名称: | pLV3-CMV-Ascc3(mouse)-3×FLAG-CopGFP-Puro |
---|---|
别称: | NM_198007.2;ASC1p200; B630009I04Rik; D430001L07Rik;D630041L21; Helic1; RNAH |
基因长: | 6594bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-Ascc3(mouse)-3×FLAG-CopGFP-Puro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动小鼠源Ascc3基因转录,促进Ascc3基因C端融合表达3×FLAG标签,便于FLAG抗体对表达的融合蛋白进行检测、定位和纯化等。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带的CopGFP绿色荧光蛋白用于观察目的基因的表达和定位情况。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGG
CTCTACCTCG
GCTCACAGGA
GCCTTGCGCT
CCTTTTCAAA
TGTCACCAAG
CAAGATAATT
ACAATGAAGA
AGTAGCTGAC
TTGAAGTTGA
AGCGATCTAA
ACTTCATGAA
CAAGTTTTAG
ATTTTGGCCT
TACATGGAAG
AAGATAGTGA
AATTTTTGAA
TGAAAAGCTA
GAGAAGAATA
AAATGCAAAA
TATAAATGAA
GACTTAAAAG
ACATACTACA
AGCTGCAAAA
CAAATCGTTG
GAACGGATAA
TGGGAGAGAA
GCAATTGAGA
GTGGGGCTGC
ATTTCTCTTT
ATGACATTTC
ACATGACGGA
CTCTGTTGGT
TACATGGAGA
CAAAGGCTAT
CAGACAGACG
TTTGGTCCCT
TTCCATCGTC
ATCTGCTACT
TCAGCTTGTA
ATGCCACTAA
TCGAATTATT
TCTCATTTTA
GTCAAGATGA
TCTCACTGCT
TTTGTCCAGA
TGGCAGAAAA
CCCATGTAAC
GATAGAGTGG
TTTTTGGCAA
AAACTTAGCA
TTTTCTTTTG
ACATGTATGA
TCTGGACCAC
TTTGATGAAT
TACCAATAAA
TGGTGAATCC
CAGAAAACTA
TAAGCTTAGA
CTACAAGAAG
TTCCTGAATG
AACAGTTCCA
GGAACCCTAC
ACTCCTGAGC
TCAAGCCTGT
GGAGAAAACA
AATGGCTCCC
TATTGTGGTG
TGAAGTTGAG
AAGTACTTAA
ACGCAACGTT
AAAGGAAATG
ACTGAAGCAG
CAAGAGTTGA
AGATCTTTGC
TGTACTCTAT
ATGACATGCT
TGCTTCTGCT
AAAAGCGGCG
ATGAACTTCA
GGACGAGCTG
TTTGAATTGC
TGGGACCTGA
AGGACTGGAT
CTGATTGAGA
AGCTGCTCCA
GAACAGAATT
ACAATTGTAG
ATCGGTTCCT
TAATTCCTCG
AGCGATCATA
AGTTTCAAGT
TCTCCAAGAT
AGTTGTAAAA
AGATTTTAGG
AGAGAATTCA
AAGCCTAATT
ATGGATGTCA
AGTCACTATT
CAATCTGAAC
AAGAAAAACA
GTTAATGAAA
CAATATCGAC
GTGAAGAAAA
AAGAATTGCC
AGGCGAGAAA
AAAAGGCTGG
AGAAGATGGG
GAAGTTTCTG
GAGAAGGAGT
TTTGCCCTTT
GATCCTAAGG
AACTCCGGAT
ACAGAGAGAA
CATGCACTTC
TGAATGCTAG
AAATGCTCCA
ATTTTAGGAA
GACAGAGAGA
CGTGGAGTTT
GAAAAAATCC
GTTATCCTCA
TGTTTATGAT
TCCCAGGCTC
AGGCCCGAGA
AACATCAGCA
TTCATAGCTG
GAGCCAAGAT
GATTTTACCA
GAAGGAATCC
AGAGAGAGAA
TACCAAGCTG
TATGAGGAAG
TCAGGATCCC
CTATGGTGAA
CCAATGCCAG
TTGGGTTTGA
GGAAAAACCA
GTTTATATCA
AAGATTTAGA
TGAGGTTGGA
CAGTTGGCTT
TTAAAGGAAT
GAAGAGACTC
AATAGAATCC
AGTCTATAGT
ATTTGAAACA
GCCTACAACA
CCAACGAAAA
CATGCTGATC
TGTGCCCCTA
CGGGAGCTGG
GAAGACCAAT
ATTGCAATGC
TTACTATCTT
ACATGAAATC
CGCCAACACT
TTCATCAAGG
TGTTATTAAA
AAGAATGAGT
TTAAGATTGT
GTATGTCGCT
CCAATGAAAG
CCTTGGCAGC
TGAGATGACA
AATTACTTCA
GCAAACGTCT
AGAGCCACTG
GGCATTGTTG
TGAAAGAATT
GACTGGAGAT
ATGCAGTTGT
CAAAAAGTGA
AATTTTACGA
ACTCAGATGC
TGGTGACCAC
ACCAGAAAAA
TGGGATGTAG
TAACAAGAAA
GAGTGTTGGG
GATGTTGCTC
TTTCCCAAAT
CGTAAAACTC
CTGATTCTTG
ACGAGGTGCA
CTTGCTGCAT
GAAGACAGAG
GCCCAGTGCT
AGAAAGCATA
GTCGCCAGGA
CCTTACGGCA
GGTTGAATCC
ACACAGAGTA
TGATAAGAAT
TCTTGGATTG
TCTGCAACCT
TACCCAACTA
CCTCGATGTT
GCTACATTTT
TGCATGTTAA
CCCCTACATT
GGACTTTTCT
ACTTTGATGG
CCGTTTTCGA
CCTGTACCTC
TTGGACAGAC
ATTTTTAGGG
ATTAAAAGTA
CAAACAAGAT
GCAGCAGTTA
AATAACATGG
ATGAAGTGTG
CTACGAAAGT
GTTCTGAAAC
AAGTAAAGGC
TGGACACCAG
GTGATGGTAT
TTGTACATGC
TCGCAATGCC
ACTGTAAGGA
CCGCTATGTC
TCTAATAGAG
AGAGCAAAAA
ATAGTGGCCA
AATTTCTTGC
TTTCTACCCA
CCGAAGGGCC
AGAGTACGGA
CATGCATTAA
AACAGGTACA
AAAGTCAAGA
AATAAGCAAG
TGCGAGAATT
ATTCTCCGAT
GGCTTTAGCA
TTCATCATGC
CGGGATGCTT
CGGCAAGACA
GAAATTTGGT
TGAAAATTTG
TTTTCAAATG
GGCATATCAA
AGTCCTAGTC
TGTACAGCCA
CATTAGCCTG
GGGTGTCAAT
CTTCCTGCAC
ATGCCGTGGT
TATTAAGGGA
ACACAAATAT
ACGCTGCCAA
AAGAGGCTCC
TTTGTTGACC
TTGGAATTTT
AGATGTCATG
CAGATATTTG
GCCGAGCTGG
ACGACCGCAA
TTTGACAAAT
TTGGGGAAGG
AATCATTATA
ACAACCCACG
ATAAACTCAG
CCATTACCTC
AGTTTGCTCA
CTCAACAAAA
CCCAATTGAG
AGTCAGTTTC
TGGAAAGTCT
TGCAGATAAC
CTAAATGCAG
AGATTGCGCT
GGGGACAGTC
ACCAACGTGG
AAGAGGCAGT
GAGGTGGATG
AGTTACACTT
ACCTCTATGT
GCGCATGCGA
GCAAACCCGC
TAGCATACGG
CATCAGTCAC
AAAGCGTACC
AGATTGACCC
AACACTAAGG
AAGCATCGAG
AACAGCTCCT
TATTGAAGTT
GGACAAAAGT
TGGATAAAGC
AAAGATGATT
CGTTTTGAAG
AACGAACTGG
CTATTTTTCT
TCTACTGATT
TGGGTAGAAC
TGCCAGCCAC
TTCTATATTA
AATACAACAC
CATTGAGACA
TTTAATGAAC
TCTTTGATGC
ACACAAAACA
GAGGGTGATA
TCTTTGCTAT
TGTTTCCAAA
GCTGAAGAAT
TTGATCAAAT
TAAGGTCAGA
GAAGAAGAAA
TAGAAGAATT
AGATGCCTTG
TTAAACAATT
TCTGTGAACT
CTCAGCTCCA
GGAGGTGTGG
AAAATAGTTA
TGGGAAAATA
AATATCCTAC
TTCAAACCTA
CATCAGCCGA
GGAGAGATGG
ACAGTTTCTC
CCTCATATCA
GATTCTGCAT
ATGTCGCCCA
GAATGCAGCT
AGAATTGTCC
GTGCTCTCTT
TGAAATTGCA
CTGAGGAAAC
GTTGGCCTAC
CATGACCTAC
AGGCTTTTGA
ATCTTAGTAA
AGTCATTGAC
AAGAGACTCT
GGGGTTGGGC
TAGCCCTTTG
AGACAATTTT
CTGTGTTACC
ACCACACATT
CTAACAAGAT
TAGAAGAAAA
AAACCTTACT
GTGGATAAAC
TCAAAGACAT
GAGAAAAGAT
GAAATAGGTC
ACATTTTGCA
TCATGTGAAT
ATTGGACTGA
AGGTCAAACA
GTGTGTTCAT
CAAATTCCTT
CTGTTACAAT
GGAAGCTTCC
ATTCAGCCCA
TCACACGGAC
TGTCCTCCGA
GTATCACTGA
ACATCCACCC
TGATTTCTCT
TGGAATGACC
AGGTTCATGG
AACAGTGGGG
GAACCATGGT
GGATTTGGGT
AGAAGATCCT
ACAAATGACC
ATATTTATCA
CTCGGAGTAC
TTTCTAGCTC
TTAAAAAACA
GGTTATTAAT
AAGGAAGCTC
AACTTCTGGT
ATTTACAATT
CCTATTTTTG
AGCCTTTGCC
CTCTCAGTAC
TACATTCGAG
CAGTGTCTGA
TCGATGGCTA
GGAGCTGAAG
CTGTTTGCAT
TATCAACTTT
CAACATCTGA
TTCTACCAGA
GAGACATCCT
CCCCATACAG
AATTATTGGA
CCTTCAACCT
TTACCAATTA
CAGCTTTGGG
GTGCAAAGCA
TATGAAGCTC
TATATAACTT
CAGCCACTTT
AACCCTGTAC
AGACGCAGAT
ATTCCACACA
CTGTATCACA
CAGACTGTAA
TGTTCTGCTT
GGCGCCCCCA
CCGGATCAGG
AAAGACAGTT
GCAGCTGAAC
TAGCCATTTT
CAGAGTTTTC
AACAAATATC
CTACTTCAAA
GGCAGTATAT
ATTGCACCCC
TCAAAGCTCT
AGTACGTGAA
AGAATGGATG
ATTGGAAAAT
TAGGATAGAA
GAAAAACTTG
GTAAAAAAGT
TATTGAACTA
ACTGGGGATG
TGACTCCAGA
CATGAAATCC
ATTGCTAAGG
CTGACCTTAT
TGTCACCACA
CCAGAAAAGT
GGGATGGAGT
TAGTCGAAGC
TGGCAGAATA
GAAGCTATGT
TCAGCAGGTC
AACATTCTCA
TCATAGATGA
GATCCATCTG
CTAGGGGAGG
AGAGAGGCCC
AGTTTTAGAA
GTCATTGTAT
CTCGAACAAA
CTTTATCTCA
TCACACACAG
AAAAACCAGT
TAGAATAGTT
GGACTGTCCA
CTGCATTAGC
TAATGCTAGA
GATCTTGCTG
ACTGGCTCAA
CATTAAGCAG
ATGGGTTTGT
TTAACTTCCG
ACCATCAGTT
CGCCCAGTTC
CACTGGAAGT
TCACATTCAG
GGTTTCCCAG
GACAACATTA
CTGCCCACGC
ATGGCTAGTA
TGAACAAGCC
TGCATTTCAG
GCAATTAGAA
GTCACTCTCC
AGCCAAGCCT
GTTTTGATAT
TTGTCTCATC
AAGACGTCAA
ACACGACTTA
CAGCCTTAGA
GTTGATAGCA
TTTCTGGCCA
CTGAAGAAGA
CCCAAAGCAG
TGGTTGAATA
TGGATGAGCA
AGAGATGGAC
AACATCATTG
GAACAGTACG
AGATTCCAAC
CTCAAGCTGA
CTCTAGCTTT
TGGAATAGGA
ATGCATCATG
CTGGCCTGCA
TGAAAGAGAC
CGAAAAACTG
TAGAAGAGCT
GTTTGTGAAC
TGTAAAGTTC
AGGTTCTCAT
TGCTACAAGC
ACATTAGCAT
GGGGTGTAAA
CTTTCCAGCA
CACTTAGTAA
TTATTAAGGG
AACTGAATAT
TATGATGGAA
AGACAAGACG
TTATGTGGAT
TTTCCCATCA
CAGATGTGCT
GCAGATGATG
GGACGAGCTG
GGAGACCTCA
GTTTGATGAC
CAGGGCAAAG
CTGTAATTCT
AGTTCATGAT
ATAAAAAAAG
ATTTTTATAA
AAAGTTTCTT
TATGAACCTT
TCCCAGTAGA
ATCAAGCTTA
TTAGGAGTGT
TGTCTGACCA
CTTAAATGCA
GAGATTGCTG
GAGGTACAAT
AACATCAAAG
CAAGATGCAA
TGGATTATAT
CACCTGGACT
TACTTTTTCC
GGCGTCTTAT
CATGAATCCC
AGCTACTACA
GCTTGGGTGA
CGTCAGCCAG
GACTCCATAA
ACAAGTTCCT
GTCACATCTG
ATTGGGCAGT
CTCTGGTTGA
ATTGGAGCTT
TCCCACTGCA
TTGAAGTTGG
AGAGGATAAT
CGCACCATTG
AACCACTGAC
ATGTGGTCGA
ATTGCCTCCT
ATTACTATTT
GAAGCACAAA
ACTGTCAAAA
TGTTCAAGGA
CCGTTTGAAA
CCTGAGTGCA
GCACTGAGGA
ACTGCTGTCA
ATTCTGAGTG
ATGCAGAAGA
ATACACAGAC
TTACCAGTAA
GACACAATGA
GGACCATACG
AATAATGAAT
TGGCCAAATG
TCTTCCCATT
GAATTAAATC
CTCATTCATT
TGACAGCCCT
CACACCAAAG
CCCATCTCTT
GCTTCAAGCG
CATCTCAGCC
GAGCCATGCT
GCCCTGCCCA
GATTATGATA
CTGATACCAA
AACAGTCTTA
GACCAAGCTC
TCAGAGTATG
CCAGGCAATG
CTGGATGTGG
CTGCAAGCCA
GGGATGGCTA
GTGACCGTCC
TGAATATCAC
CCACCTGATT
CAGATGGTGA
TCCAAGGCCG
ATGGCTCAAG
GACTCTTCTC
TTCTCACAAT
ACCAAACATA
GAGCAGCACC
ACCTTCACCT
TTTCAGGAAA
TGGAAGCCAC
CTGTAAAGAG
CTCACATGCT
AAGTGCCGAA
CATCCATTGA
GTGCCTTCCT
GAGCTGATCC
ATGCCTGTGA
AGGGAAGGAC
CATGTGTTCA
GCTCCATGGT
AGAGAAAGAG
TTACAGCCTG
CAAAAACAAA
GCAGGCATGG
AATTTCTTAT
CTCGCTTGCC
AGTGATAAAT
GTTGGCATAA
GTGTTAAAGG
CTCATGGGAT
GACTTAGTTG
AAGGACATAA
TGAACTCTCC
ATCTCAACTC
TGACAGCAGA
CAAACGGGAT
GAAAACAAAT
GGATCAAGTT
GCATGCTGAT
CAGGAGTATG
TGCTTCAAGT
CAGCCTACAG
AGAGTCCATT
TTGGGCTTCC
AAAGGGAAAA
CATGAGAATC
ACGCAGTTAC
TCCTCGATTT
CCAAAATTAA
AAGATGAAGG
CTGGTTTTTG
ATATTAGGAG
AAGTGGATAA
GAGAGAGCTG
ATGGCTGTAA
AACGAGTAGG
ATTTGTTCGA
ACACATCATG
ATGCTTCCAT
CTCGTTCTTT
ACTCCTGAAA
CACCTGGAAG
ATATATCTTT
ACCCTGTATC
TCATGAGTGA
CTGTTACCTC
GGCCTAGACC
AGCAGTATGA
CATTTATCTC
AATGTCATAA
AAGCAAACAT
TTCTACAAAG
GACTCTGATG
TCTTCACTGA
CTTGTCAGTA
GGATCCGACT
ACAAAGACCA
TGACGGTGAT
TATAAAGATC
ATGACATCGA
CTACAAGGAT
GACGATGACA
AGTAGCGGCC
GCGAAGGATC
TGCGATCGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACGGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGCTG
AAGCTTCGAG
GGGCTCGCAT
CTCTCCTTCA
CGCGCCCGCC
GCCCTACCTG
AGGCCGCCAT
CCACGCCGGT
TGAGTCGCGT
TCTGCCGCCT
CCCGCCTGTG
GTGCCTCCTG
AACTGCGTCC
GCCGTCTAGG
TAAGTTTAAA
GCTCAGGTCG
AGACCGGGCC
TTTGTCCGGC
GCTCCCTTGG
AGCCTACCTA
GACTCAGCCG
GCTCTCCACG
CTTTGCCTGA
CCCTGCTTGC
TCAACTCTAC
GTCTTTGTTT
CGTTTTCTGT
TCTGCGCCGT
TACAGATCCA
AGCTGTGACC
GGCGCCTACG
CTAGACGCCA
CCATGGAGAG
CGACGAGAGC
GGCCTGCCCG
CCATGGAGAT
CGAGTGCCGC
ATCACCGGCA
CCCTGAACGG
CGTGGAGTTC
GAGCTGGTGG
GCGGCGGAGA
GGGCACCCCC
AAGCAGGGCC
GCATGACCAA
CAAGATGAAG
AGCACCAAAG
GCGCCCTGAC
CTTCAGCCCC
TACCTGCTGA
GCCACGTGAT
GGGCTACGGC
TTCTACCACT
TCGGCACCTA
CCCCAGCGGC
TACGAGAACC
CCTTCCTGCA
CGCCATCAAC
AACGGCGGCT
ACACCAACAC
CCGCATCGAG
AAGTACGAGG
ACGGCGGCGT
GCTGCACGTG
AGCTTCAGCT
ACCGCTACGA
GGCCGGCCGC
GTGATCGGCG
ACTTCAAGGT
GGTGGGCACC
GGCTTCCCCG
AGGACAGCGT
GATCTTCACC
GACAAGATCA
TCCGCAGCAA
CGCCACCGTG
GAGCACCTGC
ACCCCATGGG
CGATAACGTG
CTGGTGGGCA
GCTTCGCCCG
CACCTTCAGC
CTGCGCGACG
GCGGCTACTA
CAGCTTCGTG
GTGGACAGCC
ACATGCACTT
CAAGAGCGCC
ATCCACCCCA
GCATCCTGCA
GAACGGGGGC
CCCATGTTCG
CCTTCCGCCG
CGTGGAGGAG
CTGCACAGCA
ACACCGAGCT
GGGCATCGTG
GAGTACCAGC
ACGCCTTCAA
GACCCCCATC
GCCTTCGCCA
GATCCCGCGC
TCAGTCGTCC
AATTCTGCCG
TGGACGGCAC
CGCCGGACCC
GGCTCCACCG
GATCTCGCGA
GGGCAGAGGA
AGTCTTCTAA
CATGCGGTGA
CGTGGAGGAG
AATCCCGGCC
CTATGACCGA
GTACAAGCCC
ACGGTGCGCC
TCGCCACCCG
CGACGACGTC
CCCAGGGCCG
TACGCACCCT
CGCCGCCGCG
TTCGCCGACT
ACCCCGCCAC
GCGCCACACC
GTCGATCCGG
ACCGCCACAT
CGAGCGGGTC
ACCGAGCTGC
AAGAACTCTT
CCTCACGCGC
GTCGGGCTCG
ACATCGGCAA
GGTGTGGGTC
GCGGACGACG
GCGCCGCGGT
GGCGGTCTGG
ACCACGCCGG
AGAGCGTCGA
AGCGGGGGCG
GTGTTCGCCG
AGATCGGCCC
GCGCATGGCC
GAGTTGAGCG
GTTCCCGGCT
GGCCGCGCAG
CAACAGATGG
AAGGCCTCCT
GGCGCCGCAC
CGGCCCAAGG
AGCCCGCGTG
GTTCCTGGCC
ACCGTCGGCG
TCTCGCCCGA
CCACCAGGGC
AAGGGTCTGG
GCAGCGCCGT
CGTGCTCCCC
GGAGTGGAGG
CGGCCGAGCG
CGCCGGGGTG
CCCGCCTTCC
TGGAGACCTC
CGCGCCCCGC
AACCTCCCCT
TCTACGAGCG
GCTCGGCTTC
ACCGTCACCG
CCGACGTCGA
GGTGCCCGAA
GGACCGCGCA
CCTGGTGCAT
GACCCGCAAG
CCCGGTGCCT
GAAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAATC
ATCGTCCTTT
CCTTGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CCTGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGGGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AGATAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
AGTAGTTCAT
GTCATCTTAT
TATTCAGTAT
TTATAACTTG
CAAAGAAATG
AATATCAGAG
AGTGAGAGGA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GCTCTAGCTA
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCG
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGA
CTTTTGCAGA
GACCAAATTC
GTAATCATGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GGACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTCTAAG
AAACCATTAT
TATCATGACA
TTAACCTATA
AAAATAGGCG
TATCACGAGG
CCCTTTCGTC
TCGCGCGTTT
CGGTGATGAC
GGTGAAAACC
TCTGACACAT
GCAGCTCCCG
GAGACGGTCA
CAGCTTGTCT
GTAAGCGGAT
GCCGGGAGCA
GACAAGCCCG
TCAGGGCGCG
TCAGCGGGTG
TTGGCGGGTG
TCGGGGCTGG
CTTAACTATG
CGGCATCAGA
GCAGATTGTA
CTGAGAGTGC
ACCATATGCG
GTGTGAAATA
CCGCACAGAT
GCGTAAGGAG
AAAATACCGC
ATCAGGCGCC
ATTCGCCATT
CAGGCTGCGC
AACTGTTGGG
AAGGGCGATC
GGTGCGGGCC
TCTTCGCTAT
TACGCCAGCT
GGCGAAAGGG
GGATGTGCTG
CAAGGCGATT
AAGTTGGGTA
ACGCCAGGGT
TTTCCCAGTC
ACGACGTTGT
AAAACGACGG
CCAGTGCCAA
GCTG