名称: | p4412-EGFP |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 枯草杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Amp |
筛选标记: | Kan |
荧光标记: | 绿色 |
启动子: | p4412 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pBE2R-EGFP |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
p4412-EGFP 是一种用于基因表达研究的质粒载体,主要用于在真核细胞中表达增强型绿色荧光蛋白。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAGTGTCACC
TAAATCGTAT
GTGTATGATA
CATAAGGTTA
TGTATTAATT
GTAGCCGCGT
TCTAACGACA
ATATGTACAA
GCCTAATTGT
GTAGCATCTG
GCTTACTGAA
GCAGACCCTA
TCATCTCTCT
CGTAAACTGC
CGTCAGAGTC
GGTTTGGTTG
GACGAACCTT
CTGAGTTTCT
GGTAACGCCG
TTCCGCACCC
CGGAAATGGT
CAGCGAACCA
ATCAGCAGGG
TCATCGCTAG
CCAGATCCTC
TACGCCGGAC
GCATCGTGGC
CGGCATCACC
GGCGCCACAG
GTGCGGTTGC
TGGCGCCTAT
ATCGCCGACA
TCACCGATGG
GGAAGATCGG
GCTCGCCACT
TCGGGCTCAT
GAGCGCTTGT
TTCGGCGTGG
GTATGGTGGC
AGGCCCCGTG
GCCGGGGGAC
TGTTGGGCGC
CATCTCCTTG
CACCATTCCT
TGCGGCGGCG
GTGCTCAACG
GCCTCAACCT
ACTACTGGGC
TGCTTCCTAA
TGCAGGAGTC
GCATAAGGGA
GAGCGTCGAT
ATGGTGCACT
CTCAGTACAA
TCTGCTCTGA
TGCCGCATAG
TTAAGCCAGC
CCCGACACCC
GCCAACACCC
GCTGACGCGC
CCTGACGGGC
TTGTCTGCTC
CCGGCATCCG
CTTACAGACA
AGCTGTGACC
GTCTCCGGGA
GCTGCATGTG
TCAGAGGTTT
TCACCGTCAT
CACCGAAACG
CGCGAGACGA
AAGGGCCTCG
TGATACGCCT
ATTTTTATAG
GTTAATGTCA
TGATAATAAT
GGTTTCTTAG
ACGTCAGGTG
GCACTTTTCG
GGGAAATGTG
CGCGGAACCC
CTATTTGTTT
ATTTTTCTAA
ATACATTCAA
ATATGTATCC
GCTCATGAGA
CAATAACCCT
GATAAATGCT
TCAATAATAT
TGAAAAAGGA
AGAGTATGAG
TATTCAACAT
TTCCGTGTCG
CCCTTATTCC
CTTTTTTGCG
GCATTTTGCC
TTCCTGTTTT
TGCTCACCCA
GAAACGCTGG
TGAAAGTAAA
AGATGCTGAA
GATCAGTTGG
GTGCACGAGT
GGGTTACATC
GAACTGGATC
TCAACAGCGG
TAAGATCCTT
GAGAGTTTTC
GCCCCGAAGA
ACGTTTTCCA
ATGATGAGCA
CTTTTAAAGT
TCTGCTATGT
GGCGCGGTAT
TATCCCGTAT
TGACGCCGGG
CAAGAGCAAC
TCGGTCGCCG
CATACACTAT
TCTCAGAATG
ACTTGGTTGA
GTACTCACCA
GTCACAGAAA
AGCATCTTAC
GGATGGCATG
ACAGTAAGAG
AATTATGCAG
TGCTGCCATA
ACCATGAGTG
ATAACACTGC
GGCCAACTTA
CTTCTGACAA
CGATCGGAGG
ACCGAAGGAG
CTAACCGCTT
TTTTGCACAA
CATGGGGGAT
CATGTAACTC
GCCTTGATCG
TTGGGAACCG
GAGCTGAATG
AAGCCATACC
AAACGACGAG
CGTGACACCA
CGATGCCTGT
AGCAATGGCA
ACAACGTTGC
GCAAACTATT
AACTGGCGAA
CTACTTACTC
TAGCTTCCCG
GCAACAATTA
ATAGACTGGA
TGGAGGCGGA
TAAAGTTGCA
GGACCACTTC
TGCGCTCGGC
CCTTCCGGCT
GGCTGGTTTA
TTGCTGATAA
ATCTGGAGCC
GGTGAGCGTG
GGTCTCGCGG
TATCATTGCA
GCACTGGGGC
CAGATGGTAA
GCCCTCCCGT
ATCGTAGTTA
TCTACACGAC
GGGGAGTCAG
GCAACTATGG
ATGAACGAAA
TAGACAGATC
GCTGAGATAG
GTGCCTCACT
GATTAAGCAT
TGGTAACTGT
CAGACCAAGT
TTACTCATAT
ATACTTTAGA
TTGATTTAAA
ACTTCATTTT
TAATTTAAAA
GGATCTAGGT
GAAGATCCTT
TTTGATAATC
TCATGACCAA
AATCCCTTAA
CGTGAGTTTT
CGTTCCACTG
AGCGTCAGAC
CCCGTAGAAA
AGATCAAAGG
ATCTTCTTGA
GATCCTTTTT
TTCTGCGCGT
AATCTGCTGC
TTGCAAACAA
AAAAACCACC
GCTACCAGCG
GTGGTTTGTT
TGCCGGATCA
AGAGCTACCA
ACTCTTTTTC
CGAAGGTAAC
TGGCTTCAGC
AGAGCGCAGA
TACCAAATAC
TGTCCTTCTA
GTGTAGCCGT
AGTTAGGCCA
CCACTTCAAG
AACTCTGTAG
CACCGCCTAC
ATACCTCGCT
CTGCTAATCC
TGTTACCAGT
GGCTGCTGCC
AGTGGCGATA
AGTCGTGTCT
TACCGGGTTG
GACTCAAGAC
GATAGTTACC
GGATAAGGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCTA
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCCTGGC
CTTTTGCTGG
CCTTTTGCTC
ACATGTTCTT
TCCTGCGTTA
TCCCCTGATT
CTGTGGATAA
CCGTATTACC
GCCTTTGAGT
GAGCTGATAC
CGCTCGCCGC
AGCCGAACGA
CCGAGCGCAG
CGAGTCAGTG
AGCGAGGAAG
CGGAAGAGCG
CCCAATACGC
AAACCGCCTC
TCCCCGCGCG
TTGGCCGATT
CATTAATGCA
GCTGATTTCA
CTTTTTGCAT
TCTACAAACT
GCATAACTCA
TATGTAAATC
GCTCCTTTTT
AGGTGGCACA
AATGTGAGGC
ATTTTCGCTC
TTTCCGGCAA
CCACTTCCAA
GTAAAGTATA
ACACACTATA
CTTTATATTC
ATAAAGTGTG
TGCTCTGCGA
GGCTGTCGGC
AGTGCCGACC
AAAACCATAA
AACCTTTAAG
ACCTTTCTTT
TTTTTACGAG
AAAAAAGAAA
CAAAAAAACC
TGCCCTCTGC
CACCTCAGCA
AAGGGGGGTT
TTGCTCTCGT
GCTCGTTTAA
AAATCAGCAA
GGGACAGGTA
GTATTTTTTG
AGAAGATCAC
TCAAAAAATC
TCCACCTTTA
AACCCTTGCC
AATTTTTATT
TTGTCCGTTT
TGTCTAGCTT
ACCGAAAGCC
AGACTCAGCA
AGAATAAAAT
TTTTATTGTC
TTTCGGTTTT
CTAGTGTAAC
GGACAAAACC
ACTCAAAATA
AAAAAGATAC
AAGAGAGGTC
TCTCGTATCT
TTTATTCAGC
AATCGCGCCC
GATTGCTGAA
CAGATTAATA
ATAGATTTTA
GCTTTTTATT
TGTTGAAAAA
AGCTAATCAA
ATTGTTGTCG
GGATCAATTA
CTGCAAAGTC
TCGTTCATCC
CACCACTGAT
CTTTTAATGA
TGTATTGGGG
TGCAAAATGC
CCAAAGGCTT
AATATGTTGA
TATAATTCAT
CAATTCCCTC
TACTTCAATG
CGGCAACTAG
CAGTACCAGC
AATAAACGAC
TCCGCACCTG
TACAAACCGG
TGAATCATTA
CTACGAGAGC
GCCAGCTTCA
TCACTTGCCT
CCCATAGATG
AATCCGAACC
TCATTACACA
TTAGAACTGC
GAATCCATCT
TCATGGTGAA
CCAAAGTGAA
ACCTAGTTTA
TCGCAATAAA
AACCTATACT
CTTTTTAATA
TCCCCGACTG
GCAATGCCGG
GATAGACTGT
AACATTCTCA
CGCATAAAAT
CCCCTTTCAT
TTTCTAATGT
AAATCTATTA
CCTTATTATT
AATTCAATTC
GCTCATAATT
AATCCTTTTT
CTTATTACGC
AAAATGGCCC
GATTTAAGCA
CACCCTTTAT
TCCGTTAATG
CGCCATGACA
GCCATGATAA
TTACTAATAC
TAGGAGAAGT
TAATAAATAC
GTAACCAACA
TGATTAACAA
TTATTAGAGG
TCATCGTTCA
AAATGGTATG
CGTTTTGACA
CATCCACTAT
ATATCCGTGT
CGTTCTGTCC
ACTCCTGAAT
CCCATTCCAG
AAATTCTCTA
GCGATTCCAG
AAGTTTCTCA
GAGTCGGAAA
GTTGACCAGA
CATTACGAAC
TGGCACAGAT
GGTCATAACC
TGAAGGAAGA
TCTGATTGCT
TAACTGCTTC
AGTTAAGACC
GAAGCGCTCG
TCGTATAACA
GATGCGATGA
TGCAGACCAA
TCAACATGGC
ACCTGCCATT
GCTACCTGTA
CAGTCAAGGA
TGGTAGAAAT
GTTGTCGGTC
CTTGCACACG
AATATTACGC
CATTTGCCTG
CATATTCAAA
CAGCTCTTCT
ACGATAAGGG
CACAAATCGC
ATCGTGGAAC
GTTTGGGCTT
CTACCGATTT
AGCAGTTTGA
TACACTTTCT
CTAAGTATCC
ACCTGAATCA
TAAATCGGCA
AAATAGAGAA
AAATTGACCA
TGTGTAAGCG
GCCAATCTGA
TTCCACCTGA
GATGCATAAT
CTAGTAGAAT
CTCTTCGCTA
TCAAAATTCA
CTTCCACCTT
CCACTCACCG
GTTGTCCATT
CATGGCTGAA
CTCTGCTTCC
TCTGTTGACA
TGACACACAT
CATCTCAATA
TCCGAATAGG
GCCCATCAGT
CTGACGACCA
AGAGAGCCAT
AAACACCAAT
AGCCTTAACA
TCATCCCCAT
ATTTATCCAA
TATTCGTTCC
TTAATTTCAT
GAACAATCTT
CATTCTTTCT
TCTCTAGTCA
TTATTATTGG
TCCATTCACT
ATTCTCATTC
CCTTTTCAGA
TAATTTTAGA
TTTGCTTTTC
TAAATAAGAA
TATTTGGAGA
GCACCGTTCT
TATTCAGCTA
TTAATAACTC
GTCTTCCTAA
GCATCCTTCA
ATCCTTTTAA
TAACAATTAT
AGCATCTAAT
CTTCAACAAA
CTGGCCCGTT
TGTTGAACTA
CTCTTTAATA
AAATAATTTT
TCCGTTCCCA
ATTCCACATT
GCAATAATAG
AAAATCCATC
TTCATCGGCT
TTTTCGTCAT
CATCTGTATG
AATCAAATCG
CCTTCTTCTG
TGTCATCAAG
GTTTAATTTT
TTATGTATTT
CTTTTAACAA
ACCACCATAG
GAGATTAACC
TTTTACGGTG
TAAACCTTCC
TCCAAATCAG
ACAAACGTTT
CAAATTCTTT
TCTTCATCAT
CGGTCATAAA
ATCCGTATCC
TTTACAGGAT
ATTTTGCAGT
TTCGTCAATT
GCCGATTGTA
TATCCGATTT
ATATTTATTT
TTCGGTCGAA
TCATTTGAAC
TTTTACATTT
GGATCATAGT
CTAATTTCAT
TGCCTTTTTC
CAAAATTGAA
TCCATTGTTT
TTGATTCACG
TAGTTTTCTG
TATTCTTAAA
ATAAGTTGGT
TCCACACATA
CCAATACATG
CATGTGCTGA
TTATAAGAAT
TATCTTTATT
ATTTATTGTC
ACTTCCGTTG
CACGCATAAA
ACCAACAAGA
TTTTTATTAA
TTTTTTTATA
TTGCATCATT
CGGCGAAATC
CTTGAGCCAT
ATCTGACAAA
CTCTTATTTA
ATTCTTCGCC
ATCATAAACA
TTTTTAACTG
TTAATGTGAG
AAACAACCAA
CGAACTGTTG
GCTTTTGTTT
AATAACTTCA
GCAACAACCT
TTTGTGACTG
AATGCCATGT
TTCATTGCTC
TCCTCCAGTT
GCACATTGGA
CAAAGCCTGG
ATTTACAAAA
CCACACTCGA
TACAACTTTC
TTTCGCCTGT
TTCACGATTT
TGTTTATACT
CTAATATTTC
AGCACAATCT
TTTACTCTTT
CAGCCTTTTT
AAATTCAAGA
ATATGCAGAA
GTTCAAAGTA
ATCAACATTA
GCGATTTTCT
TTTCTCTCCA
TGGTCTCACT
TTTCCACTTT
TTGTCTTGTC
CACTAAAACC
CTTGATTTTT
CATCTGAATA
AATGCTACTA
TTAGGACACA
TAATATTAAA
AGAAACCCCC
ATCTATTTAG
TTATTTGTTT
AGTCACTTAT
AACTTTAACA
GATGGGGTTT
TTCTGTGCAA
CCAATTTTAA
GGGTTTTCAA
TACTTTAAAA
CACATACATA
CCAACACTTC
AACGCACCTT
TCAGCAACTA
AAATAAAAAT
GACGTTATTT
CTATATGTAT
CAAGATAAGA
AAGAACAAGT
TCAAAACCAT
CAAAAAAAGA
CACCTTTTCA
GGTGCTTTTT
TTATTTTATA
AACTCATTCC
CTGATCTCGA
CTTCGTTCTT
TTTTTACCTC
TCGGTTATGA
GTTAGTTCAA
ATTCGTTCTT
TTTAGGTTCT
AAATCGTGTT
TTTCTTGGAA
TTGTGCTGTT
TTATCCTTTA
CCTTGTCTAC
AAACCCCTTA
AAAACGTTTT
TAAAGGCTTT
TAAGCCGTCT
GTACGTTCCT
TAAGGAATTC
TTCCCAAGAC
CCTATTTATG
TGCAAATAAG
AAAACAATTA
AGCCAGCTTA
TTTTAAATGG
TGGTTTAAAG
GGTGGAGACC
AATTACCGTC
TATTCGTAGC
TTAGCGAAGG
AATTGCAAAT
TAGTGTAATT
ACAACGAAGC
GTGCGTACGA
GGAGCTTGAA
AAAGAAGGAT
ATATAGAAAC
TGTTGCTGGG
AAGGGGACTT
ATGTTTCACG
TAAAAATAAT
GAACTATTAA
AAGAACAGCG
GCTACGTCTA
TTGGAAAGTA
AGGCTGAAGA
AATCGTAAAA
GAAAGTAAAG
TGTTGCAGCT
TTCACTTGAA
GAATTGCAGC
AGATGATTGC
GTGTTTATAT
AAGGGGGAAT
AAGAGATGTT
AGAGCTAAAA
AATGTTTGTA
AAAGTTATGA
AGATTTTGCA
GTGAAAAATA
TAAGTTTTGC
ATTACCACGA
GGATATATTA
TGGGGTTTGT
CGGACCAAAT
GGTGCTGGGA
AAAGTACAAC
GATCATAGTG
TGCGCTGTTC
CTAGACAATC
TGCGTAGAAA
TATGCAATAA
AACTAGGCAA
AGGTACTTAC
CTTTGAACTT
TACGCTTGAA
TAGGTTGGTG
AAATCCCAAC
TCATCGTTAA
ATGTTGGTAA
CGATGCTCTG
AAACTCCGAC
GTGCATAAAT
AGTTAACTAC
ATTTGTGTAC
GAAGCTCGGT
AAAGGCGCTG
AAAAACAGAC
CTGAGAAGCG
GTTAGTAAAG
TAAGCGGGAG
GCTATAAAAC
CGTCATGCTG
ACTTACCAGG
AATCCTCGTA
TGTACGGCTC
GTAAATGCAA
AGGGAAACGA
AATGAAGTTT
CTCGCTTCTG
TAAAAGAAGT
TTAATCGTGG
GTTTTGTATT
TGTGGTTGCA
AAATCTATAC
TACTCCAATA
ATGGAATAGT
GTCTTGCGTC
CTACCAACAG
TGGACAAAAA
GATGATTAAC
GGGTCAAAGA
GGACAGCTAA
AACGGTAATG
AAAAGCTAAA
GCGTAAGGAA
CCATAGAGGA
TGATGACAAG
AGGAGTTAAA
CTCAATGCAT
CTAAATTGGA
GGAAATGACA
ATTGAATCTA
TGGGACAGCA
GCCCGTAGTA
GTGACGAAGT
TTCTGTAATG
GGAATAGAGC
GAAGGGGCAT
AGTCAGTCCA
ATTATTAAGT
TAAACCAATT
TGTTAGGGAA
GCCGTTTGAG
CATACCTGAT
TAAAACCTTG
CATTCTCCCA
ATAAGGAGGA
ATCGCAATGG
AACAAGAATC
TAACTATCTA
AAATCTGAAC
AAGAATTAAG
AGATAAACAG
GACGAATTAT
ATAATAAAAG
TTTCGAAAGT
ATAAATCAAA
AGGGACAATC
GTCTGGACAC
AAAAACTTAT
TAGAACTGAT
GACAAGTGAA
GCCACGATAC
TAACGGCTAT
CCATCGCATT
AAAGCGAATA
AGGGAAGTCG
TACGGCTGGA
AGTGACCATA
AATTAATGAA
ACCAGACATT
CTTCAAAAAG
ATTACCCAAA
AGTCGTACAA
CTCGTACAAG
AAAACTTTCG
AAATTATCGT
CCAGAACCTA
TTAAAAGAAA
GTACATTCCA
AAACCAAATT
CCAAAGAGTT
ACGCCCATTA
GGAATACCAA
GCATTATCGA
CCGAGTTATA
CAGGAGTGTG
TTCGTTTAAT
TATTGAACCA
ATTCTTGAGG
CGCAATTTTT
CCAACATTCA
TATGGATTCA
GACCAATGAG
AGATTCCAGT
AGTATGGCAT
TAGTTCGAAC
AACTGACATC
GTTCATATCA
CGGGACACTA
TTGGATTATC
GAAGGAGATA
TTAGTAAATT
TTTCGATTGT
GTTAACCATA
CGATTCTCAT
TAAGAAATTA
TGGAATATGG
GAATTAGGGA
TAGACGTGTC
CTCATGATTA
TCAAAGCAAT
GCTGAAAGCT
GGGATCATGG
ATGAATTAAA
AGAGAACCCA
ATTGGTACAC
CTCAAGGAGG
AATCATCTCT
CCGCTACTCG
CAAATGCTTA
TCTACACGCA
TTTGACCAAT
GGGTAACAAG
AGAATGGGAA
AATAAGAAAA
CAAGCTACCC
GTATGCACAA
AAATCCAAAC
AGTTCGAAAG
GTTACGGAAA
ACAACACGCC
TAAAAGAAAC
GTATTTGGTA
AGATATGCAG
ACGATTGGGT
ACTCATAACG
AACTCTAAAC
GGAATGCGGA
GAAATGGAAG
TACAAAATAA
CAAAATATCT
AAAAGAGCGG
TTGAAATTGA
CATTGTCAGA
AGAAAAGACG
CTCATAACAG
ATATACGAAG
ACGACCAATT
AAATTTCTTG
GCTTTGAAAT
CAAGGCAATT
AAGGGCAAGG
CGAAAAATGG
ACATATTCTC
GTCAGTAAGC
CAAATCAACA
ACGGTTAAAA
GAAAAGGTAA
ATGAGATTCA
TAAAACGATT
AAGAAAATCA
AAAAAGCAAC
AAGGAAAGAC
GACCTGATTC
ACTGGATTAA
TCTCATCAAC
TCTAAAATAA
GAGGGCTAAT
TCAATATTAC
GAATGTACAA
CGAAAGTGAA
TGTGTACATG
GGAAAGTATG
TTGATAAACT
CCACTATGCG
GCATATTTGA
AACTCCGAAA
ACATGGAGGT
TATAAAATAC
CTGCAAAACG
AGTGGACAAT
CTACGGTCAG
CCCACGAAAA
GTACGAAACC
AAAATACCAA
TGGTTGAATA
CAACGAATTG
AAAATAGGTG
TTACAAGGCT
AGATTTTTGT
AGGTGGAAAA
AGCCTAAAAA
TAAAAGCCCT
AAAGAGACAC
CGTATACCAA
ACTGGGTAGA
CAAATTTATA
TGAACCGAAC
GAATAAAAAG
CCCATTAAAG
TAAGAGCGGA
TGAGCTCCTC
AGCTTGAATT
GGTCCAAGAT
TATCAAAATC
GGACATAAGG
GAAAATTGTA
CAACTTTGAA
TACTTTATGA
ATCGAGCCTA
TGCATTTAAC
CGTGATAAAG
GAAGATGTCG
AGTATGTGGA
GAGTTACTTC
AGGGGGATGA
TGTACACATA
CACCATGTCA
ACCCAAAACT
CCCCATAGAA
GAAGTAAATC
GTGTTCCTAA
CCTTGCATCC
CTACATGAAC
ATTGCCATCA
AATGATTCAC
AGTTCAAAAG
ATTATTCCAT
GGTCGGAAAA
GTAATTTGGG
AAAAGGTTCT
AGGTTTTAGA
GAAAAATTGG
GATTAACGTA
AATAATTGAG
TTGATGGAAC
GCCGTATGAG
GTGAAAGTCT
CACGTACGGT
GTGAAGTGGG
GGAAAAGAAG
TCCAAACGAG
TAAAGTCGTG
GTTTTTTCTT
ACCTATCACT
ATTTATAATA
GGTGACCATA
AATTCGTGAG
TTTAAAGGAA
AAAGGTCATA
TTTAAGACTA
TGCTTTTTAC
TTATTTTGTT
TTATAATGTG
ATTTATGAGT
TTTTTGTAGA
AATTTATCTA
CAAAAAGGAT
AAAGATATAA
AAAACGTACT
GTTTTTATAA
TATAAAGTAA
GAAAAAATGA
GTCCGTGAAA
ACAAGAAAGG
AAGATAAATA
ATATGTTTGG
ATTTGGTGGA
TTTACTCGCG
ATCCTCTAGA
TTTAAGAAGG
AGATATACAT
GGATCCATGG
TGAGCAAGGG
CGAGGAGCTG
TTCACCGGGG
TGGTGCCCAT
CCTGGTCGAG
CTGGACGGCG
ACGTAAACGG
CCACAAGTTC
AGCGTGTCCG
GCGAGGGCGA
GGGCGATGCC
ACCTACGGCA
AGCTGACCCT
GAAGTTCATC
TGCACCACCG
GCAAGCTGCC
CGTGCCCTGG
CCCACCCTCG
TGACCACCCT
GACCTACGGC
GTGCAGTGCT
TCAGCCGCTA
CCCCGACCAC
ATGAAGCAGC
ACGACTTCTT
CAAGTCCGCC
ATGCCCGAAG
GCTACGTCCA
GGAGCGCACC
ATCTTCTTCA
AGGACGACGG
CAACTACAAG
ACCCGCGCCG
AGGTGAAGTT
CGAGGGCGAC
ACCCTGGTGA
ACCGCATCGA
GCTGAAGGGC
ATCGACTTCA
AGGAGGACGG
CAACATCCTG
GGGCACAAGC
TGGAGTACAA
CTACAACAGC
CACAACGTCT
ATATCATGGC
CGACAAGCAG
AAGAACGGCA
TCAAGGTGAA
CTTCAAGATC
CGCCACAACA
TCGAGGACGG
CAGCGTGCAG
CTCGCCGACC
ACTACCAGCA
GAACACCCCC
ATCGGCGACG
GCCCCGTGCT
GCTGCCCGAC
AACCACTACC
TGAGCACCCA
GTCCGCCCTG
AGCAAAGACC
CCAACGAGAA
GCGCGATCAC
ATGGTCCTGC
TGGAGTTCGT
GACCGCCGCC
GGGATCACTC
TCGGCATGGA
CGAGCTGTAC
AAGCTCGAGC
ACCACCACCA
CCACCACAAG
CTTGTATTCT
A