名称: | pLV2-CMV-PDHA1(human)-EBFP |
---|---|
别称: | NM_000284.4;E1alpha; PDHA; PDHAD; PDHCE1A; PHE1A |
基因长: | 1170bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 蓝色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV2-CMV-CTSB(human)-mCherry |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGACTC
TACTAGAGGA
TCGCTAGCGC
TACCGGACTC
AGATCTCGAG
GCCACCATGA
GGAAGATGCT
CGCCGCCGTC
TCCCGCGTGC
TGTCTGGCGC
TTCTCAGAAG
CCGGCAAGCA
GAGTGCTGGT
AGCATCCCGT
AATTTTGCAA
ATGATGCTAC
ATTTGAAATT
AAGAAATGTG
ACCTTCACCG
GCTGGAAGAA
GGCCCTCCTG
TCACAACAGT
GCTCACCAGG
GAGGATGGGC
TCAAATACTA
CAGGATGATG
CAGACTGTAC
GCCGAATGGA
GTTGAAAGCA
GATCAGCTGT
ATAAACAGAA
AATTATTCGT
GGTTTCTGTC
ACTTGTGTGA
TGGTCAGGAA
GCTTGCTGTG
TGGGCCTGGA
GGCCGGCATC
AACCCCACAG
ACCATCTCAT
CACAGCCTAC
CGGGCTCACG
GCTTTACTTT
CACCCGGGGC
CTTTCCGTCC
GAGAAATTCT
CGCAGAGCTT
ACAGGACGAA
AAGGAGGTTG
TGCTAAAGGG
AAAGGAGGAT
CGATGCACAT
GTATGCCAAG
AACTTCTACG
GGGGCAATGG
CATCGTGGGA
GCGCAGGTGC
CCCTGGGCGC
TGGGATTGCT
CTAGCCTGTA
AGTATAATGG
AAAAGATGAG
GTCTGCCTGA
CTTTATATGG
CGATGGTGCT
GCTAACCAGG
GCCAGATATT
CGAAGCTTAC
AACATGGCAG
CTTTGTGGAA
ATTACCTTGT
ATTTTCATCT
GTGAGAATAA
TCGCTATGGA
ATGGGAACGT
CTGTTGAGAG
AGCGGCAGCC
AGCACTGATT
ACTACAAGAG
AGGCGATTTC
ATTCCTGGGC
TGAGAGTGGA
TGGAATGGAT
ATCCTGTGCG
TCCGAGAGGC
AACAAGGTTT
GCTGCTGCCT
ATTGTAGATC
TGGGAAGGGG
CCCATCCTGA
TGGAGCTGCA
GACTTACCGT
TACCACGGAC
ACAGTATGAG
TGACCCTGGA
GTCAGTTACC
GTACACGAGA
AGAAATTCAG
GAAGTAAGAA
GTAAGAGTGA
CCCTATTATG
CTTCTCAAGG
ACAGGATGGT
GAACAGCAAT
CTTGCCAGTG
TGGAAGAACT
AAAGGAAATT
GATGTGGAAG
TGAGGAAGGA
GATTGAGGAT
GCTGCCCAGT
TTGCCACGGC
CGATCCTGAG
CCACCTTTGG
AAGAGCTGGG
CTACCACATC
TACTCCAGCG
ACCCACCTTT
TGAAGTTCGT
GGTGCCAATC
AGTGGATCAA
GTTTAAGTCA
GTCAGTGGCG
GTGGAGGAAG
CGGCGGAGGT
GGATCTCGGG
ATCCACCGGT
CATGGTGAGC
AAGGGCGAGG
AGCTGTTCAC
CGGGGTGGTG
CCCATCCTGG
TCGAGCTGGA
CGGCGACGTA
AACGGCCACA
AGTTCAGCGT
GTCCGGCGAG
GGCGAGGGCG
ATGCCACCTA
CGGCAAGCTG
ACCCTGAAGT
TCATCTGCAC
CACCGGCAAG
CTGCCCGTGC
CCTGGCCCAC
CCTCGTGACC
ACCCTGACCC
ACGGCGTGCA
GTGCTTCAGC
CGCTACCCCG
ACCACATGAA
GCAGCACGAC
TTCTTCAAGT
CCGCCATGCC
CGAAGGCTAC
GTCCAGGAGC
GCACCATCTT
CTTCAAGGAC
GACGGCAACT
ACAAGACCCG
CGCCGAGGTG
AAGTTCGAGG
GCGACACCCT
GGTGAACCGC
ATCGAGCTGA
AGGGCATCGA
CTTCAAGGAG
GACGGCAACA
TCCTGGGGCA
CAAGCTGGAG
TACAACTTCA
ACAGCCACAA
CGTCTATATC
ATGGCCGACA
AGCAGAAGAA
CGGCATCAAG
GTGAACTTCA
AGATCCGCCA
CAACATCGAG
GACGGCAGCG
TGCAGCTCGC
CGACCACTAC
CAGCAGAACA
CCCCCATCGG
CGACGGCCCC
GTGCTGCTGC
CCGACAACCA
CTACCTGAGC
ACCCAGTCCG
CCCTGAGCAA
AGACCCCAAC
GAGAAGCGCG
ATCACATGGT
CCTGCTGGAG
TTCGTGACCG
CCGCCGGGAT
CACTCTCGGC
ATGGACGAGC
TGTACAAGTA
GCGGCCGCGA
CTCTAGATAA
TTCTACCCCG
CGTCTGGAAC
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAGCTGA
CGTCCTTTCC
ATGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGGAATTAAT
TCTGCAGTCG
AGACCTAGAA
AAACATGGAG
CAATCACAAG
TAGCAATACA
GCAGCTACCA
ATGCTGATTG
TGCCTGGCTA
GAAGCACAAG
AGGAGGAGGA
GGTGGGTTTT
CCAGTCACAC
CTCAGGTACC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGAG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGACAAGAT
ATCCTTGATC
TGTGGATCTA
CCACACACAA
GGCTACTTCC
CTGATTAGCA
GAACTACACA
CCAGGGCCAG
GGGTCAGATA
TCCACTGACC
TTTGGATGGT
GCTACAAGCT
AGTACCAGTT
GAGCCAGATA
AGGTAGAAGA
GGCCAATAAA
GGAGAGAACA
CCAGCTTGTT
ACACCCTGTG
AGCCTGCATG
GGATGGATGA
CCCGGAGAGA
GAAGTGTTAG
AGTGGAGGTT
TGACAGCCGC
CTAGCATTTC
ATCACGTGGC
CCGAGAGCTG
CATCCGGAGT
ACTTCAAGAA
CTGCTGATAT
CGAGCTTGCT
ACAAGGGACT
TTCCGCTGGG
GACTTTCCAG
GGAGGCGTGG
CCTGGGCGGG
ACTGGGGAGT
GGCGAGCCCT
CAGATCCTGC
ATATAAGCAG
CTGCTTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TAGTAGTTCA
TGTCATCTTA
TTATTCAGTA
TTTATAACTT
GCAAAGAAAT
GAATATCAGA
GAGTGAGAGG
CCTTGACATT
GCTAGCGTTT
ACCGTCGACC
TCTAGCTAGA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCGACGGA
TCGGGAGATC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGATCAACTG
GATAACTCAA
GCTAACCAAA
ATCATCCCAA
ACTTCCCACC
CCATACCCTA
TTACCACTGC
CAATTACCTG
TGGTTTCATT
TACTCTAAAC
CTGTGATTCC
TCTGAATTAT
TTTCATTTTA
AAGAAATTGT
ATTTGTTAAA
TATGTACTAC
AAACTTAGTA
GTTGGAAGGG
CTAATTCACT
CCCAAAGAAG
ACAAGATATC
CTTGATCTGT
GGATCTACCA
CACACAAGGC
TACTTCCCTG
ATTAGCAGAA
CTACACACCA
GGGCCAGGGG
TCAGATATCC
ACTGACCTTT
GGATGGTGCT
ACAAGCTAGT
ACCAGTTGAG
CCAGATAAGG
TAGAAGAGGC
CAATAAAGGA
GAGAACACCA
GCTTGTTACA
CCCTGTGAGC
CTGCATGGGA
TGGATGACCC
GGAGAGAGAA
GTGTTAGAGT
GGAGGTTTGA
CAGCCGCCTA
GCATTTCATC
ACGTGGCCCG
AGAGCTGCAT
CCGGAGTACT
TCAAGAACTG
CTGATATCGA
GCTTGCTACA
AGGGACTTTC
CGCTGGGGAC
TTTCCAGGGA
GGCGTGGCCT
GGGCGGGACT
GGGGAGTGGC
GAGCCCTCAG
ATCCTGCATA
TAAGCAGCTG
CTTTTTGCCT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTGG
CGCCCGAACA
GGGACTTGAA
AGCGAAAGGG
AAACCAGAGG
AGCTCTCTCG
ACGCAGGACT
CGGCTTGCTG
AAGCGCGCAC
GGCAAGAGGC
GAGGGGCGGC
GACTGGTGAG
TACGCCAAAA
ATTTTGACTA
GCGGAGGCTA
GAAGGAGAGA
GATGGGTGCG
AGAGCGTCAG
TATTAAGCGG
GGGAGAATTA
GATCGCGATG
GGAAAAAATT
CGGTTAAGGC
CAGGGGGAAA
GAAAAAATAT
AAATTAAAAC
ATATAGTATG
GGCAAGCAGG
GAGCTAGAAC
GATTCGCAGT
TAATCCTGGC
CTGTTAGAAA
CATCAGAAGG
CTGTAGACAA
ATACTGGGAC
AGCTACAACC
ATCCCTTCAG
ACAGGATCAG
AAGAACTTAG
ATCATTATAT
AATACAGTAG
CAACCCTCTA
TTGTGTGCAT
CAAAGGATAG
AGATAAAAGA
CACCAAGGAA
GCTTTAGACA
AGATAGAGGA
AGAGCAAAAC
AAAAGTAAGA
CCACCGCACA
GCAAGCGGCC
GGCCGCTGAT
CTTCAGACCT
GGAGGAGGAG
ATATGAGGGA
CAATTGGAGA
AGTGAATTAT
ATAAATATAA
AGTAGTAAAA
ATTGAACCAT
TAGGAGTAGC
ACCCACCAAG
GCAAAGAGAA
GAGTGGTGCA
GAGAGAAAAA
AGAGCAGTGG
GAATAGGAGC
TTTGTTCCTT
GGGTTCTTGG
GAGCAGCAGG
AAGCACTATG
GGCGCAGCGT
CAATGACGCT
GACGGTACAG
GCCAGACAAT
TATTGTCTGG
TATAGTGCAG
CAGCAGAACA
ATTTGCTGAG
GGCTATTGAG
GCGCAACAGC
ATCTGTTGCA
ACTCACAGTC
TGGGGCATCA
AGCAGCTCCA
GGCAAGAATC
CTGGCTGTGG
AAAGATACCT
AAAGGATCAA
CAGCTCCTGG
GGATTTGGGG
TTGCTCTGGA
AAACTCATTT
GCACCACTGC
TGTGCCTTGG
AATGCTAGTT
GGAGTAATAA
ATCTCTGGAA
CAGATTTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGCCT
TTAAAAGAAA
AGGGGGGATT
GGGGGGTACA
GTGCAGGGGA
AAGAATAGTA
GACATAATAG
CAACAGACAT
ACAAACTAAA
GAATTACAAA
AACAAATTAC
AAAAATTCAA
AATTTTCGGG
TTTATTACAG
GGACAGCAGA
GATCCAGTTT
ATCGATAAGC
TTGGGAGTTC
CG