名称: | pLVX-CMV-Galpha-i2 ONE-GO |
---|---|
别称: | Plasmid #189736 |
基因长: | 1791bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 大鼠 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | Nluc |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLVX-IRES-Gαi*-BERKY3 |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLVX-CMV-Galpha-i2 ONE-GO是适用于哺乳细胞过表达的质粒。CMV增强子和CMV启动子联合启动大鼠源Galpha-i2 ONE-GO基因转录。质粒携带Nluc荧光标记,可直观反映 Nluc 基因的表达水平,进而间接反映目的基因的表达情况。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGACTC
TACTAGAGGA
TCTATTTCCG
GTGAATTCCT
CGAGACTAGT
TCTAGAGCGG
CCGCGGATCC
ATGGGATGCA
CCGTTAGCGC
CGAAGACAAA
GCAGCGGCGG
AGCGAAGCAA
GATGATTGAC
AAAAATTTGA
GAGAGGATGG
CGAGAAGGCG
GCTCGCGAGG
TCAAACTGTT
GCTTCTGGGT
GCCGGAGAGA
GCGGCAAGAG
CACTATTGTA
AAACAGATGA
AGATCATTCA
TGAGGATGGC
TACTCAGAGG
AGGAATGTCG
GCAATACCGC
GCAGTTGTGT
ATAGTAATAC
TATTCAATCC
ATTATGGCAA
TTGTAAAGGC
GATGGGCAAC
CTCCAGATCG
ACTTTGCTGA
CCCGCAGAGG
GCAGACGACG
CGAGGCAGCT
GTTCGCCTTG
AGCTGCGCCG
CTATGGTGAG
CAAGGGCGAG
GAGCTGTTCA
CCGGGGTGGT
GCCCATCCTG
GTCGAGCTGG
ACGGCGACGT
AAACGGCCAC
AAGTTCAGCG
TGTCCGGCGA
GGGCGAGGGC
GATGCCACCT
ACGGCAAGCT
GACCCTGAAG
TTCATCTGCA
CCACCGGCAA
GCTGCCCGTG
CCCTGGCCCA
CCCTCGTGAC
CACCTTCGGC
TACGGCCTGA
TGTGCTTCGC
CCGCTACCCC
GACCACATGA
AGCAGCACGA
CTTCTTCAAG
TCCGCCATGC
CCGAAGGCTA
CGTCCAGGAG
CGCACCATCT
TCTTCAAGGA
CGACGGCAAC
TACAAGACCC
GCGCCGAGGT
GAAGTTCGAG
GGCGACACCC
TGGTGAACCG
CATCGAGCTG
AAGGGCATCG
ACTTCAAGGA
GGACGGCAAC
ATCCTGGGGC
ACAAGCTGGA
GTACAACTAC
AACAGCCACA
ACGTCTATAT
CATGGCCGAC
AAGCAGAAGA
ACGGCATCAA
GGTGAACTTC
AAGATCCGCC
ACAACATCGA
GGACGGCAGC
GTGCAGCTCG
CCGACCACTA
CCAGCAGAAC
ACCCCCATCG
GCGACGGCCC
CGTGTTGCTG
CCCGACAACC
ACTACCTGAG
CTACCAGTCC
GCCCTGAGCA
AAGACCCCAA
CGAGAAGCGC
GATCACATGG
TCCTGCTGGA
GTTCGTGACC
GCCGCCGGGA
TCACTCTCGG
CATGGACGAG
CTGTACAAGA
GCGGCGAGGA
ACAAGGGATG
TTGCCGGAAG
ATCTGTCAGG
AGTGATTCGG
CGGCTGTGGG
CCGACCATGG
AGTGCAAGCG
TGCTTTGGGA
GGAGCCGGGA
ATACCAACTG
AACGATTCCG
CCGCTTATTA
CTTGAACGAC
CTTGAGCGCA
TTGCTCAAAG
CGATTATATT
CCAACTCAAC
AAGATGTACT
GAGAACACGC
GTGAAGACAA
CAGGAATAGT
AGAGACTCAT
TTCACGTTTA
AGGATCTCCA
CTTTAAAATG
TTCGATGTTG
GAGGACAACG
ATCTGAGCGC
AAGAAGTGGA
TACACTGTTT
TGAGGGCGTT
ACCGCGATCA
TTTTCTGTGT
TGCTTTGTCT
GCATACGACC
TCGTCTTGGC
AGAGGATGAA
GAAATGAATC
GAATGCACGA
ATCCATGAAG
TTGTTTGATT
CTATATGCAA
CAATAAGTGG
TTTACTGATA
CCAGCATCAT
ACTTTTCTTG
AATAAAAAAG
ACTTGTTTGA
GGAGAAGATA
ACACAGAGCC
CACTTACGAT
TTGTTTCCCG
GAGTATACGG
GAGCGAACAA
GTATGATGAA
GCCGCAAGTT
ATATCCAATC
CAAGTTTGAG
GACCTGAATA
AGAGAAAAGA
CACAAAGGAG
ATTTACACAC
ACTTCACCTG
TGCCACTGAC
ACTAAAAACG
TCCAATTTGT
TTTCGACGCC
GTTACCGATG
TCATAATAAA
AAATAACCTC
AAAGACTGTG
GTCTTTTTTA
AGCGGCCGCC
GCCCCTCTCC
CTCCCCCCCC
CCTAACGTTA
CTGGCCGAAG
CCGCTTGGAA
TAAGGCCGGT
GTGCGTTTGT
CTATATGTTA
TTTTCCACCA
TATTGCCGTC
TTTTGGCAAT
GTGAGGGCCC
GGAAACCTGG
CCCTGTCTTC
TTGACGAGCA
TTCCTAGGGG
TCTTTCCCCT
CTCGCCAAAG
GAATGCAAGG
TCTGTTGAAT
GTCGTGAAGG
AAGCAGTTCC
TCTGGAAGCT
TCTTGAAGAC
AAACAACGTC
TGTAGCGACC
CTTTGCAGGC
AGCGGAACCC
CCCACCTGGC
GACAGGTGCC
TCTGCGGCCA
AAAGCCACGT
GTATAAGATA
CACCTGCAAA
GGCGGCACAA
CCCCAGTGCC
ACGTTGTGAG
TTGGATAGTT
GTGGAAAGAG
TCAAATGGCT
CTCCTCAAGC
GTATTCAACA
AGGGGCTGAA
GGATGCCCAG
AAGGTACCCC
ATTGTATGGG
ATCTGATCTG
GGGCCTCGGT
GCACATGCTT
TACATGTGTT
TAGTCGAGGT
TAAAAAAACG
TCTAGGCCCC
CCGAACCACG
GGGACGTGGT
TTTCCTTTGA
AAAACACGAT
GATAAGCTTG
CCACAACCCG
TACCAAAGAT
GGGGAGTAGC
AAGAGCAAGA
CGTCGAATTC
CGGAATCAAG
CTTGGTGGAT
CTTCTCGAGG
TTACTACCAT
GGTATTTGGG
TGGGTGAAGA
AGGTCGACTT
TCTCGAGGTG
GCACCGGTGG
ATCCATGGTC
TTCACACTCG
AAGATTTCGT
TGGGGACTGG
CGACAGACAG
CCGGCTACAA
CCTGGACCAA
GTCCTTGAAC
AGGGAGGTGT
GTCCAGTTTG
TTTCAGAATC
TCGGGGTGTC
CGTAACTCCG
ATCCAAAGGA
TTGTCCTGAG
CGGTGAAAAT
GGGCTGAAGA
TCGACATCCA
TGTCATCATC
CCGTATGAAG
GTCTGAGCGG
CGACCAAATG
GGCCAGATCG
AAAAAATTTT
TAAGGTGGTG
TACCCTGTGG
ATGATCATCA
CTTTAAGGTG
ATCCTGCACT
ATGGCACACT
GGTAATCGAC
GGGGTTACGC
CGAACATGAT
CGACTATTTC
GGACGGCCGT
ATGAAGGCAT
CGCCGTGTTC
GACGGCAAAA
AGATCACTGT
AACAGGGACC
CTGTGGAACG
GCAACAAAAT
TATCGACGAG
CGCCTGATCA
ACCCCGACGG
CTCCCTGCTG
TTCCGAGTAA
CCATCAACGG
AGTGACCGGC
TGGCGGCTGT
GCGAACGCAT
TCTGGCGTAA
ACGCGTCTGG
AACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAGC
TGACGTCCTT
TCCATGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCCTGGAATT
AATTCTGCAG
TCGAGACCTA
GAAAAACATG
GAGCAATCAC
AAGTAGCAAT
ACAGCAGCTA
CCAATGCTGA
TTGTGCCTGG
CTAGAAGCAC
AAGAGGAGGA
GGAGGTGGGT
TTTCCAGTCA
CACCTCAGGT
ACCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GAGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGACAA
GATATCCTTG
ATCTGTGGAT
CTACCACACA
CAAGGCTACT
TCCCTGATTA
GCAGAACTAC
ACACCAGGGC
CAGGGGTCAG
ATATCCACTG
ACCTTTGGAT
GGTGCTACAA
GCTAGTACCA
GTTGAGCCAG
ATAAGGTAGA
AGAGGCCAAT
AAAGGAGAGA
ACACCAGCTT
GTTACACCCT
GTGAGCCTGC
ATGGGATGGA
TGACCCGGAG
AGAGAAGTGT
TAGAGTGGAG
GTTTGACAGC
CGCCTAGCAT
TTCATCACGT
GGCCCGAGAG
CTGCATCCGG
AGTACTTCAA
GAACTGCTGA
TATCGAGCTT
GCTACAAGGG
ACTTTCCGCT
GGGGACTTTC
CAGGGAGGCG
TGGCCTGGGC
GGGACTGGGG
AGTGGCGAGC
CCTCAGATCC
TGCATATAAG
CAGCTGCTTT
TTGCCTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTAGTAGT
TCATGTCATC
TTATTATTCA
GTATTTATAA
CTTGCAAAGA
AATGAATATC
AGAGAGTGAG
AGGCCTTGAC
ATTGCTAGCG
TTTACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCGAC
GGATCGGGAG
ATCAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGGATCAA
CTGGATAACT
CAAGCTAACC
AAAATCATCC
CAAACTTCCC
ACCCCATACC
CTATTACCAC
TGCCAATTAC
CTGTGGTTTC
ATTTACTCTA
AACCTGTGAT
TCCTCTGAAT
TATTTTCATT
TTAAAGAAAT
TGTATTTGTT
AAATATGTAC
TACAAACTTA
GTAGTTGGAA
GGGCTAATTC
ACTCCCAAAG
AAGACAAGAT
ATCCTTGATC
TGTGGATCTA
CCACACACAA
GGCTACTTCC
CTGATTAGCA
GAACTACACA
CCAGGGCCAG
GGGTCAGATA
TCCACTGACC
TTTGGATGGT
GCTACAAGCT
AGTACCAGTT
GAGCCAGATA
AGGTAGAAGA
GGCCAATAAA
GGAGAGAACA
CCAGCTTGTT
ACACCCTGTG
AGCCTGCATG
GGATGGATGA
CCCGGAGAGA
GAAGTGTTAG
AGTGGAGGTT
TGACAGCCGC
CTAGCATTTC
ATCACGTGGC
CCGAGAGCTG
CATCCGGAGT
ACTTCAAGAA
CTGCTGATAT
CGAGCTTGCT
ACAAGGGACT
TTCCGCTGGG
GACTTTCCAG
GGAGGCGTGG
CCTGGGCGGG
ACTGGGGAGT
GGCGAGCCCT
CAGATCCTGC
ATATAAGCAG
CTGCTTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TGGCGCCCGA
ACAGGGACTT
GAAAGCGAAA
GGGAAACCAG
AGGAGCTCTC
TCGACGCAGG
ACTCGGCTTG
CTGAAGCGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGTACGCCA
AAAATTTTGA
CTAGCGGAGG
CTAGAAGGAG
AGAGATGGGT
GCGAGAGCGT
CAGTATTAAG
CGGGGGAGAA
TTAGATCGCG
ATGGGAAAAA
ATTCGGTTAA
GGCCAGGGGG
AAAGAAAAAA
TATAAATTAA
AACATATAGT
ATGGGCAAGC
AGGGAGCTAG
AACGATTCGC
AGTTAATCCT
GGCCTGTTAG
AAACATCAGA
AGGCTGTAGA
CAAATACTGG
GACAGCTACA
ACCATCCCTT
CAGACAGGAT
CAGAAGAACT
TAGATCATTA
TATAATACAG
TAGCAACCCT
CTATTGTGTG
CATCAAAGGA
TAGAGATAAA
AGACACCAAG
GAAGCTTTAG
ACAAGATAGA
GGAAGAGCAA
AACAAAAGTA
AGACCACCGC
ACAGCAAGCG
GCCGGCCGCT
GATCTTCAGA
CCTGGAGGAG
GAGATATGAG
GGACAATTGG
AGAAGTGAAT
TATATAAATA
TAAAGTAGTA
AAAATTGAAC
CATTAGGAGT
AGCACCCACC
AAGGCAAAGA
GAAGAGTGGT
GCAGAGAGAA
AAAAGAGCAG
TGGGAATAGG
AGCTTTGTTC
CTTGGGTTCT
TGGGAGCAGC
AGGAAGCACT
ATGGGCGCAG
CGTCAATGAC
GCTGACGGTA
CAGGCCAGAC
AATTATTGTC
TGGTATAGTG
CAGCAGCAGA
ACAATTTGCT
GAGGGCTATT
GAGGCGCAAC
AGCATCTGTT
GCAACTCACA
GTCTGGGGCA
TCAAGCAGCT
CCAGGCAAGA
ATCCTGGCTG
TGGAAAGATA
CCTAAAGGAT
CAACAGCTCC
TGGGGATTTG
GGGTTGCTCT
GGAAAACTCA
TTTGCACCAC
TGCTGTGCCT
TGGAATGCTA
GTTGGAGTAA
TAAATCTCTG
GAACAGATTT
GGAATCACAC
GACCTGGATG
GAGTGGGACA
GAGAAATTAA
CAATTACACA
AGCTTAATAC
ACTCCTTAAT
TGAAGAATCG
CAAAACCAGC
AAGAAAAGAA
TGAACAAGAA
TTATTGGAAT
TAGATAAATG
GGCAAGTTTG
TGGAATTGGT
TTAACATAAC
AAATTGGCTG
TGGTATATAA
AATTATTCAT
AATGATAGTA
GGAGGCTTGG
TAGGTTTAAG
AATAGTTTTT
GCTGTACTTT
CTATAGTGAA
TAGAGTTAGG
CAGGGATATT
CACCATTATC
GTTTCAGACC
CACCTCCCAA
CCCCGAGGGG
ACCCGACAGG
CCCGAAGGAA
TAGAAGAAGA
AGGTGGAGAG
AGAGACAGAG
ACAGATCCAT
TCGATTAGTG
AACGGATCTC
GACGGTATCG
CCTTTAAAAG
AAAAGGGGGG
ATTGGGGGGT
ACAGTGCAGG
GGAAAGAATA
GTAGACATAA
TAGCAACAGA
CATACAAACT
AAAGAATTAC
AAAAACAAAT
TACAAAAATT
CAAAATTTTC
GGGTTTATTA
CAGGGACAGC
AGAGATCCAG
TTTATCGATA
AGCTTGGGAG
TTCCG