产品货号：P71407
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV2-CMV-Stag2(mouse)-Puro |
|---|---|
| 别称: | NM_021465.4;9230105L23Rik; B230112I07Rik; SA-2; SAP2; |
| 基因长: | 3696bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 小鼠 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLVX-Puro |
| 正向引物: | CMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV2-CMV-Stag2(mouse)-Puro是适用于哺乳细胞过表达的质粒。CMV增强子和CMV启动子联合启动小鼠源Stag2基因转录。此外，质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装，便于基因稳定整合至宿主细胞。质粒携带Puro抗性基因，用于筛选表达目的基因的宿主细胞。质粒携带Puro抗性基因，用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATAAGCTT
GGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGACTC
TACTAGAGGA
TCGCTAGCGC
TACCGGACTC
AGATCTCGAG
CTCAAGCTTC
GAATTCGCCA
CCATGATAGC
AGCTCCAGAA
ATACCAACTG
ATTTTAATCT
ACTGCAGGAG
TCAGAGACAC
ACTTTTCTTC
TGACACAGAT
TTTGAAGACA
TTGAAGGAAA
AAATCAGAAG
CAAGGCAAAG
GCAAAACTTG
TAAAAAAGGC
AAAAAGGGTC
CAGCAGAAAA
GGGTAAAAGT
GGAAATGGAG
GAGGAAAACC
TCCTTCTGGC
TCAAACCGAA
TGAACGGTCA
TCATCAACAG
AATGGAGTTG
AAAACATGAT
GTTGTTCGAA
GTTGTTAAAA
TGGGCAAGAG
TGCTATGCAG
TCGGTGGTAG
ATGACTGGAT
CGAGTCCTAC
AAGCATGACC
GGGACATAGC
GCTTCTTGAC
CTTATCAATT
TCTTTATTCA
GTGCTCAGGC
TGCAAAGGAG
TCGTCACAGC
AGAGATGTTC
AGACATATGC
AGAACTCTGA
GATAATTCGA
AAAATGACAG
AAGAATTTGA
CGAGGATAGT
GGGGATTACC
CACTTACCAT
GGCTGGTCCT
CAGTGGAAGA
AGTTCAAGTC
CAGCTTCTGT
GAGTTCATTG
GTGTGTTAGT
ACGGCAGTGT
CAATACAGTA
TCATATATGA
TGAGTACATG
ATGGATACTG
TCATTTCACT
TCTTACTGGA
TTGTCTGACT
CCCAAGTCAG
AGCATTTCGA
CATACAAGCA
CCCTGGCAGC
TATGAAGTTG
ATGACTGCTT
TGGTAAATGT
GGCACTAAAT
CTTAGCATTA
ATATGGATAA
CACACAAAGA
CAGTACGAGG
CAGAACGGAA
TAAAATGATT
GGAAAACGAG
CTAACGAAAG
GCTAGAACTC
CTGTTACAAA
AGCGTAAAGA
GCTTCAGGAA
AATCAAGATG
AGATAGAAAA
TATGATGAAT
GCAATATTTA
AAGGAGTGTT
TGTACATAGA
TACAGGGATG
CAATAGCTGA
AATCAGAGCT
ATATGCATTG
AAGAGATTGG
CATTTGGATG
AAGATGTATA
GTGATGCCTT
CCTTAATGAC
AGCTATTTAA
AATATGTTGG
TTGGACTATG
CATGATAAGC
AAGGTGAAGT
CAGACTCAAA
TGTCTTACTG
CTTTACAAGG
GCTTTACTAT
AACAAAGAAC
TTAATTCCAA
ACTGGAACTC
TTTACCAGTC
GATTCAAGGA
TAGAATTGTG
TCTATGACCC
TTGACAAAGA
ATATGATGTT
GCAGTACAAG
CAATAAAGTT
ACTCACTCTT
GTTTTACAGA
GTAGTGAAGA
AGTTCTAACT
GCAGAAGATT
GTGAAAATGT
CTATCATCTG
GTTTATTCAG
CTCACCGGCC
AGTAGCAGTT
GCAGCTGGAG
AATTTCTCTA
CAAAAAGCTC
TTCAGCCGCA
GAGACCCAGA
AGAGGATGGG
CTGATGAAAA
GAAGGGGAAG
GCAAGGCCCG
AATGCCAACC
TTGTGAAGAC
TTTGGTGTTT
TTCTTTTTGG
AAAGTGAGTT
ACACGAGCAT
GCAGCTTACC
TTGTGGACAG
CATGTGGGAT
TGTGCTACTG
AGCTCCTGAA
AGACTGGGAA
TGTATGAATA
GCTTGTTATT
AGAAGAGCCT
CTTAGTGGAG
AGGAAGCTCT
AACAGACAGA
CAAGAGAGTG
CTTTGATTGA
AATAATGCTT
TGTACTATTA
GACAAGCAGC
TGAATGTCAT
CCTCCCGTGG
GAAGAGGGAC
AGGAAAAAGG
GTGCTGACAG
CAAAGGAGAA
GAAGACACAG
TTGGATGACA
GAACAAGAAT
CACTGAGCTG
TTTGCTGTGG
CCCTTCCGCA
GTTATTAGCT
AAATACTCTG
TAGATGCAGA
AAAGGTGACC
AATTTGTTGC
AGTTACCTCA
GTACTTTGAT
TTGGAGATAT
ACACCACTGG
ACGATTAGAA
AAGCATTTGG
ATGCCTTATT
GCGCCAGATC
CGAAATATTG
TAGAGAAGCA
CACAGACACA
GATGTTTTGG
AAGCATGTTC
TAAAACTTAC
CATGCATTAT
GTAACGAAGA
GTTCACCATC
TTCAACAGAG
TAGATATTTC
AAGAAGTCAG
CTGATTGATG
AACTGGCAGA
TAAATTTAAC
CGGCTTCTTG
AAGATTTTCT
GCAAGAGGGA
GAAGAACCTG
ATGAAGATGA
CGCATATCAG
GTATTGTCAA
CATTGAAGAG
GATCACTGCT
TTTCATAATG
CTCATGATCT
TTCAAAGTGG
GATTTGTTCG
CTTGTAATTA
CAAACTCTTG
AAAACCGGAA
TCGAAAATGG
AGATATGCCT
GAGCAGATTG
TTATTCACGC
ACTGCAGTGT
GCTCACTATG
TAATCCTTTG
GCAGCTTGCT
AAGATCACTG
AAAGCACGTC
TACAAAGGAG
GACTTACTGC
GCTTAAAGAA
ACAGATGAGG
GTATTTTGCC
AGATATGTCA
GCATTACTTG
ACCAACGTGA
ATACTACTGT
TAAAGAACAG
GCCTTCACTA
TTCTGTGTGA
CATTTTGATG
ATCTTCAGTC
ACCAGATTAT
GTCTGGAGGG
CGTGACATGT
TAGAGCCATT
AGTTTATACT
CCTGATTCTT
CATTGCAGTC
TGAGTTGCTC
AGCTTTATTT
TGGATCATGT
CTTCATTGAA
CAGGATGATG
ATAGTAATAG
TGCAGATGGT
CAGCAAGAGG
ATGAAGCCAG
CAAAATTGAA
GCTTTGCACA
AGAGGAGAAA
TTTACTTGCA
GCATTTTGTA
AGCTAATTGT
ATATACCGTG
GTGGAGATGA
ACACAGCTGC
AGATATCTTC
AAGCAGTATA
TGAAGTATTA
TAATGATTAT
GGCGATATCA
TCAAAGAAAC
AATGAGTAAA
ACAAGGCAGA
TTGACAAAAT
TCAGTGTGCT
AAGACCCTTA
TTCTCAGTTT
GCAGCAGCTT
TTTAATGAGA
TGATACAAGA
GAATGGCTAT
AATTTTGATA
GATCATCCTC
AACATTTAGT
GGCATAAAAG
AACTTGCTCG
GCGTTTTGCT
TTAACTTTTG
GACTTGATCA
ATTGAAAACC
AGAGAAGCCA
TTGCCATGCT
CCACAAAGAT
GGTATAGAAT
TTGCTTTTAA
GGAGCCTAAT
CCACAAGGGG
AGAGCCATCC
ACCTTTAAAC
TTGGCATTTC
TTGATATTCT
CAGTGAATTT
TCTTCTAAAC
TGCTCCGACA
GGATAAAAGA
ACAGTGTATG
TTTATTTGGA
AAAGTTCATG
ACCTTTCAGA
TGTCACTCCG
AAGAGAAGAT
GTATGGCTTC
CTCTGATGTC
TTACCGAAAT
TCTTTGCTAG
CTGGTGGTGA
TGATGATACT
ATGTCAGTCA
TTAGTGGAAT
GAGCAGTCGG
GGATCAACTG
TACGGAGTAA
AAAATCAAAA
CCATCTACAG
GAAAGCGGAA
AGTGGTTGAA
GGCATGCAAC
TAGCACTCCC
TGAAGAAAGC
AGTAGCAGTG
ACAGTATGTG
GTTGAGCAGA
GAGCAAACAC
TGCACACCCC
TGTCATGATG
CAGACACCAC
AGCTCACCTC
CACTATTATG
AGAGAACCGA
AAAGGTTACG
GCCTGAAGAT
AGCTTCATGA
GTGTCTATCC
GATGCAGGCA
GAACACCATC
AAACGCCTCT
GGATTATAAT
CGGCGTGGTA
CAAGCCTAAT
GGAAGATGAT
GAAGAGCCAA
TTGTTGAAGA
TGTTATGATG
TCCTCAGAAG
GAAGGATTGA
GGATCTTAAT
GAGGGAATGG
ACTTTGACAC
CATGGATATA
GACTTGCCAC
CATCAAAGAA
TAGAAGAGAG
AGAACAGAAC
TGAAGCCCGA
TTTCTTTGAT
CCAGCTTCAA
TTATGGATGA
ATCAGTTCTT
GGGGTGTCAA
TGTTTTAATA
ATCTAGATAA
TTCTACCGGG
TAGGGGAGGC
GCTTTTCCCA
AGGCAGTCTG
GAGCATGCGC
TTTAGCAGCC
CCGCTGGGCA
CTTGGCGCTA
CACAAGTGGC
CTCTGGCCTC
GCACACATTC
CACATCCACC
GGTAGGCGCC
AACCGGCTCC
GTTCTTTGGT
GGCCCCTTCG
CGCCACCTTC
TACTCCTCCC
CTAGTCAGGA
AGTTCCCCCC
CGCCCCGCAG
CTCGCGTCGT
GCAGGACGTG
ACAAATGGAA
GTAGCACGTC
TCACTAGTCT
CGTGCAGATG
GACAGCACCG
CTGAGCAATG
GAAGCGGGTA
GGCCTTTGGG
GCAGCGGCCA
ATAGCAGCTT
TGCTCCTTCG
CTTTCTGGGC
TCAGAGGCTG
GGAAGGGGTG
GGTCCGGGGG
CGGGCTCAGG
GGCGGGCTCA
GGGGCGGGGC
GGGCGCCCGA
AGGTCCTCCG
GAGGCCCGGC
ATTCTGCACG
CTTCAAAAGC
GCACGTCTGC
CGCGCTGTTC
TCCTCTTCCT
CATCTCCGGG
CCTTTCGACC
TGCAGCCCAA
GCTTACCATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCAG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
TCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGCGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTGACCG
CGTCTGGAAC
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAGCTGA
CGTCCTTTCC
ATGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGGAATTAAT
TCTGCAGTCG
AGACCTAGAA
AAACATGGAG
CAATCACAAG
TAGCAATACA
GCAGCTACCA
ATGCTGATTG
TGCCTGGCTA
GAAGCACAAG
AGGAGGAGGA
GGTGGGTTTT
CCAGTCACAC
CTCAGGTACC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGAG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGACAAGAT
ATCCTTGATC
TGTGGATCTA
CCACACACAA
GGCTACTTCC
CTGATTAGCA
GAACTACACA
CCAGGGCCAG
GGGTCAGATA
TCCACTGACC
TTTGGATGGT
GCTACAAGCT
AGTACCAGTT
GAGCCAGATA
AGGTAGAAGA
GGCCAATAAA
GGAGAGAACA
CCAGCTTGTT
ACACCCTGTG
AGCCTGCATG
GGATGGATGA
CCCGGAGAGA
GAAGTGTTAG
AGTGGAGGTT
TGACAGCCGC
CTAGCATTTC
ATCACGTGGC
CCGAGAGCTG
CATCCGGAGT
ACTTCAAGAA
CTGCTGATAT
CGAGCTTGCT
ACAAGGGACT
TTCCGCTGGG
GACTTTCCAG
GGAGGCGTGG
CCTGGGCGGG
ACTGGGGAGT
GGCGAGCCCT
CAGATCCTGC
ATATAAGCAG
CTGCTTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TAGTAGTTCA
TGTCATCTTA
TTATTCAGTA
TTTATAACTT
GCAAAGAAAT
GAATATCAGA
GAGTGAGAGG
CCTTGACATT
GCTAGCGTTT
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
AACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTCGACGG
ATCGGGAGAT
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGGATCAACT
GGATAACTCA
AGCTAACCAA
AATCATCCCA
AACTTCCCAC
CCCATACCCT
ATTACCACTG
CCAATTACCT
GTGGTTTCAT
TTACTCTAAA
CCTGTGATTC
CTCTGAATTA
TTTTCATTTT
AAAGAAATTG
TATTTGTTAA
ATATGTACTA
CAAACTTAGT
AGTTTTTAAA
GAAATTGTAT
TTGTTAAATA
TGTACTACAA
ACTTAGTAGT
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