产品货号：P72169
产品规格：
产品数量：
产品价格：￥0.00
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| 名称: | pLV3-CMV-DDX60(human)-3×FLAG-EF1a-mCherry-Blast（1同义突变） |
|---|---|
| 别称: | NM_017631.6;DDX60 |
| 基因长: | 5139bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Blast |
| 荧光标记： | 红色 |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLV3-CMV-MCS-3×FLAG-EF1a-mCherry-Blast |
| 正向引物: | EF1a-R |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-DDX60(human)-3×FLAG-EF1a-mCherry-Blast是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动人源DDX60基因转录，促进Anxa11基因C端融合表达3×FLAG标签，便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。此外，质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装，便于基因稳定整合至宿主细胞。质粒携带的mCherry红色荧光蛋白用于观察目的基因的表达和定位情况。质粒携带Blast抗性基因，用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
GAATTCGCCA
CCATGGAAAG
AAATGTTCTT
ACAACATTTT
CACAGGAAAT
GTCCCAGTTA
ATTTTGAATG
AAATGCCAAA
AGCTGAATAT
TCCAGTTTAT
TCAATGATTT
TGTTGAATCT
GAATTTTTTT
TGATTGATGG
GGATTCATTA
CTTATCACAT
GTATCTGTGA
GATATCATTT
AAGCCTGGGC
AGAACCTCCA
TTTCTTCTAT
CTGGTTGAAC
GCTATCTTGT
GGATCTTATT
AGCAAAGGAG
GACAATTCAC
CATAGTTTTC
TTCAAGGATG
CCGAGTATGC
GTATTTCAAC
TTCCCTGAAC
TTCTTTCTTT
GAGAACTGCT
TTAATTCTTC
ATCTTCAGAA
GAATACCACG
ATCGACGTGA
GGACGACATT
TTCGAGATGC
TTATCAAAAG
AGTGGGGAAG
TTTCTTGGAA
GAGAGTTACC
CATATTTCCT
GATAGTTGCA
GACGAAGGCC
TGAACGATCT
ACAAACACAG
CTTTTCAACT
TTTTAATCAT
TCATTCTTGG
GCAAGGAAGG
TCAACGTTGT
ACTTTCCTCA
GGGCAAGAAT
CTGATGTTCT
TTGCCTTTAT
GCATACCTTC
TTCCAAGCAT
GTACAGACAC
CAGATTTTTT
CCTGGAAGAA
TAAGCAGAAC
ATTAAAGATG
CTTATACAAC
CCTGCTTAAC
CAGTTGGAAA
GATTTAAGCT
TTCAGCATTA
GCACCTCTTT
TTGGAAGTTT
AAAATGGAAT
AATATTACGG
AAGAGGCACA
CAAGACTGTA
TCTCTGCTTA
CACAAGTCTG
GCCAGAAGGA
TCTGACATTC
GGCGTGTCTT
TTGTGTTACT
TCATGCTCAT
TATCTTTGAG
AATGTACCAT
CGCTTTTTAG
GAAACAGAGA
GCCCTCCTCT
GGTCAGGAAA
CTGAGATCCA
ACAGGTGAAC
AGTAATTGCT
TAACCCTGCA
GGAGATGGAA
GATTTGTGTA
AACTGCATTG
TCTCACTGTG
GTTTTTCTAC
TCCATCTGCC
TCTTTCTCAA
AGAGCTTGTG
CTAGAGTCAT
CACTTCCCAT
TGGGCTGAGG
ACATGAAGCC
TTTATTACAA
ATGAAAAAGT
GGTGTGAATA
TTTCATCTTA
AGAAATATAC
ATACTTTTGA
ATTTTGGAAT
CTGAATTTAA
TTCACCTTTC
TGACTTAAAT
GATGAGCTTT
TGTTGAAGAA
TATTGCTTTT
TACTATGAAA
ATGAAAATGT
AAAAGGCCTA
CATTTGAATT
TGGGAGATAC
CATTATGAAA
GATTATGAAT
ATCTCTGGAA
TACCGTATCA
AAGTTGGTCA
GAGACTTTGA
GGTTGGACAG
CCATTTCCTC
TGAGAACAAC
AAAAGTTTGT
TTTCTTGAAA
AGAAACCATC
ACCAATCAAA
GACAGCTCCA
ATGAAATGGT
GCCCAATTTG
GGTTTTATTC
CAACGTCATC
TTTTGTGGTT
GATAAATTTG
CTGGAGATAT
TTTGAAAGAT
TTGCCTTTTC
TAAAGAGTGA
TGATCCTATT
GTTACTTCAC
TGGTTAAACA
AAAGGAATTT
GATGAACTTG
TGCACTGGCA
TTCTCATAAA
CCCCTGAGTG
ATGATTATGA
CAGGTCCAGG
TGTCAGTTTG
ATGAAAAATC
TAGAGACCCT
CGTGTTCTTA
GATCTGTGCA
AAAGTATCAT
GTTTTCCAAC
GGTTTTATGG
GAATTCATTA
GAAACAGTCT
CTTCGAAAAT
CATCGTGACT
CAAACTATTA
AGTCAAAGAA
GGATTTTAGT
GGGCCCAAGA
GCAAAAAGGC
ACACGAGACC
AAGGCTGAAA
TAATTGCTAG
AGAGAATAAG
AAAAGGTTAT
TTGCCAGGGA
AGAACAAAAG
GAAGAGCAAA
AGTGGAATGC
TTTGTCATTT
TCTATTGAAG
AGCAATTGAA
AGAAAATTTA
CACTCTGGAA
TAAAGAGCCT
GGAAGATTTT
TTGAAATCCT
GTAAAAGTAG
CTGTGTGAAA
CTTCAGGTTG
AAATGGTGGG
GTTAACTGCT
TGCTTGAAAG
CCTGGAAAGA
ACATTGCCGA
AGTGAAGAAG
GTAAAACCAC
GAAAGATTTA
AGTATAGCTG
TTCAGGTGAT
GAAAAGGATC
CACTCCTTGA
TGGAAAAATA
CTCAGAACTT
TTACAAGAAG
ATGATCGGCA
ACTCATAGCC
AGATGCCTTA
AGTATTTAGG
ATTTGATGAG
TTGGCAAGTT
CTTTACATCC
AGCCCAGGAT
GCAGAAAATG
ATGTAAAAGT
GAAGAAAAGG
AATAAATATT
CAGTTGGCAT
TGGGCCAGCT
CGGTTCCAAC
TGCAATACAT
GGGCCATTAT
TTGATACGAG
ATGAGAGAAA
AGACCCAGAT
CCCAGGGTCC
AGGATTTTAT
TCCCGACACA
TGGCAGCGAG
AGCTCCTTGA
TGTTGTGGAT
AAGAATGAGT
CAGCAGTGAT
TGTTGCCCCA
ACGTCCTCAG
GCAAAACCTA
TGCCTCCTAC
TACTGTATGG
AGAAAGTGCT
GAAGGAGAGC
GACGACGGGG
TGGTCGTGTA
CGTTGCACCC
ACAAAGGCCC
TTGTTAATCA
AGTGGCAGCA
ACTGTTCAGA
ATCGTTTTAC
GAAAAATCTG
CCAAGTGGTG
AAGTTCTCTG
TGGTGTTTTC
ACCAGGGAGT
ATCGTCATGA
TGCCTTAAAC
TGTCAGGTAC
TTATTACAGT
GCCTGCCTGC
TTTGAAATTC
TGCTGCTTGC
TCCTCATCGC
CAAAACTGGG
TGAAAAAGAT
CAGATATGTT
ATATTTGATG
AGGTTCATTG
TCTTGGTGGA
GAAATTGGAG
CAGAAATCTG
GGAACATCTC
CTTGTCATGA
TCCGATGTCC
CTTTTTGGCT
CTTTCAGCTA
CCATAAGTAA
TCCTGAACAT
CTCACCGAGT
GGCTACAATC
GGTAAAATGG
TACTGGAAAC
AAGAAGACAA
AATAATTGAA
AATAATACCG
CTTCTAAAAG
ACATGTGGGT
CGTCAGGCCG
GCTTTCCCAA
AGACTACTTG
CAAGTAAAAC
AATCGTATAA
AGTTAGACTT
GTGCTCTATG
GAGAGAGGTA
TAATGATCTA
GAGAAGCATG
TATGTTCAAT
AAAACATGGT
GACATTCATT
TTGATCATTT
TCACCCATGT
GCTGCACTAA
CAACAGATCA
TATTGAAAGG
TATGGATTCC
CTCCTGATCT
TACCCTTTCA
CCTCGAGAAA
GCATCCAGCT
GTATGATGCC
ATGTTTCAAA
TTTGGAAAAG
TTGGCCTCGG
GCCCAGGAAC
TGTGCCCAGA
AAACTTCATT
CATTTTAACA
ATAAATTAGT
CATTAAAAAG
ATGGATGCTA
GGAAATATGA
AGAGAGTCTA
AAGGCAGAAT
TAACAAGTTG
GATTAAAAAT
GGCAACGTAG
AGCAGGCCAG
AATGGTACTT
CAGAATCTTA
GTCCTGAAGC
AGATTTGAGT
CCAGAAAACA
TGATCACCAT
GTTTCCACTT
CTAGTTGAAA
AACTAAGGAA
AATGGAGAAG
TTACCTGCAC
TATTTTTTTT
ATTCAAGTTA
GGAGCTGTAG
AAAACGCAGC
TGAAAGTGTG
AGCACTTTCC
TAAAGAAAAA
GCAGGAGACA
AAAAGGCCTC
CCAAAGCTGA
TAAAGAAGCC
CATGTCATGG
CTAACAAACT
TCGAAAAGTT
AAAAAATCCA
TAGAGAAACA
AAAGATCATA
GATGAAAAGA
GCCAGAAAAA
AACCAGAAAT
GTGGATCAAA
GCCTAATACA
TGAAGCTGAA
CATGATAATC
TAGTGAAGTG
TCTAGAGAAG
AACCTGGAAA
TCCCACAGGA
CTGCACATAT
GCTGATCAAA
AAGCAGTGGA
CACTGAGACT
TTGCAGAAGG
TATTTGGTCG
AGTAAAATTT
GAAAGAAAAG
GTGAAGAATT
GAAAGCCTTG
GCAGAAAGGG
GTATTGGATA
TCATCACAGT
GCTATGAGTT
TCAAAGAAAA
ACAATTAGTT
GAAATCCTCT
TTAGAAAAGG
ATATCTTAGG
GTGGTGACAG
CTACTGGAAC
ACTTGCTTTA
GGTGTCAACA
TGCCTTGTAA
ATCTGTGGTT
TTTGCTCAAA
ACTCAGTCTA
TCTGGATGCG
TTGAATTATA
GACAGATGTC
TGGCCGTGCT
GGAAGAAGAG
GTCAAGACCT
GATGGGAGAT
GTATATTTCT
TTGATATTCC
ATTCCCCAAA
ATAGGAAAAC
TCATAAAATC
CAATGTTCCT
GAGCTGAGAG
GACACTTCCC
TCTCAGCATA
ACCCTGGTCC
TGCGACTCAT
GCTGCTGGCT
TCCAAGGGAG
ATGACCCAGA
GGATGCCAAG
GCAAAGGTGC
TATCAGTGCT
AAAGCATTCA
TTGCTGTCCT
TCAAGCAACC
CAGAGTCATG
GACATGTTAA
AACTTTACTT
CCTGTTTTCT
TTGCAGTTCC
TGGTGAAAGA
GGGCTATTTA
GATCAAGAAG
GTAATCCTAT
GGGGTTTGCT
GGACTTGTAT
CACATTTGCA
TTATCATGAA
CCTTCTAATC
TTGTTTTTGT
CAGTTTTCTT
GTAAATGGAC
TCTTCCATGA
TCTCTGTCAG
CCAACCAGGA
AAGGCTCAAA
ACATTTTTCT
CAAGACGTTA
TGGAAAAGCT
AGTATTAGTA
TTGGCACATC
TCTTTGGAAG
AAGATATTTT
CCACCAAAGT
TCCAAGATGC
ACACTTCGAG
TTTTATCAAT
CAAAGGTGTT
CCTTGATGAT
CTCCCTGAGG
ATTTTAGTGA
TGCTTTAGAT
GAATATAACA
TGAAAATTAT
GGAGGACTTT
ACCACTTTCC
TACGAATTGT
TTCCAAACTG
GCTGATATGA
ATCAGGAATA
TCAACTCCCA
TTGTCAAAAA
TCAAATTCAC
AGGTAAAGAA
TGTGAAGACT
CTCAACTCGT
ATCTCATTTG
ATGAGCTGCA
AGGAAGGAAG
AGTAGCAATT
TCACCATTTG
TTTGTCTGTC
TGGGAACTTT
GATGATGATT
TGCTTCGACT
AGAAACTCCA
AACCATGTTA
CTCTAGGCAC
AATCGGTGTC
AATCGCTCTC
AGGCTCCAGT
GCTGTTGTCA
CAGAAATTTG
ATAACCGAGG
AAGGAAAATG
TCGCTTAATG
CCTATGCACT
GGATTTCTAC
AAACATGGTT
CCTTGATAGG
ATTAGTCCAG
GATAACAGGA
TGAATGAAGG
AGATGCTTAT
TATTTGTTGA
AGGATTTTGC
ACTCACCATT
AAATCTATCA
GTGTTTCCTT
GCGTGAGCTA
TGTGAAAATG
AAGACGACAA
CGTTGTCTTA
GCCTTTGAAC
AACTGAGTAC
AACTTTTTGG
GAAAAGTTAA
ACAAAGTCGG
ATCCGACTAC
AAAGACCATG
ACGGTGATTA
TAAAGATCAT
GACATCGACT
ACAAGGATGA
CGATGACAAG
TAGCGGCCGC
GAAGGATCTG
CGATCGCTCC
GGTGCCCGTC
AGTGGGCAGA
GCGCACATCG
CCCACAGTCC
CCGAGAAGTT
GGGGGGAGGG
GTCGGCAATT
GAACGGGTGC
CTAGAGAAGG
TGGCGCGGGG
TAAACTGGGA
AAGTGATGTC
GTGTACTGGC
TCCGCCTTTT
TCCCGAGGGT
GGGGGAGAAC
CGTATATAAG
TGCAGTAGTC
GCCGTGAACG
TTCTTTTTCG
CAACGGGTTT
GCCGCCAGAA
CACAGCTGAA
GCTTCGAGGG
GCTCGCATCT
CTCCTTCACG
CGCCCGCCGC
CCTACCTGAG
GCCGCCATCC
ACGCCGGTTG
AGTCGCGTTC
TGCCGCCTCC
CGCCTGTGGT
GCCTCCTGAA
CTGCGTCCGC
CGTCTAGGTA
AGTTTAAAGC
TCAGGTCGAG
ACCGGGCCTT
TGTCCGGCGC
TCCCTTGGAG
CCTACCTAGA
CTCAGCCGGC
TCTCCACGCT
TTGCCTGACC
CTGCTTGCTC
AACTCTACGT
CTTTGTTTCG
TTTTCTGTTC
TGCGCCGTTA
CAGATCCAAG
CTGTGACCGG
CGCCTACGCT
AGACGCCACC
ATGGTGAGCA
AGGGCGAGGA
GGATAACATG
GCCATCATCA
AGGAGTTCAT
GCGCTTCAAG
GTGCACATGG
AGGGCTCCGT
GAACGGCCAC
GAGTTCGAGA
TCGAGGGCGA
GGGCGAGGGC
CGCCCCTACG
AGGGCACCCA
GACCGCCAAG
CTGAAGGTGA
CCAAGGGTGG
CCCCCTGCCC
TTCGCCTGGG
ACATCCTGTC
CCCTCAGTTC
ATGTACGGCT
CCAAGGCCTA
CGTGAAGCAC
CCCGCCGACA
TCCCCGACTA
CTTGAAGCTG
TCCTTCCCCG
AGGGCTTCAA
GTGGGAGCGC
GTGATGAACT
TCGAGGACGG
CGGCGTGGTG
ACCGTGACCC
AGGACTCCTC
CCTGCAGGAC
GGCGAGTTCA
TCTACAAGGT
GAAGCTGCGC
GGCACCAACT
TCCCCTCCGA
CGGCCCCGTA
ATGCAGAAGA
AGACCATGGG
CTGGGAGGCC
TCCTCCGAGC
GGATGTACCC
CGAGGACGGC
GCCCTGAAGG
GCGAGATCAA
GCAGAGGCTG
AAGCTGAAGG
ACGGCGGCCA
CTACGACGCT
GAGGTCAAGA
CCACCTACAA
GGCCAAGAAG
CCCGTGCAGC
TGCCCGGCGC
CTACAACGTC
AACATCAAGT
TGGACATCAC
CTCCCACAAC
GAGGACTACA
CCATCGTGGA
ACAGTACGAA
CGCGCCGAGG
GCCGCCACTC
CACCGGCGGC
ATGGACGAGC
TGTACAAGGG
ATCTCGCGAG
GGCAGAGGAA
GTCTTCTAAC
ATGCGGTGAC
GTGGAGGAGA
ATCCCGGCCC
TATGGCCAAG
CCTTTGTCTC
AAGAAGAATC
CACCCTCATT
GAAAGAGCAA
CGGCTACAAT
CAACAGCATC
CCCATCTCTG
AAGACTACAG
CGTCGCCAGC
GCAGCTCTCT
CTAGCGACGG
CCGCATCTTC
ACTGGTGTCA
ATGTATATCA
TTTTACTGGG
GGACCTTGTG
CAGAACTCGT
GGTGCTGGGC
ACTGCTGCTG
CTGCGGCAGC
TGGCAACCTG
ACTTGTATCG
TCGCGATCGG
AAATGAGAAC
AGGGGCATCT
TGAGCCCCTG
CGGACGGTGC
CGACAGGTGC
TTCTCGATCT
GCATCCTGGG
ATCAAAGCCA
TAGTGAAGGA
CAGTGATGGA
CAGCCGACGG
CAGTTGGGAT
TCGTGAATTG
CTGCCCTCTG
GTTATGTGTG
GGAGGGCTAA
GTCGACAATC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AGCTGACGTC
CTTTCCATGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTCCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGCCGCCTCC
CCGCCTGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGATAAG
ATCTGCTTTT
TGCTTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTAGTAGTT
CATGTCATCT
TATTATTCAG
TATTTATAAC
TTGCAAAGAA
ATGAATATCA
GAGAGTGAGA
GGAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGGCTCTAG
CTATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CGGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GACTTTTGCA
GAGACGGCCC
AAATTCGTAA
TCATGGTCAT
AGCTGTTTCC
TGTGTGAAAT
TGTTATCCGC
TCACAATTCC
ACACAACATA
CGAGCCGGAA
GCATAAAGTG
TAAAGCCTGG
GGTGCCTAAT
GAGTGAGCTA
ACTCACATTA
ATTGCGTTGC
GCTCACTGCC
CGCTTTCCAG
TCGGGAAACC
TGTCGTGCCA
GCTGCATTAA
TGAATCGGCC
AACGCGCGGG
GAGAGGCGGT
TTGCGTATTG
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGGA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
CAATGCTTAA
TCAGTGAGGC
ACCTATCTCA
GCGATCTGTC
TATTTCGTTC
ATCCATAGTT
GCCTGACTCC
CCGTCGTGTA
GATAACTACG
ATACGGGAGG
GCTTACCATC
TGGCCCCAGT
GCTGCAATGA
TACCGCGAGA
CCCACGCTCA
CCGGCTCCAG
ATTTATCAGC
AATAAACCAG
CCAGCCGGAA
GGGCCGAGCG
CAGAAGTGGT
CCTGCAACTT
TATCCGCCTC
CATCCAGTCT
ATTAATTGTT
GCCGGGAAGC
TAGAGTAAGT
AGTTCGCCAG
TTAATAGTTT
GCGCAACGTT
GTTGCCATTG
CTACAGGCAT
CGTGGTGTCA
CGCTCGTCGT
TTGGTATGGC
TTCATTCAGC
TCCGGTTCCC
AACGATCAAG
GCGAGTTACA
TGATCCCCCA
TGTTGTGCAA
AAAAGCGGTT
AGCTCCTTCG
GTCCTCCGAT
CGTTGTCAGA
AGTAAGTTGG
CCGCAGTGTT
ATCACTCATG
GTTATGGCAG
CACTGCATAA
TTCTCTTACT
GTCATGCCAT
CCGTAAGATG
CTTTTCTGTG
ACTGGTGAGT
ACTCAACCAA
GTCATTCTGA
GAATAGTGTA
TGCGGCGACC
GAGTTGCTCT
TGCCCGGCGT
CAATACGGGA
TAATACCGCG
CCACATAGCA
GAACTTTAAA
AGTGCTCATC
ATTGGAAAAC
GTTCTTCGGG
GCGAAAACTC
TCAAGGATCT
TACCGCTGTT
GAGATCCAGT
TCGATGTAAC
CCACTCGTGC
ACCCAACTGA
TCTTCAGCAT
CTTTTACTTT
CACCAGCGTT
TCTGGGTGAG
CAAAAACAGG
AAGGCAAAAT
GCCGCAAAAA
AGGGAATAAG
GGCGACACGG
AAATGTTGAA
TACTCATACT
CTTCCTTTTT
CAATATTATT
GAAGCATTTA
TCAGGGTTAT
TGTCTCATGA
GCGGATACAT
ATTTGAATGT
ATTTAGAAAA
ATAAACAAAT
AGGGGTTCCG
CGCACATTTC
CCCGAAAAGT
GCCACCTGAC
GTCTAAGAAA
CCATTATTAT
CATGACATTA
ACCTATAAAA
ATAGGCGTAT
CACGAGGCCC
TTTCGTCTCG
CGCGTTTCGG
TGATGACGGT
GAAAACCTCT
GACACATGCA
GCTCCCGGAG
ACGGTCACAG
CTTGTCTGTA
AGCGGATGCC
GGGAGCAGAC
AAGCCCGTCA
GGGCGCGTCA
GCGGGTGTTG
GCGGGTGTCG
GGGCTGGCTT
AACTATGCGG
CATCAGAGCA
GATTGTACTG
AGAGTGCACC
ATATGCGGTG
TGAAATACCG
CACAGATGCG
TAAGGAGAAA
ATACCGCATC
AGGCGCCATT
CGCCATTCAG
GCTGCGCAAC
TGTTGGGAAG
GGCGATCGGT
GCGGGCCTCT
TCGCTATTAC
GCCAGCTGGC
GAAAGGGGGA
TGTGCTGCAA
GGCGATTAAG
TTGGGTAACG
CCAGGGTTTT
CCCAGTCACG
ACGTTGTAAA
ACGACGGCCA
GTGCCAAGCT
G
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