名称: | pCMV-HA-Cep164(mouse)-mCherry-Neo |
---|---|
别称: | NM_001081373.3;D030051D21 |
基因长: | 3999bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 红色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pmCherry-N1 |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTGG
TTTAGTGAAC
CGTCAGATCC
GCTAGCGCTA
CCGGACTCAG
ATCTCGAGCT
CAAGCTTCGA
ATTCGCCACC
ATGTACCCAT
ACGACGTCCC
AGACTACGCT
ATGGCTAGAC
GGCCCATCCT
TTTAGGTGAC
CAGCTGGTCC
TGGAGGAAGA
TTCTGATGAG
ACCTACGTTC
CCAGTGAGCA
AGAAATCCTT
GACTTTGCCA
GAGTGATTGG
TATTGACCCT
ATCAAGGAAC
CAGAACTGAT
GTGGCTGGCG
AGAGAGGGTA
TTGAGGCTCC
ATTGCCCAAG
GGCTGGAAAC
CATGCCAGAA
CATCACAGGT
GATCTTTACT
ATTTCAACTT
TGACACGGGG
CAGTCCATCT
GGGACCATCC
ATGTGACGAG
CACTATCGAA
AGTTGGTCAT
CCAGGAGAGG
GAGAGGTGGT
CAGCTCCCGG
GGCCATTAAG
AAGAAAGACA
AGAAAAAGAA
AAAGGAAAAG
AAAAACAAGA
AGGACAAAGA
GACCTCCAAA
AGTCCTCTGG
TCCTGGGTTC
CCCCTTAGCT
CTAGTCCAGG
CCCCTCTTTG
GGGCCTGGCT
CCCTTACGAG
GCCTTGGGGA
TGCCCCACCC
TCTGCTCTTC
GTGGGTCGCA
GAGCGTGAGC
TTGGGCAGCT
CAGCGGATTC
TGGGCATCTT
GGGGAGCCCA
CGCTGCCTCC
ACAGGGTCTC
AAGGCTGCTG
CTTGTGCAAA
GGGTCTCTTG
GCTTCTGTCC
ATGAGGGCAA
GAATGCTCTC
AGCCTCTTGA
CTTTAGGGGA
AGAGACCAAC
GAGGAGGATG
AAGAGGAGAG
TGACAACCAG
AGTGTCCGGA
GCTCCAGTGA
ACTTCTCAAG
AACCTACATC
TGGACCTTGG
TGCCCTGGGA
GGTAACTTTG
AGTACGAGGA
GTCTCCAAGA
ACCAGCCAGC
CAGACAAGAA
AGATGTTTCT
CTGGACTCAG
ATGCCGATCG
CCCCCCAACT
CCCGGCAAGC
TGTTCAGCCA
GGGTGCAGAC
AGTAGTGTGG
CCAGTGCCAA
TGGCAGCAAG
TCCCAAGGAA
GAGGGGCAAG
TCCCTGGAAC
CCACAGAAAG
AAAACGAGAA
TAGTGATCCC
AAGGCTTCTT
CAAGTCAGAT
GGCTCCCGAG
TTGGACCCCG
GGGGTGATCA
GCCTTCCAGG
GCCTCTAAAA
AGCAGCAGGC
AGAGGACCCA
GTGCAGGCAG
GAAAGGAGGG
AGAGTGCAGG
AGGGAGAGTG
CAGCCAAGGA
GCCAAAGGAG
GCTTCTGCCT
TGGAGAACAC
CTCAGATGTC
AGTGAGGAGT
CAGAGATCCA
CGGACACTTG
AAGGACGCCC
GGCACTCAGG
GTCCGAAGCT
TCTGGCCCTA
AGTCTTTCCT
GGGCCTGGAC
TTGGGCTTTC
GCAGCCGCAT
CTCGGAGCAT
CTGCTGGATG
GTGACACACT
TTCTCCAGTC
TTGGGTGGAG
GCCACTGGGA
GGCCCAGGGG
TTAGACCAAG
AAGAACAAGA
TGACAGCAAA
TCCAGCATCG
CTGAGCCACA
GAGCAAGCAT
ACCCAAGGCT
CCGAGAGGGA
ACATCTCCAG
AGTAGCCTTC
ACAGCCAGGC
TACTGAGGAA
GGGCCTTTGC
AGACCCTGGA
GGGGCAGCCT
GAGTGGAAGG
AAGCAGAGGG
ACCTGGGAAG
GACTCTGTAG
CCAGCCCCGC
CCCACTGTCC
CTCCTCCAGA
GCCTCCTGAA
AGCACAGCTA
CAGAAAGCCA
CCGCTGAGGA
GAAGGAGAAG
GAAGAGGAGA
CTAAGATAAG
AGAGGAGGAA
AGCCGGAGGC
TGGTCTGCCT
CCGGGCCCAG
GTGCAGTCCA
GGACTGAGGC
TTTTGAGAAC
CAAATTAGAA
CCGAGCAACA
GGCTGCCCTA
CAGAGGCTTC
GAGAAGAGGC
CGAGACTCTC
CAGAAAGCTG
AAAGAGCCAG
CTTGGAACAG
AAAAGCAGGA
GGGCACTGGA
GCAGCTGAGG
GAGCAGCTGG
AGGCCGAGGA
GAGGAGTGCC
CAGGCTGCCT
TGAGGGCAGA
GAAGGAGGCG
GAGAAGGAGG
CCGCTCTGCT
GCAGCTGAGG
GAGCAGCTGG
AAGGGGAGAG
GAAGGAAGCA
GTGGCAGGCC
TAGAGAAGAA
GCACAGCGCT
GAGCTGGAAC
AGCTCTGTTC
CTCACTGGAA
GCCAAACACC
AGGAGGTGAT
CTCCAGCCTC
CAGAAGAAGA
TAGAGGGAGC
TCAGCAGAAA
GAGGAGGCCC
AATTACAGGA
GAGCCTTGGG
TGGGCCGAGC
AGAGAGCTCA
CCAGAAGGTT
CACCAGGTGA
CCGAGTATGA
ACAGGAGCTC
AGCAGCCTCC
TTCGAGACAA
GCGCCAGGAG
GTAGAGAGGG
AACACGAGCG
GAAGATGGAC
AAGATGAAGG
AGGAGCACTG
GCAAGAGATG
GCTGACGCCA
GAGAGCGATA
CGAAGCCGAG
GAGAGAAAGC
AGCGGGCTGA
CCTCCTGGGG
CACCTGACTG
GAGAGCTGGA
GCGCCTGCGA
AGGGCCCACG
AGCGGGAGTT
AGAGAGTATG
AGGCAGGAGC
AGGACCAGCA
GCTTGAAGAC
CTGAGGCGTC
GACACCGCGA
CCATGAAAGG
AAATTACAAG
ACTTAGAGGT
GGAACTCTCG
TCCAGAACTA
AAGATGTCAA
GGCCAGACTA
GCTCAGCTGA
ATGTTCAGGA
GGAGAACATA
CGGAAAGAGA
AGCAGCTGCT
CCTGGATGCG
CAGAGGCAGG
CTGCTCTGGA
GAGGGAGGAA
GCCACAGCCA
CCCACCAGCA
CCTGGAAGAA
GCCAAGAAAG
AGCACACACA
CCTGCTGGAG
ACGAAGCAGC
AGCTGCGAAG
GACCATCGAT
GACCTGAGGG
TCCGGCGGGT
GGAGCTGGAG
TCCCAAGTGG
ACCTGTTGCA
GGCGCAGAGT
CAGAGATTGC
AGAAGCACCT
GAGCAGCCTA
GAGGCCGAAG
TACAAAGGAA
GCAGGATGTC
TTGAAAGAGA
TGGCAGCTGA
GATGAATGCT
TCCCCACACC
CGGAGCCAGG
GCTCCACATT
GAGGACCTGA
GGAAATCCCT
TGACACAAAT
AAGAACCAAG
AGGTGTCCTC
TTCTCTCTCC
CTGAGTAAGG
AGGAGATCGA
CCTGTCTATG
GAGAGTGTCC
GACAGTTCCT
CTCTGCTGAG
GGTGTAGCTG
TGCGCAACGC
TAAGGAGTTC
CTGGTGCGCC
AGACACGCTC
CATGCGGAGG
CGACAGACAG
CTCTGAAAGC
CGCCCAGCAG
CACTGGCGCC
ATGAGCTGGC
CAGTGCCCAG
GAGGTAGACG
AAGACCTGCC
AGGCACCGAG
GTCCTGGGAA
ACATGCGCAA
GAACTTGAAT
GAGGAGACCA
GGCACCTGGA
TGAGATGAAG
TCAGCCATGC
GCAAAGGCCA
TGACTTGTTG
AAGAAGAAAG
AGGAGAAGCT
GATCCAACTG
GAATCTTCTC
TTCAGGAGGA
GGTCTCTGAC
GAGGACACTC
TGAAAGGATC
CTCTATCAAG
AAGGTGACCT
TTGATCTCAG
TGACATGGAC
GATTTGAGCA
GTGAGAGTCT
TGAGTCCAGC
CCCGTGCTGC
ACATCACCCC
GACCCCGACC
TCTGCTGATC
CCAACAAGAT
CCATTACCTG
AGCAGCTCCC
TTCAGCGAAT
CAGCAGTGAG
CTAAACGGCG
TGCTGAATGT
GCTGGGAAGC
CTCAACTCCC
AGCCGCCGCC
CCAGGGCCTC
GGCAGCCAGC
CCCCGCCTCC
ACTCTTCACC
TCCTCACTTC
GGTCCTCCAA
GAATGTCCTT
GACCCTGCCT
ACTCCTCCCA
GGCCAAGCTC
TCCTCCTTAT
CTTCCATCAC
ACCCATGTCC
ACCCAGTGGG
CCTGGGACCC
AGGGCAGGGC
ACCAAGCTGA
CCTCTTCCTC
CTCCTCTCAA
ACTGTGGATG
ATTTCCTGCT
GGAGAAGTGG
CGCAAGTATT
TTCCCTCTGG
CATCCCGTTG
CTCAGTGGCT
CTCCTCCCCC
ACCGGAGAAC
AAGCTGGGTT
ATGTGTCTGT
AAGTGAGCAG
CTCCACTTCC
TGCAGCGCTC
CCACCCTCGA
GTCCCCAGGA
CGGATGGTGT
CAGCATTCAG
AGCTTGATCG
ATTCCAACCG
GAAGTGGCTG
GAACATTTCC
GGAACGACCC
CAAGGTACAG
CTTTTCTCCT
CGGCGCCCAA
AGCAACAACC
ACATCGAACT
TATCGAACCT
CCTGCAGCTG
GGCCTGGACG
AGAACAACAG
ACTGAATGTG
TTTCATTACG
GATCCGGCGG
CGGAGGGAGT
GGAGGAGGGG
GAAGCATGGT
GAGCAAGGGC
GAGGAGGATA
ACATGGCCAT
CATCAAGGAG
TTCATGCGCT
TCAAGGTGCA
CATGGAGGGC
TCCGTGAACG
GCCACGAGTT
CGAGATCGAG
GGCGAGGGCG
AGGGCCGCCC
CTACGAGGGC
ACCCAGACCG
CCAAGCTGAA
GGTGACCAAG
GGTGGCCCCC
TGCCCTTCGC
CTGGGACATC
CTGTCCCCTC
AGTTCATGTA
CGGCTCCAAG
GCCTACGTGA
AGCACCCCGC
CGACATCCCC
GACTACTTGA
AGCTGTCCTT
CCCCGAGGGC
TTCAAGTGGG
AGCGCGTGAT
GAACTTCGAG
GACGGCGGCG
TGGTGACCGT
GACCCAGGAC
TCCTCCCTGC
AGGACGGCGA
GTTCATCTAC
AAGGTGAAGC
TGCGCGGCAC
CAACTTCCCC
TCCGACGGCC
CCGTAATGCA
GAAGAAGACC
ATGGGCTGGG
AGGCCTCCTC
CGAGCGGATG
TACCCCGAGG
ACGGCGCCCT
GAAGGGCGAG
ATCAAGCAGA
GGCTGAAGCT
GAAGGACGGC
GGCCACTACG
ACGCTGAGGT
CAAGACCACC
TACAAGGCCA
AGAAGCCCGT
GCAGCTGCCC
GGCGCCTACA
ACGTCAACAT
CAAGTTGGAC
ATCACCTCCC
ACAACGAGGA
CTACACCATC
GTGGAACAGT
ACGAACGCGC
CGAGGGCCGC
CACTCCACCG
GCGGCATGGA
CGAGCTGTAC
AAGTAGCGGC
CGCGACTCTA
GATCATAATC
AGCCATACCA
CATTTGTAGA
GGTTTTACTT
GCTTTAAAAA
ACCTCCCACA
CCTCCCCCTG
AACCTGAAAC
ATAAAATGAA
TGCAATTGTT
GTTGTTAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTAAG
GCGTAAATTG
TAAGCGTTAA
TATTTTGTTA
AAATTCGCGT
TAAATTTTTG
TTAAATCAGC
TCATTTTTTA
ACCAATAGGC
CGAAATCGGC
AAAATCCCTT
ATAAATCAAA
AGAATAGACC
GAGATAGGGT
TGAGTGTTGT
TCCAGTTTGG
AACAAGAGTC
CACTATTAAA
GAACGTGGAC
TCCAACGTCA
AAGGGCGAAA
AACCGTCTAT
CAGGGCGATG
GCCCACTACG
TGAACCATCA
CCCTAATCAA
GTTTTTTGGG
GTCGAGGTGC
CGTAAAGCAC
TAAATCGGAA
CCCTAAAGGG
AGCCCCCGAT
TTAGAGCTTG
ACGGGGAAAG
CCGGCGAACG
TGGCGAGAAA
GGAAGGGAAG
AAAGCGAAAG
GAGCGGGCGC
TAGGGCGCTG
GCAAGTGTAG
CGGTCACGCT
GCGCGTAACC
ACCACACCCG
CCGCGCTTAA
TGCGCCGCTA
CAGGGCGCGT
CAGGTGGCAC
TTTTCGGGGA
AATGTGCGCG
GAACCCCTAT
TTGTTTATTT
TTCTAAATAC
ATTCAAATAT
GTATCCGCTC
ATGAGACAAT
AACCCTGATA
AATGCTTCAA
TAATATTGAA
AAAGGAAGAG
TCCTGAGGCG
GAAAGAACCA
GCTGTGGAAT
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCG
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGG
CTTTTGCAAA
GATCGATCAA
GAGACAGGAT
GAGGATCGTT
TCGCATGATT
GAACAAGATG
GATTGCACGC
AGGTTCTCCG
GCCGCTTGGG
TGGAGAGGCT
ATTCGGCTAT
GACTGGGCAC
AACAGACAAT
CGGCTGCTCT
GATGCCGCCG
TGTTCCGGCT
GTCAGCGCAG
GGGCGCCCGG
TTCTTTTTGT
CAAGACCGAC
CTGTCCGGTG
CCCTGAATGA
ACTGCAAGAC
GAGGCAGCGC
GGCTATCGTG
GCTGGCCACG
ACGGGCGTTC
CTTGCGCAGC
TGTGCTCGAC
GTTGTCACTG
AAGCGGGAAG
GGACTGGCTG
CTATTGGGCG
AAGTGCCGGG
GCAGGATCTC
CTGTCATCTC
ACCTTGCTCC
TGCCGAGAAA
GTATCCATCA
TGGCTGATGC
AATGCGGCGG
CTGCATACGC
TTGATCCGGC
TACCTGCCCA
TTCGACCACC
AAGCGAAACA
TCGCATCGAG
CGAGCACGTA
CTCGGATGGA
AGCCGGTCTT
GTCGATCAGG
ATGATCTGGA
CGAAGAGCAT
CAGGGGCTCG
CGCCAGCCGA
ACTGTTCGCC
AGGCTCAAGG
CGAGCATGCC
CGACGGCGAG
GATCTCGTCG
TGACCCATGG
CGATGCCTGC
TTGCCGAATA
TCATGGTGGA
AAATGGCCGC
TTTTCTGGAT
TCATCGACTG
TGGCCGGCTG
GGTGTGGCGG
ACCGCTATCA
GGACATAGCG
TTGGCTACCC
GTGATATTGC
TGAAGAGCTT
GGCGGCGAAT
GGGCTGACCG
CTTCCTCGTG
CTTTACGGTA
TCGCCGCTCC
CGATTCGCAG
CGCATCGCCT
TCTATCGCCT
TCTTGACGAG
TTCTTCTGAG
CGGGACTCTG
GGGTTCGAAA
TGACCGACCA
AGCGACGCCC
AACCTGCCAT
CACGAGATTT
CGATTCCACC
GCCGCCTTCT
ATGAAAGGTT
GGGCTTCGGA
ATCGTTTTCC
GGGACGCCGG
CTGGATGATC
CTCCAGCGCG
GGGATCTCAT
GCTGGAGTTC
TTCGCCCACC
CTAGGGGGAG
GCTAACTGAA
ACACGGAAGG
AGACAATACC
GGAAGGAACC
CGCGCTATGA
CGGCAATAAA
AAGACAGAAT
AAAACGCACG
GTGTTGGGTC
GTTTGTTCAT
AAACGCGGGG
TTCGGTCCCA
GGGCTGGCAC
TCTGTCGATA
CCCCACCGAG
ACCCCATTGG
GGCCAATACG
CCCGCGTTTC
TTCCTTTTCC
CCACCCCACC
CCCCAAGTTC
GGGTGAAGGC
CCAGGGCTCG
CAGCCAACGT
CGGGGCGGCA
GGCCCTGCCA
TAGCCTCAGG
TTACTCATAT
ATACTTTAGA
TTGATTTAAA
ACTTCATTTT
TAATTTAAAA
GGATCTAGGT
GAAGATCCTT
TTTGATAATC
TCATGACCAA
AATCCCTTAA
CGTGAGTTTT
CGTTCCACTG
AGCGTCAGAC
CCCGTAGAAA
AGATCAAAGG
ATCTTCTTGA
GATCCTTTTT
TTCTGCGCGT
AATCTGCTGC
TTGCAAACAA
AAAAACCACC
GCTACCAGCG
GTGGTTTGTT
TGCCGGATCA
AGAGCTACCA
ACTCTTTTTC
CGAAGGTAAC
TGGCTTCAGC
AGAGCGCAGA
TACCAAATAC
TGTCCTTCTA
GTGTAGCCGT
AGTTAGGCCA
CCACTTCAAG
AACTCTGTAG
CACCGCCTAC
ATACCTCGCT
CTGCTAATCC
TGTTACCAGT
GGCTGCTGCC
AGTGGCGATA
AGTCGTGTCT
TACCGGGTTG
GACTCAAGAC
GATAGTTACC
GGATAAGGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCTA
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCCTGGC
CTTTTGCTGG
CCTTTTGCTC
ACATGTTCTT
TCCTGCGTTA
TCCCCTGATT
CTGTGGATAA
CCGTATTACC
GCCATGCAT