名称: | pLV3-GFAP(mouse)-Promoter-Kdm6b(mouse)-EF1a-CopGFP |
---|---|
别称: | NM_001017426.2;1700064E03Rik; Jmjd3 |
基因长: | 4923bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | GFAP |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pCDH-CMV-MCS-EF1-copGFP |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-GFAP(mouse)-Promoter-Kdm6b(mouse)-EF1a-CopGFP是适用于哺乳细胞蛋白表达的质粒。质粒携带的GFAP启动子能启动下游小鼠源Kdm6b基因在星形胶质细胞中特异性表达。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。携带的CopGFP绿色荧光蛋白EF1a启动子启动转录,用来检测目的基因的表达和定位情况
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTTAACTTT
TAAAAGAAAA
GGGGGGATTG
GGGGGTACAG
TGCAGGGGAA
AGAATAGTAG
ACATAATAGC
AACAGACATA
CAAACTAAAG
AATTACAAAA
ACAAATTACA
AAAATTCAAA
ATTTTATCGA
TACTAGTATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGCAGGAA
GACGGGGTGT
TACAGAAAGA
AAGACTCCAG
CATGCACAGC
CAACTCATTC
AAAACTACTC
TGTCAGGGGC
TGCCAGGGGC
CAGGCTCGGG
GTGGGGGGTG
GGGGGCAACG
AGAAGCTGGA
TCAGGGAGAA
ATGGCCCACT
AGGCTGGATA
AGAGGCCACA
GAGGGGCTCA
GGAATGAAGC
CTGCTGTCTT
ACCCTATTAG
GATCTGCGTG
CATACCTTCT
GCCGTGCACT
CTAAACACAC
AGCCAGAGGC
TCAAGTTGAC
CCAGGAGTCA
CAGAGAGGGC
TCCAACCTTA
GCCCTCCACT
CCTGAACTCC
AGGAATGAGA
AGATAGAGTT
GGAGAGATTC
AGGGGAGAGG
ACTCTGTTGA
GAATGGGGGT
CACAGGAAAC
TGTAATATAG
GTTGATCCCG
GAGGAAGGGA
ATAGGTTCTT
CAAGTTCCTA
GCATCTCACA
GGCCCCCAGA
GAAGGACAGA
GTTGGGGTGG
TCCTGGCTTA
CAGGCTCTAA
GAACTGGAAG
CTGATTACCC
CACCGAGCTG
TGCACTCTCT
GTCTCTGTCT
CTGTGTGTGC
GCTCGTGCAC
ACTTATCACA
CAAATGTTCA
TGTGTGTGCA
CATACATGTG
TTGAGACCAG
AGGTCAACCT
CAGGCACTGT
TGCCTTGGTT
TTCTGAGAGA
GCATTTCTCT
CTGGATCTGG
AACTCGCCAA
TTAGTGAGAG
CCAGGAAGTC
TGCTGATTTT
CACTGCCCAG
CACTGGAGTT
TACAAGTATG
CACTGTCAAC
CCAGGCCTTT
TGTATTCATT
CTGCAGCTAG
AACTTGGGTG
GGTCTTCATG
CTTGACAGGC
AAGCAATTTA
TGGACTAAGC
TGTTCCCTCG
GCCCTCTCTT
GACCCATTTA
CCAGAAAGGG
GGTTCCTTGA
TCAATGGCGA
AGCCAGGCTG
GTGTTCCCAA
GAAAGCCTTG
ACTCTGGGTA
CAGTGACCTC
AGTGGGGTGA
GAGGAGTTCT
CCCCCTAGCT
GGGCTGGGGC
CCAGCTCCAC
CCCCTCAGGC
TATTCAATGG
GGGTGCTTCC
AGGAAGTCAG
GGGCAGATTT
AGTCCAACCC
GTTCCTCCAT
AAAGGCCCTG
ACATCCCAGG
AGCCAGCAGA
GGCAGGGCAG
GTTGGAGCGG
AGACGCATCA
CCTCTGCGCG
CCGCTCCTTT
GCCTCCGAGA
CGGTGGTCAT
CGCTCGAGGA
GCTCGCGGCC
GCATAGGATC
CAGATCTAGA
TTCACCGGTC
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGGTA
CCGAGCTCGA
ATTCGCCACC
ATGCATCGGG
CAGTGGACCC
TCCAGGGGCC
CGCTCTGCAC
GGGAAGCCTT
TGCCCTTGGG
GGCTTGAGCT
GTGCTGGGGC
TTGGAGCTCC
TGCCCACCCC
ATCCTCCTCC
CCGAAGCTCA
TGGCTGCCCG
GAGGCAGATG
CTCTGCCAGC
GTTGGGCAGC
CCCCACTCTC
AGCTCCTTTA
CCCCCATCTC
ATGGCAGTAG
CTCCGGGCAC
CCTAACAAAC
CCTATTATGC
TCCTGGGACA
CCCACCCCAA
GACCCCTTCA
CGGGAAGTTG
GAATCCCTAC
ATGGCTGTGT
CCAGGCATTG
CTCCGGGAGC
CAGCGCAGCC
AGGGTTGTGG
GAACAGCTTG
GACAGTTGTA
TGAATCAGAG
CACGACAGTG
AGGAAGCCGT
ATGCTGCTAC
CATAGGGCCC
TTCGCTATGG
AGGAAGCTTC
GCCGAGCTGG
GACCCCGGAT
TGGCCGCTTG
CAGCAGGCCC
AGCTCTGGAA
CTTTCATGCC
GGTTCCTGTC
AGCACAGAGC
CAAGGTCCTG
CCTCCCCTGG
AGCAAGTCTG
GAATTTGCTG
CACCTTGAGC
ACAAACGGAA
CTATGGGGCT
AAGCGAGGGG
GCCCTCCAGT
GAAGAGATCT
GCTGAACCCC
CCGTGGTCCA
GCCTATGCCT
CCTGCAGCCC
TCTCAGGCCC
CTCAGGAGAG
GAGGGCCTTA
GCCCTGGAGG
CAAGCGCAGG
AGAGGCTGCA
GCTCTGAACA
GGCTGGCCTT
CCCCCAGGTC
TGCCACTCCC
TCCACCACCC
CCACCCCCAC
CGCCTCCACC
ACCACCACCA
CCCCCTCCAC
CACCACCGCT
GCCTGGCCTG
GCTATTAGCC
CCCCATTTCA
GCTGACTAAG
CCAGGGCTGT
GGAATACCCT
GCATGGAGAT
GCTTGGGGCC
CCGAGCGCAA
GGGTTCAGCG
CCGCCAGAGC
GCCAGGAGCA
GCGGCACTCG
ATGCCTCATT
CATATCCATA
CCCAGCTCCC
GCCTACTCCG
CTCATCCGCC
CAGCCATCGG
CTGGTCCCCA
ACACACCCCT
TGGTCCAGGT
CCCCGACCCC
CAGGAGCAGA
GAGCCATGGC
TGCCTGCCTG
CCACCCGTCC
CCCCGGAAGT
GACCTTAGAG
AGAGCAGAGT
TCAGAGGTCG
CGGATGGACT
CCAGCGTTTC
ACCAGCAGCA
TCTACCGCCT
GCGTGCCTTA
CGCCCCTTCC
CGGCCCCCTG
GCCTCCCCGG
CACCAGCAGC
AGCAGCAGCA
GCAGCAGTAG
CAGTAACAAC
ACTGGTCTTC
GGGGTGTGGA
GCCAAGCCCA
GGCATTCCTG
GCGCTGACCA
TTACCAAAAC
CCTGCGCTGG
AGATATCCCC
TCACCAGGCC
CGCCTGGGTC
CCTCCGCACA
CAGCAGTCGG
AAACCATTTT
TGACGGCCCC
TGCTGCCACG
CCCCACTTAT
CCCTACCCCC
TGGGACCCCA
TCATCCCCTC
CACCCCCATG
TCCTCGCCTC
TTGCGCCCTC
CACCGCCCCC
TGCTTGGATG
AAGGGCTCAG
CCTGCCGTGC
AGCCCGAGAG
GATGGAGAGA
TCTTAGGGGA
GCTCTTCTTT
GGTGCTGAGG
GACCTCCCCG
TCCTCCTCCC
CCACCCCTTC
CCCACCGTGA
TGGCTTCTTG
GGGCCTCCAA
ACCCCCGCTT
TTCTGTGGGC
ACTCAGGATT
CGCATAACCC
TCCCATTCCC
CCAACCACCA
CCAGCAGCAG
CAGCAGCAGC
AACAGCCACA
GCAGTAGTCC
TACTGGGCCG
GTGCCCTTTC
CACCACCCTC
CTATCTGGCC
AGAAGTATAG
ACCCCCTCCC
CAGGCCATCC
AGCCCAACCT
TGAGCCCCCA
GGACCCACCT
CTTCCACCAC
TGACTCTTGC
CCTGCCTCCA
GCCCCTCCCT
CCTCCTGCCA
CCAAAATACC
TCAGGAAGCT
TCAGGCGCTC
GGAGAGCCCC
CGGCCCAGGG
TCTCCTTCCC
AAAGACCCCC
GAGGTGGGGC
AGGGGCCACC
CCCAGGCCCT
GTGAGTAAAG
CCCCCCAGCC
TGTGCCACCT
GGGGTTGGAG
AGCTGCCTGC
CCGAGGCCCG
AGGCTCTTTG
ATTTCCCACC
CACTCCGCTG
GAGGACCAGT
TTGAAGAGCC
AGCCGAATTC
AAGATCCTAC
CTGATGGGCT
GGCAAACATC
ATGAAGATGC
TGGATGAATC
CATTCGGAAG
GAGGAGGAGC
AGCAGCAGCA
GCAGGAGGCA
GGCGTGGCTC
CCCCACCCCC
ACTCAAAGAG
CCCTTTGCAT
CTCTACAGCC
TCCATTTCCC
AGTGACACAG
CCCCAGCCAC
CACCACTGCT
GCCCCCACCA
CCGCCACCAC
CACCACAACC
ACCACCACCA
CCACCACCCA
AGAAGAGGAG
AAGAAGCCAC
CACCAGCCCT
ACCACCACCA
CCGCCTCTAG
CCAAGTTTCC
TCCACCTCCC
CAGCCACAGC
CCCCACCACC
TCCACCAGCC
AGCCCAGCCA
GCCTGCTCAA
ATCGTTGGCC
TCTGTTCTTG
AGGGACAAAA
GTACTGTTAC
CGGGGGACTG
GAGCAGCCGT
CTCAACCAGG
CCCGGGTCCG
TGCCCGCCAC
TCAGTATTCC
CCTAGTCCTG
CATCAGGTGC
TACCGCCCCA
CCACCCACTT
CAGTGGCCCC
TAGTGCCCAG
GGCTCCCCCA
AGCCCTCGGT
TTCCTCGTCA
TCTCAGTTCT
CTACCTCAGG
CGGGCCTTGG
GCCCGGGAGC
ACAGGGCGGG
TGAAGAGCCA
GCACCAGGCC
CCGTGACCCC
TGCCCAGTTG
CCCCCACCTC
TGCCGCTGCC
CCCTGCTCGT
TCTGAGTCTG
AGGTGCTAGA
AGAAATCAGT
CGGGCTTGTG
AGACCCTTGT
AGAGCGGGTG
GGCCGGAGTG
CCATCAACCC
AGTGGACACG
GCAGACCCAG
TGGACAGTGG
GACTGAGCCA
CAGCCGCCGC
CTGCGCAGGC
CAAGGAGGAG
AGTGGGGGGG
TGGCGGTAGC
AGCAGCAGGT
CCAGGTAGTG
GCAAGCGTCG
TCAAAAGGAG
CATCGGCGGC
ACAGGCGGGC
CTGTAGGGAC
AGTGTGGGTC
GACGACCCCG
CGAGGGGAGG
GCCAAGGCCA
AGGCCAAGGC
TCCCAAAGAA
AAAAGCCGAA
GGGTGCTGGG
GAACCTCGAC
TTGCAGAGTG
AGGAGATCCA
GGGCCGGGAG
AAGGCCCGGC
CCGATGTCGG
TGGGGTTTCC
AAAGTCAAGA
CACCCACAGC
TCCAGCACCC
CCGCCTGCTC
CTGCACCCGC
TGCTCAGCCA
ACACCCCCAT
CAGCTCCTGT
CCCTGGGAAG
AAGACTCGTG
AGGAGGCTCC
GGGGCCTCCA
GGTGTGAGCC
GGGCAGATAT
GCTGAAGCTC
CGGTCACTTA
GTGAGGGGCC
TCCCAAGGAG
CTGAAGATCA
GGCTCATCAA
GGTGGAAAGT
GGGGACAAGG
AGACCTTTAT
CGCCTCTGAG
GTGGAAGAGC
GGCGGCTGCG
CATGGCAGAC
CTCACCATCA
GCCACTGTGC
CGCCGATGTC
ATGCGTGCCA
GCAAGAATGC
CAAGGTGAAA
GGGAAATTCC
GAGAGTCCTA
CCTGTCCCCT
GCCCAGTCTG
TGAAACCCAA
GATCAACACT
GAGGAGAAGC
TGCCCCGGGA
AAAACTCAAC
CCCCCTACCC
CCAGCATCTA
TTTGGAGAGC
AAACGAGATG
CCTTCTCGCC
GGTCCTGCTA
CAGTTCTGTA
CAGACCCCCG
GAACCCCATC
ACCGTCATCA
GGGGCCTGGC
TGGTTCACTT
CGGCTCAACT
TAGGCCTTTT
CTCCACCAAG
ACTCTGGTGG
AGGCGAGCGG
TGAACATACG
GTGGAGGTCC
GTACCCAAGT
ACAGCAGCCC
TCAGACGAGA
ACTGGGACCT
GACAGGTACC
AGACAAATCT
GGCCCTGTGA
GAGCTCCCGT
TCCCACACCA
CCATCGCTAA
ATACGCACAG
TACCAGGCCT
CGTCCTTCCA
GGAGTCACTG
CAGGAGGAGA
GGGAGAGTGA
GGATGAGGAA
TCCGAGGAAC
CAGACAGCAC
TACAGGAACC
TCTCCCAGCA
GTGCACCGGA
CCCCAAGAAC
CATCACATCA
TCAAGTTTGG
CACTAACATC
GACCTGTCTG
ATGCCAAGAG
GTGGAAGCCA
CAGCTACAGG
AGCTGCTGAA
ACTGCCCGCC
TTCATGCGGG
TAACATCCAC
AGGCAACATG
CTCAGCCACG
TGGGCCACAC
CATCCTGGGC
ATGAACACCG
TGCAGCTATA
CATGAAGGTC
CCTGGCAGCC
GAACGCCAGG
CCACCAAGAG
AATAACAATT
TCTGCTCAGT
CAACATCAAC
ATTGGCCCTG
GGGACTGCGA
GTGGTTCGCG
GTACATGAGC
ACTATTGGGA
GACCATCAGC
GCCTTCTGCG
ACCGGCATGG
TGTGGACTAC
TTGACTGGTT
CCTGGTGGCC
AATCTTGGAT
GACCTCTATG
CGTCCAATAT
TCCTGTTTAC
CGCTTCGTGC
AGCGCCCTGG
AGACCTTGTG
TGGATTAATG
CAGGGACTGT
ACATTGGGTG
CAGGCTACCG
GCTGGTGCAA
CAACATTGCC
TGGAACGTGG
GGCCCCTCAC
CGCCTATCAG
TACCAGCTGG
CCCTGGAGCG
ATATGAGTGG
AACGAGGTGA
AGAACGTCAA
GTCCATTGTG
CCCATGATTC
ATGTGTCCTG
GAACGTCGCT
CGAACGGTCA
AGATCAGCGA
TCCTGACTTG
TTCAAGATGA
TCAAGTTCTG
CCTCCTGCAG
TCAATGAAGC
ACTGTCAGGT
ACAGCGGGAG
AGCCTGGTGC
GGGCAGGGAA
GAAGATCGCT
TACCAAGGCC
GTGTCAAAGA
CGAGCCTGCC
TACTACTGCA
ACGAATGCGA
CGTGGAGGTG
TTCAACATCC
TGTTCGTTAC
AAGTGAGAAT
GGCAGCCGAA
ACACGTACCT
GGTGCACTGC
GAGGGCTGTG
CGCGCCGTCG
CAGCGCGGGC
CTACAGGGCG
TGGTGGTGCT
AGAGCAGTAC
CGCACGGAGG
AGCTGGCGCA
GGCCTACGAT
GCCTTCACAC
TGGCTCCCGC
CAGCACGTCT
CGATGAGGAT
CCGCGGCCGC
GAAGGATCTG
CGATCGCTCC
GGTGCCCGTC
AGTGGGCAGA
GCGCACATCG
CCCACAGTCC
CCGAGAAGTT
GGGGGGAGGG
GTCGGCAATT
GAACGGGTGC
CTAGAGAAGG
TGGCGCGGGG
TAAACTGGGA
AAGTGATGTC
GTGTACTGGC
TCCGCCTTTT
TCCCGAGGGT
GGGGGAGAAC
CGTATATAAG
TGCAGTAGTC
GCCGTGAACG
TTCTTTTTCG
CAACGGGTTT
GCCGCCAGAA
CACAGCTGAA
GCTTCGAGGG
GCTCGCATCT
CTCCTTCACG
CGCCCGCCGC
CCTACCTGAG
GCCGCCATCC
ACGCCGGTTG
AGTCGCGTTC
TGCCGCCTCC
CGCCTGTGGT
GCCTCCTGAA
CTGCGTCCGC
CGTCTAGGTA
AGTTTAAAGC
TCAGGTCGAG
ACCGGGCCTT
TGTCCGGCGC
TCCCTTGGAG
CCTACCTAGA
CTCAGCCGGC
TCTCCACGCT
TTGCCTGACC
CTGCTTGCTC
AACTCTACGT
CTTTGTTTCG
TTTTCTGTTC
TGCGCCGTTA
CAGATCCAAG
CTGTGACCGG
CGCCTACGCT
AGACGCCACC
ATGGAGAGCG
ACGAGAGCGG
CCTGCCCGCC
ATGGAGATCG
AGTGCCGCAT
CACCGGCACC
CTGAACGGCG
TGGAGTTCGA
GCTGGTGGGC
GGCGGAGAGG
GCACCCCCAA
GCAGGGCCGC
ATGACCAACA
AGATGAAGAG
CACCAAAGGC
GCCCTGACCT
TCAGCCCCTA
CCTGCTGAGC
CACGTGATGG
GCTACGGCTT
CTACCACTTC
GGCACCTACC
CCAGCGGCTA
CGAGAACCCC
TTCCTGCACG
CCATCAACAA
CGGCGGCTAC
ACCAACACCC
GCATCGAGAA
GTACGAGGAC
GGCGGCGTGC
TGCACGTGAG
CTTCAGCTAC
CGCTACGAGG
CCGGCCGCGT
GATCGGCGAC
TTCAAGGTGG
TGGGCACCGG
CTTCCCCGAG
GACAGCGTGA
TCTTCACCGA
CAAGATCATC
CGCAGCAACG
CCACCGTGGA
GCACCTGCAC
CCCATGGGCG
ATAACGTGCT
GGTGGGCAGC
TTCGCCCGCA
CCTTCAGCCT
GCGCGACGGC
GGCTACTACA
GCTTCGTGGT
GGACAGCCAC
ATGCACTTCA
AGAGCGCCAT
CCACCCCAGC
ATCCTGCAGA
ACGGGGGCCC
CATGTTCGCC
TTCCGCCGCG
TGGAGGAGCT
GCACAGCAAC
ACCGAGCTGG
GCATCGTGGA
GTACCAGCAC
GCCTTCAAGA
CCCCCATCGC
CTTCGCCAGA
TCCCGCGCTC
AGTCGTCCAA
TTCTGCCGTG
GACGGCACCG
CCGGACCCGG
CTCCACCGGA
TCTCGCTAAG
TCGACAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
ATCATCGTCC
TTTCCTTGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCCTGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAAATAAG
ATCTGCTTTT
TGCTTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTAGTAGTT
CATGTCATCT
TATTATTCAG
TATTTATAAC
TTGCAAAGAA
ATGAATATCA
GAGAGTGAGA
GGAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGGCTCTAG
CTATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CGGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GACTTTTGCA
GAGACGGCCC
AAATTCGTAA
TCATGGTCAT
AGCTGTTTCC
TGTGTGAAAT
TGTTATCCGC
TCACAATTCC
ACACAACATA
CGAGCCGGAA
GCATAAAGTG
TAAAGCCTGG
GGTGCCTAAT
GAGTGAGCTA
ACTCACATTA
ATTGCGTTGC
GCTCACTGCC
CGCTTTCCAG
TCGGGAAACC
TGTCGTGCCA
GCTGCATTAA
TGAATCGGCC
AACGCGCGGG
GAGAGGCGGT
TTGCGTATTG
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGGA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
CAATGCTTAA
TCAGTGAGGC
ACCTATCTCA
GCGATCTGTC
TATTTCGTTC
ATCCATAGTT
GCCTGACTCC
CCGTCGTGTA
GATAACTACG
ATACGGGAGG
GCTTACCATC
TGGCCCCAGT
GCTGCAATGA
TACCGCGAGA
CCCACGCTCA
CCGGCTCCAG
ATTTATCAGC
AATAAACCAG
CCAGCCGGAA
GGGCCGAGCG
CAGAAGTGGT
CCTGCAACTT
TATCCGCCTC
CATCCAGTCT
ATTAATTGTT
GCCGGGAAGC
TAGAGTAAGT
AGTTCGCCAG
TTAATAGTTT
GCGCAACGTT
GTTGCCATTG
CTACAGGCAT
CGTGGTGTCA
CGCTCGTCGT
TTGGTATGGC
TTCATTCAGC
TCCGGTTCCC
AACGATCAAG
GCGAGTTACA
TGATCCCCCA
TGTTGTGCAA
AAAAGCGGTT
AGCTCCTTCG
GTCCTCCGAT
CGTTGTCAGA
AGTAAGTTGG
CCGCAGTGTT
ATCACTCATG
GTTATGGCAG
CACTGCATAA
TTCTCTTACT
GTCATGCCAT
CCGTAAGATG
CTTTTCTGTG
ACTGGTGAGT
ACTCAACCAA
GTCATTCTGA
GAATAGTGTA
TGCGGCGACC
GAGTTGCTCT
TGCCCGGCGT
CAATACGGGA
TAATACCGCG
CCACATAGCA
GAACTTTAAA
AGTGCTCATC
ATTGGAAAAC
GTTCTTCGGG
GCGAAAACTC
TCAAGGATCT
TACCGCTGTT
GAGATCCAGT
TCGATGTAAC
CCACTCGTGC
ACCCAACTGA
TCTTCAGCAT
CTTTTACTTT
CACCAGCGTT
TCTGGGTGAG
CAAAAACAGG
AAGGCAAAAT
GCCGCAAAAA
AGGGAATAAG
GGCGACACGG
AAATGTTGAA
TACTCATACT
CTTCCTTTTT
CAATATTATT
GAAGCATTTA
TCAGGGTTAT
TGTCTCATGA
GCGGATACAT
ATTTGAATGT
ATTTAGAAAA
ATAAACAAAT
AGGGGTTCCG
CGCACATTTC
CCCGAAAAGT
GCCACCTGAC
GTCTAAGAAA
CCATTATTAT
CATGACATTA
ACCTATAAAA
ATAGGCGTAT
CACGAGGCCC
TTTCGTCTCG
CGCGTTTCGG
TGATGACGGT
GAAAACCTCT
GACACATGCA
GCTCCCGGAG
ACGGTCACAG
CTTGTCTGTA
AGCGGATGCC
GGGAGCAGAC
AAGCCCGTCA
GGGCGCGTCA
GCGGGTGTTG
GCGGGTGTCG
GGGCTGGCTT
AACTATGCGG
CATCAGAGCA
GATTGTACTG
AGAGTGCACC
ATATGCGGTG
TGAAATACCG
CACAGATGCG
TAAGGAGAAA
ATACCGCATC
AGGCGCCATT
CGCCATTCAG
GCTGCGCAAC
TGTTGGGAAG
GGCGATCGGT
GCGGGCCTCT
TCGCTATTAC
GCCAGCTGGC
GAAAGGGGGA
TGTGCTGCAA
GGCGATTAAG
TTGGGTAACG
CCAGGGTTTT
CCCAGTCACG
ACGTTGTAAA
ACGACGGCCA
GTGCCAAGCT
G