名称: | pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4(mouse) |
---|---|
别称: | NM_025663.4;2610029K21Rik; Gpatc4 |
基因长: | 1248bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | Fluc |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pT3-EF1A-MYC-IRES-luc |
正向引物: | IRES-F1 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4(mouse)是适用于哺乳细胞过表达的质粒。质粒带有的EF1a强启动子可启动小鼠源源Gpatch4基因转录,促进Gpatch4基因N端融合表达Myc和Flag标签,便于抗体在免疫印迹、免疫沉淀或免疫荧光等实验中识别和追踪带有标签的目的蛋白,从而研究目的蛋白的表达水平、亚细胞定位以及与其他蛋白的相互作用等。。质粒携带Puro抗性基因,用于筛选出表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CTAGCTCGAG
ATCTTAATTA
AACTAGTATC
GATATAACTT
CGTATAATGT
ATGCTATACG
AAGTTATATG
TACGGGCCAG
ATATACGCGT
TGACATTGAT
TATTGACTAG
GCTTTTGCAA
AAAGCTTTGC
AAAGATGGAT
AAAGTTTTAA
ACAGAGAGGA
ATCTTTGCAG
CTAATGGACC
TTCTAGGTCT
TGAAAGGAGT
GGGAATTGGC
TCCGGTGCCC
GTCAGTGGGC
AGAGCGCACA
TCGCCCACAG
TCCCCGAGAA
GTTGGGGGGA
GGGGTCGGCA
ATTGAACCGG
TGCCTAGAGA
AGGTGGCGCG
GGGTAAACTG
GGAAAGTGAT
GTCGTGTACT
GGCTCCGCCT
TTTTCCCGAG
GGTGGGGGAG
AACCGTATAT
AAGTGCAGTA
GTCGCCGTGA
ACGTTCTTTT
TCGCAACGGG
TTTGCCGCCA
GAACACAGGT
AAGTGCCGTG
TGTGGTTCCC
GCGGGCCTGG
CCTCTTTACG
GGTTATGGCC
CTTGCGTGCC
TTGAATTACT
TCCACCTGGC
TGCAGTACGT
GATTCTTGAT
CCCGAGCTTC
GGGTTGGAAG
TGGGTGGGAG
AGTTCGAGGC
CTTGCGCTTA
AGGAGCCCCT
TCGCCTCGTG
CTTGAGTTGA
GGCCTGGCCT
GGGCGCTGGG
GCCGCCGCGT
GCGAATCTGG
TGGCACCTTC
GCGCCTGTCT
CGCTGCTTTC
GATAAGTCTC
TAGCCATTTA
AAATTTTTGA
TGACCTGCTG
CGACGCTTTT
TTTCTGGCAA
GATAGTCTTG
TAAATGCGGG
CCAAGATCTG
CACACTGGTA
TTTCGGTTTT
TGGGGCCGCG
GGCGGCGACG
GGGCCCGTGC
GTCCCAGCGC
ACATGTTCGG
CGAGGCGGGG
CCTGCGAGCG
CGGCCACCGA
GAATCGGACG
GGGGTAGTCT
CAAGCTGGCC
GGCCTGCTCT
GGTGCCTGGC
CTCGCGCCGC
CGTGTATCGC
CCCGCCCTGG
GCGGCAAGGC
TGGCCCGGTC
GGCACCAGTT
GCGTGAGCGG
AAAGATGGCC
GCTTCCCGGC
CCTGCTGCAG
GGAGCTCAAA
ATGGAGGACG
CGGCGCTCGG
GAGAGCGGGC
GGGTGAGTCA
CCCACACAAA
GGAAAAGGGC
CTTTCCGTCC
TCAGCCGTCG
CTTCATGTGA
CTCCACGGAG
TACCGGGCGC
CGTCCAGGCA
CCTCGATTAG
TTCTCGAGCT
TTTGGAGTAC
GTCGTCTTTA
GGTTGGGGGG
AGGGGTTTTA
TGCGATGGAG
TTTCCCCACA
CTGAGTGGGT
GGAGACTGAA
GTTAGGCCAG
CTTGGCACTT
GATGTAATTC
TCCTTGGAAT
TTGCCCTTTT
TGAGTTTGGA
TCTTGGTTCA
TTCTCAAGCC
TCAGACAGTG
GTTCAAAGTT
TTTTTCTTCC
ATTTCAGGTG
TCGTGAGGAA
TTAGCTTGGT
ACTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGG
TAAGCTTGGT
ACCGAGCTCG
GATCCACTAG
TCCAGTGTGG
TGGAATTCTG
CAGATATCAA
CAAGTTTGTA
CAAAAAAGCA
GGCTTCGCCA
CCATGCCCCT
CAACGTTAGC
TTCACCAACA
GGAACTATGA
CCTCGACTAC
GACTCGGTGC
AGCCGTATTT
CTACTGCGAC
GAGGAGGAGA
ACTTCTACCA
GCAGCAGCAG
CAGAGCGAGC
TGCAGCCCCC
GGCGCCCAGC
GAGGATATCT
GGAAGAAATT
CGAGCTGCTG
CCCACCCCGC
CCCTGTCCCC
TAGCCGCCGC
TCCGGGCTCT
GCTCGCCCTC
CTACGTTGCG
GTCACACCCT
TCTCCCTTCG
GGGAGACAAC
GACGGCGGTG
GCGGGAGCTT
CTCCACGGCC
GACCAGCTGG
AGATGGTGAC
CGAGCTGCTG
GGAGGAGACA
TGGTGAACCA
GAGTTTCATC
TGCGACCCGG
ACGACGAGAC
CTTCATCAAA
AACATCATCA
TCCAGGACTG
TATGTGGAGC
GGCTTCTCGG
CCGCCGCCAA
GCTCGTCTCA
GAGAAGCTGG
CCTCCTACCA
GGCTGCGCGC
AAAGACAGCG
GCAGCCCGAA
CCCCGCCCGC
GGCCACAGCG
TCTGCTCCAC
CTCCAGCTTG
TACCTGCAGG
ATCTGAGCGC
CGCCGCCTCA
GAGTGCATCG
ACCCCTCGGT
GGTCTTCCCC
TACCCTCTCA
ACGACAGCAG
CTCGCCCAAG
TCCTGCGCCT
CGCAAGACTC
CAGCGCCTTC
TCTCCGTCCT
CGGATTCTCT
GCTCTCCTCG
ACGGAGTCCT
CCCCGCAGGG
CAGCCCCGAG
CCCCTGGTGC
TCCATGAGGA
GACACCGCCC
ACCACCAGCA
GCGACTCTGA
GGAGGAACAA
GAAGATGAGG
AAGAAATCGA
TGTTGTTTCT
GTGGAAAAGA
GGCAGGCTCC
TGGCAAAAGG
TCAGAGTCTG
GATCACCTTC
TGCTGGAGGC
CACAGCAAAC
CTCCTCACAG
CCCACTGGTC
CTCAAGAGGT
GCCACGTCTC
CACACATCAG
CACAACTACG
CAGCGCCTCC
CTCCACTCGG
AAGGACTATC
CTGCTGCCAA
GAGGGTCAAG
TTGGACAGTG
TCAGAGTCCT
GAGACAGATC
AGCAACAACC
GAAAATGCAC
CAGCCCCAGG
TCCTCGGACA
CCGAGGAGAA
TGTCAAGAGG
CGAACACACA
ACGTCTTGGA
GCGCCAGAGG
AGGAACGAGC
TAAAACGGAG
CTTTTTTGCC
CTGCGTGACC
AGATCCCGGA
GTTGGAAAAC
AATGAAAAGG
CCCCCAAGGT
AGTTATCCTT
AAAAAAGCCA
CAGCATACAT
CCTGTCCGTC
CAAGCAGAGG
AGCAAAAGCT
CATTTCTGAA
GAGGACTTGT
TGCGGAAACG
ACGAGAACAG
TTGAAACACA
AACTTGAACA
GCTACGGAAC
TCTTGTGCGG
ACCCAGCTTT
CTTGTACAAA
GTGGTTGATA
TCCAGCACAG
TGGCGGCCGC
TCGAGTCTAG
AGGGCCCGCG
GTTCGAAGGT
AAGCCTATCC
CTAACCCTCT
CCTCGGTCTC
GATTCTACGC
GTACCGGTTA
GTAATGAGTT
TCCCCCCCCC
CCTAACGTTA
CTGGCCGAAG
CCGCTTGGAA
TAAGGCCGGT
GTGCGTTTGT
CTATATGTTA
TTTTCCACCA
TATTGCCGTC
TTTTGGCAAT
GTGAGGGCCC
GGAAACCTGG
CCCTGTCTTC
TTGACGAGCA
TTCCTAGGGG
TCTTTCCCCT
CTCGCCAAAG
GAATGCAAGG
TCTGTTGAAT
GTCGTGAAGG
AAGCAGTTCC
TCTGGAAGCT
TCTTGAAGAC
AAACAACGTC
TGTAGCGACC
CTTTGCAGGC
AGCGGAACCC
CCCACCTGGC
GACAGGTGCC
TCTGCGGCCA
AAAGCCACGT
GTATAAGATA
CACCTGCAAA
GGCGGCACAA
CCCCAGTGCC
ACGTTGTGAG
TTGGATAGTT
GTGGAAAGAG
TCAAATGGCT
CTCCTCAAGC
GTATTCAACA
AGGGGCTGAA
GGATGCCCAG
AAGGTACCCC
ATTGTATGGG
ATCTGATCTG
GGGCCTCGGT
GCACATGCTT
TACATGTGTT
TAGTCGAGGT
TAAAAAAACG
TCTAGGCCCC
CCGAACCACG
GGGACGTGGT
TTTCCTTTGA
AAAACACGAT
GATAATATGG
CCACAACCGC
CACCATGGAC
TACAAAGACC
ATGACGGTGA
TTATAAAGAT
CATGACATCG
ACTACAAGGA
TGACGATGAC
AAGATGAGTG
TCACCCCAGA
GGTCAAGAGC
CGTGGGATGA
AGTTTGCTGA
AGAGCAGCTG
CTGAAGCATG
GATGGACTCA
AGGCAAGGGC
CTGGGCCGGA
GGGAGAATGG
CATTACCCAG
GCCCTCAAGG
TGACACTGAA
GCAAGATAAC
CATGGGGTGG
GACATGACCC
TGCCAAGGAA
TTTACAGACC
ACTGGTGGAG
TGATCTCTTC
AACAAGACTG
CCGCCAGCTT
GGTAGTGGAC
TCGGGGAAGG
ATGGAGTGCA
GATAAGACGT
CTTTCCAAGG
AGACCACCCA
GCGCAGTCAC
CCCAAACCCA
GCTTGCTGTA
TCAGAAGTTT
GTGAAGACAG
CCACACTAAC
CTCGGGTGAG
GAGAAGCCTG
ACAGAGACTT
GGGGAACTGC
AGTGATGTTG
ACAACCATGA
GCCCACGCCC
CCAAAGATTC
TGACTGATGA
GATGCTACTC
AAGGCTTGCG
AGGGGCGAAC
AGCACACAAG
GCTGCCCGGA
TTGGTATCAC
CATGAAGGCC
AAGCTTGCTC
GCCTGGAGGC
CCAAGAGCAG
GCCTTTCTGG
CTCAGCTCAA
AGGCTCAAAG
GCTCTGGGCA
CCTCTCAACC
ACTGACTGAC
AGTGAGCCTT
CCCAAAAAAA
GAAAAAGAAG
AGGAAGCAGA
AAGAGGGGGA
AGAGGCTGCA
ACAACTGAAA
AGAGTCTAGG
TGACGAGCTG
CTGGGACACA
CGGACAGGAG
CTTCAGGGAC
AGCAGGAAGA
AGAAGAAGAG
GCAGAAAGCC
GAGAGACAGG
GAACGGCTAT
AGGAAGTGAG
GAGGAAGAGG
CTGCAGGAGA
GAGTGGGCCC
AGAGAACTGA
GCACAGAGCA
ATCTGATCAG
CCTTCCAGGA
AAAAGAAGAA
AAGGAGGAAG
CAGCGCCATG
AAGAAGACGG
GGAGATGGGA
GTCTGTGATG
AAGGAGGTAG
AGATGTGACA
AGCCGGCCAA
AGGCAGTCAA
CAGTGGAGGA
GACAAAGACC
CCAGGAGAAG
CAGCAAGAAG
CGAGGGACGT
GTGGGGAGGA
CTTAGATACC
CAAGAGGAAG
AAGGGAAGGA
TGACCTAACA
AAAGGAGAGA
GAAAGGTCAG
AAGAAAAGAC
AAAAGGAAAA
GACAGCAGTG
CTGTGAGGAG
GACTTAGATG
TAAGCAGTAA
AGATGATGGT
GGGACATGGG
TAGCAGAGGA
TGCAGGTGAG
AGAAGTCGGC
AATACCCAAA
GGAGAGAGCC
AAAAAGAAGA
AGCGGAAGAG
GGACCGAGGG
TCTGAAGTGG
ATCTCAGCTG
ATTCTAGGAT
CCGTCGACCT
GCAGCCAAGC
TTATCGATAA
ACCCGCTGAT
CAGCCTCGAC
TGTGCCTTCT
AGTTGCCAGC
CATCTGTTGT
TTGCCCCTCC
CCCGTGCCTT
CCTTGACCCT
GGAAGGTGCC
ACTCCCACTG
TCCTTTCCTA
ATAAAATGAG
GAAATTGCAT
CGCATTGTCT
GAGTAGGTGT
CATTCTATTC
TGGGGGGTGG
GGTGGGGCAG
GACAGCAAGG
GGGAGGATTG
GGAAGACAAT
AGCAGGCATG
CTGGGGATGC
GGTGGGCTCT
ATGGCTTCTG
AGGCGGAAAG
AACCAGCTGG
GGCTCTAGGG
GGTATCCCCA
CGCGCCCTGT
AGCGGCGCAT
TAAATAACTT
CGTATAATGT
ATGCTATACG
AAGTTATATC
GTATGCATGC
TTTGCATACT
TCTGCCTGCT
GGGGAGCCTG
GGGACTTTCC
ACACCCTAAC
TGACACACAT
TCCACAGAAT
TCCTTGAAAT
ACATCCACAG
GTACACCTCC
AATTGACTCA
AATGATGTCA
ATTAGTCTAT
CAGAAGCTTC
TAAAGCCATG
ACATCATTTT
CTGGAATTTT
CCAAGCTGTT
TAAAGGCACA
GTCAACTTAG
TGTATGTAAA
CTTCTGACCC
ACTGGAATTG
TGATACAGTG
AATTATAAGT
GAAATAATCT
GTCTGTAAAC
AATTGTTGGA
AAAATGACTT
GTGTCATGCA
CAAAGTAGAT
GTCCTAACTG
ACTTGCCAAA
ACTATTGTTT
GTTAACAAGA
AATTTGTGGA
GTAGTTGAAA
AACGAGTTTT
AATGACTCCA
ACTTAAGTGT
ATGTAAACTT
CCGACTTCAA
CTGTAGGGTA
CCGAGCTCGA
ATTCACTGGC
CGTCGTTTTA
CAACGTCGTG
ACTGGGAAAA
CCCTGGCGTT
ACCCAACTTA
ATCGCCTTGC
AGCACATCCC
CCTTTCGCCA
GCTGGCGTAA
TAGCGAAGAG
GCCCGCACCG
ATCGCCCTTC
CCAACAGTTG
CGCAGCCTGA
ATGGCGAATG
GCGCCTGATG
CGGTATTTTC
TCCTTACGCA
TCTGTGCGGT
ATTTCACACC
GCATATGGTG
CACTCTCAGT
ACAATCTGCT
CTGATGCCGC
ATAGTTAAGC
CAGCCCCGAC
ACCCGCCAAC
ACCCGCTGAC
GCGCCCTGAC
GGGCTTGTCT
GCTCCCGGCA
TCCGCTTACA
GACAAGCTGT
GACCGTCTCC
GGGAGCTGCA
TGTGTCAGAG
GTTTTCACCG
TCATCACCGA
AACGCGCGAG
ACGAAAGGGC
CTCGTGATAC
GCCTATTTTT
ATAGGTTAAT
GTCATGATAA
TAATGGTTTC
TTAGACGTCA
GGTGGCACTT
TTCGGGGAAA
TGTGCGCGGA
ACCCCTATTT
GTTTATTTTT
CTAAATACAT
TCAAATATGT
ATCCGCTCAT
GAGACAATAA
CCCTGATAAA
TGCTTCAATA
ATATTGAAAA
AGGAAGAGTA
TGAGTATTCA
ACATTTCCGT
GTCGCCCTTA
TTCCCTTTTT
TGCGGCATTT
TGCCTTCCTG
TTTTTGCTCA
CCCAGAAACG
CTGGTGAAAG
TAAAAGATGC
TGAAGATCAG
TTGGGTGCAC
GAGTGGGTTA
CATCGAACTG
GATCTCAACA
GCGGTAAGAT
CCTTGAGAGT
TTTCGCCCCG
AAGAACGTTT
TCCAATGATG
AGCACTTTTA
AAGTTCTGCT
ATGTGGCGCG
GTATTATCCC
GTATTGACGC
CGGGCAAGAG
CAACTCGGTC
GCCGCATACA
CTATTCTCAG
AATGACTTGG
TTGAGTACTC
ACCAGTCACA
GAAAAGCATC
TTACGGATGG
CATGACAGTA
AGAGAATTAT
GCAGTGCTGC
CATAACCATG
AGTGATAACA
CTGCGGCCAA
CTTACTTCTG
ACAACGATCG
GAGGACCGAA
GGAGCTAACC
GCTTTTTTGC
ACAACATGGG
GGATCATGTA
ACTCGCCTTG
ATCGTTGGGA
ACCGGAGCTG
AATGAAGCCA
TACCAAACGA
CGAGCGTGAC
ACCACGATGC
CTGTAGCAAT
GGCAACAACG
TTGCGCAAAC
TATTAACTGG
CGAACTACTT
ACTCTAGCTT
CCCGGCAACA
ATTAATAGAC
TGGATGGAGG
CGGATAAAGT
TGCAGGACCA
CTTCTGCGCT
CGGCCCTTCC
GGCTGGCTGG
TTTATTGCTG
ATAAATCTGG
AGCCGGTGAG
CGTGGGTCTC
GCGGTATCAT
TGCAGCACTG
GGGCCAGATG
GTAAGCCCTC
CCGTATCGTA
GTTATCTACA
CGACGGGGAG
TCAGGCAACT
ATGGATGAAC
GAAATAGACA
GATCGCTGAG
ATAGGTGCCT
CACTGATTAA
GCATTGGTAA
CTGTCAGACC
AAGTTTACTC
ATATATACTT
TAGATTGATT
TAAAACTTCA
TTTTTAATTT
AAAAGGATCT
AGGTGAAGAT
CCTTTTTGAT
AATCTCATGA
CCAAAATCCC
TTAACGTGAG
TTTTCGTTCC
ACTGAGCGTC
AGACCCCGTA
GAAAAGATCA
AAGGATCTTC
TTGAGATCCT
TTTTTTCTGC
GCGTAATCTG
CTGCTTGCAA
ACAAAAAAAC
CACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTTCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACGAC
CTACACCGAA
CTGAGATACC
TACAGCGTGA
GCTATGAGAA
AGCGCCACGC
TTCCCGAAGG
GAGAAAGGCG
GACAGGTATC
CGGTAAGCGG
CAGGGTCGGA
ACAGGAGAGC
GCACGAGGGA
GCTTCCAGGG
GGAAACGCCT
GGTATCTTTA
TAGTCCTGTC
GGGTTTCGCC
ACCTCTGACT
TGAGCGTCGA
TTTTTGTGAT
GCTCGTCAGG
GGGGCGGAGC
CTATGGAAAA
ACGCCAGCAA
CGCGGCCTTT
TTACGGTTCC
TGGCCTTTTG
CTGGCCTTTT
GCTCACATGT
TCTTTCCTGC
GTTATCCCCT
GATTCTGTGG
ATAACCGTAT
TACCGCCTTT
GAGTGAGCTG
ATACCGCTCG
CCGCAGCCGA
ACGACCGAGC
GCAGCGAGTC
AGTGAGCGAG
GAAGCGGAAG
AGCGCCCAAT
ACGCAAACCG
CCTCTCCCCG
CGCGTTGGCC
GATTCATTAA
TGCAGCTGGC
ACGACAGGTT
TCCCGACTGG
AAAGCGGGCA
GTGAGCGCAA
CGCAATTAAT
GTGAGTTAGC
TCACTCATTA
GGCACCCCAG
GCTTTACACT
TTATGCTTCC
GGCTCGTATG
TTGTGTGGAA
TTGTGAGCGG
ATAACAATTT
CACACAGGAA
ACAGCTATGA
CCATGATTAC
GCCAAGCTTG
CATGCCTGCA
GGTCGACTCT
AGACCTATAC
AGTTGAAGTC
GGAAGTTTAC
ATACACTTAA
GTTGGAGTCA
TTAAAACTCG
TTTTTCAACT
ACTCCACAAA
TTTCTTGTTA
ACAAACAATA
GTTTTGGCAA
GTCAGTTAGG
ACATCTACTT
TGTGCATGAC
ACAAGTCATT
TTTCCAACAA
TTGTTTACAG
ACAGATTATT
TCACTTATAA
TTCACTGTAT
CACAATTCCA
GTGGGTCAGA
AGTTTACATA
CACTAAGTTG
ACTGTGCCTT
TAAACAGCTT
GGAAAATTCC
AGAAAATGAT
GTCATGGCTT
TAGAAGCTTC
TGATAGACTA
ATTGACATCA
TTTGAGTCAA
TTGGAGGTGT
ACCTGTGGAT
GTATTTCAAG
GGGATCCAGA
CATGATAAGA
TACATTGATG
AGTTTGGACA
AACCACAACT
AGAATGCAGC
GGCCG