名称: | pLV2-CMV-TRPA1(human)-Puro |
---|---|
别称: | NM_007332.3;ANKTM1; FEPS; FEPS1; p120; |
基因长: | 3360bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLVX-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV2-CMV-TRPA1(human)-Puro是适用于哺乳细胞过表达的质粒。CMV强启动子启动人源TRPA1基因的转录。质粒在TRPA1基因C端融合表达3×Myc标签,便于抗-Myc抗体在免疫印迹、免疫沉淀或免疫荧光等实验中识别和追踪带有Myc标签的目的蛋白,从而研究目的蛋白的表达水平、亚细胞定位以及与其他蛋白的相互作用等。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATAAGCTT
GGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGACTC
TACTAGAGGA
TCGCTAGCGC
TACCGGACTC
AGATCTCGAG
CTCAAGCTTC
GAATTCGCCA
CCATGAAGCG
CAGCCTGAGG
AAGATGTGGC
GCCCTGGAGA
AAAGAAGGAG
CCCCAGGGCG
TTGTCTATGA
GGATGTGCCG
GACGACACGG
AGGATTTCAA
GGAATCGCTT
AAGGTGGTTT
TTGAAGGAAG
TGCATATGGA
TTACAAAACT
TTAATAAGCA
AAAGAAATTA
AAAAGATGTG
ACGATATGGA
CACCTTCTTC
TTGCATTATG
CTGCAGCAGA
AGGCCAAATT
GAGCTAATGG
AGAAGATCAC
CAGAGATTCC
TCTTTGGAAG
TGCTGCATGA
AATGGATGAT
TATGGAAATA
CCCCTCTGCA
TTGTGCTGTA
GAAAAAAACC
AAATTGAAAG
CGTTAAGTTT
CTTCTCAGCA
GAGGAGCAAA
CCCAAATCTC
CGAAACTTCA
ACATGATGGC
TCCTCTCCAC
ATAGCTGTGC
AGGGCATGAA
TAATGAGGTG
ATGAAGGTCT
TGCTTGAGCA
TAGAACTATT
GATGTTAATT
TGGAAGGAGA
AAATGGAAAC
ACAGCTGTGA
TCATTGCGTG
CACCACAAAT
AATAGCGAAG
CATTGCAGAT
TTTGCTTAAA
AAAGGAGCTA
AGCCATGTAA
ATCAAATAAA
TGGGGATGTT
TCCCTATTCA
CCAAGCTGCA
TTTTCAGGTT
CCAAAGAATG
CATGGAAATA
ATACTAAGGT
TTGGTGAAGA
GCATGGGTAC
AGTAGACAGT
TGCACATTAA
CTTTATGAAT
AATGGGAAAG
CCACCCCTCT
CCACCTGGCT
GTGCAAAATG
GTGACTTGGA
AATGATCAAA
ATGTGCCTGG
ACAATGGTGC
ACAAATAGAC
CCAGTGGAGA
AGGGAAGGTG
CACAGCCATT
CATTTTGCTG
CCACCCAGGG
AGCCACTGAG
ATTGTTAAAC
TGATGATATC
GTCCTATTCT
GGTAGCGTGG
ATATTGTTAA
CACAACCGAT
GGATGTCATG
AGACCATGCT
TCACAGAGCT
TCATTGTTTG
ATCACCATGA
GCTAGCAGAC
TATTTAATTT
CAGTGGGAGC
AGATATTAAT
AAGATCGATT
CTGAAGGACG
CTCTCCACTT
ATATTAGCAA
CTGCTTCTGC
ATCTTGGAAT
ATTGTAAATT
TGCTACTCTC
TAAAGGTGCC
CAAGTAGACA
TAAAAGATAA
TTTTGGACGT
AATTTTCTGC
ATTTAACTGT
ACAGCAACCT
TATGGATTAA
AAAATCTGCG
ACCTGAATTT
ATGCAGATGC
AACAGATCAA
AGAGCTGGTA
ATGGATGAAG
ACAACGATGG
GTGTACTCCT
CTACATTATG
CATGTAGACA
GGGGGGCCCT
GGTTCTGTAA
ATAACCTACT
TGGCTTTAAT
GTGTCCATTC
ATTCCAAAAG
CAAAGATAAG
AAATCACCTC
TGCATTTTGC
AGCCAGTTAT
GGGCGTATCA
ATACCTGTCA
GAGGCTCCTA
CAAGACATAA
GTGATACGAG
GCTTCTGAAT
GAAGGTGACC
TTCATGGAAT
GACTCCTCTC
CATCTGGCAG
CAAAGAATGG
ACATGATAAA
GTAGTTCAGC
TTCTTCTGAA
AAAAGGTGCA
TTGTTTCTCA
GTGACCACAA
TGGCTGGACA
GCTTTGCATC
ATGCGTCCAT
GGGCGGGTAC
ACTCAGACCA
TGAAGGTCAT
TCTTGATACT
AATTTGAAGT
GCACAGATCG
CCTGGATGAA
GACGGGAACA
CTGCACTTCA
CTTTGCTGCA
AGGGAAGGCC
ACGCCAAAGC
CGTTGCGCTT
CTTCTGAGCC
ACAATGCTGA
CATAGTCCTG
AACAAGCAGC
AGGCCTCCTT
TTTGCACCTT
GCACTTCACA
ATAAGAGGAA
GGAGGTTGTT
CTTACGATCA
TCAGGAGCAA
AAGATGGGAT
GAATGTCTTA
AGATTTTCAG
TCATAATTCT
CCAGGCAATA
AATGTCCAAT
TACAGAAATG
ATAGAATACC
TCCCTGAATG
CATGAAGGTA
CTTTTAGATT
TCTGCATGTT
GCATTCCACA
GAAGACAAGT
CCTGCCGAGA
CTATTATATC
GAGTATAATT
TCAAATATCT
TCAATGTCCA
TTAGAATTCA
CCAAAAAAAC
ACCTACACAG
GATGTTATAT
ATGAACCGCT
TACAGCCCTC
AACGCAATGG
TACAAAATAA
CCGCATAGAG
CTTCTCAATC
ATCCTGTGTG
TAAAGAATAT
TTACTCATGA
AATGGTTGGC
TTATGGATTT
AGAGCTCATA
TGATGAATTT
AGGATCTTAC
TGTCTTGGTC
TCATACCTAT
GACCATTCTC
GTTGTCAATA
TAAAACCAGG
AATGGCTTTC
AACTCAACTG
GCATCATCAA
TGAAACTAGT
GATCATTCAG
AAATACTAGA
TACCACGAAT
TCATATCTAA
TAAAAACTTG
TATGATTTTA
GTGTTTTTAT
CAAGTATATT
TGGGTATTGC
AAAGAAGCGG
GGCAAATTTT
CCAACAGAAA
AGGAATTATT
TTATGGATAT
AAGCAATGTT
CTTGAATGGA
TTATCTACAC
GACGGGCATC
ATTTTTGTGC
TGCCCTTGTT
TGTTGAAATA
CCAGCTCATC
TGCAGTGGCA
ATGTGGAGCA
ATTGCTGTTT
ACTTCTATTG
GATGAATTTC
TTATTGTATC
TTCAAAGATT
TGAAAATTGT
GGAATTTTTA
TTGTTATGTT
GGAGGTAATT
TTGAAAACTT
TGTTGAGGTC
TACAGTTGTA
TTTATCTTCC
TTCTTCTGGC
TTTTGGACTC
AGCTTTTACA
TCCTCCTGAA
TTTACAGGAT
CCCTTCAGCT
CTCCATTGCT
TTCTATAATC
CAGACCTTCA
GCATGATGCT
AGGAGATATC
AATTATCGAG
AGTCCTTCCT
AGAACCATAT
CTGAGAAATG
AATTGGCACA
TCCAGTTCTG
TCCTTTGCAC
AACTTGTTTC
CTTCACAATA
TTTGTCCCAA
TTGTCCTCAT
GAATTTACTT
ATTGGTTTGG
CAGTTGGCGA
CATTGCTGAG
GTCCAGAAAC
ATGCATCATT
GAAGAGGATA
GCTATGCAGG
TGGAACTTCA
TACCAGCTTA
GAGAAGAAGC
TGCCACTTTG
GTTTCTACGC
AAAGTGGATC
AGAAATCCAC
CATCGTGTAT
CCCAACAAAC
CCAGATCTGG
TGGGATGTTA
TTCCATATAT
TCTGTTTTTT
ATTTTGCACT
GGGGAAATAA
GACAAGAAAT
ACCAAATGCT
GATAAATCTT
TAGAAATGGA
AATATTAAAG
CAGAAATACC
GGCTGAAGGA
TCTTACTTTT
CTCCTGGAAA
AACAGCATGA
GCTCATTAAA
CTGATCATTC
AGAAGATGGA
GATCATCTCT
GAGACAGAGG
ATGATGATAG
CCATTGTTCT
TTTCAAGACA
GGTTTAAGAA
AGAGCAGATG
GAACAAAGGA
ATAGCAGATG
GAATACTGTG
TTGAGAGCAG
TCAAGGCAAA
AACACACCAT
CTTGAGCCTT
AGGGATCCCG
CGACTCTAGA
TAATTCTACC
GGGTAGGGGA
GGCGCTTTTC
CCAAGGCAGT
CTGGAGCATG
CGCTTTAGCA
GCCCCGCTGG
GCACTTGGCG
CTACACAAGT
GGCCTCTGGC
CTCGCACACA
TTCCACATCC
ACCGGTAGGC
GCCAACCGGC
TCCGTTCTTT
GGTGGCCCCT
TCGCGCCACC
TTCTACTCCT
CCCCTAGTCA
GGAAGTTCCC
CCCCGCCCCG
CAGCTCGCGT
CGTGCAGGAC
GTGACAAATG
GAAGTAGCAC
GTCTCACTAG
TCTCGTGCAG
ATGGACAGCA
CCGCTGAGCA
ATGGAAGCGG
GTAGGCCTTT
GGGGCAGCGG
CCAATAGCAG
CTTTGCTCCT
TCGCTTTCTG
GGCTCAGAGG
CTGGGAAGGG
GTGGGTCCGG
GGGCGGGCTC
AGGGGCGGGC
TCAGGGGCGG
GGCGGGCGCC
CGAAGGTCCT
CCGGAGGCCC
GGCATTCTGC
ACGCTTCAAA
AGCGCACGTC
TGCCGCGCTG
TTCTCCTCTT
CCTCATCTCC
GGGCCTTTCG
ACCTGCAGCC
CAAGCTTACC
ATGACCGAGT
ACAAGCCCAC
GGTGCGCCTC
GCCACCCGCG
ACGACGTCCC
CAGGGCCGTA
CGCACCCTCG
CCGCCGCGTT
CGCCGACTAC
CCCGCCACGC
GCCACACCGT
CGATCCGGAC
CGCCACATCG
AGCGGGTCAC
CGAGCTGCAA
GAACTCTTCC
TCACGCGCGT
CGGGCTCGAC
ATCGGCAAGG
TGTGGGTCGC
GGACGACGGC
GCCGCGGTGG
CGGTCTGGAC
CACGCCGGAG
AGCGTCGAAG
CGGGGGCGGT
GTTCGCCGAG
ATCGGCCCGC
GCATGGCCGA
GTTGAGCGGT
TCCCGGCTGG
CCGCGCAGCA
ACAGATGGAA
GGCCTCCTGG
CGCCGCACCG
GCCCAAGGAG
CCCGCGTGGT
TCCTGGCCAC
CGTCGGCGTC
TCGCCCGACC
ACCAGGGCAA
GGGTCTGGGC
AGCGCCGTCG
TGCTCCCCGG
AGTGGAGGCG
GCCGAGCGCG
CCGGGGTGCC
CGCCTTCCTG
GAGACCTCCG
CGCCCCGCAA
CCTCCCCTTC
TACGAGCGGC
TCGGCTTCAC
CGTCACCGCC
GACGTCGAGG
TGCCCGAAGG
ACCGCGCACC
TGGTGCATGA
CCCGCAAGCC
CGGTGCCTGA
CCGCGTCTGG
AACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAGC
TGACGTCCTT
TCCATGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCCTGGAATT
AATTCTGCAG
TCGAGACCTA
GAAAAACATG
GAGCAATCAC
AAGTAGCAAT
ACAGCAGCTA
CCAATGCTGA
TTGTGCCTGG
CTAGAAGCAC
AAGAGGAGGA
GGAGGTGGGT
TTTCCAGTCA
CACCTCAGGT
ACCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GAGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGACAA
GATATCCTTG
ATCTGTGGAT
CTACCACACA
CAAGGCTACT
TCCCTGATTA
GCAGAACTAC
ACACCAGGGC
CAGGGGTCAG
ATATCCACTG
ACCTTTGGAT
GGTGCTACAA
GCTAGTACCA
GTTGAGCCAG
ATAAGGTAGA
AGAGGCCAAT
AAAGGAGAGA
ACACCAGCTT
GTTACACCCT
GTGAGCCTGC
ATGGGATGGA
TGACCCGGAG
AGAGAAGTGT
TAGAGTGGAG
GTTTGACAGC
CGCCTAGCAT
TTCATCACGT
GGCCCGAGAG
CTGCATCCGG
AGTACTTCAA
GAACTGCTGA
TATCGAGCTT
GCTACAAGGG
ACTTTCCGCT
GGGGACTTTC
CAGGGAGGCG
TGGCCTGGGC
GGGACTGGGG
AGTGGCGAGC
CCTCAGATCC
TGCATATAAG
CAGCTGCTTT
TTGCCTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTAGTAGT
TCATGTCATC
TTATTATTCA
GTATTTATAA
CTTGCAAAGA
AATGAATATC
AGAGAGTGAG
AGGCCTTGAC
ATTGCTAGCG
TTTTACCGTC
GACCTCTAGC
TAGAGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTCGA
CGGATCGGGA
GATCAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGATCA
ACTGGATAAC
TCAAGCTAAC
CAAAATCATC
CCAAACTTCC
CACCCCATAC
CCTATTACCA
CTGCCAATTA
CCTGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGTTTTT
AAAGAAATTG
TATTTGTTAA
ATATGTACTA
CAAACTTAGT
AGT