名称: | pCMV-Ttll4(mouse)-3×FLAG-Neo |
---|---|
别称: | NM_001014974.2;4632407P03Rik; mKIAA0173 |
基因长: | 3579bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-T7-MCS-3×FLAG-WPRE-Neo |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pCMV-Ttll4(mouse)-3×FLAG-Neo是适用于哺乳细胞过表达的质粒。质粒带有的CMV增强子和CMV启动子可联合启动小鼠源Ttll4基因高效表达,促进Ttll4基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。WPRE蛋白可增强目的基因转录后的加工和稳定性,有助于增加mRNA的半衰期和翻译效率。质粒携带Neo/G418抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTAGGCGTT
TTGCGCTGCT
TCGCGATGTA
CGGGCCAGAT
ATACGCGTTG
ACATTGATTA
TTGACTAGTT
ATTAATAGTA
ATCAATTACG
GGGTCATTAG
TTCATAGCCC
ATATATGGAG
TTCCGCGTTA
CATAACTTAC
GGTAAATGGC
CCGCCTGGCT
GACCGCCCAA
CGACCCCCGC
CCATTGACGT
CAATAATGAC
GTATGTTCCC
ATAGTAACGC
CAATAGGGAC
TTTCCATTGA
CGTCAATGGG
TGGAGTATTT
ACGGTAAACT
GCCCACTTGG
CAGTACATCA
AGTGTATCAT
ATGCCAAGTA
CGCCCCCTAT
TGACGTCAAT
GACGGTAAAT
GGCCCGCCTG
GCATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTCTA
TATAAGCAGA
GCTCTCTGGC
TAACTAGAGA
ACCCACTGCT
TACTGGCTTA
TCGAAATTAA
TACGACTCAC
TATAGGGAGA
CCCAAGCTGG
CTAGCGTTTA
AACTTAAGCT
TGGTACCGAG
CTCGAATTCG
CCACCATGGC
CTCAGCAGGA
ACAGAGCACT
ACAGTATTGG
CCTGCGCCGA
GGAAACAGCT
TCAAGCAGAG
ACATCCCTCA
GGCACAGTGT
CTGCTTCACC
ATCTGAGAAA
CCCTCGGAGG
TCAAAGTCTG
GTCTCAGGCC
CATCAGCAGG
TGAAGCCAAT
CTGGAAACTG
GAAAAGAAGC
ACGTAGGCAC
ACTGTCAGCA
GGGCTGGGCA
CCAGTTTCTT
GGGAGTCCCA
TCCCAGCCAG
CCTATTTCCT
TTGCCCTAGC
ACTTTATGTA
GCTCTGGGAC
CACAGCAGTC
ATTGCAGGCC
ACAGCAACCC
CTGTTACCTG
CAGTCCCTCC
CTAACTTGTT
CAGCAATACT
CTGCTGTACC
GCCGCACCAA
CGTGAGGCAA
AAACCATACC
AGCAACTGGA
ATCTTTCTGC
TTGCGTTCCA
GCCCTTCAGA
GAAAAGATCT
TTTTCTCTCC
CTCAGAAGGG
TCTTCCTGTC
AGTGTCACTG
CCAATAAAGC
CACATCTTCT
ACAGTCTTCC
CCATGGCCCA
GCCCATGGCA
ACTTCACCCA
CTGATCCATA
CCTCTCACTG
GCAGCAGCTG
GGGAGAACCC
TTCAAGGAAG
AGCCTGGCCT
CTGCTATCTC
AGGGAAGATT
GCATCTCCAC
TGTCCTATAA
GCCCATGCTG
AACAACAACT
CCTTCATGAG
GCCAAATAGC
ACTAAAGTGC
CCTTATCACA
AGCCACAGAT
GGCCTGAAGC
CAGTATCCTC
ACCCAAGATC
CAGCCTGTCT
CATGGCATCA
TTCAGGGGGT
ACTGGGGACT
GTGTACCCCA
ACCTGGTGAC
CATAAGGTGC
CCCAAAACAT
TGCTACTGTC
TTAGATGATG
TCACTGCCCC
TATCACCCCA
TCTATCCCTA
GCACCCTCAA
CATATCCACA
GCCAGTGTTA
CCTCTTCCCA
GTGCAGTCAG
AGTAACTTTA
GGATGGAGGC
ACATCCCTGT
GGCCTGGATG
AAAACCCGGA
TTCCCAGAGT
GCTACTAAAG
AGGTACACTT
CACTGAGGCT
GTGAGGAAGT
TGGCTGAAAA
AGGCTTGGAG
AAGATGCCAC
GACAAGGCTA
CCAGTTTGAA
CAGGCTTGTT
TTGTGAACCC
CAGCTTCCAG
TGGGGTCTTC
TCAACAGAAG
CAGGCGGTGG
AAACCTCTGA
TGGGCCAGCG
GTTTCCTCAG
GAGGATATTG
GATTAGACAG
TGCGATCCTT
CCTGGTACTT
CAGACACCTT
GGGGTTGGAC
AGCACAGTCT
TCTGTACCAA
GAGGATCAGC
ATTCACCTCC
TTGCCTCACA
TGTCCATGGG
CTCAATCCCA
GCCCTGCCTG
TGGGTCTGCA
GTTGACCCCC
AGGTACTTGG
AGAAGACAGA
GCTCCTGTTC
CCCCTTCTTC
CCTCCAGCCT
CTTGGTGTAG
CTGAAGTGGC
CACTCGTCTT
TCTTCTGTCC
ATCTTGACCA
GCCTGGGAAG
GAGCCTGAGG
AAGCCAAGGA
CCTGAACTCT
TGTACTAAGG
GTGGTGGTTC
AGCTACTGAC
CTCCAGCCAA
ACCAGGTGGA
GCCTGAAGAT
ACAGAAGATG
AACTGGGAGA
TGGTTTGGAA
GATAGCTGCA
GCCATGATGA
GAATGAAGAA
GAGGAAGGAG
ACTCAGAGTG
CTCTTCTTTA
AGTGTTGTCT
CCCCCAGCGA
GTCAGTGGCA
TTAATATCTC
GGAACTGTGT
GGATTTGATG
TCCAAATCCC
TTCCCAATCA
TGAGAAAGTT
GTACGGCCAG
CCCTCATCTA
CAGTCTCTTC
CCCAATGTAA
CCCCTACCAT
CTATTTTGGG
ACTCGGGATG
AAAGAGTGGA
GAAACTTCCT
TGGGAACAGA
GGAGGCTGCT
TCGGTGGAAG
ATGAGCACAG
TGACCCCCAA
CATTGTCAAG
CAGACCATCG
GTCGGTCACA
CTTCAAAATC
AGCAAGAGAA
ATGATGACTG
GCTGGGCTGC
TGGGGTCACC
ACATGAAGTC
TCCTGGTTTT
CGATCCATTA
GAGAGCATCA
GAAGCTAAAC
CACTTCCCAG
GCTCCTTCCA
GATTGGGAGG
AAGGACCGGC
TGTGGCGGAA
CCTGTCTCGC
ATGCAAAGCC
GTTTTGGCAA
GAAGGAGTTC
AGTTTCTTTC
CTCAGTCCTT
TATCCTGCCC
CAGGATTCCA
AGCTCTTGCG
CAAAGCCTGG
GAGAGCAGCA
GCCGCCAGAA
GTGGATTGTG
AAGCCACCAG
CATCTGCTCG
AGGCATTGGC
ATTCAGGTCA
TTCACAAATG
GAGTCAGCTC
CCCAAGCGCA
GGCCCCTCCT
GGTACAGAGG
TATCTACACA
AGCCCTACCT
CATTAGTGGC
AGCAAATTTG
ATCTTCGGAT
CTATGTTTAC
GTGACTTCTT
ATGATCCTCT
ACGGATTTAC
CTCTTTTCGG
ATGGACTCGT
CCGTTTTGCC
AGTTGCAAGT
ATTCCCCTTC
CATGAAGAGT
CTTAGCAACA
AGTTCATGCA
CCTGACCAAC
TACAGTGTCA
ATAAAAAGAA
CACAGAATAC
CAGGCTAATG
CTGATGAGAC
TGCATGCCAA
GGGCACAAAT
GGGCATTGAA
AGCTTTGTGG
AACTATCTGA
GCCAGAAAGG
AATTAATAGT
GATGCCATCT
GGGAGAAGAT
AAAGGATGTT
GTAGTCAAAA
CCATCATCTC
GTCAGAGCCC
TATGTGACTA
ACCTACTGAA
GTTATATGTG
CGGCGCCCCT
ATAGCTGTCA
TGAGCTCTTT
GGTTTTGACA
TCATGCTGGA
TGAGAACCTG
AAACCCTGGG
TCCTAGAAGT
CAACATTTCT
CCAAGCCTCC
ATTCCAACTC
TCCTCTGGAC
ATCAGCATCA
AAGGCCAGAT
GATCCGAGAC
CTTCTGAACC
TGGCTGGCTT
TGTTCTGCCC
AACATGGAGG
ATATCATCTC
TAGTTCCAGC
AGCCCCAGCA
GCTCCAGCGG
CTCCAGCACC
AGCTTGCCAA
GCTCTCCTAG
AGACAAATGT
CAAATGACTC
CAGAGCACTT
CACTGCACAG
AAGATGAAGA
AAGCCTATTA
CTTGACCCAG
AAAATTCCTG
ACCAGGACTT
CTATGCGTCC
GTGCTGGACG
TCTTGACACC
AGATGATGTT
CGGGTTTTGG
TGGAGATGGA
AGATGAGTTT
TCTCGTCGTG
GTCAATTTGA
AAGAATTTTT
CCTTCCCGAA
TCTCTTCTCG
ATATCTTCGG
TTTTTTGAGC
AGCCACGATA
TTTCAACATT
CTCACCACCC
AGTGGGAACA
GAAGTACCAT
GGAAACAAGC
TCAAAGGAGT
AGATCTGCTT
CGGAATTGGT
GCTACAAAGG
CTTCCACACG
GGGATTGTCT
CTGACTCAGC
CCCACTGTGG
TCTCTGCCAA
CATCGCTCAT
GACTACCTCA
AAGGGTGATG
GGACACCAAA
TTCCGCCAGC
AAGTCACGAA
AGAAGAGCGC
CTCTGAGGGA
ACCACACTGT
CCTCTGAAGA
CAGGTCAACA
CCCAAGTCTA
AGAAGAGTCA
AGCTGGCCTG
TCTCCCATAT
CCAGGAAGAC
TTTGTCCTCA
AGGAGCAATG
AGAACACCAG
CAAACAGTCC
AAGCGCTCTA
CCCCGGGGTT
ACCTGTCCTC
AAGTACTCTG
GGCAGAGTTC
CAGACTTTCT
GCTGCTTCTG
CTTCCCAGTC
AGTCACTGAC
TCCCGCTTGA
CTGCTGTGAG
CTCGGGATCC
GACTACAAAG
ACCATGACGG
TGATTATAAA
GATCATGACA
TCGACTACAA
GGATGACGAT
GACAAGTAGT
GAGGGCCCGA
TATCTCGACA
ATCAACCTCT
GGATTACAAA
ATTTGTGAAA
GATTGACTGG
TATTCTTAAC
TATGTTGCTC
CTTTTACGCT
ATGTGGATAC
GCTGCTTTAA
TGCCTTTGTA
TCATGCTATT
GCTTCCCGTA
TGGCTTTCAT
TTTCTCCTCC
TTGTATAAAT
CCTGGTTGCT
GTCTCTTTAT
GAGGAGTTGT
GGCCCGTTGT
CAGGCAACGT
GGCGTGGTGT
GCACTGTGTT
TGCTGACGCA
ACCCCCACTG
GTTGGGGCAT
TGCCACCACC
TGTCAGCTCC
TTTCCGGGAC
TTTCGCTTTC
CCCCTCCCTA
TTGCCACGGC
GGAACTCATC
GCCGCCTGCC
TTGCCCGCTG
CTGGACAGGG
GCTCGGCTGT
TGGGCACTGA
CAATTCCGTG
GTGTTGTCGG
GGAAGCTGAC
GTCCTTTCCA
TGGCTGCTCG
CCTGTGTTGC
CACCTGGATT
CTGCGCGGGA
CGTCCTTCTG
CTACGTCCCT
TCGGCCCTCA
ATCCAGCGGA
CCTTCCTTCC
CGCGGCCTGC
TGCCGGCTCT
GCGGCCTCTT
CCGCGTCTTC
GCCTTCGCCC
TCAGACGAGT
CGGATCTCCC
TTTGGGCCGC
CTCCCCGCCT
GGAAACGGGG
GAGGCTAACT
GAAACACGGA
AGGAGACAAT
ACCGGAAGGA
ACCCGCGCTA
TGACGGCAAT
AAAAAGACAG
AATAAAACGC
ACGGGTGTTG
GGTCGTTTGT
TCATAAACGC
GGGGTTCGGT
CCCAGGGCTG
GCACTCTGTC
GATACCCCAC
CGAGACCCCA
TTGGGGCCAA
TACGCCCGCG
TTTCTTCCTT
TTCCCCACCC
CACCCCCCAA
GTTCGGGTGA
AGGCCCAGGG
CTCGCAGCCA
ACGTCGGGGC
GGCAGGCCCT
GCCATAGCAG
ATCTGCGCAG
CTGGGGCTCT
AGGGGGTATC
CCCACGCGCC
CTGTAGCGGC
GCATTAAGCG
CGGCGGGTGT
GGTGGTTACG
CGCAGCGTGA
CCGCTACACT
TGCCAGCGCC
CTAGCGCCCG
CTCCTTTCGC
TTTCTTCCCT
TCCTTTCTCG
CCACGTTCGC
CGGCTTTCCC
CGTCAAGCTC
TAAATCGGGG
CATCCCTTTA
GGGTTCCGAT
TTAGTGCTTT
ACGGCACCTC
GACCCCAAAA
AACTTGATTA
GGGTGATGGT
TCACGTAGTG
GGCCATCGCC
CTGATAGACG
GTTTTTCGCC
CTTTGACGTT
GGAGTCCACG
TTCTTTAATA
GTGGACTCTT
GTTCCAAACT
GGAACAACAC
TCAACCCTAT
CTCGGTCTAT
TCTTTTGATT
TATAAGGGAT
TTTGGGGATT
TCGGCCTATT
GGTTAAAAAA
TGAGCTGATT
TAACAAAAAT
TTAACGCGAA
TTAATTCTGT
GGAATGTGTG
TCAGTTAGGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCTGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAAAGCT
CCCGGGAGCT
TGTATATCCA
TTTTCGGATC
TGATCAAGAG
ACAGGATGAG
GATCGTTTCG
CATGATTGAA
CAAGATGGAT
TGCACGCAGG
TTCTCCGGCC
GCTTGGGTGG
AGAGGCTATT
CGGCTATGAC
TGGGCACAAC
AGACAATCGG
CTGCTCTGAT
GCCGCCGTGT
TCCGGCTGTC
AGCGCAGGGG
CGCCCGGTTC
TTTTTGTCAA
GACCGACCTG
TCCGGTGCCC
TGAATGAACT
GCAGGACGAG
GCAGCGCGGC
TATCGTGGCT
GGCCACGACG
GGCGTTCCTT
GCGCAGCTGT
GCTCGACGTT
GTCACTGAAG
CGGGAAGGGA
CTGGCTGCTA
TTGGGCGAAG
TGCCGGGGCA
GGATCTCCTG
TCATCTCACC
TTGCTCCTGC
CGAGAAAGTA
TCCATCATGG
CTGATGCAAT
GCGGCGGCTG
CATACGCTTG
ATCCGGCTAC
CTGCCCATTC
GACCACCAAG
CGAAACATCG
CATCGAGCGA
GCACGTACTC
GGATGGAAGC
CGGTCTTGTC
GATCAGGATG
ATCTGGACGA
AGAGCATCAG
GGGCTCGCGC
CAGCCGAACT
GTTCGCCAGG
CTCAAGGCGC
GCATGCCCGA
CGGCGAGGAT
CTCGTCGTGA
CCCATGGCGA
TGCCTGCTTG
CCGAATATCA
TGGTGGAAAA
TGGCCGCTTT
TCTGGATTCA
TCGACTGTGG
CCGGCTGGGT
GTGGCGGACC
GCTATCAGGA
CATAGCGTTG
GCTACCCGTG
ATATTGCTGA
AGAGCTTGGC
GGCGAATGGG
CTGACCGCTT
CCTCGTGCTT
TACGGTATCG
CCGCTCCCGA
TTCGCAGCGC
ATCGCCTTCT
ATCGCCTTCT
TGACGAGTTC
TTCTGAGCGG
GACTCTGGGG
TTCGCGAAAT
GACCGACCAA
GCGACGCCCA
ACCTGCCATC
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCAAT
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCG
ACGGATCGGG
AGATCTCCCG
ATCCCCTATG
GTCGACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGTATC
TGCTCCCTGC
TTGTGTGTTG
GAGGTCGCTG
AGTAGTGCGC
GAGCAAAATT
TAAGCTACAA
CAAGGCAAGG
CTTGACCGAC
AATTGCATGA
AGAATCTGCT
TAGG