名称: | pCMV-HIVEP3(human)-3×FLAG-Neo |
---|---|
别称: | NM_024503.5;KBP-1; KBP1; KRC; Schnurri-3; SHN3; ZAS3;ZNF40C; |
基因长: | 7218bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-T7-MCS-3×FLAG-WPRE-Neo |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pCMV-HIVEP3(human)-3×FLAG-Neo是适用于哺乳细胞过表达的质粒。质粒带有的CMV增强子和CMV启动子可联合启动人源HIVEP3基因高效表达,促进HIVEP3基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。WPRE蛋白可增强目的基因转录后的加工和稳定性,有助于增加mRNA的半衰期和翻译效率。质粒携带Neo/G418抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTAGGCGTT
TTGCGCTGCT
TCGCGATGTA
CGGGCCAGAT
ATACGCGTTG
ACATTGATTA
TTGACTAGTT
ATTAATAGTA
ATCAATTACG
GGGTCATTAG
TTCATAGCCC
ATATATGGAG
TTCCGCGTTA
CATAACTTAC
GGTAAATGGC
CCGCCTGGCT
GACCGCCCAA
CGACCCCCGC
CCATTGACGT
CAATAATGAC
GTATGTTCCC
ATAGTAACGC
CAATAGGGAC
TTTCCATTGA
CGTCAATGGG
TGGAGTATTT
ACGGTAAACT
GCCCACTTGG
CAGTACATCA
AGTGTATCAT
ATGCCAAGTA
CGCCCCCTAT
TGACGTCAAT
GACGGTAAAT
GGCCCGCCTG
GCATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTCTA
TATAAGCAGA
GCTCTCTGGC
TAACTAGAGA
ACCCACTGCT
TACTGGCTTA
TCGAAATTAA
TACGACTCAC
TATAGGGAGA
CCCAAGCTGG
CTAGCGTTTA
AACTTAAGCT
TGGTACCGAG
CTCGAATTCG
CCACCATGGA
TCCTGAACAA
AGTGTCAAGG
GCACCAAGAA
GGCTGAGGGA
AGTCCCCGGA
AGCGGCTGAC
CAAAGGAGAG
GCCATTCAGA
CCAGTGTTTC
TTCCAGCGTC
CCATACCCAG
GCAGCGGCAC
AGCTGCCACC
CAAGAGAGCC
CCGCCCAAGA
GCTCTTAGCC
CCGCAGCCCT
TCCCGGGCCC
CTCATCAGTT
CTTAGGGAAG
GCTCTCAGGA
GAAAACGGGC
CAGCAGCAGA
AGCCCCCCAA
AAGGCCCCCC
ATCGAAGCAT
CCGTCCACAT
CTCACAGCTT
CCGCAGCACC
CTCTGACACC
AGCATTCATG
TCGCCTGGCA
AACCTGAGCA
TCTCCTGGAG
GGGTCCACAT
GGCAACTGGT
TGACCCCATG
AGACCTGGAC
CCTCTGGCTC
CTTCGTGGCC
CCTGGGCTCC
ATCCTCAGAG
CCAGCTCCTT
CCTTCCCACG
CTTCCATCAT
TCCCCCCGAG
GACCTTCCTG
GAGTCCCCAA
AGTCTTCGTG
CCTCGTCCTT
CCCAGGTCTC
CTTGAAGCCC
ACAGAAGAGG
CACACAAGAA
GGAGAGGAAG
CCCCAGAAGC
CAGGCAAGTA
CATCTGCCAG
TACTGCAGCC
GGCCCTGTGC
CAAGCCCAGC
GTGCTCCAGA
AGCACATTCG
CTCACACACA
GGTGAGAGGC
CCTACCCCTG
CGGCCCCTGT
GGCTTCTCCT
TCAAGACCAA
GAGTAATCTC
TACAAGCACA
GGAAGTCCCA
TGCCCACCGC
ATCAAAGCAG
GCCTGGCCTC
AGGCATGGGT
GGCGAGATGT
ACCCACATGG
GCTGGAGATG
GAGCGGATCC
CTGGGGAAGA
GTTTGAGGAG
CCCACTGAGG
GAGAAAGCAC
AGATTCTGAA
GAGGAGACTA
GTGCCACCTC
TGGTCACCCT
GCAGAGCTCT
CCCCAAGACC
CAAGCAGCCC
CTTCTCTCCA
GCGGGCTATA
CAGCTCTGGG
AGCCACAGTT
CCAGCCACGA
ACGCTGTTCC
CTGTCCCAGT
CCAGCACAGC
CCAGTCACTC
GAAGACCCCC
CTCCATTTGT
GGAACCCTCA
TCTGAGCACC
CCCTGAGCCA
TAAACCTGAA
GACACCCACA
CGATTAAGCA
GAAGCTGGCC
CTCCGCTTAA
GCGAGAGGAA
GAAGGTGATC
GATGAGCAGG
CGTTTCTGAG
CCCAGGCAGC
AAAGGGAGTA
CTGAGTCTGG
GTATTTCTCT
CGCTCCGAGA
GTGCAGAGCA
GCAGGTCAGC
CCCCCAAACA
CCAACGCCAA
GTCCTACGCT
GAGATCATCT
TTGGCAAGTG
TGGGCGAATA
GGACAGCGGA
CCGCCATGCT
GACAGCCACC
TCCACCCAGC
CCCTCCTGCC
CCTGTCCACC
GAAGACAAGC
CCAGCCTGGT
GCCTTTGTCT
GTACCCCGGA
CGCAGGTGAT
CGAGCACATC
ACGAAGCTCA
TCACCATCAA
CGAGGCCGTG
GTGGACACCA
GCGAGATCGA
CAGCGTGAAG
CCAAGGCGGA
GCTCACTGTC
CAGGCGCAGC
AGCATGGAGT
CCCCAAAATC
CAGCCTCTAC
CGGGAGCCCC
TGTCATCCCA
CAGTGAGAAA
ACCAAGCCTG
AACAATCACT
GCTGAGCCTC
CAGCACCCGC
CCAGTACCGC
CCCCCCTGTG
CCTCTCCTGA
GAAGCCACTC
AATGCCTTCT
GCCGCCTGCA
CTATCAGCAC
CCCCCACCAC
CCCTTCCGAG
GTAGCTACTC
CTTCGATGAC
CATATCACCG
ACTCCGAAGC
CCTGAGCCAC
AGCAGTCACG
TGTTTACCTC
CCACCCCCGG
ATGCTGAAGC
GCCAGCCGGC
AATCGAATTA
CCTTTGGGAG
GGGAATACAG
TTCTGAGGAG
CCTGGCCCAA
GCAGCAAAGA
CACAGCCTCC
AAGCCCTCGG
ACGAAGTGGA
ACCCAAGGAA
AGCGAGCTTA
CCAAAAAGAC
CAAGAAGGGT
TTGAAAACAA
AAGGGGTGAT
CTACGAATGT
AACATATGTG
GTGCTCGGTA
CAAGAAAAGG
GATAACTACG
AAGCCCACAA
AAAATACTAC
TGCTCAGAGC
TTCAGATCGC
AAAGCCCATC
TCTGCAGGCA
CCCACACATC
TCCAGAAGCT
GAAAAGAGTC
AGATTGAGCA
TGAGCCGTGG
TCCCAAATGA
TGCATTACAA
ACTGGGAACC
ACCCTGGAAC
TCACTCCACT
GAGGAAGAGG
AGGAAAGAGA
AGAGCCTTGG
GGACGAGGAA
GAGCCACCTG
CCTTTGAGTC
CACAAAAAGT
CAGTTTGGCA
GCCCCGGGCC
ATCTGATGCT
GCTCGGAACC
TTCCCCTGGA
GTCCACCAAG
TCACCAGCAG
AACCAAGTAA
ATCAGTGCCC
TCCTTGGAGG
GACCCACGGG
CTTCCAGCCA
AGGACTCCCA
AGCCAGGGTC
CGGTTCAGAA
TCAGGGAAGG
AGAGGAGAAC
AACGTCCAAA
GAAATTTCTG
TCATCCAGCA
CACCAGCTCC
TTTGAGAAAT
CTGATTCTCT
CGAGCAGCCG
AGTGGCTTGG
AAGGGGAAGA
CAAACCTCTG
GCCCAGTTCC
CATCACCCCC
ACCTGCCCCA
CACGGACGCT
CTGCTCACTC
CCTGCAGCCT
AAGTTGGTCC
GCCAGCCCAA
CATTCAGGTT
CCTGAGATCC
TAGTAACTGA
GGAGCCTGAC
CGGCCGGACA
CAGAGCCAGA
GCCGCCCCCT
AAGGAACCTG
AGAAGACTGA
GGAGTTCCAA
TGGCCCCAGC
GCAGCCAGAC
ACTTGCCCAG
CTCCCAGCTG
AGAAGCTGCC
ACCCAAAAAG
AAGAGGTTGC
GCCTGGCAGA
GATGGCCCAA
TCATCAGGGG
AGTCCAGCTT
CGAGTCCTCT
GTGCCTCTGT
CTCGCAGCCC
GAGCCAGGAA
AGCAATGTCT
CTTTGAGTGG
GTCCAGCCGC
TCAGCCTCGT
TTGAGAGGGA
TGACCATGGG
AAAGCCGAGG
CCCCCAGTCC
CTCATCTGAC
ATGCGCCCCA
AACCCCTGGG
CACCCACATG
TTGACTGTCC
CCAGCCACCA
CCCACATGCC
CGAGAGATGC
GGAGGTCAGC
CTCAGAGCAG
AGCCCCAACG
TTTCCCATTC
TGCCCACATG
ACCGAGACAC
GCAGCAAATC
CTTTGACTAT
GGCAGCTTGT
CCTTGACAGG
CCCTTCTGCT
CCAGCCCCAG
TGGCTCCACC
AGCGCGGGTG
GCCCCGCCAG
AGAGAAGAAA
ATGCTTCTTG
GTGAGACAGG
CCTCTCTGAG
CAGGCCTCCA
GAATCTGAGT
TGGAGGTTGC
CCCCAAGGGA
AGACAGGAGA
GCGAAGAACC
ACAGCCCTCA
TCCAGTAAAC
CCTCTGCCAA
AAGCTCATTG
TCCCAGATTT
CCTCTGCGGC
CACCTCACAT
GGTGGACCCC
CGGGAGGCAA
GGGCCCAGGG
CAGGACAGGC
CCCCATTGGG
GCCCACTGTG
CCCTACACAG
AAGCACTGCA
AGTGTTCCAC
CACCCCGTTG
CCCAGACACC
CCTGCATGAG
AAGCCATACC
TGCCCCCACC
AGTCTCCCTT
TTCTCCTTCC
AGCATCTCGT
GCAGCATGAG
CCAGGACAGT
CTCCAGAATT
CTTCTCCACC
CAGGCCATGT
CCAGCCTCCT
GTCCTCACCA
TACTCCATGC
CCCCACTTCC
TCCCTCCTTA
TTTCAAGCCC
CACCGCTTCC
TCTCCAGCCT
ACTGTTCTGC
ACCCAGGCCA
ACTCCATCTC
CCCCAGCTCA
TGCCTCACCC
AGCCAACATC
CCCTTCAGGC
AGCCCCCTTC
CTTCCTCCCC
ATGCCATACC
CGACCTCCTC
AGCACTGTCT
TCTGGGTTTT
TCCTGCCTCT
GCAATCCCAG
TTTGCACTTC
AGCTCCCTGG
TGATGTGGAA
AGCCATCTGC
CCCAGATCAA
AACCAGCCTG
GCCCCACTGG
CAACAGGAAG
TGCTGGCCTC
TCCCCCAGCA
CAGAGTACAG
CAGTGACATC
CGGCTACCCC
CTGTGGCTCC
CCCAGCCAGC
TCCTCAGCAC
CTACATCAGC
TCCTCCACTG
GCCCTGCCTG
CCTGTCCAGA
CACCATGGTG
TCCCTGGTTG
TGCCTGTCCG
TGTTCAGACC
AATATGCCGT
CCTATGGGAG
CGCAATGTAC
ACCACCCTTT
CCCAGATCTT
GGTCACCCAG
TCCCAAGGCA
GCTCAGCAAC
TGTGGCACTT
CCCAAGTTTG
AGGAACCCCC
ATCAAAGGGG
ACGACTGTAT
GTGGTGCAGA
TGTGCATGAG
GTTGGGCCCG
GCCCTTCTGG
GTTAAGTGAA
GAGCAAAGCA
GAGCTTTCCC
AACTCCATAC
CTGAGAGTGC
CTGTGACATT
ACCTGAAAGA
AAAGGCACTT
CCCTGTCATC
AGAGAGTATC
TTGAGCCTGG
AGGGGAGTTC
ATCAACAGCA
GGGGGAAGCA
AACGTGTCCT
TTCACCAGCT
GGCAGCCTTG
AACTTACCAT
GGAAACCCAG
CAGCAAAAAA
GAGTGAAGGA
GGAGGAGGCT
TCCAAGGCAG
ATGAAAAACT
TGAGCTGGTA
AAACCATGCA
GTGTGGTCCT
TACCAGCACC
GAGGATGGGA
AGAGGCCAGA
GAAATCCCAC
TTAGGCAACC
AGGGCCAAGG
CAGGAGGGAG
CTAGAAATGC
TGTCCAGCCT
GTCCTCAGAT
CCATCTGACA
CAAAGGAAAT
TCCTCCCCTC
CCTCACCCTG
CATTGTCCCA
TGGGACAGCC
CCAGGCTCAG
AAGCTTTGAA
GGAATATCCC
CAGCCATCTG
GCAAACCTCA
CCGAAGAGGG
TTGACCCCAC
TGAGCGTGAA
GAAAGAAGAT
TCCAAGGAAC
AACCTGATCT
CCCCTCCTTG
GCACCTCCGA
GCTCTCTGCC
TCTGTCAGAA
ACGTCCTCCA
GACCAGCCAA
GTCACAAGAA
GGTACGGACT
CAAAGAAGGT
ACTGCAGTTC
CCCAGCCTCC
ACACAACCAC
TAATGTCAGT
TGGTGCTATT
TAAACTACAT
TAAGCCAAAT
CACATCCAGC
ATGCAGATAG
GAGGTCCTCT
GTTTACGCTG
GTTGGTGCAT
AAGTTTGTAC
AACCCCAACC
TTCCGGGGGT
TTCCACTAAA
GCTGCTTTGT
CCCTCCTGAG
GTCTAAGCAG
AAAGTGAGCA
AAGAGACATA
CACCATGGCC
ACAGCTCCGC
ATCCTGAGGC
AGGAAGGCTT
GTGCCATCCA
GCTCCCGCAA
GCCCCGCATG
ACAGAGGTTC
ACCTCCCTTC
ACTGGTTTCC
CCGGAAGGCC
AGAAAGATCT
AGCTAGAGTG
GAGAAGGAAG
AAGAGAGGAG
AGGGGAGCCG
GAGGAGGATG
CTCCTGCCTC
CCAGAGAGGG
GAGCCGGCGA
GGATCAAAAT
CTTCGAAGGA
GGGTACAAAT
CAAACGAAGA
GTATGTATAT
GTGCGAGGCC
GCGGCCGAGG
GAAATATGTT
TGTGAGGAGT
GTGGAATTCG
CTGCAAGAAG
CCCAGCATGC
TGAAGAAACA
CATCCGCACC
CACACTGACG
TCCGGCCCTA
TGTGTGCAAG
CACTGTCACT
TTGCTTTTAA
AACCAAAGGG
AATCTGACTA
AGCACATGAA
GTCGAAGGCC
CACAGCAAAA
AGTGCCAAGA
GACAGGGGTG
CTGGAGGAGC
TGGAAGCCGA
AGAAGGAACC
AGTGACGACC
TGTTCCAGGA
CTCGGAAGGA
CGAGAGGGTT
CAGAGGCTGT
GGAGGAGCAC
CAGTTTTCGG
ACCTGGAGGA
CTCGGACTCA
GACTCAGACC
TGGACGAAGA
CGAGGATGAG
GATGAGGAGG
AGAGCCAGGA
TGAGCTGTCC
AGACCATCCT
CAGAGGCGCC
CCCGCCTGGC
CCACCACATG
CACTGCGGGC
AGACTCCTCA
CCCATCCTGG
GCCCTCAGCC
CCCAGATGCC
CCCGCCTCTG
GCACGGAGGC
TACACGAGGC
AGCTCGGTCT
CGGAAGCTGA
GCGCCTGACA
GCCAGCAGCT
GCTCCATGTC
CAGCCAGAGC
ATGCCGGGCC
TCCCCTGGCT
GGGACCGGCC
CCTCTGGGCT
CTGTGGAGAA
AGACACAGGC
TCAGCCTTGA
GCTACAAGCC
TGTGTCCCCA
AGAAGACCGT
GGTCCCCAAG
CAAAGAAGCA
GGCAGCCGTC
CACCACTAGC
CCGCAAACAC
TCGCTAACCA
AAAACGACTC
ATCTCCCCAG
CGATGCTCCC
CGGCCCGAGA
ACCACAGGCC
TCAGCCCCAA
GCCCACCTGG
CCTGCACGTG
GACCCAGGAA
GGGGCATGGG
CGCTCTCCCT
TGTGGGTCTC
CAAGACTTCA
GCTGTCTCCT
CTCACCCTCT
GCCCCCTGGG
AAGAGAACTG
GCCCCTCGAG
CACATGTGCT
CTCCAAACTC
GAGGGTACCA
CCGACCCAGG
CCTCCCCAGA
TACTCGCCCA
CCAGGAGATG
GTCTCCAGGT
CAGGCCGAGT
CACCACCACG
GTCAGCGCCG
CCAGGGAAGT
GGGCCTTGGC
TGGGCCGGGC
AGCCCCTCAG
CGGGGGAGCA
TGGCCCAGGC
TTGGGGCTGG
ACCCACGGGT
TCTCTTCCCG
CCCGCGCCTC
TACCTCACAA
GCTCCTCAGC
AGAAGCCCAG
AGACCTGCGC
CTCCCCGTGG
CAGAAGGCCG
AGTCCCGAAG
TCCCTCCTGC
TCACCCGGCC
CTGCTCATCC
TCTCTCCTCC
CGACCCTTCT
CCGCCCTCCA
TGACTTCCAC
GGCCACATCC
TGGCCCGGAC
AGAGGAGAAC
ATCTTCAGCC
ACCTGCCTCT
GCACTCCCAG
CACTTGACCC
GTGCCCCATG
TCCCTTGATT
CCCATCGGTG
GGATCCAGAT
GGTGCAGGCC
CGGCCAGGAG
CCCACCCCAC
CCTGCTGCCA
GGGCCCACCG
CAGCCTGGGT
CAGTGGCTTC
TCCGGGGGTG
GCAGCGACCT
GACAGGGGCC
CGGGAGGCCC
AGGAGCGAGG
CCGCTGGAGT
CCCACTGAGA
GCTCGTCAGC
CTCCGTGTCG
CCTGTGGCTA
AGGTCTCCAA
ATTCACACTC
TCCTCAGAGC
TGGAGGGCGG
GGACTACCCC
AAGGAGAGGG
AGAGGACCGG
CGGAGGCCCG
GGCAGGCCTC
CTGACTGGAC
ACCCCATGGG
ACCGGGGCAC
CTGCAGAGCC
CACACCCACG
CACAGCCCCT
GCACCCCACC
CGACACCTTG
CCCCGGCCGC
CCCAGGGACG
CCGGGCAGCG
CAGTCCTGGA
GCCCCCGCTT
GGAGTCCCCG
CGTGCACCGA
CCAACCCCGA
GCCTTCTGCC
ACCCCGCCGC
TGGACCGCAG
CAGCTCTGTG
GGCTGCCTGG
CAGAGGCCTC
TGCCCGCTTC
CCAGCCCGGA
CGAGGAACCT
CTCCGGGGAA
CCCAGGACCA
GGCAGGACTC
CCCCAAGCCC
TCAGGAAGTG
GGGAGCCCAG
GGCACATCCA
CATCAGCCTG
AGGACAGGGT
TCCCCCCAAC
GCTGGATCCG
ACTACAAAGA
CCATGACGGT
GATTATAAAG
ATCATGACAT
CGACTACAAG
GATGACGATG
ACAAGTAGTG
AGGGCCCGAT
ATCTCGACAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAGCTGACG
TCCTTTCCAT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTTCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGGCCGCC
TCCCCGCCTG
GAAACGGGGG
AGGCTAACTG
AAACACGGAA
GGAGACAATA
CCGGAAGGAA
CCCGCGCTAT
GACGGCAATA
AAAAGACAGA
ATAAAACGCA
CGGGTGTTGG
GTCGTTTGTT
CATAAACGCG
GGGTTCGGTC
CCAGGGCTGG
CACTCTGTCG
ATACCCCACC
GAGACCCCAT
TGGGGCCAAT
ACGCCCGCGT
TTCTTCCTTT
TCCCCACCCC
ACCCCCCAAG
TTCGGGTGAA
GGCCCAGGGC
TCGCAGCCAA
CGTCGGGGCG
GCAGGCCCTG
CCATAGCAGA
TCTGCGCAGC
TGGGGCTCTA
GGGGGTATCC
CCACGCGCCC
TGTAGCGGCG
CATTAAGCGC
GGCGGGTGTG
GTGGTTACGC
GCAGCGTGAC
CGCTACACTT
GCCAGCGCCC
TAGCGCCCGC
TCCTTTCGCT
TTCTTCCCTT
CCTTTCTCGC
CACGTTCGCC
GGCTTTCCCC
GTCAAGCTCT
AAATCGGGGC
ATCCCTTTAG
GGTTCCGATT
TAGTGCTTTA
CGGCACCTCG
ACCCCAAAAA
ACTTGATTAG
GGTGATGGTT
CACGTAGTGG
GCCATCGCCC
TGATAGACGG
TTTTTCGCCC
TTTGACGTTG
GAGTCCACGT
TCTTTAATAG
TGGACTCTTG
TTCCAAACTG
GAACAACACT
CAACCCTATC
TCGGTCTATT
CTTTTGATTT
ATAAGGGATT
TTGGGGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TAATTCTGTG
GAATGTGTGT
CAGTTAGGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCTGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAAAGCTC
CCGGGAGCTT
GTATATCCAT
TTTCGGATCT
GATCAAGAGA
CAGGATGAGG
ATCGTTTCGC
ATGATTGAAC
AAGATGGATT
GCACGCAGGT
TCTCCGGCCG
CTTGGGTGGA
GAGGCTATTC
GGCTATGACT
GGGCACAACA
GACAATCGGC
TGCTCTGATG
CCGCCGTGTT
CCGGCTGTCA
GCGCAGGGGC
GCCCGGTTCT
TTTTGTCAAG
ACCGACCTGT
CCGGTGCCCT
GAATGAACTG
CAGGACGAGG
CAGCGCGGCT
ATCGTGGCTG
GCCACGACGG
GCGTTCCTTG
CGCAGCTGTG
CTCGACGTTG
TCACTGAAGC
GGGAAGGGAC
TGGCTGCTAT
TGGGCGAAGT
GCCGGGGCAG
GATCTCCTGT
CATCTCACCT
TGCTCCTGCC
GAGAAAGTAT
CCATCATGGC
TGATGCAATG
CGGCGGCTGC
ATACGCTTGA
TCCGGCTACC
TGCCCATTCG
ACCACCAAGC
GAAACATCGC
ATCGAGCGAG
CACGTACTCG
GATGGAAGCC
GGTCTTGTCG
ATCAGGATGA
TCTGGACGAA
GAGCATCAGG
GGCTCGCGCC
AGCCGAACTG
TTCGCCAGGC
TCAAGGCGCG
CATGCCCGAC
GGCGAGGATC
TCGTCGTGAC
CCATGGCGAT
GCCTGCTTGC
CGAATATCAT
GGTGGAAAAT
GGCCGCTTTT
CTGGATTCAT
CGACTGTGGC
CGGCTGGGTG
TGGCGGACCG
CTATCAGGAC
ATAGCGTTGG
CTACCCGTGA
TATTGCTGAA
GAGCTTGGCG
GCGAATGGGC
TGACCGCTTC
CTCGTGCTTT
ACGGTATCGC
CGCTCCCGAT
TCGCAGCGCA
TCGCCTTCTA
TCGCCTTCTT
GACGAGTTCT
TCTGAGCGGG
ACTCTGGGGT
TCGCGAAATG
ACCGACCAAG
CGACGCCCAA
CCTGCCATCA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GTATACCGTC
GACCTCTAGC
TAGAGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCAATG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGGACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTCGA
CGGATCGGGA
GATCTCCCGA
TCCCCTATGG
TCGACTCTCA
GTACAATCTG
CTCTGATGCC
GCATAGTTAA
GCCAGTATCT
GCTCCCTGCT
TGTGTGTTGG
AGGTCGCTGA
GTAGTGCGCG
AGCAAAATTT
AAGCTACAAC
AAGGCAAGGC
TTGACCGACA
ATTGCATGAA
GAATCTGCTT
AGG