名称: | pLV3-CMV-LAMA4(human)-3×FLAG-CopGFP-Puro |
---|---|
别称: | NM_001105206.3;CMD1JJ; LAMA3; LAMA4*-1 |
基因长: | 5469bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-LAMA4(human)-3×FLAG-CopGFP-Puro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动人源LAMA4基因转录,促进LAMA4基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带的CopGFP绿色荧光蛋白用于观察目的基因的表达和定位情况。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGG
CTTTGAGCTC
AGCCTGGCGC
TCGGTTCTGC
CTCTGTGGCT
CCTCTGGAGC
GCTGCCTGCT
CCCGCGCCGC
GTCCGGGGAC
GACAACGCTT
TTCCTTTTGA
CATTGAAGGG
AGCTCAGCGG
TTGGCAGGCA
AGACCCGCCT
GAGACGAGCG
AACCCCGCGT
GGCTCTGGGA
CGCCTGCCGC
CTGCGGCCGA
GAAATGCAAT
GCTGGATTCT
TTCACACCCT
GTCGGGAGAA
TGTGTGCCCT
GCGACTGTAA
TGGCAATTCC
AACGAGTGTT
TGGACGGCTC
AGGATACTGT
GTGCACTGCC
AGCGGAACAC
AACAGGAGAG
CACTGTGAAA
AGTGTCTGGA
TGGTTATATC
GGAGATTCCA
TCAGGGGAGC
ACCCCAATTC
TGCCAGCCGT
GCCCCTGTCC
CCTGCCCCAC
TTGGCCAATT
TTGCAGAATC
CTGCTATAGG
AAAAATGGAG
CTGTTCGGTG
CATTTGTAAC
GAAAATTATG
CTGGACCTAA
CTGTGAAAGA
TGTGCTCCCG
GTTACTATGG
AAACCCCTTA
CTCATTGGAA
GCACCTGTAA
GAAATGTGAC
TGCAGTGGAA
ATTCAGATCC
CAACCTGATC
TTTGAAGATT
GTGATGAAGT
CACTGGCCAG
TGTAGGAATT
GCTTACGCAA
CACCACCGGA
TTCAAGTGTG
AACGTTGCGC
TCCTGGCTAC
TATGGGGACG
CCAGGATAGC
CAAGAACTGT
GCAGTGTGCA
ACTGCGGGGG
AGGCCCATGT
GACAGTGTAA
CCGGAGAATG
CTTGGAAGAA
GGTTTTGAAC
CCCCTACAGG
CATGGACTGC
CCAACCATAA
GCTGTGATAA
GTGCGTCTGG
GACCTGACTG
ATGACCTGCG
GTTAGCAGCG
CTCTCCATCG
AGGAAGGCAA
ATCCGGGGTG
CTGAGCGTAT
CCTCTGGGGC
CGCCGCTCAT
AGGCACGTGA
ATGAAATCAA
CGCCACCATC
TACCTCCTCA
AAACAAAATT
GTCAGAAAGA
GAAAACCAAT
ACGCCCTAAG
AAAGATACAA
ATCAACAATG
CTGAGAACAC
GATGAAAAGC
CTTCTGTCTG
ACGTAGAGGA
ATTAGTTGAA
AAGGAAAATC
AAGCCTCCAG
AAAAGGACAA
CTTGTTCAGA
AGGAAAGCAT
GGACACCATT
AACCACGCAA
GTCAGCTGGT
AGAGCAAGCC
CATGATATGA
GGGATAAAAT
CCAAGAGATC
AACAACAAGA
TGCTCTATTA
TGGGGAAGAG
CATGAACTTA
GCCCCAAGGA
AATCTCTGAG
AAGCTGGTGT
TGGCCCAGAA
GATGCTTGAA
GAGATTAGAA
GCCGTCAACC
ATTTTTCACC
CAACGGGAGC
TCGTGGATGA
GGAGGCAGAT
GAGGCTTACG
AACTACTGAG
CCAGGCTGAG
AGCTGGCAGC
GGCTGCACAA
TGAGACCCGC
ACTCTGTTTC
CTGTCGTCCT
GGAGCAGCTG
GATGACTACA
ATGCTAAGTT
GTCAGATCTC
CAGGAAGCAC
TTGACCAGGC
CCTTAACTAT
GTCAGGGATG
CCGAAGACAT
GAACAGGGCC
ACAGCAGCCA
GGCAGCGGGA
CCATGAGAAA
CAACAGGAAA
GAGTGAGGGA
ACAAATGGAA
GTGGTGAACA
TGTCTCTGAG
CACATCTGCG
GACTCTCTGA
CAACACCTCG
TCTAACTCTT
TCAGAACTTG
ATGATATAAT
AAAGAATGCG
TCAGGGATTT
ATGCAGAAAT
AGATGGAGCC
AAAAGTGAAC
TACAAGTAAA
ACTATCTAAC
CTAAGTAACC
TCAGCCATGA
TTTAGTCCAA
GAAGCTATTG
ACCATGCACA
GGACCTTCAA
CAAGAAGCTA
ATGAATTGAG
CAGGAAGTTG
CACAGTTCAG
ATATGAACGG
GCTGGTACAG
AAGGCTTTGG
ATGCATCAAA
TGTCTATGAA
AATATTGTTA
ATTATGTTAG
TGAAGCCAAT
GAAACAGCAG
AATTTGCTTT
GAACACCACT
GACCGAATTT
ATGATGCGGT
GAGTGGGATT
GATACTCAAA
TCATTTACCA
TAAAGATGAA
AGTGAGAACC
TCCTCAATCA
AGCCAGAGAA
CTGCAAGCAA
AGGCAGAGTC
TAGCAGTGAT
GAAGCAGTGG
CTGACACTAG
CAGGCGTGTG
GGTGGAGCCC
TAGCAAGGAA
AAGTGCCCTT
AAAACCAGAC
TCAGTGATGC
CGTTAAGCAA
CTACAAGCAG
CAGAGAGAGG
GGATGCCCAG
CAGCGCCTGG
GGCAGTCTAG
ACTGATCACC
GAGGAAGCCA
ACAGGACGAC
GATGGAGGTG
CAGCAGGCCA
CTGCCCCCAT
GGCCAACAAT
CTAACCAACT
GGTCACAGAA
TCTTCAACAT
TTTGACTCTT
CTGCTTACAA
CACTGCAGTG
AACTCTGCTA
GGGATGCAGT
AAGAAATCTG
ACCGAGGTTG
TCCCTCAGCT
CCTGGATCAG
CTTCGTACGG
TTGAGCAGAA
GCGACCTGCA
AGCAACGTTT
CTGCCAGCAT
CCAGAGGATC
CGAGAGCTCA
TTGCTCAGAC
CAGAAGTGTT
GCCAGCAAGA
TCCAAGTCTC
CATGATGTTT
GATGGCCAGT
CAGCTGTGGA
AGTGCACTCG
AGAACCAGTA
TGGATGACTT
AAAGGCCTTC
ACGTCTCTGA
GCCTGTACAT
GAAACCCCCT
GTGAAGCGGC
CGGAACTGAC
CGAGACTGCA
GATCAGTTTA
TCCTGTACCT
CGGAAGCAAA
AACGCCAAAA
AAGAGTATAT
GGGTCTTGCA
ATCAAAAATG
ATAATCTGGT
ATACGTCTAT
AATTTGGGAA
CTAAAGATGT
GGAGATTCCC
CTGGACTCCA
AGCCCGTCAG
TTCCTGGCCT
GCTTACTTCA
GCATTGTCAA
GATTGAAAGG
GTGGGAAAAC
ATGGAAAGGT
GTTTTTAACA
GTCCCGAGTC
TAAGTAGCAC
AGCAGAGGAA
AAGTTCATTA
AAAAGGGGGA
ATTTTCGGGA
GATGACTCTC
TGCTGGACCT
GGACCCTGAG
GACACAGTGT
TTTATGTTGG
TGGAGTGCCT
TCCAACTTCA
AGCTCCCTAC
CAGCTTAAAC
CTGCCTGGCT
TTGTTGGCTG
CCTGGAACTG
GCCACTTTGA
ATAATGATGT
GATCAGCTTG
TACAACTTTA
AGCACATCTA
TAATATGGAC
CCCTCCACAT
CAGTGCCATG
TGCCCGAGAT
AAGCTGGCCT
TCACTCAGAG
TCGGGCTGCC
AGTTACTTCT
TCGATGGCTC
CGGTTATGCC
GTGGTGAGAG
ACATCACAAG
GAGAGGGAAA
TTTGGTCAGG
TGACTCGCTT
TGACATAGAA
GTTCGAACAC
CAGCTGACAA
CGGCCTTATT
CTCCTGATGG
TCAATGGAAG
TATGTTTTTC
AGACTGGAAA
TGCGCAATGG
TTACCTACAT
GTGTTCTATG
ATTTTGGATT
CAGCGGTGGC
CCTGTGCATC
TTGAAGATAC
GTTAAAGAAA
GCTCAAATTA
ATGATGCAAA
ATACCATGAG
ATCTCAATCA
TTTACCACAA
TGATAAGAAA
ATGATCTTGG
TAGTTGACAG
AAGGCATGTC
AAGAGCATGG
ATAATGAAAA
GATGAAAATA
CCTTTTACAG
ATATATACAT
TGGAGGAGCT
CCTCCAGAAA
TCTTACAATC
CAGGGCCCTC
AGAGCACACC
TTCCCCTAGA
TATCAACTTC
AGAGGATGCA
TGAAGGGCTT
CCAGTTCCAA
AAGAAGGACT
TCAATTTACT
GGAGCAGACA
GAAACCCTGG
GAGTTGGTTA
TGGATGCCCA
GAAGACTCAC
TTATATCTCG
CAGAGCATAT
TTCAATGGAC
AGAGCTTCAT
TGCTTCAATT
CAGAAAATAT
CTTTCTTTGA
TGGCTTTGAA
GGAGGTTTTA
ATTTCCGAAC
ATTACAACCA
AATGGGTTAC
TATTCTATTA
TGCTTCAGGG
TCAGACGTGT
TCTCCATCTC
ACTGGATAAT
GGTACTGTCA
TCATGGATGT
AAAGGGAATC
AAAGTTCAGT
CAGTAGATAA
GCAGTACAAT
GATGGGCTGT
CCCACTTCGT
CATTAGCTCT
GTCTCACCCA
CAAGATATGA
ACTGATAGTA
GATAAAAGCA
GAGTTGGGAG
TAAGAATCCT
ACCAAAGGGA
AAATAGAACA
GACACAAGCA
AGTGAAAAGA
AGTTTTACTT
CGGTGGCTCA
CCAATCAGTG
CTCAGTATGC
TAATTTCACT
GGCTGCATAA
GTAATGCCTA
CTTTACCAGG
GTGGATAGAG
ATGTGGAGGT
TGAAGATTTC
CAACGGTATA
CTGAAAAGGT
CCACACTTCT
CTTTATGAGT
GTCCCATTGA
GTCTTCACCA
TTGTTTCTCC
TCCATAAAAA
AGGAAAAAAT
TTATCCAAGC
CTAAAGCAAG
TCAGAATAAA
AAGGGAGGGA
AAAGTAAAGA
TGCACCTTCA
TGGGATCCTG
TTGCTCTGAA
ACTCCCAGAG
CGGAATACTC
CAAGAAACTC
TCATTGCCAC
CTTTCCAACA
GCCCTAGAGC
AATAGAGCAC
GCCTATCAAT
ATGGAGGAAC
AGCCAACAGC
CGCCAAGAGT
TTGAACACTT
AAAAGGAGAT
TTTGGTGCCA
AATCTCAGTT
TTCCATTCGT
CTGAGAACTC
GTTCCTCCCA
TGGCATGATC
TTCTATGTCT
CAGATCAAGA
AGAGAATGAC
TTCATGACTC
TATTTTTGGC
CCATGGCCGC
TTGGTTTACA
TGTTTAATGT
TGGTCACAAA
AAACTGAAGA
TTAGAAGCCA
GGAGAAATAC
AATGATGGCC
TGTGGCATGA
TGTGATATTT
ATTCGAGAAA
GGAGCAGTGG
CCGACTGGTA
ATTGATGGTC
TCCGAGTCCT
AGAAGAAAGT
CTTCCTCCTA
CTGAAGCTAC
CTGGAAAATC
AAGGGTCCCA
TTTATTTGGG
AGGTGTGGCT
CCTGGAAAGG
CTGTGAAAAA
TGTTCAGATT
AACTCCATCT
ACAGTTTTAG
TGGCTGTCTC
AGCAATCTCC
AGCTCAATGG
GGCCTCCATC
ACCTCTGCTT
CTCAGACATT
CAGTGTGACC
CCTTGCTTTG
AAGGCCCCAT
GGAAACAGGA
ACTTACTTTT
CAACAGAAGG
AGGATACGTG
GTTCTAGATG
AATCTTTCAA
TATTGGATTG
AAGTTTGAAA
TTGCATTTGA
AGTCCGTCCC
AGAAGCAGTT
CCGGAACCCT
GGTCCACGGC
CACAGTGTCA
ATGGGGAGTA
CCTAAATGTT
CACATGAAAA
ATGGACAGGT
CATAGTGAAA
GTCAATAATG
GCATCAGAGA
TTTTTCCACC
TCAGTTACAC
CCAAGCAGAG
TCTCTGTGAT
GGCAGATGGC
ACAGAATTAC
AGTTATTAGA
GATTCTAATG
TGGTTCAGTT
GGATGTGGAC
TCTGAAGTGA
ACCATGTGGT
TGGACCCCTG
AATCCAAAAC
CAATTGATCA
CAGGGAGCCT
GTGTTTGTTG
GAGGTGTTCC
AGAATCTCTA
CTGACACCAC
GCTTGGCCCC
CAGCAAACCC
TTCACAGGCT
GCATACGCCA
CTTTGTGATT
GATGGACACC
CAGTGAGCTT
CAGTAAAGCA
GCCCTGGTCA
GCGGCGCCGT
AAGCATCAAC
TCCTGTCCAG
CAGCCGGATC
CGACTACAAA
GACCATGACG
GTGATTATAA
AGATCATGAC
ATCGACTACA
AGGATGACGA
TGACAAGTAG
CGGCCGCGAA
GGATCTGCGA
TCGCTCCGGT
GCCCGTCAGT
GGGCAGAGCG
CACATCGCCC
ACAGTCCCCG
AGAAGTTGGG
GGGAGGGGTC
GGCAATTGAA
CGGGTGCCTA
GAGAAGGTGG
CGCGGGGTAA
ACTGGGAAAG
TGATGTCGTG
TACTGGCTCC
GCCTTTTTCC
CGAGGGTGGG
GGAGAACCGT
ATATAAGTGC
AGTAGTCGCC
GTGAACGTTC
TTTTTCGCAA
CGGGTTTGCC
GCCAGAACAC
AGCTGAAGCT
TCGAGGGGCT
CGCATCTCTC
CTTCACGCGC
CCGCCGCCCT
ACCTGAGGCC
GCCATCCACG
CCGGTTGAGT
CGCGTTCTGC
CGCCTCCCGC
CTGTGGTGCC
TCCTGAACTG
CGTCCGCCGT
CTAGGTAAGT
TTAAAGCTCA
GGTCGAGACC
GGGCCTTTGT
CCGGCGCTCC
CTTGGAGCCT
ACCTAGACTC
AGCCGGCTCT
CCACGCTTTG
CCTGACCCTG
CTTGCTCAAC
TCTACGTCTT
TGTTTCGTTT
TCTGTTCTGC
GCCGTTACAG
ATCCAAGCTG
TGACCGGCGC
CTACGCTAGA
CGCCACCATG
GAGAGCGACG
AGAGCGGCCT
GCCCGCCATG
GAGATCGAGT
GCCGCATCAC
CGGCACCCTG
AACGGCGTGG
AGTTCGAGCT
GGTGGGCGGC
GGAGAGGGCA
CCCCCAAGCA
GGGCCGCATG
ACCAACAAGA
TGAAGAGCAC
CAAAGGCGCC
CTGACCTTCA
GCCCCTACCT
GCTGAGCCAC
GTGATGGGCT
ACGGCTTCTA
CCACTTCGGC
ACCTACCCCA
GCGGCTACGA
GAACCCCTTC
CTGCACGCCA
TCAACAACGG
CGGCTACACC
AACACCCGCA
TCGAGAAGTA
CGAGGACGGC
GGCGTGCTGC
ACGTGAGCTT
CAGCTACCGC
TACGAGGCCG
GCCGCGTGAT
CGGCGACTTC
AAGGTGGTGG
GCACCGGCTT
CCCCGAGGAC
AGCGTGATCT
TCACCGACAA
GATCATCCGC
AGCAACGCCA
CCGTGGAGCA
CCTGCACCCC
ATGGGCGATA
ACGTGCTGGT
GGGCAGCTTC
GCCCGCACCT
TCAGCCTGCG
CGACGGCGGC
TACTACAGCT
TCGTGGTGGA
CAGCCACATG
CACTTCAAGA
GCGCCATCCA
CCCCAGCATC
CTGCAGAACG
GGGGCCCCAT
GTTCGCCTTC
CGCCGCGTGG
AGGAGCTGCA
CAGCAACACC
GAGCTGGGCA
TCGTGGAGTA
CCAGCACGCC
TTCAAGACCC
CCATCGCCTT
CGCCAGATCC
CGCGCTCAGT
CGTCCAATTC
TGCCGTGGAC
GGCACCGCCG
GACCCGGCTC
CACCGGATCT
CGCGAGGGCA
GAGGAAGTCT
TCTAACATGC
GGTGACGTGG
AGGAGAATCC
CGGCCCTATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCAG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
TCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGCGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTGAAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAATCATCGT
CCTTTCCTTG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCCTGG
TACCTTTAAG
ACCAATGACT
TACAAGGCAG
CTGTAGATCT
TAGCCACTTT
TTAAAAGAAA
AGGGGGGACT
GGAAGGGCTA
ATTCACTCCC
AACGAAGATA
AGATCTGCTT
TTTGCTTGTA
CTGGGTCTCT
CTGGTTAGAC
CAGATCTGAG
CCTGGGAGCT
CTCTGGCTAA
CTAGGGAACC
CACTGCTTAA
GCCTCAATAA
AGCTTGCCTT
GAGTGCTTCA
AGTAGTGTGT
GCCCGTCTGT
TGTGTGACTC
TGGTAACTAG
AGATCCCTCA
GACCCTTTTA
GTCAGTGTGG
AAAATCTCTA
GCAGTAGTAG
TTCATGTCAT
CTTATTATTC
AGTATTTATA
ACTTGCAAAG
AAATGAATAT
CAGAGAGTGA
GAGGAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGCTCT
AGCTATCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCGGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGACTTTT
GCAGAGACCA
AATTCGTAAT
CATGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCATACTCT
TCCTTTTTCA
ATATTATTGA
AGCATTTATC
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGACGT
CTAAGAAACC
ATTATTATCA
TGACATTAAC
CTATAAAAAT
AGGCGTATCA
CGAGGCCCTT
TCGTCTCGCG
CGTTTCGGTG
ATGACGGTGA
AAACCTCTGA
CACATGCAGC
TCCCGGAGAC
GGTCACAGCT
TGTCTGTAAG
CGGATGCCGG
GAGCAGACAA
GCCCGTCAGG
GCGCGTCAGC
GGGTGTTGGC
GGGTGTCGGG
GCTGGCTTAA
CTATGCGGCA
TCAGAGCAGA
TTGTACTGAG
AGTGCACCAT
ATGCGGTGTG
AAATACCGCA
CAGATGCGTA
AGGAGAAAAT
ACCGCATCAG
GCGCCATTCG
CCATTCAGGC
TGCGCAACTG
TTGGGAAGGG
CGATCGGTGC
GGGCCTCTTC
GCTATTACGC
CAGCTGGCGA
AAGGGGGATG
TGCTGCAAGG
CGATTAAGTT
GGGTAACGCC
AGGGTTTTCC
CAGTCACGAC
GTTGTAAAAC
GACGGCCAGT
GCCAAGCTG