产品货号：P72221
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV3-CMV-IQGAP1(human)-3×Myc-Blast |
|---|---|
| 别称: | NM_003870.4;HUMORFA01; p195; SAR1; |
| 基因长: | 4971bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Blast |
| 荧光标记： | |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLV3-CMV-MCS-3×Myc-Blast |
| 正向引物: | CMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-IQGAP1(human)-3×Myc-Blast适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动人源IQGAP1基因转录，促进IQGAP1基因C端融合表达3×Myc标签，便于抗-Myc抗体在免疫印迹、免疫沉淀或免疫荧光等实验中识别和追踪带有Myc标签的目的蛋白，从而研究目的蛋白的表达水平、亚细胞定位以及与其他蛋白的相互作用等。此外，质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装，便于基因稳定整合至宿主细胞。质粒携带Blast抗性基因，用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCTAGCG
AATTCGCCAC
CATGTCCGCC
GCAGACGAGG
TTGACGGGCT
GGGCGTGGCC
CGGCCGCACT
ATGGCTCTGT
CCTGGATAAT
GAAAGACTTA
CTGCAGAGGA
GATGGATGAA
AGGAGACGTC
AGAACGTGGC
TTATGAGTAC
CTTTGTCATT
TGGAAGAAGC
GAAGAGGTGG
ATGGAAGCAT
GCCTAGGGGA
AGATCTGCCT
CCCACCACAG
AACTGGAGGA
GGGGCTTAGG
AATGGGGTCT
ACCTTGCCAA
ACTGGGGAAC
TTCTTCTCTC
CCAAAGTAGT
GTCCCTGAAA
AAAATCTATG
ATCGAGAACA
GACCAGATAC
AAGGCGACTG
GCCTCCACTT
TAGACACACT
GATAATGTGA
TTCAGTGGTT
GAATGCCATG
GATGAGATTG
GATTGCCTAA
GATTTTTTAC
CCAGAAACTA
CAGATATCTA
TGATCGAAAG
AACATGCCAA
GATGTATCTA
CTGTATCCAT
GCACTCAGTT
TGTACCTGTT
CAAGCTAGGC
CTGGCCCCTC
AGATTCAAGA
CCTATATGGA
AAGGTTGACT
TCACAGAAGA
AGAAATCAAC
AACATGAAGA
CTGAGTTGGA
GAAGTATGGC
ATCCAGATGC
CTGCCTTTAG
CAAGATTGGG
GGCATCTTGG
CTAATGAACT
GTCAGTGGAT
GAAGCCGCAT
TACATGCTGC
TGTTATTGCT
ATTAATGAAG
CTATTGACCG
TAGAATTCCA
GCCGACACAT
TTGCAGCTTT
GAAAAATCCG
AATGCCATGC
TTGTAAATCT
TGAAGAGCCC
TTGGCATCCA
CTTACCAGGA
TATACTTTAC
CAGGCTAAGC
AGGACAAAAT
GACAAATGCT
AAAAACAGGA
CAGAAAACTC
AGAGAGAGAA
AGAGATGTTT
ATGAGGAGCT
GCTCACGCAA
GCTGAAATTC
AAGGCAATAT
AAACAAAGTC
AATACATTTT
CTGCATTAGC
AAATATCGAC
CTGGCTTTAG
AACAAGGAGA
TGCACTGGCC
TTGTTCAGGG
CTCTGCAGTC
ACCAGCCCTG
GGGCTTCGAG
GACTGCAGCA
ACAGAATAGC
GACTGGTACT
TGAAGCAGCT
CCTGAGTGAT
AAACAGCAGA
AGAGACAGAG
TGGTCAGACT
GACCCCCTGC
AGAAGGAGGA
GCTGCAGTCT
GGAGTGGATG
CTGCAAACAG
TGCTGCCCAG
CAATATCAGA
GAAGATTGGC
AGCAGTAGCA
CTGATTAATG
CTGCAATCCA
GAAGGGTGTT
GCTGAGAAGA
CTGTTTTGGA
ACTGATGAAT
CCCGAAGCCC
AGCTGCCCCA
GGTGTATCCA
TTTGCCGCCG
ATCTCTATCA
GAAGGAGCTG
GCTACCCTGC
AGCGACAAAG
TCCTGAACAT
AATCTCACCC
ACCCAGAGCT
CTCTGTCGCA
GTGGAGATGT
TGTCATCGGT
GGCCCTGATC
AACAGGGCAT
TGGAATCAGG
AGATGTGAAT
ACAGTGTGGA
AGCAATTGAG
CAGTTCAGTT
ACTGGTCTTA
CCAATATTGA
GGAAGAAAAC
TGTCAGAGGT
ATCTCGATGA
GTTGATGAAA
CTGAAGGCTC
AGGCACATGC
AGAGAATAAT
GAATTCATTA
CATGGAATGA
TATCCAAGCT
TGCGTGGACC
ATGTGAACCT
GGTGGTGCAA
GAGGAACATG
AGAGGATTTT
AGCCATTGGT
TTAATTAATG
AAGCCCTGGA
TGAAGGTGAT
GCCCAAAAGA
CTCTGCAGGC
CCTACAGATT
CCTGCAGCTA
AACTTGAGGG
AGTCCTTGCA
GAAGTGGCCC
AGCATTACCA
AGACACGCTG
ATTAGAGCGA
AGAGAGAGAA
AGCCCAGGAA
ATCCAGGATG
AGTCAGCTGT
GTTATGGTTG
GATGAAATTC
AAGGTGGAAT
CTGGCAGTCC
AACAAAGACA
CCCAAGAAGC
ACAGAAGTTT
GCCTTAGGAA
TCTTTGCCAT
TAATGAGGCA
GTAGAAAGTG
GTGATGTTGG
CAAAACACTG
AGTGCCCTTC
GCTCCCCTGA
TGTTGGCTTG
TATGGAGTCA
TCCCTGAGTG
TGGTGAAACT
TACCACAGTG
ATCTTGCTGA
AGCCAAGAAG
AAAAAACTGG
CAGTAGGAGA
TAATAACAGC
AAGTGGGTGA
AGCACTGGGT
AAAAGGTGGA
TATTATTATT
ACCACAATCT
GGAGACCCAG
GAAGGAGGAT
GGGATGAACC
TCCAAATTTT
GTGCAAAATT
CTATGCAGCT
TTCTCGGGAG
GAGATCCAGA
GTTCTATCTC
TGGGGTGACT
GCCGCATATA
ACCGAGAACA
GCTGTGGCTG
GCCAATGAAG
GCCTGATCAC
CAGGCTGCAG
GCTCGCTGCC
GTGGATACTT
AGTTCGACAG
GAATTCCGAT
CCAGGATGAA
TTTCCTGAAG
AAACAAATCC
CTGCCATCAC
CTGCATTCAG
TCACAGTGGA
GAGGATACAA
GCAGAAGAAG
GCATATCAAG
ATCGGTTAGC
TTACCTGCGC
TCCCACAAAG
ATGAAGTTGT
AAAGATTCAG
TCCCTGGCAA
GGATGCACCA
AGCTCGAAAG
CGCTATCGAG
ATCGCCTGCA
GTACTTCCGG
GACCATATAA
ATGACATTAT
CAAAATCCAG
GCTTTTATTC
GGGCAAACAA
AGCTCGGGAT
GACTACAAGA
CTCTCATCAA
TGCTGAGGAT
CCTCCTATGG
TTGTGGTCCG
AAAATTTGTC
CACCTGCTGG
ACCAAAGTGA
CCAGGATTTT
CAGGAGGAGC
TTGACCTTAT
GAAGATGCGG
GAAGAGGTTA
TCACCCTCAT
TCGTTCTAAC
CAGCAGCTGG
AGAATGACCT
CAATCTCATG
GATATCAAAA
TTGGACTGCT
AGTGAAAAAT
AAGATTACGT
TGCAGGATGT
GGTTTCCCAC
AGTAAAAAAC
TTACCAAAAA
AAATAAGGAA
CAGTTGTCTG
ATATGATGAT
GATAAATAAA
CAGAAGGGAG
GTCTCAAGGC
TTTGAGCAAG
GAGAAGAGAG
AGAAGTTGGA
AGCTTACCAG
CACCTGTTTT
ATTTATTGCA
AACCAATCCC
ACCTATCTGG
CCAAGCTCAT
TTTTCAGATG
CCCCAGAACA
AGTCCACCAA
GTTCATGGAC
TCTGTAATCT
TCACACTCTA
CAACTACGCG
TCCAACCAGC
GAGAGGAGTA
CCTGCTCCTG
CGGCTCTTTA
AGACAGCACT
CCAAGAGGAA
ATCAAGTCGA
AGGTAGATCA
GATTCAAGAG
ATTGTGACAG
GAAATCCTAC
GGTTATTAAA
ATGGTTGTAA
GTTTCAACCG
TGGTGCCCGT
GGCCAGAATG
CCCTGAGACA
GATCTTGGCC
CCAGTCGTGA
AGGAAATTAT
GGATGACAAA
TCTCTCAACA
TCAAAACTGA
CCCTGTGGAT
ATTTACAAAT
CTTGGGTTAA
TCAGATGGAG
TCTCAGACAG
GAGAGGCAAG
CAAACTGCCC
TATGATGTGA
CCCCTGAGCA
GGCGCTAGCT
CATGAAGAAG
TGAAGACACG
GCTAGACAGC
TCCATCAGGA
ACATGCGGGC
TGTGACAGAC
AAGTTTCTCT
CAGCCATTGT
CAGCTCTGTG
GACAAAATCC
CTTATGGGAT
GCGCTTCATT
GCCAAAGTGC
TGAAGGACTC
GTTGCATGAG
AAGTTCCCTG
ATGCTGGTGA
GGATGAGCTG
CTGAAGATTA
TTGGTAACTT
GCTTTATTAT
CGATACATGA
ATCCAGCCAT
TGTTGCTCCT
GATGCCTTTG
ACATCATTGA
CCTGTCAGCA
GGAGGCCAGC
TTACCACAGA
CCAACGCCGA
AATCTGGGCT
CCATTGCAAA
AATGCTTCAG
CATGCTGCTT
CCAATAAGAT
GTTTCTGGGA
GATAATGCCC
ACTTAAGCAT
CATTAATGAA
TATCTTTCCC
AGTCCTACCA
GAAATTCAGA
CGGTTTTTCC
AAACTGCTTG
TGATGTCCCA
GAGCTTCAGG
ATAAATTTAA
TGTGGATGAG
TACTCTGATT
TAGTAACCCT
CACCAAACCA
GTAATCTACA
TTTCCATTGG
TGAAATCATC
AACACCCACA
CTCTCCTGTT
GGATCACCAG
GATGCCATTG
CTCCGGAGCA
CAATGATCCA
ATCCACGAAC
TGCTGGACGA
CCTCGGCGAG
GTGCCCACCA
TCGAGTCCCT
GATAGGGGAA
AGCTCTGGCA
ATTTAAATGA
CCCAAATAAG
GAGGCACTGG
CTAAGACGGA
AGTGTCTCTC
ACCCTGACCA
ACAAGTTCGA
CGTGCCTGGA
GATGAGAATG
CAGAAATGGA
TGCTCGAACC
ATCTTACTGA
ATACAAAACG
TTTAATTGTG
GATGTCATCC
GGTTCCAGCC
AGGAGAGACC
TTGACTGAAA
TCCTAGAAAC
ACCAGCCACC
AGTGAACAGG
AAGCAGAACA
TCAGAGAGCC
ATGCAGAGAC
GTGCTATCCG
TGATGCCAAA
ACACCTGACA
AGATGAAAAA
GTCAAAATCT
GTAAAGGAAG
ACAGCAACCT
CACTCTTCAA
GAGAAGAAAG
AGAAGATCCA
GACAGGTTTA
AAGAAGCTAA
CAGAGCTTGG
AACCGTGGAC
CCAAAGAACA
AATACCAGGA
ACTGATCAAC
GACATTGCCA
GGGATATTCG
GAATCAGCGG
AGGTACCGAC
AGAGGAGAAA
GGCCGAACTA
GTGAAACTGC
AACAGACATA
CGCTGCTCTG
AACTCTAAGG
CCACCTTTTA
TGGGGAGCAG
GTGGATTACT
ATAAAAGCTA
TATCAAAACC
TGCTTGGATA
ACTTAGCCAG
CAAGGGCAAA
GTCTCCAAAA
AGCCTAGGGA
AATGAAAGGA
AAGAAAAGCA
AAAAGATTTC
TCTGAAATAT
ACAGCAGCAA
GACTACATGA
AAAAGGAGTT
CTTCTGGAAA
TTGAGGACCT
GCAAGTGAAT
CAGTTTAAAA
ATGTTATATT
TGAAATCAGT
CCAACAGAAG
AAGTTGGAGA
CTTCGAAGTG
AAAGCCAAAT
TCATGGGAGT
TCAAATGGAG
ACTTTTATGT
TACATTATCA
GGACCTGCTG
CAGCTACAGT
ATGAAGGAGT
TGCAGTCATG
AAATTATTTG
ATAGAGCTAA
AGTAAATGTC
AACCTCCTGA
TCTTCCTTCT
CAACAAAAAG
TTCTACGGGA
AGGGATCCGA
ACAAAAACTA
ATATCAGAGG
AAGATTTGGA
ACAGAAACTA
ATCAGTGAAG
AAGATTTAGA
GCAAAAGTTA
ATTTCCGAGG
AGGACTTATA
GCGGCCGCGA
AGGATCTGCG
ATCGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACGGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGCTGAAGC
TTCGAGGGGC
TCGCATCTCT
CCTTCACGCG
CCCGCCGCCC
TACCTGAGGC
CGCCATCCAC
GCCGGTTGAG
TCGCGTTCTG
CCGCCTCCCG
CCTGTGGTGC
CTCCTGAACT
GCGTCCGCCG
TCTAGGTAAG
TTTAAAGCTC
AGGTCGAGAC
CGGGCCTTTG
TCCGGCGCTC
CCTTGGAGCC
TACCTAGACT
CAGCCGGCTC
TCCACGCTTT
GCCTGACCCT
GCTTGCTCAA
CTCTACGTCT
TTGTTTCGTT
TTCTGTTCTG
CGCCGTTACA
GATCCAAGCT
GTGACCGGCG
CCTACGCTAG
GCCACCATGG
CCAAGCCTTT
GTCTCAAGAA
GAATCCACCC
TCATTGAAAG
AGCAACGGCT
ACAATCAACA
GCATCCCCAT
CTCTGAAGAC
TACAGCGTCG
CCAGCGCAGC
TCTCTCTAGC
GACGGCCGCA
TCTTCACTGG
TGTCAATGTA
TATCATTTTA
CTGGGGGACC
TTGTGCAGAA
CTCGTGGTGC
TGGGCACTGC
TGCTGCTGCG
GCAGCTGGCA
ACCTGACTTG
TATCGTCGCG
ATCGGAAATG
AGAACAGGGG
CATCTTGAGC
CCCTGCGGAC
GGTGCCGACA
GGTGCTTCTC
GATCTGCATC
CTGGGATCAA
AGCCATAGTG
AAGGACAGTG
ATGGACAGCC
GACGGCAGTT
GGGATTCGTG
AATTGCTGCC
CTCTGGTTAT
GTGTGGGAGG
GCTAAGTCGA
CAATCAACCT
CTGGATTACA
AAATTTGTGA
AAGATTGACT
GGTATTCTTA
ACTATGTTGC
TCCTTTTACG
CTATGTGGAT
ACGCTGCTTT
AATGCCTTTG
TATCATGCTA
TTGCTTCCCG
TATGGCTTTC
ATTTTCTCCT
CCTTGTATAA
ATCCTGGTTG
CTGTCTCTTT
ATGAGGAGTT
GTGGCCCGTT
GTCAGGCAAC
GTGGCGTGGT
GTGCACTGTG
TTTGCTGACG
CAACCCCCAC
TGGTTGGGGC
ATTGCCACCA
CCTGTCAGCT
CCTTTCCGGG
ACTTTCGCTT
TCCCCCTCCC
TATTGCCACG
GCGGAACTCA
TCGCCGCCTG
CCTTGCCCGC
TGCTGGACAG
GGGCTCGGCT
GTTGGGCACT
GACAATTCCG
TGGTGTTGTC
GGGGAAATCA
TCGTCCTTTC
CTTGGCTGCT
CGCCTGTGTT
GCCACCTGGA
TTCTGCGCGG
GACGTCCTTC
TGCTACGTCC
CTTCGGCCCT
CAATCCAGCG
GACCTTCCTT
CCCGCGGCCT
GCTGCCGGCT
CTGCGGCCTC
TTCCGCGTCT
TCGCCTTCGC
CCTCAGACGA
GTCGGATCTC
CCTTTGGGCC
GCCTCCCCGC
CTGGTACCTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
AATAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTA
GTAGTTCATG
TCATCTTATT
ATTCAGTATT
TATAACTTGC
AAAGAAATGA
ATATCAGAGA
GTGAGAGGAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGG
CTCTAGCTAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGACT
TTTGCAGAGA
CGGCCCAAAT
TCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCCCTTTC
GTCTCGCGCG
TTTCGGTGAT
GACGGTGAAA
ACCTCTGACA
CATGCAGCTC
CCGGAGACGG
TCACAGCTTG
TCTGTAAGCG
GATGCCGGGA
GCAGACAAGC
CCGTCAGGGC
GCGTCAGCGG
GTGTTGGCGG
GTGTCGGGGC
TGGCTTAACT
ATGCGGCATC
AGAGCAGATT
GTACTGAGAG
TGCACCATAT
GCGGTGTGAA
ATACCGCACA
GATGCGTAAG
GAGAAAATAC
CGCATCAGGC
GCCATTCGCC
ATTCAGGCTG
CGCAACTGTT
GGGAAGGGCG
ATCGGTGCGG
GCCTCTTCGC
TATTACGCCA
GCTGGCGAAA
GGGGGATGTG
CTGCAAGGCG
ATTAAGTTGG
GTAACGCCAG
GGTTTTCCCA
GTCACGACGT
TGTAAAACGA
CGGCCAGTGC
CAAGCTG
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