名称: | pLV3-CMV-3×Myc-TBC1D4(human)-Puro |
---|---|
别称: | NM_014832.5;AS160; NIDDM5 |
基因长: | 3897bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-3×Myc-MCS-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-3×Myc-TBC1D4(human)-Puro适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动人源TBC1D4基因转录,促进TBC1D4基因N端融合表达3×Myc标签,便于抗-Myc抗体在免疫印迹、免疫沉淀或免疫荧光等实验中识别和追踪带有Myc标签的目的蛋白,从而研究目的蛋白的表达水平、亚细胞定位以及与其他蛋白的相互作用等。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCTAGCG
AATTCGCCAC
CATGGAACAA
AAACTAATAT
CAGAGGAAGA
TTTGGAACAG
AAACTAATCA
GTGAAGAAGA
TTTAGAGCAA
AAGTTAATTT
CCGAGGAGGA
CTTACTCGAG
ATGGAGCCGC
CCAGCTGCAT
TCAGGATGAG
CCGTTCCCGC
ACCCCCTGGA
GCCCGAGCCG
GGCGTCTCAG
CTCAGCCCGG
CCCCGGGAAG
CCAAGCGATA
AGCGGTTCCG
GCTGTGGTAC
GTTGGGGGGT
CGTGCCTGGA
CCACAGGACC
ACGCTGCCTA
TGCTGCCCTG
GCTCATGGCC
GAGATCCGCA
GGCGCAGCCA
GAAGCCCGAG
GCGGGCGGCT
GCGGGGCGCC
GGCGGCCCGA
GAGGTGATCC
TGGTGCTCAG
CGCGCCCTTC
CTGCGTTGCG
TCCCCGCGCC
GGGCGCTGGG
GCCTCGGGGG
GCACTAGTCC
GTCGGCCACG
CAGCCCAACC
CGGCGGTATT
CATCTTCGAG
CACAAGGCGC
AGCATATCTC
GCGCTTCATC
CACAACAGCC
ACGACCTCAC
CTACTTTGCC
TACCTGATCA
AGGCGCAGCC
CGACGACCCC
GAGTCGCAGA
TGGCCTGCCA
CGTTTTCCGC
GCCACAGACC
CCAGCCAGGT
TCCTGATGTT
ATTAGCAGCA
TAAGGCAATT
ATCTAAAGCG
GCCATGAAAG
AGGATGCCAA
ACCCAGCAAA
GATAATGAGG
ACGCCTTTTA
CAACTCTCAG
AAGTTCGAAG
TCCTGTACTG
TGGAAAGGTG
ACCGTGACCC
ACAAGAAGGC
CCCCTCAAGC
CTCATCGATG
ACTGCATGGA
GAAGTTCAGC
CTGCACGAAC
AGCAGCGCCT
GAAGATCCAA
GGGGAGCAGC
GCGGTCCGGA
CCCAGGAGAG
GACCTGGCTG
ACTTGGAGGT
GGTGGTGCCC
GGGTCCCCCG
GAGACTGCCT
GCCGGAGGAG
GCTGACGGCA
CCGACACCCA
CCTTGGCTTA
CCTGCCGGGG
CCAGCCAGCC
TGCCCTGACC
AGCTCTCGGG
TCTGCTTCCC
TGAGCGGATT
TTGGAAGATT
CTGGCTTTGA
TGAGCAGCAG
GAGTTTCGGT
CTCGGTGCAG
CAGTGTCACC
GGCGTGCAAC
GGAGAGTTCA
CGAGGGCAGC
CAGAAATCCC
AGCCGCGACG
GAGACACGCG
AGCGCACCCA
GTCACGTCCA
GCCCTCGGAC
TCGGAGAAGA
ACAGGACCAT
GCTCTTCCAG
GTTGGGCGAT
TTGAGATTAA
CCTTATCAGT
CCAGACACTA
AATCAGTTGT
GCTAGAAAAG
AATTTTAAAG
ATATCTCCTC
TTGTTCTCAG
GGTATAAAGC
ATGTGGATCA
CTTTGGCTTT
ATCTGCCGGG
AGTCTCCAGA
GCCTGGACTT
AGCCAGTATA
TTTGTTATGT
ATTCCAGTGT
GCCAGCGAAT
CTCTGGTTGA
TGAGGTAATG
CTGACTCTGA
AACAGGCCTT
CAGTACGGCG
GCTGCCCTGC
AGAGTGCCAA
GACGCAGATT
AAACTGTGTG
AGGCCTGCCC
GATGCACTCT
TTGCATAAGC
TCTGTGAAAG
GATTGAAGGT
CTCTACCCAC
CAAGAGCCAA
GCTGGTGATA
CAGAGGCATC
TCTCATCACT
GACAGATAAT
GAGCAAGCTG
ACATCTTTGA
AAGAGTTCAG
AAAATGAAGC
CAGTCAGTGA
CCAGGAAGAA
AATGAACTTG
TGATTTTACA
CCTGAGGCAG
CTGTGTGAAG
CCAAGCAGAA
GACACACGTG
CACATCGGGG
AAGGCCCTTC
TACTATTTCA
AATAGTACAA
TCCCAGAAAA
TGCAACAAGC
AGTGGAAGGT
TCAAACTTGA
CATTCTGAAA
AATAAAGCTA
AGAGATCCTT
AACTAGCTCC
CTGGAAAATA
TCTTCTCAAG
GGGAGCTAAC
AGAATGAGAG
GTCGGCTTGG
AAGTGTGGAC
AGTTTTGAAC
GGTCCAACAG
TCTTGCTTCA
GAGAAGGACT
ACTCACCAGG
GGATTCTCCA
CCAGGGACAC
CGCCAGCGTC
CCCACCGTCC
TCAGCTTGGC
AAACGTTTCC
CGAAGAGGAT
TCCGACTCCC
CGCAGTTTCG
AAGACGGGCA
CACACGTTCA
GCCACCCACC
TTCAAGCACA
AAGAGAAAGC
TGAATTTGCA
GGATGGGAGG
GCTCAGGGTG
TGCGTTCCCC
TCTGCTGAGG
CAGAGCTCCA
GTGAACAGTG
CAGCAATCTT
TCGTCAGTTC
GACGCATGTA
CAAGGAGAGT
AATTCTTCCT
CCAGTCTTCC
AAGTCTTCAC
ACTTCCTTCT
CTGCCCCTTC
CTTCACTGCC
CCCTCTTTCC
TGAAAAGCTT
TTACCAGAAT
TCAGGTAGAC
TGTCCCCACA
GTATGAAAAT
GAAATCAGAC
AAGACACTGC
TTCAGAATCA
AGTGATGGAG
AAGGGAGAAA
AAGGACCTCA
TCTACCTGCA
GCAATGAGTC
CCTAAGTGTG
GGAGGAACCT
CTGTCACTCC
TCGCCGGATC
TCCTGGCGGC
AGCGCATTTT
CCTCAGGGTT
GCTTCTCCCA
TGAACAAATC
TCCCTCAGCA
ATGCAACAGC
AAGATGGATT
GGACAGGAAC
GAGCTGCTGC
CACTGTCCCC
CCTCTCTCCA
ACCATGGAGG
AGGAACCGCT
GGTTGTATTC
CTGTCTGGGG
AGGATGACCC
AGAAAAGATT
GAAGAAAGAA
AGAAATCAAA
AGAACTGAGG
AGCTTGTGGA
GAAAAGCTAT
ACACCAACAA
ATCTTGTTAC
TTCGAATGGA
AAAAGAAAAC
CAGAAACTTG
AAGCAAGCAG
AGATGAACTC
CAGTCCAGAA
AAGTTAAATT
AGACTATGAA
GAAGTTGGTG
CATGTCAGAA
AGAGGTCTTA
ATAACTTGGG
ATAAGAAGTT
GTTAAACTGC
AGAGCTAAAA
TCAGATGTGA
TATGGAAGAT
ATTCATACTC
TTCTTAAAGA
AGGAGTTCCC
AAAAGTCGAC
GAGGAGAAAT
TTGGCAGTTT
CTGGCTTTAC
AGTACCGACT
CAGACACAGA
TTGCCTAATA
AACAACAGCC
TCCTGACATA
TCCTATAAGG
AACTTTTGAA
GCAGCTCACT
GCTCAGCAGC
ATGCGATTCT
CGTGGATTTA
GGAAGGACGT
TTCCTACTCA
CCCTTACTTT
TCAGTACAGC
TTGGGCCAGG
ACAGCTGTCA
CTGTTTAACC
TCCTGAAAGC
CTATTCTTTG
CTGGACAAAG
AAGTGGGATA
CTGTCAGGGG
ATCAGCTTTG
TGGCTGGAGT
CCTGCTTCTG
CACATGAGTG
AAGAGCAAGC
CTTTGAAATG
CTGAAATTCC
TCATGTATGA
CCTCGGCTTC
CGCAAGCAGT
ACAGACCTGA
CATGATGTCG
CTGCAGATTC
AAATGTACCA
GCTGTCCAGG
CTCCTTCATG
ACTATCACAG
AGATCTCTAC
AATCACCTTG
AAGAAAATGA
AATCAGCCCC
AGTCTTTATG
CTGCCCCCTG
GTTCCTCACA
TTGTTTGCCT
CTCAGTTTTC
ATTAGGATTT
GTAGCCAGAG
TTTTTGATAT
TATTTTTCTT
CAGGGAACTG
AAGTTATATT
CAAGGTTGCA
CTCAGCCTAC
TGAGCAGCCA
AGAGACACTT
ATAATGGAAT
GTGAGAGCTT
TGAAAATATT
GTTGAGTTTC
TTAAAAACAC
GCTACCTGAT
ATGAATACCT
CTGAAATGGA
AAAAATTATT
ACCCAGGTTT
TTGAGATGGA
TATTTCTAAG
CAGTTGCATG
CCTATGAGGT
GGAATATCAT
GTGCTACAGG
ATGAGCTTCA
GGAATCTTCA
TATTCCTGTG
AGGATAGTGA
AACTTTGGAG
AAGCTGGAGA
GGGCCAATAG
CCAACTGAAA
AGACAAAACA
TGGACCTCCT
AGAAAAATTA
CAGGTAGCTC
ATACTAAAAT
CCAGGCCTTG
GAATCAAACC
TGGAAAATCT
TTTGACGAGA
GAGACCAAAA
TGAAGTCTTT
AATCCGGACC
CTGGAACAAG
AAAAAATGGC
TTATCAAAAG
ACAGTGGAGC
AACTCCGGAA
GCTGCTGCCC
GCGGATGCTC
TAGTCAATTG
TGACCTGTTG
CTGAGAGACC
TAAACTGCAA
CCCTAACAAC
AAAGCCAAGA
TAGGAAATAA
GCCATAAGGA
TCCGATATCT
AGCGGCCGCG
AAGGATCTGC
GATCGCTCCG
GTGCCCGTCA
GTGGGCAGAG
CGCACATCGC
CCACAGTCCC
CGAGAAGTTG
GGGGGAGGGG
TCGGCAATTG
AACGGGTGCC
TAGAGAAGGT
GGCGCGGGGT
AAACTGGGAA
AGTGATGTCG
TGTACTGGCT
CCGCCTTTTT
CCCGAGGGTG
GGGGAGAACC
GTATATAAGT
GCAGTAGTCG
CCGTGAACGT
TCTTTTTCGC
AACGGGTTTG
CCGCCAGAAC
ACAGCTGAAG
CTTCGAGGGG
CTCGCATCTC
TCCTTCACGC
GCCCGCCGCC
CTACCTGAGG
CCGCCATCCA
CGCCGGTTGA
GTCGCGTTCT
GCCGCCTCCC
GCCTGTGGTG
CCTCCTGAAC
TGCGTCCGCC
GTCTAGGTAA
GTTTAAAGCT
CAGGTCGAGA
CCGGGCCTTT
GTCCGGCGCT
CCCTTGGAGC
CTACCTAGAC
TCAGCCGGCT
CTCCACGCTT
TGCCTGACCC
TGCTTGCTCA
ACTCTACGTC
TTTGTTTCGT
TTTCTGTTCT
GCGCCGTTAC
AGATCCAAGC
TGTGACCGGC
GCCTACGCTA
GATGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCCGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGACCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGCGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
GTGCCCGAAG
GACCGCGCAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
AGTCGACAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAATCATCGT
CCTTTCCTTG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCCTGG
TACCTTTAAG
ACCAATGACT
TACAAGGCAG
CTGTAGATCT
TAGCCACTTT
TTAAAAGAAA
AGGGGGGACT
GGAAGGGCTA
ATTCACTCCC
AACGAAAATA
AGATCTGCTT
TTTGCTTGTA
CTGGGTCTCT
CTGGTTAGAC
CAGATCTGAG
CCTGGGAGCT
CTCTGGCTAA
CTAGGGAACC
CACTGCTTAA
GCCTCAATAA
AGCTTGCCTT
GAGTGCTTCA
AGTAGTGTGT
GCCCGTCTGT
TGTGTGACTC
TGGTAACTAG
AGATCCCTCA
GACCCTTTTA
GTCAGTGTGG
AAAATCTCTA
GCAGTAGTAG
TTCATGTCAT
CTTATTATTC
AGTATTTATA
ACTTGCAAAG
AAATGAATAT
CAGAGAGTGA
GAGGAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGCTCT
AGCTATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGACTTTTG
CAGAGACGGC
CCAAATTCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
GACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTCTAAGA
AACCATTATT
ATCATGACAT
TAACCTATAA
AAATAGGCGT
ATCACGAGGC
CCTTTCGTCT
CGCGCGTTTC
GGTGATGACG
GTGAAAACCT
CTGACACATG
CAGCTCCCGG
AGACGGTCAC
AGCTTGTCTG
TAAGCGGATG
CCGGGAGCAG
ACAAGCCCGT
CAGGGCGCGT
CAGCGGGTGT
TGGCGGGTGT
CGGGGCTGGC
TTAACTATGC
GGCATCAGAG
CAGATTGTAC
TGAGAGTGCA
CCATATGCGG
TGTGAAATAC
CGCACAGATG
CGTAAGGAGA
AAATACCGCA
TCAGGCGCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGCCAAG
CTG