名称: | pHT304-plcA(Bacillus) |
---|---|
别称: | - |
基因长: | 180bp |
质粒宿主: | 枯草杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 芽孢杆菌 |
原核抗性: | Amp |
筛选标记: | Ery |
荧光标记: | |
启动子: | plcA |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pHT304 |
正向引物: | Forward(left ITR) |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCATCCTCCA
AAGTTGGAGA
GTGAGTTTTA
TGTCGCAAAT
ATTAATGTTT
CTGGTGAACC
TTATCAAATT
TTCGTTGATT
TAATAGAAAC
ATAGCGGTAA
AATTAGCAGT
AACTTAATAG
AACGGAAATG
AAAAAAGCCA
CTCTCATATG
CTATTGGCTA
CCAACCTTTA
GCGAGAATGA
CTTAATCCTG
TACAGCCATA
CAGGACTTCG
ACTTATAAGA
GGCGCCAACC
TCAAATAAGT
TATTTGCCTT
GTTTTCGCGA
ACAAGGCTTA
TTAGATACAC
CTATTGTACC
GTTACTCTAC
GAATATTTCA
ACTAGTAATT
ACTAGCATTG
TCATATACAT
AATAAAACGG
ATATAAAAGG
GCGTTTTCTA
TACCTAGAAG
TCTTGTAAAT
GTACAGGGCG
TTTAGATATA
GAGAACGCCC
TTTTTGTGTT
CCGTTCCAGT
GGAGGCTACC
ACTTTAAAAA
GATGGTCTAG
TGTAGCCAAT
GCAGGAGAGT
ACACTCGGAT
ATCAGTTGTC
GTTGCATTCA
ACTGTCTGAC
GTAAGCGAGG
TAAAGGACAC
AAGCCTTGCA
TAAAACAAGC
CTACGGGATG
TAAATCCTAA
TAATGATGAT
AACCAAGACG
TTAGCGGCAA
AAAGTGTTGG
GGGTTCAAAA
TAAGACATGA
TTGTGCGACT
GGAGTTAAAC
AGTTACTCGT
AAGCGGCGAT
CATGACACTG
ATTCACGGCT
ATTCTTGTAC
AAACTTTATT
ACAAGGATAT
GCGGGTTATA
TAGCGAATCA
CCCGAAAGGG
AACGGTGTTG
GGCGTGAGAA
ACGCACCGTA
CGGCGCAATA
CAATGCCAAT
AAGCTATATA
CGGACGGTAT
AGTAGTTTTG
TAAGCTATAA
CCGTTTGTCG
TCAATGCAAC
CAATCTCAAT
TCGAGACCTC
GGCATCTAAG
CCAGTACGAA
TGAGTGGGCG
TTTTAACCTC
GTAAATTTTC
AACAGGGGTT
ACTATGCCCA
AAACTACATT
CAGATTTCCT
AACAAACTCG
CCAGTATGAA
AACCTTAAGA
CCTTAAAGTC
AAGGGATTTG
AAGGATTTTA
ACCTCGATTA
GCAAAAAATG
TAGAGTACTG
AAGCAACTAC
CATTAACTAA
GATAGTGGGG
GATTGAGGAA
GAATCCAGAG
CTGTTTAAAT
CAAGTGAAAG
ACAAGATGAA
ATTAAAAGAA
TAGTGAAAGA
TAGGGGAGTG
GTTCTCTATG
AGAAAGGAAA
TGGCTAGAGA
ACAAAGGCAG
CGGTTTATTG
ATCTATTGTT
AGACTTTATG
GTAAAGAATC
CTCATTTATT
TGTTAATGGT
ACAGAGGATG
AAAGTAATAA
TGTTGTTACA
AAATGTAATA
GTGATATTAA
AGAGGTTGCG
GAGTCATATT
TAACTCTTTT
ATAGTGAGAG
GGTTAAAACT
AATTAATATG
TATTAAGGCC
CAATGTTGGA
ATTATTGTAT
TTCACTAGGC
AACCTACTTA
CTAAAAGTAA
GATTATCCAT
TAGTGGATGT
TATAATATTG
GGTTTTTTAA
CACAATAATC
ATCGCCTTTC
GGTGTCGTTT
GATAGAAAAG
TAACCATTAG
CGATGAAAAA
GTCAATATAA
AAAGCCATCC
GTAAAAAACG
GATGGCTTAC
CGTACATAGG
ATCGTTGGTA
GGGCGGCGTA
TCCTACATCT
CTGGTAACTG
ACCTAGCCAA
TCAAATGCTT
GAGAACGGCG
GTTAGATAAG
CGCGTGGGGA
ACCTTTCCCA
CCTCAAAGAT
CCTATATCAT
TATTATGTTA
CTTTCTACAG
GTAGTATACC
ATGTTCTTAT
ATTTTAGTAA
ACTCCCCGTT
AGCTTAACAG
GTCTTTGTAA
GCAATTAAAC
GTCCACTATT
CAATCGTCTT
TGGATTTTCG
CAGGACCGTT
TTTTAGATCG
AACATAGTTG
ATAAGAACAA
ATAACCGCTT
GGGTCCAACT
TTATAGCAAT
TAGTATATGG
TCATTTAAAA
TCTTTACCAA
TTCAACGCTA
TTAGGTTCTT
TAGGATTTTG
CCCGACATAG
TCGGGGTGTT
CAACGATATC
TTTTATGTGC
GATGAATATT
TTTCATAAAT
ACCAGGATGT
TGTTTCTTTA
CGTGCTTTAT
AAATCCGGGA
AACATTTTTA
CATCGTTAGA
AGTGCAAGTC
AAGTTATATG
TATCTATAAT
GATTTGTGGA
AGTTTTGCCA
CAACAGTTGG
TTTATTTACA
ATCTTTTTTT
TATTAGCCGT
CAAATTTCTC
CCTCATCTCG
TCTCTTTATA
TCTTTATTTT
ATCATAAAGG
AGTATTTGAA
CCGTCGCGCG
GGACAGGTTT
ATGATAGGGA
TATTTTATTG
AATAATTGAT
GGTATAAGGG
ACTTTCATGC
TTGGAAAGTG
GGGATTATGA
ATTAGATGCT
TGTCCACAAT
ATGTTCCAAT
GTAATTAAAA
TTTATGTTCC
CACCTTGACC
AAACATCACG
TCCATACTTA
AATCGTCCCT
CCTTTAATAG
GTAAAATATT
AATTTACCTT
AATAAAAAAA
TAATGGATAA
TAGTATTCGT
CTGAATTTAT
ATAATCAGGG
GGAACTATTG
ATGCTGGGGA
TACTATTTAC
AGCGGCGCCA
TCTACTGATG
TCGTAAAGGA
TTTGCAAGAT
AAAGTTATAT
CATTGCAGGA
TCATGAGGTA
GCGTTTTTGA
ACACCACGAT
ATCTAATATG
TTGATCCCCT
TAGAAGCAAA
CTTAAGAGTG
TGTTGATAGT
GCAGTATCTT
AAAATTTTGT
GTATAATAGG
AATTGAAGTT
AAATTAGATG
CTAAAAATTT
GTAATTAAGA
AGGAGGGATT
CGTCATGTTG
GTATTCCAAA
TGCGTAATGT
AGATAAAACA
TCTACTGTTT
TGAAACAGAC
TAAAAACAGT
GATTACGCAG
ATAAATAAAT
ACGTTAGATT
AATTCCTACC
AGTGACTAAT
CTTATGACTT
TTTAAACAGA
TAACTAAAAT
TACAAACAAA
TCGTTTAACT
TCTGTATTTA
TTTACAGATG
TAATCACTTC
AGGAGTAATT
ACATGAACAA
AAATATAAAA
TATTCTCAAA
ACTTTTTAAC
GAGTGAAAAA
GTACTCAACC
AAATAATAAA
ACAATTGAAT
TTAAAAGAAA
CCGATACCGT
TTACGAAATT
GGAACAGGTA
AAGGGCATTT
AACGACGAAA
CTGGCTAAAA
TAAGTAAACA
GGTAACGTCT
ATTGAATTAG
ACAGTCATCT
ATTCAACTTA
TCGACAGAAA
AATTAAAACT
GAACATTCGT
GTCACTTTAA
TTCACCAAGA
TATTCTACAG
TTTCAATTCC
CTAACAAACA
GAGGTATAAA
ATTGTTGGGA
GTATTCCTTA
CCATTTAAGC
ACACAAATTA
TTAAAAAAGT
GGTTTTTGAA
AGCCATGCGT
CTGACATCTA
TCTGATTGTT
GAAGAAGGAT
TCTACAAGCG
TACCTTGGAT
ATTCACCGAA
CACTAGGGTT
GCTCTTGCAC
ACTCAAGTCT
CGATTCAGCA
ATTGCTTAAG
CTGCCAGCGG
AATGCTTTCA
TCCTAAACCA
AAAGTAAACA
GTGTCTTAAT
AAAACTTACC
CGCCATACCA
CAGATGTTCC
AGATAAATAT
TGGAAGCTAT
ATACGTACTT
TGTTTCAAAA
TGGGTCAATC
GAGAATATCG
TCAACTGTTT
ACTAAAAATC
AGTTTCATCA
AGCAATGAAA
CACGCCAAAG
TAAACAATTT
AAGTACCATT
ACTTATGAGC
AAGTATTGTC
TATTTTTAAT
AGTTATCTAT
TATTTAACGG
GAGGAAATAA
TTCTATGAGT
CGCTTTTTTA
AATTTGGAAA
GTTACACGTT
ACTAAAGGGA
ATGGAGATAA
ATTATTAGAT
ATACTACTGA
CAGCTTCCAA
GAAGGTAAAG
AGGTCCCTAG
CGCCTACGGG
GAATTTGTAT
CGGGATTGAA
AAAGGAAGAG
TATGAGTATT
CAACATTTCC
GTGTCGCCCT
TATTCCCTTT
TTTGCGGCAT
TTTGCCTTCC
TGTTTTTGCT
CACCCAGAAA
CGCTGGTGAA
AGTAAAAGAT
GCTGAAGATC
AGTTGGGTGC
ACGAGTGGGT
TACATCGAAC
TGGATCTCAA
CAGCGGTAAG
ATCCTTGAGA
GTTTTCGCCC
CGAAGAACGT
TTTCCAATGA
TGAGCACTTT
TAAAGTTCTG
CTATGTGGCG
CGGTATTATC
CCGTATTGAC
GCCGGGCAAG
AGCAACTCGG
TCGCCGCATA
CACTATTCTC
AGAATGACTT
GGTTGAGTAC
TCACCAGTCA
CAGAAAAGCA
TCTTACGGAT
GGCATGACAG
TAAGAGAATT
ATGCAGTGCT
GCCATAACCA
TGAGTGATAA
CACTGCGGCC
AACTTACTTC
TGACAACGAT
CGGAGGACCG
AAGGAGCTAA
CCGCTTTTTT
GCACAACATG
GGGGATCATG
TAACTCGCCT
TGATCGTTGG
GAACCGGAGC
TGAATGAAGC
CATACCAAAC
GACGAGCGTG
ACACCACGAT
GCCTGTAGCA
ATGGCAACAA
CGTTGCGCAA
ACTATTAACT
GGCGAACTAC
TTACTCTAGC
TTCCCGGCAA
CAATTAATAG
ACTGGATGGA
GGCGGATAAA
GTTGCAGGAC
CACTTCTGCG
CTCGGCCCTT
CCGGCTGGCT
GGTTTATTGC
TGATAAATCT
GGAGCCGGTG
AGCGTGGGTC
TCGCGGTATC
ATTGCAGCAC
TGGGGCCAGA
TGGTAAGCCC
TCCCGTATCG
TAGTTATCTA
CACGACGGGG
AGTCAGGCAA
CTATGGATGA
ACGAAATAGA
CAGATCGCTG
AGATAGGTGC
CTCACTGATT
AAGCATTGGT
AACTGTCAGA
CCAAGTTTAC
TCATATATAC
TTTAGATTGA
TTTAAAACTT
CATTTTTAAT
TTAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TCTTGAGATC
CTTTTTTTCT
GCGCGTAATC
TGCTGCTTGC
AAACAAAAAA
ACCACCGCTA
CCAGCGGTGG
TTTGTTTGCC
GGATCAAGAG
CTACCAACTC
TTTTTCCGAA
GGTAACTGGC
TTCAGCAGAG
CGCAGATACC
AAATACTGTC
CTTCTAGTGT
AGCCGTAGTT
AGGCCACCAC
TTCAAGAACT
CTGTAGCACC
GCCTACATAC
CTCGCTCTGC
TAATCCTGTT
ACCAGTGGCT
GCTGCCAGTG
GCGATAAGTC
GTGTCTTACC
GGGTTGGACT
CAAGACGATA
GTTACCGGAT
AAGGCGCAGC
GGTCGGGCTG
AACGGGGGGT
TCGTGCACAC
AGCCCAGCTT
GGAGCGAACG
ACCTACACCG
AACTGAGATA
CCTACAGCGT
GAGCTATGAG
AAAGCGCCAC
GCTTCCCGAA
GGGAGAAAGG
CGGACAGGTA
TCCGGTAAGC
GGCAGGGTCG
GAACAGGAGA
GCGCACGAGG
GAGCTTCCAG
GGGGAAACGC
CTGGTATCTT
TATAGTCCTG
TCGGGTTTCG
CCACCTCTGA
CTTGAGCGTC
GATTTTTGTG
ATGCTCGTCA
GGGGGGCGGA
GCCTATGGAA
AAACGCCAGC
AACGCGGCCT
TTTTACGGTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTTCTTTCCT
GCGTTATCCC
CTGATTCTGT
GGATAACCGT
ATTACCGCCT
TTGAGTGAGC
TGATACCGCT
CGCCGCAGCC
GAACGACCGA
GCGCAGCGAG
TCAGTGAGCG
AGGAAGCGGA
AGAGCGCCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCTG
GCACGACAGG
TTTCCCGACT
GGAAAGCGGG
CAGTGAGCGC
AACGCAATAG
CAAAAGGAAC
TTCAGATGAA
GTTCCTTTAA
ATTTTTCTAA
TTTAGTATAT
GTGAATGATT
TTTTAACTTT
ACTTCTATGC
AATATTTCAT
ATTGATGAAA
TGTTATTTAT
TTTTTATGAA
CTAGCTTATA
TTATATTCAA
TAAGTTTGAT
GTAAAAACAG
AAAAAGTGAG
GGATAATATT
TCACACAGGA
AACAGCTATG
ACCATGATTA
CGCCAAGCTT
ATGGTCGTTT
TACAACGTCG
TGACTGGGAA
AACCCTGGCG
TTACCCAACT
TAATCGCCTT
GCAGCACATC
CCCCTTTCGC
CAGCTGGCGT
AATAGCGAAG
AGGCCCGCAC
CGATCGCCCT
TCCCAACAGT
TGCGCAGCCT
GAATGGCGAA
TGGCGCTTTG
CCTGGTTTCC
GGCACCAGAA
GCGGTGCCGG
AAAGCTGGCT
GGAGTGCGAT
CTTCCTGAGG
CCGATACTGT
CGTCGTCCCC
TCAAACTGGC
AGATGCACGG
TTACGATGCG
CCCATCTACA
CCAACGTGAC
CTATCCCATT
ACGGTCAATC
CGCCGTTTGT
TCCCACGGAG
AATCCGACGG
GTTGTTACTC
GCTCACATTT
AATGTTGATG
AAAGCTGGCT
ACAGGAAGGC
CAGACGCGAA
TTATTTTTGA
TGGCGTTAAC
TCGGCGTTTC
ATCTGTGGTG
CAACGGGCGC
TGGGTCGGTT
ACGGCCAGGA
CAGTCGTTTG
CCGTCTGAAT
TTGACCTGAG
CGCATTTTTA
CGCGCCGGAG
AAAACCGCCT
CGCGGTGATG
GTGCTGCGCT
GGAGTGACGG
CAGTTATCTG
GAAGATCAGG
ATATGTGGCG
GATGAGCGGC
ATTTTCCGTG
ACGTCTCGTT
GCTGCATAAA
CCGACTACAC
AAATCAGCGA
TTTCCATGTT
GCCACTCGCT
TTAATGATGA
TTTCAGCCGC
GCTGTACTGG
AGGCTGAAGT
TCAGATGTGC
GGCGAGTTGC
GTGACTACCT
ACGGGTAACA
GTTTCTTTAT
GGCAGGGTGA
AACGCAGGTC
GCCAGCGGCA
CCGCGCCTTT
CGGCGGTGAA
ATTATCGATG
AGCGTGGTGG
TTATGCCGAT
CGCGTCACAC
TACGTCTGAA
CGTCGAAAAC
CCGAAACTGT
GGAGCGCCGA
AATCCCGAAT
CTCTATCGTG
CGGTGGTTGA
ACTGCACACC
GCCGACGGCA
CGCTGATTGA
AGCAGAAGCC
TGCGATGTCG
GTTTCCGCGA
GGTGCGGATT
GAAAATGGTC
TGCTGCTGCT
GAACGGCAAG
CCGTTGCTGA
TTCGAGGCGT
TAACCGTCAC
GAGCATCATC
CTCTGCATGG
TCAGGTCATG
GATGAGCAGA
CGATGGTGCA
GGATATCCTG
CTGATGAAGC
AGAACAACTT
TAACGCCGTG
CGCTGTTCGC
ATTATCCGAA
CCATCCGCTG
TGGTACACGC
TGTGCGACCG
CTACGGCCTG
TATGTGGTGG
ATGAAGCCAA
TATTGAAACC
CACGGCATGG
TGCCAATGAA
TCGTCTGACC
GATGATCCGC
GCTGGCTACC
GGCGATGAGC
GAACGCGTAA
CGCGAATGGT
GCAGCGCGAT
CGTAATCACC
CGAGTGTGAT
CATCTGGTCG
CTGGGGAATG
AATCAGGCCA
CGGCGCTAAT
CACGACGCGC
TGTATCGCTG
GATCAAATCT
GTCGATCCTT
CCCGCCCGGT
GCAGTATGAA
GGCGGCGGAG
CCGACACCAC
GGCCACCGAT
ATTATTTGCC
CGATGTACGC
GCGCGTGGAT
GAAGACCAGC
CCTTCCCGGC
TGTGCCGAAA
TGGTCCATCA
AAAAATGGCT
TTCGCTACCT
GGAGAGACGC
GCCCGCTGAT
CCTTTGCGAA
TACGCCCACG
CGATGGGTAA
CAGTCTTGGC
GGTTTCGCTA
AATACTGGCA
GGCGTTTCGT
CAGTATCCCC
GTTTACAGGG
CGGCTTCGTC
TGGGACTGGG
TGGATCAGTC
GCTGATTAAA
TATGATGAAA
ACGGCAACCC
GTGGTCGGCT
TACGGCGGTG
ATTTTGGCGA
TACGCCGAAC
GATCGCCAGT
TCTGTATGAA
CGGTCTGGTC
TTTGCCGACC
GCACGCCGCA
TCCAGCGCTG
ACGGAAGCAA
AACACCAGCA
GCAGTTTTTC
CAGTTCCGTT
TATCCGGGCA
AACCATCGAA
GTGACCAGCG
AATACCTGTT
CCGTCATAGC
GATAACGAGC
TCCTGCACTG
GATGGTGGCG
CTGGATGGTA
AGCCGCTGGC
AAGCGGTGAA
GTGCCTCTGG
ATGTCGCTCC
ACAAGGTAAA
CAGTTGATTG
AACTGCCTGA
ACTACCGCAG
CCGGAGAGCG
CCGGGCAACT
CTGGCTCACA
GTACGCGTAG
TGCAACCGAA
CGCGACCGCA
TGGTCAGAAG
CCGGGCACAT
CAGCGCCTGG
CAGCAGTGGC
GTCTGGCGGA
AAACCTCAGT
GTGACGCTCC
CCGCCGCGTC
CCACGCCATC
CCGCATCTGA
CCACCAGCGA
AATGGATTTT
TGCATCGAGC
TGGGTAATAA
GCGTTGGCAA
TTTAACCGCC
AGTCAGGCTT
TCTTTCACAG
ATGTGGATTG
GCGATAAAAA
CCAACTGCTG
ACGCCGCTGC
GCGATCAGTT
CACCCGTGCA
CCGCTGGATA
ACGACATTGG
CGTAAGTGAA
GCGACCCGCA
TTGACCCTAA
CGCCTGGGTC
GAACGCTGGA
AGGCGGCGGG
CCATTACCAG
GCCGAAGCAG
CGTTGTTGCA
GTGCACGGCA
GATACACTTG
CTGATGCGGT
GCTGATTACG
ACCGCTCACG
CGTGGCAGCA
TCAGGGGAAA
ACCTTATTTA
TCAGCCGGAA
AACCTACCGG
ATTGATGGTA
GTGGTCAAAT
GGCGATTACC
GTTGATGTTG
AAGTGGCGAG
CGATACACCG
CATCCGGCGC
GGATTGGCCT
GAACTGCCAG
CTGGCGCAGG
TAGCAGAGCG
GGTAAACTGG
CTCGGATTAG
GGCCGCAAGA
AAACTATCCC
GACCGCCTTA
CTGCCGCCTG
TTTTGACCGC
TGGGATCTGC
CATTGTCAGA
CATGTATACC
CCGTACGTCT
TCCCGAGCGA
AAACGGTCTG
CGCTGCGGGA
CGCGCGAATT
GAATTATGGC
CCACACCAGT
GGCGCGGCGA
CTTCCAGTTC
AACATCAGCC
GCTACAGTCA
ACAGCAACTG
ATGGAAACCA
GCCATCGCCA
TCTGCTGCAC
GCGGAAGAAG
GCACATGGCT
GAATATCGAC
GGTTTCCATA
TGGGGATTGG
TGGCGACGAC
TCCTGGAGCC
CGTCAGTATC
GGCGGAATTC
CAGCTGAGCG
CCGGTCGCTA
CCATTACCAG
TTGGTCTGGT
GTCAAAAATA
AGAATTCACT
GGCCGTCGTT
TTACAACGTC
GTGACTGGGA
AAACCCTGGC
GTTACCCAAC
TTAATCGCCT
TGCAGCACAT
CCCCCTTTCG
CCAGCTGGCG
TAATAGCGAA
GAGGCCCGCA
CCGATCGCCC
TTCCCAACAG
TTGCGCAGCC
TGAATGGCGA
ATGGCGCCTG
ATGCGGTATT
TTCTCCTTAC
GCATCTGTGC
GGTATTTCAC
ACCGCATATG
GTGCACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGCCCC
GACACCCGCC
AACACCCGCT
GACGCGCCCT
GACGGGCTTG
TCTGCTCCCG
GCATCCGCTT
ACAGACAAGC
TGTGACCGTC
TCCGGGAGCT
GCATGTGTCA
GAGGTTTTCA
CCGTCATCAC
CGAAACGCGC
GAGACGAAAG
GGCCTCGTGA
TACGCCTATT
TTTATAGGTT
AATGTCATGA
TAATAATGGT
TTCTTAGACG
TCAGGTGGCA
CTTTTCGGGG
AAATGTGCGC
GGAACCCCTA
TTTGTTTATT
TTTCTAAATA
CATTCAAATA
TGTATCCGCT
CATGAGACAA
TAACCCTGAT
AAATGCTTCA
ATAAT