
TAAATTTTAT
TTAACCTAAA
AATGAACCAC
CTGGATGTGT
GGGATTAAAA
AGTGAAGAGA
GGAGGACATA
TCACTTAGGA
GGATGATTAT
TTGAGCTCTA
CTAGAGAAAG
AGGAGAAATA
CTAGATGAAA
AACATAAATG
CCGACGACAC
ATACAGAATA
ATTAATAAAA
TTAAAGCTTG
TAGAAGCAAT
AATGATATTA
ATCAATGCTT
ATCTGATATG
ACTAAAATGG
TACATTGTGA
ATATTATTTA
CTCGCGATCA
TTTATCCTCA
TTCTATGGTT
AAATCTGATA
TTTCAATCCT
AGATAATTAC
CCTAAAAAAT
GGAGGCAATA
TTATGATGAC
GCTAATTTAA
TAAAATATGA
TCCTATAGTA
GATTATTCTA
ACTCCAATCA
TTCACCAATT
AATTGGAATA
TATTTGAAAA
CAATGCTGTA
AATAAAAAAT
CTCCAAATGT
AATTAAAGAA
GCGAAAACAT
CAGGTCTTAT
CACTGGGTTT
AGTTTCCCTA
TTCATACGGC
TAACAATGGC
TTCGGAATGC
TTAGTTTTGC
ACATTCAGAA
AAAGACAACT
ATATAGATAG
TTTATTTTTA
CATGCGTGTA
TGAACATACC
ATTAATTGTT
CCTTCTCTAG
TTGATAATTA
TCGAAAAATA
AATATAGCAA
ATAATAAATC
AAACAACGAT
TTAACCAAAA
GAGAAAAAGA
ATGTTTAGCG
TGGGCATGCG
AAGGAAAAAG
CTCTTGGGAT
ATTTCAAAAA
TATTAGGTTG
CAGTGAGCGT
ACTGTCACTT
TCCATTTAAC
CAATGCGCAA
ATGAAACTCA
ATACAACAAA
CCGCTGCCAA
AGTATTTCTA
AAGCAATTTT
AACAGGAGCA
ATTGATTGCC
CATACTTTAA
AAATTAATAA
CACTGATAGT
GCTAGTGTAG
ATCACTACTA
GAGCCAGGCA
TCAAATAAAA
CGAAAGGCTC
AGTCGAAAGA
CTGGGCCTTT
CGTTTTATCT
GTTGTTTGTC
GGTGAACGCT
CTCTACTAGA
GTCACACTGG
CTCACCTTCG
GGTGGGCCTT
TCTGCGTTTA
TATACTAGAG
ACCTGTAGGA
TCGTACAGGT
TTACGCAAGA
AAATGGTTTG
TTATAGTCGA
ATAAATACTA
GAGTCACACA
GGAAAGTACT
AGATGACTCA
AGTCGCGAAG
AAAATTCTGG
TGACGTGCGC
AAACCCGTAC
GCTAACGGCT
CAATCCACCT
CGGCCATATG
CTGGAGCACA
TCCAGGCTGA
TGTCTGGGTC
CGTTACCAGC
GAATGCGCGG
CCACGAGGTC
AACTTCATCT
GCGCCGACGA
TGCCCACGGT
ACACCGATCA
TGCTGAAAGC
TCAGCAGCTT
GGTATCACCC
CGGAGCAGAT
GATTGGCGAA
ATGAGTCAGG
AGCATCAGAC
TGATTTCGCA
GGCTTTAACA
TCAGCTATGA
CAACTATCAC
TCGACGCACA
GCGAAGAGAA
CCGCCAGTTG
TCAGAACTTA
TCTACTCTCG
CCTGAAAGAA
AACGGTTTTA
TTAAAAACCG
CACCATCTCT
CAGCTGTACG
ATCCGGAAAA
AGGCATGTTC
CTGCCGGACC
GTTTTGTGAA
AGGCACCTGC
CCGAAATGTA
AATCCCCGGA
TCAATACGGC
GATAACTGCG
AAGTCTGCGG
CGCGACCTAC
AGCCCGACTG
AACTGATCGA
GCCGAAATCG
GTGGTTTCTG
GCGCTACGCC
GGTAATGCGT
GATTCTGAAC
ACTTCTTCTT
TGATCTGCCC
TCTTTCAGCG
AAATGTTGCA
GGCATGGACC
CGCAGCGGTG
CGTTGCAGGA
GCAGGTGGCA
AATAAAATGC
AGGAGTGGTT
TGAATCTGGC
CTGCAACAGT
GGGATATCTC
CCGCGACGCC
CCTTACTTCG
GTTTTGAAAT
TCCGAACGCG
CCGGGCAAAT
ATTTCTACGT
CTGGCTGGAC
GCACCGATTG
GCCTGATGGG
TTCTTTCAAG
AATCTGTGCG
ACAAGCGCGG
CGACAGCGTA
AGCTTCGATG
AATACTGGAA
GAAAGACTCC
ACCGCCGAGC
TGTACCACTT
CATCGGTAAA
GATATTGTTT
ACTTCCTGAG
CCTGTTCTGG
CCTGCCATGC
TGGAAGGCAG
CAACTTCCGC
AAGCCGTCCA
ACCTGTTTGT
TCATGGCTAT
GTGACGGTGA
ACGGCGCAAA
GATGTCCAAG
TCTCGCGGCA
CCTTTATTAA
AGCCAGCACC
TGGCTGAATC
ATTTTGACGC
AGACAGCCTG
CGTTACTACT
ACACTGCGAA
ACTCTCTTCG
CGCATTGATG
ATATCGATCT
CAACCTGGAA
GATTTCGTTC
AGCGTGTGAA
TGCCGATATC
GTTAACAAAG
TGGTTAACCT
GGCCTCCCGT
AATGCGGGCT
TTATCAACAA
GCGTTTTGAC
GGCGTGCTGG
CAAGCGAACT
GGCTGACCCG
CAGTTGTACA
AAACCTTCAC
TGATGCCGCT
GAAGTGATTG
GTGAAGCGTG
GGAAAGCCGT
GAATTTGGTA
AAGCCGTGCG
CGAAATCATG
GCGCTGGCTG
ATCTGGCTAA
CCGCTATGTC
GATGAACAGG
CTCCGTGGGT
GGTGGCGAAA
CAGGAAGGCC
GCGATGCCGA
CCTGCAGGCA
ATTTGCTCAA
TGGGCATCAA
CCTGTTCCGC
GTGCTGATGA
CTTACCTGAA
GCCGGTACTG
CCGAAACTGA
CCGAGCGTGC
AGAAGCATTC
CTCAATACGG
AACTGACCTG
GGATGGTATC
CAGCAACCGC
TGCTGGGCCA
CAAAGTGAAT
CCGTTCAAGG
CGCTGTATAA
CCGCATCGAT
ATGAGGCAGG
TTGAAGCACT
GGTGGAAGCC
TCTAAATGAG
AAGTAAAAGC
CGCTGCCGCG
CCGGTAACTG
GCCCGCTGGC
AGATGATCCG
ATTCAGGAAA
CCATCACCTT
TGACGACTTC
GCTAAAGTTG
ACCTGCGCGT
GGCGCTGATT
GAAAACGCAG
AGTTTGTTGA
AGGTTCTGAC
AAACTGCTGC
GCCTGACGCT
GGATCTCGGC
GGTGAAAAAC
GCAATGTCTT
CTCCGGTATT
CGTTCTGCTT
ACCCGGATCC
GCAGGCACTG
ATTGGTCGTC
ACACCATTAT
GGTGGCTAAC
CTGGCACCAC
GTAAAATGCG
CTTCGGTATC
TCTGAAGGCA
TGGTGATGGC
TGCCGGTCCT
GGCGGGAAAG
ATATTTTCCT
GCTAAGCCCG
GATGCCGGTG
CTAAACCGGG
TCATCAGGTG
AAATAAGGAT
CCGGTGGAGG
AGGCTCTGGT
GGAGGCGGTA
GCGGAGGCGG
AGGGTCGTCT
AGAATGGTGA
GCAAGGGCGA
GGAGGATAAC
ATGGCCATCA
TCAAGGAGTT
CATGCGCTTC
AAGGTGCACA
TGGAGGGCTC
CGTGAACGGC
CACGAGTTCG
AGATCGAGGG
CGAGGGCGAG
GGCCGCCCCT
ACGAGGGCAC
CCAGACCGCC
AAGCTGAAGG
TGACCAAGGG
TGGCCCCCTG
CCCTTCGCCT
GGGACATCCT
GTCCCCTCAG
TTCATGTACG
GCTCCAAGGC
CTACGTGAAG
CACCCCGCCG
ACATCCCCGA
CTACTTGAAG
CTGTCCTTCC
CCGAGGGCTT
CAAGTGGGAG
CGCGTGATGA
ACTTCGAGGA
CGGCGGCGTG
GTGACCGTGA
CCCAGGACTC
CTCCCTGCAG
GACGGCGAGT
TCATCTACAA
GGTGAAGCTG
CGCGGCACCA
ACTTCCCCTC
CGACGGCCCC
GTAATGCAGA
AGAAGACCAT
GGGCTGGGAG
GCCTCCTCCG
AGCGGATGTA
CCCCGAGGAC
GGCGCCCTGA
AGGGCGAGAT
CAAGCAGAGG
CTGAAGCTGA
AGGACGGCGG
CCACTACGAC
GCTGAGGTCA
AGACCACCTA
CAAGGCCAAG
AAGCCCGTGC
AGCTGCCCGG
CGCCTACAAC
GTCAACATCA
AGTTGGACAT
CACCTCCCAC
AACGAGGACT
ACACCATCGT
GGAACAGTAC
GAACGCGCCG
AGGGCCGCCA
CTCCACCGGC
GGCATGGACG
AGCTGTACAA
GTAAGTCGAC
CTGCAGGCAT
GCAAGCTTCT
GTAGGTTTTT
AGGCATAAAA
CTATATGATT
TACCCCTAAA
TCTTTAAAAT
GCCCCTTAAA
ATTCAAAATA
AAGGCATTTA
AAATTTAAAT
ATTTCTTGTG
ATAAAGTTTG
TTAAAAAGGA
GTGGTTTTAT
GACTGTTATG
TGGTTATCGA
TTATAGGTAT
GTGGTTTTGT
ATTGGAATGG
CATTTTTTGC
TATCAAGGTT
ATTAAAAATA
AAAATTAGAC
CACGCATTTA
TGCCGAGAAA
ATTTATTGTG
CGTTGAGAAG
AACCCTTAAC
TAAACTTGCA
GACGAATGTC
GGCATAGCGT
GAGCTATTAA
GCCGACCATT
CGACAAGTTT
TGGGATTGTT
AAGGGTTCCG
AGGCTCAACG
TCAATAAAGC
AATTGGAATA
AAGAAGCGAA
AAAGGAGAAG
TCGGTTCAGA
AAAAGAAGGA
TATGGATCTG
GAGCTGTAAT
ATAAAAACCT
TCTTCAACTA
ACGGGGCAGG
TTAGTGACAT
TAGAAAACCG
ACTGTAAAAA
GTACAGTCGG
CATTATCTCA
TATTATAAAA
GCCAGTCATT
AGGCCTATCT
GACAATTCCT
GAATAGAGTT
CATAAACAAT
CCTGCATGAT
AACCATCACA
AACAGAATGA
TGTACCTGTA
AAGATAGCGG
TAAATATATT
GAATTACCTT
TATTAATGAA
TTTTCCTGCT
GTAATAATGG
GTAGAAGGTA
ATTACTATTA
TTATTGATAT
TTAAGTTAAA
CCCAGTAAAT
GAAGTCCATG
GAATAATAGA
AAGAGAAAAA
GCATTTTCAG
GTATAGGTGT
TTTGGGAAAC
AATTTCCCCG
AACCATTATA
TTTCTCTACA
TCAGAAAGGT
ATAAATCATA
AAACTCTTTG
AAGTCATTCT
TTACAGGAGT
CCAAATACCA
GAGAATGTTT
TAGATACACC
ATCAAAAATT
GTATAAAGTG
GCTCTAACTT
ATCCCAATAA
CCTAACTCTC
CGTCGCTATT
GTAACCAGTT
CTAAAAGCTG
TATTTGAGTT
TATCACCCTT
GTCACTAAGA
AAATAAATGC
AGGGTAAAAT
TTATATCCTT
CTTGTTTTAT
GTTTCGGTAT
AAAACACTAA
TATCAATTTC
TGTGGTTATA
CTAAAAGTCG
TTTGTTGGTT
CAAATAATGA
TTAAATATCT
CTTTTCTCTT
CCAATTGTCT
AAATCAATTT
TATTAAAGTT
CATTTGATAT
GCCTCCTAAA
TTTTTATCTA
AAGTGAATTT
AGGAGGCTTA
CTTGTCTGCT
TTCTTCATTA
GAATCAATCC
TTTTTTTAAA
AGTCAATATT
ACTGTAACAT
AAATATATAT
TTTAAAAATA
TCCCACTTTA
TCCAATTTTC
GTTTGTTGAA
TTTTTTTAAA
AGTCAATATT
ACTGTAACAT
AAATATATAT
TTTAAAAATA
TCCCACTTTA
TCCAATTTTC
GTTTGTTGAA
CTAATGGGTG
CTTTAGTTGA
AGAATAAAAG
ACCACATTAA
AAAATGTGGT
CTTTTGTGTT
TTTTTAAAGG
ATTTGAGCGT
AGCGAAAAAT
CCTTTTCTTT
CTTATCTTGA
TACATATAGA
AATAACGTCA
TTTTTATTTT
AGTTGCTGAA
AGGTGCGTTG
AAGTGTTGGT
ATGTATGTGT
TTTAAAGTAT
TGAAAACCCT
TAAAATTGGT
TGCACAGAAA
AACCCCATCT
GTTAAAGTTA
TAAGTGACTA
AACAAATAAC
TAAATAGATG
GGGGTTTCTT
TTAATATTAT
GTGTCCTAAT
AGTAGCATTT
ATTCAGATGA
AAAATCAAGG
GTTTTAGTGG
ACAAGACAAA
AAGTGGAAAA
GTGAGACCAT
GGAGAGAAAA
GAAAATCGCT
AATGTTGATT
ACTTTGAACT
TCTGCATATT
CTTGAATTTA
AAAAGGCTGA
AAGAGTAAAA
GATTGTGCTG
AAATATTAGA
GTATAAACAA
AATCGTGAAA
CAGGCGAAAG
AAAGTTGTAT
CGAGTGTGGT
TTTGTAAATC
CAGGCTTTGT
CCAATGTGCA
ACTGGAGGAG
AGCAATGAAA
CATGGCATTC
AGTCACAAAA
GGTTGTTGCT
GAAGTTATTA
AACAAAAGCC
AACAGTTCGT
TGGTTGTTTC
TCACATTAAC
AGTTAAAAAT
GTTTATGATG
GCGAAGAATT
AAATAAGAGT
TTGTCAGATA
TGGCTCAAGG
ATTTCGCCGA
ATGATGCAAT
ATAAAAAAAT
TAATAAAAAT
CTTGTTGGTT
TTATGCGTGC
AACGGAAGTG
ACAATAAATA
ATAAAGATAA
TTCTTATAAT
CAGCACATGC
ATGTATTGGT
ATGTGTGGAA
CCAACTTATT
TTAAGAATAC
AGAAAACTAC
GTGAATCAAA
AACAATGGAT
TCAATTTTGG
AAAAAGGCAA
TGAAATTAGA
CTATGATCCA
AATGTAAAAG
TTCAAATGAT
TCGACCGAAA
AATAAATATA
AATCGGATAT
ACAATCGGCA
ATTGACGAAA
CTGCAAAATA
TCCTGTAAAG
GATACGGATT
TTATGACCGA
TGATGAAGAA
AAGAATTTGA
AACGTTTGTC
TGATTTGGAG
GAAGGTTTAC
ACCGTAAAAG
GTTAATCTCC
TATGGTGGTT
TGTTAAAAGA
AATACATAAA
AAATTAAACC
TTGATGACAC
AGAAGAAGGC
GATTTGATTC
ATACAGATGA
TGACGAAAAA
GCCGATGAAG
ATGGATTTTC
TATTATTGCA
ATGTGGAATT
GGGAACGGAA
AAATTATTTT
ATTAAAGAGT
AGTTCAACAA
ACGGGCCAGT
TTGTTGAAGA
TTAGATGCTA
TAATTGTTAT
TAAAAGGATT
GAAGGATGCT
TAGGAAGACG
AGTTATTAAT
AGCTGAATAA
GAACGGTGCT
CTCCAAATAT
TCTTATTTAG
AAAAGCAAAT
CTAAAATTAT
CTGAAAAGGG
AATGAGAATA
GTGAATGGAC
CAATAATAAT
GACTAGAGAA
GAAAGAATGA
AGATTGTTCA
TGAAATTAAG
GAACGAATAT
TGGATAAATA
TGGGGATGAT
GTTAAGGCTA
TTGGTGTTTA
TGGCTCTCTT
GGTCGTCAGA
CTGATGGGCC
CTATTCGGAT
ATTGAGATGA
TGTGTGTCAT
GTCAACAGAG
GAAGCAGAGT
TCAGCCATGA
ATGGACAACC
GGAAAGAGCG
AAAATGCCTC
ACATTTGTGC
CACCTAAAAA
GGAGCGATTT
ACATATGAGT
TATGCAGTTT
GTAGAATGCA
AAAAGTGAAA
TCAGCTGCCT
CGCGCGTTTC
GGTGATGACG
GTGAAAACCT
CTGACACATG
CAGCTCCCGG
AGACGGTCAC
AGCTTGTCTG
TAAGCGGATG
CCGGGAGCAG
ACAAGCCCGT
CAGGGCGCGT
CAGCGGGTGT
TGGCGGGTGT
CGGGGCGCAG
CCATGACCCA
GTCACGTAGC
GATAGCGGAG
TGTATACTGG
CTTAACTATG
CGGCATCAGA
GCAGATTGTA
CTGAGAGTGC
ACCATATGCG
GTGTGAAATA
CCGCACAGAT
GCGTAAGGAG
AAAATACCGC
ATCAGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGGACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTGCAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAACAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCTAA
GAAACCATTA
TTATC