产品货号：P69886
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pU6-USP10(human)-sgRNA5-Cas9-EGFP |
|---|---|
| 别称: | GACTCCTCGATCTTCAGTTG |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | |
| 荧光标记： | 绿色 |
| 启动子: | U6 |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pX458 |
| 正向引物: | U6-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTGGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
GACTCCTCGA
TCTTCAGTTG
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTTTTA
GCGCGTGCGC
CAATTCTGCA
GACAAATGGC
TCTAGAGGTA
CCCGTTACAT
AACTTACGGT
AAATGGCCCG
CCTGGCTGAC
CGCCCAACGA
CCCCCGCCCA
TTGACGTCAA
TAGTAACGCC
AATAGGGACT
TTCCATTGAC
GTCAATGGGT
GGAGTATTTA
CGGTAAACTG
CCCACTTGGC
AGTACATCAA
GTGTATCATA
TGCCAAGTAC
GCCCCCTATT
GACGTCAATG
ACGGTAAATG
GCCCGCCTGG
CATTGTGCCC
AGTACATGAC
CTTATGGGAC
TTTCCTACTT
GGCAGTACAT
CTACGTATTA
GTCATCGCTA
TTACCATGGT
CGAGGTGAGC
CCCACGTTCT
GCTTCACTCT
CCCCATCTCC
CCCCCCTCCC
CACCCCCAAT
TTTGTATTTA
TTTATTTTTT
AATTATTTTG
TGCAGCGATG
GGGGCGGGGG
GGGGGGGGGG
GCGCGCGCCG
GGGGGGGGGG
GGGGGGGGGG
GGGGGGGGGG
GGGGCGAGGC
GGAGAGGTGC
GGCGGCAGCC
AATCAGAGCG
GCGCGCTCCG
AAAGTTTCCT
TTTATGGCGA
GGCGGCGGCG
GCGGCGGCCC
TATAAAAAGC
GAAGCGCGCG
GCGGGCGGGA
GTCGCTGCGC
GCTGCCTTCG
CCCCGTGCCC
CGCTCCGCCG
CCGCCTCGCG
CCGCCCGCCC
CGGCTCTGAC
TGACCGCGTT
ACTCCCACAG
GTGAGCGGGC
GGGACGGCCC
TTCTCCTCCG
GGCTGTAATT
AGCTGAGCAA
GAGGTAAGGG
TTTAAGGGAT
GGTTGGTTGG
TGGGGTATTA
ATGTTTAATT
ACCTGGAGCA
CCTGCCTGAA
ATCACTTTTT
TTCAGGTTGG
ACCGGTGCCA
CCATGGACTA
TAAGGACCAC
GACGGAGACT
ACAAGGATCA
TGATATTGAT
TACAAAGACG
ATGACGATAA
GATGGCCCCA
AAGAAGAAGC
GGAAGGTCGG
TATCCACGGA
GTCCCAGCAG
CCGACAAGAA
GTACAGCATC
GGCCTGGACA
TCGGCACCAA
CTCTGTGGGC
TGGGCCGTGA
TCACCGACGA
GTACAAGGTG
CCCAGCAAGA
AATTCAAGGT
GCTGGGCAAC
ACCGACCGGC
ACAGCATCAA
GAAGAACCTG
ATCGGAGCCC
TGCTGTTCGA
CAGCGGCGAA
ACAGCCGAGG
CCACCCGGCT
GAAGAGAACC
GCCAGAAGAA
GATACACCAG
ACGGAAGAAC
CGGATCTGCT
ATCTGCAAGA
GATCTTCAGC
AACGAGATGG
CCAAGGTGGA
CGACAGCTTC
TTCCACAGAC
TGGAAGAGTC
CTTCCTGGTG
GAAGAGGATA
AGAAGCACGA
GCGGCACCCC
ATCTTCGGCA
ACATCGTGGA
CGAGGTGGCC
TACCACGAGA
AGTACCCCAC
CATCTACCAC
CTGAGAAAGA
AACTGGTGGA
CAGCACCGAC
AAGGCCGACC
TGCGGCTGAT
CTATCTGGCC
CTGGCCCACA
TGATCAAGTT
CCGGGGCCAC
TTCCTGATCG
AGGGCGACCT
GAACCCCGAC
AACAGCGACG
TGGACAAGCT
GTTCATCCAG
CTGGTGCAGA
CCTACAACCA
GCTGTTCGAG
GAAAACCCCA
TCAACGCCAG
CGGCGTGGAC
GCCAAGGCCA
TCCTGTCTGC
CAGACTGAGC
AAGAGCAGAC
GGCTGGAAAA
TCTGATCGCC
CAGCTGCCCG
GCGAGAAGAA
GAATGGCCTG
TTCGGAAACC
TGATTGCCCT
GAGCCTGGGC
CTGACCCCCA
ACTTCAAGAG
CAACTTCGAC
CTGGCCGAGG
ATGCCAAACT
GCAGCTGAGC
AAGGACACCT
ACGACGACGA
CCTGGACAAC
CTGCTGGCCC
AGATCGGCGA
CCAGTACGCC
GACCTGTTTC
TGGCCGCCAA
GAACCTGTCC
GACGCCATCC
TGCTGAGCGA
CATCCTGAGA
GTGAACACCG
AGATCACCAA
GGCCCCCCTG
AGCGCCTCTA
TGATCAAGAG
ATACGACGAG
CACCACCAGG
ACCTGACCCT
GCTGAAAGCT
CTCGTGCGGC
AGCAGCTGCC
TGAGAAGTAC
AAAGAGATTT
TCTTCGACCA
GAGCAAGAAC
GGCTACGCCG
GCTACATTGA
CGGCGGAGCC
AGCCAGGAAG
AGTTCTACAA
GTTCATCAAG
CCCATCCTGG
AAAAGATGGA
CGGCACCGAG
GAACTGCTCG
TGAAGCTGAA
CAGAGAGGAC
CTGCTGCGGA
AGCAGCGGAC
CTTCGACAAC
GGCAGCATCC
CCCACCAGAT
CCACCTGGGA
GAGCTGCACG
CCATTCTGCG
GCGGCAGGAA
GATTTTTACC
CATTCCTGAA
GGACAACCGG
GAAAAGATCG
AGAAGATCCT
GACCTTCCGC
ATCCCCTACT
ACGTGGGCCC
TCTGGCCAGG
GGAAACAGCA
GATTCGCCTG
GATGACCAGA
AAGAGCGAGG
AAACCATCAC
CCCCTGGAAC
TTCGAGGAAG
TGGTGGACAA
GGGCGCTTCC
GCCCAGAGCT
TCATCGAGCG
GATGACCAAC
TTCGATAAGA
ACCTGCCCAA
CGAGAAGGTG
CTGCCCAAGC
ACAGCCTGCT
GTACGAGTAC
TTCACCGTGT
ATAACGAGCT
GACCAAAGTG
AAATACGTGA
CCGAGGGAAT
GAGAAAGCCC
GCCTTCCTGA
GCGGCGAGCA
GAAAAAGGCC
ATCGTGGACC
TGCTGTTCAA
GACCAACCGG
AAAGTGACCG
TGAAGCAGCT
GAAAGAGGAC
TACTTCAAGA
AAATCGAGTG
CTTCGACTCC
GTGGAAATCT
CCGGCGTGGA
AGATCGGTTC
AACGCCTCCC
TGGGCACATA
CCACGATCTG
CTGAAAATTA
TCAAGGACAA
GGACTTCCTG
GACAATGAGG
AAAACGAGGA
CATTCTGGAA
GATATCGTGC
TGACCCTGAC
ACTGTTTGAG
GACAGAGAGA
TGATCGAGGA
ACGGCTGAAA
ACCTATGCCC
ACCTGTTCGA
CGACAAAGTG
ATGAAGCAGC
TGAAGCGGCG
GAGATACACC
GGCTGGGGCA
GGCTGAGCCG
GAAGCTGATC
AACGGCATCC
GGGACAAGCA
GTCCGGCAAG
ACAATCCTGG
ATTTCCTGAA
GTCCGACGGC
TTCGCCAACA
GAAACTTCAT
GCAGCTGATC
CACGACGACA
GCCTGACCTT
TAAAGAGGAC
ATCCAGAAAG
CCCAGGTGTC
CGGCCAGGGC
GATAGCCTGC
ACGAGCACAT
TGCCAATCTG
GCCGGCAGCC
CCGCCATTAA
GAAGGGCATC
CTGCAGACAG
TGAAGGTGGT
GGACGAGCTC
GTGAAAGTGA
TGGGCCGGCA
CAAGCCCGAG
AACATCGTGA
TCGAAATGGC
CAGAGAGAAC
CAGACCACCC
AGAAGGGACA
GAAGAACAGC
CGCGAGAGAA
TGAAGCGGAT
CGAAGAGGGC
ATCAAAGAGC
TGGGCAGCCA
GATCCTGAAA
GAACACCCCG
TGGAAAACAC
CCAGCTGCAG
AACGAGAAGC
TGTACCTGTA
CTACCTGCAG
AATGGGCGGG
ATATGTACGT
GGACCAGGAA
CTGGACATCA
ACCGGCTGTC
CGACTACGAT
GTGGACCATA
TCGTGCCTCA
GAGCTTTCTG
AAGGACGACT
CCATCGACAA
CAAGGTGCTG
ACCAGAAGCG
ACAAGAACCG
GGGCAAGAGC
GACAACGTGC
CCTCCGAAGA
GGTCGTGAAG
AAGATGAAGA
ACTACTGGCG
GCAGCTGCTG
AACGCCAAGC
TGATTACCCA
GAGAAAGTTC
GACAATCTGA
CCAAGGCCGA
GAGAGGCGGC
CTGAGCGAAC
TGGATAAGGC
CGGCTTCATC
AAGAGACAGC
TGGTGGAAAC
CCGGCAGATC
ACAAAGCACG
TGGCACAGAT
CCTGGACTCC
CGGATGAACA
CTAAGTACGA
CGAGAATGAC
AAGCTGATCC
GGGAAGTGAA
AGTGATCACC
CTGAAGTCCA
AGCTGGTGTC
CGATTTCCGG
AAGGATTTCC
AGTTTTACAA
AGTGCGCGAG
ATCAACAACT
ACCACCACGC
CCACGACGCC
TACCTGAACG
CCGTCGTGGG
AACCGCCCTG
ATCAAAAAGT
ACCCTAAGCT
GGAAAGCGAG
TTCGTGTACG
GCGACTACAA
GGTGTACGAC
GTGCGGAAGA
TGATCGCCAA
GAGCGAGCAG
GAAATCGGCA
AGGCTACCGC
CAAGTACTTC
TTCTACAGCA
ACATCATGAA
CTTTTTCAAG
ACCGAGATTA
CCCTGGCCAA
CGGCGAGATC
CGGAAGCGGC
CTCTGATCGA
GACAAACGGC
GAAACCGGGG
AGATCGTGTG
GGATAAGGGC
CGGGATTTTG
CCACCGTGCG
GAAAGTGCTG
AGCATGCCCC
AAGTGAATAT
CGTGAAAAAG
ACCGAGGTGC
AGACAGGCGG
CTTCAGCAAA
GAGTCTATCC
TGCCCAAGAG
GAACAGCGAT
AAGCTGATCG
CCAGAAAGAA
GGACTGGGAC
CCTAAGAAGT
ACGGCGGCTT
CGACAGCCCC
ACCGTGGCCT
ATTCTGTGCT
GGTGGTGGCC
AAAGTGGAAA
AGGGCAAGTC
CAAGAAACTG
AAGAGTGTGA
AAGAGCTGCT
GGGGATCACC
ATCATGGAAA
GAAGCAGCTT
CGAGAAGAAT
CCCATCGACT
TTCTGGAAGC
CAAGGGCTAC
AAAGAAGTGA
AAAAGGACCT
GATCATCAAG
CTGCCTAAGT
ACTCCCTGTT
CGAGCTGGAA
AACGGCCGGA
AGAGAATGCT
GGCCTCTGCC
GGCGAACTGC
AGAAGGGAAA
CGAACTGGCC
CTGCCCTCCA
AATATGTGAA
CTTCCTGTAC
CTGGCCAGCC
ACTATGAGAA
GCTGAAGGGC
TCCCCCGAGG
ATAATGAGCA
GAAACAGCTG
TTTGTGGAAC
AGCACAAGCA
CTACCTGGAC
GAGATCATCG
AGCAGATCAG
CGAGTTCTCC
AAGAGAGTGA
TCCTGGCCGA
CGCTAATCTG
GACAAAGTGC
TGTCCGCCTA
CAACAAGCAC
CGGGATAAGC
CCATCAGAGA
GCAGGCCGAG
AATATCATCC
ACCTGTTTAC
CCTGACCAAT
CTGGGAGCCC
CTGCCGCCTT
CAAGTACTTT
GACACCACCA
TCGACCGGAA
GAGGTACACC
AGCACCAAAG
AGGTGCTGGA
CGCCACCCTG
ATCCACCAGA
GCATCACCGG
CCTGTACGAG
ACACGGATCG
ACCTGTCTCA
GCTGGGAGGC
GACAAAAGGC
CGGCGGCCAC
GAAAAAGGCC
GGCCAGGCAA
AAAAGAAAAA
GGAATTCGGC
AGTGGAGAGG
GCAGAGGAAG
TCTGCTAACA
TGCGGTGACG
TCGAGGAGAA
TCCTGGCCCA
GTGAGCAAGG
GCGAGGAGCT
GTTCACCGGG
GTGGTGCCCA
TCCTGGTCGA
GCTGGACGGC
GACGTAAACG
GCCACAAGTT
CAGCGTGTCC
GGCGAGGGCG
AGGGCGATGC
CACCTACGGC
AAGCTGACCC
TGAAGTTCAT
CTGCACCACC
GGCAAGCTGC
CCGTGCCCTG
GCCCACCCTC
GTGACCACCC
TGACCTACGG
CGTGCAGTGC
TTCAGCCGCT
ACCCCGACCA
CATGAAGCAG
CACGACTTCT
TCAAGTCCGC
CATGCCCGAA
GGCTACGTCC
AGGAGCGCAC
CATCTTCTTC
AAGGACGACG
GCAACTACAA
GACCCGCGCC
GAGGTGAAGT
TCGAGGGCGA
CACCCTGGTG
AACCGCATCG
AGCTGAAGGG
CATCGACTTC
AAGGAGGACG
GCAACATCCT
GGGGCACAAG
CTGGAGTACA
ACTACAACAG
CCACAACGTC
TATATCATGG
CCGACAAGCA
GAAGAACGGC
ATCAAGGTGA
ACTTCAAGAT
CCGCCACAAC
ATCGAGGACG
GCAGCGTGCA
GCTCGCCGAC
CACTACCAGC
AGAACACCCC
CATCGGCGAC
GGCCCCGTGC
TGCTGCCCGA
CAACCACTAC
CTGAGCACCC
AGTCCGCCCT
GAGCAAAGAC
CCCAACGAGA
AGCGCGATCA
CATGGTCCTG
CTGGAGTTCG
TGACCGCCGC
CGGGATCACT
CTCGGCATGG
ACGAGCTGTA
CAAGGAATTC
TAACTAGAGC
TCGCTGATCA
GCCTCGACTG
TGCCTTCTAG
TTGCCAGCCA
TCTGTTGTTT
GCCCCTCCCC
CGTGCCTTCC
TTGACCCTGG
AAGGTGCCAC
TCCCACTGTC
CTTTCCTAAT
AAAATGAGGA
AATTGCATCG
CATTGTCTGA
GTAGGTGTCA
TTCTATTCTG
GGGGGTGGGG
TGGGGCAGGA
CAGCAAGGGG
GAGGATTGGG
AAGAGAATAG
CAGGCATGCT
GGGGAGCGGC
CGCAGGAACC
CCTAGTGATG
GAGTTGGCCA
CTCCCTCTCT
GCGCGCTCGC
TCGCTCACTG
AGGCCGGGCG
ACCAAAGGTC
GCCCGACGCC
CGGGCTTTGC
CCGGGCGGCC
TCAGTGAGCG
AGCGAGCGCG
CAGCTGCCTG
CAGGGGCGCC
TGATGCGGTA
TTTTCTCCTT
ACGCATCTGT
GCGGTATTTC
ACACCGCATA
CGTCAAAGCA
ACCATAGTAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCTTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTTGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
TCTATCTCGG
GCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGT
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTTTA
ACAAAATATT
AACGTTTACA
ATTTTATGGT
GCACTCTCAG
TACAATCTGC
TCTGATGCCG
CATAGTTAAG
CCAGCCCCGA
CACCCGCCAA
CACCCGCTGA
CGCGCCCTGA
CGGGCTTGTC
TGCTCCCGGC
ATCCGCTTAC
AGACAAGCTG
TGACCGTCTC
CGGGAGCTGC
ATGTGTCAGA
GGTTTTCACC
GTCATCACCG
AAACGCGCGA
GACGAAAGGG
CCTCGTGATA
CGCCTATTTT
TATAGGTTAA
TGTCATGATA
ATAATGGTTT
CTTAGACGTC
AGGTGGCACT
TTTCGGGGAA
ATGTGCGCGG
AACCCCTATT
TGTTTATTTT
TCTAAATACA
TTCAAATATG
TATCCGCTCA
TGAGACAATA
ACCCTGATAA
ATGCTTCAAT
AATATTGAAA
AAGGAAGAGT
ATGAGTATTC
AACATTTCCG
TGTCGCCCTT
ATTCCCTTTT
TTGCGGCATT
TTGCCTTCCT
GTTTTTGCTC
ACCCAGAAAC
GCTGGTGAAA
GTAAAAGATG
CTGAAGATCA
GTTGGGTGCA
CGAGTGGGTT
ACATCGAACT
GGATCTCAAC
AGCGGTAAGA
TCCTTGAGAG
TTTTCGCCCC
GAAGAACGTT
TTCCAATGAT
GAGCACTTTT
AAAGTTCTGC
TATGTGGCGC
GGTATTATCC
CGTATTGACG
CCGGGCAAGA
GCAACTCGGT
CGCCGCATAC
ACTATTCTCA
GAATGACTTG
GTTGAGTACT
CACCAGTCAC
AGAAAAGCAT
CTTACGGATG
GCATGACAGT
AAGAGAATTA
TGCAGTGCTG
CCATAACCAT
GAGTGATAAC
ACTGCGGCCA
ACTTACTTCT
GACAACGATC
GGAGGACCGA
AGGAGCTAAC
CGCTTTTTTG
CACAACATGG
GGGATCATGT
AACTCGCCTT
GATCGTTGGG
AACCGGAGCT
GAATGAAGCC
ATACCAAACG
ACGAGCGTGA
CACCACGATG
CCTGTAGCAA
TGGCAACAAC
GTTGCGCAAA
CTATTAACTG
GCGAACTACT
TACTCTAGCT
TCCCGGCAAC
AATTAATAGA
CTGGATGGAG
GCGGATAAAG
TTGCAGGACC
ACTTCTGCGC
TCGGCCCTTC
CGGCTGGCTG
GTTTATTGCT
GATAAATCTG
GAGCCGGTGA
GCGTGGAAGC
CGCGGTATCA
TTGCAGCACT
GGGGCCAGAT
GGTAAGCCCT
CCCGTATCGT
AGTTATCTAC
ACGACGGGGA
GTCAGGCAAC
TATGGATGAA
CGAAATAGAC
AGATCGCTGA
GATAGGTGCC
TCACTGATTA
AGCATTGGTA
ACTGTCAGAC
CAAGTTTACT
CATATATACT
TTAGATTGAT
TTAAAACTTC
ATTTTTAATT
TAAAAGGATC
TAGGTGAAGA
TCCTTTTTGA
TAATCTCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTTC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
T
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