名称: | pLV3-CMV-Mtor(mouse)-6×His-mCherry-Puro |
---|---|
别称: | NM_020009.2;2610315D21Rik; flat; FRAP; Frap1; FRAP2;RAFT1; RAPT1 |
基因长: | 7647bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 红色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-CHD1L(human)-6×His-mCherry-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-Mtor(mouse)-6×His-mCherry-Puro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动小鼠源Mtor基因转录,促进Mtor基因C端融合表达6×His标签,便于融合蛋白与镍离子发生亲和作用进行蛋白纯化,也可使用抗His抗体来检测和识别目的蛋白。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带mCherry红色荧光蛋白方便直观地检测目的基因的表达和定位情况。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
GAATTCGCCA
CCATGCTTGG
GACGGGTCCT
GCCGTGGCCA
CCGCCAGTGC
CGCCACATCT
AGCAACGTGA
GCGTCCTGCA
GCAGTTCGCC
AGTGGACTGA
AGAGCCGGAA
TGAGGAGACC
AGGGCCAAAG
CAGCCAAGGA
GCTCCAGCAC
TACGTCACCA
TGGAGCTTCG
AGAGATGAGT
CAGGAGGAGT
CTACTCGCTT
CTATGACCAG
CTGAACCATC
ACATTTTTGA
ACTGGTTTCC
AGCTCAGATG
CCAATGAGAG
GAAGGGTGGC
ATCTTGGCCA
TCGCCAGCCT
CATAGGAGTG
GAAGGTGGGA
ATTCCACCAG
AATTGGCAGA
TTTGCCAACT
ACCTTCGAAA
CCTCCTCCCC
TCAAGCGATC
CAGTTGTCAT
GGAAATGGCG
TCCAAGGCCA
TTGGCCGCCT
GGCGATGGCA
GGGGACACTT
TCACTGCTGA
ATATGTGGAG
TTTGAAGTGA
AGCGAGCCTT
GGAGTGGCTG
GGTGCTGACC
GAAATGAGGG
CCGGAGACAT
GCCGCTGTCC
TCGTTCTCCG
TGAGCTGGCC
ATCAGTGTCC
CCACCTTCTT
CTTCCAGCAA
GTTCAGCCCT
TCTTTGACAA
CATTTTTGTG
GCTGTGTGGG
ACCCCAAGCA
GGCCATCCGG
GAAGGCGCTG
TAGCGGCCCT
TCGTGCCTGT
CTGATTCTCA
CCACGCAGCG
GGAACCAAAG
GAAATGCAGA
AGCCTCAGTG
GTACCGGCAC
ACATTTGAAG
AAGCAGAGAA
AGGTTTTGAT
GAGACCCTGG
CCAAAGAGAA
GGGTATGAAT
CGAGATGATC
GAATCCACGG
AGCCTTGCTG
ATCCTCAACG
AGCTAGTTCG
TATCAGCAGC
ATGGAGGGAG
AGCGTCTGAG
AGAAGAGATG
GAGGAGATCA
CCCAGCAGCA
GCTGGTGCAT
GACAAGTACT
GCAAAGACCT
CATGGGCTTC
GGGACCAAGC
CTCGGCACAT
CACGCCCTTC
ACCAGTTTCC
AGGCTGTGCA
GCCCCAGCAG
CCGAACGCCT
TGGTGGGACT
GCTGGGGTAC
AGCTCCCCTC
AAGGCCTGAT
GGGATTTGGG
ACGTCCCCCA
GCCCTGCCAA
GTCCACTCTG
GTGGAAAGCC
GCTGTTGCAG
AGACTTGATG
GAAGAGAAAT
TTGATCAGGT
GTGCCAGTGG
GTGCTGAAGT
GCAGGAGCAG
CAAGAACTCG
CTGATCCAGA
TGACAATCCT
TAACCTGCTG
CCCCGCCTGG
CTGCATTCCG
ACCGTCCGCC
TTCACAGATA
CCCAGTACCT
CCAGGACACC
ATGAACCATG
TCCTGAGCTG
TGTCAAGAAG
GAGAAGGAAC
GGACTGCGGC
GTTCCAGGCC
CTGGGGCTGC
TTTCTGTGGC
CGTGAGGTCG
GAGTTTAAGG
TCTACTTGCC
CCGTGTACTT
GACATCATCC
GAGCAGCGCT
TCCTCCAAAG
GACTTTGCCC
ACAAGAGGCA
GAAAACCGTG
CAGGTGGATG
CCACCGTATT
CACGTGCATC
AGCATGTTGG
CACGAGCAAT
GGGGCCGGGC
ATCCAGCAGG
ACATCAAGGA
GCTGCTGGAG
CCCATGTTGG
CAGTGGGCCT
GAGCCCCGCG
CTCACTGCTG
TGCTCTATGA
CCTGAGCCGG
CAGATTCCAC
AGCTGAAGAA
AGATATTCAG
GACGGCCTTC
TGAAGATGCT
GTCCCTGGTC
CTTATGCACA
AACCCCTCCG
GCACCCAGGC
ATGCCCAAAG
GCCTGGCTCA
CCAGCTGGCT
TCCCCTGGTC
TCACCACCCT
CCCTGAGGCC
AGCGACGTGG
CCAGCATCAC
TCTTGCCCTT
CGAACCCTTG
GCAGCTTTGA
ATTTGAAGGC
CACTCTCTGA
CCCAGTTCGT
CCGACACTGC
GCAGATCACT
TCCTGAACAG
CGAGCACAAG
GAGATCCGCA
TGGAAGCTGC
TCGCACCTGC
TCCCGCCTGC
TCACACCCTC
CATCCACCTC
ATCAGCGGCC
ATGCCCACGT
GGTTAGCCAG
ACTGCAGTGC
AGGTGGTGGC
AGATGTGCTC
AGCAAGCTGC
TTGTGGTTGG
CATAACAGAT
CCTGACCCTG
ATATCCGCTA
CTGTGTCTTG
GCATCCCTGG
ACGAGCGCTT
TGATGCCCAC
CTGGCCCAGG
CAGAAAACTT
ACAAGCTCTG
TTTGTGGCTC
TGAATGACCA
GGTCTTTGAG
ATCCGCGAGC
TGGCCATCTG
CACTGTGGGC
CGACTAAGCA
GCATGAACCC
AGCCTTCGTC
ATGCCTTTCC
TGCGCAAGAT
GCTCATCCAG
ATCCTGACAG
AGCTGGAGCA
CAGCGGCATT
GGGAGAATCA
AGGAGCAGAG
CGCCCGCATG
CTGGGGCACC
TGGTGTCCAA
CGCCCCCCGG
CTCATCCGCC
CCTACATGGA
GCCTATCCTG
AAGGCTTTAA
TTTTGAAACT
GAAAGATCCA
GACCCTGACC
CAAACCCGGG
CGTGATCAAT
AACGTGTTGG
CCACTATAGG
AGAACTGGCT
CAGGTGAGCG
GCCTGGAAAT
GCGGAAGTGG
GTGGACGAGC
TCTTTATCAT
CATCATGGAC
ATGCTGCAGG
ACTCCTCCCT
GCTGGCCAAA
AGGCAGGTGG
CTTTGTGGAC
CCTGGGACAG
TTGGTGGCCA
GCACTGGCTA
TGTGGTGGAG
CCCTACAGGA
AGTACCCCAC
TTTGCTTGAA
GTGCTGCTGA
ATTTCCTGAA
GACGGAGCAG
AACCAGGGCA
CTCGGAGAGA
GGCTATCCGA
GTGTTGGGGC
TCCTTGGGGC
TTTGGATCCC
TACAAGCACA
AAGTGAACAT
CGGCATGATC
GACCAGTCCC
GGGACGCTTC
CGCTGTCAGC
CTGTCAGAGT
CCAAGTCAAG
TCAGGATTCC
TCTGACTACA
GCACCAGTGA
AATGCTGGTC
AACATGGGAA
ACCTGCCCCT
GGACGAGTTC
TACCCCGCTG
TGTCCATGGT
GGCCTTGATG
CGGATCTTCC
GAGATCAATC
CCTCTCTCAC
CACCACACCA
TGGTGGTGCA
GGCCATCACC
TTCATCTTCA
AGTCCCTGGG
GCTCAAGTGT
GTGCAGTTCC
TGCCCCAGGT
CATGCCCACA
TTCCTTAATG
TCATCCGAGT
CTGTGATGGG
GCCATCCGGG
AATTTCTGTT
CCAGCAGCTG
GGGATGCTGG
TGTCCTTTGT
GAAGAGCCAC
ATCCGTCCCT
ACATGGATGA
AATAGTCACT
CTCATGAGAG
AGTTTTGGGT
CATGAACACG
TCAATCCAGA
GCACAATCAT
TCTTCTCATT
GAGCAGATTG
TGGTGGCTCT
CGGAGGGGAA
TTTAAGCTTT
ATCTGCCCCA
GTTGATCCCA
CACATGCTGC
GGGTCTTCAT
GCATGACAAC
AGCCAAGGCC
GAATCGTCTC
CATCAAGCTG
TTAGCCGCGA
TCCAGCTGTT
TGGCGCCAAC
CTGGATGACT
ATCTGCACTT
GTTGTTGCCT
CCGATTGTGA
AATTGTTTGA
TGCCCCTGAA
GTCCCGCTGC
CATCAAGAAA
GGCAGCGCTG
GAGACGGTGG
ACCGCCTGAC
AGAGTCCCTA
GACTTCACTG
ACTACGCCTC
CCGCATCATT
CACCCAATAG
TTCGTACGCT
AGACCAGAGC
CCAGAGCTGC
GCTCCACAGC
CATGGACACT
CTGTCTTCGC
TTGTCTTTCA
ACTGGGGAAG
AAGTACCAGA
TCTTCATTCC
AATGGTGAAT
AAAGTCCTCG
TGCGACACCG
GATCAACCAC
CAGCGCTATG
ATGTGCTTAT
CTGCAGAATC
GTCAAGGGGT
ACACACTTGC
TGATGAGGAA
GAAGACCCTT
TGATTTACCA
GCATCGAATG
CTAAGGAGCA
GCCAGGGAGA
TGCCCTGGCC
AGTGGACCAG
TTGAGACAGG
ACCCATGAAG
AAACTGCATG
TCAGCACCAT
CAACCTCCAA
AAGGCCTGGG
GAGCTGCCAG
AAGGGTCTCC
AAGGACGACT
GGCTGGAGTG
GCTGAGGCGC
TTGAGTCTGG
AGCTTCTGAA
GGACTCCTCA
TCGCCCTCCC
TGCGCTCATG
CTGGGCCCTG
GCGCAGGCCT
ACAACCCCAT
GGCCAGGGAT
CTCTTCAATG
CTGCCTTTGT
GTCCTGCTGG
TCTGAGCTGA
ATGAAGACCA
GCAAGATGAG
CTCATCAGGA
GTATTGAGTT
GGCTCTCACT
TCTCAAGACA
TTGCTGAAGT
CACACAAACC
CTCCTGAACT
TGGCTGAGTT
CATGGAACAC
AGTGACAAGG
GCCCCCTGCC
GCTGAGAGAT
GACAATGGCA
TCGTGCTCCT
GGGTGAGAGA
GCTGCCAAGT
GCCGGGCATA
TGCCAAAGCA
CTGCACTACA
AAGAACTGGA
GTTCCAGAAA
GGGCCCACGC
CTGCCATACT
TGAGTCCCTC
ATCAGCATTA
ACAACAAGCT
CCAGCAGCCT
GAGGCAGCTT
CTGGGGTGTT
GGAATACGCC
ATGAAACACT
TCGGAGAGCT
GGAGATCCAG
GCCACCTGGT
ATGAGAAGCT
GCATGAGTGG
GAGGATGCTC
TCGTGGCCTA
CGACAAGAAG
ATGGACACAA
ACAAGGAAGA
CCCGGAGCTG
ATGCTGGGCC
GAATGCGCTG
CCTCGAGGCC
TTGGGGGAAT
GGGGCCAGCT
TCATCAGCAG
TGCTGTGAAA
AGTGGACTCT
GGTTAATGAT
GAGACCCAGG
CTAAGATGGC
CCGGATGGCT
GCTGCTGCAG
CGTGGGGTTT
AGGTCAGTGG
GACAGCATGG
AGGAGTACAC
CTGCATGATC
CCACGGGACA
CCCACGATGG
AGCCTTTTAC
AGGGCAGTGT
TGGCTCTACA
TCAGGATCTC
TTCTCCTTGG
CCCAGCAGTG
CATTGACAAG
GCCAGGGACC
TGCTGGATGC
AGAGCTGACT
GCCATGGCAG
GAGAGAGCTA
CAGCCGAGCC
TATGGGGCCA
TGGTTTCTTG
CCACATGCTG
TCCGAGCTGG
AAGAGGTTAT
CCAGTACAAA
CTTGTCCCTG
AGCGTCGGGA
GATCATCCGG
CAGATCTGGT
GGGAGAGACT
GCAGGGCTGC
CAGCGTATTG
TTGAGGACTG
GCAGAAAATC
CTCATGGTCC
GGTCCCTTGT
GGTCAGCCCT
CATGAGGACA
TGAGAACCTG
GCTCAAGTAC
GCAAGCCTGT
GTGGCAAGAG
TGGCAGACTG
GCTCTTGCTC
ATAAAACCTT
AGTGTTGCTC
TTGGGAGTTG
ATCCATCTCG
GCAACTTGAC
CATCCTCTGC
CAACCGCTCA
CCCTCAAGTG
ACCTATGCCT
ACATGAAGAA
CATGTGGAAA
AGTGCTCGGA
AGATTGACGC
CTTCCAGCAC
ATGCAACACT
TTGTGCAGAC
CATGCAGCAG
CAGGCCCAGC
ATGCCATCGC
CACAGAGGAC
CAGCAGCACA
AGCAGGAGCT
GCATAAGCTC
ATGGCCAGGT
GTTTTCTGAA
ACTTGGGGAG
TGGCAGCTGA
ACCTCCAGGG
CATCAACGAG
AGCACCATCC
CCAAGGTGCT
ACAGTACTAC
AGTGCCGCCA
CAGAGCATGA
CCGCAGCTGG
TACAAGGCTT
GGCATGCATG
GGCAGTGATG
AACTTCGAAG
CAGTGCTACA
CTACAAACAT
CAGAACCAAG
CCCGTGATGA
GAAGAAGAAG
CTGCGTCATG
CCAGCGGGGC
CAACATCACC
AATGCCACCA
CTGCAGCCAC
CACTGCAGCC
TCTGCTGCTG
CTGCCACCAG
CACAGAGGGC
AGCAACAGTG
AGAGTGAAGC
TGAGAGCAAT
GAGAACAGCC
CCACCCCGTC
CCCTCTGCAG
AAGAAGGTCA
CTGAGGATTT
ATCCAAAACT
CTCTTGTTGT
ACACTGTCCC
TGCTGTTCAA
GGCTTCTTCC
GTTCTATCTC
CTTGTCAAGA
GGCAACAACC
TCCAGGATAC
ACTAAGAGTC
CTCACCCTGT
GGTTTGATTA
TGGTCACTGG
CCAGATGTCA
ATGAAGCCTT
GGTGGAAGGG
GTGAAGGCCA
TACAGATTGA
CACTTGGTTA
CAGGTTATAC
CTCAGCTCAT
TGCAAGAATT
GACACACCCA
GACCCTTGGT
GGGCCGGCTC
ATTCACCAGC
TTCTCACAGA
TATTGGTCGG
TACCACCCAC
AGGCCCTCAT
CTACCCCCTG
ACGGTGGCTT
CTAAGTCTAC
CACCACAGCC
CGTCACAATG
CAGCCAACAA
GATCTTGAAG
AACATGTGTG
AACACAGCAA
CACGCTGGTC
CAGCAGGCCA
TGATGGTGAG
TGAAGAGCTG
ATTCGGGTAG
CCATCCTCTG
GCATGAGATG
TGGCATGAAG
GCCTGGAAGA
GGCATCTCGC
TTGTACTTTG
GGGAGAGGAA
CGTGAAAGGC
ATGTTTGAGG
TGCTGGAGCC
CCTGCATGCT
ATGATGGAAC
GGGGTCCCCA
GACTCTGAAG
GAAACATCCT
TTAATCAGGC
ATATGGCCGA
GATTTAATGG
AGGCACAAGA
ATGGTGTCGA
AAGTACATGA
AGTCGGGGAA
CGTCAAGGAC
CTCACGCAAG
CCTGGGACCT
CTACTATCAC
GTGTTCAGAC
GGATCTCAAA
GCAGCTACCC
CAGCTCACAT
CCCTGGAGCT
GCAGTATGTG
TCCCCCAAAC
TTCTGATGTG
CCGAGACCTT
GAGTTGGCTG
TGCCAGGAAC
ATACGACCCC
AACCAGCCAA
TCATTCGCAT
TCAATCCATA
GCCCCGTCTT
TGCAAGTCAT
CACATCCAAG
CAGAGGCCTC
GGAAGCTGAC
TCTGATGGGC
AGCAATGGGC
ATGAGTTTGT
TTTCCTCCTG
AAAGGCCATG
AAGATCTGCG
GCAGGATGAA
CGAGTGATGC
AGCTCTTTGG
CCTGGTGAAC
ACACTCCTAG
CCAATGACCC
CACTTCTCTT
CGCAAGAACC
TCAGCATCCA
GAGATACGCT
GTCATCCCTC
TGTCCACCAA
CTCGGGCCTC
ATTGGCTGGG
TGCCCCACTG
TGACACACTG
CATGCCCTCA
TCCGGGACTA
CAGAGAGAAG
AAGAAGATCC
TGTTGAACAT
CGAGCATCGC
ATCATGCTGC
GGATGGCTCC
TGACTATGAC
CACCTGACGT
TGATGCAGAA
GGTAGAGGTG
TTTGAGCATG
CTGTCAACAA
CACAGCTGGG
GACGACCTGG
CCAAGCTACT
GTGGCTAAAA
AGCCCCAGCT
CGGAGGTGTG
GTTTGACCGA
AGAACCAACT
ATACCCGCTC
CCTGGCCGTT
ATGTCGATGG
TCGGATACAT
TTTAGGCCTT
GGAGACAGGC
ACCCATCCAA
TCTGATGCTG
GACCGGCTGA
GTGGGAAGAT
CCTGCACATT
GACTTTGGGG
ACTGCTTTGA
GGTCGCTATG
ACCAGAGAGA
AATTTCCAGA
AAAGATTCCA
TTTAGACTAA
CAAGAATGTT
GACCAATGCT
ATGGAGGTTA
CGGGTCTGGA
TGGCAACTAC
AGAACCACAT
GCCACACCGT
GATGGAAGTG
CTCCGGGAAC
ACAAGGACAG
TGTCATGGCT
GTGCTGGAAG
CCTTTGTCTA
TGACCCACTG
CTCAACTGGA
GGCTGATGGA
CACAAATACC
AAAGGCAATA
AGCGGTCCCG
GACAAGGACA
GACTCCTACT
CTGCCGGCCA
GTCAGTAGAA
ATTTTGGACG
GTGTAGAACT
TGGAGAACCA
GCCCATAAGA
AAGCAGGGAC
CACTGTGCCA
GAATCCATCC
ATTCATTCAT
TGGAGACGGT
TTGGTGAAAC
CAGAAGCCTT
AAACAAGAAA
GCTATTCAGA
TTATTAACAG
GGTTCGAGAT
AAGCTCACTG
GTCGGGATTT
CTCTCATGAT
GACACTTTGG
ATGTTCCAAC
CCAAGTGGAG
CTGCTTATCA
AGCAGGCAAC
ATCTCACGAG
AACCTCTGCC
AGTGCTACAT
TGGCTGGTGT
CCCTTCTGGG
GATCCCATCA
TCATCATCAT
CACTAGCGGC
CGCGAAGGAT
CTGCGATCGC
TCCGGTGCCC
GTCAGTGGGC
AGAGCGCACA
TCGCCCACAG
TCCCCGAGAA
GTTGGGGGGA
GGGGTCGGCA
ATTGAACGGG
TGCCTAGAGA
AGGTGGCGCG
GGGTAAACTG
GGAAAGTGAT
GTCGTGTACT
GGCTCCGCCT
TTTTCCCGAG
GGTGGGGGAG
AACCGTATAT
AAGTGCAGTA
GTCGCCGTGA
ACGTTCTTTT
TCGCAACGGG
TTTGCCGCCA
GAACACAGCT
GAAGCTTCGA
GGGGCTCGCA
TCTCTCCTTC
ACGCGCCCGC
CGCCCTACCT
GAGGCCGCCA
TCCACGCCGG
TTGAGTCGCG
TTCTGCCGCC
TCCCGCCTGT
GGTGCCTCCT
GAACTGCGTC
CGCCGTCTAG
GTAAGTTTAA
AGCTCAGGTC
GAGACCGGGC
CTTTGTCCGG
CGCTCCCTTG
GAGCCTACCT
AGACTCAGCC
GGCTCTCCAC
GCTTTGCCTG
ACCCTGCTTG
CTCAACTCTA
CGTCTTTGTT
TCGTTTTCTG
TTCTGCGCCG
TTACAGATCC
AAGCTGTGAC
CGGCGCCTAC
GCTAGACGCC
ACCATGGTGA
GCAAGGGCGA
GGAGGATAAC
ATGGCCATCA
TCAAGGAGTT
CATGCGCTTC
AAGGTGCACA
TGGAGGGCTC
CGTGAACGGC
CACGAGTTCG
AGATCGAGGG
CGAGGGCGAG
GGCCGCCCCT
ACGAGGGCAC
CCAGACCGCC
AAGCTGAAGG
TGACCAAGGG
TGGCCCCCTG
CCCTTCGCCT
GGGACATCCT
GTCCCCTCAG
TTCATGTACG
GCTCCAAGGC
CTACGTGAAG
CACCCCGCCG
ACATCCCCGA
CTACTTGAAG
CTGTCCTTCC
CCGAGGGCTT
CAAGTGGGAG
CGCGTGATGA
ACTTCGAGGA
CGGCGGCGTG
GTGACCGTGA
CCCAGGACTC
CTCCCTGCAG
GACGGCGAGT
TCATCTACAA
GGTGAAGCTG
CGCGGCACCA
ACTTCCCCTC
CGACGGCCCC
GTAATGCAGA
AGAAGACCAT
GGGCTGGGAG
GCCTCCTCCG
AGCGGATGTA
CCCCGAGGAC
GGCGCCCTGA
AGGGCGAGAT
CAAGCAGAGG
CTGAAGCTGA
AGGACGGCGG
CCACTACGAC
GCTGAGGTCA
AGACCACCTA
CAAGGCCAAG
AAGCCCGTGC
AGCTGCCCGG
CGCCTACAAC
GTCAACATCA
AGTTGGACAT
CACCTCCCAC
AACGAGGACT
ACACCATCGT
GGAACAGTAC
GAACGCGCCG
AGGGCCGCCA
CTCCACCGGC
GGCATGGACG
AGCTGTACAA
GGGATCTCGC
GAGGGCAGAG
GAAGTCTTCT
AACATGCGGT
GACGTGGAGG
AGAATCCCGG
CCCTATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCAGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGATCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
CGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CTGAAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAG
CTGACGTCCT
TTCCATGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTCCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
CCGCCTCCCC
GCCTGGTACC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAAATAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TAGTAGTTCA
TGTCATCTTA
TTATTCAGTA
TTTATAACTT
GCAAAGAAAT
GAATATCAGA
GAGTGAGAGG
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGCTCTAGCT
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCG
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGA
CTTTTGCAGA
GACGGCCCAA
ATTCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCATACTCT
TCCTTTTTCA
ATATTATTGA
AGCATTTATC
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGACGT
CTAAGAAACC
ATTATTATCA
TGACATTAAC
CTATAAAAAT
AGGCGTATCA
CGAGGCCCTT
TCGTCTCGCG
CGTTTCGGTG
ATGACGGTGA
AAACCTCTGA
CACATGCAGC
TCCCGGAGAC
GGTCACAGCT
TGTCTGTAAG
CGGATGCCGG
GAGCAGACAA
GCCCGTCAGG
GCGCGTCAGC
GGGTGTTGGC
GGGTGTCGGG
GCTGGCTTAA
CTATGCGGCA
TCAGAGCAGA
TTGTACTGAG
AGTGCACCAT
ATGCGGTGTG
AAATACCGCA
CAGATGCGTA
AGGAGAAAAT
ACCGCATCAG
GCGCCATTCG
CCATTCAGGC
TGCGCAACTG
TTGGGAAGGG
CGATCGGTGC
GGGCCTCTTC
GCTATTACGC
CAGCTGGCGA
AAGGGGGATG
TGCTGCAAGG
CGATTAAGTT
GGGTAACGCC
AGGGTTTTCC
CAGTCACGAC
GTTGTAAAAC
GACGGCCAGT
GCCAAGCTG