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pSIN-EF2-Steep1(mouse)-HA-Puro

产品货号:P70869

产品规格:

    少量干粉质粒 甘油菌种 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pSIN-EF2-Steep1(mouse)-HA-Puro
别称: NM_029951.1,Steep1/C330007P06Rik/STEEP
基因长: 666bp
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 蛋白表达
片段类型: ORF
片段物种: 小鼠
原核抗性: Amp
筛选标记: Puro
荧光标记:
启动子: EF1a
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体: pSIN-EF2-puro
正向引物: PEF-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。

二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CGGACCGCCA

CTGCCAATTA

CCTGTGGTTT

CATTTACTCT

AAACCTGTGA

TTCCTCTGAA

61

TTATTTTCAT

TTTAAAGAAA

TTGTATTTGT

TAAATATGTA

CTACAAACTT

AGTAGTTGGA

121

AGGGCTAATT

CACTCCCAAA

GAAGACAAGA

TATCCTTGAT

CTGTGGATCT

ACCACACACA

181

AGGCTACTTC

CCTGATTAGC

AGAACTACAC

ACCAGGGCCA

GGGGTCAGAT

ATCCACTGAC

241

CTTTGGATGG

TGCTACAAGC

TAGTACCAGT

TGAGCCAGAT

AAGGTAGAAG

AGGCCAATAA

301

AGGAGAGAAC

ACCAGCTTGT

TACACCCTGT

GAGCCTGCAT

GGGATGGATG

ACCCGGAGAG

361

AGAAGTGTTA

GAGTGGAGGT

TTGACAGCCG

CCTAGCATTT

CATCACGTGG

CCCGAGAGCT

421

GCATCCGGAG

TACTTCAAGA

ACTGCTGATA

TCGAGCTTGC

TACAAGGGAC

TTTCCGCTGG

481

GGACTTTCCA

GGGAGGCGTG

GCCTGGGCGG

GACTGGGGAG

TGGCGAGCCC

TCAGATCCTG

541

CATATAAGCA

GCTGCTTTTT

GCCTGTACTG

GGTCTCTCTG

GTTAGACCAG

ATCTGAGCCT

601

GGGAGCTCTC

TGGCTAACTA

GGGAACCCAC

TGCTTAAGCC

TCAATAAAGC

TTGCCTTGAG

661

TGCTTCAAGT

AGTGTGTGCC

CGTCTGTTGT

GTGACTCTGG

TAACTAGAGA

TCCCTCAGAC

721

CCTTTTAGTC

AGTGTGGAAA

ATCTCTAGCA

GTGGCGCCCG

AACAGGGACT

TGAAAGCGAA

781

AGGGAAACCA

GAGGAGCTCT

CTCGACGCAG

GACTCGGCTT

GCTGAAGCGC

GCACGGCAAG

841

AGGCGAGGGG

CGGCGACTGG

TGAGTACGCC

AAAAATTTTG

ACTAGCGGAG

GCTAGAAGGA

901

GAGAGATGGG

TGCGAGAGCG

TCAGTATTAA

GCGGGGGAGA

ATTAGATCGC

GATGGGAAAA

961

AATTCGGTTA

AGGCCAGGGG

GAAAGAAAAA

ATATAAATTA

AAACATATAG

TATGGGCAAG

1021

CAGGGAGCTA

GAACGATTCG

CAGTTAATCC

TGGCCTGTTA

GAAACATCAG

AAGGCTGTAG

1081

ACAAATACTG

GGACAGCTAC

AACCATCCCT

TCAGACAGGA

TCAGAAGAAC

TTAGATCATT

1141

ATATAATACA

GTAGCAACCC

TCTATTGTGT

GCATCAAAGG

ATAGAGATAA

AAGACACCAA

1201

GGAAGCTTTA

GACAAGATAG

AGGAAGAGCA

AAACAAAAGT

AAGACCACCG

CACAGCAAGC

1261

GGCCGCTGAT

CTTCAGACCT

GGAGGAGGAG

ATATGAGGGA

CAATTGGAGA

AGTGAATTAT

1321

ATAAATATAA

AGTAGTAAAA

ATTGAACCAT

TAGGAGTAGC

ACCCACCAAG

GCAAAGAGAA

1381

GAGTGGTGCA

GAGAGAAAAA

AGAGCAGTGG

GAATAGGAGC

TTTGTTCCTT

GGGTTCTTGG

1441

GAGCAGCAGG

AAGCACTATG

GGCGCAGCGT

CAATGACGCT

GACGGTACAG

GCCAGACAAT

1501

TATTGTCTGG

TATAGTGCAG

CAGCAGAACA

ATTTGCTGAG

GGCTATTGAG

GCGCAACAGC

1561

ATCTGTTGCA

ACTCACAGTC

TGGGGCATCA

AGCAGCTCCA

GGCAAGAATC

CTGGCTGTGG

1621

AAAGATACCT

AAAGGATCAA

CAGCTCCTGG

GGATTTGGGG

TTGCTCTGGA

AAACTCATTT

1681

GCACCACTGC

TGTGCCTTGG

AATGCTAGTT

GGAGTAATAA

ATCTCTGGAA

CAGATTTGGA

1741

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

TTACACAAGC

TTAATACACT

1801

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

ACAAGAATTA

TTGGAATTAG

1861

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

TTGGCTGTGG

TATATAAAAT

1921

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

AGTTTTTGCT

GTACTTTCTA

1981

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

TCAGACCCAC

CTCCCAACCC

2041

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

TGGAGAGAGA

GACAGAGACA

2101

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGCCA

CAAATGGCAG

TATTCATCCA

2161

CAATTTTAAA

AGAAAGGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

2221

AATAGCAACA

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAA

TTCAAAATTT

2281

TCGGGTTTAT

TACAGGGACA

GCAGAGATCC

ACTTTGGATC

GATAAGCTTT

GCAAAGATGG

2341

ATAAAGTTTT

AAACAGAGAG

GAATCTTTGC

AGCTAATGGA

CCTTCTAGGT

CTTGAAAGGA

2401

GTGGGAATTG

GCTCCGGTGC

CCGTCAGTGG

GCAGAGCGCA

CATCGCCCAC

AGTCCCCGAG

2461

AAGTTGGGGG

GAGGGGTCGG

CAATTGAACC

GGTGCCTAGA

GAAGGTGGCG

CGGGGTAAAC

2521

TGGGAAAGTG

ATGTCGTGTA

CTGGCTCCGC

CTTTTTCCCG

AGGGTGGGGG

AGAACCGTAT

2581

ATAAGTGCAG

TAGTCGCCGT

GAACGTTCTT

TTTCGCAACG

GGTTTGCCGC

CAGAACACAG

2641

GTAAGTGCCG

TGTGTGGTTC

CCGCGGGCCT

GGCCTCTTTA

CGGGTTATGG

CCCTTGCGTG

2701

CCTTGAATTA

CTTCCACCTG

GCTGCAGTAC

GTGATTCTTG

ATCCCGAGCT

TCGGGTTGGA

2761

AGTGGGTGGG

AGAGTTCGAG

GCCTTGCGCT

TAAGGAGCCC

CTTCGCCTCG

TGCTTGAGTT

2821

GAGGCCTGGC

CTGGGCGCTG

GGGCCGCCGC

GTGCGAATCT

GGTGGCACCT

TCGCGCCTGT

2881

CTCGCTGCTT

TCGATAAGTC

TCTAGCCATT

TAAAATTTTT

GATGACCTGC

TGCGACGCTT

2941

TTTTTCTGGC

AAGATAGTCT

TGTAAATGCG

GGCCAAGATC

TGCACACTGG

TATTTCGGTT

3001

TTTGGGGCCG

CGGGCGGCGA

CGGGGCCCGT

GCGTCCCAGC

GCACATGTTC

GGCGAGGCGG

3061

GGCCTGCGAG

CGCGGCCACC

GAGAATCGGA

CGGGGGTAGT

CTCAAGCTGG

CCGGCCTGCT

3121

CTGGTGCCTG

GCCTCGCGCC

GCCGTGTATC

GCCCCGCCCT

GGGCGGCAAG

GCTGGCCCGG

3181

TCGGCACCAG

TTGCGTGAGC

GGAAAGATGG

CCGCTTCCCG

GCCCTGCTGC

AGGGAGCTCA

3241

AAATGGAGGA

CGCGGCGCTC

GGGAGAGCGG

GCGGGTGAGT

CACCCACACA

AAGGAAAAGG

3301

GCCTTTCCGT

CCTCAGCCGT

CGCTTCATGT

GACTCCACGG

AGTACCGGGC

GCCGTCCAGG

3361

CACCTCGATT

AGTTCTCGAG

CTTTTGGAGT

ACGTCGTCTT

TAGGTTGGGG

GGAGGGGTTT

3421

TATGCGATGG

AGTTTCCCCA

CACTGAGTGG

GTGGAGACTG

AAGTTAGGCC

AGCTTGGCAC

3481

TTGATGTAAT

TCTCCTTGGA

ATTTGCCCTT

TTTGAGTTTG

GATCTTGGTT

CATTCTCAAG

3541

CCTCAGACAG

TGGTTCAAAG

TTTTTTTCTT

CCATTTCAGG

TGTCGTGAGG

AATTGGGATC

3601

CGCCACCATG

CCGAAAGTCG

TGTCGCGGTC

GGTGGTCTGC

TCAGACACCC

GGGACCGCGA

3661

GGAATATGAT

GATGGCGAGA

AGCCCCTCCA

TGTCTACTAC

TGTTTATGTG

GCCAGATGGT

3721

CCTGGTGCTG

GATTGCCAGT

TAGAGAAATT

GCCCATGAGG

CCCCGGGACC

GGTCTCGCGT

3781

GATCGATGCT

GCCAAACATG

CTCACAAGTT

TTGTAACACA

GAAGATGAGG

AGACTACTTA

3841

TCTTCGGAGG

CCTGAGGGCA

TTGAACGTCA

GTACAGGAAG

AAGTGTGCAA

AGTGTGGACT

3901

GCCGCTCTTC

TACCAATCCC

AGCCGAAGAA

TGCTCCTGTG

ACCTTCATTG

TGGATGGAGC

3961

AGTAGTGAAA

TTTGGCCAAG

GCTTTGGGAA

AACCAATATA

TATACTCAGA

AACAAGAGCC

4021

TCCTAAGAAG

GTTATGATGA

CCAAAAGGAC

TAAAGACATG

GGCAAGTTCA

GCTCTGTTAC

4081

TGTGTCTACC

ATTGATGAAG

AAGAAGAGGA

GATAGAGGCT

AGGGAAGTTG

CTGACTCTTA

4141

CGCGCAGAAT

GCCAAAGTGA

TTGAAAAGCA

GCTGGAGCGC

AAAGGCATGA

GCAAGAGGAG

4201

GCTGCAGGAG

TTGGCTGAAT

TGGAAGCCAA

GAAAGCAAAA

ATGAAGGGGA

CCCTGATCGA

4261

CAATCAGTTC

AAATACCCAT

ACGACGTACC

AGATTACGCT

TGAGAATTCT

TCGAACATAT

4321

GAAGCTTGCT

AGCACTAGTG

ATCCGCATGC

ATCTAGGGCG

GCCAATTCCG

CCCCTCTCCC

4381

CCCCCCCCCT

CTCCCTCCCC

CCCCCCTAAC

GTTACTGGCC

GAAGCCGCTT

GGAATAAGGC

4441

CGGTGTGCGT

TTGTCTATAT

GTTATTTTCC

ACCATATTGC

CGTCTTTTGG

CAATGTGAGG

4501

GCCCGGAAAC

CTGGCCCTGT

CTTCTTGACG

AGCATTCCTA

GGGGTCTTTC

CCCTCTCGCC

4561

AAAGGAATGC

AAGGTCTGTT

GAATGTCGTG

AAGGAAGCAG

TTCCTCTGGA

AGCTTCTTGA

4621

AGACAAACAA

CGTCTGTAGC

GACCCTTTGC

AGGCAGCGGA

ACCCCCCACC

TGGCGACAGG

4681

TGCCTCTGCG

GCCAAAAGCC

ACGTGTATAA

GATACACCTG

CAAAGGCGGC

ACAACCCCAG

4741

TGCCACGTTG

TGAGTTGGAT

AGTTGTGGAA

AGAGTCAAAT

GGCTCTCCTC

AAGCGTATTC

4801

AACAAGGGGC

TGAAGGATGC

CCAGAAGGTA

CCCCATTGTA

TGGGATCTGA

TCTGGGGCCT

4861

CGGTGCACAT

GCTTTACATG

TGTTTAGTCG

AGGTTAAAAA

AACGTCTAGG

CCCCCCGAAC

4921

CACGGGGACG

TGGTTTTCCT

TTGAAAAACA

CGATGATAAG

CTTGCCACAA

CCCACAAGGA

4981

GACGACCTTC

CATGACCGAG

TACAAGCCCA

CGGTGCGCCT

CGCCACCCGC

GACGACGTCC

5041

CCCGGGCCGT

ACGCACCCTC

GCCGCCGCGT

TCGCCGACTA

CCCCGCCACG

CGCCACACCG

5101

TCGACCCGGA

CCGCCACATC

GAGCGGGTCA

CCGAGCTGCA

AGAACTCTTC

CTCACGCGCG

5161

TCGGGCTCGA

CATCGGCAAG

GTGTGGGTCG

CGGACGACGG

CGCCGCGGTG

GCGGTCTGGA

5221

CCACGCCGGA

GAGCGTCGAA

GCGGGGGCGG

TGTTCGCCGA

GATCGGCCCG

CGCATGGCCG

5281

AGTTGAGCGG

TTCCCGGCTG

GCCGCGCAGC

AACAGATGGA

AGGCCTCCTG

GCGCCGCACC

5341

GGCCCAAGGA

GCCCGCGTGG

TTCCTGGCCA

CCGTCGGCGT

CTCGCCCGAC

CACCAGGGCA

5401

AGGGTCTGGG

CAGCGCCGTC

GTGCTCCCCG

GAGTGGAGGC

GGCCGAGCGC

GCCGGGGTGC

5461

CCGCCTTCCT

GGAGACCTCC

GCGCCCCGCA

ACCTCCCCTT

CTACGAGCGG

CTCGGCTTCA

5521

CCGTCACCGC

CGACGTCGAG

GTGCCCGAAG

GACCGCGCAC

CTGGTGCATG

ACCCGCAAGC

5581

CCGGTGCCTG

ATAATAGGCG

GCCGCTCGAG

ACCTAGAAAA

ACATGGAGCA

ATCACAAGTA

5641

GCAATACAGC

AGCTACCAAT

GCTGATTGTG

CCTGGCTAGA

AGCACAAGAG

GAGGAGGAGG

5701

TGGGTTTTCC

AGTCACACCT

CAGGTACCTT

TAAGACCAAT

GACTTACAAG

GCAGCTGTAG

5761

ATCTTAGCCA

CTTTTTAAAA

GAAAAGGGGG

GACTGGAAGG

GCTAATTCAC

TCCCAACGAA

5821

GACAAGATCT

GCTTTTTGCT

TGTACTGGGT

CTCTCTGGTT

AGACCAGATC

TGAGCCTGGG

5881

AGCTCTCTGG

CTAACTAGGG

AACCCACTGC

TTAAGCCTCA

ATAAAGCTTG

CCTTGAGTGC

5941

TTCAAGTAGT

GTGTGCCCGT

CTGTTGTGTG

ACTCTGGTAA

CTAGAGATCC

CTCAGACCCT

6001

TTTAGTCAGT

GTGGAAAATC

TCTAGCAGTA

GTAGTTCATG

TCATCTTATT

ATTCAGTATT

6061

TATAACTTGC

AAAGAAATGA

ATATCAGAGA

GTGAGAGGCC

TTGACATTAT

AATAGATTTA

6121

GCAGGAATTG

AACTAGGAGT

GGAGCACACA

GGCAAAGTTC

TAGAGCTCGC

TGATCAGCCT

6181

CGACTGTGCC

TTCTAGTTGC

CAGCCATCTG

TTGTTTGCCC

CTCCCCCGTG

CCTTCCTTGA

6241

CCCTGGAAGG

TGCCACTCCC

ACTGTCCTTT

CCTAATAAAA

TGAGGAAATT

GCATCGCATT

6301

GTCTGAGTAG

GTGTCATTCT

ATTCTGGGGG

GTGGGGGTGG

GGCAGGACAG

CAAGGGGGGA

6361

GGATTGGGAA

GACAATAGCA

GGCATGCTGG

GGATGCGGTG

GGCTCTATGG

CTTCTGAGGC

6421

GGAAAGAACC

AGCTGGGGGC

GCGCCCCTCG

AGGCCGCCAT

GGTCATAGCT

GTTTGACGTC

6481

AGGTGGCACT

TTTCGGGGAA

ATGTGCGCGG

AACCCCTATT

TGTTTATTTT

TCTAAATACA

6541

TTCAAATATG

TATCCGCTCA

CGAGACAATA

ACCCTGATAA

ATGCTTCAAT

AATATTGAAA

6601

AAGGAAGAGT

ATGAGTATTC

AACATTTCCG

TGTCGCCCTT

ATTCCCTTTT

TTGCGGCATT

6661

TTGCCTTCCT

GTTTTTGCTC

ACCCAGAAAC

GCTGGTGAAA

GTAAAAGATG

CTGAAGATCA

6721

GTTGGGTGCA

CGAGTGGGTT

ACATCGAACT

GGATCTCAAC

AGCGGTAAGA

TCCTTGAGAG

6781

TTTTCGCCCC

GAAGAACGTT

TTCCAATGAT

GAGCACTTTT

AAAGTTCTGC

TATGTGGCGC

6841

GGTATTATCC

CGTATTGACG

CCGGGCAAGA

GCAACTCGGT

CGCCGCATAC

ACTATTCTCA

6901

GAATGACTTG

GTTGAGTACT

CACCAGTCAC

AGAAAAGCAT

CTTACGGATG

GCATGACAGT

6961

AAGAGAATTA

TGCAGTGCTG

CCATAACCAT

GAGTGATAAC

ACTGCGGCCA

ACTTACTTCT

7021

GACAACGATC

GGAGGACCGA

AGGAGCTAAC

CGCTTTTTTG

CACAACATGG

GGGATCATGT

7081

AACTCGCCTT

GATCGTTGGG

AACCGGAGCT

GAATGAAGCC

ATACCAAACG

ACGAGCGTGA

7141

CACCACGATG

CCTGTAGCAA

TGGCAACAAC

GTTGCGCAAA

CTATTAACTG

GCGAACTACT

7201

TACTCTAGCT

TCCCGGCAAC

AATTAATAGA

CTGGATGGAG

GCGGATAAAG

TTGCAGGACC

7261

ACTTCTGCGC

TCGGCCCTTC

CGGCTGGCTG

GTTTATTGCT

GATAAATCTG

GAGCCGGTGA

7321

GCGTGGGTCT

CGCGGTATCA

TTGCAGCACT

GGGGCCAGAT

GGTAAGCCCT

CCCGTATCGT

7381

AGTTATCTAC

ACGACGGGGA

GTCAGGCAAC

TATGGATGAA

CGAAATAGAC

AGATCGCTGA

7441

GATAGGTGCC

TCACTGATTA

AGCATTGGTA

ACTGTCAGAC

CAAGTTTACT

CATATATACT

7501

TTAGATTGAT

TTAAAACTTC

ATTTTTAATT

TAAAAGGATC

TAGGTGAAGA

TCCTTTTTGA

7561

TAATCTCATG

ACCAAAATCC

CTTAACGTGA

GTTTTCGTTC

CACTGAGCGT

CAGACCCCGT

7621

AGAAAAGATC

AAAGGATCTT

CTTGAGATCC

TTTTTTTCTG

CGCGTAATCT

GCTGCTTGCA

7681

AACAAAAAAA

CCACCGCTAC

CAGCGGTGGT

TTGTTTGCCG

GATCAAGAGC

TACCAACTCT

7741

TTTTCCGAAG

GTAACTGGCT

TCAGCAGAGC

GCAGATACCA

AATACTGTTC

TTCTAGTGTA

7801

GCCGTAGTTA

GGCCACCACT

TCAAGAACTC

TGTAGCACCG

CCTACATACC

TCGCTCTGCT

7861

AATCCTGTTA

CCAGTGGCTG

CTGCCAGTGG

CGATAAGTCG

TGTCTTACCG

GGTTGGACTC

7921

AAGACGATAG

TTACCGGATA

AGCGCAGCGG

TCGGGCTGAA

CGGGGGGTTC

GTGCACACAG

7981

CCCAGCTTGG

AGCGAACGAC

CTACACCGAA

CTGAGATACC

TACAGCGTGA

GCTATGAGAA

8041

AGCGCCACGC

TTCCCGAAGG

GAGAAAGGCG

GACAGGTATC

CGGTAAGCGG

CAGGGTCGGA

8101

ACAGGAGAGC

GCACGAGGGA

GCTTCCAGGG

GGAAACGCCT

GGTATCTTTA

TAGTCCTGTC

8161

GGGTTTCGCC

ACCTCTGACT

TGAGCGTCGA

TTTTTGTGAT

GCTCGTCAGG

GGGGCGGAGC

8221

CTATGGAAAA

ACGCCAGCAA

CGCGGCCTTT

TTACGGTTCT

GGCCTTTTGC

A

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