产品货号：P72451
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pX333-U6-Atg16l1(rat)-U6-Atg16l2(rat)-sgRNA3-CBH-Cas9-Puro |
|---|---|
| 别称: | acagaccatctctgacctgg；acagcgggacagtgtcagag |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 大鼠 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | |
| 启动子: | U6 |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pX333-2×(U6-gRNA)-CBH-Cas9-Puro |
| 正向引物: | U6-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pX333-U6-Atg16l1(rat)-U6-Atg16l2(rat)-sgRNA3-CBH-Cas9-Puro是适用于哺乳细胞基因编辑的质粒。质粒带有的U6启动子可启动针对大鼠源Atg16l1基因设计的sgRNA片段的转录，促使Cas9能在sgRNA的引导下精准地识别并切割目标基因的双链 DNA。质粒带有Puro抗性基因，可筛选阳性宿主细胞。
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GGCCTTTTGC
TCACATGCGC
CATTCTCGAG
CATGCGCCAT
TCTCGAGCAT
GCTCGAGAAT
GGCGCATGTG
AGGGCCTATT
TCCCATGATT
CCTTCATATT
TGCATATACG
ATACAAGGCT
GTTAGAGAGA
TAATTGGAAT
TAATTTGACT
GTAAACACAA
AGATATTAGT
ACAAAATACG
TGACGTAGAA
AGTAATAATT
TCTTGGGTAG
TTTGCAGTTT
TAAAATTATG
TTTTAAAATG
GACTATCATA
TGCTTACCGT
AACTTGAAAG
TATTTCGATT
TCTTGGCTTT
ATATATCTTG
TGGAAAGGAC
GAAACACCGA
CAGACCATCT
CTGACCTGGG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTTTTAG
CGCGTGCGCC
AATTCTGCAG
ACAAATGGCT
CTAGAGAGGG
CCTATTTCCC
ATGATTCCTT
CATATTTGCA
TATACGATAC
AAGGCTGTTA
GAGAGATAAT
TGGAATTAAT
TTGACTGTAA
ACACAAAGAT
ATTAGTACAA
AATACGTGAC
GTAGAAAGTA
ATAATTTCTT
GGGTAGTTTG
CAGTTTTAAA
ATTATGTTTT
AAAATGGACT
ATCATATGCT
TACCGTAACT
TGAAAGTATT
TCGATTTCTT
GGCTTTATAT
ATCTTGTGGA
AAGGACGAAA
CACCGACAGC
GGGACAGTGT
CAGAGGTTTT
AGAGCTAGAA
ATAGCAAGTT
AAAATAAGGC
TAGTCCGTTA
TCAACTTGAA
AAAGTGGCAC
CGAGTCGGTG
CTTTTTTGTT
TTAGAGCTAG
AAATAGCAAG
TTAAAATAAG
GCTAGTCCGT
TTTTAGCGCG
TGCGCCAATT
CTGCAGACAA
ATGGGGTACC
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
GTAACGCCAA
TAGGGACTTT
CCATTGACGT
CAATGGGTGG
AGTATTTACG
GTAAACTGCC
CACTTGGCAG
TACATCAAGT
GTATCATATG
CCAAGTACGC
CCCCTATTGA
CGTCAATGAC
GGTAAATGGC
CCGCCTGGCA
TTGTGCCCAG
TACATGACCT
TATGGGACTT
TCCTACTTGG
CAGTACATCT
ACGTATTAGT
CATCGCTATT
ACCATGGTCG
AGGTGAGCCC
CACGTTCTGC
TTCACTCTCC
CCATCTCCCC
CCCCTCCCCA
CCCCCAATTT
TGTATTTATT
TATTTTTTAA
TTATTTTGTG
CAGCGATGGG
GGCGGGGGGG
GGGGGGGGGC
GCGCGCCAGG
CGGGGCGGGG
CGGGGCGAGG
GGCGGGGCGG
GGCGAGGCGG
AGAGGTGCGG
CGGCAGCCAA
TCAGAGCGGC
GCGCTCCGAA
AGTTTCCTTT
TATGGCGAGG
CGGCGGCGGC
GGCGGCCCTA
TAAAAAGCGA
AGCGCGCGGC
GGGCGGGAGT
CGCTGCGCGC
TGCCTTCGCC
CCGTGCCCCG
CTCCGCCGCC
GCCTCGCGCC
GCCCGCCCCG
GCTCTGACTG
ACCGCGTTAC
TCCCACAGGT
GAGCGGGCGG
GACGGCCCTT
CTCCTCCGGG
CTGTAATTAG
CTGAGCAAGA
GGTAAGGGTT
TAAGGGATGG
TTGGTTGGTG
GGGTATTAAT
GTTTAATTAC
CTGGAGCACC
TGCCTGAAAT
CACTTTTTTT
CAGGTTGGAC
CGGTGCCACC
ATGGACTATA
AGGACCACGA
CGGAGACTAC
AAGGATCATG
ATATTGATTA
CAAAGACGAT
GACGATAAGA
TGGCCCCAAA
GAAGAAGCGG
AAGGTCGGTA
TCCACGGAGT
CCCAGCAGCC
GACAAGAAGT
ACAGCATCGG
CCTGGACATC
GGCACCAACT
CTGTGGGCTG
GGCCGTGATC
ACCGACGAGT
ACAAGGTGCC
CAGCAAGAAA
TTCAAGGTGC
TGGGCAACAC
CGACCGGCAC
AGCATCAAGA
AGAACCTGAT
CGGAGCCCTG
CTGTTCGACA
GCGGCGAAAC
AGCCGAGGCC
ACCCGGCTGA
AGAGAACCGC
CAGAAGAAGA
TACACCAGAC
GGAAGAACCG
GATCTGCTAT
CTGCAAGAGA
TCTTCAGCAA
CGAGATGGCC
AAGGTGGACG
ACAGCTTCTT
CCACAGACTG
GAAGAGTCCT
TCCTGGTGGA
AGAGGATAAG
AAGCACGAGC
GGCACCCCAT
CTTCGGCAAC
ATCGTGGACG
AGGTGGCCTA
CCACGAGAAG
TACCCCACCA
TCTACCACCT
GAGAAAGAAA
CTGGTGGACA
GCACCGACAA
GGCCGACCTG
CGGCTGATCT
ATCTGGCCCT
GGCCCACATG
ATCAAGTTCC
GGGGCCACTT
CCTGATCGAG
GGCGACCTGA
ACCCCGACAA
CAGCGACGTG
GACAAGCTGT
TCATCCAGCT
GGTGCAGACC
TACAACCAGC
TGTTCGAGGA
AAACCCCATC
AACGCCAGCG
GCGTGGACGC
CAAGGCCATC
CTGTCTGCCA
GACTGAGCAA
GAGCAGACGG
CTGGAAAATC
TGATCGCCCA
GCTGCCCGGC
GAGAAGAAGA
ATGGCCTGTT
CGGAAACCTG
ATTGCCCTGA
GCCTGGGCCT
GACCCCCAAC
TTCAAGAGCA
ACTTCGACCT
GGCCGAGGAT
GCCAAACTGC
AGCTGAGCAA
GGACACCTAC
GACGACGACC
TGGACAACCT
GCTGGCCCAG
ATCGGCGACC
AGTACGCCGA
CCTGTTTCTG
GCCGCCAAGA
ACCTGTCCGA
CGCCATCCTG
CTGAGCGACA
TCCTGAGAGT
GAACACCGAG
ATCACCAAGG
CCCCCCTGAG
CGCCTCTATG
ATCAAGAGAT
ACGACGAGCA
CCACCAGGAC
CTGACCCTGC
TGAAAGCTCT
CGTGCGGCAG
CAGCTGCCTG
AGAAGTACAA
AGAGATTTTC
TTCGACCAGA
GCAAGAACGG
CTACGCCGGC
TACATTGACG
GCGGAGCCAG
CCAGGAAGAG
TTCTACAAGT
TCATCAAGCC
CATCCTGGAA
AAGATGGACG
GCACCGAGGA
ACTGCTCGTG
AAGCTGAACA
GAGAGGACCT
GCTGCGGAAG
CAGCGGACCT
TCGACAACGG
CAGCATCCCC
CACCAGATCC
ACCTGGGAGA
GCTGCACGCC
ATTCTGCGGC
GGCAGGAAGA
TTTTTACCCA
TTCCTGAAGG
ACAACCGGGA
AAAGATCGAG
AAGATCCTGA
CCTTCCGCAT
CCCCTACTAC
GTGGGCCCTC
TGGCCAGGGG
AAACAGCAGA
TTCGCCTGGA
TGACCAGAAA
GAGCGAGGAA
ACCATCACCC
CCTGGAACTT
CGAGGAAGTG
GTGGACAAGG
GCGCTTCCGC
CCAGAGCTTC
ATCGAGCGGA
TGACCAACTT
CGATAAGAAC
CTGCCCAACG
AGAAGGTGCT
GCCCAAGCAC
AGCCTGCTGT
ACGAGTACTT
CACCGTGTAT
AACGAGCTGA
CCAAAGTGAA
ATACGTGACC
GAGGGAATGA
GAAAGCCCGC
CTTCCTGAGC
GGCGAGCAGA
AAAAGGCCAT
CGTGGACCTG
CTGTTCAAGA
CCAACCGGAA
AGTGACCGTG
AAGCAGCTGA
AAGAGGACTA
CTTCAAGAAA
ATCGAGTGCT
TCGACTCCGT
GGAAATCTCC
GGCGTGGAAG
ATCGGTTCAA
CGCCTCCCTG
GGCACATACC
ACGATCTGCT
GAAAATTATC
AAGGACAAGG
ACTTCCTGGA
CAATGAGGAA
AACGAGGACA
TTCTGGAAGA
TATCGTGCTG
ACCCTGACAC
TGTTTGAGGA
CAGAGAGATG
ATCGAGGAAC
GGCTGAAAAC
CTATGCCCAC
CTGTTCGACG
ACAAAGTGAT
GAAGCAGCTG
AAGCGGCGGA
GATACACCGG
CTGGGGCAGG
CTGAGCCGGA
AGCTGATCAA
CGGCATCCGG
GACAAGCAGT
CCGGCAAGAC
AATCCTGGAT
TTCCTGAAGT
CCGACGGCTT
CGCCAACAGA
AACTTCATGC
AGCTGATCCA
CGACGACAGC
CTGACCTTTA
AAGAGGACAT
CCAGAAAGCC
CAGGTGTCCG
GCCAGGGCGA
TAGCCTGCAC
GAGCACATTG
CCAATCTGGC
CGGCAGCCCC
GCCATTAAGA
AGGGCATCCT
GCAGACAGTG
AAGGTGGTGG
ACGAGCTCGT
GAAAGTGATG
GGCCGGCACA
AGCCCGAGAA
CATCGTGATC
GAAATGGCCA
GAGAGAACCA
GACCACCCAG
AAGGGACAGA
AGAACAGCCG
CGAGAGAATG
AAGCGGATCG
AAGAGGGCAT
CAAAGAGCTG
GGCAGCCAGA
TCCTGAAAGA
ACACCCCGTG
GAAAACACCC
AGCTGCAGAA
CGAGAAGCTG
TACCTGTACT
ACCTGCAGAA
TGGGCGGGAT
ATGTACGTGG
ACCAGGAACT
GGACATCAAC
CGGCTGTCCG
ACTACGATGT
GGACCATATC
GTGCCTCAGA
GCTTTCTGAA
GGACGACTCC
ATCGACAACA
AGGTGCTGAC
CAGAAGCGAC
AAGAACCGGG
GCAAGAGCGA
CAACGTGCCC
TCCGAAGAGG
TCGTGAAGAA
GATGAAGAAC
TACTGGCGGC
AGCTGCTGAA
CGCCAAGCTG
ATTACCCAGA
GAAAGTTCGA
CAATCTGACC
AAGGCCGAGA
GAGGCGGCCT
GAGCGAACTG
GATAAGGCCG
GCTTCATCAA
GAGACAGCTG
GTGGAAACCC
GGCAGATCAC
AAAGCACGTG
GCACAGATCC
TGGACTCCCG
GATGAACACT
AAGTACGACG
AGAATGACAA
GCTGATCCGG
GAAGTGAAAG
TGATCACCCT
GAAGTCCAAG
CTGGTGTCCG
ATTTCCGGAA
GGATTTCCAG
TTTTACAAAG
TGCGCGAGAT
CAACAACTAC
CACCACGCCC
ACGACGCCTA
CCTGAACGCC
GTCGTGGGAA
CCGCCCTGAT
CAAAAAGTAC
CCTAAGCTGG
AAAGCGAGTT
CGTGTACGGC
GACTACAAGG
TGTACGACGT
GCGGAAGATG
ATCGCCAAGA
GCGAGCAGGA
AATCGGCAAG
GCTACCGCCA
AGTACTTCTT
CTACAGCAAC
ATCATGAACT
TTTTCAAGAC
CGAGATTACC
CTGGCCAACG
GCGAGATCCG
GAAGCGGCCT
CTGATCGAGA
CAAACGGCGA
AACCGGGGAG
ATCGTGTGGG
ATAAGGGCCG
GGATTTTGCC
ACCGTGCGGA
AAGTGCTGAG
CATGCCCCAA
GTGAATATCG
TGAAAAAGAC
CGAGGTGCAG
ACAGGCGGCT
TCAGCAAAGA
GTCTATCCTG
CCCAAGAGGA
ACAGCGATAA
GCTGATCGCC
AGAAAGAAGG
ACTGGGACCC
TAAGAAGTAC
GGCGGCTTCG
ACAGCCCCAC
CGTGGCCTAT
TCTGTGCTGG
TGGTGGCCAA
AGTGGAAAAG
GGCAAGTCCA
AGAAACTGAA
GAGTGTGAAA
GAGCTGCTGG
GGATCACCAT
CATGGAAAGA
AGCAGCTTCG
AGAAGAATCC
CATCGACTTT
CTGGAAGCCA
AGGGCTACAA
AGAAGTGAAA
AAGGACCTGA
TCATCAAGCT
GCCTAAGTAC
TCCCTGTTCG
AGCTGGAAAA
CGGCCGGAAG
AGAATGCTGG
CCTCTGCCGG
CGAACTGCAG
AAGGGAAACG
AACTGGCCCT
GCCCTCCAAA
TATGTGAACT
TCCTGTACCT
GGCCAGCCAC
TATGAGAAGC
TGAAGGGCTC
CCCCGAGGAT
AATGAGCAGA
AACAGCTGTT
TGTGGAACAG
CACAAGCACT
ACCTGGACGA
GATCATCGAG
CAGATCAGCG
AGTTCTCCAA
GAGAGTGATC
CTGGCCGACG
CTAATCTGGA
CAAAGTGCTG
TCCGCCTACA
ACAAGCACCG
GGATAAGCCC
ATCAGAGAGC
AGGCCGAGAA
TATCATCCAC
CTGTTTACCC
TGACCAATCT
GGGAGCCCCT
GCCGCCTTCA
AGTACTTTGA
CACCACCATC
GACCGGAAGA
GGTACACCAG
CACCAAAGAG
GTGCTGGACG
CCACCCTGAT
CCACCAGAGC
ATCACCGGCC
TGTACGAGAC
ACGGATCGAC
CTGTCTCAGC
TGGGAGGCGA
CAAAAGGCCG
GCGGCCACGA
AAAAGGCCGG
CCAGGCAAAA
AAGAAAAAGG
GATCTCGCGA
GGGCAGAGGA
AGCCTTCTAA
CATGCGGTGA
CGTGGAGGAG
AATCCCGGCC
CTATGACCGA
GTACAAGCCC
ACGGTGCGCC
TCGCCACCCG
CGACGACGTC
CCCAGGGCCG
TACGCACCCT
CGCCGCCGCG
TTCGCCGACT
ACCCCGCCAC
GCGCCACACC
GTCGATCCGG
ACCGCCACAT
CGAGCGGGTC
ACCGAGCTGC
AAGAACTCTT
CCTCACGCGC
GTCGGGCTCG
ACATCGGCAA
GGTGTGGGTC
GCGGACGACG
GCGCCGCGGT
GGCGGTCTGG
ACCACGCCGG
AGAGCGTCGA
AGCGGGGGCG
GTGTTCGCCG
AGATCGGCCC
GCGCATGGCC
GAGTTGAGCG
GTTCCCGGCT
GGCCGCGCAG
CAACAGATGG
AAGGCCTCCT
GGCGCCGCAC
CGGCCCAAGG
AGCCCGCGTG
GTTCCTGGCC
ACCGTCGGCG
TCTCGCCCGA
CCACCAGGGC
AAGGGTCTGG
GCAGCGCCGT
CGTGCTCCCC
GGAGTGGAGG
CGGCCGAGCG
CGCCGGGGTG
CCCGCCTTCC
TGGAGACATC
CGCGCCCCGC
AACCTCCCCT
TCTACGAGCG
GCTCGGCTTC
ACCGTCACCG
CCGACGTCGA
GGTGCCCGAA
GGACCGCGCA
CCTGGTGCAT
GACCCGCAAG
CCCGGTGCCT
GAGAATTCCT
AGAGCTCGCT
GATCAGCCTC
GACTGTGCCT
TCTAGTTGCC
AGCCATCTGT
TGTTTGCCCC
TCCCCCGTGC
CTTCCTTGAC
CCTGGAAGGT
GCCACTCCCA
CTGTCCTTTC
CTAATAAAAT
GAGGAAATTG
CATCGCATTG
TCTGAGTAGG
TGTCATTCTA
TTCTGGGGGG
TGGGGTGGGG
CAGGACAGCA
AGGGGGAGGA
TTGGGAAGAG
AATAGCAGGC
ATGCTGGGGA
GCGGCCGCAG
GAACCCCTAG
TGATGGAGTT
GGCCACTCCC
TCTCTGCGCG
CTCGCTCGCT
CACTGAGGCC
GGGCGACCAA
AGGTCGCCCG
ACGCCCGGGC
TTTGCCCGGG
CGGCCTCAGT
GAGCGAGCGA
GCGCGCAGCT
GCCTGCAGGG
GCGCCTGATG
CGGTATTTTC
TCCTTACGCA
TCTGTGCGGT
ATTTCACACC
GCATACGTCA
AAGCAACCAT
AGTACGCGCC
CTGTAGCGGC
GCATTAAGCG
CGGCGGGTGT
GGTGGTTACG
CGCAGCGTGA
CCGCTACACT
TGCCAGCGCC
TTAGCGCCCG
CTCCTTTCGC
TTTCTTCCCT
TCCTTTCTCG
CCACGTTCGC
CGGCTTTCCC
CGTCAAGCTC
TAAATCGGGG
GCTCCCTTTA
GGGTTCCGAT
TTAGTGCTTT
ACGGCACCTC
GACCCCAAAA
AACTTGATTT
GGGTGATGGT
TCACGTAGTG
GGCCATCGCC
CTGATAGACG
GTTTTTCGCC
CTTTGACGTT
GGAGTCCACG
TTCTTTAATA
GTGGACTCTT
GTTCCAAACT
GGAACAACAC
TCAACTCTAT
CTCGGGCTAT
TCTTTTGATT
TATAAGGGAT
TTTGCCGATT
TCGGTCTATT
GGTTAAAAAA
TGAGCTGATT
TAACAAAAAT
TTAACGCGAA
TTTTAACAAA
ATATTAACGT
TTACAATTTT
ATGGTGCACT
CTCAGTACAA
TCTGCTCTGA
TGCCGCATAG
TTAAGCCAGC
CCCGACACCC
GCCAACACCC
GCTGACGCGC
CCTGACGGGC
TTGTCTGCTC
CCGGCATCCG
CTTACAGACA
AGCTGTGACC
GTCTCCGGGA
GCTGCATGTG
TCAGAGGTTT
TCACCGTCAT
CACCGAAACG
CGCGAGACGA
AAGGGCCTCG
TGATACGCCT
ATTTTTATAG
GTTAATGTCA
TGATAATAAT
GGTTTCTTAG
ACGTCAGGTG
GCACTTTTCG
GGGAAATGTG
CGCGGAACCC
CTATTTGTTT
ATTTTTCTAA
ATACATTCAA
ATATGTATCC
GCTCATGAGA
CAATAACCCT
GATAAATGCT
TCAATAATAT
TGAAAAAGGA
AGAGTATGAG
TATTCAACAT
TTCCGTGTCG
CCCTTATTCC
CTTTTTTGCG
GCATTTTGCC
TTCCTGTTTT
TGCTCACCCA
GAAACGCTGG
TGAAAGTAAA
AGATGCTGAA
GATCAGTTGG
GTGCACGAGT
GGGTTACATC
GAACTGGATC
TCAACAGCGG
TAAGATCCTT
GAGAGTTTTC
GCCCCGAAGA
ACGTTTTCCA
ATGATGAGCA
CTTTTAAAGT
TCTGCTATGT
GGCGCGGTAT
TATCCCGTAT
TGACGCCGGG
CAAGAGCAAC
TCGGTCGCCG
CATACACTAT
TCTCAGAATG
ACTTGGTTGA
GTACTCACCA
GTCACAGAAA
AGCATCTTAC
GGATGGCATG
ACAGTAAGAG
AATTATGCAG
TGCTGCCATA
ACCATGAGTG
ATAACACTGC
GGCCAACTTA
CTTCTGACAA
CGATCGGAGG
ACCGAAGGAG
CTAACCGCTT
TTTTGCACAA
CATGGGGGAT
CATGTAACTC
GCCTTGATCG
TTGGGAACCG
GAGCTGAATG
AAGCCATACC
AAACGACGAG
CGTGACACCA
CGATGCCTGT
AGCAATGGCA
ACAACGTTGC
GCAAACTATT
AACTGGCGAA
CTACTTACTC
TAGCTTCCCG
GCAACAATTA
ATAGACTGGA
TGGAGGCGGA
TAAAGTTGCA
GGACCACTTC
TGCGCTCGGC
CCTTCCGGCT
GGCTGGTTTA
TTGCTGATAA
ATCTGGAGCC
GGTGAGCGTG
GAAGCCGCGG
TATCATTGCA
GCACTGGGGC
CAGATGGTAA
GCCCTCCCGT
ATCGTAGTTA
TCTACACGAC
GGGGAGTCAG
GCAACTATGG
ATGAACGAAA
TAGACAGATC
GCTGAGATAG
GTGCCTCACT
GATTAAGCAT
TGGTAACTGT
CAGACCAAGT
TTACTCATAT
ATACTTTAGA
TTGATTTAAA
ACTTCATTTT
TAATTTAAAA
GGATCTAGGT
GAAGATCCTT
TTTGATAATC
TCATGACCAA
AATCCCTTAA
CGTGAGTTTT
CGTTCCACTG
AGCGTCAGAC
CCCGTAGAAA
AGATCAAAGG
ATCTTCTTGA
GATCCTTTTT
TTCTGCGCGT
AATCTGCTGC
TTGCAAACAA
AAAAACCACC
GCTACCAGCG
GTGGTTTGTT
TGCCGGATCA
AGAGCTACCA
ACTCTTTTTC
CGAAGGTAAC
TGGCTTCAGC
AGAGCGCAGA
TACCAAATAC
TGTTCTTCTA
GTGTAGCCGT
AGTTAGGCCA
CCACTTCAAG
AACTCTGTAG
CACCGCCTAC
ATACCTCGCT
CTGCTAATCC
TGTTACCAGT
GGCTGCTGCC
AGTGGCGATA
AGTCGTGTCT
TACCGGGTTG
GACTCAAGAC
GATAGTTACC
GGATAAGGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCTA
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCCTGGC
CTTTTGCT
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