名称: | pYES2-UBR2(human)-3×FLAG |
---|---|
别称: | NM_015255.3;bA49A4.1; C6orf133; dJ242G1.1; dJ392M17.3 |
基因长: | 5265bp |
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | URA3 |
荧光标记: | |
启动子: | GAL1 |
复制子: | pUC,2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pYES2 |
正向引物: | GAL1-Profor |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pYES2-UBR2(human)-3×FLAG是适用于酵母菌过表达的质粒。质粒携带GAL1启动子可启动人源UBR2基因的表达,促进UBR2基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化。质粒携带URA3筛选标记,用于筛选出表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGGATTAGA
AGCCGCCGAG
CGGGTGACAG
CCCTCCGAAG
GAAGACTCTC
CTCCGTGCGT
CCTCGTCTTC
ACCGGTCGCG
TTCCTGAAAC
GCAGATGTGC
CTCGCGCCGC
ACTGCTCCGA
ACAATAAAGA
TTCTACAATA
CTAGCTTTTA
TGGTTATGAA
GAGGAAAAAT
TGGCAGTAAC
CTGGCCCCAC
AAACCTTCAA
ATGAACGAAT
CAAATTAACA
ACCATAGGAT
GATAATGCGA
TTAGTTTTTT
AGCCTTATTT
CTGGGGTAAT
TAATCAGCGA
AGCGATGATT
TTTGATCTAT
TAACAGATAT
ATAAATGCAA
AAACTGCATA
ACCACTTTAA
CTAATACTTT
CAACATTTTC
GGTTTGTATT
ACTTCTTATT
CAAATGTAAT
AAAAGTATCA
ACAAAAAATT
GTTAATATAC
CTCTATACTT
TAACGTCAAG
GAGAAAAAAC
CCCGGATCGG
ACTACTAGCA
GCTGTAATAC
GACTCACTAT
AGGGAATATT
AAGCTTGGTA
CCGAGCTCGG
ATCCGCCACC
ATGGCGTCGG
AGCTAGAGCC
AGAGGTGCAG
GCCATCGACC
GGAGCTTGCT
GGAATGTTCG
GCCGAGGAGA
TTGCGGGGAA
ATGGCTGCAA
GCAACTGACC
TCACTAGAGA
AGTGTACCAG
CATTTAGCCC
ACTATGTACC
CAAAATCTAC
TGCAGGGGTC
CCAACCCTTT
TCCACAGAAA
GAAGACATGC
TGGCACAGCA
TGTTTTGTTG
GGACCAATGG
AATGGTACCT
TTGTGGTGAA
GATCCTGCAT
TTGGATTTCC
AAAACTTGAG
CAAGCAAACA
AACCTTCTCA
TCTTTGTGGT
CGTGTTTTTA
AAGTAGGAGA
GCCTACATAT
TCTTGCAGAG
ACTGTGCAGT
TGATCCAACT
TGTGTTTTGT
GCATGGAGTG
CTTTTTGGGA
AGTATTCACA
GAGATCATCG
ATATAGGATG
ACAACATCAG
GAGGTGGAGG
TTTCTGTGAC
TGTGGTGATA
CTGAAGCCTG
GAAAGAGGGT
CCTTACTGTC
AAAAACATGA
ACTTAACACC
TCTGAAATTG
AGGAAGAAGA
GGATCCTCTT
GTTCATTTAT
CAGAAGATGT
GATAGCAAGA
ACTTATAACA
TTTTTGCTAT
TACGTTTCGG
TATGCAGTAG
AAATATTAAC
CTGGGAAAAA
GAAAGTGAAT
TGCCAGCAGA
TTTAGAGATG
GTAGAGAAGA
GTGACACCTA
CTATTGCATG
CTGTTTAATG
ATGAGGTTCA
CACCTATGAA
CAAGTTATTT
ATACTCTTCA
GAAAGCTGTT
AACTGTACAC
AAAAAGAAGC
TATTGGTTTT
GCAACTACAG
TAGATCGAGA
TGGGCGTAGG
TCTGTTCGAT
ATGGAGATTT
TCAGTATTGT
GAGCAAGCAA
AATCAGTAAT
TGTGAGAAAT
ACCAGTAGAC
AGACAAAGCC
ACTCAAAGTT
CAAGTTATGC
ATTCGTCTAT
TGTCGCACAT
CAGAATTTTG
GTTTGAAACT
TTTGTCTTGG
CTGGGAAGTA
TTATTGGATA
TTCAGATGGC
CTTCGCCGGA
TTTTATGTCA
AGTTGGTTTA
CAAGAAGGGC
CAGATGGTGA
AAACTCTTCT
CTAGTGGACA
GACTGATGCT
TAGTGATTCC
AAATTATGGA
AAGGTGCTAG
GAGTGTATAT
CATCAGTTGT
TCATGAGCAG
TCTGCTTATG
GATTTGAAAT
ACAAGAAACT
ATTTGCTGTT
CGATTTGCAA
AAAATTACCA
GCAGTTGCAG
AGAGATTTTA
TGGAGGATGA
TCACGAGCGA
GCAGTGTCGG
TGACTGCTCT
ATCTGTCCAG
TTCTTCACCG
CACCTACTCT
GGCTCGAATG
CTCATCACAG
AAGAAAACTT
AATGAGCATT
ATCATTAAGA
CTTTTATGGA
TCATTTGAGA
CATCGAGATG
CCCAGGGCAG
ATTTCAGTTT
GAACGATACA
CTGCTTTACA
AGCCTTCAAA
TTTAGGAGAG
TACAGAGCCT
TATTTTAGAT
CTCAAGTATG
TGTTAATTAG
CAAACCAACT
GAATGGTCAG
ATGAGCTGAG
GCAGAAGTTC
CTAGAAGGGT
TTGATGCCTT
TTTGGAATTA
CTAAAATGTA
TGCAGGGAAT
GGATCCAATT
ACACGTCAAG
TAGGACAACA
TATTGAAATG
GAACCAGAGT
GGGAAGCAGC
CTTCACACTA
CAAATGAAAT
TAACACATGT
CATTTCAATG
ATGCAGGACT
GGTGTGCTTC
AGATGAAAAA
GTGTTAATCG
AAGCTTACAA
GAAATGTCTC
GCTGTACTGA
TGCAGTGTCA
TGGTGGTTAT
ACTGATGGTG
AACAGCCAAT
CACACTAAGC
ATTTGTGGAC
ATTCAGTGGA
AACTATCAGA
TACTGTGTTT
CCCAAGAAAA
AGTTAGCATT
CACCTCCCAG
TTTCTCGCTT
ACTTGCAGGT
TTACATGTAT
TATTAAGCAA
AAGTGAAGTG
GCATATAAAT
TTCCAGAGCT
CCTACCTCTA
AGTGAACTTA
GCCCACCCAT
GTTGATAGAA
CACCCTCTTA
GATGTCTTGT
TCTGTGTGCC
CAAGTACATG
CCGGAATGTG
GAGAAGAAAT
GGGTTCTCTC
TAGTAAACCA
GATTTATTAC
TACCATAATG
TGAAATGCAG
ACGTGAGATG
TTTGACAAGG
ATGTAGTAAT
GCTTCAGACA
GGTGTCTCCA
TGATGGATCC
AAATCATTTC
CTGATGATCA
TGCTCAGCCG
CTTTGAACTT
TATCAGATTT
TCAGTACTCC
AGACTATGGA
AAAAGATTTA
GTTCTGAGAT
TACCCATAAG
GATGTTGTTC
AGCAGAACAA
TACTCTAATA
GAAGAAATGC
TATACCTCAT
TATAATGCTT
GTTGGAGAGA
GATTTAGTCC
TGGAGTTGGA
CAGGTAAATG
CTACAGATGA
AATCAAGCGA
GAGATTATCC
ATCAGTTGAG
TATCAAGCCT
ATGGCTCATA
GTGAATTGGT
AAAGTCTTTA
CCTGAAGATG
AGAACAAGGA
GACTGGCATG
GAGAGTGTAA
TCGAAGCAGT
TGCCCATTTC
AAGAAACCTG
GATTAACAGG
ACGAGGCATG
TATGAACTGA
AACCAGAATG
TGCCAAAGAG
TTCAACTTGT
ATTTCTATCA
CTTTTCAAGG
GCAGAACAGT
CCAAGGCAGA
AGAAGCGCAA
CGGAAATTGA
AAAGACAAAA
TAGAGAAGAT
ACAGCACTCC
CACCTCCGGT
GTTGCCTCCA
TTCTGCCCTC
TGTTTGCAAG
CCTGGTTAAC
ATTTTGCAGT
CAGATGTCAT
GTTGTGCATC
ATGGGAACAA
TTCTGCAATG
GGCTGTGGAA
CATAATGGAT
ATGCCTGGTC
AGAGTCCATG
CTGCAAAGGG
TGTTACATTT
AATTGGCATG
GCACTACAAG
AAGAAAAACA
ACATTTAGAG
AATGTCACGG
AAGAGCATGT
AGTAACATTT
ACCTTCACTC
AGAAGATATC
AAAACCTGGT
GAAGCGCCAA
AAAATTCTCC
TAGCATACTA
GCTATGCTGG
AAACACTACA
AAATGCTCCC
TACCTAGAAG
TCCACAAAGA
CATGATTCGG
TGGATATTGA
AGACTTTTAA
TGCTGTTAAA
AAGATGAGGG
AGAGTTCACC
TACCAGTCCC
GTGGCAGAGA
CAGAAGGAAC
CATAATGGAA
GAGAGTTCAA
GGGACAAAGA
CAAAGCTGAG
AGGAAGAGAA
AAGCAGAGAT
TGCCAGACTG
CGCAGAGAAA
AGATCATGGC
TCAGATGTCT
GAAATGCAGC
GGCATTTTAT
TGATGAAAAC
AAAGAACTCT
TTCAGCAGAC
ATTAGAACTG
GATGCCTCAA
CCTCTGCTGT
TCTTGATCAT
AGCCCTGTGG
CTTCAGATAT
GACACTTACA
GCACTGGGCC
CCGCACAAAC
TCAGGTTCCT
GAACAAAGAC
AATTCGTTAC
ATGTATATTG
TGTCAAGAGG
AGCAAGAAGT
TAAAGTGGAA
AGCAGGGCAA
TGGTCTTGGC
AGCATTTGTT
CAGAGATCAA
CTGTATTATC
AAAAAACAGA
AGTAAATTTA
TTCAAGATCC
AGAAAAATAT
GATCCATTAT
TCATGCACCC
TGATCTGTCT
TGTGGAACAC
ACACTAGTAG
CTGTGGGCAC
ATTATGCATG
CCCATTGTTG
GCAAAGGTAT
TTTGATTCCG
TTCAAGCTAA
AGAACAGCGA
AGGCAACAGA
GATTACGCTT
ACATACGAGC
TATGATGTAG
AAAACGGAGA
ATTCCTTTGC
CCCCTTTGTG
AATGCTTGAG
TAATACTGTT
ATTCCTCTGC
TGCTTCCTCC
AAGAAATATT
TTTAACAACA
GGTTAAATTT
TTCAGACCAA
CCAAATCTGA
CTCAGTGGAT
TAGAACAATA
TCTCAGCAAA
TAAAAGCATT
ACAGTTTCTT
AGGAAAGAAG
AAAGTACTCC
TAATAATGCC
TCTACAAAGA
ATTCAGAAAA
TGTGGATGAA
TTACAGCTCC
CTGAAGGGTT
CAGGCCTGAT
TTTCGTCCTA
AGATCCCTTA
TTCTGAGAGC
ATAAAAGAAA
TGCTAACGAC
ATTTGGAACT
GCTACCTACA
AGGTGGGACT
AAAGGTTCAT
CCCAATGAAG
AGGATCCTCG
TGTTCCCATA
ATGTGTTGGG
GTAGCTGCGC
GTACACCATC
CAAAGCATAG
AAAGAATTTT
GAGTGATGAA
GATAAACCAT
TGTTTGGTCC
TTTACCTTGC
AGACTGGATG
ACTGTCTTAG
GTCATTGACG
AGATTTGCCG
CAGCACACTG
GACAGTGGCA
TCAGTTTCAG
TGGTGCAAGG
ACATTTTTGT
AAACTTTTTG
CATCACTGGT
GCCTAATGAC
AGCCATGAGG
AACTTCCATG
CATATTAGAT
ATTGACATGT
TTCATTTATT
GGTGGGCTTG
GTGCTTGCAT
TTCCTGCGTT
GCAGTGTCAG
GATTTTTCAG
GGATCAGCCT
TGGCACTGGA
GACCTTCACA
TTTTCCATCT
GGTTACTATG
GCACACATCA
TACAGATCTT
ACTTACCTCA
TGTACAGAAG
AGAATGGCAT
GGATCAAGAA
AATCCCCCTT
GTGAAGAAGA
ATCAGCAGTT
CTTGCTTTGT
ATAAAACACT
TCACCAGTAT
ACGGGAAGTG
CCTTGAAAGA
AATACCATCC
GGCTGGCATC
TGTGGAGGAG
TGTCAGAGCT
GGAATCATGC
CTTTCCTGAA
GTGTTCTGCT
TTATTTTTTC
ATTACTTAAA
TGGAGTTCCT
TCCCCACCCG
ACATTCAAGT
TCCTGGAACA
AGCCATTTTG
AACATTTATG
TAGCTATCTT
TCCCTACCAA
ACAACCTCAT
TTGCCTTTTT
CAAGAAAATA
GTGAGATAAT
GAATTCACTG
ATTGAAAGTT
GGTGCCGTAA
CAGTGAAGTT
AAAAGATATC
TAGAAGGTGA
AAGAGATGCT
ATAAGATATC
CAAGAGAATC
TAACAAATTA
ATAAACCTTC
CAGAGGATTA
CAGCAGCCTC
ATTAATCAAG
CATCCAATTT
CTCGTGCCCG
AAATCAGGTG
GTGATAAGAG
CAGAGCCCCA
ACTCTGTGCC
TTGTGTGCGG
ATCTCTGCTG
TGCTCCCAGA
GTTACTGCTG
CCAGACTGAA
CTGGAAGGGG
AGGATGTAGG
AGCCTGCACA
GCTCACACCT
ACTCCTGTGG
CTCTGGAGTG
GGCATCTTCC
TGAGAGTACG
GGAATGTCAG
GTGCTATTTT
TAGCTGGCAA
AACCAAAGGC
TGTTTTTATT
CTCCTCCTTA
CCTTGATGAC
TATGGGGAGA
CCGACCAGGG
ACTCAGACGG
GGAAATCCTT
TACATTTATG
CAAAGAGCGA
TTCAAGAAGA
TTCAGAAGCT
CTGGCACCAA
CACAGTGTCA
CAGAGGAAAT
TGGACATGCA
CAGGAAGCCA
ATCAGACACT
GGTTGGCATT
GACTGGCAAC
ATTTAGGATC
CGACTACAAA
GACCATGACG
GTGATTATAA
AGATCATGAC
ATCGACTACA
AGGATGACGA
TGACAAGTAG
GAATTCTGCA
GATATCCATC
ACACTGGCGG
CCGCTCGAGC
ATGCATCTAG
AGGGCCGCAT
CATGTAATTA
GTTATGTCAC
GCTTACATTC
ACGCCCTCCC
CCCACATCCG
CTCTAACCGA
AAAGGAAGGA
GTTAGACAAC
CTGAAGTCTA
GGTCCCTATT
TATTTTTTTA
TAGTTATGTT
AGTATTAAGA
ACGTTATTTA
TATTTCAAAT
TTTTCTTTTT
TTTCTGTACA
GACGCGTGTA
CGCATGTAAC
ATTATACTGA
AAACCTTGCT
TGAGAAGGTT
TTGGGACGCT
CGAAGGCTTT
AATTTGCAAG
CTGCGGCCCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGC
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGCGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GGCATTGCTA
CAGGCATCGT
GGTGTCACTC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TAGTGTATCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TGGGTAATAA
CTGATATAAT
TAAATTGAAG
CTCTAATTTG
TGAGTTTAGT
ATACATGCAT
TTACTTATAA
TACAGTTTTT
TAGTTTTGCT
GGCCGCATCT
TCTCAAATAT
GCTTCCCAGC
CTGCTTTTCT
GTAACGTTCA
CCCTCTACCT
TAGCATCCCT
TCCCTTTGCA
AATAGTCCTC
TTCCAACAAT
AATAATGTCA
GATCCTGTAG
AGACCACATC
ATCCACGGTT
CTATACTGTT
GACCCAATGC
GTCTCCCTTG
TCATCTAAAC
CCACACCGGG
TGTCATAATC
AACCAATCGT
AACCTTCATC
TCTTCCACCC
ATGTCTCTTT
GAGCAATAAA
GCCGATAACA
AAATCTTTGT
CGCTCTTCGC
AATGTCAACA
GTACCCTTAG
TATATTCTCC
AGTAGATAGG
GAGCCCTTGC
ATGACAATTC
TGCTAACATC
AAAAGGCCTC
TAGGTTCCTT
TGTTACTTCT
TCTGCCGCCT
GCTTCAAACC
GCTAACAATA
CCTGGGCCCA
CCACACCGTG
TGCATTCGTA
ATGTCTGCCC
ATTCTGCTAT
TCTGTATACA
CCCGCAGAGT
ACTGCAATTT
GACTGTATTA
CCAATGTCAG
CAAATTTTCT
GTCTTCGAAG
AGTAAAAAAT
TGTACTTGGC
GGATAATGCC
TTTAGCGGCT
TAACTGTGCC
CTCCATGGAA
AAATCAGTCA
AGATATCCAC
ATGTGTTTTT
AGTAAACAAA
TTTTGGGACC
TAATGCTTCA
ACTAACTCCA
GTAATTCCTT
GGTGGTACGA
ACATCCAATG
AAGCACACAA
GTTTGTTTGC
TTTTCGTGCA
TGATATTAAA
TAGCTTGGCA
GCAACAGGAC
TAGGATGAGT
AGCAGCACGT
TCCTTATATG
TAGCTTTCGA
CATGATTTAT
CTTCGTTTCC
TGCAGGTTTT
TGTTCTGTGC
AGTTGGGTTA
AGAATACTGG
GCAATTTCAT
GTTTCTTCAA
CACTACATAT
GCGTATATAT
ACCAATCTAA
GTCTGTGCTC
CTTCCTTCGT
TCTTCCTTCT
GTTCGGAGAT
TACCGAATCA
AAAAAATTTC
AAAGAAACCG
AAATCAAAAA
AAAGAATAAA
AAAAAAATGA
TGAATTGAAT
TGAAAAGCTA
GCTTATCGAT
GATAAGCTGT
CAAAGATGAG
AATTAATTCC
ACGGACTATA
GACTATACTA
GATACTCCGT
CTACTGTACG
ATACACTTCC
GCTCAGGTCC
TTGTCCTTTA
ACGAGGCCTT
ACCACTCTTT
TGTTACTCTA
TTGATCCAGC
TCAGCAAAGG
CAGTGTGATC
TAAGATTCTA
TCTTCGCGAT
GTAGTAAAAC
TAGCTAGACC
GAGAAAGAGA
CTAGAAATGC
AAAAGGCACT
TCTACAATGG
CTGCCATCAT
TATTATCCGA
TGTGACGCTG
CAGCTTCTCA
ATGATATTCG
AATACGCTTT
GAGGAGATAC
AGCCTAATAT
CCGACAAACT
GTTTTACAGA
TTTACGATCG
TACTTGTTAC
CCATCATTGA
ATTTTGAACA
TCCGAACCTG
GGAGTTTTCC
CTGAAACAGA
TAGTATATTT
GAACCTGTAT
AATAATATAT
AGTCTAGCGC
TTTACGGAAG
ACAATGTATG
TATTTCGGTT
CCTGGAGAAA
CTATTGCATC
TATTGCATAG
GTAATCTTGC
ACGTCGCATC
CCCGGTTCAT
TTTCTGCGTT
TCCATCTTGC
ACTTCAATAG
CATATCTTTG
TTAACGAAGC
ATCTGTGCTT
CATTTTGTAG
AACAAAAATG
CAACGCGAGA
GCGCTAATTT
TTCAAACAAA
GAATCTGAGC
TGCATTTTTA
CAGAACAGAA
ATGCAACGCG
AAAGCGCTAT
TTTACCAACG
AAGAATCTGT
GCTTCATTTT
TGTAAAACAA
AAATGCAACG
CGACGAGAGC
GCTAATTTTT
CAAACAAAGA
ATCTGAGCTG
CATTTTTACA
GAACAGAAAT
GCAACGCGAG
AGCGCTATTT
TACCAACAAA
GAATCTATAC
TTCTTTTTTG
TTCTACAAAA
ATGCATCCCG
AGAGCGCTAT
TTTTCTAACA
AAGCATCTTA
GATTACTTTT
TTTCTCCTTT
GTGCGCTCTA
TAATGCAGTC
TCTTGATAAC
TTTTTGCACT
GTAGGTCCGT
TAAGGTTAGA
AGAAGGCTAC
TTTGGTGTCT
ATTTTCTCTT
CCATAAAAAA
AGCCTGACTC
CACTTCCCGC
GTTTACTGAT
TACTAGCGAA
GCTGCGGGTG
CATTTTTTCA
AGATAAAGGC
ATCCCCGATT
ATATTCTATA
CCGATGTGGA
TTGCGCATAC
TTTGTGAACA
GAAAGTGATA
GCGTTGATGA
TTCTTCATTG
GTCAGAAAAT
TATGAACGGT
TTCTTCTATT
TTGTCTCTAT
ATACTACGTA
TAGGAAATGT
TTACATTTTC
GTATTGTTTT
CGATTCACTC
TATGAATAGT
TCTTACTACA
ATTTTTTTGT
CTAAAGAGTA
ATACTAGAGA
TAAACATAAA
AAATGTAGAG
GTCGAGTTTA
GATGCAAGTT
CAAGGAGCGA
AAGGTGGATG
GGTAGGTTAT
ATAGGGATAT
AGCACAGAGA
TATATAGCAA
AGAGATACTT
TTGAGCAATG
TTTGTGGAAG
CGGTATTCGC
AATGGGAAGC
TCCACCCCGG
TTGATAATCA
GAAAAGCCCC
AAAAACAGGA
AGATTGTATA
AGCAAATATT
TAAATTGTAA
ACGTTAATAT
TTTGTTAAAA
TTCGCGTTAA
ATTTTTGTTA
AATCAGCTCA
TTTTTTAACG
AATAGCCCGA
AATCGGCAAA
ATCCCTTATA
AATCAAAAGA
ATAGACCGAG
ATAGGGTTGA
GTGTTGTTCC
AGTTTCCAAC
AAGAGTCCAC
TATTAAAGAA
CGTGGACTCC
AACGTCAAAG
GGCGAAAAAG
GGTCTATCAG
GGCGATGGCC
CACTACGTGA
ACCATCACCC
TAATCAAGTT
TTTTGGGGTC
GAGGTGCCGT
AAAGCAGTAA
ATCGGAAGGG
TAAACGGATG
CCCCCATTTA
GAGCTTGACG
GGGAAAGCCG
GCGAACGTGG
CGAGAAAGGA
AGGGAAGAAA
GCGAAAGGAG
CGGGGGCTAG
GGCGGTGGGA
AGTGTAGGGG
TCACGCTGGG
CGTAACCACC
ACACCCGCCG
CGCTTAATGG
GGCGCTACAG
GGCGCGTGGG
GATGATCCAC
TAGT