名称: | pCMV-3×FLAG-HECW2(human)-Neo |
---|---|
别称: | NM_020760.4;NDHSAL; NEDL2; |
基因长: | 4719bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-T7-3×FLAG-MCS-Neo |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pCMV-3×FLAG-HECW2(human)-Neo是适用于哺乳细胞过表达的质粒。质粒带有的CMV增强子和CMV启动子可联合启动人源HECW2基因高效表达,促进HECW2基因N端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化。质粒携带Neo/G418抗性基因,可用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCCACCAT
GGACTACAAA
GACCATGACG
GTGATTATAA
AGATCATGAC
ATCGACTACA
AGGATGACGA
TGACAAGCTT
ATGGCTAGTT
CAGCCCGGGA
GCACCTGCTT
TTTGTGAGGC
GTCGAAATCC
CCAGATGCGG
TACACATTGA
GCCCAGAGAA
CCTCCAGAGC
CTTGCCGCCC
AGAGCTCCAT
GCCAGAGAAC
ATGACCCTGC
AGCGGGCCAA
CAGCGACACC
GACCTGGTGA
CTTCTGAGAG
CCGCTCCAGC
TTAACTGCCA
GCATGTACGA
GTACACGCTG
GGGCAAGCCC
AGAACCTCAT
TATCTTCTGG
GACATTAAAG
AGGAGGTGGA
CCCCAGTGAT
TGGATTGGAC
TTTATCATAT
AGATGAGAAT
TCTCCAGCCA
ACTTCTGGGA
TTCTAAGAAC
AGGGGTGTGA
CTGGAACACA
AAAAGGGCAA
ATTGTATGGA
GAATTGAGCC
TGGGCCCTAT
TTCATGGAAC
CGGAGATAAA
AATCTGTTTT
AAATATTACC
ACGGCATTAG
TGGAGCCCTG
CGAGCCACGA
CCCCCTGCAT
CACCGTGAAG
AACCCAGCTG
TGATGATGGG
GGCAGAAGGC
ATGGAGGGAG
GTGCTTCAGG
AAACCTGCAT
TCTCGAAAAC
TTGTTAGCTT
TACATTGTCA
GATCTTAGGG
CAGTTGGGCT
AAAGAAAGGG
ATGTTCTTCA
ATCCTGACCC
TTATCTTAAG
ATGTCAATTC
AGCCAGGAAA
GAAGAGCAGT
TTCCCCACCT
GTGCCCACCA
CGGGCAGGAG
AGACGGTCTA
CTATCATCAG
TAACACCACC
AATCCAATTT
GGCACCGAGA
GAAATATTCC
TTTTTTGCAC
TTCTTACTGA
TGTCTTAGAA
ATTGAAATTA
AAGACAAATT
TGCCAAGAGC
CGTCCCATCA
TCAAGCGTTT
TCTGGGGAAA
CTAACCATTC
CAGTCCAGAG
GCTGCTGGAG
CGACAAGCCA
TCGGTGATCA
AATGCTCAGC
TACAACCTTG
GCAGAAGGCT
CCCAGCTGAC
CACGTGAGTG
GGTACCTCCA
GTTTAAAGTG
GAGGTTACGT
CTTCTGTTCA
TGAAGATGCC
TCTCCAGAAG
CTGTTGGCAC
AATACTTGGA
GTCAATTCTG
TGAATGGAGA
CTTAGGTAGC
CCTTCCGATG
ACGAGGACAT
GCCAGGGAGC
CATCACGACA
GCCAGGTGTG
CTCTAATGGG
CCAGTTTCTG
AGGACAGTGC
TGCCGATGGA
ACCCCCAAGC
ATTCATTCAG
GACTAGCTCC
ACTCTGGAAA
TAGACACAGA
GGAATTAACC
TCTACGTCTT
CAAGGACCTC
ACCTCCCAGA
GGACGTCAGG
ATTCACTCAA
TGATTACTTA
GATGCTATCG
AACACAATGG
CCACTCCAGG
CCGGGGACAG
CGACCTGCTC
TGAGAGGTCC
ATGGGTGCCT
CTCCGAAACT
GAGGAGTAGT
TTTCCCACTG
ACACAAGACT
CAATGCCATG
CTTCATATTG
ATTCAGATGA
AGAAGATCAC
GAGTTCCAGC
AAGACCTGGG
TTACCCATCT
TCCTTGGAGG
AGGAGGGAGG
CCTGATCATG
TTTAGCAGGG
CATCCAGAGC
TGATGATGGA
AGCCTGACAT
CTCAGACAAA
GCTGGAGGAC
AACCCTGTTG
AGAATGAGGA
AGCCTCCACA
CACGAAGCTG
CTTCCTTTGA
GGATAAGCCA
GAAAATCTTC
CAGAGCTTGC
AGAGAGCTCC
TTACCTGCAG
GCCCAGCCCC
AGAGGAAGGT
GAAGGCGGCC
CAGAGCCTCA
ACCCAGTGCT
GACCAGGGCA
GTGCTGAACT
GTGTGGCTCT
CAAGAGGTAG
ATCAGCCCAC
AAGTGGCGCA
GACACAGGGA
CTTCCGATGC
ATCAGGAGGA
AGCCGAAGAG
CCGTCAGTGA
GACCGAGTCT
CTCGATCAGG
GCTCTGAGCC
TTCCCAGGTG
TCCTCTGAAA
CAGAACCCAG
TGATCCTGCC
AGGACAGAGA
GTGTGAGCGA
AGCCAGCACC
AGGCCTGAGG
GAGAGAGTGA
CCTGGAATGC
GCTGACAGCT
CCTGCAATGA
GAGTGTGACC
ACGCAGCTGT
CCTCTGTGGA
CACACGGTGC
TCCTCTCTTG
AGAGCGCACG
GTTTCCTGAA
ACCCCAGCCT
TTTCTTCTCA
GGAGGAGGAA
GACGGAGCCT
GTGCAGCAGA
GCCCACCAGC
AGTGGCCCTG
CCGAAGGGTC
GCAGGAATCC
GTGTGCACTG
CTGGTTCTTT
ACCTGTGGTA
CAGGTGCCCA
GTGGGGAGGA
TGAAGGGCCA
GGGGCAGAAT
CGGCCACTGT
ACCTGACCAG
GAGGAGCTGG
GGGAGGTCTG
GCAGCGGAGG
GGGAGCCTGG
AGGGAGCTGC
AGCTGCTGCC
GAGAGCCCAC
CGCAAGAAGA
AGGCAGTGCT
GGGGAGGCCC
AAGGCACCTG
TGAAGGGGCA
ACTGCCCAGG
AGGAGGGCGC
TACTGGAGGT
TCTCAAGCCA
ACGGCCACCA
GCCACTGCGA
TCACTACCTT
CAGTGCGCCA
GGATGTTAGC
CGGTACCAGA
GGGTGGACGA
GGCTCTCCCA
CCAAACTGGG
AGGCACGCAT
TGACAGCCAC
GGCAGGATCT
TCTACGTGGA
TCACGTAAAC
AGAACCACGA
CGTGGCAGCG
ACCGACAGCT
CCCCCAGCCC
CGCAGGTGCT
GCAGAGATCT
AACTCCATAC
AGCAAATGGA
GCAGCTGAAC
CGGCGATATC
AGAGTATCCG
CAGAACCATG
ACCAATGAGA
GGCCTGAGGA
AAATACCAAT
GCTATTGATG
GGGCAGGGGA
GGAAGCTGAC
TTTCACCAAG
CCAGTGCAGA
TTTCCGACGG
GAGAACATCC
TGCCTCACTC
TACCTCCAGA
TCCAGGATCA
CGTTGCTGTT
ACAGTCTCCC
CCCGTGAAGT
TCCTCATCAG
CCCAGAGTTC
TTCACCGTGC
TGCATTCTAA
CCCTAGTGCC
TACCGCATGT
TTACAAACAA
CACGTGTTTG
AAGCACATGA
TCACCAAAGT
CCGGAGGGAC
ACCCACCACT
TTGAACGCTA
CCAGCATAAC
CGCGACCTTG
TGGGATTCCT
CAACATGTTC
GCGAACAAAC
AGCTAGAGCT
GCCGCGGGGA
TGGGAAATGA
AACATGATCA
CCAGGGCAAG
GCATTCTTTG
TGGACCACAA
CTCCCGCACC
ACCACTTTCA
TTGATCCCCG
GCTCCCACTT
CAGAGCAGTA
GACCCACAAG
TGCGCTGGTT
CATCGGCAAC
ACCTGACAAG
GCAACGCAGC
CACAGTGCGG
GTGAGGTAGG
AGAAGATTCT
CGACATGCAG
GACCACCAGT
TCTTCCCAGG
CCATCCAGTA
CATTCAATAC
AGTCAGTAGG
CCACAGTACC
AGGACATGGT
TCCAGTGGCT
TACAATGACA
AGATTGTTGC
ATTTCTACGT
CAACCCAATA
TCTTTGAAAT
TCTACAGGAG
CGTCAACCTG
ATCTTACCAG
GAATCACTCA
CTCAGGGAGA
AGATCCAATT
TATCCGAACT
GAAGGGACTC
CAGGATTGGT
GCGCCTTTCA
AGCGATGCAG
ACCTTGTTAT
GTTGCTGAGC
TTATTTGAAG
AAGAGATAAT
GTCGTATGTG
CCTCCACATG
CCTTACTCCA
CCCCAGCTAC
TGTCAGTCCC
CACGTGGCTC
TCCCGTGTCA
TCTCCTCAGA
ACTCGCCAGG
TACCCAGCGT
GCCAATGCCC
GGGCTCCAGC
CCCTTACAAG
CGGGATTTCG
AAGCCAAACT
GAGGAACTTT
TACAGGAAGT
TAGAGACTAA
AGGATATGGA
CAAGGCCCAG
GGAAGTTAAA
GTTAATTATC
CGAAGAGATC
ACTTACTAGA
AGATGCTTTT
AATCAGATTA
TGGGCTACTC
CAGAAAAGAC
CTGCAGAGAA
ATAAGCTATA
TGTCACCTTC
GTTGGGGAGG
AAGGGCTGGA
TTACAGTGGG
CCTTCTAGAG
AGTTTTTCTT
CCTGGTATCC
AGAGAACTCT
TTAACCCATA
TTATGGCTTA
TTTGAATATT
CAGCCAATGA
CACATACACA
GTACAAATAA
GTCCTATGTC
TGCTTTTGTA
GACAATCACC
ATGAATGGTT
CCGATTCAGT
GGTAGGATCC
TTGGTCTTGC
ACTAATACAC
CAGTATTTGT
TGGATGCCTT
CTTCACACGG
CCCTTTTATA
AGGCTCTTCT
CAGAATTCTA
TGTGACCTGA
GTGACCTAGA
ATACCTTGAT
GAAGAGTTCC
ATCAGAGCCT
GCAGTGGATG
AAAGACAATG
ATATCCATGA
CATCCTAGAC
CTCACGTTCA
CTGTGAACGA
AGAAGTATTT
GGGCAGATAA
CTGAACGAGA
ATTAAAGCCA
GGGGGTGCCA
ATATCCCAGT
TACAGAGAAG
AACAAGAAGG
AGTACATCGA
GAGGATGGTG
AAGTGGAGGA
TTGAGAGGGG
TGTTGTACAG
CAAACAGAGA
GCTTAGTGCG
TGGCTTCTAT
GAGGTGGTGG
ATGCCAGGCT
GGTATCTGTT
TTTGATGCAA
GAGAACTGGA
ATTGGTCATC
GCAGGCACAG
CTGAAATAGA
CCTAAGTGAT
TGGAGAAACA
ACACAGAATA
TAGAGGAGGA
TACCATGACA
ATCATATTGT
AATTCGGTGG
TTCTGGGCTG
CAGTGGAAAG
ATTCAACAAT
GAACAACGAC
TAAGGTTGTT
ACAGTTTGTT
ACAGGCACAT
CCAGCATTCC
CTATGAAGGA
TTTGCTTCAC
TCCGAGGGAG
TAACGGCCCA
AGAAGATTCT
GTGTGGAGAA
ATGGGGGAAA
ATCACTGCTC
TTCCCAGAGC
GCATACATGT
TTTAACCGTC
TGGATCTGCC
TCCCTACCCA
TCCTTTTCCA
TGCTTTATGA
AAAACTGTTG
ACAGCAGTTG
AAGAAACCAG
TACTTTTGGA
CTTGAGTGAG
AATTCTGCAG
ATATCCAGCA
CAGTGGCGGC
CGCTCGAGTC
TAGAGGGCCC
GTTTAAACCC
GCTGATCAGC
CTCGACTGTG
CCTTCTAGTT
GCCAGCCATC
TGTTGTTTGC
CCCTCCCCCG
TGCCTTCCTT
GACCCTGGAA
GGTGCCACTC
CCACTGTCCT
TTCCTAATAA
AATGAGGAAA
TTGCATCGCA
TTGTCTGAGT
AGGTGTCATT
CTATTCTGGG
GGGTGGGGTG
GGGCAGGACA
GCAAGGGGGA
GGATTGGGAA
GACAATAGCA
GGCATGCTGG
GGATGCGGTG
GGCTCTATGG
CTTCTGAGGC
GGAAAGAACC
AGCTGGGGCT
CTAGGGGGTA
TCCCCACGCG
CCCTGTAGCG
GCGCATTAAG
CGCGGCGGGT
GTGGTGGTTA
CGCGCAGCGT
GACCGCTACA
CTTGCCAGCG
CCCTAGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TAGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACCCT
ATCTCGGTCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGCCTA
TTGGTTAAAA
AATGAGCTGA
TTTAACAAAA
ATTTAACGCG
AATTAATTCT
GTGGAATGTG
TGTCAGTTAG
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CAGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCTGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTT
TTGCAAAAAG
CTCCCGGGAG
CTTGTATATC
CATTTTCGGA
TCTGATCAAG
AGACAGGATG
AGGATCGTTT
CGCATGATTG
AACAAGATGG
ATTGCACGCA
GGTTCTCCGG
CCGCTTGGGT
GGAGAGGCTA
TTCGGCTATG
ACTGGGCACA
ACAGACAATC
GGCTGCTCTG
ATGCCGCCGT
GTTCCGGCTG
TCAGCGCAGG
GGCGCCCGGT
TCTTTTTGTC
AAGACCGACC
TGTCCGGTGC
CCTGAATGAA
CTGCAGGACG
AGGCAGCGCG
GCTATCGTGG
CTGGCCACGA
CGGGCGTTCC
TTGCGCAGCT
GTGCTCGACG
TTGTCACTGA
AGCGGGAAGG
GACTGGCTGC
TATTGGGCGA
AGTGCCGGGG
CAGGATCTCC
TGTCATCTCA
CCTTGCTCCT
GCCGAGAAAG
TATCCATCAT
GGCTGATGCA
ATGCGGCGGC
TGCATACGCT
TGATCCGGCT
ACCTGCCCAT
TCGACCACCA
AGCGAAACAT
CGCATCGAGC
GAGCACGTAC
TCGGATGGAA
GCCGGTCTTG
TCGATCAGGA
TGATCTGGAC
GAAGAGCATC
AGGGGCTCGC
GCCAGCCGAA
CTGTTCGCCA
GGCTCAAGGC
GCGCATGCCC
GACGGCGAGG
ATCTCGTCGT
GACCCATGGC
GATGCCTGCT
TGCCGAATAT
CATGGTGGAA
AATGGCCGCT
TTTCTGGATT
CATCGACTGT
GGCCGGCTGG
GTGTGGCGGA
CCGCTATCAG
GACATAGCGT
TGGCTACCCG
TGATATTGCT
GAAGAGCTTG
GCGGCGAATG
GGCTGACCGC
TTCCTCGTGC
TTTACGGTAT
CGCCGCTCCC
GATTCGCAGC
GCATCGCCTT
CTATCGCCTT
CTTGACGAGT
TCTTCTGAGC
GGGACTCTGG
GGTTCGAAAT
GACCGACCAA
GCGACGCCCA
ACCTGCCATC
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTC