名称: | pCMV-Trps1(mouse)-3×FLAG-Neo |
---|---|
别称: | NM_001310481.1;D15Ertd586e; |
基因长: | 3855bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-T7-MCS-3×FLAG-WPRE-Neo |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pCMV-Trps1(mouse)-3×FLAG-Neo是适用于哺乳细胞过表达的质粒。质粒带有的CMV增强子和CMV启动子可联合启动小鼠源Trps1基因高效表达,促进Trps1基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。WPRE蛋白可增强目的基因转录后的加工和稳定性,有助于增加mRNA的半衰期和翻译效率。质粒携带Neo/G418抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTAGGCGTT
TTGCGCTGCT
TCGCGATGTA
CGGGCCAGAT
ATACGCGTTG
ACATTGATTA
TTGACTAGTT
ATTAATAGTA
ATCAATTACG
GGGTCATTAG
TTCATAGCCC
ATATATGGAG
TTCCGCGTTA
CATAACTTAC
GGTAAATGGC
CCGCCTGGCT
GACCGCCCAA
CGACCCCCGC
CCATTGACGT
CAATAATGAC
GTATGTTCCC
ATAGTAACGC
CAATAGGGAC
TTTCCATTGA
CGTCAATGGG
TGGAGTATTT
ACGGTAAACT
GCCCACTTGG
CAGTACATCA
AGTGTATCAT
ATGCCAAGTA
CGCCCCCTAT
TGACGTCAAT
GACGGTAAAT
GGCCCGCCTG
GCATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTCTA
TATAAGCAGA
GCTCTCTGGC
TAACTAGAGA
ACCCACTGCT
TACTGGCTTA
TCGAAATTAA
TACGACTCAC
TATAGGGAGA
CCCAAGCTGG
CTAGCGTTTA
AACTTAAGCT
TGGTACCGAG
CTCGAATTCG
CCACCATGCA
GAGTAATATG
GTCCGGAAAA
AGAACCCCCC
TCTGAGAAAC
GTTGCTAGTG
AAGGCGAGGG
TCAAACCCTG
GAGCCAACAG
CTACAGAGAG
CAAGGTATCT
GGGAAGAACA
AAGAATTGTC
TGCAGATCAG
ATGTCAGAAA
ATACAGATCA
GAGTGATGTT
GCAGAGTTGA
ACTCGAAGGA
GGAACACAGC
ACGCATGGCC
AAGAGCCATC
TTCCAGCGGT
AAGAAGGACT
TGCAAATCTC
AGGCCTGAGT
GAGAAGGCTG
GCTTCAATTA
TGAAAGCCCC
AGTAAGGGAG
GGAGCCTTGT
CTCCTTCCCA
CATGATGAGG
TAACAGACAG
AAATATGTTG
GCTTTCTCAT
CTCCAGCTGC
TGGGGGAGTC
TGCGAGCCCT
TGAAGTCTCC
TCAAAGAGCA
GAGGCAGATG
ACCCTCAAGA
TATGGCCTGC
ACCCCATCAG
GGGATTCATT
GGAGACAAAG
GAAGAGCATA
AAATGTCACC
AAAGGCCACT
GAGGAAACAG
GGCCAGTGCA
GAGTGGTCAA
GCCAATTGTC
AAGGTTTGAG
TCCAGTTTCC
GTGGCCTCAA
AAAACCCACA
AGTGCCTTCA
GATGGGGGTG
TCAGACTGAG
TAAACCCAAA
GGTGACTTAC
TGGTGAATGA
CAACCCAGAC
CCGGCACCTC
TGTCTCCAGA
GCTTCAGGAC
TTTAAATGCA
ATATCTGTGG
ATACGGTTAC
TATGGCAATG
ACCCCACAGA
TCTGATTAAG
CACTTCCGAA
AGTATCACTT
AGGACTGCAT
AATCGCACCA
GGCAGGATGC
TGAGCTGGAC
AGCAAAATCT
TGGCCCTTCA
TAACATGGTG
CAGTTCAGCC
ATTCCAAAGA
CTTCCAGAAG
GTCAACCGTT
CTGTGCTTTC
TGGCGTGCTG
CAGGATATCA
GCTCTTCACG
GCCTGCTTTA
CTAAACGGAA
CCTATGATGT
ACAGGTCACT
TCAGGAGGAA
CTTTCATTGG
CATTGGACGG
AAAACTCCAG
ATTGCCAAGG
CAACACCAAG
TATTTCCGCT
GCAAATTCTG
CAATTTCACT
TATATGGGAA
ACTCCTCAAC
TGAACTAGAG
CAACATTTTC
TTCAGACGCA
CCCGAATAAA
ATCAAAGTGT
CTCTCCCGTC
CTCCGAGGGT
GTGAAACCTT
CAGAGAAAAA
CTCTAACAAA
TCCATCCCTG
CGCTTCGAGC
CAGTGACTCT
GGGGATGTGG
GAAAGTGGCA
GGACAAAATG
ACAGTCAAAG
CTGGAGATGA
CACCCCTGTC
GGCTACTCAG
TGCCCATCAA
GCCCCTCGAT
TCCTCACGAC
AAAATGGTAC
AGAGGCCACC
AGTTATTACT
GGTGTAAATT
TTGTAGTTTC
AGCTGTGAGT
CATCCAGCTC
CCTTAAGCTG
CTAGAACATT
ATGGCAAGCA
GCACGGAGCA
GTGCAGTCGG
GCGGCCTTAA
TCCAGAGTTG
AATGACAAGC
TTCCCAGGGG
CTCTGTCATT
AATCAGAATG
ATCTAGCCAA
AAGTGTAGAA
GGAGAGCCAT
TGACCAAGCC
AGAAAAGGGC
TTGAGTGGGG
CTAAAAAGAA
GGACTTCCCC
AGCAAGGGAG
CAGAGGATAA
TATGGTAACG
AGCTATAACT
GTCAGTTCTG
TGACTTTAGA
TATTCCAAAA
GCCATGGCCC
TGATGTGATT
GTAGTGGGTC
CACTTCTCCG
TCATTATCAG
CAACTCCATA
ACATCCACAA
GTGTACCATT
AAGCACTGTC
CATTCTGTCC
CAGAGGCCTT
TGCAGCCCAG
AAAAGCACCT
TGGAGAGATT
ACTTATCCGT
TTGCTTGTAG
AAAAAGTAAT
TGTTCCCACT
GTGCACTGTT
GCTGTTGCAC
TTGTCTCCTG
GGGTGGCCGG
AAGTTCGAGA
GTCAAACACC
AATGCCACCA
GTGTTCCTTC
TCTACCCCCG
ATGTAGATGT
GCTCCTCTTT
CACTATGAGA
CCGTGCATGA
GTCCCAAGCA
TCGGATGTCA
AACAGGAGGC
CAACCACCTG
CTAGGATCTG
ACGGGCAGCA
GGCTGTCAGG
GACAGCAAGG
AGCACTCGTG
CACCAAATGC
GATTTTATAA
CCCAGGTGGA
AGAAGAGATC
TCGAGACACT
ACAGGAGAGC
ACACAGCTGC
TATAAATGTC
GTCAGTGCAG
TTTCACAGCT
GCTGACACTC
AGTCACTATT
GGAGCACTTC
AACACAGTTC
ACTGCCAGGA
ACAGGAAATC
ACTACTGCTA
ATGGCGAAGA
GGGTGGCCAT
GCCATACCCA
CCATCAAAGA
AGAGCCCAAA
ATTGACCTCA
AGGTCTACAG
TCTGCTAAAC
CCAGACTCTA
AAATGGGAGA
AACAGTTCCC
GAGAGCATAG
TCAAGAGAGA
GAAACTTGAT
GACAAGGAAG
GGCTGAAAGA
TAAAATCTGG
ACTGAGAGTT
CAACTGATGA
CCTTCGTGGT
GTGGCTTGGA
GAGGGGCAGA
CATCCTACGG
GGTAGTCCAT
CCTACACTCA
AGCAAGCTTA
GGGGTCTTGA
CGCCTGTGTC
CAGCTCCCAA
GAGCAGACAA
AGACTCTGAG
GGACAGCCCC
AATGTTGAAG
CTGCCCACCT
GGCACGACCT
ATGTATGGCT
TGGCAGTAGA
TACCAAGGGC
TTCCTGCAAG
GGGCTCCTGC
TGGCAGCGAG
AAGTCTGCAT
CTCTTACCCA
GCAGTATCCT
GCCTCCGGAG
AGAGCAAGAC
CAAGGATGAA
TCCCAGTCGC
TGTTACGGAG
GCGAAGAGGA
TCTGGTGTTT
TTTGTGCCAA
TTGCCTGACC
ACAAAGACCT
CTCTCTGGCG
AAAGAATGCA
AATGGCGGAT
ATGTATGCAA
TGCATGTGGT
CTCTACCAGA
AACTTCACTC
GACTCCCAGG
CCTTTAAACA
TCATTAAACA
AAACAACGGC
GAGCAGATTA
TTAGAAGACG
GACAAGAAAG
CGCCTTAACC
CAGAGGCACT
TCAGGCTGAG
CAGCTCAACA
AACAGCAGAG
GGGAAGTGGG
GAGGAGCAGG
TCAATGGAAG
CCCCTTAGAG
AGGAGGTCAG
AAGATCACTT
AAGTGAAAGT
CATCCGAGAG
AAATTCCACT
TCCGAGCCTG
AGTAAATACG
AAGCCCAGGG
TTCATTGACT
AAAAGCCATT
CTGCTCAGCA
GCCAGTCCTG
GTCAGCCAAG
CTCTGGATAT
CCACAAAAGG
ATGCAGCCTT
TGCACATTCA
GATAAAAAGT
CCTCAGGAAA
GTACTGGAGA
TCCAGGAAAT
AGTTCATCCG
TATCTGATGG
GAAAGGAAGT
TCTGAAAGAG
GCAGCCCCAT
CGAAAAGTAC
ATGAGACCTG
CAAAACACCC
CAACTATTCT
CCACCAGGCA
GCCCCATCGA
AAAGTACCAA
TACCCGCTTT
TTGGAGTTCC
TTTTGTACAT
AATGACTTCC
AGAGTGAAGC
TGATTGGCTA
CGGTTCTGGA
GTAAATATAA
GCTCTCCGTT
CCTGGGAATC
CGCACTACTT
GAGTCATGTG
CCTGGCTTAC
CAAATCCTTG
CCAAAACTAT
GTGCCTTATC
CCACCTTCAA
TCTGCCTCCT
CATTTCTCAG
CTGTTGGATC
AGACAATGAC
ATTCCTCTAG
ATTTGGCGAT
CAAGCATTCC
AGACCTGGGC
CAACTGCAAA
TGGTGCCTCC
AAGGAGAAAA
CCAAGGCACC
ACCAACTGTA
AAAAATGAAG
GTCCCTTGAA
TGTAGTTAAG
ACGGAGAAAG
TTGACAGAAG
CACTCAAGAT
GAACTTTCAA
CGAAATGTGT
GCACTGTGGC
ATTGTTTTTC
TGGATGAAGT
GATGTATGCT
TTGCATATGA
GTTGCCATGG
TGACAGTGGA
CCTTTCCAGT
GCAGCATATG
CCAGCATCTT
TGCACGGACA
AATATGACTT
CACAACACAT
ATCCAGAGGG
GCCTGCATAG
GAACAATGCA
CAAGCAGAAA
AAAATGGAAA
ACCTAAAGAG
GGATCCGACT
ACAAAGACCA
TGACGGTGAT
TATAAAGATC
ATGACATCGA
CTACAAGGAT
GACGATGACA
AGTAGTGAGG
GCCCGATATC
TCGACAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
GCTGACGTCC
TTTCCATGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCCTGGAA
ACGGGGGAGG
CTAACTGAAA
CACGGAAGGA
GACAATACCG
GAAGGAACCC
GCGCTATGAC
GGCAATAAAA
AGACAGAATA
AAACGCACGG
GTGTTGGGTC
GTTTGTTCAT
AAACGCGGGG
TTCGGTCCCA
GGGCTGGCAC
TCTGTCGATA
CCCCACCGAG
ACCCCATTGG
GGCCAATACG
CCCGCGTTTC
TTCCTTTTCC
CCACCCCACC
CCCCAAGTTC
GGGTGAAGGC
CCAGGGCTCG
CAGCCAACGT
CGGGGCGGCA
GGCCCTGCCA
TAGCAGATCT
GCGCAGCTGG
GGCTCTAGGG
GGTATCCCCA
CGCGCCCTGT
AGCGGCGCAT
TAAGCGCGGC
GGGTGTGGTG
GTTACGCGCA
GCGTGACCGC
TACACTTGCC
AGCGCCCTAG
CGCCCGCTCC
TTTCGCTTTC
TTCCCTTCCT
TTCTCGCCAC
GTTCGCCGGC
TTTCCCCGTC
AAGCTCTAAA
TCGGGGCATC
CCTTTAGGGT
TCCGATTTAG
TGCTTTACGG
CACCTCGACC
CCAAAAAACT
TGATTAGGGT
GATGGTTCAC
GTAGTGGGCC
ATCGCCCTGA
TAGACGGTTT
TTCGCCCTTT
GACGTTGGAG
TCCACGTTCT
TTAATAGTGG
ACTCTTGTTC
CAAACTGGAA
CAACACTCAA
CCCTATCTCG
GTCTATTCTT
TTGATTTATA
AGGGATTTTG
GGGATTTCGG
CCTATTGGTT
AAAAAATGAG
CTGATTTAAC
AAAAATTTAA
CGCGAATTAA
TTCTGTGGAA
TGTGTGTCAG
TTAGGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCAGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCATA
GTCCCGCCCC
TAACTCCGCC
CATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
TGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
GCTTTTGCAA
AAAGCTCCCG
GGAGCTTGTA
TATCCATTTT
CGGATCTGAT
CAAGAGACAG
GATGAGGATC
GTTTCGCATG
ATTGAACAAG
ATGGATTGCA
CGCAGGTTCT
CCGGCCGCTT
GGGTGGAGAG
GCTATTCGGC
TATGACTGGG
CACAACAGAC
AATCGGCTGC
TCTGATGCCG
CCGTGTTCCG
GCTGTCAGCG
CAGGGGCGCC
CGGTTCTTTT
TGTCAAGACC
GACCTGTCCG
GTGCCCTGAA
TGAACTGCAG
GACGAGGCAG
CGCGGCTATC
GTGGCTGGCC
ACGACGGGCG
TTCCTTGCGC
AGCTGTGCTC
GACGTTGTCA
CTGAAGCGGG
AAGGGACTGG
CTGCTATTGG
GCGAAGTGCC
GGGGCAGGAT
CTCCTGTCAT
CTCACCTTGC
TCCTGCCGAG
AAAGTATCCA
TCATGGCTGA
TGCAATGCGG
CGGCTGCATA
CGCTTGATCC
GGCTACCTGC
CCATTCGACC
ACCAAGCGAA
ACATCGCATC
GAGCGAGCAC
GTACTCGGAT
GGAAGCCGGT
CTTGTCGATC
AGGATGATCT
GGACGAAGAG
CATCAGGGGC
TCGCGCCAGC
CGAACTGTTC
GCCAGGCTCA
AGGCGCGCAT
GCCCGACGGC
GAGGATCTCG
TCGTGACCCA
TGGCGATGCC
TGCTTGCCGA
ATATCATGGT
GGAAAATGGC
CGCTTTTCTG
GATTCATCGA
CTGTGGCCGG
CTGGGTGTGG
CGGACCGCTA
TCAGGACATA
GCGTTGGCTA
CCCGTGATAT
TGCTGAAGAG
CTTGGCGGCG
AATGGGCTGA
CCGCTTCCTC
GTGCTTTACG
GTATCGCCGC
TCCCGATTCG
CAGCGCATCG
CCTTCTATCG
CCTTCTTGAC
GAGTTCTTCT
GAGCGGGACT
CTGGGGTTCG
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
GATTTCGATT
CCACCGCCGC
CTTCTATGAA
AGGTTGGGCT
TCGGAATCGT
TTTCCGGGAC
GCCGGCTGGA
TGATCCTCCA
GCGCGGGGAT
CTCATGCTGG
AGTTCTTCGC
CCACCCCAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGTA
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCAATGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
GACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTCGACGG
ATCGGGAGAT
CTCCCGATCC
CCTATGGTCG
ACTCTCAGTA
CAATCTGCTC
TGATGCCGCA
TAGTTAAGCC
AGTATCTGCT
CCCTGCTTGT
GTGTTGGAGG
TCGCTGAGTA
GTGCGCGAGC
AAAATTTAAG
CTACAACAAG
GCAAGGCTTG
ACCGACAATT
GCATGAAGAA
TCTGCTTAGG