名称: | pLV3-CMV-FNDC1(human-repeat-opt)(N149A)-3×FLAG-CopGFP-Puro |
---|---|
别称: | - |
基因长: | 5682bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGG
CCCCCGAGGC
CGGGGCGACC
CTGCGCGCGC
CGCGCCGGCT
GTCCTGGGCG
GCGCTGCTGC
TCTTGGCCGC
GCTGCTCCCC
GTCGCCTCCT
CGGCGGCGGC
CTCAGTTGAC
CACCCACTGA
AGCCAAGGCA
TGTGAAACTG
CTGTCCACTA
AAATGGGCCT
GAAAGTCACG
TGGGACCCAC
CCAAAGATGC
TACCAGTAGA
CCTGTGGAGC
ATTACAACAT
TGCCTATGGG
AAGTCACTGA
AAAGTCTTAA
ATACATCAAG
GTGAATGCGG
AGACATACTC
CTTCCTTATT
GAGGATGTGG
AGCCGGGGGT
AGTGTACTTT
GTGCTGCTTA
CTGCAGAAAA
CCACAGTGGA
GTGAGCCGTC
CTGTTTACAG
AGCTGAAAGC
CCACCTGGAG
GTGAATGGAT
CGAGATTGAT
GGTTTTCCCA
TTAAGGGTCC
AGGACCATTT
GCTGAAACCG
TCACAGAAAA
GGAAGTGCCC
AACAAGCCCT
TGCGTGTGCG
TGTCCGGTCC
TCAGATGACA
GGCTGTCCGT
TGCGTGGAAG
GCACCACGCC
TGTCTGGAGC
CAAGAGTCCA
CGCAGATCAC
GGGGTTTTCT
CCTGGGCTAC
GGGGAGAGTG
GCCGGAAGAT
GAATTATGTT
CCACTGACAA
GAGATGAACG
GACACACGAA
ATTAAAAAGC
TAGCCTCGGA
ATCCGTGTAT
GTGGTCTCCC
TGCAGTCCAT
GAACTCTCAG
GGCCGGAGCC
AACCAGTCTA
CAGGGCTGCC
CTAACAAAGC
GAAAGATTTC
AGAAGAGGAC
GAATTGGATG
TACCTGACGA
CATCAGCGTC
CGGGTTATGT
CATCTCAGTC
TGTGCTTGTG
TCCTGGGTGG
ATCCTGTTCT
GGAAAAACAG
AAGAAAGTTG
TTGCATCAAG
ACAGTACACC
GTGCGCTATC
GAGAGAAGGG
GGAATTGGCC
AGGTGGGATT
ATAAGCAGAT
CGCTAACAGG
CGTGTGCTGA
TTGAGAACCT
GATTCCAGAC
ACTGTGTATG
AATTTGCAGT
CCGTATTTCA
CAGGGTGAAA
GAGATGGCAA
ATGGAGTACG
TCAGTCTTCC
AAAGAACACC
AGAATCTGCC
CCTACCACAG
CTCCTGAAAA
CTTGAACGTC
TGGCCAGTCA
ATGGCAAACC
TACAGTTGTC
GCTGCATCTT
GGGATGCGCT
ACCAGAGACT
GAGGGGAAAG
TGAAAGAATA
CATTCTTTCA
TACGCCCCGG
CTCTCAAACC
ATTTGGAGCA
AAGTCCCTCA
CCTATCCTGG
AGACACTACT
TCTGCCCTGG
TGGATGGTCT
GCAGCCTGGG
GAACGCTATC
TTTTCAAAAT
CCGGGCCACA
AACAGGAGAG
GCCTGGGACC
TCACTCCAAA
GCCTTCATTG
TCGCTATGCC
AACAACCAGT
AAGGCGGATG
TTGAGCAGAA
CACGGAGGAC
AATGGGAAAC
CCGAAAAACC
TGAGCCTTCC
TCACCTTCTC
CCAGAGCTCC
AGCTTCCTCC
CAACACCCCT
CTGTGCCTGC
TTCTCCCCAA
GGGAGAAATG
CCAAGGACCT
TCTTCTTGAC
TTGAAGAACA
AAATATTGGC
TAATGGTGGG
GCGCCCCGAA
AACCCCAGCT
TCGCGCCAAG
AAGGCAGAGG
AGCTGGATCT
TCAGTCGACA
GAAATCACTG
GGGAGGAGGA
GCTGGGTTCC
CGGGAGGACT
CGCCCATGTC
ACCCTCAGAC
ACCCAAGACC
AGAAACGGAC
CCTGAGGCCG
CCAAGTAGAC
ACGGCCACTC
GGTGGTTGCT
CCCGGCAGGA
CTGCAGTGAG
GGCCCGGATG
CCAGCGCTGC
CCCGAAGGGA
AGGCGTAGAT
AAGCCTGGCT
TTTCCCTGGC
CACGCAGCCC
CGCCCAGGGG
CGCCCCCCTC
GGCTTCGGCC
TCTCCTGCCC
ACCACGCGTC
CACCCAGGGC
ACCTCTCATC
GTCCTTCCCT
GCCTGCCAGC
TTGAATGACA
ACGACTTGGT
GGACTCAGAC
GAAGATGAGC
GCGCTGTGGG
CTCCCTCCAC
CCCAAGGGCG
CCTTCGCCCA
GCCCCGGCCA
GCCCTGTCCC
CCAGCCGCCA
GTCCCCGTCC
AGCGTTCTCC
GCGACAGAAG
CTCTGTGCAC
CCCGGCGCAA
AGCCAGCCTC
GCCGGCCCGG
AGGACCCCCC
ATTCAGGGGC
CGCAGAGGAA
GATTCCAGTG
CCTCAGCCCC
ACCCTCAAGA
CTTTCTCCAC
CCCATGGGGG
ATCATCTCGG
CTGCTGCCCA
CCCAGCCACA
CCTGAGCTCT
CCACTTTCCA
AGGGCGGGAA
GGATGGTGAG
GACGCCCCAG
CCACCAACTC
CAATGCGCCA
TCACGGTCCA
CCATGTCCTC
CTCCGTCTCT
TCTCATCTCT
CGTCCAGGAC
GCAGGTCTCT
GAGGGAGCGG
AGGCTTCTGA
TGGTGAAAGC
CACGGTGACG
GCGATAGGGA
AGACGGCGGA
AGGCAGGCGG
AGGCCACGGC
CCAGACGCTG
CGGGCCCGGC
CTGCCTCTGG
ACACTTCCAT
TTGCTCAGAC
ACAAACCCTT
TGCTGCCAAC
GGGAGGTCTC
CAAGCAGGTT
CAGCATTGGG
CGGGGACCTC
GGCTGCAGCC
CTCCAGCTCC
CCACAGTCGA
CTGTGCCCTC
CCGAGCCCAC
CCCAGGGTTC
CCTCTCACTC
TGATTCCCAC
CCTAAGCTTA
GCTCAGGTAT
CCATGGAGAC
GAGGAGGATG
AGAAGCCGCT
TCCTGCCACC
GTTGTCAATG
ACCACGTGCC
TTCCTCCTCC
AGGCAGCCCA
TCTCCCGGGG
CTGGGAGGAC
TTAAGGAGAA
GCCCGCAGAG
AGGGGCCAGC
CTGCATCGGA
AGGAACCCAT
CCCAGAGAAC
CCCAAATCCA
CAGGGGCAGA
TACACATCCT
CAGGGCAAGT
ACTCCTCCCT
GGCCTCCAAG
GCTCAGGATG
TTCAACAGAG
CACAGACGCG
GACACGGAGG
GTCATTCTCC
CAAAGCACAG
CCAGGGTCCA
CAGACCGCCA
CGCGTCCCCT
GCTCGTCCGC
CCGCAGCACG
GTCACAGCAG
CATCCCAGTG
TTCCCAGAAG
GATGACACCC
GGCCGGGCCC
CACAACAGCA
GCCCCCTCCT
CCCGTCGCCA
CGTCCCAGCA
CCACCCGGGA
CCCCAGAGCA
GAGACGCGGG
TCGGTCACCT
TCCCAGCCCA
GGCTCTCACT
GACCCAGGCC
GGGCGGCCCC
GCCCCACGTC
GCAGGGCCGC
TCCCACTCCT
CCTCGGACCC
TTACACGGCG
AGCTCCAGAG
GGATGCTCCC
CACGGCCCTC
CAGAACCAGG
ACGAGGATGC
CCAGGGCAGC
TACGACGACG
ACAGCACAGA
AGTCGAGGCC
CAGGATGTGC
GGGCCCCCGC
GCACGCCGCG
CGCGCCAAGG
AGGCAGCTGC
GTCCCTTCCC
AAGCACCAGC
AGGTGGAGTC
TCCCACAGGC
GCAGGGGCAG
GTGGCGACCA
CAGGTCCCAG
CGCGGACATG
CGGCCTCCCC
CGCCAGGCCC
AGCCGACCCG
GCGGCCCCCA
GTCCCGCGCC
CGGGTACCCA
GCAGGGCAGC
GCCGGGGAAG
TCGGAGCCTC
CTTCCAAGCG
GCCCCTGTCC
TCCAAGTCCC
AGCAGTCGGT
CTCAGCCGAG
GACGACGAGG
AGGAGGACGC
GGGATTTTTT
AAAGGCGGGA
AAGAAGACCT
TCTGTCTTCC
TCTGTGCCAA
AGTGGCCCTC
TTCCTCCACT
CCCAGGGGCG
GCAAAGACGC
CGATGGGAGC
CTCGCCAAGG
AAGAGAGGGA
GCCTGCCATC
GCGCTTGCCC
CTCGCGGAGG
GAGCCTGGCT
CCTGTGAAGC
GACCTCTCCC
CCCACCTCCA
GGCAGCTCCC
CCAGGGCCTC
CCACGTCCCT
TCCCGACTGC
CGCCTCGCAG
CGCTGCCACC
GTGAGCCCCG
TCGCGGGCAC
CCACCCCTGG
CCGCAGTACA
CCACGCGCGC
CCCACCTGGC
CACTTCTCCA
CCACCCCGAT
GCTGTCCTTG
CGCCAGAGGA
TGATGCATGC
CAGATTCCGT
AACCCTCTCT
CCCGACAGCC
TGCCAGACCC
TCTTACAGAC
AAGGTTATAA
TGGCAGACCA
AATGTAGAAG
GGAAAGTCCT
TCCTGGTAGT
AATGGAAAAC
CGAATGGACA
GAGAATTATC
AATGGCCCTC
AAGGAACAAA
GTGGGTTGTG
GACCTTGATC
GTGGGTTAGT
ATTGAATGCA
GAAGGAAGGT
ACCTCCAAGA
TTCACATGGA
AATCCTCTTC
GGATTAAACT
AGGAGGAGAT
GGTCGAACCA
TTGTAGATCT
GGAAGGGACC
CCCGTGGTGA
GTCCTGACGG
CCTCCCACTC
TTTGGGCAGG
GGCGACATGG
CACACCTCTG
GCCAATGCCC
AAGATAAGCC
AATTTTGAGT
CTTGGAGGAA
AGCCGCTGGT
GGGCTTGGAG
GTCATCAAAA
AAACCACCCA
TCCCCCTACC
ACTACCATGC
AGCCCACCAC
TACTACGACG
CCCCTGCCTA
CCACTACAAC
CCCGAGGCCC
ACCACTGCCA
CCACCAGGCG
GACGACCACA
ACCAGGCGGA
CAACCACCAG
ACGACCGACT
ACGACTGTGA
GGACCACCAC
ACGCACCACC
ACCACAACGA
CCCCCACTCC
AACCACCCCG
ATACCAACGT
GTCCCCCTGG
GACCTTGGAA
CGGCACGACG
ATGATGGCAA
CCTGATAATG
AGCTCCAATG
GGATCCCAGA
GTGCTACGCT
GAAGAAGATG
AGTTCTCAGG
CTTGGAGACT
GACACTGCAG
TACCTACGGA
AGAGGCCTAC
GTTATATATG
ATGAAGATTA
TGAATTTGAG
ACGTCAAGGC
CACCAACCAC
CACTGAGCCT
TCGACCACTG
CTACCACACC
GAGGGTGATC
CCAGAGGAAG
GCGCCATCAG
TTCCTTTCCT
GAAGAAGAAT
TTGATCTGGC
TGGAAGGAAA
CGATTTGTTG
CTCCTTACGT
GACGTACCTA
AATAAAGACC
CATCAGCCCC
GTGCTCTCTG
ACTGATGCAC
TGGATCACTT
CCAAGTGGAC
AGCCTGGATG
AAATCATCCC
CAATGACCTG
AAGAAGAGTG
ACCTGCCTCC
CCAGCATGCT
CCCCGCAACA
TCACCGTGGT
GGCCGTGGAA
GGTTGCCACT
CATTTGTCAT
TGTGGACTGG
GACAAAGCCA
CCCCAGGAGA
TGTGGTCACA
GGTTACTTGG
TTTACAGTGC
ATCCTATGAA
GACTTCATCA
GGAACAAGTG
GTCCACTCAA
GCTTCATCAG
TAACTCACTT
GCCCATTGAG
AACCTAAAGC
CCAACACGAG
GTATTATTTT
AAAGTGCAAG
CACAAAATCC
TCATGGCTAC
GGACCTATCA
GCCCTTCGGT
CTCATTTGTC
ACCGAATCAG
ATAATCCTCT
GCTTGTTGTG
AGGCCCCCAG
GCGGTGAGCC
TATCTGGATC
CCATTCGCTT
TCAAACATGA
TCCCAGCTAC
ACGGACTGCC
ATGGACGGCA
ATATGTGAAG
CGCACGTGGT
ATCGAAAGTT
CGTGGGAGTT
GTTCTTTGTA
ATTCACTGAG
GTATAAAATC
TACCTCAGTG
ACAACCTGAA
AGATACATTC
TACAGCATTG
GAGACAGCTG
GGGAAGAGGT
GAAGACCATT
GCCAATTTGT
GGATTCACAC
CTTGATGGAA
GAACAGGGCC
TCAGTCCTAT
GTAGAAGCCC
TCCCTACTAT
TCAAGGCTAC
TATCGCCAGT
ATCGTCAGGA
GCCTGTCAGG
TTTGGGAACA
TCGGCTTCGG
AACCCCCTAC
TACTATGTGG
GCTGGTACGA
GTGTGGGGTC
TCCATCCCTG
GAAAGTGGGG
ATCCGACTAC
AAAGACCATG
ACGGTGATTA
TAAAGATCAT
GACATCGACT
ACAAGGATGA
CGATGACAAG
TAGCGGCCGC
GAAGGATCTG
CGATCGCTCC
GGTGCCCGTC
AGTGGGCAGA
GCGCACATCG
CCCACAGTCC
CCGAGAAGTT
GGGGGGAGGG
GTCGGCAATT
GAACGGGTGC
CTAGAGAAGG
TGGCGCGGGG
TAAACTGGGA
AAGTGATGTC
GTGTACTGGC
TCCGCCTTTT
TCCCGAGGGT
GGGGGAGAAC
CGTATATAAG
TGCAGTAGTC
GCCGTGAACG
TTCTTTTTCG
CAACGGGTTT
GCCGCCAGAA
CACAGCTGAA
GCTTCGAGGG
GCTCGCATCT
CTCCTTCACG
CGCCCGCCGC
CCTACCTGAG
GCCGCCATCC
ACGCCGGTTG
AGTCGCGTTC
TGCCGCCTCC
CGCCTGTGGT
GCCTCCTGAA
CTGCGTCCGC
CGTCTAGGTA
AGTTTAAAGC
TCAGGTCGAG
ACCGGGCCTT
TGTCCGGCGC
TCCCTTGGAG
CCTACCTAGA
CTCAGCCGGC
TCTCCACGCT
TTGCCTGACC
CTGCTTGCTC
AACTCTACGT
CTTTGTTTCG
TTTTCTGTTC
TGCGCCGTTA
CAGATCCAAG
CTGTGACCGG
CGCCTACGCT
AGACGCCACC
ATGGAGAGCG
ACGAGAGCGG
CCTGCCCGCC
ATGGAGATCG
AGTGCCGCAT
CACCGGCACC
CTGAACGGCG
TGGAGTTCGA
GCTGGTGGGC
GGCGGAGAGG
GCACCCCCAA
GCAGGGCCGC
ATGACCAACA
AGATGAAGAG
CACCAAAGGC
GCCCTGACCT
TCAGCCCCTA
CCTGCTGAGC
CACGTGATGG
GCTACGGCTT
CTACCACTTC
GGCACCTACC
CCAGCGGCTA
CGAGAACCCC
TTCCTGCACG
CCATCAACAA
CGGCGGCTAC
ACCAACACCC
GCATCGAGAA
GTACGAGGAC
GGCGGCGTGC
TGCACGTGAG
CTTCAGCTAC
CGCTACGAGG
CCGGCCGCGT
GATCGGCGAC
TTCAAGGTGG
TGGGCACCGG
CTTCCCCGAG
GACAGCGTGA
TCTTCACCGA
CAAGATCATC
CGCAGCAACG
CCACCGTGGA
GCACCTGCAC
CCCATGGGCG
ATAACGTGCT
GGTGGGCAGC
TTCGCCCGCA
CCTTCAGCCT
GCGCGACGGC
GGCTACTACA
GCTTCGTGGT
GGACAGCCAC
ATGCACTTCA
AGAGCGCCAT
CCACCCCAGC
ATCCTGCAGA
ACGGGGGCCC
CATGTTCGCC
TTCCGCCGCG
TGGAGGAGCT
GCACAGCAAC
ACCGAGCTGG
GCATCGTGGA
GTACCAGCAC
GCCTTCAAGA
CCCCCATCGC
CTTCGCCAGA
TCCCGCGCTC
AGTCGTCCAA
TTCTGCCGTG
GACGGCACCG
CCGGACCCGG
CTCCACCGGA
TCTCGCGAGG
GCAGAGGAAG
TCTTCTAACA
TGCGGTGACG
TGGAGGAGAA
TCCCGGCCCT
ATGACCGAGT
ACAAGCCCAC
GGTGCGCCTC
GCCACCCGCG
ACGACGTCCC
CAGGGCCGTA
CGCACCCTCG
CCGCCGCGTT
CGCCGACTAC
CCCGCCACGC
GCCACACCGT
CGATCCGGAC
CGCCACATCG
AGCGGGTCAC
CGAGCTGCAA
GAACTCTTCC
TCACGCGCGT
CGGGCTCGAC
ATCGGCAAGG
TGTGGGTCGC
GGACGACGGC
GCCGCGGTGG
CGGTCTGGAC
CACGCCGGAG
AGCGTCGAAG
CGGGGGCGGT
GTTCGCCGAG
ATCGGCCCGC
GCATGGCCGA
GTTGAGCGGT
TCCCGGCTGG
CCGCGCAGCA
ACAGATGGAA
GGCCTCCTGG
CGCCGCACCG
GCCCAAGGAG
CCCGCGTGGT
TCCTGGCCAC
CGTCGGCGTC
TCGCCCGACC
ACCAGGGCAA
GGGTCTGGGC
AGCGCCGTCG
TGCTCCCCGG
AGTGGAGGCG
GCCGAGCGCG
CCGGGGTGCC
CGCCTTCCTG
GAGACCTCCG
CGCCCCGCAA
CCTCCCCTTC
TACGAGCGGC
TCGGCTTCAC
CGTCACCGCC
GACGTCGAGG
TGCCCGAAGG
ACCGCGCACC
TGGTGCATGA
CCCGCAAGCC
CGGTGCCTGA
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAATCAT
CGTCCTTTCC
TTGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGGTACCTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ATAAGATCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTAG
TAGTTCATGT
CATCTTATTA
TTCAGTATTT
ATAACTTGCA
AAGAAATGAA
TATCAGAGAG
TGAGAGGAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGC
TCTAGCTATC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGACT
TTTGCAGAGA
CCAAATTCGT
AATCATGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGG
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCTAAGAA
ACCATTATTA
TCATGACATT
AACCTATAAA
AATAGGCGTA
TCACGAGGCC
CTTTCGTCTC
GCGCGTTTCG
GTGATGACGG
TGAAAACCTC
TGACACATGC
AGCTCCCGGA
GACGGTCACA
GCTTGTCTGT
AAGCGGATGC
CGGGAGCAGA
CAAGCCCGTC
AGGGCGCGTC
AGCGGGTGTT
GGCGGGTGTC
GGGGCTGGCT
TAACTATGCG
GCATCAGAGC
AGATTGTACT
GAGAGTGCAC
CATATGCGGT
GTGAAATACC
GCACAGATGC
GTAAGGAGAA
AATACCGCAT
CAGGCGCCAT
TCGCCATTCA
GGCTGCGCAA
CTGTTGGGAA
GGGCGATCGG
TGCGGGCCTC
TTCGCTATTA
CGCCAGCTGG
CGAAAGGGGG
ATGTGCTGCA
AGGCGATTAA
GTTGGGTAAC
GCCAGGGTTT
TCCCAGTCAC
GACGTTGTAA
AACGACGGCC
AGTGCCAAGC
TG