产品货号：P71350
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV3-CMV-KIF11(human)-3×HA-Fluc-Puro |
|---|---|
| 别称: | NM_004523.4;EG5; HKSP; KNSL1; MCLMR; TRIP5 |
| 基因长: | 3168bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | Fluc |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLV3-CMV-MCS-Fluc-Puro |
| 正向引物: | CMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-KIF11(human)-3×HA-Fluc-Puro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动人源KIF11基因转录。促进KIF11基因C端融合表达3×HA标签，便于操作蛋白免疫沉淀、蛋白共免疫沉淀等实验。质粒携带的Fluc荧光标签由EF1a启动子启动转录，直观地反映 Fluc 基因的表达水平，进而间接反映目的基因的表达情况。此外，质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装，便于基因稳定整合至宿主细胞。质粒携带Puro抗性基因，用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTCTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCTAGCG
AATTCGCCAC
CATGGCGTCG
CAGCCAAATT
CGTCTGCGAA
GAAGAAAGAG
GAGAAGGGGA
AGAACATCCA
GGTGGTGGTG
AGATGCAGAC
CATTTAATTT
GGCAGAGCGG
AAAGCTAGCG
CCCATTCAAT
AGTAGAATGT
GATCCTGTAC
GAAAAGAAGT
TAGTGTACGA
ACTGGAGGAT
TGGCTGACAA
GAGCTCAAGG
AAAACATACA
CTTTTGATAT
GGTGTTTGGA
GCATCTACTA
AACAGATTGA
TGTTTACCGA
AGTGTTGTTT
GTCCAATTCT
GGATGAAGTT
ATTATGGGCT
ATAATTGCAC
TATCTTTGCG
TATGGCCAAA
CTGGCACTGG
AAAAACTTTT
ACAATGGAAG
GTGAAAGGTC
ACCTAATGAA
GAGTATACCT
GGGAAGAGGA
TCCCTTGGCT
GGTATAATTC
CACGTACCCT
TCATCAAATT
TTTGAGAAAC
TTACTGATAA
TGGTACTGAA
TTTTCAGTCA
AAGTGTCTCT
GTTGGAGATC
TATAATGAAG
AGCTTTTTGA
TCTTCTTAAT
CCATCATCTG
ATGTTTCTGA
GAGACTACAG
ATGTTTGATG
ATCCCCGTAA
CAAGAGAGGA
GTGATAATTA
AAGGTTTAGA
AGAAATTACA
GTACACAACA
AGGATGAAGT
CTATCAAATT
TTAGAAAAGG
GGGCAGCAAA
AAGGACAACT
GCAGCTACTC
TGATGAATGC
ATACTCTAGT
CGTTCCCACT
CAGTTTTCTC
TGTTACAATA
CATATGAAAG
AAACTACGAT
TGATGGAGAA
GAGCTTGTTA
AAATCGGAAA
GTTGAACTTG
GTTGATCTTG
CAGGAAGTGA
AAACATTGGC
CGTTCTGGAG
CTGTTGATAA
GAGAGCTCGG
GAAGCTGGAA
ATATAAATCA
ATCCCTGTTG
ACTTTGGGAA
GGGTCATTAC
TGCCCTTGTA
GAAAGAACAC
CTCATGTTCC
TTATCGAGAA
TCTAAACTAA
CTAGAATCCT
CCAGGATTCT
CTTGGAGGGC
GTACAAGAAC
ATCTATAATT
GCAACAATTT
CTCCTGCATC
TCTCAATCTT
GAGGAAACTC
TGAGTACATT
GGAATATGCT
CATAGAGCAA
AGAACATATT
GAATAAGCCT
GAAGTGAATC
AGAAACTCAC
CAAAAAAGCT
CTTATTAAGG
AGTATACGGA
GGAGATAGAA
CGTTTAAAAC
GAGATCTTGC
TGCAGCCCGT
GAGAAAAATG
GAGTGTATAT
TTCTGAAGAA
AATTTTAGAG
TCATGAGTGG
AAAATTAACT
GTTCAAGAAG
AGCAGATTGT
AGAATTGATT
GAAAAAATTG
GTGCTGTTGA
GGAGGAGCTG
AATAGGGTTA
CAGAGTTGTT
TATGGATAAT
AAAAATGAAC
TTGACCAGTG
TAAATCTGAC
CTGCAAAATA
AAACACAAGA
ACTTGAAACC
ACTCAAAAAC
ATTTGCAAGA
AACTAAATTA
CAACTTGTTA
AAGAAGAATA
TATCACATCA
GCTTTGGAAA
GTACTGAGGA
GAAACTTCAT
GATGCTGCCA
GCAAGCTGCT
TAACACAGTT
GAAGAAACTA
CAAAAGATGT
ATCTGGTCTC
CATTCCAAAC
TGGATCGTAA
GAAGGCAGTT
GACCAACACA
ATGCAGAAGC
TCAGGATATT
TTTGGCAAAA
ACCTGAATAG
TCTGTTTAAT
AATATGGAAG
AATTAATTAA
GGATGGCAGC
TCAAAGCAAA
AGGCCATGCT
AGAAGTACAT
AAGACCTTAT
TTGGTAATCT
GCTGTCTTCC
AGTGTCTCTG
CATTAGATAC
CATTACTACA
GTAGCACTTG
GATCTCTCAC
ATCTATTCCA
GAAAATGTGT
CTACTCATGT
TTCTCAGATT
TTTAATATGA
TACTAAAAGA
ACAATCATTA
GCAGCAGAAA
GTAAAACTGT
ACTACAGGAA
TTGATTAATG
TACTCAAGAC
TGATCTTCTA
AGTTCACTGG
AAATGATTTT
ATCCCCAACT
GTGGTGTCTA
TACTGAAAAT
CAATAGTCAA
CTAAAGCATA
TTTTCAAGAC
TTCATTGACA
GTGGCCGATA
AGATAGAAGA
TCAAAAAAAG
GAACTAGATG
GCTTTCTCAG
TATACTGTGT
AACAATCTAC
ATGAACTACA
AGAAAATACC
ATTTGTTCCT
TGGTTGAGTC
ACAAAAGCAA
TGTGGAAACC
TAACTGAAGA
CCTGAAGACA
ATAAAGCAGA
CCCATTCCCA
GGAACTTTGC
AAGTTAATGA
ATCTTTGGAC
AGAGAGATTC
TGTGCTTTGG
AGGAAAAGTG
TGAAAATATA
CAGAAACCAC
TTAGTAGTGT
CCAGGAAAAT
ATACAGCAGA
AATCTAAGGA
TATAGTCAAC
AAAATGACTT
TTCACAGTCA
AAAATTTTGT
GCTGATTCTG
ATGGCTTCTC
ACAGGAACTC
AGAAATTTTA
ACCAAGAAGG
TACAAAATTG
GTTGAAGAAT
CTGTGAAACA
CTCTGATAAA
CTCAATGGCA
ACCTGGAAAA
AATATCTCAA
GAGACTGAAC
AGAGATGTGA
ATCTCTGAAC
ACAAGAACAG
TTTATTTTTC
TGAACAGTGG
GTATCTTCCT
TAAATGAAAG
GGAACAGGAA
CTTCACAACT
TATTGGAGGT
TGTAAGCCAA
TGTTGTGAGG
CTTCAAGTTC
AGACATCACT
GAGAAATCAG
ATGGACGTAA
GGCAGCTCAT
GAGAAACAGC
ATAACATTTT
TCTTGATCAG
ATGACTATTG
ATGAAGATAA
ATTGATAGCA
CAAAATCTAG
AACTTAATGA
AACCATAAAA
ATTGGTTTGA
CTAAGCTTAA
TTGCTTTCTG
GAACAGGATC
TGAAACTGGA
TATCCCAACA
GGTACGACAC
CACAGAGGAA
AAGTTATTTA
TACCCATCAA
CACTGGTAAG
AACTGAACCA
CGTGAACATC
TCCTTGATCA
GCTGAAAAGG
AAACAGCCTG
AGCTGTTAAT
GATGCTAAAC
TGTTCAGAAA
ACAACAAAGA
AGAGACAATT
CCGGATGTGG
ATGTAGAAGA
GGCAGTTCTG
GGGCAGTATA
CTGAAGAACC
TCTAAGTCAA
GAGCCATCTG
TAGATGCTGG
TGTGGATTGT
TCATCAATTG
GCGGGGTTCC
ATTTTTCCAG
CATAAAAAAT
CACATGGAAA
AGACAAAGAA
AACAGAGGCA
TTAACACACT
GGAGAGGTCT
AAAGTGGAAG
AAACTACAGA
GCACTTGGTT
ACAAAGAGCA
GATTACCTCT
GCGAGCCCAG
ATCAACCTTC
TCGAGTACCC
ATACGACGTA
CCTGATTATG
CAACTTATCC
TTATGATGTA
CCTGATTATG
CTAGCTATCC
TTATGATGTC
CCAGACTACG
CATAGGGATC
CGCGGCCGCA
AGGATCTGCG
ATCGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACGGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGCTGAAGC
TTCGAGGGGC
TCGCATCTCT
CCTTCACGCG
CCCGCCGCCC
TACCTGAGGC
CGCCATCCAC
GCCGGTTGAG
TCGCGTTCTG
CCGCCTCCCG
CCTGTGGTGC
CTCCTGAACT
GCGTCCGCCG
TCTAGGTAAG
TTTAAAGCTC
AGGTCGAGAC
CGGGCCTTTG
TCCGGCGCTC
CCTTGGAGCC
TACCTAGACT
CAGCCGGCTC
TCCACGCTTT
GCCTGACCCT
GCTTGCTCAA
CTCTACGTCT
TTGTTTCGTT
TTCTGTTCTG
CGCCGTTACA
GATCCAAGCT
GTGACCGGCG
CCTACGCTAG
ACGCCACCAT
GGAGAGCGAC
GAGAGCGGCC
TGATGGAAGA
CGCCAAAAAC
ATAAAGAAAG
GCCCGGCGCC
ATTCTATCCG
CTGGAAGATG
GAACCGCTGG
AGAGCAACTG
CATAAGGCTA
TGAAGAGATA
CGCCCTGGTT
CCTGGAACAA
TTGCTTTTAC
AGATGCACAT
ATCGAGGTGG
ACATCACTTA
CGCTGAGTAC
TTCGAAATGT
CCGTTCGGTT
GGCAGAAGCT
ATGAAACGAT
ATGGGCTGAA
TACAAATCAC
AGAATCGTCG
TATGCAGTGA
AAACTCTCTT
CAATTCTTTA
TGCCGGTGTT
GGGCGCGTTA
TTTATCGGAG
TTGCAGTTGC
GCCCGCGAAC
GACATTTATA
ATGAACGTGA
ATTGCTCAAC
AGTATGGGCA
TTTCGCAGCC
TACCGTGGTG
TTCGTTTCCA
AAAAGGGGTT
GCAAAAAATT
TTGAACGTGC
AAAAAAAGCT
CCCAATCATC
CAAAAAATTA
TTATCATGGA
TTCTAAAACG
GATTACCAGG
GATTTCAGTC
GATGTACACG
TTCGTCACAT
CTCATCTACC
TCCCGGTTTT
AATGAATACG
ATTTTGTGCC
AGAGTCCTTC
GATAGGGACA
AGACAATTGC
ACTGATCATG
AACTCCTCTG
GATCTACTGG
TCTGCCTAAA
GGTGTCGCTC
TGCCTCATAG
AACTGCCTGC
GTGAGATTCT
CGCATGCCAG
AGATCCTATT
TTTGGCAATC
AAATCATTCC
GGATACTGCG
ATTTTAAGTG
TTGTTCCATT
CCATCACGGT
TTTGGAATGT
TTACTACACT
CGGATATTTG
ATATGTGGAT
TTCGAGTCGT
CTTAATGTAT
AGATTTGAAG
AAGAGCTGTT
TCTGAGGAGC
CTTCAGGATT
ACAAGATTCA
AAGTGCGCTG
CTGGTGCCAA
CCCTATTCTC
CTTCTTCGCC
AAAAGCACTC
TGATTGACAA
ATACGATTTA
TCTAATTTAC
ACGAAATTGC
TTCTGGTGGC
GCTCCCCTCT
CTAAGGAAGT
CGGGGAAGCG
GTTGCCAAGA
GGTTCCATCT
GCCAGGTATC
AGGCAAGGAT
ATGGGCTCAC
TGAGACTACA
TCAGCTATTC
TGATTACACC
CGAGGGGGAT
GATAAACCGG
GCGCGGTCGG
TAAAGTTGTT
CCATTTTTTG
AAGCGAAGGT
TGTGGATCTG
GATACCGGGA
AAACGCTGGG
CGTTAATCAA
AGAGGCGAAC
TGTGTGTGAG
AGGTCCTATG
ATTATGTCCG
GTTATGTAAA
CAATCCGGAA
GCGACCAACG
CCTTGATTGA
CAAGGATGGA
TGGCTACATT
CTGGAGACAT
AGCTTACTGG
GACGAAGACG
AACACTTCTT
CATCGTTGAC
CGCCTGAAGT
CTCTGATTAA
GTACAAAGGC
TATCAGGTGG
CTCCCGCTGA
ATTGGAATCC
ATCTTGCTCC
AACACCCCAA
CATCTTCGAC
GCAGGTGTCG
CAGGTCTTCC
CGACGATGAC
GCCGGTGAAC
TTCCCGCCGC
CGTTGTTGTT
TTGGAGCACG
GAAAGACGAT
GACGGAAAAA
GAGATCGTGG
ATTACGTCGC
CAGTCAAGTA
ACAACCGCGA
AAAAGTTGCG
CGGAGGAGTT
GTGTTTGTGG
ACGAAGTACC
GAAAGGTCTT
ACCGGAAAAC
TCGACGCAAG
AAAAATCAGA
GAGATCCTCA
TAAAGGCCAA
GAAGGGCGGA
AAGATCGCCG
TGAGATCCCG
CGCTCAGTCG
TCCAATTCTG
CCGTGGACGG
CACCGCCGGA
CCCGGCTCCA
CCGGATCTCG
CGAGGGCAGA
GGAAGTCTTC
TAACATGCGG
TGACGTGGAG
GAGAATCCCG
GCCCTATGAC
CGAGTACAAG
CCCACGGTGC
GCCTCGCCAC
CCGCGACGAC
GTCCCCAGGG
CCGTACGCAC
CCTCGCCGCC
GCGTTCGCCG
ACTACCCCGC
CACGCGCCAC
ACCGTCGATC
CGGACCGCCA
CATCGAGCGG
GTCACCGAGC
TGCAAGAACT
CTTCCTCACG
CGCGTCGGGC
TCGACATCGG
CAAGGTGTGG
GTCGCGGACG
ACGGCGCCGC
GGTGGCGGTC
TGGACCACGC
CGGAGAGCGT
CGAAGCGGGG
GCGGTGTTCG
CCGAGATCGG
CCCGCGCATG
GCCGAGTTGA
GCGGTTCCCG
GCTGGCCGCG
CAGCAACAGA
TGGAAGGCCT
CCTGGCGCCG
CACCGGCCCA
AGGAGCCCGC
GTGGTTCCTG
GCCACCGTCG
GCGTCTCGCC
CGACCACCAG
GGCAAGGGTC
TGGGCAGCGC
CGTCGTGCTC
CCCGGAGTGG
AGGCGGCCGA
GCGCGCCGGG
GTGCCCGCCT
TCCTGGAGAC
CTCCGCGCCC
CGCAACCTCC
CCTTCTACGA
GCGGCTCGGC
TTCACCGTCA
CCGCCGACGT
CGAGGTGCCC
GAAGGACCGC
GCACCTGGTG
CATGACCCGC
AAGCCCGGTG
CCTGAAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
GCTGACGTCC
TTTCCATGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTCCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GCCGCCTCCC
CGCCTGGTAC
CTTTAAGACC
AATGACTTAC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAAATAAGA
TCTGCTTTTT
GCTTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTAGTAGTTC
ATGTCATCTT
ATTATTCAGT
ATTTATAACT
TGCAAAGAAA
TGAATATCAG
AGAGTGAGAG
GAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGGCTCTAGC
TATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
GGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
ACTTTTGCAG
AGACGGCCCA
AATTCGTAAT
CATGGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGGAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCTAAGAAAC
CATTATTATC
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTCTCGC
GCGTTTCGGT
GATGACGGTG
AAAACCTCTG
ACACATGCAG
CTCCCGGAGA
CGGTCACAGC
TTGTCTGTAA
GCGGATGCCG
GGAGCAGACA
AGCCCGTCAG
GGCGCGTCAG
CGGGTGTTGG
CGGGTGTCGG
GGCTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGCGCCATTC
GCCATTCAGG
CTGCGCAACT
GTTGGGAAGG
GCGATCGGTG
CGGGCCTCTT
CGCTATTACG
CCAGCTGGCG
AAAGGGGGAT
GTGCTGCAAG
GCGATTAAGT
TGGGTAACGC
CAGGGTTTTC
CCAGTCACGA
CGTTGTAAAA
CGACGGCCAG
TGCCAAGCTG
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