名称: | pSIN-EF2-HA-STING1(human)-Puro |
---|---|
别称: | NM_198282.4;ERIS; hMITA; hSTING; MITA; MPYS; NET23;SAVI; STING; STING-beta; TMEM173 |
基因长: | 1140bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pSIN-EF2-Puro |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGACCGCCA
CTGCCAATTA
CCTGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGTTGGA
AGGGCTAATT
CACTCCCAAA
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTAGC
AGAACTACAC
ACCAGGGCCA
GGGGTCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCCAGAT
AAGGTAGAAG
AGGCCAATAA
AGGAGAGAAC
ACCAGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTGTTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACGTGG
CCCGAGAGCT
GCATCCGGAG
TACTTCAAGA
ACTGCTGATA
TCGAGCTTGC
TACAAGGGAC
TTTCCGCTGG
GGACTTTCCA
GGGAGGCGTG
GCCTGGGCGG
GACTGGGGAG
TGGCGAGCCC
TCAGATCCTG
CATATAAGCA
GCTGCTTTTT
GCCTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACT
TGAAAGCGAA
AGGGAAACCA
GAGGAGCTCT
CTCGACGCAG
GACTCGGCTT
GCTGAAGCGC
GCACGGCAAG
AGGCGAGGGG
CGGCGACTGG
TGAGTACGCC
AAAAATTTTG
ACTAGCGGAG
GCTAGAAGGA
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGC
GATGGGAAAA
AATTCGGTTA
AGGCCAGGGG
GAAAGAAAAA
ATATAAATTA
AAACATATAG
TATGGGCAAG
CAGGGAGCTA
GAACGATTCG
CAGTTAATCC
TGGCCTGTTA
GAAACATCAG
AAGGCTGTAG
ACAAATACTG
GGACAGCTAC
AACCATCCCT
TCAGACAGGA
TCAGAAGAAC
TTAGATCATT
ATATAATACA
GTAGCAACCC
TCTATTGTGT
GCATCAAAGG
ATAGAGATAA
AAGACACCAA
GGAAGCTTTA
GACAAGATAG
AGGAAGAGCA
AAACAAAAGT
AAGACCACCG
CACAGCAAGC
GGCCGCTGAT
CTTCAGACCT
GGAGGAGGAG
ATATGAGGGA
CAATTGGAGA
AGTGAATTAT
ATAAATATAA
AGTAGTAAAA
ATTGAACCAT
TAGGAGTAGC
ACCCACCAAG
GCAAAGAGAA
GAGTGGTGCA
GAGAGAAAAA
AGAGCAGTGG
GAATAGGAGC
TTTGTTCCTT
GGGTTCTTGG
GAGCAGCAGG
AAGCACTATG
GGCGCAGCGT
CAATGACGCT
GACGGTACAG
GCCAGACAAT
TATTGTCTGG
TATAGTGCAG
CAGCAGAACA
ATTTGCTGAG
GGCTATTGAG
GCGCAACAGC
ATCTGTTGCA
ACTCACAGTC
TGGGGCATCA
AGCAGCTCCA
GGCAAGAATC
CTGGCTGTGG
AAAGATACCT
AAAGGATCAA
CAGCTCCTGG
GGATTTGGGG
TTGCTCTGGA
AAACTCATTT
GCACCACTGC
TGTGCCTTGG
AATGCTAGTT
GGAGTAATAA
ATCTCTGGAA
CAGATTTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGCCA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAGGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
ACTTTGGATC
GATAAGCTTT
GCAAAGATGG
ATAAAGTTTT
AAACAGAGAG
GAATCTTTGC
AGCTAATGGA
CCTTCTAGGT
CTTGAAAGGA
GTGGGAATTG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GTAAGTGCCG
TGTGTGGTTC
CCGCGGGCCT
GGCCTCTTTA
CGGGTTATGG
CCCTTGCGTG
CCTTGAATTA
CTTCCACCTG
GCTGCAGTAC
GTGATTCTTG
ATCCCGAGCT
TCGGGTTGGA
AGTGGGTGGG
AGAGTTCGAG
GCCTTGCGCT
TAAGGAGCCC
CTTCGCCTCG
TGCTTGAGTT
GAGGCCTGGC
CTGGGCGCTG
GGGCCGCCGC
GTGCGAATCT
GGTGGCACCT
TCGCGCCTGT
CTCGCTGCTT
TCGATAAGTC
TCTAGCCATT
TAAAATTTTT
GATGACCTGC
TGCGACGCTT
TTTTTCTGGC
AAGATAGTCT
TGTAAATGCG
GGCCAAGATC
TGCACACTGG
TATTTCGGTT
TTTGGGGCCG
CGGGCGGCGA
CGGGGCCCGT
GCGTCCCAGC
GCACATGTTC
GGCGAGGCGG
GGCCTGCGAG
CGCGGCCACC
GAGAATCGGA
CGGGGGTAGT
CTCAAGCTGG
CCGGCCTGCT
CTGGTGCCTG
GCCTCGCGCC
GCCGTGTATC
GCCCCGCCCT
GGGCGGCAAG
GCTGGCCCGG
TCGGCACCAG
TTGCGTGAGC
GGAAAGATGG
CCGCTTCCCG
GCCCTGCTGC
AGGGAGCTCA
AAATGGAGGA
CGCGGCGCTC
GGGAGAGCGG
GCGGGTGAGT
CACCCACACA
AAGGAAAAGG
GCCTTTCCGT
CCTCAGCCGT
CGCTTCATGT
GACTCCACGG
AGTACCGGGC
GCCGTCCAGG
CACCTCGATT
AGTTCTCGAG
CTTTTGGAGT
ACGTCGTCTT
TAGGTTGGGG
GGAGGGGTTT
TATGCGATGG
AGTTTCCCCA
CACTGAGTGG
GTGGAGACTG
AAGTTAGGCC
AGCTTGGCAC
TTGATGTAAT
TCTCCTTGGA
ATTTGCCCTT
TTTGAGTTTG
GATCTTGGTT
CATTCTCAAG
CCTCAGACAG
TGGTTCAAAG
TTTTTTTCTT
CCATTTCAGG
TGTCGTGAGG
AATTGGGATC
CCCGGACGCC
ACCATGTACC
CATACGACGT
CCCAGACTAC
GCTGAATTCA
TGCCCCACTC
CAGCCTGCAT
CCATCCATCC
CGTGTCCCAG
GGGTCACGGG
GCCCAGAAGG
CAGCCTTGGT
TCTGCTGAGT
GCCTGCCTGG
TGACCCTTTG
GGGGCTAGGA
GAGCCACCAG
AGCACACTCT
CCGGTACCTG
GTGCTCCACC
TAGCCTCCCT
GCAGCTGGGA
CTGCTGTTAA
ACGGGGTCTG
CAGCCTGGCT
GAGGAGCTGC
GCCACATCCA
CTCCAGGTAC
CGGGGCAGCT
ACTGGAGGAC
TGTGCGGGCC
TGCCTGGGCT
GCCCCCTCCG
CCGTGGGGCC
CTGTTGCTGC
TGTCCATCTA
TTTCTACTAC
TCCCTCCCAA
ATGCGGTCGG
CCCGCCCTTC
ACTTGGATGC
TTGCCCTCCT
GGGCCTCTCG
CAGGCACTGA
ACATCCTCCT
GGGCCTCAAG
GGCCTGGCCC
CAGCTGAGAT
CTCTGCAGTG
TGTGAAAAAG
GGAATTTCAA
CGTGGCCCAT
GGGCTGGCAT
GGTCATATTA
CATCGGATAT
CTGCGGCTGA
TCCTGCCAGA
GCTCCAGGCC
CGGATTCGAA
CTTACAATCA
GCATTACAAC
AACCTGCTAC
GGGGTGCAGT
GAGCCAGCGG
CTGTATATTC
TCCTCCCATT
GGACTGTGGG
GTGCCTGATA
ACCTGAGTAT
GGCTGACCCC
AACATTCGCT
TCCTGGATAA
ACTGCCCCAG
CAGACCGGTG
ACCATGCTGG
CATCAAGGAT
CGGGTTTACA
GCAACAGCAT
CTATGAGCTT
CTGGAGAACG
GGCAGCGGGC
GGGCACCTGT
GTCCTGGAGT
ACGCCACCCC
CTTGCAGACT
TTGTTTGCCA
TGTCACAATA
CAGTCAAGCT
GGCTTTAGCC
GGGAGGATAG
GCTTGAGCAG
GCCAAACTCT
TCTGCCGGAC
ACTTGAGGAC
ATCCTGGCAG
ATGCCCCTGA
GTCTCAGAAC
AACTGCCGCC
TCATTGCCTA
CCAGGAACCT
GCAGATGACA
GCAGCTTCTC
GCTGTCCCAG
GAGGTTCTCC
GGCACCTGCG
GCAGGAGGAA
AAGGAAGAGG
TTACTGTGGG
CAGCTTGAAG
ACCTCAGCGG
TGCCCAGTAC
CTCCACGATG
TCCCAAGAGC
CTGAGCTCCT
CATCAGTGGA
ATGGAAAAGC
CCCTCCCTCT
CCGCACGGAT
TTCTCTTGAA
CTAGTGATCC
GCATGCATCT
AGGGCGGCCA
ATTCCGCCCC
TCTCCCCCCC
CCCCCTCTCC
CTCCCCCCCC
CCTAACGTTA
CTGGCCGAAG
CCGCTTGGAA
TAAGGCCGGT
GTGCGTTTGT
CTATATGTTA
TTTTCCACCA
TATTGCCGTC
TTTTGGCAAT
GTGAGGGCCC
GGAAACCTGG
CCCTGTCTTC
TTGACGAGCA
TTCCTAGGGG
TCTTTCCCCT
CTCGCCAAAG
GAATGCAAGG
TCTGTTGAAT
GTCGTGAAGG
AAGCAGTTCC
TCTGGAAGCT
TCTTGAAGAC
AAACAACGTC
TGTAGCGACC
CTTTGCAGGC
AGCGGAACCC
CCCACCTGGC
GACAGGTGCC
TCTGCGGCCA
AAAGCCACGT
GTATAAGATA
CACCTGCAAA
GGCGGCACAA
CCCCAGTGCC
ACGTTGTGAG
TTGGATAGTT
GTGGAAAGAG
TCAAATGGCT
CTCCTCAAGC
GTATTCAACA
AGGGGCTGAA
GGATGCCCAG
AAGGTACCCC
ATTGTATGGG
ATCTGATCTG
GGGCCTCGGT
GCACATGCTT
TACATGTGTT
TAGTCGAGGT
TAAAAAAACG
TCTAGGCCCC
CCGAACCACG
GGGACGTGGT
TTTCCTTTGA
AAAACACGAT
GATAAGCTTG
CCACAACCCA
CAAGGAGACG
ACCTTCCATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCCG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
CCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGCGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTGATAA
TAGGCGGCCG
CTCGAGACCT
AGAAAAACAT
GGAGCAATCA
CAAGTAGCAA
TACAGCAGCT
ACCAATGCTG
ATTGTGCCTG
GCTAGAAGCA
CAAGAGGAGG
AGGAGGTGGG
TTTTCCAGTC
ACACCTCAGG
TACCTTTAAG
ACCAATGACT
TACAAGGCAG
CTGTAGATCT
TAGCCACTTT
TTAAAAGAAA
AGGGGGGACT
GGAAGGGCTA
ATTCACTCCC
AACGAAGACA
AGATCTGCTT
TTTGCTTGTA
CTGGGTCTCT
CTGGTTAGAC
CAGATCTGAG
CCTGGGAGCT
CTCTGGCTAA
CTAGGGAACC
CACTGCTTAA
GCCTCAATAA
AGCTTGCCTT
GAGTGCTTCA
AGTAGTGTGT
GCCCGTCTGT
TGTGTGACTC
TGGTAACTAG
AGATCCCTCA
GACCCTTTTA
GTCAGTGTGG
AAAATCTCTA
GCAGTAGTAG
TTCATGTCAT
CTTATTATTC
AGTATTTATA
ACTTGCAAAG
AAATGAATAT
CAGAGAGTGA
GAGGCCTTGA
CATTATAATA
GATTTAGCAG
GAATTGAACT
AGGAGTGGAG
CACACAGGCA
AAGTTCTAGA
GCTCGCTGAT
CAGCCTCGAC
TGTGCCTTCT
AGTTGCCAGC
CATCTGTTGT
TTGCCCCTCC
CCCGTGCCTT
CCTTGACCCT
GGAAGGTGCC
ACTCCCACTG
TCCTTTCCTA
ATAAAATGAG
GAAATTGCAT
CGCATTGTCT
GAGTAGGTGT
CATTCTATTC
TGGGGGGTGG
GGGTGGGGCA
GGACAGCAAG
GGGGGAGGAT
TGGGAAGACA
ATAGCAGGCA
TGCTGGGGAT
GCGGTGGGCT
CTATGGCTTC
TGAGGCGGAA
AGAACCAGCT
GGGGGCGCGC
CCCTCGAGGC
CGCCATGGTC
ATAGCTGTTT
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCACGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTTCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCTA
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCTGGCC
TTTTGCA