名称: | pHAGE-PGK-PELI1-BirA-IRES-Nluc-copGFP |
---|---|
别称: | - |
基因长: | 1252bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色,Nluc |
启动子: | PGK |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pHAGE PGK-COPRS-FLag_NES-IRES-Nluc-copGFP-W |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pHAGE-PGK-PELI1-BirA-IRES-Nluc-copGFP是适用于哺乳细胞过表达的质粒。质粒带有的PGK启动子可启动下游PELI1基因的转录,PELI1是 E3 泛素蛋白连接酶 PELI1 的基因序列,参与细胞内蛋白质的泛素化修饰过程,BirA是一种生物素连接酶,便于后续通过与链霉亲和素等的特异性结合进行蛋白质的检测、纯化等操作。质粒携带的CopGFP绿色荧光蛋白和Nluc荧光素酶用于观察目的基因的表达和定位情况。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCGAA
TTCACAAATG
GCAGTATTCA
TCCACAATTT
TAAAAGAAAA
GGGGGGATTG
GGGGGTACAG
TGCAGGGGAA
AGAATAGTAG
ACATAATAGC
AACAGACATA
CAAACTAAAG
AATTACAAAA
ACAAATTACA
AAAATTCAAA
ATTTTCGGGT
TTATTACAGG
GACAGCAGAG
ATCCAGTTTG
GACTAGCATT
CTACCGGGTA
GGGGAGGCGC
TTTTCCCAAG
GCAGTCTGGA
GCATGCGCTT
TAGCAGCCCC
GCTGGGCACT
TGGCGCTACA
CAAGTGGCCT
CTGGCCTCGC
ACACATTCCA
CATCCACCGG
TAGGCGCCAA
CCGGCTCCGT
TCTTTGGTGG
CCCCTTCGCG
CCACCTTCTA
CTCCTCCCCT
AGTCAGGAAG
TTCCCCCCCG
CCCCGCAGCT
CGCGTCGTGC
AGGACGTGAC
AAATGGAAGT
AGCACGTCTC
ACTAGTCTCG
TGCAGATGGA
CAGCACCGCT
GAGCAATGGA
AGCGGGTAGG
CCTTTGGGGC
AGCGGCCAAT
AGCAGCTTTG
CTCCTTCGCT
TTCTGGGCTC
AGGGGCGGGG
CGGGCGCCCG
AAGGTCCTCC
GGAGGCCCGG
CATTCTGCAC
GCTTCAAAAG
CGCACGTCTG
CCGCGCTGTT
CTCCTCTTCC
TCATCTCCGG
GCCTTTCGAC
CTGCGGCCGC
ATGTTTTCTC
CTGATCAAGA
AAATCATCCA
TCTAAAGCAC
CAGTAAAATA
TGGTGAACTC
ATTGTCTTAG
GGTATAATGG
GTCTCTCCCA
AATGGCGATA
GAGGAAGGAG
GAAAAGTAGG
TTTGCTTTGT
TTAAAAGACC
TAAGGCAAAT
GGGGTGAAGC
CCAGCACTGT
GCATATTGCT
TGTACTCCTC
AGGCTGCAAA
GGCAATAAGC
AACAAAGACC
AGCATAGCAT
ATCATATACT
TTATCTCGGG
CCCAGACTGT
GGTGGTTGAA
TATACTCATG
ACAGCAACAC
CGATATGTTT
CAGATTGGCC
GGTCGACTGA
AAGCCCCATT
GATTTTGTAG
TAACTGACAC
GGTTCCTGGA
AGTCAAAGTA
ATTCTGATAC
ACAGTCAGTA
CAAAGCACTA
TATCAAGATT
TGCCTGCAGA
ATCATATGTG
AACGGAATCC
TCCCTTTACA
GCACGGATTT
ATGCTGCAGG
ATTTGACTCA
TCAAAAAACA
TCTTTCTTGG
GGAGAAGGCT
GCCAAATGGA
AGACATCAGA
TGGACAGATG
GATGGCTTGA
CCACTAATGG
TGTTCTTGTG
ATGCATCCAC
GCAATGGGTT
CACAGAAGAC
TCCAAGCCTG
GAATATGGAG
AGAAATATCG
GTGTGTGGAA
ATGTATTTAG
CCTACGTGAA
ACCAGATCGG
CTCAGCAGAG
AGGAAAAATG
GTGGAAATTG
AAACCAATCA
GTTACAAGAT
GGCTCGTTAA
TTGACCTCTG
TGGTGCAACA
TTGTTATGGC
GTACTGCAGA
AGGCCTTTCC
CACACTCCTA
CCGTGAAGCA
TTTAGAAGCT
TTAAGACAGG
AAATCAATGC
AGCACGACCT
CAGTGCCCTG
TAGGGTTCAA
CACACTAGCA
TTTCCTAGTA
TGAAGAGGAA
AGACGTTGTA
GATGAAAAAC
AACCATGGGT
ATATCTAAAC
TGCGGCCATG
TACATGGCTA
TCATAACTGG
GGAAACAAAG
AAGAACGTGA
TGGAAAAGAT
CGTGAATGTC
CTATGTGTAG
GTCTGTTGGT
CCCTATGTTC
CTCTGTGGCT
TGGATGTGAA
GCTGGATTTT
ATGTGGACGC
CGGCCCTCCA
ACCCATGCGT
TTAGCCCGTG
TGGGCATGTG
TGTTCAGAAA
AGACAACTGC
CTATTGGTCC
CAGATCCCAC
TTCCTCATGG
TACTCATACT
TTTCATGCAG
CCTGTCCCTT
TTGTGCACAT
CAGTTGGCTG
GTGAACAAGG
CTACATCAGA
CTTATTTTTC
AAGGACCTCT
AGACGACTAC
AAAGACCATG
ACGGTGATTA
TAAAGATCAT
GACATCGATT
ACAAGGATGA
CGATGACAAG
CTTATGAAGG
ATAACACCGT
GCCACTGAAA
TTGATTGCCC
TGTTAGCGAA
CGGTGAATTT
CACTCTGGCG
AGCAGTTGGG
TGAAACGCTG
GGAATGAGCC
GGGCGGCTAT
TAATAAACAC
ATTCAGACAC
TGCGTGACTG
GGGCGTTGAT
GTCTTTACCG
TTCCGGGTAA
AGGATACAGC
CTGCCTGAGC
CTATCCAGTT
ACTTAATGCT
AAACAGATAT
TGGGTCAGCT
GGATGGCGGT
AGTGTAGCCG
TGCTGCCAGT
GATTGACTCC
ACGAATCAGT
ACCTTCTTGA
TCGTATCGGA
GAGCTTAAAT
CGGGCGATGC
TTGCATTGCA
GAATACCAGC
AGGCTGGCCG
TGGTCGCCGG
GGTCGGAAAT
GGTTTTCGCC
TTTTGGCGCA
AACTTATATT
TGTCGATGTT
CTGGCGTCTG
GAACAAGGCC
CGGCGGCGGC
GATTGGTTTA
AGTCTGGTTA
TCGGTATCGT
GATGGCGGAA
GTATTACGCA
AGCTGGGTGC
AGATAAAGTT
CGTGTTAAAT
GGCCTAATGA
CCTCTATCTG
CAGGATCGCA
AGCTGGCAGG
CATTCTGGTG
GAGCTGACTG
GCAAAACTGG
CGATGCGGCG
CAAATAGTCA
TTGGAGCCGG
GATCAACATG
GCAATGCGCC
GTGTTGAAGA
GAGTGTCGTT
AATCAGGGGT
GGATCACGCT
GCAGGAAGCG
GGGATCAATC
TCGATCGTAA
TACGTTGGCG
GCCATGCTAA
TACGTGAATT
ACGTGCTGCG
TTGGAACTCT
TCGAACAAGA
AGGATTGGCA
CCTTATCTGT
CGCGCTGGGA
AAAGCTGGAT
AATTTTATTA
ATCGCCCAGT
GAAACTTATC
ATTGGTGATA
AAGAAATATT
TGGCATTTCA
CGCGGAATAG
ACAAACAGGG
GGCTTTATTA
CTTGAGCAGG
ATGGAATAAT
AAAACCCTGG
ATGGGCGGTG
AAATATCCCT
GCGTAGTGCA
GAAAAATGAG
GATCCCTCCC
CCCCCCCTAA
CGTTACTGGC
CGAAGCCGCT
TGGAATAAGG
CCGGTGTGCG
TTTGTCTATA
TGTTATTTTC
CACCATATTG
CCGTCTTTTG
GCAATGTGAG
GGCCCGGAAA
CCTGGCCCTG
TCTTCTTGAC
GAGCATTCCT
AGGGGTCTTT
CCCCTCTCGC
CAAAGGAATG
CAAGGTCTGT
TGAATGTCGT
GAAGGAAGCA
GTTCCTCTGG
AAGCTTCTTG
AAGACAAACA
ACGTCTGTAG
CGACCCTTTG
CAGGCAGCGG
AACCCCCCAC
CTGGCGACAG
GTGCCTCTGC
GGCCAAAAGC
CACGTGTATA
AGATACACCT
GCAAAGGCGG
CACAACCCCA
GTGCCACGTT
GTGAGTTGGA
TAGTTGTGGA
AAGAGTCAAA
TGGCTCTCCT
CAAGCGTATT
CAACAAGGGG
CTGAAGGATG
CCCAGAAGGT
ACCCCATTGT
ATGGGATCTG
ATCTGGGGCC
TCGGTGCACA
TGCTTTACAT
GTGTTTAGTC
GAGGTTAAAA
AAACGTCTAG
GCCCCCCGAA
CCACGGGGAC
GTGGTTTTCC
TTTGAAAAAC
ACGATGATAA
TATGGCCACA
CATATGGTCT
TCACACTCGA
AGATTTCGTT
GGGGACTGGC
GACAGACAGC
CGGCTACAAC
CTGGACCAAG
TCCTTGAACA
GGGAGGTGTG
TCCAGTTTGT
TTCAGAATCT
CGGGGTGTCC
GTAACTCCGA
TCCAAAGGAT
TGTCCTGAGC
GGTGAAAATG
GGCTGAAGAT
CGACATCCAT
GTCATCATCC
CGTATGAAGG
TCTGAGCGGC
GACCAAATGG
GCCAGATCGA
AAAAATTTTT
AAGGTGGTGT
ACCCTGTGGA
TGATCATCAC
TTTAAGGTGA
TCCTGCACTA
TGGCACACTG
GTAATCGACG
GGGTTACGCC
GAACATGATC
GACTATTTCG
GACGGCCGTA
TGAAGGCATC
GCCGTGTTCG
ACGGCAAAAA
GATCACTGTA
ACAGGGACCC
TGTGGAACGG
CAACAAAATT
ATCGACGAGC
GCCTGATCAA
CCCCGACGGC
TCCCTGCTGT
TCCGAGTAAC
CATCAACGGA
GTGACCGGCT
GGCGGCTGTG
CGAACGCATT
CTGGCGAAGC
TTGGAAGCGG
AGCTACTAAC
TTCAGCCTGC
TGAAGCAGGC
TGGAGACGTG
GAGGAGAACC
CTGGACCTAT
GGAGAGCGAC
GAGAGCGGCC
TGCCCGCCAT
GGAGATCGAG
TGCCGCATCA
CCGGCACCCT
GAACGGCGTG
GAGTTCGAGC
TGGTGGGCGG
CGGAGAGGGC
ACCCCCAAGC
AGGGCCGCAT
GACCAACAAG
ATGAAGAGCA
CCAAAGGCGC
CCTGACCTTC
AGCCCCTACC
TGCTGAGCCA
CGTGATGGGC
TACGGCTTCT
ACCACTTCGG
CACCTACCCC
AGCGGCTACG
AGAACCCCTT
CCTGCACGCC
ATCAACAACG
GCGGCTACAC
CAACACCCGC
ATCGAGAAGT
ACGAGGACGG
CGGCGTGCTG
CACGTGAGCT
TCAGCTACCG
CTACGAGGCC
GGCCGCGTGA
TCGGCGACTT
CAAGGTGGTG
GGCACCGGCT
TCCCCGAGGA
CAGCGTGATC
TTCACCGACA
AGATCATCCG
CAGCAACGCC
ACCGTGGAGC
ACCTGCACCC
CATGGGCGAT
AACGTGCTGG
TGGGCAGCTT
CGCCCGCACC
TTCAGCCTGC
GCGACGGCGG
CTACTACAGC
TTCGTGGTGG
ACAGCCACAT
GCACTTCAAG
AGCGCCATCC
ACCCCAGCAT
CCTGCAGAAC
GGGGGCCCCA
TGTTCGCCTT
CCGCCGCGTG
GAGGAGCTGC
ACAGCAACAC
CGAGCTGGGC
ATCGTGGAGT
ACCAGCACGC
CTTCAAGACC
CCCATTGCCT
TCGCCATCGA
TAGATCCTAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAATCATCG
TCCTTTCCTT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTTCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGGCCGCC
TCCCCGCCTG
AGATCCTTTA
AGACCAATGA
CTTACAAGGC
AGCTGTAGAT
CTTAGCCACT
TTTTAAAAGA
AAAGGGGGGA
CTGGAAGGGC
TAATTCACTC
CCAACGAAGA
CAAGATCTGC
TTTTTGCTTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTAGT
AGTTCATGTC
ATCTTATTAT
TCAGTATTTA
TAACTTGCAA
AGAAATGAAT
ATCAGAGAGT
GAGAGGCCCG
GGTTAATTAA
GGAAAGGGCT
AGATCATTCT
TGAAGACGAA
AGGGCCTCGT
GATACGCCTA
TTTTTATAGG
TTAATGTCAT
GATAATAATG
GTTTCTTAGA
CGTCAGGTGG
CACTTTTCGG
GGAAATGTGC
GCGGAACCCC
TATTTGTTTA
TTTTTCTAAA
TACATTCAAA
TATGTATCCG
CTCATGAGAC
AATAACCCTG
ATAAATGCTT
CAATAATATT
GAAAAAGGAA
GAGTATGAGT
ATTCAACATT
TCCGTGTCGC
CCTTATTCCC
TTTTTTGCGG
CATTTTGCCT
TCCTGTTTTT
GCTCACCCAG
AAACGCTGGT
GAAAGTAAAA
GATGCTGAAG
ATCAGTTGGG
TGCACGAGTG
GGTTACATCG
AACTGGATCT
CAACAGCGGT
AAGATCCTTG
AGAGTTTTCG
CCCCGAAGAA
CGTTTTCCAA
TGATGAGCAC
TTTTAAAGTT
CTGCTATGTG
GCGCGGTATT
ATCCCGTGTT
GACGCCGGGC
AAGAGCAACT
CGGTCGCCGC
ATACACTATT
CTCAGAATGA
CTTGGTTGAG
TACTCACCAG
TCACAGAAAA
GCATCTTACG
GATGGCATGA
CAGTAAGAGA
ATTATGCAGT
GCTGCCATAA
CCATGAGTGA
TAACACTGCG
GCCAACTTAC
TTCTGACAAC
GATCGGAGGA
CCGAAGGAGC
TAACCGCTTT
TTTGCACAAC
ATGGGGGATC
ATGTAACTCG
CCTTGATCGT
TGGGAACCGG
AGCTGAATGA
AGCCATACCA
AACGACGAGC
GTGACACCAC
GATGCCTGTA
GCAATGGCAA
CAACGTTGCG
CAAACTATTA
ACTGGCGAAC
TACTTACTCT
AGCTTCCCGG
CAACAATTAA
TAGACTGGAT
GGAGGCGGAT
AAAGTTGCAG
GACCACTTCT
GCGCTCGGCC
CTTCCGGCTG
GCTGGTTTAT
TGCTGATAAA
TCTGGAGCCG
GTGAGCGTGG
GTCTCGCGGT
ATCATTGCAG
CACTGGGGCC
AGATGGTAAG
CCCTCCCGTA
TCGTAGTTAT
CTACACGACG
GGGAGTCAGG
CAACTATGGA
TGAACGAAAT
AGACAGATCG
CTGAGATAGG
TGCCTCACTG
ATTAAGCATT
GGTAACTGTC
AGACCAAGTT
TACTCATATA
TACTTTAGAT
TGATTTAAAA
CTTCATTTTT
AATTTAAAAG
GATCTAGGTG
AAGATCCTTT
TTGATAATCT
CATGACCAAA
ATCCCTTAAC
GTGAGTTTTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTTCTTCTAG
TGTAGCCGTA
GTTAGGCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGTTCTTT
CCTGCGTTAT
CCCCTGATTC
TGTGGATAAC
CGTATTACCG
CCTTTGAGTG
AGCTGATACC
GCTCGCCGCA
GCCGAACGAC
CGAGCGCAGC
GAGTCAGTGA
GCGAGGAAGC
GGAAGAGCGC
CCAATACGCA
AACCGCCTCT
CCCCGCGCGT
TGGCCGATTC
ATTAATGCAG
CAAGCTCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAAAGCTC
CCCGTGGCAC
GACAGGTTTC
CCGACTGGAA
AGCGGGCAGT
GAGCGCAACG
CAATTAATGT
GAGTTAGCTC
ACTCATTAGG
CACCCCAGGC
TTTACACTTT
ATGCTTCCGG
CTCGTATGTT
GTGTGGAATT
GTGAGCGGAT
AACAATTTCA
CACAGGAAAC
AGCTATGACA
TGATTACGAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGA
TCAACTGGAT
AACTCAAGCT
AACCAAAATC
ATCCCAAACT
TCCCACCCCA
TACCCTATTA
CCACTGCCAA
TTACCTGTGG
TTTCATTTAC
TCTAAACCTG
TGATTCCTCT
GAATTATTTT
CATTTTAAAG
AAATTGTATT
TGTTAAATAT
GTACTACAAA
CTTAGTAGT