名称: | pGADT7-TYMS(human) |
---|---|
别称: | NM_001071.4;DKCD; HST422; TMS; TS |
基因长: | 942bp |
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | LEU2 |
荧光标记: | |
启动子: | ADH1 |
复制子: | 2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pGADT7 |
正向引物: | T7 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGCATGCCTG
CAGGTCGAGA
TCCGGGATCG
AAGAAATGAT
GGTAAATGAA
ATAGGAAATC
AAGGAGCATG
AAGGCAAAAG
ACAAATATAA
GGGTCGAACG
AAAAATAAAG
TGAAAAGTGT
TGATATGATG
TATTTGGCTT
TGCGGCGCCG
AAAAAACGAG
TTTACGCAAT
TGCACAATCA
TGCTGACTCT
GTGGCGGACC
CGCGCTCTTG
CCGGCCCGGC
GATAACGCTG
GGCGTGAGGC
TGTGCCCGGC
GGAGTTTTTT
GCGCCTGCAT
TTTCCAAGGT
TTACCCTGCG
CTAAGGGGCG
AGATTGGAGA
AGCAATAAGA
ATGCCGGTTG
GGGTTGCGAT
GATGACGACC
ACGACAACTG
GTGTCATTAT
TTAAGTTGCC
GAAAGAACCT
GAGTGCATTT
GCAACATGAG
TATACTAGAA
GAATGAGCCA
AGACTTGCGA
GACGCGAGTT
TGCCGGTGGT
GCGAACAATA
GAGCGACCAT
GACCTTGAAG
GTGAGACGCG
CATAACCGCT
AGAGTACTTT
GAAGAGGAAA
CAGCAATAGG
GTTGCTACCA
GTATAAATAG
ACAGGTACAT
ACAACACTGG
AAATGGTTGT
CTGTTTGAGT
ACGCTTTCAA
TTCATTTGGG
TGTGCACTTT
ATTATGTTAC
AATATGGAAG
GGAACTTTAC
ACTTCTCCTA
TGCACATATA
TTAATTAAAG
TCCAATGCTA
GTAGAGAAGG
GGGGTAACAC
CCCTCCGCGC
TCTTTTCCGA
TTTTTTTCTA
AACCGTGGAA
TATTTCGGAT
ATCCTTTTGT
TGTTTCCGGG
TGTACAATAT
GGACTTCCTC
TTTTCTGGCA
ACCAAACCCA
TACATCGGGA
TTCCTATAAT
ACCTTCGTTG
GTCTCCCTAA
CATGTAGGTG
GCGGAGGGGA
GATATACAAT
AGAACAGATA
CCAGACAAGA
CATAATGGGC
TAAACAAGAC
TACACCAATT
ACACTGCCTC
ATTGATGGTG
GTACATAACG
AACTAATACT
GTAGCCCTAG
ACTTGATAGC
CATCATCATA
TCGAAGTTTC
ACTACCCTTT
TTCCATTTGC
CATCTATTGA
AGTAATAATA
GGCGCATGCA
ACTTCTTTTC
TTTTTTTTTC
TTTTCTCTCT
CCCCCGTTGT
TGTCTCACCA
TATCCGCAAT
GACAAAAAAA
ATGATGGAAG
ACACTAAAGG
AAAAAATTAA
CGACAAAGAC
AGCACCAACA
GATGTCGTTG
TTCCAGAGCT
GATGAGGGGT
ATCTCGAAGC
ACACGAAACT
TTTTCCTTCC
TTCATTCACG
CACACTACTC
TCTAATGAGC
AACGGTATAC
GGCCTTCCTT
CCAGTTACTT
GAATTTGAAA
TAAAAAAAAG
TTTGCTGTCT
TGCTATCAAG
TATAAATAGA
CCTGCAATTA
TTAATCTTTT
GTTTCCTCGT
CATTGTTCTC
GTTCCCTTTC
TTCCTTGTTT
CTTTTTCTGC
ACAATATTTC
AAGCTATACC
AAGCATACAA
TCAACTCCAA
GCTTTGCAAA
GATGGATAAA
GCGGAATTAA
TTCCCGAGCC
TCCAAAAAAG
AAGAGAAAGG
TCGAATTGGG
TACCGCCGCC
AATTTTAATC
AAAGTGGGAA
TATTGCTGAT
AGCTCATTGT
CCTTCACTTT
CACTAACAGT
AGCAACGGTC
CGAACCTCAT
AACAACTCAA
ACAAATTCTC
AAGCGCTTTC
ACAACCAATT
GCCTCCTCTA
ACGTTCATGA
TAACTTCATG
AATAATGAAA
TCACGGCTAG
TAAAATTGAT
GATGGTAATA
ATTCAAAACC
ACTGTCACCT
GGTTGGACGG
ACCAAACTGC
GTATAACGCG
TTTGGAATCA
CTACAGGGAT
GTTTAATACC
ACTACAATGG
ATGATGTATA
TAACTATCTA
TTCGATGATG
AAGATACCCC
ACCAAACCCA
AAAAAAGAGA
TCTTTAATAC
GACTCACTAT
AGGGCGAGCG
CCGCCATGGA
GTACCCATAC
GACGTACCAG
ATTACGCTCA
TATGGCCATG
GAGGCCAGTG
AATTCATGCC
TGTGGCCGGC
TCGGAGCTGC
CGCGCCGGCC
CTTGCCCCCC
GCCGCACAGG
AGCGGGACGC
CGAGCCGCGT
CCGCCGCACG
GGGAGCTGCA
GTACCTGGGG
CAGATCCAAC
ACATCCTCCG
CTGCGGCGTC
AGGAAGGACG
ACCGCACGGG
CACCGGCACC
CTGTCGGTAT
TCGGCATGCA
GGCGCGCTAC
AGCCTGAGAG
ATGAATTCCC
TCTGCTGACA
ACCAAACGTG
TGTTCTGGAA
GGGTGTTTTG
GAGGAGTTGC
TGTGGTTTAT
CAAGGGATCC
ACAAATGCTA
AAGAGCTGTC
TTCCAAGGGA
GTGAAAATCT
GGGATGCCAA
TGGATCCCGA
GACTTTTTGG
ACAGCCTGGG
ATTCTCCACC
AGAGAAGAAG
GGGACTTGGG
CCCAGTTTAT
GGCTTCCAGT
GGAGGCATTT
TGGGGCAGAA
TACAGAGATA
TGGAATCAGA
TTATTCAGGA
CAGGGAGTTG
ACCAACTGCA
AAGAGTGATT
GACACCATCA
AAACCAACCC
TGACGACAGA
AGAATCATCA
TGTGCGCTTG
GAATCCAAGA
GATCTTCCTC
TGATGGCGCT
GCCTCCATGC
CATGCCCTCT
GCCAGTTCTA
TGTGGTGAAC
AGTGAGCTGT
CCTGCCAGCT
GTACCAGAGA
TCGGGAGACA
TGGGCCTCGG
TGTGCCTTTC
AACATCGCCA
GCTACGCCCT
GCTCACGTAC
ATGATTGCGC
ACATCACGGG
CCTGAAGCCA
GGTGACTTTA
TACACACTTT
GGGAGATGCA
CATATTTACC
TGAATCACAT
CGAGCCACTG
AAAATTCAGC
TTCAGCGAGA
ACCCAGACCT
TTCCCAAAGC
TCAGGATTCT
TCGAAAAGTT
GAGAAAATTG
ATGACTTCAA
AGCTGAAGAC
TTTCAGATTG
AAGGGTACAA
TCCGCATCCA
ACTATTAAAA
TGGAAATGGC
TGTTTAGGGA
TCCATCGAGC
TCGAGCTGCA
GATGAATCGT
AGATACTGAA
AAACCCCGCA
AGTTCACTTC
AACTGTGCAT
CGTGCACCAT
CTCAATTTCT
TTCATTTATA
CATCGTTTTG
CCTTCTTTTA
TGTAACTATA
CTCCTCTAAG
TTTCAATCTT
GGCCATGTAA
CCTCTGATCT
ATAGAATTTT
TTAAATGACT
AGAATTAATG
CCCATCTTTT
TTTTGGACCT
AAATTCTTCA
TGAAAATATA
TTACGAGGGC
TTATTCAGAA
GCTTTGGACT
TCTTCGCCAG
AGGTTTGGTC
AAGTCTCCAA
TCAAGGTTGT
CGGCTTGTCT
ACCTTGCCAG
AAATTTACGA
AAAGATGGAA
AAGGGTCAAA
TCGTTGGTAG
ATACGTTGTT
GACACTTCTA
AATAAGCGAA
TTTCTTATGA
TTTATGATTT
TTATTATTAA
ATAAGTTATA
AAAAAAATAA
GTGTATACAA
ATTTTAAAGT
GACTCTTAGG
TTTTAAAACG
AAAATTCTTA
TTCTTGAGTA
ACTCTTTCCT
GTAGGTCAGG
TTGCTTTCTC
AGGTATAGCA
TGAGGTCGCT
CTTATTGACC
ACACCTCTAC
CGGCCGGTCG
AAATTCCCCT
ACCCTATGAA
CATATTCCAT
TTTGTAATTT
CGTGTCGTTT
CTATTATGAA
TTTCATTTAT
AAAGTTTATG
TACAAATATC
ATAAAAAAAG
AGAATCTTTT
TAAGCAAGGA
TTTTCTTAAC
TTCTTCGGCG
ACAGCATCAC
CGACTTCGGT
GGTACTGTTG
GAACCACCTA
AATCACCAGT
TCTGATACCT
GCATCCAAAA
CCTTTTTAAC
TGCATCTTCA
ATGGCCTTAC
CTTCTTCAGG
CAAGTTCAAT
GACAATTTCA
ACATCATTGC
AGCAGACAAG
ATAGTGGCGA
TAGGGTTGAC
CTTATTCTTT
GGCAAATCTG
GAGCAGAACC
GTGGCATGGT
TCGTACAAAC
CAAATGCGGT
GTTCTTGTCT
GGCAAAGAGG
CCAAGGACGC
AGATGGCAAC
AAACCCAAGG
AACCTGGGAT
AACGGAGGCT
TCATCGGAGA
TGATATCACC
AAACATGTTG
CTGGTGATTA
TAATACCATT
TAGGTGGGTT
GGGTTCTTAA
CTAGGATCAT
GGCGGCAGAA
TCAATCAATT
GATGTTGAAC
CTTCAATGTA
GGAAATTCGT
TCTTGATGGT
TTCCTCCACA
GTTTTTCTCC
ATAATCTTGA
AGAGGCCAAA
ACATTAGCTT
TATCCAAGGA
CCAAATAGGC
AATGGTGGCT
CATGTTGTAG
GGCCATGAAA
GCGGCCATTC
TTGTGATTCT
TTGCACTTCT
GGAACGGTGT
ATTGTTCACT
ATCCCAAGCG
ACACCATCAC
CATCGTCTTC
CTTTCTCTTA
CCAAAGTAAA
TACCTCCCAC
TAATTCTCTG
ACAACAACGA
AGTCAGTACC
TTTAGCAAAT
TGTGGCTTGA
TTGGAGATAA
GTCTAAAAGA
GAGTCGGATG
CAAAGTTACA
TGGTCTTAAG
TTGGCGTACA
ATTGAAGTTC
TTTACGGATT
TTTAGTAAAC
CTTGTTCAGG
TCTAACACTA
CCTGTACCCC
ATTTAGGACC
ACCCACAGCA
CCTAACAAAA
CGGCATCAAC
CTTCTTGGAG
GCTTCCAGCG
CCTCATCTGG
AAGTGGGACA
CCTGTAGCGT
CGATAGCAGC
ACCACCAATT
AAATGATTTT
CGAAATCGAA
CTTGACATTG
GAACGAACAT
CAGAAATAGC
TTTAAGAACC
TTAATGGCTT
CGGCTGTGAT
TTCTTGACCA
ACGTGGTCAC
CTGGCAAAAC
GACGATCTTC
TTAGGGGCAG
ACATTAGAAT
GGTATATCCT
TGAAATATAT
ATATATATTG
CTGAAATGTA
AAAGGTAAGA
AAAGTTAGAA
AGTAAGACGA
TTGCTAACCA
CCTATTGGAA
AAAACAATAG
GTCCTTAAAT
AATATTGTCA
ACTTCAAGTA
TTGTGATGCA
AGCATTTAGT
CATGAACGCT
TCTCTATTCT
ATATGAAAAG
CCGGTTCCGG
CGCTCTCACC
TTTCCTTTTT
CTCCCAATTT
TTCAGTTGAA
AAAGGTATAT
GCGTCAGGCG
ACCTCTGAAA
TTAACAAAAA
ATTTCCAGTC
ATCGAATTTG
ATTCTGTGCG
ATAGCGCCCC
TGTGTGTTCT
CGTTATGTTG
AGGAAAAAAA
TAATGGTTGC
TAAGAGATTC
GAACTCTTGC
ATCTTACGAT
ACCTGAGTAT
TCCCACAGTT
GGGGATCTCG
ACTCTAGCTA
GAGGATCAAT
TCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
ATAACTTCGT
ATAATGTATG
CTATACGAAG
TTATTAGGTC
TGAAGAGGAG
TTTACGTCCA
GCCAAGCTAG
CTTGGCTGCA
GGTCGAGCGG
CCGCGATCCG
GAACCCTTAA
TATAACTTCG
TATAATGTAT
GCTATACGAA
GTTATCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC
TAAGAAACCA
TTATTATCAT
GACATTAACC
TATAAAAATA
GGCGTATCAC
GAGGCCCTTT
CGTCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CTGGCTTAAC
TATGCGGCAT
CAGAGCAGAT
TGTACTGAGA
GTGCACCATA
ACGCATTTAA
GCATAAACAC
GCACTATGCC
GTTCTTCTCA
TGTATATATA
TATACAGGCA
ACACGCAGAT
ATAGGTGCGA
CGTGAACAGT
GAGCTGTATG
TGCGCAGCTC
GCGTTGCATT
TTCGGAAGCG
CTCGTTTTCG
GAAACGCTTT
GAAGTTCCTA
TTCCGAAGTT
CCTATTCTCT
AGCTAGAAAG
TATAGGAACT
TCAGAGCGCT
TTTGAAAACC
AAAAGCGCTC
TGAAGACGCA
CTTTCAAAAA
ACCAAAAACG
CACCGGACTG
TAACGAGCTA
CTAAAATATT
GCGAATACCG
CTTCCACAAA
CATTGCTCAA
AAGTATCTCT
TTGCTATATA
TCTCTGTGCT
ATATCCCTAT
ATAACCTACC
CATCCACCTT
TCGCTCCTTG
AACTTGCATC
TAAACTCGAC
CTCTACATTT
TTTATGTTTA
TCTCTAGTAT
TACTCTTTAG
ACAAAAAAAT
TGTAGTAAGA
ACTATTCATA
GAGTGAATCG
AAAACAATAC
GAAAATGTAA
ACATTTCCTA
TACGTAGTAT
ATAGAGACAA
AATAGAAGAA
ACCGTTCATA
ATTTTCTGAC
CAATGAAGAA
TCATCAACGC
TATCACTTTC
TGTTCACAAA
GTATGCGCAA
TCCACATCGG
TATAGAATAT
AATCGGGGAT
GCCTTTATCT
TGAAAAAATG
CACCCGCAGC
TTCGCTAGTA
ATCAGTAAAC
GCGGGAAGTG
GAGTCAGGCT
TTTTTTATGG
AAGAGAAAAT
AGACACCAAA
GTAGCCTTCT
TCTAACCTTA
ACGGACCTAC
AGTGCAAAAA
GTTATCAAGA
GACTGCATTA
TAGAGCGCAC
AAAGGAGAAA
AAAAGTAATC
TAAGATGCTT
TGTTAGAAAA
ATAGCGCTCT
CGGGATGCAT
TTTTGTAGAA
CAAAAAAGAA
GTATAGATTC
TTTGTTGGTA
AAATAGCGCT
CTCGCGTTGC
ATTTCTGTTC
TGTAAAAATG
CAGCTCAGAT
TCTTTGTTTG
AAAAATTAGC
GCTCTCGCGT
TGCATTTTTG
TTTTACAAAA
ATGAAGCACA
GATTCTTCGT
TGGTAAAATA
GCGCTTTCGC
GTTGCATTTC
TGTTCTGTAA
AAATGCAGCT
CAGATTCTTT
GTTTGAAAAA
TTAGCGCTCT
CGCGTTGCAT
TTTTGTTCTA
CAAAATGAAG
CACAGATGCT
TCGTTGCT