名称: | pLV3-U6-Cd200(mouse)-sgRNA2-Cas9-Hyg |
---|---|
别称: | AGGCTGGATTACAACCCCAT |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Hyg |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLentiCRISPRV2-Hygro |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGCGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GCGCGTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TGGCGCCCGA
ACAGGGACTT
GAAAGCGAAA
GGGAAACCAG
AGGAGCTCTC
TCGACGCAGG
ACTCGGCTTG
CTGAAGCGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGTACGCCA
AAAATTTTGA
CTAGCGGAGG
CTAGAAGGAG
AGAGATGGGT
GCGAGAGCGT
CAGTATTAAG
CGGGGGAGAA
TTAGATCGCG
ATGGGAAAAA
ATTCGGTTAA
GGCCAGGGGG
AAAGAAAAAA
TATAAATTAA
AACATATAGT
ATGGGCAAGC
AGGGAGCTAG
AACGATTCGC
AGTTAATCCT
GGCCTGTTAG
AAACATCAGA
AGGCTGTAGA
CAAATACTGG
GACAGCTACA
ACCATCCCTT
CAGACAGGAT
CAGAAGAACT
TAGATCATTA
TATAATACAG
TAGCAACCCT
CTATTGTGTG
CATCAAAGGA
TAGAGATAAA
AGACACCAAG
GAAGCTTTAG
ACAAGATAGA
GGAAGAGCAA
AACAAAAGTA
AGACCACCGC
ACAGCAAGCG
GCCGCTGATC
TTCAGACCTG
GAGGAGGAGA
TATGAGGGAC
AATTGGAGAA
GTGAATTATA
TAAATATAAA
GTAGTAAAAA
TTGAACCATT
AGGAGTAGCA
CCCACCAAGG
CAAAGAGAAG
AGTGGTGCAG
AGAGAAAAAA
GAGCAGTGGG
AATAGGAGCT
TTGTTCCTTG
GGTTCTTGGG
AGCAGCAGGA
AGCACTATGG
GCGCAGCGTC
AATGACGCTG
ACGGTACAGG
CCAGACAATT
ATTGTCTGGT
ATAGTGCAGC
AGCAGAACAA
TTTGCTGAGG
GCTATTGAGG
CGCAACAGCA
TCTGTTGCAA
CTCACAGTCT
GGGGCATCAA
GCAGCTCCAG
GCAAGAATCC
TGGCTGTGGA
AAGATACCTA
AAGGATCAAC
AGCTCCTGGG
GATTTGGGGT
TGCTCTGGAA
AACTCATTTG
CACCACTGCT
GTGCCTTGGA
ATGCTAGTTG
GAGTAATAAA
TCTCTGGAAC
AGATTTGGAA
TCACACGACC
TGGATGGAGT
GGGACAGAGA
AATTAACAAT
TACACAAGCT
TAATACACTC
CTTAATTGAA
GAATCGCAAA
ACCAGCAAGA
AAAGAATGAA
CAAGAATTAT
TGGAATTAGA
TAAATGGGCA
AGTTTGTGGA
ATTGGTTTAA
CATAACAAAT
TGGCTGTGGT
ATATAAAATT
ATTCATAATG
ATAGTAGGAG
GCTTGGTAGG
TTTAAGAATA
GTTTTTGCTG
TACTTTCTAT
AGTGAATAGA
GTTAGGCAGG
GATATTCACC
ATTATCGTTT
CAGACCCACC
TCCCAACCCC
GAGGGGACCC
GACAGGCCCG
AAGGAATAGA
AGAAGAAGGT
GGAGAGAGAG
ACAGAGACAG
ATCCATTCGA
TTAGTGAACG
GATCGGCACT
GCGTGCGCCA
ATTCTGCAGA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
AGTTTGGTTA
ATTAAGGTAC
CGAGGGCCTA
TTTCCCATGA
TTCCTTCATA
TTTGCATATA
CGATACAAGG
CTGTTAGAGA
GATAATTAGA
ATTAATTTGA
CTGTAAACAC
AAAGATATTA
GTACAAAATA
CGTGACGTAG
AAAGTAATAA
TTTCTTGGGT
AGTTTGCAGT
TTTAAAATTA
TGTTTTAAAA
TGGACTATCA
TATGCTTACC
GTAACTTGAA
AGTATTTCGA
TTTCTTGGCT
TTATATATCT
TGTGGAAAGG
ACGAAACACC
GAGGCTGGAT
TACAACCCCA
TGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TTTGAATTCG
CTAGCTAGGT
CTTGAAAGGA
GTGGGAATTG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGATCC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GACCGGTTCT
AGAGCGCTGC
CACCATGGAC
AAGAAGTACA
GCATCGGCCT
GGACATCGGC
ACCAACTCTG
TGGGCTGGGC
CGTGATCACC
GACGAGTACA
AGGTGCCCAG
CAAGAAATTC
AAGGTGCTGG
GCAACACCGA
CCGGCACAGC
ATCAAGAAGA
ACCTGATCGG
AGCCCTGCTG
TTCGACAGCG
GCGAAACAGC
CGAGGCCACC
CGGCTGAAGA
GAACCGCCAG
AAGAAGATAC
ACCAGACGGA
AGAACCGGAT
CTGCTATCTG
CAAGAGATCT
TCAGCAACGA
GATGGCCAAG
GTGGACGACA
GCTTCTTCCA
CAGACTGGAA
GAGTCCTTCC
TGGTGGAAGA
GGATAAGAAG
CACGAGCGGC
ACCCCATCTT
CGGCAACATC
GTGGACGAGG
TGGCCTACCA
CGAGAAGTAC
CCCACCATCT
ACCACCTGAG
AAAGAAACTG
GTGGACAGCA
CCGACAAGGC
CGACCTGCGG
CTGATCTATC
TGGCCCTGGC
CCACATGATC
AAGTTCCGGG
GCCACTTCCT
GATCGAGGGC
GACCTGAACC
CCGACAACAG
CGACGTGGAC
AAGCTGTTCA
TCCAGCTGGT
GCAGACCTAC
AACCAGCTGT
TCGAGGAAAA
CCCCATCAAC
GCCAGCGGCG
TGGACGCCAA
GGCCATCCTG
TCTGCCAGAC
TGAGCAAGAG
CAGACGGCTG
GAAAATCTGA
TCGCCCAGCT
GCCCGGCGAG
AAGAAGAATG
GCCTGTTCGG
AAACCTGATT
GCCCTGAGCC
TGGGCCTGAC
CCCCAACTTC
AAGAGCAACT
TCGACCTGGC
CGAGGATGCC
AAACTGCAGC
TGAGCAAGGA
CACCTACGAC
GACGACCTGG
ACAACCTGCT
GGCCCAGATC
GGCGACCAGT
ACGCCGACCT
GTTTCTGGCC
GCCAAGAACC
TGTCCGACGC
CATCCTGCTG
AGCGACATCC
TGAGAGTGAA
CACCGAGATC
ACCAAGGCCC
CCCTGAGCGC
CTCTATGATC
AAGAGATACG
ACGAGCACCA
CCAGGACCTG
ACCCTGCTGA
AAGCTCTCGT
GCGGCAGCAG
CTGCCTGAGA
AGTACAAAGA
GATTTTCTTC
GACCAGAGCA
AGAACGGCTA
CGCCGGCTAC
ATTGACGGCG
GAGCCAGCCA
GGAAGAGTTC
TACAAGTTCA
TCAAGCCCAT
CCTGGAAAAG
ATGGACGGCA
CCGAGGAACT
GCTCGTGAAG
CTGAACAGAG
AGGACCTGCT
GCGGAAGCAG
CGGACCTTCG
ACAACGGCAG
CATCCCCCAC
CAGATCCACC
TGGGAGAGCT
GCACGCCATT
CTGCGGCGGC
AGGAAGATTT
TTACCCATTC
CTGAAGGACA
ACCGGGAAAA
GATCGAGAAG
ATCCTGACCT
TCCGCATCCC
CTACTACGTG
GGCCCTCTGG
CCAGGGGAAA
CAGCAGATTC
GCCTGGATGA
CCAGAAAGAG
CGAGGAAACC
ATCACCCCCT
GGAACTTCGA
GGAAGTGGTG
GACAAGGGCG
CTTCCGCCCA
GAGCTTCATC
GAGCGGATGA
CCAACTTCGA
TAAGAACCTG
CCCAACGAGA
AGGTGCTGCC
CAAGCACAGC
CTGCTGTACG
AGTACTTCAC
CGTGTATAAC
GAGCTGACCA
AAGTGAAATA
CGTGACCGAG
GGAATGAGAA
AGCCCGCCTT
CCTGAGCGGC
GAGCAGAAAA
AGGCCATCGT
GGACCTGCTG
TTCAAGACCA
ACCGGAAAGT
GACCGTGAAG
CAGCTGAAAG
AGGACTACTT
CAAGAAAATC
GAGTGCTTCG
ACTCCGTGGA
AATCTCCGGC
GTGGAAGATC
GGTTCAACGC
CTCCCTGGGC
ACATACCACG
ATCTGCTGAA
AATTATCAAG
GACAAGGACT
TCCTGGACAA
TGAGGAAAAC
GAGGACATTC
TGGAAGATAT
CGTGCTGACC
CTGACACTGT
TTGAGGACAG
AGAGATGATC
GAGGAACGGC
TGAAAACCTA
TGCCCACCTG
TTCGACGACA
AAGTGATGAA
GCAGCTGAAG
CGGCGGAGAT
ACACCGGCTG
GGGCAGGCTG
AGCCGGAAGC
TGATCAACGG
CATCCGGGAC
AAGCAGTCCG
GCAAGACAAT
CCTGGATTTC
CTGAAGTCCG
ACGGCTTCGC
CAACAGAAAC
TTCATGCAGC
TGATCCACGA
CGACAGCCTG
ACCTTTAAAG
AGGACATCCA
GAAAGCCCAG
GTGTCCGGCC
AGGGCGATAG
CCTGCACGAG
CACATTGCCA
ATCTGGCCGG
CAGCCCCGCC
ATTAAGAAGG
GCATCCTGCA
GACAGTGAAG
GTGGTGGACG
AGCTCGTGAA
AGTGATGGGC
CGGCACAAGC
CCGAGAACAT
CGTGATCGAA
ATGGCCAGAG
AGAACCAGAC
CACCCAGAAG
GGACAGAAGA
ACAGCCGCGA
GAGAATGAAG
CGGATCGAAG
AGGGCATCAA
AGAGCTGGGC
AGCCAGATCC
TGAAAGAACA
CCCCGTGGAA
AACACCCAGC
TGCAGAACGA
GAAGCTGTAC
CTGTACTACC
TGCAGAATGG
GCGGGATATG
TACGTGGACC
AGGAACTGGA
CATCAACCGG
CTGTCCGACT
ACGATGTGGA
CCATATCGTG
CCTCAGAGCT
TTCTGAAGGA
CGACTCCATC
GACAACAAGG
TGCTGACCAG
AAGCGACAAG
AACCGGGGCA
AGAGCGACAA
CGTGCCCTCC
GAAGAGGTCG
TGAAGAAGAT
GAAGAACTAC
TGGCGGCAGC
TGCTGAACGC
CAAGCTGATT
ACCCAGAGAA
AGTTCGACAA
TCTGACCAAG
GCCGAGAGAG
GCGGCCTGAG
CGAACTGGAT
AAGGCCGGCT
TCATCAAGAG
ACAGCTGGTG
GAAACCCGGC
AGATCACAAA
GCACGTGGCA
CAGATCCTGG
ACTCCCGGAT
GAACACTAAG
TACGACGAGA
ATGACAAGCT
GATCCGGGAA
GTGAAAGTGA
TCACCCTGAA
GTCCAAGCTG
GTGTCCGATT
TCCGGAAGGA
TTTCCAGTTT
TACAAAGTGC
GCGAGATCAA
CAACTACCAC
CACGCCCACG
ACGCCTACCT
GAACGCCGTC
GTGGGAACCG
CCCTGATCAA
AAAGTACCCT
AAGCTGGAAA
GCGAGTTCGT
GTACGGCGAC
TACAAGGTGT
ACGACGTGCG
GAAGATGATC
GCCAAGAGCG
AGCAGGAAAT
CGGCAAGGCT
ACCGCCAAGT
ACTTCTTCTA
CAGCAACATC
ATGAACTTTT
TCAAGACCGA
GATTACCCTG
GCCAACGGCG
AGATCCGGAA
GCGGCCTCTG
ATCGAGACAA
ACGGCGAAAC
CGGGGAGATC
GTGTGGGATA
AGGGCCGGGA
TTTTGCCACC
GTGCGGAAAG
TGCTGAGCAT
GCCCCAAGTG
AATATCGTGA
AAAAGACCGA
GGTGCAGACA
GGCGGCTTCA
GCAAAGAGTC
TATCCTGCCC
AAGAGGAACA
GCGATAAGCT
GATCGCCAGA
AAGAAGGACT
GGGACCCTAA
GAAGTACGGC
GGCTTCGACA
GCCCCACCGT
GGCCTATTCT
GTGCTGGTGG
TGGCCAAAGT
GGAAAAGGGC
AAGTCCAAGA
AACTGAAGAG
TGTGAAAGAG
CTGCTGGGGA
TCACCATCAT
GGAAAGAAGC
AGCTTCGAGA
AGAATCCCAT
CGACTTTCTG
GAAGCCAAGG
GCTACAAAGA
AGTGAAAAAG
GACCTGATCA
TCAAGCTGCC
TAAGTACTCC
CTGTTCGAGC
TGGAAAACGG
CCGGAAGAGA
ATGCTGGCCT
CTGCCGGCGA
ACTGCAGAAG
GGAAACGAAC
TGGCCCTGCC
CTCCAAATAT
GTGAACTTCC
TGTACCTGGC
CAGCCACTAT
GAGAAGCTGA
AGGGCTCCCC
CGAGGATAAT
GAGCAGAAAC
AGCTGTTTGT
GGAACAGCAC
AAGCACTACC
TGGACGAGAT
CATCGAGCAG
ATCAGCGAGT
TCTCCAAGAG
AGTGATCCTG
GCCGACGCTA
ATCTGGACAA
AGTGCTGTCC
GCCTACAACA
AGCACCGGGA
TAAGCCCATC
AGAGAGCAGG
CCGAGAATAT
CATCCACCTG
TTTACCCTGA
CCAATCTGGG
AGCCCCTGCC
GCCTTCAAGT
ACTTTGACAC
CACCATCGAC
CGGAAGAGGT
ACACCAGCAC
CAAAGAGGTG
CTGGACGCCA
CCCTGATCCA
CCAGAGCATC
ACCGGCCTGT
ACGAGACACG
GATCGACCTG
TCTCAGCTGG
GAGGCGACAA
GCGACCTGCC
GCCACAAAGA
AGGCTGGACA
GGCTAAGAAG
AAGAAAGATT
ACAAAGACGA
TGACGATAAG
GGATCCGGCG
CAACAAACTT
CTCTCTGCTG
AAACAAGCCG
GAGATGTCGA
AGAGAATCCT
GGACCGAAAA
AGCCTGAACT
CACCGCGACG
TCTGTCGAGA
AGTTTCTGAT
CGAAAAGTTC
GACAGCGTGT
CCGACCTGAT
GCAGCTCTCG
GAGGGCGAAG
AATCTCGTGC
TTTCAGCTTC
GATGTAGGAG
GGCGTGGATA
TGTCCTGCGG
GTAAATAGCT
GCGCCGATGG
TTTCTACAAA
GATCGTTATG
TTTATCGGCA
CTTTGCATCG
GCCGCGCTCC
CGATTCCGGA
AGTGCTTGAC
ATTGGGGAAT
TCAGCGAGAG
CCTGACCTAT
TGCATCTCCC
GCCGTGCACA
GGGTGTCACG
TTGCAAGACC
TGCCTGAAAC
CGAACTGCCC
GCTGTTCTGC
AGCCGGTCGC
GGAGGCCATG
GATGCGATCG
CTGCGGCCGA
TCTTAGCCAG
ACGAGCGGGT
TCGGCCCATT
CGGACCGCAA
GGAATCGGTC
AATACACTAC
ATGGCGTGAT
TTCATATGCG
CGATTGCTGA
TCCCCATGTG
TATCACTGGC
AAACTGTGAT
GGACGACACC
GTCAGTGCGT
CCGTCGCGCA
GGCTCTCGAT
GAGCTGATGC
TTTGGGCCGA
GGACTGCCCC
GAAGTCCGGC
ACCTCGTGCA
CGCGGATTTC
GGCTCCAACA
ATGTCCTGAC
GGACAATGGC
CGCATAACAG
CGGTCATTGA
CTGGAGCGAG
GCGATGTTCG
GGGATTCCCA
ATACGAGGTC
GCCAACATCT
TCTTCTGGAG
GCCGTGGTTG
GCTTGTATGG
AGCAGCAGAC
GCGCTACTTC
GAGCGGAGGC
ATCCGGAGCT
TGCAGGATCG
CCGCGGCTCC
GGGCGTATAT
GCTCCGCATT
GGTCTTGACC
AACTCTATCA
GAGCTTGGTT
GACGGCAATT
TCGATGATGC
AGCTTGGGCG
CAGGGTCGAT
GCGACGCAAT
CGTCCGATCC
GGAGCCGGGA
CTGTCGGGCG
TACACAAATC
GCCCGCAGAA
GCGCGGCCGT
CTGGACCGAT
GGCTGTGTAG
AAGTACTTCG
CCGATAGACG
CGTTAAGTCG
ACAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAATC
ATCGTCCTTT
CCTTGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CGTCGACTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ACAAGATCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGGGC
CCGTTTAAAC
CCGCTGATCA
GCCTCGACTG
TGCCTTCTAG
TTGCCAGCCA
TCTGTTGTTT
GCCCCTCCCC
CGTGCCTTCC
TTGACCCTGG
AAGGTGCCAC
TCCCACTGTC
CTTTCCTAAT
AAAATGAGGA
AATTGCATCG
CATTGTCTGA
GTAGGTGTCA
TTCTATTCTG
GGGGGTGGGG
TGGGGCAGGA
CAGCAAGGGG
GAGGATTGGG
AAGACAATAG
CAGGCATGCT
GGGGATGCGG
TGGGCTCTAT
GGCTTCTGAG
GCGGAAAGAA
CCAGCTGGGG
CTCTAGGGGG
TATCCCCACG
CGCCCTGTAG
CGGCGCATTA
AGCGCGGCGG
GTGTGGTGGT
TACGCGCAGC
GTGACCGCTA
CACTTGCCAG
CGCCCTAGCG
CCCGCTCCTT
TCGCTTTCTT
CCCTTCCTTT
CTCGCCACGT
TCGCCGGCTT
TCCCCGTCAA
GCTCTAAATC
GGGGGCTCCC
TTTAGGGTTC
CGATTTAGTG
CTTTACGGCA
CCTCGACCCC
AAAAAACTTG
ATTAGGGTGA
TGGTTCACGT
AGTGGGCCAT
CGCCCTGATA
GACGGTTTTT
CGCCCTTTGA
CGTTGGAGTC
CACGTTCTTT
AATAGTGGAC
TCTTGTTCCA
AACTGGAACA
ACACTCAACC
CTATCTCGGT
CTATTCTTTT
GATTTATAAG
GGATTTTGCC
GATTTCGGCC
TATTGGTTAA
AAAATGAGCT
GATTTAACAA
AAATTTAACG
CGAATTAATT
CTGTGGAATG
TGTGTCAGTT
AGGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCAGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCATAGT
CCCGCCCCTA
ACTCCGCCCA
TCCCGCCCCT
AACTCCGCCC
AGTTCCGCCC
ATTCTCCGCC
CCATGGCTGA
CTAATTTTTT
TTATTTATGC
AGAGGCCGAG
GCCGCCTCTG
CCTCTGAGCT
ATTCCAGAAG
TAGTGAGGAG
GCTTTTTTGG
AGGCCTAGGC
TTTTGCAAAA
AGCTCCCGGG
AGCTTGTATA
TCCATTTTCG
GATCTGATCA
GCACGTGTTG
ACAATTAATC
ATCGGCATAG
TATATCGGCA
TAGTATAATA
CGACAAGGTG
AGGAACTAAA
CCATGGCCAA
GTTGACCAGT
GCCGTTCCGG
TGCTCACCGC
GCGCGACGTC
GCCGGAGCGG
TCGAGTTCTG
GACCGACCGG
CTCGGGTTCT
CCCGGGACTT
CGTGGAGGAC
GACTTCGCCG
GTGTGGTCCG
GGACGACGTG
ACCCTGTTCA
TCAGCGCGGT
CCAGGACCAG
GTGGTGCCGG
ACAACACCCT
GGCCTGGGTG
TGGGTGCGCG
GCCTGGACGA
GCTGTACGCC
GAGTGGTCGG
AGGTCGTGTC
CACGAACTTC
CGGGACGCCT
CCGGGCCGGC
CATGACCGAG
ATCGGCGAGC
AGCCGTGGGG
GCGGGAGTTC
GCCCTGCGCG
ACCCGGCCGG
CAACTGCGTG
CACTTCGTGG
CCGAGGAGCA
GGACTGACAC
GTGCTACGAG
ATTTCGATTC
CACCGCCGCC
TTCTATGAAA
GGTTGGGCTT
CGGAATCGTT
TTCCGGGACG
CCGGCTGGAT
GATCCTCCAG
CGCGGGGATC
TCATGCTGGA
GTTCTTCGCC
CACCCCAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGTAT
ACCGTCGACC
TCTAGCTAGA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
C