名称: | pLV3-CMV-Apoa4(rat)-3×FLAG-Puro |
---|---|
别称: | NM_012737.2;Apo-AIV; ApoA-IV; apoAIV; |
基因长: | 1173bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 大鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-Apoa4(rat)-3×FLAG-Puro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动下游大鼠源Apoa4基因转录,促进Apoa4基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCTAGCG
AATTCGCCAC
CATGTTCCTG
AAGGCTGTGG
TGCTGACCGT
GGCCCTGGTG
GCCATCACCG
GGACCCAGGC
TGAGGTCACT
TCCGACCAGG
TGGCCAATGT
GATGTGGGAC
TACTTCACCC
AGCTAAGCAA
CAATGCCAAG
GAGGCTGTGG
AACAACTGCA
GAAGACAGAT
GTCACTCAAC
AGCTCAATAC
CCTCTTCCAG
GACAAACTTG
GGAACATTAA
CACCTATGCC
GATGACCTGC
AGAACAAGCT
GGTGCCCTTC
GCCGTTCAAC
TGAGTGGACA
TCTAACCAAG
GAAACAGAGA
GGGTGAGGGA
AGAGATCCAG
AAGGAGCTCG
AGGACCTACG
GGCCAACATG
ATGCCCCATG
CCAACAAAGT
GAGCCAGATG
TTCGGGGACA
ACGTGCAGAA
GTTGCAGGAA
CACCTGAGGC
CCTATGCCAC
GGACCTGCAA
GCTCAGATCA
ACGCACAGAC
CCAGGATATG
AAACGCCAGC
TGACCCCCTA
CATCCAGCGC
ATGCAGACCA
CAATACAAGA
CAATGTGGAA
AACCTGCAGT
CCTCCATGGT
GCCCTTTGCC
AACGAGCTAA
AGGAAAAGTT
TAACCAGAAT
ATGGAAGGGC
TCAAGGGGCA
ACTAACCCCC
CGTGCCAACG
AGCTGAAGGC
CACGATCGAC
CAGAACCTGG
AAGACCTGCG
CAGCAGACTG
GCCCCTCTCG
CGGAGGGTGT
GCAGGAAAAA
CTCAACCATC
AGATGGAAGG
CTTGGCCTTC
CAGATGAAGA
AGAACGCTGA
GGAGCTCCAG
ACCAAGGTCT
CCACAAATAT
CGACCAGCTG
CAGAAGAATC
TGGCCCCGCT
GGTGGAAGAT
GTGCAAAGCA
AGCTGAAAGG
CAACACGGAA
GGACTGCAGA
AGTCTCTGGA
AGACCTGAAC
AAGCAGCTGG
ACCAGCAGGT
GGAGGTGTTC
CGGCGTGCTG
TGGAGCCCCT
GGGGGATAAG
TTCAACATGG
CTCTGGTGCA
GCAGATGGAG
AAGTTCAGGC
AGCAGCTGGG
CTCCGATTCG
GGGGACGTGG
AAAGCCACTT
GAGCTTCCTG
GAGAAGAACC
TGAGGGAAAA
GGTCAGCTCC
TTTATGAGCA
CCCTGCAAAA
AAAGGGGAGC
CCAGACCAGC
CCCTAGCCCT
CCCCCTCCCG
GAGCAGGTTC
AGGAACAGGT
CCAGGAGCAG
GTGCAGCCCA
AACCTCTGGA
GAGCGGATCC
GACTACAAAG
ACCATGACGG
TGATTATAAA
GATCATGACA
TCGACTACAA
GGATGACGAT
GACAAGTAGC
GGCCGCGAAG
GATCTGCGAT
CGCTCCGGTG
CCCGTCAGTG
GGCAGAGCGC
ACATCGCCCA
CAGTCCCCGA
GAAGTTGGGG
GGAGGGGTCG
GCAATTGAAC
GGGTGCCTAG
AGAAGGTGGC
GCGGGGTAAA
CTGGGAAAGT
GATGTCGTGT
ACTGGCTCCG
CCTTTTTCCC
GAGGGTGGGG
GAGAACCGTA
TATAAGTGCA
GTAGTCGCCG
TGAACGTTCT
TTTTCGCAAC
GGGTTTGCCG
CCAGAACACA
GCTGAAGCTT
CGAGGGGCTC
GCATCTCTCC
TTCACGCGCC
CGCCGCCCTA
CCTGAGGCCG
CCATCCACGC
CGGTTGAGTC
GCGTTCTGCC
GCCTCCCGCC
TGTGGTGCCT
CCTGAACTGC
GTCCGCCGTC
TAGGTAAGTT
TAAAGCTCAG
GTCGAGACCG
GGCCTTTGTC
CGGCGCTCCC
TTGGAGCCTA
CCTAGACTCA
GCCGGCTCTC
CACGCTTTGC
CTGACCCTGC
TTGCTCAACT
CTACGTCTTT
GTTTCGTTTT
CTGTTCTGCG
CCGTTACAGA
TCCAAGCTGT
GACCGGCGCC
TACGCTAGAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCC
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ACCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAGT
CGACAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAA
TCATCGTCCT
TTCCTTGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTTCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
GCCGCCTCCC
CGCCTGGTAC
CTTTAAGACC
AATGACTTAC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAAATAAGA
TCTGCTTTTT
GCTTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTAGTAGTTC
ATGTCATCTT
ATTATTCAGT
ATTTATAACT
TGCAAAGAAA
TGAATATCAG
AGAGTGAGAG
GAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGGCTCTAGC
TATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
GGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
ACTTTTGCAG
AGACGGCCCA
AATTCGTAAT
CATGGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGGAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCTAAGAAAC
CATTATTATC
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTCTCGC
GCGTTTCGGT
GATGACGGTG
AAAACCTCTG
ACACATGCAG
CTCCCGGAGA
CGGTCACAGC
TTGTCTGTAA
GCGGATGCCG
GGAGCAGACA
AGCCCGTCAG
GGCGCGTCAG
CGGGTGTTGG
CGGGTGTCGG
GGCTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGCGCCATTC
GCCATTCAGG
CTGCGCAACT
GTTGGGAAGG
GCGATCGGTG
CGGGCCTCTT
CGCTATTACG
CCAGCTGGCG
AAAGGGGGAT
GTGCTGCAAG
GCGATTAAGT
TGGGTAACGC
CAGGGTTTTC
CCAGTCACGA
CGTTGTAAAA
CGACGGCCAG
TGCCAAGCTG