名称: | pTol2-ubiquitin-EGFP-nr5a1b(zebrafish) |
---|---|
别称: | - |
基因长: | 1509bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 转座,蛋白表达 |
片段类型: | ORF |
片段物种: | 斑马鱼 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | |
复制子: | |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | zubiquitin-GFP-2A-pTol2 |
正向引物: | pEGFP-C-5' |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pTol2-ubiquitin-EGFP-nr5a1b(zebrafish)是适用于哺乳细胞过表达的质粒。质粒携带Tol2 转座子系统,可高效整合斑马鱼nr5a1b基因到宿主基因组。质粒携带的Ubiquitin基因便于研究蛋白质降解,调节细胞周期等。质粒携带的EGFP绿色荧光蛋白,便于直观地观察质粒转染效果。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCACCTAAAT
TGTAAGCGTT
AATATTTTGT
TAAAATTCGC
GTTAAATTTT
TGTTAAATCA
GCTCATTTTT
TAACCAATAG
GCCGAAATCG
GCAAAATCCC
TTATAAATCA
AAAGAATAGA
CCGAGATAGG
GTTGAGTGTT
GTTCCAGTTT
GGAACAAGAG
TCCACTATTA
AAGAACGTGG
ACTCCAACGT
CAAAGGGCGA
AAAACCGTCT
ATCAGGGCGA
TGGCCCACGC
TGGCTAAGAA
CTCATCAGCC
TCCCCGGTCC
ATCTACCCAC
GTACCAATGC
ACCAATTGGC
CACAATGACG
GCTACTACAT
GGTGCCATTC
CTTCCTCTTT
ATAGGAATGG
AGACTACCTC
CTGTCCAACA
AGGCTCTTGG
ATACGAGTAC
GCCTACCTGT
TGGACCCAGG
TCATTGCACA
ACACCAGAAA
TGCCCTCTGA
TCTGCAAAAG
ACGTGAATAT
CTGTTCAGAC
ACCCATATCC
ACTCTGTTCC
ACACAGGTCA
GAGGTTTGTC
CAGGAGTTCT
TGACAGAGGT
GTAAAAAGTA
CTCAAAAATT
TTACTCAAGT
GAAAGTACAA
GTACTTAGGG
AAAATTTTAC
TCAATTAAAA
GTAAAAGTAT
CTGGCTAGAA
TCTTACTTGA
GTAAAAGTAA
AAAAGTACTC
CATTAAAATT
GTACTTGAGT
ATTAAGGAAG
TAAAAGTAAA
AGCAAGAAAG
AAAACTAGAG
ATTCTTGTTT
AAGCTTTTAA
TCTCAAAAAA
CATTAAATGA
AATGCATACA
AGGTTTTATC
CTGCTTTAGA
ACTGTTTGTA
TTTAATTATC
AAACTATAAG
ACAGACAATC
TAATGCCAGT
ACACGCTACT
CAAAGTTGTA
AAACCTCAGA
TTTAACTTCA
GTAGAAGCTG
ATTCTCAAAA
TTGTTAGTGT
CAAGCCTAGC
TCTTTTGGGG
CTGAAAAGCA
ATCCTGCAGT
GCTGAAAAGC
CTCTCACAGG
CAGCCGATGC
GGGAAGAGGT
GTATTAGTCT
TGATAGAGAG
GCTGCAAATA
GCAGGAAACG
TGAGCAGAGA
CTCCCTGGTG
TCTGAAACAC
AGGCCAGATG
GGCCCTCGAG
ACCAGCAAAG
TTCTAGAATT
TGTCGAAACA
TTTATGTTAT
ATATTTCCTG
AAAAAAATTC
TGAGTAAGTT
CTTAAGTGTT
ATTGCCAGCA
ACATAAACAA
CAGACGGCAA
AATGAATAAA
TGATAACAAA
GCAGTAGGCT
TAAATAAACC
TAATTTTTAT
AGGCTGTTCT
CTACAACCCT
CAAACAGTGA
TTAGTTTTGT
ACTTATAAAC
TTGCCCTTTC
ATTCATATTT
CAAGAAAATT
GGTTCAGAAG
ATCTGGATAT
TCTAGCAGTT
GTTCAAGCTC
ATGGAGGGAT
CAGTGACCTG
ATTCCACAAT
GACTAGGCCT
AATCCAGAAA
TTAGATGACT
GTCAACATAA
AAAGGCACAG
CACTCACTAG
CTGCCCTATA
TATTTTATTA
TATTTTACAT
ATATTATTTT
ATTTATTTAG
CTCTGAGTGC
TGTACTTTCT
GGTTAAAGAA
AACTGCTTAC
AACAGCTAAC
CTGTACTACC
TCAGGCTCAG
GGAATTTGGA
ACAGGTTTGT
CTGGTTTGTT
TCTTTAACCA
TGCATGCTTG
TTTTCAACTA
TGGCAACACA
GTCACATGGG
ACATTACAGA
AATGATTTGT
CGATGACATG
CGACTTTTCT
TTAATAAAGC
GCAAAGATCC
CAAAAAGCAA
ACTTTTAACA
AAAATCATAT
AATTATATTT
TCAATCCAGC
TTTGTAGCAA
CTTTGTGCTG
CTGTTCACTC
AGCAACAGAT
AGTCAGTATA
AGGTCAGTGT
GTCTCAAAGC
AGTGCCATCT
GTTTCACACA
TTGCGTTCTA
TATATAAGTG
TGCTGGTTGA
CACGACACTG
TATAAGGCCT
AGGCTAAAAC
ACAAACAATG
TAGAATGACA
CTGTGTTTTT
TTTGTAAACA
AATGTTGTTT
TTGGTTAAAC
ATCTTTGTGA
AAACATCCTC
CTGTCATGTA
TTTGCTATAT
TCAAATGTTA
AACCCGTGCA
GAATAGAACA
TATACAAAAA
AAAACAACAC
AACACATTTT
TAAACATTAT
TAAATATCAA
GTATTGCTGG
CAGTTCTGTT
TCTGTTTTAC
AGTACCCTTT
GCCACAGTTC
TCCGCTTTTC
CTGGTCCAGA
TTCCACAAGT
CTGATTCACC
AATAGCAAAG
CGAATAAACA
ACCAAAGCAG
CCAATCACTG
CTTGTAGACT
GTCCTGCGAG
ACCGGCCCAT
TCCAGCACAT
TCTGGAAACT
TCCTTTATAT
GATAATTATA
AATACATTTA
AATTATTGAT
ACAAAACATG
TAATTCCTAG
AACATAACCA
TAGCAATCAT
TAGTTTTCAG
GGTAATTATG
TATTTTTAGG
ATTTGACTGC
GGAAAGATCT
GGTCATGTGA
CGTCTCATGA
ACGTCACGGC
CCTGGGTTTC
TATAAATACA
GTAGGACTCT
CGACCATCGG
CAGATTTTTC
GAAGAAGAAG
ATCAGTTTCA
GGAGCCGTAC
TGTTCCGTTT
CAACGCAAAT
ATTAACGGTA
AGAGCGAATT
TCCTAGTTTG
TTTTCATGCC
ATTCTTTAAA
ACCATAGCGT
ATTACTTTAA
TTATAGTAAA
CTTTCGCTTT
CTTTATTACA
AGAGACGTTT
TGTGTTGATT
CTCCGCGGAC
ATTTTCGGTC
AGACAATCAG
AAAATGACCG
CGGAGGACCA
GTAACTTGCA
TTACACGTAA
GTTAAATCTT
CGTGTATTAA
AATGGTTAGG
TTGTTAACGT
CAAATAGGTT
ACCGTGTTTG
CGTGTGATCA
GGTTGGTTTT
GTTAGATTTT
TGTCAGTATT
TTTAATTTAT
TTGTTTTAGT
TTATTTATTT
TTTTTGCTGA
ATCATAGTTT
GTGAACAAAG
AACCCGGATG
TTACATACAG
TACAGCCGCC
ATGTTACAGA
GAGTTATAAC
TTAATCATTT
TAAAAATAAT
TTTGCCTTAC
TTTTAGTTTG
TCATGTTGAG
AAATGAGGAA
ATGTTAAAAT
GAGGAAATAT
CCAATTAATT
TAATATATCA
AAATAATCCA
TGATTACAAT
GCACTGAACT
GGAGAAAATT
AAGATGTTTT
CTAGTGTCAT
GAAACAAATG
TAAGAGATGT
ACATTGTAGA
TGTTTTATGT
CAAGAATTGG
CTAGTTGATG
CAGCATACTG
GCGATACTCA
GTTGTAATAA
CAGTAACGTT
ACATGTTAAT
AGACTACTGA
GTATGCTGTT
CTGTCTATGT
ATGCTCTGTA
AGCTACGAGA
AGGACTTTTT
TAAACAGTAA
AGGGTGCAAT
ATTTTTACAA
ATTGAATTAA
ATAAAGGCTG
TCTATTAAGT
AATATGCTTG
ATATTTTTCT
TACTTGATCG
GAAATAAGAA
AAAATATAAA
CGTTGTTGCT
CTAAAAATCC
TAGTTCAGTT
TAGCCAACCA
CAAATACCTT
TTTGTTCCTC
CAACAGTTTT
TTTTTCTTCT
CTATAATATT
TGGCAGTCTA
TAGTACTCCA
AATGTTTCCC
CACAGTCTAA
CTAATTGGTA
CAGCCAAAAT
CATGACACTT
ATTGCAATAA
TAATTTTGGT
TCATTGGCAT
TGTTGATAGC
CTGTGCCACT
AATATGGTCG
ATTGATCATG
CTTCAGGAAG
AAAACTATAT
TGTTTGATGT
AAGATTATTA
AATCTTCACC
TGCTTCCATT
ACAAACTATT
CCCATCTTAT
TGAATTCTGG
TATGTCTTAA
AGGATTAGTT
CACTTCCCAA
ATCAAAATTT
ACTTAGCCTT
TTTTCATCCA
TGATCCCTTT
TTTTCATCAT
TAATGAAGAA
ATTGTTTTTG
AAAAAGTTTC
AAGATTTTTT
TCTCTATATT
GTGGAGCTTG
TTAGTTTAAA
ATTCCAAAAT
GCAATATGTG
GCTTCAAATG
GTTCTAAATG
ATCCCAGTCA
AGGAATAACA
GTCTTATCTA
ATGAAACCAT
TAGACCTTTA
AAAATAAAAA
AAATAAAAGT
ATTTATTTTT
AAATGACTGA
GTGATTAAGT
TGAATTTCAG
CGTTTCCTTA
CTGTGTAGAA
GTCCTTCCTT
ACTGGCCCCA
CCCTTTGGTT
CTCTGCCAAT
CTGCTACCTA
ATGTAATGTT
GTGGAACATT
ATTATTCTTT
ATTTCTTAAT
TTTTTATTTT
TTATTTTAAA
ACAATGTAAA
CTGCACAGAT
GTGCAGTTTG
TTTAAAATGG
CCAATGCTTT
GGAAATGCAT
GACATAATTA
GATTTCATGA
TGCACAAAGC
CAAATCTCAG
AGCTTGTGCA
AAATGAGCTA
TCATTTCACT
AGGTAAGACC
CTAAATTTTC
ATATAGGATC
ATTTGGACAA
TTTTGCTGCA
GGTAAAATGC
ATTCTATAGT
CCACTGTCAG
CCATTGTTTT
GGATAGTATT
TATTTTTCTC
TACAAGTATA
GTCAATAGTT
TTCTATTATT
TTAAAGGTTT
GTAACATTTA
AGGGTGACCA
AATGCAAAGT
AAAATTTCAT
TTTCGGGTGA
ACTATCTCGT
TTAACATGGG
AGAAGTGCAA
AACATACATT
ATTGGCTAGA
ACATTGTAGT
ATTTTTTAAA
TGGAAATGTG
TGATTGCTAA
TCTTACTTTG
AATTTGTTTA
CAGGGATCCA
TGGTGAGCAA
GGGCGAGGAG
CTGTTCACCG
GGGTGGTGCC
CATCCTGGTC
GAGCTGGACG
GCGACGTAAA
CGGCCACAAG
TTCAGCGTGT
CCGGCGAGGG
CGAGGGCGAT
GCCACCTACG
GCAAGCTGAC
CCTGAAGTTC
ATCTGCACCA
CCGGCAAGCT
GCCCGTGCCC
TGGCCCACCC
TCGTGACCAC
CCTGACCTAC
GGCGTGCAGT
GCTTCAGCCG
CTACCCCGAC
CACATGAAGC
AGCACGACTT
CTTCAAGTCC
GCCATGCCCG
AAGGCTACGT
CCAGGAGCGC
ACCATCTTCT
TCAAGGACGA
CGGCAACTAC
AAGACCCGCG
CCGAGGTGAA
GTTCGAGGGC
GACACCCTGG
TGAACCGCAT
CGAGCTGAAG
GGCATCGACT
TCAAGGAGGA
CGGCAACATC
CTGGGGCACA
AGCTGGAGTA
CAACTACAAC
AGCCACAACG
TCTATATCAT
GGCCGACAAG
CAGAAGAACG
GCATCAAGGT
GAACTTCAAG
ATCCGCCACA
ACATCGAGGA
CGGCAGCGTG
CAGCTCGCCG
ACCACTACCA
GCAGAACACC
CCCATCGGCG
ACGGCCCCGT
GCTGCTGCCC
GACAACCACT
ACCTGAGCAC
CCAGTCCGCC
CTGAGCAAAG
ACCCCAACGA
GAAGCGCGAT
CACATGGTCC
TGCTGGAGTT
CGTGACCGCC
GCCGGGATCA
CTCTCGGCAT
GGACGAGCTG
TACAAGGGAA
GCGGAGCTAC
TAACTTCAGC
CTGCTGAAGC
AGGCTGGAGA
CGTGGAGGAG
AACCCTGGAC
CTCCCGGGAT
GTCGACGATG
GACTACAGTT
ATGATGCGGA
CCTGGAAGAG
ATGTGTCCGG
TGTGCGGGGA
TAAAGTTTCT
GGCTATCATT
ATGGTTTATT
AACCTGCGAA
AGCTGCAAGG
GTTTCTTTAA
AAGGACCGTA
CAGAATAACA
AACGGTACAC
ATGTGCAGAG
AGTCAGGACT
GTAAGATTGA
TAAAACCCAG
AGGAAACGGT
GTCCTTTCTG
CCGCTTCCAG
AAATGTCTCA
ACGTCGGGAT
GCGACTGGAA
GCTGTCCGTG
CGGATAGGAT
GCGAGGAGGC
CGCAACAAAT
TCGGCCCCAT
GTATAAGCGA
GACCGGGCGT
TAAAGCAGCA
GAAGAAAGCT
CTCATCCGGG
CCAGTGGCTT
AAAGATGGAA
GCCACACCCC
CTTTGCTGAC
GTCACCACAA
CCTGACTACA
CCTTCAGCAC
AGCTTTGTCT
GTGCCAGCTC
CGAAAAACAC
CCATCCGAAC
ATCGTCACCT
CTGTGGCACC
CACCGATTAC
GAACGCAGCC
TCTACGCATC
CAGCTCCCTG
AGTTTATCCG
TCCCCATCCC
GGCCCACACT
CCTCTCCCAG
CACAATACCC
CTATCCAAAT
CTGCCCAGCC
GTGCCATAAA
GTCTGAGTAT
CCTGACCACT
ACACAAGCTC
AGAGCATTAC
ACCAGTGCCA
GCTCCCCAGA
GTCAGTGCCA
GGCTACACGT
ATATCGAGCA
AACACGGGTG
TCCAGCAGCC
CGCAGGTGGT
GGCACCGGGT
TTAACAGTGC
CGCCTTTGGT
TCTGGAGTTT
GTTCGCTGTG
AGCAGGATGA
GCTCCAGGTG
CAAAGCAAAA
TAAGCGCTCA
CCTGGCGCAC
CTGCAGCAGG
AGCAGAACTC
ACGGAGCACG
GCAGCCAATC
AGGAGCAGAG
CTCCCGCCTC
GCAGCCAAAC
CCGAGCGGCT
CAGCACATTT
GGCCTCATGT
GCCACATGGC
CGACCAGACG
CTCTTCTCTA
TCGTGGAGTG
GGCACGAAGC
TGCATCTTCT
TTAAGGAGCT
TAAGGTTGGA
GATCAGATGA
AGTTGCTGCA
TAACTGCTGG
AGTGAACTTC
TAGTGCTGGA
CTATGTTGCC
AGACAGTTGC
ATCATGGGAA
AGAGGACAGT
GTGCTTCTCA
TTACTGGACA
GGAGGTGGAG
CTTGCATCTC
TCCTGGCCCA
GGCAGGGGTC
ACTTTGAGCG
GGATGATCCA
GAGAGGACAG
GAGCTGGTGC
AGCGTCTACA
GGAGCTACAG
CTGGACCGCA
GAGAAACCGC
CTGCCTCAAA
TACCTCATCC
TCTTTAACCC
CGATGTGAAA
CTGTTGGAAA
ACCAGCCTTA
CGTGGAGAGC
GTGTACGAGC
AGGTGAACGC
CGCGCTGTTG
GAGTACACGC
TGTGCGCGTA
TCCGCAATTC
CCGGACAGGT
TTAGCCAGAT
TCTGCTGCGC
CTTCCTGAGC
TCAGAGCGCT
GAGTTCGCAA
GCCGAAGACT
ACCTGTGCTA
CAAACACCTG
AGCGGAGAGG
TGCCCTGCAA
CAACCTCCTC
ATAGAAATGC
TGCATGCCAA
GAGAACCTGC
ATCTGACATA
TGTAAGTATA
CTTGATATCG
ACATGATAAG
ATACATTGAT
GAGTTTGGAC
AAACCACAAC
TAGAATGCAG
TGAAAAAAAT
GCTTTATTTG
TGAAATTTGT
GATGCTATTG
CTTTATTTGT
AACCATTATA
AGCTGCAATA
AACAAGTTAA
CAACAACAAT
TGCATTCATT
TTATGTTTCA
GGTTCAGGGG
GAGGTGTGGG
AGGTTTTTTA
AAGCAAGTAA
AACCTCTACA
AATGTGGTAT
GGCTGATTAT
GATCCTCTAG
ATCAGATCTG
CGAAGATACG
GCCACGGGTG
CTCTTGATCC
TGTGGCTGAT
TTTGGACTGT
GCTGCTCGCA
GCTGCTGATG
AATCACATAC
TTCCTCCATT
TTCTTCCACT
GATTGACTGT
TATAATTTCC
CTAATTTCCA
GGTCAAGGTG
CTGTGCATTG
TGGTAATAGA
TGTGACATGA
CGTCACTTCC
AAAGGACCAA
TGAACATGTC
TGACCAATTT
CATATAATGT
GAAAACGATT
TTCATAGGCA
GAATAAATAA
CATTTAAATT
AAACTGGGCA
TCAGCGCAAT
TCAATTGGTT
TGGTAATAGC
AAGGGAAAAT
AGAATGAAGT
GATCTCCAAA
AAATAAGTAC
TTTTTGACTG
TAAATAAAAT
TGTAAGGAGT
AAAAAGTACT
TTTTTTTCTA
AAAAAATGTA
ATTAAGTAAA
AGTAAAAGTA
TTGATTTTTA
ATTGTACTCA
AGTAAAGTAA
AAATCCCCAA
AAATAATACT
TAAGTACAGT
AATCAAGTAA
AATTACTCAA
GTACTTTACA
CCTCTGGTTC
TTGACCCCCT
ACCTTCAGCA
AGCCCAGCAG
ATCCACTAGT
TCTAGAGCGG
CCGCCACCGC
GGTGGAGCTC
CAGCTTTTGT
TCCCTTTAGT
GAGGGTTAAT
TGCGCGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTG