名称: | pLenti-CMV-TRIM8(human)-TagGFP2-Puro |
---|---|
别称: | NM_030912.3;GERP; RNF27 |
基因长: | 1653bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLenti-CMV-MCS-GFP-SV-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLenti-CMV-TRIM8(human)-TagGFP2-Puro是适用于哺乳细胞过表达的质粒。质粒携带的CMV启动子可启动人源TRIM8基因高效表达。质粒在TRIM8基因C端融合表达TagGFP2绿色荧光蛋白,便于直观地观察目的基因表达的位置和水平等。质粒携带Blast抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGAAGAGCG
CCCAATACGC
AAACCGCCTC
TCCCCGCGCG
TTGGCCGATT
CATTAATGCA
GCTGGCACGA
CAGGTTTCCC
GACTGGAAAG
CGGGCAGTGA
GCGCAACGCA
ATTAATGTGA
GTTAGCTCAC
TCATTAGGCA
CCCCAGGCTT
TACACTTTAT
GCTTCCGGCT
CGTATGTTGT
GTGGAATTGT
GAGCGGATAA
CAATTTCACA
CAGGAAACAG
CTATGACCAT
GATTACGCCA
AGCGCGCAAT
TAACCCTCAC
TAAAGGGAAC
AAAAGCTGGA
GCTGCAAGCT
TAATGTAGTC
TTATGCAATA
CTCTTGTAGT
CTTGCAACAT
GGTAACGATG
AGTTAGCAAC
ATGCCTTACA
AGGAGAGAAA
AAGCACCGTG
CATGCCGATT
GGTGGAAGTA
AGGTGGTACG
ATCGTGCCTT
ATTAGGAAGG
CAACAGACGG
GTCTGACATG
GATTGGACGA
ACCACTGAAT
TGCCGCATTG
CAGAGATATT
GTATTTAAGT
GCCTAGCTCG
ATACATAAAC
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTGGCGCCC
GAACAGGGAC
TTGAAAGCGA
AAGGGAAACC
AGAGGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCGCTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCG
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTTGG
AATCACACGA
CCTGGATGGA
GTGGGACAGA
GAAATTAACA
ATTACACAAG
CTTAATACAC
TCCTTAATTG
AAGAATCGCA
AAACCAGCAA
GAAAAGAATG
AACAAGAATT
ATTGGAATTA
GATAAATGGG
CAAGTTTGTG
GAATTGGTTT
AACATAACAA
ATTGGCTGTG
GTATATAAAA
TTATTCATAA
TGATAGTAGG
AGGCTTGGTA
GGTTTAAGAA
TAGTTTTTGC
TGTACTTTCT
ATAGTGAATA
GAGTTAGGCA
GGGATATTCA
CCATTATCGT
TTCAGACCCA
CCTCCCAACC
CCGAGGGGAC
CCGACAGGCC
CGAAGGAATA
GAAGAAGAAG
GTGGAGAGAG
AGACAGAGAC
AGATCCATTC
GATTAGTGAA
CGGATCTCGA
CGGTATCGGT
TAACTTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
TTCAAAATTT
TATCGATAAG
CTTGGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGAGC
TCGTTTAGTG
AACCGTCAGA
TCGCCTGGAG
ACGCCATCCA
CGCTGTTTTG
ACCTCCATAG
AAGACACCGA
CTCTAGAGCC
ACCATGGCGG
AGAATTGGAA
GAACTGCTTC
GAGGAGGAGC
TCATCTGCCC
TATCTGCCTG
CACGTTTTCG
TGGAGCCAGT
GCAGCTGCCG
TGCAAACACA
ACTTCTGCCG
GGGCTGCATC
GGCGAGGCGT
GGGCCAAGGA
CAGCGGCCTC
GTACGCTGCC
CAGAGTGCAA
CCAGGCCTAC
AACCAGAAGC
CGGGCCTGGA
GAAGAACCTG
AAGCTCACCA
ACATCGTGGA
GAAGTTCAAT
GCCCTGCACG
TGGAGAAGCC
GCCGGCGGCG
CTGCACTGCG
TGTTCTGCCG
CCGCGGCCCC
CCGCTGCCCG
CGCAGAAGGT
CTGCCTGCGC
TGCGAGGCGC
CCTGCTGCCA
GTCCCACGTG
CAGACGCACC
TGCAGCAGCC
CTCCACCGCC
CGCGGGCACC
TCCTGGTGGA
GGCGGACGAC
GTGCGGGCCT
GGAGCTGCCC
GCAGCACAAC
GCCTACCGCC
TCTACCACTG
CGAGGCCGAG
CAGGTGGCCG
TGTGCCAGTA
CTGCTGCTAC
TACAGCGGCG
CGCATCAGGG
ACACTCGGTG
TGCGACGTGG
AGATCCGAAG
GAATGAAATC
CGGAAGATGC
TCATGAAGCA
GCAGGACCGG
CTGGAGGAGC
GAGAGCAGGA
CATTGAGGAC
CAGCTGTACA
AACTCGAGTC
AGACAAGCGC
CTGGTGGAGG
AGAAAGTGAA
CCAACTGAAG
GAGGAAGTTC
GGCTGCAGTA
CGAGAAGCTG
CACCAGCTGC
TGGACGAGGA
CCTGCGGCAG
ACAGTGGAGG
TCCTAGACAA
GGCCCAGGCC
AAGTTCTGCA
GCGAGAACGC
AGCGCAGGCG
CTGCACCTCG
GGGAGCGCAT
GCAGGAGGCC
AAGAAGCTGC
TGGGCTCCCT
GCAGCTGCTC
TTTGATAAGA
CGGAGGATGT
CAGCTTCATG
AAGAACACCA
AGTCTGTGAA
AATCCTGATG
GACAGGACCC
AGACCTGCAC
GAGCAGCAGC
CTTTCCCCCA
CTAAGATCGG
CCACCTGAAC
TCCAAGCTCT
TCCTGAACGA
AGTGGCCAAG
AAGGAGAAGC
AGCTGCGGAA
AATGCTAGAA
GGCCCCTTCA
GCACGCCGGT
GCCCTTCCTG
CAGAGTGTCC
CCCTGTACCC
TTGCGGCGTG
AGCAGCTCTG
GGGCGGAAAA
GCGCAAGCAC
TCAACGGCCT
TCCCAGAGGC
CAGTTTCCTA
GAGACGTCGT
CGGGCCCTGT
GGGCGGCCAG
TACGGGGCGG
CGGGCACAGC
CAGCGGTGAG
GGCCAGTCTG
GGCAGCCCCT
GGGGCCCTGC
AGCTCCACGC
AGCACTTGGT
GGCCCTGCCG
GGCGGCGCCC
AACCAGTGCA
CTCAAGCCCC
GTGTTCCCCC
CATCGCAGTA
TCCCAATGGC
TCCGCCGCCC
AGCAGCCCAT
GCTCCCCCAG
TATGGCGGCC
GCAAGATTCT
CGTCTGTTCT
GTGGACAACT
GTTACTGTTC
TTCCGTGGCC
AACCATGGCG
GCCACCAGCC
CTACCCCCGC
TCCGGCCACT
TTCCCTGGAC
AGTGCCCTCG
CAGGAGTACT
CACACCCGCT
CCCGCCCACA
CCCTCCGTCC
CCCAGTCCCT
TCCCAGCCTG
GCGGTCAGAG
ACTGGCTTGA
CGCCTCCCAG
CAGCCCGGCC
ACCAGGATTT
CTACAGGGTG
TATGGGCAGC
CGTCCACCAA
ACACTACGTG
ACGAGCGGCG
GCGGAGGGAG
TGGAGGAGGG
GGAAGCATGA
GCGGGGGCGA
GGAGCTGTTC
GCCGGCATCG
TGCCCGTGCT
GATCGAGCTG
GACGGCGACG
TGCACGGCCA
CAAGTTCAGC
GTGCGCGGCG
AGGGCGAGGG
CGACGCCGAC
TACGGCAAGC
TGGAGATCAA
GTTCATCTGC
ACCACCGGCA
AGCTGCCCGT
GCCCTGGCCC
ACCCTGGTGA
CCACCCTCTG
CTACGGCATC
CAGTGCTTCG
CCCGCTACCC
CGAGCACATG
AAGATGAACG
ACTTCTTCAA
GAGCGCCATG
CCCGAGGGCT
ACATCCAGGA
GCGCACCATC
CAGTTCCAGG
ACGACGGCAA
GTACAAGACC
CGCGGCGAGG
TGAAGTTCGA
GGGCGACACC
CTGGTGAACC
GCATCGAGCT
GAAGGGCAAG
GACTTCAAGG
AGGACGGCAA
CATCCTGGGC
CACAAGCTGG
AGTACAGCTT
CAACAGCCAC
AACGTGTACA
TCCGCCCCGA
CAAGGCCAAC
AACGGCCTGG
AGGCTAACTT
CAAGACCCGC
CACAACATCG
AGGGCGGCGG
CGTGCAGCTG
GCCGACCACT
ACCAGACCAA
CGTGCCCCTG
GGCGACGGCC
CCGTGCTGAT
CCCCATCAAC
CACTACCTGA
GCACTCAGAC
CAAGATCAGC
AAGGACCGCA
ACGAGGCCCG
CGACCACATG
GTGCTCCTGG
AGTCCTTCAG
CGCCTGCTGC
CACACCCACG
GCATGGACGA
GCTGTACAGG
TCCGGACTCA
GATAAGGATC
CGGACTAGTT
AACGCGTCGA
CCAGTGGTCG
ACCCTGTGGA
ATGTGTGTCA
GTTAGGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCAGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAT
AGTCCCGCCC
CTAACTCCGC
CCATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CGGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GGCTTTTGCA
AAAAGCTTAC
CATGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCAGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGATCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGCGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
GTGCCCGAAG
GACCGCGCAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
ACGCCCGCCC
CACGACCCGC
AGCGCCCGAC
CGAAAGGAGC
GCACGACCCC
ATGCATCGCC
AGTCGACAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAGCTGACGT
CCTTTCCATG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCCTGG
AATTCGAGCT
CGGTACCTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ACAAGATCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTAG
TAGTTCATGT
CATCTTATTA
TTCAGTATTT
ATAACTTGCA
AAGAAATGAA
TATCAGAGAG
TGAGAGGAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGC
TCTAGCTATC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGGA
CGTACCCAAT
TCGCCCTATA
GTGAGTCGTA
TTACGCGCGC
TCACTGGCCG
TCGTTTTACA
ACGTCGTGAC
TGGGAAAACC
CTGGCGTTAC
CCAACTTAAT
CGCCTTGCAG
CACATCCCCC
TTTCGCCAGC
TGGCGTAATA
GCGAAGAGGC
CCGCACCGAT
CGCCCTTCCC
AACAGTTGCG
CAGCCTGAAT
GGCGAATGGG
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTT
TAACAAAATA
TTAACGCTTA
CAATTTAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTTCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
G