名称: | pEF1a-SCN5A(human)-8×His-Neo |
---|---|
别称: | NM_198056.3;CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1; |
基因长: | 6048bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pEF1a-MCS-Neo |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pEF1a-SCN5A(human)-8×His-Neo是适用于哺乳细胞过表达的质粒。质粒带有的EF1a启动子可启动人源SCN5A基因的高效表达,促进SCN5A基因C端融合表达8×His标签,便于融合蛋白与镍离子发生亲和作用进行蛋白纯化,也可使用抗His抗体来检测和识别目的蛋白。质粒携带Neo/G418抗性基因,用于筛选出表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGCGTTGACA
TTGATTATTG
ACTAGGCTTT
TGCAAAAAGC
TTTGCAAAGA
TGGATAAAGT
TTTAAACAGA
GAGGAATCTT
TGCAGCTAAT
GGACCTTCTA
GGTCTTGAAA
GGAGTGGGAA
TTGGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACCGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGGTAAGTG
CCGTGTGTGG
TTCCCGCGGG
CCTGGCCTCT
TTACGGGTTA
TGGCCCTTGC
GTGCCTTGAA
TTACTTCCAC
CTGGCTGCAG
TACGTGATTC
TTGATCCCGA
GCTTCGGGTT
GGAAGTGGGT
GGGAGAGTTC
GAGGCCTTGC
GCTTAAGGAG
CCCCTTCGCC
TCGTGCTTGA
GTTGAGGCCT
GGCCTGGGCG
CTGGGGCCGC
CGCGTGCGAA
TCTGGTGGCA
CCTTCGCGCC
TGTCTCGCTG
CTTTCGATAA
GTCTCTAGCC
ATTTAAAATT
TTTGATGACC
TGCTGCGACG
CTTTTTTTCT
GGCAAGATAG
TCTTGTAAAT
GCGGGCCAAG
ATCTGCACAC
TGGTATTTCG
GTTTTTGGGG
CCGCGGGCGG
CGACGGGGCC
CGTGCGTCCC
AGCGCACATG
TTCGGCGAGG
CGGGGCCTGC
GAGCGCGGCC
ACCGAGAATC
GGACGGGGGT
AGTCTCAAGC
TGGCCGGCCT
GCTCTGGTGC
CTGGCCTCGC
GCCGCCGTGT
ATCGCCCCGC
CCTGGGCGGC
AAGGCTGGCC
CGGTCGGCAC
CAGTTGCGTG
AGCGGAAAGA
TGGCCGCTTC
CCGGCCCTGC
TGCAGGGAGC
TCAAAATGGA
GGACGCGGCG
CTCGGGAGAG
CGGGCGGGTG
AGTCACCCAC
ACAAAGGAAA
AGGGCCTTTC
CGTCCTCAGC
CGTCGCTTCA
TGTGACTCCA
CGGAGTACCG
GGCGCCGTCC
AGGCACCTCG
ATTAGTTCTC
GAGCTTTTGG
AGTACGTCGT
CTTTAGGTTG
GGGGGAGGGG
TTTTATGCGA
TGGAGTTTCC
CCACACTGAG
TGGGTGGAGA
CTGAAGTTAG
GCCAGCTTGG
CACTTGATGT
AATTCTCCTT
GGAATTTGCC
CTTTTTGAGT
TTGGATCTTG
GTTCATTCTC
AAGCCTCAGA
CAGTGGTTCA
AAGTTTTTTT
CTTCCATTTC
AGGTGTCGTG
AGGAATTAGC
TTGGTACGCT
AGCGTTTAAA
CTTAAGCTTG
GTACCGCCAC
CATGGCAAAC
TTCCTATTAC
CTCGGGGCAC
CAGCAGCTTC
CGCAGGTTCA
CACGGGAGTC
CCTGGCAGCC
ATCGAGAAGC
GCATGGCAGA
GAAGCAAGCC
CGCGGCTCAA
CCACCTTGCA
GGAGAGCCGA
GAGGGGCTGC
CCGAGGAGGA
GGCTCCCCGG
CCCCAGCTGG
ACCTGCAGGC
CTCCAAAAAG
CTGCCAGATC
TCTATGGCAA
TCCACCCCAA
GAGCTCATCG
GAGAGCCCCT
GGAGGACCTG
GACCCCTTCT
ATAGCACCCA
AAAGACTTTC
ATCGTACTGA
ATAAAGGCAA
GACCATCTTC
CGGTTCAGTG
CCACCAACGC
CTTGTATGTC
CTCAGTCCCT
TCCACCCCAT
CCGGAGAGCG
GCTGTGAAGA
TTCTGGTTCA
CTCGCTCTTC
AACATGCTCA
TCATGTGCAC
CATCCTCACC
AACTGCGTGT
TCATGGCCCA
GCACGACCCT
CCACCCTGGA
CCAAGTATGT
CGAGTACACC
TTCACCGCCA
TTTACACCTT
TGAGTCTCTG
GTCAAGATTC
TGGCTCGAGG
CTTCTGCCTG
CACGCGTTCA
CTTTCCTTCG
GGACCCATGG
AACTGGCTGG
ACTTTAGTGT
GATTATCATG
GCATACACAA
CTGAATTTGT
GGACCTGGGC
AATGTCTCAG
CCTTACGCAC
CTTCCGAGTC
CTCCGGGCCC
TGAAAACTAT
ATCAGTCATT
TCAGGGCTGA
AGACCATCGT
GGGGGCCCTG
ATCCAGTCTG
TGAAGAAGCT
GGCTGATGTG
ATGGTCCTCA
CAGTCTTCTG
CCTCAGCGTC
TTTGCCCTCA
TCGGCCTGCA
GCTCTTCATG
GGCAACCTAA
GGCACAAGTG
CGTGCGCAAC
TTCACAGCGC
TCAACGGCAC
CAACGGCTCC
GTGGAGGCCG
ACGGCTTGGT
CTGGGAATCC
CTGGACCTTT
ACCTCAGTGA
TCCAGAAAAT
TACCTGCTCA
AGAACGGCAC
CTCTGATGTG
TTACTGTGTG
GGAACAGCTC
TGACGCTGGG
ACATGTCCGG
AGGGCTACCG
GTGCCTAAAG
GCAGGCGAGA
ACCCCGACCA
CGGCTACACC
AGCTTCGATT
CCTTTGCCTG
GGCCTTTCTT
GCACTCTTCC
GCCTGATGAC
GCAGGACTGC
TGGGAGCGCC
TCTATCAGCA
GACCCTCAGG
TCCGCAGGGA
AGATCTACAT
GATCTTCTTC
ATGCTTGTCA
TCTTCCTGGG
GTCCTTCTAC
CTGGTGAACC
TGATCCTGGC
CGTGGTCGCA
ATGGCCTATG
AGGAGCAAAA
CCAAGCCACC
ATCGCTGAGA
CCGAGGAGAA
GGAAAAGCGC
TTCCAGGAGG
CCATGGAAAT
GCTCAAGAAA
GAACACGAGG
CCCTCACCAT
CAGGGGTGTG
GATACCGTGT
CCCGTAGCTC
CTTGGAGATG
TCCCCTTTGG
CCCCAGTAAA
CAGCCATGAG
AGAAGAAGCA
AGAGGAGAAA
ACGGATGTCT
TCAGGAACTG
AGGAGTGTGG
GGAGGACAGG
CTCCCCAAGT
CTGACTCAGA
AGATGGTCCC
AGAGCAATGA
ATCATCTCAG
CCTCACCCGT
GGCCTCAGCA
GGACTTCTAT
GAAGCCACGT
TCCAGCCGCG
GGAGCATTTT
CACCTTTCGC
AGGCGAGACC
TGGGTTCTGA
AGCAGATTTT
GCAGATGATG
AAAACAGCAC
AGCGGGGGAG
AGCGAGAGCC
ACCACACATC
ACTGCTGGTG
CCCTGGCCCC
TGCGCCGGAC
CAGTGCCCAG
GGACAGCCCA
GTCCCGGAAC
CTCGGCTCCT
GGCCACGCCC
TCCATGGCAA
AAAGAACAGC
ACTGTGGACT
GCAATGGGGT
GGTCTCATTA
CTGGGGGCAG
GCGACCCAGA
GGCCACATCC
CCAGGAAGCC
ACCTCCTCCG
CCCTGTGATG
CTAGAGCACC
CGCCAGACAC
GACCACGCCA
TCGGAGGAGC
CAGGCGGGCC
CCAGATGCTG
ACCTCCCAGG
CTCCGTGTGT
AGATGGCTTC
GAGGAGCCAG
GAGCACGGCA
GCGGGCCCTC
AGCGCAGTCA
GCGTCCTCAC
CAGCGCACTG
GAAGAGTTAG
AGGAGTCTCG
CCACAAGTGT
CCACCATGCT
GGAACCGTCT
CGCCCAGCGC
TACCTGATCT
GGGAGTGCTG
CCCGCTGTGG
ATGTCCATCA
AGCAGGGAGT
GAAGTTGGTG
GTCATGGACC
CGTTTACTGA
CCTCACCATC
ACTATGTGCA
TCGTACTCAA
CACACTCTTC
ATGGCGCTGG
AGCACTACAA
CATGACAAGT
GAATTCGAGG
AGATGCTGCA
GGTCGGAAAC
CTGGTCTTCA
CAGGGATTTT
CACAGCAGAG
ATGACCTTCA
AGATCATTGC
CCTCGACCCC
TACTACTACT
TCCAACAGGG
CTGGAACATC
TTCGACAGCA
TCATCGTCAT
CCTTAGCCTC
ATGGAGCTGG
GCCTGTCCCG
CATGAGCAAC
TTGTCGGTGC
TGCGCTCCTT
CCGCCTGCTG
CGGGTCTTCA
AGCTGGCCAA
ATCATGGCCC
ACCCTGAACA
CACTCATCAA
GATCATCGGG
AACTCAGTGG
GGGCACTGGG
GAACCTGACA
CTGGTGCTAG
CCATCATCGT
GTTCATCTTT
GCTGTGGTGG
GCATGCAGCT
CTTTGGCAAG
AACTACTCGG
AGCTGAGGGA
CAGCGACTCA
GGCCTGCTGC
CTCGCTGGCA
CATGATGGAC
TTCTTTCATG
CCTTCCTCAT
CATCTTCCGC
ATCCTCTGTG
GAGAGTGGAT
CGAGACCATG
TGGGACTGCA
TGGAGGTGTC
GGGGCAGTCA
TTATGCCTGC
TGGTCTTCTT
GCTTGTTATG
GTCATTGGCA
ACCTTGTGGT
CCTGAATCTC
TTCCTGGCCT
TGCTGCTCAG
CTCCTTCAGT
GCAGACAACC
TCACAGCCCC
TGATGAGGAC
AGAGAGATGA
ACAACCTCCA
GCTGGCCCTG
GCCCGCATCC
AGAGGGGCCT
GCGCTTTGTC
AAGCGGACCA
CCTGGGATTT
CTGCTGTGGT
CTCCTGCGGC
AGCGGCCTCA
GAAGCCCGCA
GCCCTTGCCG
CCCAGGGCCA
GCTGCCCAGC
TGCATTGCCA
CCCCCTACTC
CCCGCCACCC
CCAGAGACGG
AGAAGGTGCC
TCCCACCCGC
AAGGAAACAC
GGTTTGAGGA
AGGCGAGCAA
CCAGGCCAGG
GCACCCCCGG
GGATCCAGAG
CCCGTGTGTG
TGCCCATCGC
TGTGGCCGAG
TCAGACACAG
ATGACCAAGA
AGAAGATGAG
GAGAACAGCC
TGGGCACGGA
GGAGGAGTCC
AGCAAGCAGC
AGGAATCCCA
GCCTGTGTCC
GGTGGCCCAG
AGGCCCCTCC
GGATTCCAGG
ACCTGGAGCC
AGGTGTCAGC
GACTGCCTCC
TCTGAGGCCG
AGGCCAGTGC
ATCTCAGGCC
GACTGGCGGC
AGCAGTGGAA
AGCGGAACCC
CAGGCCCCAG
GGTGCGGTGA
GACCCCAGAG
GACAGTTGCT
CCGAGGGCAG
CACAGCAGAC
ATGACCAACA
CCGCTGAGCT
CCTGGAGCAG
ATCCCTGACC
TCGGCCAGGA
TGTCAAGGAC
CCAGAGGACT
GCTTCACTGA
AGGCTGTGTC
CGGCGCTGTC
CCTGCTGTGC
GGTGGACACC
ACACAGGCCC
CAGGGAAGGT
CTGGTGGCGG
TTGCGCAAGA
CCTGCTACCA
CATCGTGGAG
CACAGCTGGT
TCGAGACATT
CATCATCTTC
ATGATCCTAC
TCAGCAGTGG
AGCGCTGGCC
TTCGAGGACA
TCTACCTAGA
GGAGCGGAAG
ACCATCAAGG
TTCTGCTTGA
GTATGCCGAC
AAGATGTTCA
CATATGTCTT
CGTGCTGGAG
ATGCTGCTCA
AGTGGGTGGC
CTACGGCTTC
AAGAAGTACT
TCACCAATGC
CTGGTGCTGG
CTCGACTTCC
TCATCGTAGA
CGTCTCTCTG
GTCAGCCTGG
TGGCCAACAC
CCTGGGCTTT
GCCGAGATGG
GCCCCATCAA
GTCACTGCGG
ACGCTGCGTG
CACTCCGTCC
TCTGAGAGCT
CTGTCACGAT
TTGAGGGCAT
GAGGGTGGTG
GTCAATGCCC
TGGTGGGCGC
CATCCCGTCC
ATCATGAACG
TCCTCCTCGT
CTGCCTCATC
TTCTGGCTCA
TCTTCAGCAT
CATGGGCGTG
AACCTCTTTG
CGGGGAAGTT
TGGGAGGTGC
ATCAACCAGA
CAGAGGGAGA
CTTGCCTTTG
AACTACACCA
TCGTGAACAA
CAAGAGCCAG
TGTGAGTCCT
TGAACTTGAC
CGGAGAATTG
TACTGGACCA
AGGTGAAAGT
CAACTTTGAC
AACGTGGGGG
CCGGGTACCT
GGCCCTTCTG
CAGGTGGCAA
CATTTAAAGG
CTGGATGGAC
ATTATGTATG
CAGCTGTGGA
CTCCAGGGGG
TATGAAGAGC
AGCCTCAGTG
GGAATACAAC
CTCTACATGT
ACATCTATTT
TGTCATTTTC
ATCATCTTTG
GGTCTTTCTT
CACCCTGAAC
CTCTTTATTG
GTGTCATCAT
TGACAACTTC
AACCAACAGA
AGAAAAAGTT
AGGGGGCCAG
GACATCTTCA
TGACAGAGGA
GCAGAAGAAG
TACTACAATG
CCATGAAGAA
GCTGGGCTCC
AAGAAGCCCC
AGAAGCCCAT
CCCACGGCCC
CTGAACAAGT
ACCAGGGCTT
CATATTCGAC
ATTGTGACCA
AGCAGGCCTT
TGACGTCACC
ATCATGTTTC
TGATCTGCTT
GAATATGGTG
ACCATGATGG
TGGAGACAGA
TGACCAAAGT
CCTGAGAAAA
TCAACATCTT
GGCCAAGATC
AACCTGCTCT
TTGTGGCCAT
CTTCACAGGC
GAGTGTATTG
TCAAGCTGGC
TGCCCTGCGC
CACTACTACT
TCACCAACAG
CTGGAATATC
TTCGACTTCG
TGGTTGTCAT
CCTCTCCATC
GTGGGCACTG
TGCTCTCGGA
CATCATCCAG
AAGTACTTCT
TCTCCCCGAC
GCTCTTCCGA
GTCATCCGCC
TGGCCCGAAT
AGGCCGCATC
CTCAGACTGA
TCCGAGGGGC
CAAGGGGATC
CGCACGCTGC
TCTTTGCCCT
CATGATGTCC
CTGCCTGCCC
TCTTCAACAT
CGGGCTGCTG
CTCTTCCTCG
TCATGTTCAT
CTACTCCATC
TTTGGCATGG
CCAACTTCGC
TTATGTCAAG
TGGGAGGCTG
GCATCGACGA
CATGTTCAAC
TTCCAGACCT
TCGCCAACAG
CATGCTGTGC
CTCTTCCAGA
TCACCACGTC
GGCCGGCTGG
GATGGCCTCC
TCAGCCCCAT
CCTCAACACT
GGGCCGCCCT
ACTGCGACCC
CACTCTGCCC
AACAGCAATG
GCTCTCGGGG
GGACTGCGGG
AGCCCAGCCG
TGGGCATCCT
CTTCTTCACC
ACCTACATCA
TCATCTCCTT
CCTCATCGTG
GTCAACATGT
ACATTGCCAT
CATCCTGGAG
AACTTCAGCG
TGGCCACGGA
GGAGAGCACC
GAGCCCCTGA
GTGAGGACGA
CTTCGATATG
TTCTATGAGA
TCTGGGAGAA
ATTTGACCCA
GAGGCCACTC
AGTTTATTGA
GTATTCGGTC
CTGTCTGACT
TTGCCGATGC
CCTGTCTGAG
CCACTCCGTA
TCGCCAAGCC
CAACCAGATA
AGCCTCATCA
ACATGGACCT
GCCCATGGTG
AGTGGGGACC
GCATCCATTG
CATGGACATT
CTCTTTGCCT
TCACCAAAAG
GGTCCTGGGG
GAGTCTGGGG
AGATGGACGC
CCTGAAGATC
CAGATGGAGG
AGAAGTTCAT
GGCAGCCAAC
CCATCCAAGA
TCTCCTACGA
GCCCATCACC
ACCACACTCC
GGCGCAAGCA
CGAAGAGGTG
TCGGCCATGG
TTATCCAGAG
AGCCTTCCGC
AGGCACCTGC
TGCAACGCTC
TTTGAAGCAT
GCCTCCTTCC
TCTTCCGTCA
GCAGGCGGGC
AGCGGCCTCT
CCGAAGAGGA
TGCCCCTGAG
CGAGAGGGCC
TCATCGCCTA
CGTGATGAGT
GAGAACTTCT
CCCGACCCCT
TGGCCCACCC
TCCAGCTCCT
CCATCTCCTC
CACTTCCTTC
CCACCCTCCT
ATGACAGTGT
CACTAGAGCC
ACCAGCGATA
ACCTCCAGGT
GCGGGGGTCT
GACTACAGCC
ACAGTGAAGA
TCTCGCCGAC
TTCCCCCCTT
CTCCGGACAG
GGACCGTGAG
TCCATCGTGC
TCGAGCACCA
TCACCATCAC
CATCACCATT
AGGCGGCCGC
GACTCTAGAT
CATAATCAGC
CATACCACAT
TTGTAGAGGT
TTTACTTGCT
TTAAAAAACC
TCCCACACCT
CCCCCTGAAC
CTGAAACATA
AAATGAATGC
AATTGTTGTT
GTTAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTAAGGCG
TAAATTGTAA
GCGTTAATAT
TTTGTTAAAA
TTCGCGTTAA
ATTTTTGTTA
AATCAGCTCA
TTTTTTAACC
AATAGGCCGA
AATCGGCAAA
ATCCCTTATA
AATCAAAAGA
ATAGACCGAG
ATAGGGTTGA
GTGTTGTTCC
AGTTTGGAAC
AAGAGTCCAC
TATTAAAGAA
CGTGGACTCC
AACGTCAAAG
GGCGAAAAAC
CGTCTATCAG
GGCGATGGCC
CACTACGTGA
ACCATCACCC
TAATCAAGTT
TTTTGGGGTC
GAGGTGCCGT
AAAGCACTAA
ATCGGAACCC
TAAAGGGAGC
CCCCGATTTA
GAGCTTGACG
GGGAAAGCCG
GCGAACGTGG
CGAGAAAGGA
AGGGAAGAAA
GCGAAAGGAG
CGGGCGCTAG
GGCGCTGGCA
AGTGTAGCGG
TCACGCTGCG
CGTAACCACC
ACACCCGCCG
CGCTTAATGC
GCCGCTACAG
GGCGCGTCAG
GTGGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AGACAATAAC
CCTGATAAAT
GCTTCAATAA
TATTGAAAAA
GGAAGAGTCC
TGAGGCGGAA
AGAACCAGCT
GTGGAATGTG
TGTCAGTTAG
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CAGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCGGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTT
TTGCAAAGAT
CGATCAAGAG
ACAGGATGAG
GATCGTTTCG
CATGATTGAA
CAAGATGGAT
TGCACGCAGG
TTCTCCGGCC
GCTTGGGTGG
AGAGGCTATT
CGGCTATGAC
TGGGCACAAC
AGACAATCGG
CTGCTCTGAT
GCCGCCGTGT
TCCGGCTGTC
AGCGCAGGGG
CGCCCGGTTC
TTTTTGTCAA
GACCGACCTG
TCCGGTGCCC
TGAATGAACT
GCAAGACGAG
GCAGCGCGGC
TATCGTGGCT
GGCCACGACG
GGCGTTCCTT
GCGCAGCTGT
GCTCGACGTT
GTCACTGAAG
CGGGAAGGGA
CTGGCTGCTA
TTGGGCGAAG
TGCCGGGGCA
GGATCTCCTG
TCATCTCACC
TTGCTCCTGC
CGAGAAAGTA
TCCATCATGG
CTGATGCAAT
GCGGCGGCTG
CATACGCTTG
ATCCGGCTAC
CTGCCCATTC
GACCACCAAG
CGAAACATCG
CATCGAGCGA
GCACGTACTC
GGATGGAAGC
CGGTCTTGTC
GATCAGGATG
ATCTGGACGA
AGAGCATCAG
GGGCTCGCGC
CAGCCGAACT
GTTCGCCAGG
CTCAAGGCGA
GCATGCCCGA
CGGCGAGGAT
CTCGTCGTGA
CCCATGGCGA
TGCCTGCTTG
CCGAATATCA
TGGTGGAAAA
TGGCCGCTTT
TCTGGATTCA
TCGACTGTGG
CCGGCTGGGT
GTGGCGGACC
GCTATCAGGA
CATAGCGTTG
GCTACCCGTG
ATATTGCTGA
AGAGCTTGGC
GGCGAATGGG
CTGACCGCTT
CCTCGTGCTT
TACGGTATCG
CCGCTCCCGA
TTCGCAGCGC
ATCGCCTTCT
ATCGCCTTCT
TGACGAGTTC
TTCTGAGCGG
GACTCTGGGG
TTCGAAATGA
CCGACCAAGC
GACGCCCAAC
CTGCCATCAC
GAGATTTCGA
TTCCACCGCC
GCCTTCTATG
AAAGGTTGGG
CTTCGGAATC
GTTTTCCGGG
ACGCCGGCTG
GATGATCCTC
CAGCGCGGGG
ATCTCATGCT
GGAGTTCTTC
GCCCACCCTA
GGGGGAGGCT
AACTGAAACA
CGGAAGGAGA
CAATACCGGA
AGGAACCCGC
GCTATGACGG
CAATAAAAAG
ACAGAATAAA
ACGCACGGTG
TTGGGTCGTT
TGTTCATAAA
CGCGGGGTTC
GGTCCCAGGG
CTGGCACTCT
GTCGATACCC
CACCGAGACC
CCATTGGGGC
CAATACGCCC
GCGTTTCTTC
CTTTTCCCCA
CCCCACCCCC
CAAGTTCGGG
TGAAGGCCCA
GGGCTCGCAG
CCAACGTCGG
GGCGGCAGGC
CCTGCCATAG
CCTCAGGTTA
CTCATATATA
CTTTAGATTG
ATTTAAAACT
TCATTTTTAA
TTTAAAAGGA
TCTAGGTGAA
GATCCTTTTT
GATAATCTCA
TGACCAAAAT
CCCTTAACGT
GAGTTTTCGT
TCCACTGAGC
GTCAGACCCC
GTAGAAAAGA
TCAAAGGATC
TTCTTGAGAT
CCTTTTTTTC
TGCGCGTAAT
CTGCTGCTTG
CAAACAAAAA
AACCACCGCT
ACCAGCGGTG
GTTTGTTTGC
CGGATCAAGA
GCTACCAACT
CTTTTTCCGA
AGGTAACTGG
CTTCAGCAGA
GCGCAGATAC
CAAATACTGT
TCTTCTAGTG
TAGCCGTAGT
TAGGCCACCA
CTTCAAGAAC
TCTGTAGCAC
CGCCTACATA
CCTCGCTCTG
CTAATCCTGT
TACCAGTGGC
TGCTGCCAGT
GGCGATAAGT
CGTGTCTTAC
CGGGTTGGAC
TCAAGACGAT
AGTTACCGGA
TAAGGCGCAG
CGGTCGGGCT
GAACGGGGGG
TTCGTGCACA
CAGCCCAGCT
TGGAGCGAAC
GACCTACACC
GAACTGAGAT
ACCTACAGCG
TGAGCTATGA
GAAAGCGCCA
CGCTTCCCGA
AGGGAGAAAG
GCGGACAGGT
ATCCGGTAAG
CGGCAGGGTC
GGAACAGGAG
AGCGCACGAG
GGAGCTTCCA
GGGGGAAACG
CCTGGTATCT
TTATAGTCCT
GTCGGGTTTC
GCCACCTCTG
ACTTGAGCGT
CGATTTTTGT
GATGCTCGTC
AGGGGGGCGG
AGCCTATGGA
AAAACGCCAG
CAACGCGGCC
TTTTTACGGT
TCCTGGCCTT
TTGCTGGCCT
TTTGCTCACA
TGT