名称: | pGBKT7-RRM2(human) |
---|---|
别称: | NM_001034.4;C2orf48; R2; RR2; RR2M |
基因长: | 1170bp |
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | TRP1 |
荧光标记: | |
启动子: | ADH1 |
复制子: | 2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pGBKT7 |
正向引物: | T7 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGA
TCCTTTTGTT
GTTTCCGGGT
GTACAATATG
GACTTCCTCT
TTTCTGGCAA
CCAAACCCAT
ACATCGGGAT
TCCTATAATA
CCTTCGTTGG
TCTCCCTAAC
ATGTAGGTGG
CGGAGGGGAG
ATATACAATA
GAACAGATAC
CAGACAAGAC
ATAATGGGCT
AAACAAGACT
ACACCAATTA
CACTGCCTCA
TTGATGGTGG
TACATAACGA
ACTAATACTG
TAGCCCTAGA
CTTGATAGCC
ATCATCATAT
CGAAGTTTCA
CTACCCTTTT
TCCATTTGCC
ATCTATTGAA
GTAATAATAG
GCGCATGCAA
CTTCTTTTCT
TTTTTTTTCT
TTTCTCTCTC
CCCCGTTGTT
GTCTCACCAT
ATCCGCAATG
ACAAAAAAAT
GATGGAAGAC
ACTAAAGGAA
AAAATTAACG
ACAAAGACAG
CACCAACAGA
TGTCGTTGTT
CCAGAGCTGA
TGAGGGGTAT
CTCGAAGCAC
ACGAAACTTT
TTCCTTCCTT
CATTCACGCA
CACTACTCTC
TAATGAGCAA
CGGTATACGG
CCTTCCTTCC
AGTTACTTGA
ATTTGAAATA
AAAAAAAGTT
TGCTGTCTTG
CTATCAAGTA
TAAATAGACC
TGCAATTATT
AATCTTTTGT
TTCCTCGTCA
TTGTTCTCGT
TCCCTTTCTT
CCTTGTTTCT
TTTTCTGCAC
AATATTTCAA
GCTATACCAA
GCATACAATC
AACTCCAAGC
TTGAAGCAAG
CCTCCTGAAA
GATGAAGCTA
CTGTCTTCTA
TCGAACAAGC
ATGCGATATT
TGCCGACTTA
AAAAGCTCAA
GTGCTCCAAA
GAAAAACCGA
AGTGCGCCAA
GTGTCTGAAG
AACAACTGGG
AGTGTCGCTA
CTCTCCCAAA
ACCAAAAGGT
CTCCGCTGAC
TAGGGCACAT
CTGACAGAAG
TGGAATCAAG
GCTAGAAAGA
CTGGAACAGC
TATTTCTACT
GATTTTTCCT
CGAGAAGACC
TTGACATGAT
TTTGAAAATG
GATTCTTTAC
AGGATATAAA
AGCATTGTTA
ACAGGATTAT
TTGTACAAGA
TAATGTGAAT
AAAGATGCCG
TCACAGATAG
ATTGGCTTCA
GTGGAGACTG
ATATGCCTCT
AACATTGAGA
CAGCATAGAA
TAAGTGCGAC
ATCATCATCG
GAAGAGAGTA
GTAACAAAGG
TCAAAGACAG
TTGACTGTAT
CGCCGGAATT
TGTAATACGA
CTCACTATAG
GGCGAGCCGC
CATCATGGAG
GAGCAGAAGC
TGATCTCAGA
GGAGGACCTG
CATATGGCCA
TGGAGGCCGA
ATTCATGCTC
TCCCTCCGTG
TCCCGCTCGC
GCCCATCACG
GACCCGCAGC
AGCTGCAGCT
CTCGCCGCTG
AAGGGGCTCA
GCTTGGTCGA
CAAGGAGAAC
ACGCCGCCGG
CCCTGAGCGG
GACCCGCGTC
CTGGCCAGCA
AGACCGCGAG
GAGGATCTTC
CAGGAGCCCA
CGGAGCCGAA
AACTAAAGCA
GCTGCCCCCG
GCGTGGAGGA
TGAGCCGCTG
CTGAGAGAAA
ACCCCCGCCG
CTTTGTCATC
TTCCCCATCG
AGTACCATGA
TATCTGGCAG
ATGTATAAGA
AGGCAGAGGC
TTCCTTTTGG
ACCGCCGAGG
AGGTGGACCT
CTCCAAGGAC
ATTCAGCACT
GGGAATCCCT
GAAACCCGAG
GAGAGATATT
TTATATCCCA
TGTTCTGGCT
TTCTTTGCAG
CAAGCGATGG
CATAGTAAAT
GAAAACTTGG
TGGAGCGATT
TAGCCAAGAA
GTTCAGATTA
CAGAAGCCCG
CTGTTTCTAT
GGCTTCCAAA
TTGCCATGGA
AAACATACAT
TCTGAAATGT
ATAGTCTTCT
TATTGACACT
TACATAAAAG
ATCCCAAAGA
AAGGGAATTT
CTCTTCAATG
CCATTGAAAC
GATGCCTTGT
GTCAAGAAGA
AGGCAGACTG
GGCCTTGCGC
TGGATTGGGG
ACAAAGAGGC
TACCTATGGT
GAACGTGTTG
TAGCCTTTGC
TGCAGTGGAA
GGCATTTTCT
TTTCCGGTTC
TTTTGCGTCG
ATATTCTGGC
TCAAGAAACG
AGGACTGATG
CCTGGCCTCA
CATTTTCTAA
TGAACTTATT
AGCAGAGATG
AGGGTTTACA
CTGTGATTTT
GCTTGCCTGA
TGTTCAAACA
CCTGGTACAC
AAACCATCGG
AGGAGAGAGT
AAGAGAAATA
ATTATCAATG
CTGTTCGGAT
AGAACAGGAG
TTCCTCACTG
AGGCCTTGCC
TGTGAAGCTC
ATTGGGATGA
ATTGCACTCT
AATGAAGCAA
TACATTGAGT
TTGTGGCAGA
CAGACTTATG
CTGGAACTGG
GTTTTAGCAA
GGTTTTCAGA
GTAGAGAACC
CATTTGACTT
TATGGAGAAT
ATTTCACTGG
AAGGAAAGAC
TAACTTCTTT
GAGAAGAGAG
TAGGCGAGTA
TCAGAGGATG
GGAGTGATGT
CAAGTCCAAC
AGAGAATTCT
TTTACCTTGG
ATGCTGACTT
CTAAGGATCC
GTCGACCTGC
AGCGGCCGCA
TAACTAGCAT
AACCCCTTGG
GGCCTCTAAA
CGGGTCTTGA
GGGGTTTTTT
GCGCGCTTGC
AGCCAAGCTA
ATTCCGGGCG
AATTTCTTAT
GATTTATGAT
TTTTATTATT
AAATAAGTTA
TAAAAAAAAT
AAGTGTATAC
AAATTTTAAA
GTGACTCTTA
GGTTTTAAAA
CGAAAATTCT
TATTCTTGAG
TAACTCTTTC
CTGTAGGTCA
GGTTGCTTTC
TCAGGTATAG
CATGAGGTCG
CTCTTATTGA
CCACACCTCT
ACCGGCATGC
AAGCTTGGCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAACCTGA
GGCTATGGCA
GGGCCTGCCG
CCCCGACGTT
GGCTGCGAGC
CCTGGGCCTT
CACCCGAACT
TGGGGGGTGG
GGTGGGGAAA
AGGAAGAAAC
GCGGGCGTAT
TGGCCCCAAT
GGGGTCTCGG
TGGGGTATCG
ACAGAGTGCC
AGCCCTGGGA
CCGAACCCCG
CGTTTATGAA
CAAACGACCC
AACACCGTGC
GTTTTATTCT
GTCTTTTTAT
TGCCGTCATA
GCGCGGGTTC
CTTCCGGTAT
TGTCTCCTTC
CGTGTTTCAG
TTAGCCTCCC
CCTAGGGTGG
GCGAAGAACT
CCAGCATGAG
ATCCCCGCGC
TGGAGGATCA
TCCAGCCGGC
GTCCCGGAAA
ACGATTCCGA
AGCCCAACCT
TTCATAGAAG
GCGGCGGTGG
AATCGAAATC
TCGTGATGGC
AGGTTGGGCG
TCGCTTGGTC
GGTCATTTCG
AACCCCAGAG
TCCCGCTCAG
AAGAACTCGT
CAAGAAGGCG
ATAGAAGGCG
ATGCGCTGCG
AATCGGGAGC
GGCGATACCG
TAAAGCACGA
GGAAGCGGTC
AGCCCATTCG
CCGCCAAGCT
CTTCAGCAAT
ATCACGGGTA
GCCAACGCTA
TGTCCTGATA
GCGGTCCGCC
ACACCCAGCC
GGCCACAGTC
GATGAATCCA
GAAAAGCGGC
CATTTTCCAC
CATGATATTC
GGCAAGCAGG
CATCGCCATG
AGTCACGACG
AGATCCTCGC
CGTCGGGCAT
GCTCGCCTTG
AGCCTGGCGA
ACAGTTCGGC
TGGCGCGAGC
CCCTGATGCT
CTTCGTCCAG
ATCATCCTGA
TCGACAAGAC
CGGCTTCCAT
CCGAGTACGT
GCTCGCTCGA
TGCGATGTTT
CGCTTGGTGG
TCGAATGGGC
AGGTAGCCGG
ATCAAGCGTA
TGCAGCCGCC
GCATTGCATC
AGCCATGATG
GATACTTTCT
CGGCAGGAGC
AAGGTGAGAT
GACAGGAGAT
CCTGCCCCGG
CACTTCGCCC
AATAGCAGCC
AGTCCCTTCC
CGCTTCAGTG
ACAACGTCGA
GCACAGCTGC
GCAAGGAACG
CCCGTCGTGG
CCAGCCACGA
TAGCCGCGCT
GCCTCGTCTT
GCAGTTCATT
CAGGGCACCG
GACAGGTCGG
TCTTGACAAA
AAGAACCGGG
CGCCCCTGCG
CTGACAGCCG
GAACACGGCG
GCATCAGAGC
AGCCGATTGT
CTGTTGTGCC
CAGTCATAGC
CGAATAGCCT
CTCCACCCAA
GCGGCCGGAG
AACCTGCGTG
CAATCCATCT
TGTTCAATCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGAACGAAGC
ATCTGTGCTT
CATTTTGTAG
AACAAAAATG
CAACGCGAGA
GCGCTAATTT
TTCAAACAAA
GAATCTGAGC
TGCATTTTTA
CAGAACAGAA
ATGCAACGCG
AAAGCGCTAT
TTTACCAACG
AAGAATCTGT
GCTTCATTTT
TGTAAAACAA
AAATGCAACG
CGAGAGCGCT
AATTTTTCAA
ACAAAGAATC
TGAGCTGCAT
TTTTACAGAA
CAGAAATGCA
ACGCGAGAGC
GCTATTTTAC
CAACAAAGAA
TCTATACTTC
TTTTTTGTTC
TACAAAAATG
CATCCCGAGA
GCGCTATTTT
TCTAACAAAG
CATCTTAGAT
TACTTTTTTT
CTCCTTTGTG
CGCTCTATAA
TGCAGTCTCT
TGATAACTTT
TTGCACTGTA
GGTCCGTTAA
GGTTAGAAGA
AGGCTACTTT
GGTGTCTATT
TTCTCTTCCA
TAAAAAAAGC
CTGACTCCAC
TTCCCGCGTT
TACTGATTAC
TAGCGAAGCT
GCGGGTGCAT
TTTTTCAAGA
TAAAGGCATC
CCCGATTATA
TTCTATACCG
ATGTGGATTG
CGCATACTTT
GTGAACAGAA
AGTGATAGCG
TTGATGATTC
TTCATTGGTC
AGAAAATTAT
GAACGGTTTC
TTCTATTTTG
TCTCTATATA
CTACGTATAG
GAAATGTTTA
CATTTTCGTA
TTGTTTTCGA
TTCACTCTAT
GAATAGTTCT
TACTACAATT
TTTTTGTCTA
AAGAGTAATA
CTAGAGATAA
ACATAAAAAA
TGTAGAGGTC
GAGTTTAGAT
GCAAGTTCAA
GGAGCGAAAG
GTGGATGGGT
AGGTTATATA
GGGATATAGC
ACAGAGATAT
ATAGCAAAGA
GATACTTTTG
AGCAATGTTT
GTGGAAGCGG
TATTCGCAAT
ATTTTAGTAG
CTCGTTACAG
TCCGGTGCGT
TTTTGGTTTT
TTGAAAGTGC
GTCTTCAGAG
CGCTTTTGGT
TTTCAAAAGC
GCTCTGAAGT
TCCTATACTT
TCTAGAGAAT
AGGAACTTCG
GAATAGGAAC
TTCAAAGCGT
TTCCGAAAAC
GAGCGCTTCC
GAAAATGCAA
CGCGAGCTGC
GCACATACAG
CTCACTGTTC
ACGTCGCACC
TATATCTGCG
TGTTGCCTGT
ATATATATAT
ACATGAGAAG
AACGGCATAG
TGCGTGTTTA
TGCTTAAATG
CGTACTTATA
TGCGTCTATT
TATGTAGGAT
GAAAGGTAGT
CTAGTACCTC
CTGTGATATT
ATCCCATTCC
ATGCGGGGTA
TCGTATGCTT
CCTTCAGCAC
TACCCTTTAG
CTGTTCTATA
TGCTGCCACT
CCTCAATTGG
ATTAGTCTCA
TCCTTCAATG
CTATCATTTC
CTTTGATATT
GGATCATACT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCCCTTTC
GTCTCGCGCG
TTTCGGTGAT
GACGGTGAAA
ACCTCTGACA
CATGCAGCTC
CCGGAGACGG
TCACAGCTTG
TCTGTAAGCG
GATGCCGGGA
GCAGACAAGC
CCGTCAGGGC
GCGTCAGCGG
GTGTTGGCGG
GTGTCGGGGC
TGGCTTAACT
ATGCGGCATC
AGAGCAGATT
GTACTGAGAG
TGCACCATAG
ATCAACGACA
TTACTATATA
TATAATATAG
GAAGCATTTA
ATAGAACAGC
ATCGTAATAT
ATGTGTACTT
TGCAGTTATG
ACGCCAGATG
GCAGTAGTGG
AAGATATTCT
TTATTGAAAA
ATAGCTTGTC
ACCTTACGTA
CAATCTTGAT
CCGGAGCTTT
TCTTTTTTTG
CCGATTAAGA
ATTAATTCGG
TCGAAAAAAG
AAAAGGAGAG
GGCCAAGAGG
GAGGGCATTG
GTGACTATTG
AGCACGTGAG
TATACGTGAT
TAAGCACACA
AAGGCAGCTT
GGAGTATGTC
TGTTATTAAT
TTCACAGGTA
GTTCTGGTCC
ATTGGTGAAA
GTTTGCGGCT
TGCAGAGCAC
AGAGGCCGCA
GAATGTGCTC
TAGATTCCGA
TGCTGACTTG
CTGGGTATTA
TATGTGTGCC
CAATAGAAAG
AGAACAATTG
ACCCGGTTAT
TGCAAGGAAA
ATTTCAAGTC
TTGTAAAAGC
ATATAAAAAT
AGTTCAGGCA
CTCCGAAATA
CTTGGTTGGC
GTGTTTCGTA
ATCAACCTAA
GGAGGATGTT
TTGGCTCTGG
TCAATGATTA
CGGCATTGAT
ATCGTCCAAC
TGCATGGAGA
TGAGTCGTGG
CAAGAATACC
AAGAGTTCCT
CGGTTTGCCA
GTTATTAAAA
GACTCGTATT
TCCAAAAGAC
TGCAACATAC
TACTCAGTGC
AGCTTCACAG
AAACCTCATT
CGTTTATTCC
CTTGTTTGAT
TCAGAAGCAG
GTGGGACAGG
TGAACTTTTG
GATTGGAACT
CGATTTCTGA
CTGGGTTGGA
AGGCAAGAGA
GCCCCGAAAG
CTTACATTTT
ATGTTAGCTG
GTGGACTGAC
GCCAGAAAAT
GTTGGTGATG
CGCTTAGATT
AAATGGCGTT
ATTGGTGTTG
ATGTAAGCGG
AGGTGTGGAG
ACAAATGGTG
TAAAAGACTC
TAACAAAATA
GCAAATTTCG
TCAAAAATGC
TAAGAAATAG
GTTATTACTG
AGTAGTATTT
ATTTAAGTAT
TGTTTGTGCA
CTTGCCGATC
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGAAATTGTA
AGCGTTAATA
TTTTGTTAAA
ATTCGCGTTA
AATTTTTGTT
AAATCAGCTC
ATTTTTTAAC
CAATAGGCCG
AAATCGGCAA
AATCCCTTAT
AAATCAAAAG
AATAGACCGA
GATAGGGTTG
AGTGTTGTTC
CAGTTTGGAA
CAAGAGTCCA
CTATTAAAGA
ACGTGGACTC
CAACGTCAAA
GGGCGAAAAA
CCGTCTATCA
GGGCGATGGC
CCACTACGTG
AACCATCACC
CTAATCAAGT
TTTTTGGGGT
CGAGGTGCCG
TAAAGCACTA
AATCGGAACC
CTAAAGGGAG
CCCCCGATTT
AGAGCTTGAC
GGGGAAAGCC
GGCGAACGTG
GCGAGAAAGG
AAGGGAAGAA
AGCGAAAGGA
GCGGGCGCTA
GGGCGCTGGC
AAGTGTAGCG
GTCACGCTGC
GCGTAACCAC
CACACCCGCC
GCGCTTAATG
CGCCGCTACA
GGGCGCGTCC
ATTCGCCATT
CAGGCTGCGC
AACTGTTGGG
AAGGGCGAT