产品货号：P71907
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pCMV-CEP170(human-toxic-opt)-3×FLAG-Neo |
|---|---|
| 别称: | NM_001042404.2;FAM68A; KAB; KIAA0470; |
| 基因长: | 4458bp |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Neo/G418 |
| 荧光标记： | |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | DH5a |
| 温度: | 37度 |
| 背景载体: | pCMV-T7-MCS-3×FLAG-Neo |
| 正向引物: | CMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pCMV-CEP170(human-toxic-opt)-3×FLAG-Neo是适用于哺乳细胞过表达的质粒。质粒带有的CMV增强子和CMV启动子可联合启动经密码子优化的人源CEP170基因高效表达，促进CEP170C端融合表达3×FLAG标签，便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。质粒携带Neo/G418抗性基因，用于筛选出表达目的基因的宿主细胞。
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGGTA
CCGAGCTCGG
ATCCGCCACC
ATGAGCTTAA
CATCCTGGTT
TTTGGTGAGC
AGTGGAGGCA
CTCGCCACAG
GCTGCCACGA
GAAATGATTT
TTGTTGGAAG
AGATGACTGT
GAGCTCATGT
TGCAGTCTCG
TAGTGTGGAT
AAGCAACACG
CTGTCATCAA
CTATGATGCG
TCTACGGATG
AGCATTTAGT
GAAGGATTTG
GGCAGCCTCA
ATGGGACTTT
TGTGAATGAT
GTAAGGATTC
CGGAACAGAC
TTATATCACC
TTGAAACTTG
AAGATAAGCT
GAGATTTGGA
TATGATACAA
ATCTTTTCAC
TGTAGTACAA
GGAGAAATGA
GGGTCCCTGA
AGAAGCTCTT
AAGCATGAGA
AGTTTACCAT
TCAGCTTCAG
TTGTCCCAAA
AATCTTCAGA
ATCAGAATTA
TCCAAATCTG
CAAGTGCCAA
AAGCATAGAT
TCAAAGGTAG
CAGACGCTGC
TACTGAAGTG
CAGCACAAAA
CTACTGAAGC
ACTGAAATCC
GAGGAAAAAG
CCATGGATAT
TTCTGCTATG
CCCCGTGGTA
CTCCATTATA
TGGGCAGCCG
TCATGGTGGG
GGGATGATGA
GGTGGATGAA
AAAAGAGCTT
TCAAGACAAA
TGGCAAACCT
GAAGAAAAAA
ACCATGAAGC
TGGAACATCA
GGGTGCGGCA
TAGATGCCAA
GCAAGTTGAG
GAACAATCTG
CAGCTGCAAA
TGAAGAAGTA
CTTTTTCCTT
TCTGTAGGGA
ACCAAGTTAT
TTTGAAATCC
CTACAAAAGA
ATTCCAGCAA
CCATCACAAA
TAACAGAAAG
CACTATTCAT
GAAATCCCAA
CAAAAGACAC
GCCAAGTTCC
CATATAACAG
GTGCAGGGCA
TGCTTCATTT
ACCATTGAAT
TTGATGACAG
TACCCCAGGG
AAGGTAACTA
TTAGAGACCA
TGTGACAAAG
TTTACTTCTG
ATCAGCGCCA
CAAGTCCAAG
AAGTCTTCTC
CTGGAACTCA
AGACTTGCTG
GGGATTCAAA
CAGGAATGAT
GGCACCCGAA
AACAAAGTTG
CTGACTGGCT
AGCACAAAAC
AACCCTCCTC
AAATGCTATG
GGAAAGAACA
GAAGAGGATT
CTAAAAGCAT
TAAAAGTGAT
GTTCCAGTGT
ACTTGAAAAG
GTTGAAAGGA
AATAAACATG
ATGATGGTAC
GCAAAGTGAT
TCAGAGAACG
CTGGGGCTCA
CAGGCGCTGT
AGCAAACGTG
CAACTCTTGA
GGAACACTTA
AGACGCCACC
ATTCAGAACA
CAAAAAGCTA
CAGAAGGTCC
AGGCTACTGA
AAAGCATCAA
GACCAAGCTG
TTGTGTTTGG
AGTAGATGAC
AATCAGGATT
ATAATAGGCC
TGTTATCAAC
GAAAAACATA
AAGATCTAAT
AAAAGATTGG
GCTCTCAGTT
CTGCTGCAGC
AGTAATGGAA
GAAAGAAAAC
CACTGACTAC
ATCTGGATTT
CACCACTCAG
AGGAAGGCAC
ATCTTCATCT
GGAAGCAAAC
GTTGGGTTTC
ACAGTGGGCT
AGTTTGGCTG
CCAATCATAC
AAGGCATGAT
CAAGAAGAAA
GGATAATGGA
ATTTTCTGCA
CCTCTTCCTT
TAGAGAATGA
GACAGAGATC
AGTGAGTCTG
GCATGACCGT
GCGCAGCACT
GGCTCAGCAA
CCAGCCTGGC
CTCCCAGGGC
GAACGGCGCC
GCAGGACCCT
CCCCCAGCTG
CCCAATGAGG
AGAAGAGCCT
GGAATCACAC
CGGGCCAAGG
TGGTGACACA
GAGGTCCGAG
ATTGGCGAAA
AGCAGGATAC
AGAACTGCAG
GAAAAGGAAA
CCCCTACTCA
GGTGTACCAG
AAGGACAAGC
AGGACGCCGA
CAGACCTCTG
TCTAAAATGA
ATAGAGCCGT
GAATGGGGAG
ACACTGAAAA
CCGGCGGAGA
CAACAAGACC
CTGCTGCACC
TGGGCTCCTC
CGCCCCTGGC
AAGGAGAAGA
GCGAGACCGA
CAAGGAGACA
AGCCTGGTGA
AGCAGACCCT
GGCCAAGCTG
CAGCAGCAGG
AGCAGAGAGA
AGAGGCCCAG
TGGACCCCCA
CCAAGCTGTC
CAGCAAGAAC
GTTTCCGGCC
AGACAGACAA
ATGTCGGGAG
GAGACTTTCA
AGCAGGAAAG
CCAGCCCCCT
GAGAAGAACT
CCGGCCACTC
CACCTCTAAG
GGCGACAGAG
TTGCCCAGTC
CGAGTCCAAG
CGTAGGAAGG
CCGAAGAGAT
CCTGAAGAGC
CAGACACCCA
AGGGGGGAGA
TAAGAAAGAG
TCCTCCAAGA
GCCTCGTGAG
ACAGGGCTCC
TTCACCATCG
AGAAACCCTC
CCCCAATATT
CCCATCGAGC
TGATTCCTCA
CATTAACAAG
CAGACTTCCT
CCACACCCTC
CAGCCTGGCC
CTGACAAGCG
CCAGCCGGAT
TAGAGAGAGG
TCTGAATCCC
TGGATCCTGA
CTCCAGCATG
GACACAACTC
TGATCCTGAA
GGACACCGAG
GCCGTGATGG
CATTCCTGGA
GGCCAAGCTG
CGGGAGGACA
ACAAGACCGA
CGAGGGGCCA
GACACACCAT
CCTACAATAG
GGATAACTCC
ATCAGCCCAG
AGAGCGATGT
GGATACCGCC
AGCACCATCT
CCCTGGTGAC
CGGCGAAACC
GAGCGGAAAT
CCACCCAGAA
GAGAAAGTCT
TTCACCTCCC
TGTATAAGGA
TAGATGCAGT
ACCGGCTCTC
CATCAAAGGA
CGTGACTAAG
AGCTCCTCAA
GCGGCGCAAG
AGAGAAAATG
GAAAAGAAAA
CAAAAAGTCG
TTCCACAGAT
GTGGGTTCAA
GAGCAGATGG
TCGTAAATTT
GTTCAGTCCA
GTGGGAGAAT
AAGACAGCCC
TCAGTAGACT
TAACAGATGA
TGACCAAACC
TCTAGTGTAC
CTCATTCTGC
CATCTCTGAT
ATTATGTCAT
CTGATCAAGA
AACTTACTCT
TGTAAACCTC
ATGGACGGAC
TCCACTTACC
TCAGCTGATG
AGCATGTACA
TTCCAAACTG
GAAGGAAGTA
AAGTAACGAA
ATCTAAGACT
TCTCCGGTGG
TATCTGGTTC
ATCTAGTAAA
TCAACCACCC
TTCCAAGGCC
ACGACCTACC
AGGACTTCCC
TCTTGCGCAG
AGCACGACTT
GGTGAAGCTT
CAGACAGTGA
ACTTGCTGAT
GCTGACAAAG
CATCTGTTGC
TTCTGAAGTA
TCCACAACAA
GTTCTACATC
AAAACCTCCC
ACAGGAAGGC
GTAACATCTC
TCGGATTGAT
TTATTGGCTC
AGCCTCGTAG
AACACGACTT
GGCTCACTGT
CAGCTCGTAG
TGACTCTGAA
GCAACAATTT
CTAGAAGTAG
TGCCTCTTCG
AGGACCGCAG
AAGCCATCAT
TAGAAGTGGA
GCCAGACTAG
TACCATCAGA
TAAATTTTCT
CCTAGAATTA
GAGCTAACAG
TATCTCTCGA
CTCTCAGACT
CCAAGGTCAA
AAGTATGACC
TCAGCTCATG
GCTCTGCTTC
AGTAAATTCA
AGATGGAGGC
GCTTTCCTAC
TGATTATGCT
TCCACCTCAG
AAGATGAATT
TGGATCAAAC
CGTAATTCCC
CTAAACATAC
CCGTCTACGT
ACTTCTCCAG
CCCTGAAAAC
CACTCGCTTG
CAGAGCGCTG
GATCAGCAAT
GCCTACTAGT
TCTTCATTCA
AACACCGGAT
TAAAGAGCAG
GAAGACTACA
TCCGAGATTG
GACTGCTCAT
CGAGAAGAGA
TAGCCAGGAT
CAGCCAAGAT
CTTGCTCTCA
TTGCTCGGGA
GATCAACGAT
GTAGCAGGAG
AGATAGATTC
AGTGACTTCA
TCAGGCACTG
CCCCTAGTAC
CACAGTAAGC
ACTGCTGCCA
CCACCCCTGG
CTCTGCCATA
GACACTAGAG
AAGAGTTGGT
TGATCGTGTT
TTTGATGAAA
GCCTCAACTT
CCGAAAGATT
CCTCCATTAG
TTCATTCCAA
AACACCAGAA
GGAAACAACG
GTCGATCTGG
TGATCCAAGA
CCTCAAGCAG
CAGAGCCTCC
CGATCACTTA
ACAATTACAA
GGCGGAGAAC
CTGGAGCAGG
GATGAAGTCA
TGGGAGATAA
TCTGCTGCTG
TCATCCGTCT
TTCAGTTCTC
TAAGAAGATA
AGACAATCTA
TAGATAAGAC
AGCTGGAAAG
ATCAGAATAT
TATTTAAAGA
CAAAGATCGG
AATTGGGATG
ACATAGAAAG
CAAATTAAGA
GCCGAAAGTG
AAGTCCCTAT
TGTGAAAACC
TCAAGCATGG
AGATTTCTTC
TATCTTACAG
GAACTGAAAA
GAGTAGAAAA
GCAGCTGCAA
GCAATCAATG
CTATGATTGA
TCCTGATGGA
ACTTTGGAGG
CTCTGAACAA
CATGGGATTT
CCCAGTGCTA
TGTTGCCATC
TCCACCGAAA
CAGAAGTCCA
GCCCTGTGAA
TAACCACCAC
AGCCCGGGTC
AGACACCAAC
ACTTGGCCAA
CCAGAAGCTA
GGGCTCTTCA
TCCTGCTGCT
GTTTCAGCCG
CAGCTGAATT
TGAGAATGCT
GAATCTGAGG
CTGATTTCAG
TATACATTTC
AATAGATTCA
ACCCCGATGG
GGAAGAGGAA
GATGTTACAG
TACAAGAACT
CGAGGACTAC
AAAGACCATG
ACGGTGATTA
TAAAGATCAT
GACATCGACT
ACAAGGATGA
CGATGACAAG
TAGTGAGGGC
CCGTTTAAAC
CCGCTGATCA
GCCTCGACTG
TGCCTTCTAG
TTGCCAGCCA
TCTGTTGTTT
GCCCCTCCCC
CGTGCCTTCC
TTGACCCTGG
AAGGTGCCAC
TCCCACTGTC
CTTTCCTAAT
AAAATGAGGA
AATTGCATCG
CATTGTCTGA
GTAGGTGTCA
TTCTATTCTG
GGGGGTGGGG
TGGGGCAGGA
CAGCAAGGGG
GAGGATTGGG
AAGACAATAG
CAGGCATGCT
GGGGATGCGG
TGGGCTCTAT
GGCTTCTGAG
GCGGAAAGAA
CCAGCTGGGG
CTCTAGGGGG
TATCCCCACG
CGCCCTGTAG
CGGCGCATTA
AGCGCGGCGG
GTGTGGTGGT
TACGCGCAGC
GTGACCGCTA
CACTTGCCAG
CGCCCTAGCG
CCCGCTCCTT
TCGCTTTCTT
CCCTTCCTTT
CTCGCCACGT
TCGCCGGCTT
TCCCCGTCAA
GCTCTAAATC
GGGGGCTCCC
TTTAGGGTTC
CGATTTAGTG
CTTTACGGCA
CCTCGACCCC
AAAAAACTTG
ATTAGGGTGA
TGGTTCACGT
AGTGGGCCAT
CGCCCTGATA
GACGGTTTTT
CGCCCTTTGA
CGTTGGAGTC
CACGTTCTTT
AATAGTGGAC
TCTTGTTCCA
AACTGGAACA
ACACTCAACC
CTATCTCGGT
CTATTCTTTT
GATTTATAAG
GGATTTTGCC
GATTTCGGCC
TATTGGTTAA
AAAATGAGCT
GATTTAACAA
AAATTTAACG
CGAATTAATT
CTGTGGAATG
TGTGTCAGTT
AGGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCAGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCATAGT
CCCGCCCCTA
ACTCCGCCCA
TCCCGCCCCT
AACTCCGCCC
AGTTCCGCCC
ATTCTCCGCC
CCATGGCTGA
CTAATTTTTT
TTATTTATGC
AGAGGCCGAG
GCCGCCTCTG
CCTCTGAGCT
ATTCCAGAAG
TAGTGAGGAG
GCTTTTTTGG
AGGCCTAGGC
TTTTGCAAAA
AGCTCCCGGG
AGCTTGTATA
TCCATTTTCG
GATCTGATCA
AGAGACAGGA
TGAGGATCGT
TTCGCATGAT
TGAACAAGAT
GGATTGCACG
CAGGTTCTCC
GGCCGCTTGG
GTGGAGAGGC
TATTCGGCTA
TGACTGGGCA
CAACAGACAA
TCGGCTGCTC
TGATGCCGCC
GTGTTCCGGC
TGTCAGCGCA
GGGGCGCCCG
GTTCTTTTTG
TCAAGACCGA
CCTGTCCGGT
GCCCTGAATG
AACTGCAGGA
CGAGGCAGCG
CGGCTATCGT
GGCTGGCCAC
GACGGGCGTT
CCTTGCGCAG
CTGTGCTCGA
CGTTGTCACT
GAAGCGGGAA
GGGACTGGCT
GCTATTGGGC
GAAGTGCCGG
GGCAGGATCT
CCTGTCATCT
CACCTTGCTC
CTGCCGAGAA
AGTATCCATC
ATGGCTGATG
CAATGCGGCG
GCTGCATACG
CTTGATCCGG
CTACCTGCCC
ATTCGACCAC
CAAGCGAAAC
ATCGCATCGA
GCGAGCACGT
ACTCGGATGG
AAGCCGGTCT
TGTCGATCAG
GATGATCTGG
ACGAAGAGCA
TCAGGGGCTC
GCGCCAGCCG
AACTGTTCGC
CAGGCTCAAG
GCGCGCATGC
CCGACGGCGA
GGATCTCGTC
GTGACCCATG
GCGATGCCTG
CTTGCCGAAT
ATCATGGTGG
AAAATGGCCG
CTTTTCTGGA
TTCATCGACT
GTGGCCGGCT
GGGTGTGGCG
GACCGCTATC
AGGACATAGC
GTTGGCTACC
CGTGATATTG
CTGAAGAGCT
TGGCGGCGAA
TGGGCTGACC
GCTTCCTCGT
GCTTTACGGT
ATCGCCGCTC
CCGATTCGCA
GCGCATCGCC
TTCTATCGCC
TTCTTGACGA
GTTCTTCTGA
GCGGGACTCT
GGGGTTCGAA
ATGACCGACC
AAGCGACGCC
CAACCTGCCA
TCACGAGATT
TCGATTCCAC
CGCCGCCTTC
TATGAAAGGT
TGGGCTTCGG
AATCGTTTTC
CGGGACGCCG
GCTGGATGAT
CCTCCAGCGC
GGGGATCTCA
TGCTGGAGTT
CTTCGCCCAC
CCCAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGTATACC
GTCGACCTCT
AGCTAGAGCT
TGGCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGAACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTC
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