名称: | pEF1a-PEmax-P2A-GFP |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 化脓性链球菌 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pCMV-PEmax-P2A-GFP |
正向引物: | |
反向引物: | AmpNR |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCAAAGAAGA
AGCGGAAAGT
CGACAAGAAG
TACAGCATCG
GCCTGGACAT
CGGCACCAAC
TCTGTGGGCT
GGGCCGTGAT
CACCGACGAG
TACAAGGTGC
CCAGCAAGAA
ATTCAAGGTG
CTGGGCAACA
CCGACCGGCA
CAGCATCAAG
AAGAACCTGA
TCGGAGCCCT
GCTGTTCGAC
AGCGGCGAAA
CAGCCGAGGC
CACCCGGCTG
AAGAGAACCG
CCAGAAGAAG
ATACACCAGA
CGGAAGAACC
GGATCTGCTA
TCTGCAAGAG
ATCTTCAGCA
ACGAGATGGC
CAAGGTGGAC
GACAGCTTCT
TCCACAGACT
GGAAGAGTCC
TTCCTGGTGG
AAGAGGATAA
GAAGCACGAG
CGGCACCCCA
TCTTCGGCAA
CATCGTGGAC
GAGGTGGCCT
ACCACGAGAA
GTACCCCACC
ATCTACCACC
TGAGAAAGAA
ACTGGTGGAC
AGCACCGACA
AGGCCGACCT
GCGGCTGATC
TATCTGGCCC
TGGCCCACAT
GATCAAGTTC
CGGGGCCACT
TCCTGATCGA
GGGCGACCTG
AACCCCGACA
ACAGCGACGT
GGACAAGCTG
TTCATCCAGC
TGGTGCAGAC
CTACAACCAG
CTGTTCGAGG
AAAACCCCAT
CAACGCCAGC
GGCGTGGACG
CCAAGGCCAT
CCTGTCTGCC
AGACTGAGCA
AGAGCAGAAA
GCTGGAAAAT
CTGATCGCCC
AGCTGCCCGG
CGAGAAGAAG
AATGGCCTGT
TCGGAAACCT
GATTGCCCTG
AGCCTGGGCC
TGACCCCCAA
CTTCAAGAGC
AACTTCGACC
TGGCCGAGGA
TGCCAAACTG
CAGCTGAGCA
AGGACACCTA
CGACGACGAC
CTGGACAACC
TGCTGGCCCA
GATCGGCGAC
CAGTACGCCG
ACCTGTTTCT
GGCCGCCAAG
AACCTGTCCG
ACGCCATCCT
GCTGAGCGAC
ATCCTGAGAG
TGAACACCGA
GATCACCAAG
GCCCCCCTGA
GCGCCTCTAT
GATCAAGAGA
TACGACGAGC
ACCACCAGGA
CCTGACCCTG
CTGAAAGCTC
TCGTGCGGCA
GCAGCTGCCT
GAGAAGTACA
AAGAGATTTT
CTTCGACCAG
AGCAAGAACG
GCTACGCCGG
CTACATTGAC
GGCGGAGCCA
GCCAGGAAGA
GTTCTACAAG
TTCATCAAGC
CCATCCTGGA
AAAGATGGAC
GGCACCGAGG
AACTGCTCGT
GAAGCTGAAG
AGAGAGGACC
TGCTGCGGAA
GCAGCGGACC
TTCGACAACG
GCAGCATCCC
CCACCAGATC
CACCTGGGAG
AGCTGCACGC
CATTCTGCGG
CGGCAGGAAG
ATTTTTACCC
ATTCCTGAAG
GACAACCGGG
AAAAGATCGA
GAAGATCCTG
ACCTTCCGCA
TCCCCTACTA
CGTGGGCCCT
CTGGCCAGGG
GAAACAGCAG
ATTCGCCTGG
ATGACCAGAA
AGAGCGAGGA
AACCATCACC
CCCTGGAACT
TCGAGGAAGT
GGTGGACAAG
GGCGCTTCCG
CCCAGAGCTT
CATCGAGCGG
ATGACCAACT
TCGATAAGAA
CCTGCCCAAC
GAGAAGGTGC
TGCCCAAGCA
CAGCCTGCTG
TACGAGTACT
TCACCGTGTA
TAACGAGCTG
ACCAAAGTGA
AATACGTGAC
CGAGGGAATG
AGAAAGCCCG
CCTTCCTGAG
CGGCGAGCAG
AAAAAGGCCA
TCGTGGACCT
GCTGTTCAAG
ACCAACCGGA
AAGTGACCGT
GAAGCAGCTG
AAAGAGGACT
ACTTCAAGAA
AATCGAGTGC
TTCGACTCCG
TGGAAATCTC
CGGCGTGGAA
GATCGGTTCA
ACGCCTCCCT
GGGCACATAC
CACGATCTGC
TGAAAATTAT
CAAGGACAAG
GACTTCCTGG
ACAATGAGGA
AAACGAGGAC
ATTCTGGAAG
ATATCGTGCT
GACCCTGACA
CTGTTTGAGG
ACAGAGAGAT
GATCGAGGAA
CGGCTGAAAA
CCTATGCCCA
CCTGTTCGAC
GACAAAGTGA
TGAAGCAGCT
GAAGCGGCGG
AGATACACCG
GCTGGGGCAG
GCTGAGCCGG
AAGCTGATCA
ACGGCATCCG
GGACAAGCAG
TCCGGCAAGA
CAATCCTGGA
TTTCCTGAAG
TCCGACGGCT
TCGCCAACAG
AAACTTCATG
CAGCTGATCC
ACGACGACAG
CCTGACCTTT
AAAGAGGACA
TCCAGAAAGC
CCAGGTGTCC
GGCCAGGGCG
ATAGCCTGCA
CGAGCACATT
GCCAATCTGG
CCGGCAGCCC
CGCCATTAAG
AAGGGCATCC
TGCAGACAGT
GAAGGTGGTG
GACGAGCTCG
TGAAAGTGAT
GGGCCGGCAC
AAGCCCGAGA
ACATCGTGAT
CGAAATGGCC
AGAGAGAACC
AGACCACCCA
GAAGGGACAG
AAGAACAGCC
GCGAGAGAAT
GAAGCGGATC
GAAGAGGGCA
TCAAAGAGCT
GGGCAGCCAG
ATCCTGAAAG
AACACCCCGT
GGAAAACACC
CAGCTGCAGA
ACGAGAAGCT
GTACCTGTAC
TACCTGCAGA
ATGGGCGGGA
TATGTACGTG
GACCAGGAAC
TGGACATCAA
CCGGCTGTCC
GACTACGATG
TGGACGCTAT
CGTGCCTCAG
AGCTTTCTGA
AGGACGACTC
CATCGACAAC
AAGGTGCTGA
CCAGAAGCGA
CAAGAACCGG
GGCAAGAGCG
ACAACGTGCC
CTCCGAAGAG
GTCGTGAAGA
AGATGAAGAA
CTACTGGCGG
CAGCTGCTGA
ACGCCAAGCT
GATTACCCAG
AGAAAGTTCG
ACAATCTGAC
CAAGGCCGAG
AGAGGCGGCC
TGAGCGAACT
GGATAAGGCC
GGCTTCATCA
AGAGACAGCT
GGTGGAAACC
CGGCAGATCA
CAAAGCACGT
GGCACAGATC
CTGGACTCCC
GGATGAACAC
TAAGTACGAC
GAGAATGACA
AGCTGATCCG
GGAAGTGAAA
GTGATCACCC
TGAAGTCCAA
GCTGGTGTCC
GATTTCCGGA
AGGATTTCCA
GTTTTACAAA
GTGCGCGAGA
TCAACAACTA
CCACCACGCC
CACGACGCCT
ACCTGAACGC
CGTCGTGGGA
ACCGCCCTGA
TCAAAAAGTA
CCCTAAGCTG
GAAAGCGAGT
TCGTGTACGG
CGACTACAAG
GTGTACGACG
TGCGGAAGAT
GATCGCCAAG
AGCGAGCAGG
AAATCGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACAGCAA
CATCATGAAC
TTTTTCAAGA
CCGAGATTAC
CCTGGCCAAC
GGCGAGATCC
GGAAGCGGCC
TCTGATCGAG
ACAAACGGCG
AAACCGGGGA
GATCGTGTGG
GATAAGGGCC
GGGATTTTGC
CACCGTGCGG
AAAGTGCTGA
GCATGCCCCA
AGTGAATATC
GTGAAAAAGA
CCGAGGTGCA
GACAGGCGGC
TTCAGCAAAG
AGTCTATCCT
GCCCAAGAGG
AACAGCGATA
AGCTGATCGC
CAGAAAGAAG
GACTGGGACC
CTAAGAAGTA
CGGCGGCTTC
GACAGCCCCA
CCGTGGCCTA
TTCTGTGCTG
GTGGTGGCCA
AAGTGGAAAA
GGGCAAGTCC
AAGAAACTGA
AGAGTGTGAA
AGAGCTGCTG
GGGATCACCA
TCATGGAAAG
AAGCAGCTTC
GAGAAGAATC
CCATCGACTT
TCTGGAAGCC
AAGGGCTACA
AAGAAGTGAA
AAAGGACCTG
ATCATCAAGC
TGCCTAAGTA
CTCCCTGTTC
GAGCTGGAAA
ACGGCCGGAA
GAGAATGCTG
GCCTCTGCCG
GCGAACTGCA
GAAGGGAAAC
GAACTGGCCC
TGCCCTCCAA
ATATGTGAAC
TTCCTGTACC
TGGCCAGCCA
CTATGAGAAG
CTGAAGGGCT
CCCCCGAGGA
TAATGAGCAG
AAACAGCTGT
TTGTGGAACA
GCACAAGCAC
TACCTGGACG
AGATCATCGA
GCAGATCAGC
GAGTTCTCCA
AGAGAGTGAT
CCTGGCCGAC
GCTAATCTGG
ACAAAGTGCT
GTCCGCCTAC
AACAAGCACC
GGGATAAGCC
CATCAGAGAG
CAGGCCGAGA
ATATCATCCA
CCTGTTTACC
CTGACCAATC
TGGGAGCCCC
TGCCGCCTTC
AAGTACTTTG
ACACCACCAT
CGACCGGAAG
AGGTACACCA
GCACCAAAGA
GGTGCTGGAC
GCCACCCTGA
TCCACCAGAG
CATCACCGGC
CTGTACGAGA
CACGGATCGA
CCTGTCTCAG
CTGGGAGGTG
ACTCCGGCGG
AAGCTCTGGT
GGCAGCAAGC
GGACCGCCGA
CGGCTCTGAA
TTCGAGAGCC
CTAAGAAGAA
AAGAAAGGTG
AGCGGAGGCT
CTAGCGGCGG
AAGCACCCTG
AACATTGAAG
ACGAGTATAG
ACTGCATGAA
ACAAGCAAGG
AACCCGACGT
GTCCCTGGGC
TCCACCTGGC
TGTCCGACTT
TCCCCAGGCC
TGGGCCGAGA
CAGGAGGAAT
GGGCCTGGCC
GTGCGGCAGG
CACCCCTGAT
CATCCCTCTG
AAGGCCACCT
CTACACCCGT
GAGCATCAAG
CAGTACCCTA
TGTCTCAGGA
GGCCAGACTG
GGCATCAAGC
CTCACATCCA
GAGGCTGCTG
GACCAGGGCA
TCCTGGTGCC
ATGCCAGAGC
CCCTGGAACA
CACCACTGCT
GCCCGTGAAG
AAGCCAGGCA
CCAATGACTA
TAGACCCGTG
CAGGATCTGA
GAGAGGTGAA
CAAGAGGGTG
GAGGATATCC
ACCCCACCGT
GCCCAACCCT
TACAATCTGC
TGTCCGGCCT
GCCCCCTTCT
CACCAGTGGT
ATACAGTGCT
GGACCTGAAG
GATGCCTTCT
TTTGTCTGAG
ACTGCACCCT
ACCAGCCAGC
CACTGTTCGC
CTTTGAGTGG
AGGGACCCTG
AGATGGGCAT
CTCTGGCCAG
CTGACCTGGA
CACGCCTGCC
TCAGGGCTTC
AAGAATAGCC
CAACACTGTT
TAACGAGGCC
CTGCACCGCG
ACCTGGCAGA
TTTCCGGATC
CAGCACCCAG
ATCTGATCCT
GCTGCAGTAC
GTGGACGATC
TGCTGCTGGC
CGCCACCAGC
GAGCTGGATT
GCCAGCAGGG
AACACGCGCC
CTGCTGCAGA
CCCTGGGAAA
CCTGGGATAT
AGGGCATCCG
CCAAGAAGGC
CCAGATCTGT
CAGAAGCAGG
TGAAGTACCT
GGGCTATCTG
CTGAAGGAGG
GCCAGAGATG
GCTGACAGAG
GCCAGGAAGG
AGACAGTGAT
GGGCCAGCCA
ACACCCAAGA
CCCCAAGACA
GCTGAGGGAG
TTCCTGGGCA
AAGCAGGATT
TTGCAGGCTG
TTCATCCCAG
GATTCGCAGA
GATGGCAGCA
CCTCTGTACC
CACTGACCAA
GCCGGGCACC
CTGTTTAATT
GGGGCCCTGA
CCAGCAGAAG
GCCTATCAGG
AGATCAAGCA
GGCCCTGCTG
ACAGCACCAG
CCCTGGGCCT
GCCAGACCTG
ACCAAGCCTT
TCGAGCTGTT
TGTGGATGAG
AAGCAGGGCT
ACGCCAAGGG
CGTGCTGACC
CAGAAGCTGG
GACCATGGAG
ACGGCCCGTG
GCCTATCTGT
CCAAGAAGCT
GGACCCAGTG
GCAGCAGGAT
GGCCACCATG
CCTGAGGATG
GTGGCAGCAA
TCGCCGTGCT
GACAAAGGAT
GCCGGCAAGC
TGACCATGGG
ACAGCCACTG
GTCATCCTGG
CACCACACGC
AGTGGAGGCC
CTGGTGAAGC
AGCCTCCAGA
TCGCTGGCTG
TCTAACGCCC
GGATGACACA
CTACCAGGCC
CTGCTGCTGG
ACACCGATCG
CGTGCAGTTT
GGCCCTGTGG
TGGCCCTGAA
TCCAGCCACC
CTGCTGCCTC
TGCCAGAGGA
GGGCCTGCAG
CACAACTGTC
TGGACATCCT
GGCAGAGGCA
CACGGAACAA
GGCCAGACCT
GACCGATCAG
CCCCTGCCTG
ACGCCGATCA
CACATGGTAT
ACCGATGGAA
GCTCCCTGCT
GCAGGAGGGC
CAGAGGAAGG
CAGGAGCAGC
AGTGACCACA
GAGACAGAAG
TGATCTGGGC
CAAGGCCCTG
CCAGCAGGCA
CATCCGCCCA
GCGGGCCGAG
CTGATCGCCC
TGACCCAGGC
CCTGAAGATG
GCCGAGGGCA
AGAAGCTGAA
CGTGTACACA
GACTCCAGAT
ATGCCTTCGC
CACCGCACAC
ATCCACGGAG
AGATCTACAG
GCGCCGGGGC
TGGCTGACCT
CTGAGGGCAA
GGAGATCAAG
AACAAGGATG
AGATCCTGGC
CCTGCTGAAG
GCCCTGTTTC
TGCCCAAGCG
GCTGAGCATC
ATCCACTGTC
CTGGACACCA
GAAGGGACAC
TCCGCCGAGG
CAAGGGGCAA
TCGGATGGCC
GACCAGGCCG
CCAGAAAGGC
TGCTATTACT
GAAACTCCCG
ACACTTCCAC
TCTGCTGATT
GAAAACTCCT
CCCCTTCTGG
CGGCTCAAAA
AGAACCGCCG
ACGGCAGCGA
ATTCGAGTCT
CCCAAGAAGA
AGAGGAAAGT
CGGCTCTGGC
CCTGCCGCTA
AGAGAGTGAA
GCTGGACGGA
TCCGGCGCAA
CAAACTTCTC
TCTGCTGAAA
CAAGCCGGAG
ATGTCGAAGA
GAATCCTGGA
CCGATGGTGA
GCAAGGGCGA
GGAGCTGTTC
ACCGGGGTGG
TGCCCATCCT
GGTCGAGCTG
GACGGCGACG
TAAACGGCCA
CAAGTTCAGC
GTGTCCGGCG
AGGGCGAGGG
CGATGCCACC
TACGGCAAGC
TGACCCTGAA
GTTCATCTGC
ACCACCGGCA
AGCTGCCCGT
GCCCTGGCCC
ACCCTCGTGA
CCACCCTGAC
CTATGGAGTG
CAGTGCTTCA
GCCGCTACCC
CGACCACATG
AAGCAGCACG
ACTTCTTCAA
GTCCGCCATG
CCCGAAGGCT
ACGTCCAGGA
GCGCACCATC
TTCTTCAAGG
ACGACGGCAA
CTACAAGACC
CGCGCCGAGG
TGAAGTTCGA
GGGCGACACC
CTGGTGAACC
GCATCGAGCT
GAAGGGCATC
GACTTCAAGG
AGGACGGCAA
CATCCTGGGG
CACAAGCTGG
AGTACAACTA
CAACAGCCAC
AACGTCTATA
TCATGGCCGA
CAAGCAGAAG
AACGGCATCA
AGGTGAACTT
CAAGATCCGC
CACAACATCG
AGGACGGCAG
CGTGCAGCTC
GCCGACCACT
ACCAGCAGAA
CACCCCCATC
GGCGACGGCC
CCGTGCTGCT
GCCCGACAAC
CACTACCTGA
GCACCCAGTC
CGCCCTGAGC
AAAGACCCCA
ACGAGAAGCG
CGATCACATG
GTCCTGCTGG
AGTTCGTGAC
CGCCGCCGGG
ATCACTCTCG
GCATGGACGA
GCTGTACAAG
TAACCGGTCA
TCATCACCAT
CACCATTGAG
TTTAAACCCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGAAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
ACAATAGCAG
GCATGCTGGG
GATGCGGTGG
GCTCTATGGC
TTCTGAGGCG
GAAAGAACCA
GCTGGGGCTC
GATACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTAGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACACTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCGAC
GGATCGGGAG
ATCGATCTCC
CGATCCCCTA
GGGTCGACTC
TCAGTACAAT
CTGCTCTGAT
GCCGCATAGT
TAAGCCAGTA
TCTGCTCCCT
GCTTGTGTGT
TGGAGGTCGC
TGAGTAGTGC
GCGAGCAAAA
TTTAAGCTAC
AACAAGGCAA
GGCTTGACCG
ACAATTGCAT
GAAGAATCTG
CTTAGGGTTA
GGCGTTTTGC
GCTGCTTCGC
GATGTACGGG
CCAGATATAG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GTAAGTGCCG
TGTGTGGTTC
CCGCGGGCCT
GGCCTCTTTA
CGGGTTATGG
CCCTTGCGTG
CCTTGAATTA
CTTCCACCTG
GCTGCAGTAC
GTGATTCTTG
ATCCCGAGCT
TCGGGTTGGA
AGTGGGTGGG
AGAGTTCGAG
GCCTTGCGCT
TAAGGAGCCC
CTTCGCCTCG
TGCTTGAGTT
GAGGCCTGGC
CTGGGCGCTG
GGGCCGCCGC
GTGCGAATCT
GGTGGCACCT
TCGCGCCTGT
CTCGCTGCTT
TCGATAAGTC
TCTAGCCATT
TAAAATTTTT
GATGACCTGC
TGCGACGCTT
TTTTTCTGGC
AAGATAGTCT
TGTAAATGCG
GGCCAAGATC
TGCACACTGG
TATTTCGGTT
TTTGGGGCCG
CGGGCGGCGA
CGGGGCCCGT
GCGTCCCAGC
GCACATGTTC
GGCGAGGCGG
GGCCTGCGAG
CGCGGCCACC
GAGAATCGGA
CGGGGGTAGT
CTCAAGCTGG
CCGGCCTGCT
CTGGTGCCTG
GCCTCGCGCC
GCCGTGTATC
GCCCCGCCCT
GGGCGGCAAG
GCTGGCCCGG
TCGGCACCAG
TTGCGTGAGC
GGAAAGATGG
CCGCTTCCCG
GCCCTGCTGC
AGGGAGCTCA
AAATGGAGGA
CGCGGCGCTC
GGGAGAGCGG
GCGGGTGAGT
CACCCACACA
AAGGAAAAGG
GCCTTTCCGT
CCTCAGCCGT
CGCTTCATGT
GACTCCACGG
AGTACCGGGC
GCCGTCCAGG
CACCTCGATT
AGTTCTCGAG
CTTTTGGAGT
ACGTCGTCTT
TAGGTTGGGG
GGAGGGGTTT
TATGCGATGG
AGTTTCCCCA
CACTGAGTGG
GTGGAGACTG
AAGTTAGGCC
AGCTTGGCAC
TTGATGTAAT
TCTCCTTGGA
ATTTGCCCTT
TTTGAGTTTG
GATCTTGGTT
CATTCTCAAG
CCTCAGACAG
TGGTTCAAAG
TTTTTTTCTT
CCATTTCAGG
TGTCGTGAGC
TAATACGACT
CACTATAGGG
AGAGCCGCCA
CCATGAAACG
GACAGCCGAC
GGAAGCGAGT
TCGAGTCA