名称: | pCMV-EGFP-ATAD2(human)-3×FLAG-Neo |
---|---|
别称: | NM_014109.4;ANCCA; CT137; PRO2000; |
基因长: | 4170bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-EGFP-2×linker-MCS-3×FLAG-Neo |
正向引物: | pEGFP-C-5' |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pCMV-EGFP-ATAD2(human)-3×FLAG-Neo是适用于哺乳细胞过表达的质粒。质粒带有的CMV增强子和CMV启动子可联合启动人源ATAD2基因高效表达,促进ATAD2基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。质粒在ATAD2基因N端携带EGFP绿色荧光蛋白,便于直观地观察目的基因表达的位置和水平等。质粒携带Neo/G418抗性基因,用于筛选出表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGCCA
CCATGGTGAG
CAAGGGCGAG
GAGCTGTTCA
CCGGGGTGGT
GCCCATCCTG
GTCGAGCTGG
ACGGCGACGT
AAACGGCCAC
AAGTTCAGCG
TGTCCGGCGA
GGGCGAGGGC
GATGCCACCT
ACGGCAAGCT
GACCCTGAAG
TTCATCTGCA
CCACCGGCAA
GCTGCCCGTG
CCCTGGCCCA
CCCTCGTGAC
CACCCTGACC
TACGGCGTGC
AGTGCTTCAG
CCGCTACCCC
GACCACATGA
AGCAGCACGA
CTTCTTCAAG
TCCGCCATGC
CCGAAGGCTA
CGTCCAGGAG
CGCACCATCT
TCTTCAAGGA
CGACGGCAAC
TACAAGACCC
GCGCCGAGGT
GAAGTTCGAG
GGCGACACCC
TGGTGAACCG
CATCGAGCTG
AAGGGCATCG
ACTTCAAGGA
GGACGGCAAC
ATCCTGGGGC
ACAAGCTGGA
GTACAACTAC
AACAGCCACA
ACGTCTATAT
CATGGCCGAC
AAGCAGAAGA
ACGGCATCAA
GGTGAACTTC
AAGATCCGCC
ACAACATCGA
GGACGGCAGC
GTGCAGCTCG
CCGACCACTA
CCAGCAGAAC
ACCCCCATCG
GCGACGGCCC
CGTGCTGCTG
CCCGACAACC
ACTACCTGAG
CACCCAGTCC
GCCCTGAGCA
AAGACCCCAA
CGAGAAGCGC
GATCACATGG
TCCTGCTGGA
GTTCGTGACC
GCCGCCGGGA
TCACTCTCGG
CATGGACGAG
CTGTACAAGG
GCGGTGGAGG
CAGTGGTGGA
GGCGGTAGCG
AATTCATGGT
GGTTCTCCGC
AGCAGCTTGG
AGCTGCACAA
CCACTCCGCG
GCCTCGGCCA
CGGGCTCCTT
GGACCTGTCC
AGTGACTTCC
TCAGTCTGGA
GCACATCGGC
CGGAGGCGGC
TCCGCTCGGC
CGGCGCGGCG
CAGAAGAAAC
CCGCGGCGAC
CACAGCCAAA
GCGGGCGATG
GGTCATCAGT
TAAGGAAGTT
GAAACCTACC
ACCGGACACG
TGCTTTAAGA
TCTTTGAGAA
AAGATGCACA
GAATTCTTCA
GATTCTAGTT
TTGAGAAGAA
TGTGGAAATA
ACGGAGCAAC
TTGCTAATGG
CAGGCATTTT
ACAAGGCAGT
TGGCCAGACA
GCAGGCTGAT
AAAAAAAAAG
AAGAGCACAG
AGAAGACAAA
GTGATTCCAG
TTACTCGGTC
ATTGAGGGCT
AGAAACATCG
TTCAAAGTAC
AGAACACTTA
CATGAAGATA
ATGGTGATGT
TGAAGTGCGT
CGAAGTTGTA
GGATTAGAAG
TCGTTATAGT
GGTGTAAACC
AGTCCATGCT
GTTTGACAAA
CTTATAACTA
ACACTGCTGA
AGCTGTACTT
CAAAAAATGG
ATGACATGAA
GAAGATGCGT
AGACAGCGAA
TGAGAGAACT
TGAAGACTTG
GGAGTGTTTA
ATGAAACAGA
AGAAAGCAAT
CTTAATATGT
ACACAAGAGG
AAAACAGAAA
GATATTCAAA
GAACTGATGA
AGAAACAACT
GATAATCAAG
AAGGCAGTGT
GGAGTCATCT
GAAGAGGGTG
AAGACCAAGA
ACATGAAGAT
GATGGTGAAG
ATGAAGATGA
TGAAGATGAT
GATGATGATG
ACGATGATGA
TGATGATGAT
GATGATGAAG
ATGATGAAGA
TGAAGAAGAT
GGAGAAGAAG
AGAATCAGAA
GCGATATTAT
CTTAGACAGA
GAAAAGCTAC
TGTTTACTAT
CAGGCTCCAT
TGGAAAAACC
TCGTCACCAG
AGAAAGCCCA
ACATATTTTA
TAGTGGCCCA
GCTTCTCCTG
CAAGACCAAG
ATACCGATTA
TCTTCCGCAG
GACCAAGAAG
TCCTTACTGT
AAACGAATGA
ACAGGCGAAG
GCATGCAATC
CACAGTAGTG
ACTCGACTTC
ATCTTCCTCC
TCTGAAGATG
AACAGCACTT
TGAGAGGCGG
AGGAAAAGGA
GTCGTAATAG
GGCTATCAAT
AGGTGCCTCC
CACTAAATTT
TCGGAAAGAT
GAATTAAAAG
GCATTTATAA
AGATCGAATG
AAAATTGGAG
CAAGCCTTGC
CGATGTTGAT
CCAATGCAAC
TAGATTCTTC
AGTACGATTT
GATAGTGTTG
GTGGCCTGTC
TAATCATATA
GCAGCTCTAA
AAGAGATGGT
GGTGTTTCCA
TTACTTTATC
CAGAAGTCTT
TGAAAAATTT
AAAATTCAAC
CCCCAAGAGG
TTGTTTGTTT
TATGGGCCAC
CTGGAACTGG
AAAGACTCTG
GTTGCCAGAG
CACTTGCCAA
TGAGTGCAGT
CAAGGGGATA
AAAGAGTAGC
ATTTTTCATG
AGGAAAGGTG
CTGATTGTCT
AAGTAAATGG
GTAGGAGAAT
CTGAAAGACA
GCTACGATTG
CTGTTTGATC
AGGCCTATCA
GATGCGCCCA
TCAATTATTT
TTTTTGACGA
AATTGATGGT
CTGGCTCCAG
TACGGTCAAG
CAGGCAAGAT
CAGATTCACA
GTTCTATTGT
TTCCACCCTG
CTAGCTCTTA
TGGATGGATT
GGACAGCAGA
GGGGAAATTG
TGGTCATTGG
TGCTACGAAC
AGGCTAGATT
CTATAGATCC
TGCTTTACGA
AGGCCTGGTC
GCTTTGATAG
AGAATTCCTC
TTTAGCCTGC
CTGATAAAGA
GGCTCGAAAA
GAGATTCTAA
AGATTCACAC
CAGGGATTGG
AATCCCAAAC
CACTGGACAC
ATTTTTAGAA
GAGCTAGCAG
AAAACTGTGT
TGGATACTGT
GGAGCAGATA
TTAAATCAAT
ATGTGCTGAA
GCTGCTTTAT
GTGCTTTACG
ACGACGCTAC
CCACAGATCT
ATACCACTAG
TGAGAAACTG
CAGTTGGATC
TCTCTTCAAT
TAATATCTCA
GCTAAGGATT
TCGAGGTAGC
TATGCAAAAG
ATGATACCAG
CCTCCCAAAG
AGCTGTGACA
TCACCTGGGC
AGGCACTGTC
CACCGTTGTG
AAACCACTCC
TGCAAAACAC
TGTTGACAAG
ATTTTAGAAG
CCCTGCAGAG
AGTATTTCCA
CATGCAGAAT
TCAGAACAAA
TAAAACATTA
GACTCAGATA
TTTCTTGTCC
TCTGCTAGAA
AGTGACTTGG
CTTACAGTGA
TGATGATGTT
CCATCAGTTT
ATGAAAATGG
ACTTTCTCAG
AAATCTTCTC
ATAAGGCAAA
AGACAATTTT
AATTTTCTTC
ATTTGAATAG
AAATGCTTGT
TACCAACCTA
TGTCTTTTCG
ACCAAGAATA
TTGATAGTAG
GAGAACCAGG
ATTTGGGCAA
GGTTCTCACT
TGGCACCAGC
TGTCATTCAT
GCTTTGGAAA
AGTTTACTGT
ATATACATTA
GACATTCCTG
TTCTTTTTGG
AGTTAGTACT
ACATCCCCTG
AAGAAACATG
TGCCCAGGTG
ATTCGTGAAG
CTAAGAGAAC
AGCACCAAGT
ATAGTGTATG
TTCCTCATAT
CCACGTGTGG
TGGGAAATAG
TTGGACCGAC
ACTTAAAGCC
ACATTTACCA
CATTATTACA
GAATATTCCT
TCATTTGCTC
CAGTTTTACT
ACTTGCAACT
TCTGACAAAC
CCCATTCCGC
TTTGCCAGAA
GAGGTGCAAG
AATTGTTTAT
CCGTGATTAT
GGAGAGATTT
TTAATGTCCA
GTTACCGGAT
AAAGAAGAAC
GGACAAAATT
TTTTGAAGAT
TTAATTCTAA
AACAAGCTGC
TAAGCCTCCT
ATATCAAAAA
AGAAAGCAGT
TTTGCAGGCT
TTGGAGGTAC
TCCCAGTAGC
ACCACCACCT
GAGCCAAGAT
CACTGACAGC
AGAAGAAGTG
AAACGACTAG
AAGAACAAGA
AGAAGATACA
TTTAGAGAAC
TGAGGATTTT
CTTAAGAAAT
GTTACACATA
GGCTTGCTAT
TGACAAGCGA
TTCCGAGTGT
TTACTAAGCC
TGTTGACCCT
GATGAGGTTC
CTGATTATGT
CACTGTAATA
AAGCAACCAA
TGGACCTTTC
ATCTGTAATC
AGTAAAATTG
ATCTACACAA
GTATCTGACT
GTGAAAGACT
ATTTGAGAGA
TATTGATCTA
ATCTGTAGTA
ATGCCTTAGA
ATACAATCCA
GATAGAGATC
CTGGAGATCG
TCTTATTAGG
CATAGAGCCT
GTGCTTTAAG
AGATACTGCC
TATGCCATAA
TTAAAGAAGA
ACTTGATGAA
GACTTTGAGC
AGCTCTGTGA
AGAAATTCAG
GAATCTAGAA
AGAAAAGAGG
TTGTAGCTCC
TCCAAATATG
CCCCGTCTTA
CTACCATGTG
ATGCCAAAGC
AAAATTCCAC
TCTTGTTGGT
GATAAAAGAT
CAGACCCAGA
GCAGAATGAA
AAGCTAAAGA
CACCGAGTAC
TCCTGTGGCT
TGCAGCACTC
CTGCTCAGTT
GAAGAGGAAA
ATTCGCAAAA
AGTCAAACTG
GTACTTAGGC
ACCATAAAAA
AGCGAAGGAA
GATTTCACAG
GCAAAGGATG
ATAGCCAGAA
TGCCATAGAT
CACAAAATTG
AGAGTGATAC
AGAGGAAACT
CAAGACACAA
GTGTAGATCA
TAATGAGACC
GGAAACACAG
GAGAGTCTTC
GGTGGAAGAA
AATGAAAAAC
AGCAAAATGC
CTCTGAAAGC
AAACTGGAAT
TGAGAAATAA
TTCAAATACT
TGTAATATAG
AGAATGAGCT
TGAAGACTCT
AGGAAGACTA
CAGCATGTAC
AGAATTGAGA
GACAAGATTG
CTTGTAATGG
AGATGCTTCT
AGCTCTCAGA
TAATACATAT
TTCTGATGAA
AATGAAGGAA
AAGAAATGTG
TGTTCTGCGA
ATGACTCGAG
CTAGACGTTC
CCAGGTAGAA
CAGCAGCAGC
TCATCACTGT
TGAAAAGGCT
TTGGCAATTC
TTTCTCAGCC
TACACCCTCA
CTTGTTGTGG
ATCATGAGCG
ATTAAAAAAT
CTTTTGAAGA
CTGTTGTTAA
AAAAAGTCAA
AACTACAACA
TATTTCAGTT
GGAAAATTTG
TATGCAGTAA
TCAGCCAATG
TATTTATCGG
CATCGCAAGG
ACCATGATAA
AACATCACTT
ATTCAGAAAA
TGGAGCAAGA
GGTAGAAAAC
TTCAGTTGTT
CCAGACTCGA
GGACTACAAA
GACCATGACG
GTGATTATAA
AGATCATGAC
ATCGACTACA
AGGATGACGA
TGACAAGTAG
TGAGGGCCCG
TTTAAACCCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
ACAATAGCAG
GCATGCTGGG
GATGCGGTGG
GCTCTATGGC
TTCTGAGGCG
GAAAGAACCA
GCTGGGGCTC
TAGGGGGTAT
CCCCACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTAATTCTG
TGGAATGTGT
GTCAGTTAGG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
AGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCATAGTCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCTGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TCCCGGGAGC
TTGTATATCC
ATTTTCGGAT
CTGATCAAGA
GACAGGATGA
GGATCGTTTC
GCATGATTGA
ACAAGATGGA
TTGCACGCAG
GTTCTCCGGC
CGCTTGGGTG
GAGAGGCTAT
TCGGCTATGA
CTGGGCACAA
CAGACAATCG
GCTGCTCTGA
TGCCGCCGTG
TTCCGGCTGT
CAGCGCAGGG
GCGCCCGGTT
CTTTTTGTCA
AGACCGACCT
GTCCGGTGCC
CTGAATGAAC
TGCAGGACGA
GGCAGCGCGG
CTATCGTGGC
TGGCCACGAC
GGGCGTTCCT
TGCGCAGCTG
TGCTCGACGT
TGTCACTGAA
GCGGGAAGGG
ACTGGCTGCT
ATTGGGCGAA
GTGCCGGGGC
AGGATCTCCT
GTCATCTCAC
CTTGCTCCTG
CCGAGAAAGT
ATCCATCATG
GCTGATGCAA
TGCGGCGGCT
GCATACGCTT
GATCCGGCTA
CCTGCCCATT
CGACCACCAA
GCGAAACATC
GCATCGAGCG
AGCACGTACT
CGGATGGAAG
CCGGTCTTGT
CGATCAGGAT
GATCTGGACG
AAGAGCATCA
GGGGCTCGCG
CCAGCCGAAC
TGTTCGCCAG
GCTCAAGGCG
CGCATGCCCG
ACGGCGAGGA
TCTCGTCGTG
ACCCATGGCG
ATGCCTGCTT
GCCGAATATC
ATGGTGGAAA
ATGGCCGCTT
TTCTGGATTC
ATCGACTGTG
GCCGGCTGGG
TGTGGCGGAC
CGCTATCAGG
ACATAGCGTT
GGCTACCCGT
GATATTGCTG
AAGAGCTTGG
CGGCGAATGG
GCTGACCGCT
TCCTCGTGCT
TTACGGTATC
GCCGCTCCCG
ATTCGCAGCG
CATCGCCTTC
TATCGCCTTC
TTGACGAGTT
CTTCTGAGCG
GGACTCTGGG
GTTCGAAATG
ACCGACCAAG
CGACGCCCAA
CCTGCCATCA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GTATACCGTC
GACCTCTAGC
TAGAGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTC