名称: | pDC316-Enpp2(mouse)-3×FLAG-mCMV-EGFP |
---|---|
别称: | NM_001136077.3;ATX; E-NPP 2; lysoPLD; Npps2; PD-Ialpha;Pdnp2; |
基因长: | 2742bp |
质粒宿主: | 哺乳细胞,腺病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pDC316-mCMV-EGFP |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pDC316-Enpp2(mouse)-3×FLAG-mCMV-EGFP是适用于哺乳细胞过表达的质粒。质粒带有的CMV增强子和CMV启动子可联合启动小鼠源Enpp2基因,促进Enpp2基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。此外,质粒带有包装信号Ad5 Ψ和反向末端重复序列 ITRs 元件可用于腺病毒包装,便于基因稳定整合至宿主细胞。质粒携带EGFP绿色荧光蛋白可以观察目的基因的表达和定位情况。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTCATCAATA
ATATACCTTA
TTTTGGATTG
AAGCCAATAT
GATAATGAGG
GGGTGGAGTT
TGTGACGTGG
CGCGGGGCGT
GGGAACGGGG
CGGGTGACGT
AGTAGTGTGG
CGGAAGTGTG
ATGTTGCAAG
TGTGGCGGAA
CACATGTAAG
CGACGGATGT
GGCAAAAGTG
ACGTTTTTGG
TGTGCGCCGG
TGTACACAGG
AAGTGACAAT
TTTCGCGCGG
TTTTAGGCGG
ATGTTGTAGT
AAATTTGGGC
GTAACCGAGT
AAGATTTGGC
CATTTTCGCG
GGAAAACTGA
ATAAGAGGAA
GTGAAATCTG
AATAATTTTG
TGTTACTCAT
AGCGCGTAAT
ATTTGTCTAG
GGCCGCGGGG
ACTTTGACCG
TTTACGTGGA
GACTCGCCCA
GGTGTTTTTC
TCAGGTGTTT
TCCGCGTTCC
GGGTCAAAGT
TGGCGTTTTA
TTATTATAGT
CAGTCTAGAG
ATATACTGAG
TCATTAGGGA
CTTTCCAATG
GGTTTTGCCC
AGTACATAAG
GTCAATAGGG
GTGAATCAAC
AGGAAAGTCC
CATTGGAGCC
AAGTACACTG
AGTCAATAGG
GACTTTCCAT
TGGGTTTTGC
CCAGTACAAA
AGGTCAATAG
GGGGTGAGTC
AATGGGTTTT
TCCCATTATT
GGCACGTACA
TAAGGTCAAT
AGGGGTGAGT
CATTGGGTTT
TTCCAGCCAA
TTTAATTAAA
ACGCCATGTA
CTTTCCCACC
ATTGACGTCA
ATGGGCTATT
GAAACTAATG
CAACGTGACC
TTTAAACGGT
ACTTTCCCAT
AGCTGATTAA
TGGGAAAGTA
CCGTTCTCGA
GCCAATACAC
GTCAATGGGA
AGTGAAAGGG
CAGCCAAAAC
GTAACACCGC
CCCGGTTTTC
CCCTGGAAAT
TCCATATTGG
CACGCATTCT
ATTGGCTGAG
CTGCGTTCTA
CGTGGGTATA
AGAGGCGCGA
CCAGCGTCGG
TACCGTCGCA
GTCTTCGGTC
TGACCACCGT
AGAACGCAGA
TCGAATTCAT
GGTGAGCAAG
GGCGAGGAGC
TGTTCACCGG
GGTGGTGCCC
ATCCTGGTCG
AGCTGGACGG
CGACGTAAAC
GGCCACAAGT
TCAGCGTGTC
CGGCGAGGGC
GAGGGCGATG
CCACCTACGG
CAAGCTGACC
CTGAAGTTCA
TCTGCACCAC
CGGCAAGCTG
CCCGTGCCCT
GGCCCACCCT
CGTGACCACC
CTGACCTACG
GCGTGCAGTG
CTTCAGCCGC
TACCCCGACC
ACATGAAGCA
GCACGACTTC
TTCAAGTCCG
CCATGCCCGA
AGGCTACGTC
CAGGAGCGCA
CCATCTTCTT
CAAGGACGAC
GGCAACTACA
AGACCCGCGC
CGAGGTGAAG
TTCGAGGGCG
ACACCCTGGT
GAACCGCATC
GAGCTGAAGG
GCATCGACTT
CAAGGAGGAC
GGCAACATCC
TGGGGCACAA
GCTGGAGTAC
AACTACAACA
GCCACAACGT
CTATATCATG
GCCGACAAGC
AGAAGAACGG
CATCAAGGTG
AACTTCAAGA
TCCGCCACAA
CATCGAGGAC
GGCAGCGTGC
AGCTCGCCGA
CCACTACCAG
CAGAACACCC
CCATCGGCGA
CGGCCCCGTG
CTGCTGCCCG
ACAACCACTA
CCTGAGCACC
CAGTCCGCCC
TGAGCAAAGA
CCCCAACGAG
AAGCGCGATC
ACATGGTCCT
GCTGGAGTTC
GTGACCGCCG
CCGGGATCAC
TCTCGGCATG
GACGAGCTGT
ACAAGTCCGG
ACTCAGATCT
CGAGATCCAC
CGGATCTAGA
TAACTGATCA
TAATCAGCCA
TACCACATTT
GTAGAGGTTT
TACTTGCTTT
AAAAAACCTC
CCACACCTCC
CCCTGAACCT
GAAACATAAA
ATGAATGCAA
TTGTTGTTGT
TAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTAACGCGTT
GACATTGATT
ATTGACTAGT
TATTAATAGT
AATCAATTAC
GGGGTCATTA
GTTCATAGCC
CATATATGGA
GTTCCGCGTT
ACATAACTTA
CGGTAAATGG
CCCGCCTGGC
TGACCGCCCA
ACGACCCCCG
CCCATTGACG
TCAATAATGA
CGTATGTTCC
CATAGTAACG
CCAATAGGGA
CTTTCCATTG
ACGTCAATGG
GTGGAGTATT
TACGGTAAAC
TGCCCACTTG
GCAGTACATC
AAGTGTATCA
TATGCCAAGT
ACGCCCCCTA
TTGACGTCAA
TGACGGTAAA
TGGCCCGCCT
GGCATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTCT
ATATAAGCAG
AGCTCTCTGG
CTAACTAGAG
AACCCACTGC
TTACTGGCTT
ATCGAAATTA
ATACGACTCA
CTATAGGGAG
ACCCAAGCTG
GCTAGCGTTT
AAACGGGCCC
TCTAGACTCG
AGCGGCCGCC
ACTGTGCTGG
ATATCGCCAC
CATGGCAAGA
CAAGGCTGTT
TCGGGTCATA
CCAGGTAATA
TCCTTGTTCA
CTTTTGCCAT
CGGCGTCAAT
CTCTGCTTAG
GATTCACAGC
AAGTCGAATT
AAGAGGGCCG
AATGGGATGA
AGGACCTCCC
ACAGTGTTAT
CTGACTCTCC
ATGGACCAAC
ACATCTGGAT
CCTGCAAAGG
TAGATGCTTT
GAGCTTCAAG
AGGTTGGACC
TCCTGACTGT
CGGTGTGACA
ACCTATGTAA
GAGCTACAGC
AGCTGCTGCC
ATGATTTTGA
TGAGCTCTGT
TTGAAAACAG
CTCGAGGCTG
GGAGTGCACC
AAAGACAGAT
GTGGGGAAGT
ACGAAATGAG
GAAAATGCCT
GTCACTGCTC
AGAAGACTGC
TTGTCCCGGG
GAGACTGCTG
TACCAACTAC
CAAGTGGTCT
GCAAAGGAGA
ATCACACTGG
GTAGATGATG
ACTGTGAAGA
AATAAGAGTC
CCCGAATGCC
CTGCAGGGTT
TGTCCGCCCT
CCGTTAATCA
TCTTCTCTGT
GGATGGATTC
CGTGCATCGT
ACATGAAGAA
AGGCAGCAAG
GTTATGCCCA
ACATTGAGAA
ACTGCGGTCC
TGTGGCACCC
ATGCTCCCTA
CATGAGGCCT
GTGTACCCTA
CAAAAACCTT
CCCTAATCTG
TATACGCTGG
CCACTGGTTT
ATATCCAGAA
TCCCATGGAA
TCGTTGGCAA
TTCAATGTAT
GACCCTGTCT
TTGATGCTAC
TTTCCATCTT
CGAGGGCGAG
AGAAGTTTAA
CCATAGATGG
TGGGGAGGCC
AACCGCTATG
GATTACAGCC
ACCAAGCAAG
GGGTGAGAGC
CGGGACATTC
TTTTGGTCTG
TGAGCATCCC
TCACGAGCGG
AGAATCCTAA
CTATCCTTCA
GTGGCTTTCC
CTGCCAGACA
ATGAGAGGCC
TTCAGTTTAT
GCCTTCTACT
CCGAGCAGCC
TGATTTTTCT
GGACACAAGT
ACGGCCCTTT
TGGCCCTGAG
GAGAGTAGTT
ATGGCTCACC
TCTTACTCCG
GCTAAAAGAC
CTAAGAGGAA
AGTTGCCCCT
AAGAGGAGAC
AGGAAAGACC
AGTTGCTCCT
CCAAAGAAAA
GAAGAAGAAA
ATTACATAGG
ATGGATCATT
ATACAGCGGA
AACACGTCAG
GACAAAATGA
CAAATCCTCT
GAGGGAGATT
GACAAGACCG
TGGGGCAGTT
AATGGACGGA
CTGAAACAAC
TCAAGCTGCA
CCGTTGTGTG
AATGTTATCT
TTGTTGGAGA
CCATGGAATG
GAAGACGTGA
CATGTGACAG
AACTGAGTTC
TTGAGCAACT
ATCTGACTAA
CGTGGATGAT
ATTACTTTAG
TACCTGGAAC
TCTAGGAAGA
ATTCGACCCA
AGATTCCCAA
TAATCTTAAA
TATGACCCTA
AAGCCATTAT
TGCTAACCTC
ACGTGTAAAA
AACCAGATCA
GCACTTTAAG
CCTTACATGA
AACAGCACCT
TCCCAAACGT
TTGCACTATG
CCAACAATCG
GAGAATCGAG
GATCTCCATT
TATTGGTGGA
ACGCAGATGG
CATGTTGCAA
GGAAACCTTT
GGACGTTTAT
AAGAAGCCGT
CAGGAAAATG
TTTTTTCCAG
GGTGACCACG
GCTTTGATAA
CAAGGTCAAT
AGCATGCAGA
CTGTTTTTGT
AGGTTATGGC
CCAACTTTTA
AGTACAGGAC
TAAAGTGCCT
CCATTTGAAA
ACATTGAACT
TTATAATGTT
ATGTGCGATC
TCCTAGGCTT
GAAGCCAGCT
CCCAATAATG
GAACACATGG
AAGTTTGAAT
CACCTGCTAC
GCACAAATAC
CTTTAGGCCA
ACCCTACCAG
AGGAAGTCAG
CAGACCCAAT
TACCCAGGGA
TTATGTACCT
TCAGTCTGAT
TTTGACCTGG
GCTGCACCTG
TGATGATAAG
GTAGAGCCAA
AGAACAAATT
GGAAGAACTA
AATAAACGCC
TTCATACCAA
AGGATCTACA
GAAGAGAGAC
ATCTCCTGTA
TGGACGACCT
GCAGTGCTTT
ATCGGACTAG
CTATGATATC
TTATACCATA
CGGACTTTGA
AAGTGGTTAC
AGTGAAATAT
TCTTAATGCC
TCTCTGGACT
TCTTATACCA
TTTCTAAGCA
GGCTGAGGTC
TCTAGCATCC
CAGAGCACCT
GACCAACTGT
GTTCGCCCTG
ATGTCCGTGT
ATCTCCTGGA
TTCAGTCAGA
ACTGTTTAGC
CTATAAAAAT
GATAAACAGA
TGTCCTATGG
ATTCCTTTTT
CCTCCCTATC
TGAGCTCTTC
CCCAGAAGCG
AAATATGATG
CATTCCTTGT
AACCAACATG
GTTCCAATGT
ACCCTGCCTT
CAAACGTGTT
TGGACTTATT
TCCAAAGGGT
CTTGGTGAAG
AAATATGCGT
CAGAAAGGAA
TGGGGTCAAC
GTAATAAGTG
GACCGATCTT
TGACTACAAT
TACAATGGCT
TACGTGACAT
TGAGGATGAA
ATTAAACAGT
ATGTGGAAGG
CAGCTCTATT
CCTGTCCCTA
CCCACTACTA
CAGCATCATC
ACCAGCTGCC
TGGACTTCAC
TCAGCCTGCA
GACAAGTGTG
ATGGTCCTCT
CTCTGTGTCT
TCTTTCATCC
TTCCTCACCG
ACCTGACAAT
GATGAGAGCT
GTAATAGTTC
CGAGGATGAG
TCGAAGTGGG
TAGAGGAACT
CATGAAGATG
CACACAGCTC
GGGTGAGGGA
CATCGAGCAT
CTCACCGGTT
TGGATTTCTA
CCGGAAGACT
AGCCGTAGCT
ATTCGGAAAT
TCTGACCCTC
AAGACATACC
TGCATACATA
TGAGAGCGAG
ATTGGATCCG
ACTACAAAGA
CCATGACGGT
GATTATAAAG
ATCATGACAT
CGACTACAAG
GATGACGATG
ACAAGTAGAA
GCTTAAGTTT
AAACCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGACAA
TAGCAGGCAT
GCTGGGGATG
CGGTGGGCTC
TATGGGTCGA
CTTCGAGCAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGG
ATCGTCTAGC
ATCGAAGATC
CAATAACTTC
GTATAGCATA
CATTATACGA
AGTTATAAGT
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCAATGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
GACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAACACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTCTAAGA
AACCATTATT
ATCATGACAT
TAACCTATAA
AAATAGGCGT
ATCACTCTAG
GCAAAATAGC
ACCCTCCCGC
TCCAGAACAA
CATACAGCGC
TTCACAGCGG
CAGCCTAACA
GTCAGCCTTA
CCAGTAAAAA
AGAAAACCTA
TTAAAAAAAC
ACCACTCGAC
ACGGCACCAG
CTCAATCAGT
CACAGTGTAA
AAAAGGGCCA
AGTGCAGAGC
GAGTATATAT
AGGACTAAAA
AATGACGTAA
CGGTTAAAGT
CCACAAAAAA
CACCCAGAAA
ACCGCACGCG
AACCTACGCC
CAGAAACGAA
AGCCAAAAAA
CCCACAACTT
CCTCAAATCG
TCACTTCCGT
TTTCCCACGT
TACGTAACTT
CCCATTTTAA
GAAAACTACA
ATTCCCAACA
CATACAAGTT
ACTCCGCCCT
AAAACCTACG
TCACCCGCCC
CGTTCCCACG
CCCCGCGCCA
CGTCACAAAC
TCCACCCCCT
CATTATCATA
TTGGCTTCAA
TCCAAAATAA
GGTATA