名称: | pLV3-CMV-Usp16(rat)-3×FLAG-CopGFP-Puro |
---|---|
别称: | NM_001100501.1 |
基因长: | 2475bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 大鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-Usp16(rat)-3×FLAG-CopGFP-Puro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动大鼠源Usp16基因转录,促进Usp16基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带的CopGFP绿色荧光蛋白用于观察目的基因的表达和定位情况。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGG
GAAAGAAACG
AACAAAGGGG
AAAAGTGTTC
CAGAAAAGGC
TTCTTCTGAA
TCTACAGAAC
CAATGTGCAG
ACACCTTAGA
AAAGGGTTGG
AACAAGGTAA
TTTGAAAAAA
GCTTTGGTAA
ATGTGGAATG
GAATATCTGC
CAAGACTGTA
AGACTGACAA
TAAAGTGAAA
GATAAATCTG
AGGAAGAAGC
AGAAGACCCT
TCGGTTTGGC
TGTGTCTTAA
ATGTGGCCAT
CAGGGCTGTG
GCAGAGATTC
CCAGGAGCAG
CATGCCCTGA
AGCACTACAC
AACACCAAGG
TCTGAACCTC
ACTACCTGGT
GCTTAGTTTG
GACAACTGGA
GCGTCTGGTG
TTACAAGTGT
GATGAGGAAA
TCAAGTACTG
CAGTTCAAAC
CGGCTGGGCC
AAGTGGTTGA
TTATGTTAGA
AAGCAAGCTG
GCAGAATAAC
TTCAAAACCA
GAGAAAAATA
ATGGACACAT
TGAGCTTGAA
AATAAAAAAT
TGGAGAAAGA
GAGTAAAAAT
GAACAAGAGA
GAGAAAAATC
GGAAAGCATG
GCTAAAGAAA
ACATCCCCTT
GGACTCTGCT
TCCCAGATAA
CGGTGAAAGG
ACTCAGCAAC
TTGGGGAATA
CTTGTTTCTT
CAACGCTGTT
ATGCAGAACT
TGTCACAAAC
GCCAGTGCTT
AGAGAACTAC
TAAAAGAAGT
GAAGATGTCT
GGAACAATTG
TAAAAATAGA
GCCACCTGAT
CTGGCACTCA
CAGAACCTTT
AGAAGTAAAC
CTTGAACCTC
CAGGTCCTCT
TACTTTAGCC
ATGAGCCAGT
TTCTTAATGA
GATGCAAGAG
AACAAAAAAC
GAATTGTAAC
ACCTAAAGAG
CTCTTTTCTC
AGGTCTGTAA
AAAAGCAACA
CGTTTTAAAG
GGTACCAACA
ACAAGACAGC
CAGGAGCTGC
TTCGCTACCT
ATTGGACGGG
ATGAGAGCGG
AAGAGCACCA
AAGAGTGAGT
AAAGGAATTC
TTAAAGCATT
TGGTAATTCT
ACTGAAAAAT
TGGATGAAGA
AGTAAAAAAT
AAAGTTAAAG
ATTATGAAAA
GAAAAAGGCA
ATCCCAAGTT
TTGTGGACCG
CATCTTCGGT
GGCGAACTGA
CTAGTACAAT
CATGTGTGAT
GACTGCAGGA
CTGTCTCCTT
GGTGCATGAG
TCTTTCCTTG
ATTTGTCTCT
CCCAGTTTTA
GATGATCAGA
GTGGTAAGAA
AAATATAAAT
GATAAAAATG
TGAAGAAGAC
AATGGAGGAA
GAAGATAAAG
ACAGTGAGGA
AGAGAAAGAT
GACAGCTACA
TGAAAACACG
GAGTGACGTG
CCCTCAGGAA
CAAGCAAGCA
CACACAGAAG
AAAGCAAAGA
AGCAAGCCAA
AAAGCAAGCC
AAGAACCAAC
GAAGGCAACA
AAAAATTCAA
GAAAGGTTTC
TTCACTTCAA
CGAGATCTGC
ACCACTAACT
ACACTGAAGA
CAATGACCAT
GAAGCTGAGA
CGGCACTTCC
GGGAGAAGGG
GAAGTGGATA
CTGAGTTCAA
CCGTGGCTCT
CAAGAGGAGC
TCACACAGAC
AGAGCTCTGT
GCTAATCAGA
AAGATGTGAA
TGGCCAAGAG
GAAATGATAG
AAAGCGCTGC
CGATGAGCGG
AAATGCCCAG
AGCACCCGGA
GGTGAAAAGT
GTCAGCACCG
AGAGTGATCT
GGGGAGTTTG
ACTTCTGCTC
CTGAGTGTCC
TAGAGATTTA
AATGGTGCCT
TCCTGGAAGA
AAGGACCAGT
GGAGAGCTAG
ACATTACCAA
TGGTTTAAAA
AACCTTACTT
TGAATGCTGC
TGTTGATCCT
GATGAAATAA
GTATAGAGAT
TCTGAATGAC
AGCCATTCTC
CTGCGTTAAA
GGTGTATGAG
GTCATGAATG
AGGACCCAGA
AACTGCTTTC
TGTACCCTTG
CAAACCGGGA
AGCTTTTAGT
ACTGATGAGT
GTTCAATCCA
ACATTGTTTG
TATCAGTTCA
CCCGCAATGA
GAAACTTCAA
GATGCAAATA
AACTGCTTTG
TGAAGTGTGC
ACAAGACGGC
AGTGTAATGG
ACCAAAGGCA
AATATAAAAG
GTGAAAGGAA
ACATGTTTAC
ACCAATGCCA
AGAAGCAGAT
GCTGGTTTCC
CTTGCTCCTC
CTGTACTCAC
TCTTCATTTA
AAGAGATTTC
AGCAGGCTGG
TTTTAACCTA
CGCAAAGTTA
ACAAACACAT
AAAGTTTCCG
GAAATCTTAG
ATTTGGCTCC
TTTTTGTACC
CTTAAATGTA
AGAACGTTGC
TGAGGAAAGT
ACAAGAGTGC
TGTATTCCTT
ATATGGAGTT
GTGGAACACA
GTGGCACTAT
GAGGTCAGGG
CATTACACTG
CCTACGCAAA
GGAAAGAACT
GCAAGCTGTC
ATCTCTCTAA
TCTTGTTCTT
CATGGTGACA
TTCCGCAAGA
TTGTGAAATG
GAGTCAACCA
AAGGGCAGTG
GTTTCACATC
AGCGACACAC
ACGTGCAAGC
GGTGCCTATA
ACTAAAGTAC
TGAACTCACA
AGCTTATCTC
CTATTTTATG
AGAGAATACT
GGGATCCGAC
TACAAAGACC
ATGACGGTGA
TTATAAAGAT
CATGACATCG
ACTACAAGGA
TGACGATGAC
AAGTAGCGGC
CGCGAAGGAT
CTGCGATCGC
TCCGGTGCCC
GTCAGTGGGC
AGAGCGCACA
TCGCCCACAG
TCCCCGAGAA
GTTGGGGGGA
GGGGTCGGCA
ATTGAACGGG
TGCCTAGAGA
AGGTGGCGCG
GGGTAAACTG
GGAAAGTGAT
GTCGTGTACT
GGCTCCGCCT
TTTTCCCGAG
GGTGGGGGAG
AACCGTATAT
AAGTGCAGTA
GTCGCCGTGA
ACGTTCTTTT
TCGCAACGGG
TTTGCCGCCA
GAACACAGCT
GAAGCTTCGA
GGGGCTCGCA
TCTCTCCTTC
ACGCGCCCGC
CGCCCTACCT
GAGGCCGCCA
TCCACGCCGG
TTGAGTCGCG
TTCTGCCGCC
TCCCGCCTGT
GGTGCCTCCT
GAACTGCGTC
CGCCGTCTAG
GTAAGTTTAA
AGCTCAGGTC
GAGACCGGGC
CTTTGTCCGG
CGCTCCCTTG
GAGCCTACCT
AGACTCAGCC
GGCTCTCCAC
GCTTTGCCTG
ACCCTGCTTG
CTCAACTCTA
CGTCTTTGTT
TCGTTTTCTG
TTCTGCGCCG
TTACAGATCC
AAGCTGTGAC
CGGCGCCTAC
GCTAGACGCC
ACCATGGAGA
GCGACGAGAG
CGGCCTGCCC
GCCATGGAGA
TCGAGTGCCG
CATCACCGGC
ACCCTGAACG
GCGTGGAGTT
CGAGCTGGTG
GGCGGCGGAG
AGGGCACCCC
CAAGCAGGGC
CGCATGACCA
ACAAGATGAA
GAGCACCAAA
GGCGCCCTGA
CCTTCAGCCC
CTACCTGCTG
AGCCACGTGA
TGGGCTACGG
CTTCTACCAC
TTCGGCACCT
ACCCCAGCGG
CTACGAGAAC
CCCTTCCTGC
ACGCCATCAA
CAACGGCGGC
TACACCAACA
CCCGCATCGA
GAAGTACGAG
GACGGCGGCG
TGCTGCACGT
GAGCTTCAGC
TACCGCTACG
AGGCCGGCCG
CGTGATCGGC
GACTTCAAGG
TGGTGGGCAC
CGGCTTCCCC
GAGGACAGCG
TGATCTTCAC
CGACAAGATC
ATCCGCAGCA
ACGCCACCGT
GGAGCACCTG
CACCCCATGG
GCGATAACGT
GCTGGTGGGC
AGCTTCGCCC
GCACCTTCAG
CCTGCGCGAC
GGCGGCTACT
ACAGCTTCGT
GGTGGACAGC
CACATGCACT
TCAAGAGCGC
CATCCACCCC
AGCATCCTGC
AGAACGGGGG
CCCCATGTTC
GCCTTCCGCC
GCGTGGAGGA
GCTGCACAGC
AACACCGAGC
TGGGCATCGT
GGAGTACCAG
CACGCCTTCA
AGACCCCCAT
CGCCTTCGCC
AGATCCCGCG
CTCAGTCGTC
CAATTCTGCC
GTGGACGGCA
CCGCCGGACC
CGGCTCCACC
GGATCTCGCG
AGGGCAGAGG
AAGTCTTCTA
ACATGCGGTG
ACGTGGAGGA
GAATCCCGGC
CCTATGACCG
AGTACAAGCC
CACGGTGCGC
CTCGCCACCC
GCGACGACGT
CCCCAGGGCC
GTACGCACCC
TCGCCGCCGC
GTTCGCCGAC
TACCCCGCCA
CGCGCCACAC
CGTCGATCCG
GACCGCCACA
TCGAGCGGGT
CACCGAGCTG
CAAGAACTCT
TCCTCACGCG
CGTCGGGCTC
GACATCGGCA
AGGTGTGGGT
CGCGGACGAC
GGCGCCGCGG
TGGCGGTCTG
GACCACGCCG
GAGAGCGTCG
AAGCGGGGGC
GGTGTTCGCC
GAGATCGGCC
CGCGCATGGC
CGAGTTGAGC
GGTTCCCGGC
TGGCCGCGCA
GCAACAGATG
GAAGGCCTCC
TGGCGCCGCA
CCGGCCCAAG
GAGCCCGCGT
GGTTCCTGGC
CACCGTCGGC
GTCTCGCCCG
ACCACCAGGG
CAAGGGTCTG
GGCAGCGCCG
TCGTGCTCCC
CGGAGTGGAG
GCGGCCGAGC
GCGCCGGGGT
GCCCGCCTTC
CTGGAGACCT
CCGCGCCCCG
CAACCTCCCC
TTCTACGAGC
GGCTCGGCTT
CACCGTCACC
GCCGACGTCG
AGGTGCCCGA
AGGACCGCGC
ACCTGGTGCA
TGACCCGCAA
GCCCGGTGCC
TGAAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCCTGGTACC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGATAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TAGTAGTTCA
TGTCATCTTA
TTATTCAGTA
TTTATAACTT
GCAAAGAAAT
GAATATCAGA
GAGTGAGAGG
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGCTCTAGCT
ATCCCGCCCC
TAACTCCGCC
CATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
GGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
ACTTTTGCAG
AGACCAAATT
CGTAATCATG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGGACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCTAA
GAAACCATTA
TTATCATGAC
ATTAACCTAT
AAAAATAGGC
GTATCACGAG
GCCCTTTCGT
CTCGCGCGTT
TCGGTGATGA
CGGTGAAAAC
CTCTGACACA
TGCAGCTCCC
GGAGACGGTC
ACAGCTTGTC
TGTAAGCGGA
TGCCGGGAGC
AGACAAGCCC
GTCAGGGCGC
GTCAGCGGGT
GTTGGCGGGT
GTCGGGGCTG
GCTTAACTAT
GCGGCATCAG
AGCAGATTGT
ACTGAGAGTG
CACCATATGC
GGTGTGAAAT
ACCGCACAGA
TGCGTAAGGA
GAAAATACCG
CATCAGGCGC
CATTCGCCAT
TCAGGCTGCG
CAACTGTTGG
GAAGGGCGAT
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGC
TGGCGAAAGG
GGGATGTGCT
GCAAGGCGAT
TAAGTTGGGT
AACGCCAGGG
TTTTCCCAGT
CACGACGTTG
TAAAACGACG
GCCAGTGCCA
AGCTG