名称: | pLV2-EF1a-GUCY2C(Cynomolgus-opt)-EGFP-Puro |
---|---|
别称: | https://www.uniprot.org/uniprotkb/G7PJX5/entry |
基因长: | 3219bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 食蟹猴 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV2-EF1a-MCS-3×FLAG-Puro |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATGAGTAA
TTCATACAAA
AGGACTCGCC
CCTGCCTTGG
GGAATCCCAG
GGACCGTCGT
TAAACTCCCA
CTAACGTAGA
ACCCAGAGAT
CGCTGCGTTC
CCGCCCCCTC
ACCCGCCCGC
TCTCGTCATC
ACTGAGGTGG
AGAAGAGCAT
GCGTGAGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACGCCCCT
GGCTGCAGTA
CGTGATTCTT
GATCCCGAGC
TTCGGGTTGG
AAGTGGGTGG
GAGAGTTCGA
GGCCTTGCGC
TTAAGGAGCC
CCTTCGCCTC
GTGCTTGAGT
TGAGGCCTGG
CTTGGGCGCT
GGGGCCGCCG
CGTGCGAATC
TGGTGGCACC
TTCGCGCCTG
TCTCGCTGCT
TTCGATAAGT
CTCTAGCCAT
TTAAAATTTT
TGATGACCTG
CTGCGACGCT
TTTTTTCTGG
CAAGATAGTC
TTGTAAATGC
GGGCCAAGAT
CTGCACACTG
GTATTTCGGT
TTTTGGGGCC
GCGGGCGGCG
ACGGGGCCCG
TGCGTCCCAG
CGCACATGTT
CGGCGAGGCG
GGGCCTGCGA
GCGCGGCCAC
CGAGAATCGG
ACGGGGGTAG
TCTCAAGCTG
GCCGGCCTGC
TCTGGTGCCT
GGCCTCGCGC
CGCCGTGTAT
CGCCCCGCCC
TGGGCGGCAA
GGCTGGCCCG
GTCGGCACCA
GTTGCGTGAG
CGGAAAGATG
GCCGCTTCCC
GGCCCTGCTG
CAGGGAGCTC
AAAATGGAGG
ACGCGGCGCT
CGGGAGAGCG
GGCGGGTGAG
TCACCCACAC
AAAGGAAAAG
GGCCTTTCCG
TCCTCAGCCG
TCGCTTCATG
TGACTCCACG
GAGTACCGGG
CGCCGTCCAG
GCACCTCGAT
TAGTTCTCGA
GCTTTTGGAG
TACGTCGTCT
TTAGGTTGGG
GGGAGGGGTT
TTATGCGATG
GAGTTTCCCC
ACACTGAGTG
GGTGGAGACT
GAAGTTAGGC
CAGCTTGGCA
CTTGATGTAA
TTCTCCTTGG
AATTTGCCCT
TTTTGAGTTT
GGATCTTGGT
TCATTCTCAA
GCCTCAGACA
GTGGTTCAAA
GTTTTTTTCT
TCCATTTCAG
GTGTCGTGAG
GAATTAGCTT
GGTACGAATT
CGCCACCATG
AAGACCCTGC
TGCTGGACCT
GGTTCTGTGG
TCCCTGCTGT
TTCAGCCCGA
GTGGCTGTAC
CTGACAAGCC
AGGTGTCCCA
GAACTGCCAC
AACGGCAGCT
ACGAGATCAG
CGTGCTGATG
ATGGACAACA
GCGCCTTCGC
CGAGCCTCTG
GAAAACGTGG
AAGATGCCGT
GAACGAAGGC
CTGGAAATCG
TGCGAGGCAG
ACTGCAGAAT
GCCGGCCTGA
ACGTGACAGT
GAACGCCAGC
TTCATGTACA
GCGACGGCCT
GATCCACAAC
AGCGGCGACT
GTAGAAGCAG
CACCTGTGAA
GGACTGGACC
TGCTGCGGAA
GATCAGCAAC
GCCAAGAGAA
TGGGCTGTGT
GCTGATGGGC
CCTAGCTGCA
CCTACAGCAC
CTTCCAGATG
TACCTGGACA
CCGAGCTGAG
CTACCCCATG
ATCAGCGCCG
GAAGCTTTGG
CCTGAGCTGC
GACTACAAAG
AGACACTGAC
CCGGCTGATG
AGCCCCGCCA
GAAAGCTGAC
CTACTTCCTG
GTCAACTTTT
GGAAAACGAA
CGACCTGCCG
TTCAAGACCT
ACAGCTGGTC
CACCAGCTAC
GTGTACAAGA
ACGGCACCGA
GAGCGAGGAC
TGCTTCTGGT
ATCTGAACGC
CCTGGAAGCC
TCCGTGTCCT
ACTTTAGCCA
CGAGCTGTCC
TTCAAGCTGG
TGCTGCGGCA
GGACAAAGAG
TTCCAGGACA
TCCTGATGGA
CCACAACCGG
AAGTCCAACG
TGATCGTGAT
GTGCGGCGAC
CCCGAGTTCC
TGTACAAGCT
GAAAGGCGAT
AGAGCCGTGG
CCGAGGACAT
CGTGATCATC
CTGGTGGATC
TGTTCAACGA
CCAGTACTTC
GAGGACAATG
TGACAGCCCC
TGACTACATG
AAGAACGTGC
TGGTCCTGAC
AAGAAGCCCC
GGCAACAGCC
TGCTGAACAG
CAGCTTCAGC
AGAAATCTGA
GCCCCACCAA
GCGGGATTTC
GCCCTGGCCT
ATCTGAATGG
CATCCTGCTG
TTCGGCCACA
TGCTGAAAAC
ATTCCTCGAG
AACGGCGAGA
ACATCACGAC
CCCTAAGTTC
GCCCACGCCT
TCCGGAATCT
GACCTTCGAG
GGCTATGACG
GCCCCGTGAC
ACTTGATGAC
TGGGGCGACG
TGGACAGCAC
AATGGTGCTG
CTGTACACCA
GCGTGGACAC
CAAAAAGTAC
AAAGTGCTGC
TGACATACGA
CACCCACGTG
AACCAGACAA
ACCCCGTGGA
CATGAGCCCT
ACCTTCACCT
GGAAGAACAG
CAAGCTGCCC
AACGACATCA
CCGACAGAGG
CCCTCAGATC
CTGATGATCG
CCGTGTTCAC
ACTGACAGGC
GCCGTGGTTC
TGCTGCTGCT
TGTTGCTCTG
CTGATGCTGA
GGAAGTACAA
GAAGGACTAC
GAGCTGAGGC
AGAAGAAGTG
GTCCCACATT
CCTCCAGAGA
ACATCTTCCC
ACTGGAAACA
AACGAGACTA
ACCACGTGTC
CCTGAAGATC
GACGACGACA
AGCGGAGAGA
CACCATCCAG
AGACTGCGGC
AGTGCAAATA
CGACAAGAAA
CGCGTGATCC
TGAAGGACCT
GAAGCACAAC
GACGGCAACT
TCACCGAGAA
GCAGAAGATC
GAGCTGAACA
AGCTGCTGCA
GATCGACTAC
TACAACCTGA
CCAAGTTCTA
CGGCACCGTG
AAGCTGGACA
CCATGATCTT
CGGCGTGATC
GAGTACTGCG
AGCGGGGCTC
TCTGAGAGAG
GTGCTGAACG
ACACCATCAG
CTACCCCGAT
GGCACCTTCA
TGGACTGGGA
GTTCAAGATC
TCCGTGCTGT
ACGATATCGC
CAAGGGCATG
AGCTACCTGC
ACAGCAGCAA
GACAGAGGTG
CACGGCAGAC
TGAAGTCCAC
CAACTGCGTG
GTGGATAGCC
GGATGGTGGT
CAAGATCACC
GACTTCGGCT
GCAACAGCAT
CCTGCCTCCA
AAGAAAGACC
TGTGGACAGC
CCCAGAGCAC
CTGAGACAGG
CCAATGTGTC
TCAGAAAGGC
GACGTGTACT
CCTACGGCAT
CATTGCCCAA
GAGATCATCC
TCCGGAAAGA
GACATTCTAC
ACCAGCAGCT
GCCGGGACAG
AAACGAGAAG
ATCTTTCGCG
TGGAAAACTC
CAACGGCATG
AAGCCCTTCA
GACCCGACCT
GTTCCTGGAA
ACCGCCGAAG
AGAAAGAACT
GGAAGTGTAC
CTGCTGGTCA
AGAGCTGCTG
GGAAGAAGAT
CCCGAGAAGC
GGCCCGACTT
CAAGAAGATT
GAGACAACCC
TGGCCAAGAT
CTTCGGACTG
TTCCACGATC
AGAAAAACGA
GAGCTACATG
GATACCCTGA
TCCGGCGGCT
GCAGCTGTAC
TCCAGAAACC
TGGAACACCT
GGTGGAAGAG
AGAACCCAGC
TGTACAAGGC
CGAGAGAGAC
AGAGCCGACC
GGCTGAACTT
TATGCTGCTG
CCCAGACTGG
TCGTGAAGTC
CCTGAAAGAG
AAGGGCTTTG
TCGAGCCCGA
GCTGTACGAG
GAAGTGACCA
TCTACTTCAG
CGACATCGTG
GGCTTCACCA
CCATCTGCAA
GTACAGCACC
CCTATGGAAG
TGGTGGACAT
GCTCAACGAT
ATCTACAAGA
GCTTCGACCA
CATCGTGGAC
CACCACGACG
TCTACAAGGT
GGAAACAATC
GGCGACGCCT
ACATGGTGGC
CTCTGGCCTG
CCTAAGAGAA
ACGGCAACAG
ACACGCCATC
GACATTGCCA
AGATGGCCCT
GGAAATTCTG
AGCTTCATGG
GCACCTTCGA
GCTGGAACAT
CTGCCCGGAC
TGCCCATCTG
GATCAGAATC
GGAGTGCACA
GCGGACCTTG
TGCCGCTGGT
GTTGTGGGCA
TCAAGATGCC
TCGGTACTGC
CTGTTCGGCG
ACACCGTGAA
TACCGCCAGC
AGAATGGAAA
GCACCGGCCT
GCCTCTGCGG
ATCCATGTGT
CTGGAAGCAC
AATCGCCATC
CTGAAGCGGA
CCGAGTGCCA
GTTTCTGTAC
GAAGTGCGGG
GCGAGACATA
CCTGAAAGGC
AGAGGCAACG
AGACAACCTA
CTGGCTGACA
GGCATGAAGG
ATCAGAAGTT
CAACCTGCCT
ACACCTCCTA
CCGTGGAAAA
TCAGCAGCGA
CTGCAGGCCG
AGTTCTCCGA
CATGATCGCC
AATAGCCTGC
AGAAGAGACA
GGCTGCCGGC
ATCAGAAGCC
AAAAGCCTAG
AAGAGTGGCC
AGCTATAAGA
AGGGCACCCT
GGAATACCTG
CAGCTGAACA
CCACCGACAA
AGAGAGCACC
TACTTCGGCG
GTGGAGGAAG
CGGCGGAGGT
GGATCTATGG
TGAGCAAGGG
CGAGGAGCTG
TTCACCGGGG
TGGTGCCCAT
CCTGGTCGAG
CTGGACGGCG
ACGTAAACGG
CCACAAGTTC
AGCGTGTCCG
GCGAGGGCGA
GGGCGATGCC
ACCTACGGCA
AGCTGACCCT
GAAGTTCATC
TGCACCACCG
GCAAGCTGCC
CGTGCCCTGG
CCCACCCTCG
TGACCACCCT
GACCTACGGC
GTGCAGTGCT
TCAGCCGCTA
CCCCGACCAC
ATGAAGCAGC
ACGACTTCTT
CAAGTCCGCC
ATGCCCGAAG
GCTACGTCCA
GGAGCGCACC
ATCTTCTTCA
AGGACGACGG
CAACTACAAG
ACCCGCGCCG
AGGTGAAGTT
CGAGGGCGAC
ACCCTGGTGA
ACCGCATCGA
GCTGAAGGGC
ATCGACTTCA
AGGAGGACGG
CAACATCCTG
GGGCACAAGC
TGGAGTACAA
CTACAACAGC
CACAACGTCT
ATATCATGGC
CGACAAGCAG
AAGAACGGCA
TCAAGGTGAA
CTTCAAGATC
CGCCACAACA
TCGAGGACGG
CAGCGTGCAG
CTCGCCGACC
ACTACCAGCA
GAACACCCCC
ATCGGCGACG
GCCCCGTGCT
GCTGCCCGAC
AACCACTACC
TGAGCACCCA
GTCCGCCCTG
AGCAAAGACC
CCAACGAGAA
GCGCGATCAC
ATGGTCCTGC
TGGAGTTCGT
GACCGCCGCC
GGGATCACTC
TCGGCATGGA
CGAGCTGTAC
AAGTAGGCCT
GCGATATCCA
GCGGATCCCG
CCCCTCTCCC
TCCCCCCCCC
CTAACGTTAC
TGGCCGAAGC
CGCTTGGAAT
AAGGCCGGTG
TGCGTTTGTC
TATATGTTAT
TTTCCACCAT
ATTGCCGTCT
TTTGGCAATG
TGAGGGCCCG
GAAACCTGGC
CCTGTCTTCT
TGACGAGCAT
TCCTAGGGGT
CTTTCCCCTC
TCGCCAAAGG
AATGCAAGGT
CTGTTGAATG
TCGTGAAGGA
AGCAGTTCCT
CTGGAAGCTT
CTTGAAGACA
AACAACGTCT
GTAGCGACCC
TTTGCAGGCA
GCGGAACCCC
CCACCTGGCG
ACAGGTGCCT
CTGCGGCCAA
AAGCCACGTG
TATAAGATAC
ACCTGCAAAG
GCGGCACAAC
CCCAGTGCCA
CGTTGTGAGT
TGGATAGTTG
TGGAAAGAGT
CAAATGGCTC
TCCTCAAGCG
TATTCAACAA
GGGGCTGAAG
GATGCCCAGA
AGGTACCCCA
TTGTATGGGA
TCTGATCTGG
GGCCTCGGTG
CACATGCTTT
ACATGTGTTT
AGTCGAGGTT
AAAAAAACGT
CTAGGCCCCC
CGAACCACGG
GGACGTGGTT
TTCCTTTGAA
AAACACGATG
ATAAGCTTGC
CACAACCCAC
AAGGAGACGA
CCTTCCATGA
CCGAGTACAA
GCCCACGGTG
CGCCTCGCCA
CCCGCGACGA
CGTCCCCCGG
GCCGTACGCA
CCCTCGCCGC
CGCGTTCGCC
GACTACCCCG
CCACGCGCCA
CACCGTCGAC
CCGGACCGCC
ACATCGAGCG
GGTCACCGAG
CTGCAAGAAC
TCTTCCTCAC
GCGCGTCGGG
CTCGACATCG
GCAAGGTGTG
GGTCGCGGAC
GACGGCGCCG
CGGTGGCGGT
CTGGACCACG
CCGGAGAGCG
TCGAAGCGGG
GGCGGTGTTC
GCCGAGATCG
GCCCGCGCAT
GGCCGAGTTG
AGCGGTTCCC
GGCTGGCCGC
GCAGCAACAG
ATGGAAGGCC
TCCTGGCGCC
GCACCGGCCC
AAGGAGCCCG
CGTGGTTCCT
GGCCACCGTC
GGCGTCTCGC
CCGACCACCA
GGGCAAGGGT
CTGGGCAGCG
CCGTCGTGCT
CCCCGGAGTG
GAGGCGGCCG
AGCGCGCCGG
GGTGCCCGCC
TTCCTGGAGA
CCTCCGCGCC
CCGCAACCTC
CCCTTCTACG
AGCGGCTCGG
CTTCACCGTC
ACCGCCGACG
TCGAGGTGCC
CGAAGGACCG
CGCACCTGGT
GCATGACCCG
CAAGCCCGGT
GCCTAGACGC
GTCTGGAACA
ATCAACCTCT
GGATTACAAA
ATTTGTGAAA
GATTGACTGG
TATTCTTAAC
TATGTTGCTC
CTTTTACGCT
ATGTGGATAC
GCTGCTTTAA
TGCCTTTGTA
TCATGCTATT
GCTTCCCGTA
TGGCTTTCAT
TTTCTCCTCC
TTGTATAAAT
CCTGGTTGCT
GTCTCTTTAT
GAGGAGTTGT
GGCCCGTTGT
CAGGCAACGT
GGCGTGGTGT
GCACTGTGTT
TGCTGACGCA
ACCCCCACTG
GTTGGGGCAT
TGCCACCACC
TGTCAGCTCC
TTTCCGGGAC
TTTCGCTTTC
CCCCTCCCTA
TTGCCACGGC
GGAACTCATC
GCCGCCTGCC
TTGCCCGCTG
CTGGACAGGG
GCTCGGCTGT
TGGGCACTGA
CAATTCCGTG
GTGTTGTCGG
GGAAGCTGAC
GTCCTTTCCA
TGGCTGCTCG
CCTGTGTTGC
CACCTGGATT
CTGCGCGGGA
CGTCCTTCTG
CTACGTCCCT
TCGGCCCTCA
ATCCAGCGGA
CCTTCCTTCC
CGCGGCCTGC
TGCCGGCTCT
GCGGCCTCTT
CCGCGTCTTC
GCCTTCGCCC
TCAGACGAGT
CGGATCTCCC
TTTGGGCCGC
CTCCCCGCCT
GGAATTAATT
CTGCAGTCGA
GACCTAGAAA
AACATGGAGC
AATCACAAGT
AGCAATACAG
CAGCTACCAA
TGCTGATTGT
GCCTGGCTAG
AAGCACAAGA
GGAGGAGGAG
GTGGGTTTTC
CAGTCACACC
TCAGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGAGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AGACAAGATA
TCCTTGATCT
GTGGATCTAC
CACACACAAG
GCTACTTCCC
TGATTAGCAG
AACTACACAC
CAGGGCCAGG
GGTCAGATAT
CCACTGACCT
TTGGATGGTG
CTACAAGCTA
GTACCAGTTG
AGCCAGATAA
GGTAGAAGAG
GCCAATAAAG
GAGAGAACAC
CAGCTTGTTA
CACCCTGTGA
GCCTGCATGG
GATGGATGAC
CCGGAGAGAG
AAGTGTTAGA
GTGGAGGTTT
GACAGCCGCC
TAGCATTTCA
TCACGTGGCC
CGAGAGCTGC
ATCCGGAGTA
CTTCAAGAAC
TGCTGATATC
GAGCTTGCTA
CAAGGGACTT
TCCGCTGGGG
ACTTTCCAGG
GAGGCGTGGC
CTGGGCGGGA
CTGGGGAGTG
GCGAGCCCTC
AGATCCTGCA
TATAAGCAGC
TGCTTTTTGC
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
AGTAGTTCAT
GTCATCTTAT
TATTCAGTAT
TTATAACTTG
CAAAGAAATG
AATATCAGAG
AGTGAGAGGC
CTTGACATTG
CTAGCGTTTA
CCGTCGACCT
CTAGCTAGAG
CTTGGCGTAA
TCATGGTCAT
AGCTGTTTCC
TGTGTGAAAT
TGTTATCCGC
TCACAATTCC
ACACAACATA
CGAGCCGGAA
GCATAAAGTG
TAAAGCCTGG
GGTGCCTAAT
GAGTGAGCTA
ACTCACATTA
ATTGCGTTGC
GCTCACTGCC
CGCTTTCCAG
TCGGGAAACC
TGTCGTGCCA
GCTGCATTAA
TGAATCGGCC
AACGCGCGGG
GAGAGGCGGT
TTGCGTATTG
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGAA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
CAATGCTTAA
TCAGTGAGGC
ACCTATCTCA
GCGATCTGTC
TATTTCGTTC
ATCCATAGTT
GCCTGACTCC
CCGTCGTGTA
GATAACTACG
ATACGGGAGG
GCTTACCATC
TGGCCCCAGT
GCTGCAATGA
TACCGCGAGA
CCCACGCTCA
CCGGCTCCAG
ATTTATCAGC
AATAAACCAG
CCAGCCGGAA
GGGCCGAGCG
CAGAAGTGGT
CCTGCAACTT
TATCCGCCTC
CATCCAGTCT
ATTAATTGTT
GCCGGGAAGC
TAGAGTAAGT
AGTTCGCCAG
TTAATAGTTT
GCGCAACGTT
GTTGCCATTG
CTACAGGCAT
CGTGGTGTCA
CGCTCGTCGT
TTGGTATGGC
TTCATTCAGC
TCCGGTTCCC
AACGATCAAG
GCGAGTTACA
TGATCCCCCA
TGTTGTGCAA
AAAAGCGGTT
AGCTCCTTCG
GTCCTCCGAT
CGTTGTCAGA
AGTAAGTTGG
CCGCAGTGTT
ATCACTCATG
GTTATGGCAG
CACTGCATAA
TTCTCTTACT
GTCATGCCAT
CCGTAAGATG
CTTTTCTGTG
ACTGGTGAGT
ACTCAACCAA
GTCATTCTGA
GAATAGTGTA
TGCGGCGACC
GAGTTGCTCT
TGCCCGGCGT
CAATACGGGA
TAATACCGCG
CCACATAGCA
GAACTTTAAA
AGTGCTCATC
ATTGGAAAAC
GTTCTTCGGG
GCGAAAACTC
TCAAGGATCT
TACCGCTGTT
GAGATCCAGT
TCGATGTAAC
CCACTCGTGC
ACCCAACTGA
TCTTCAGCAT
CTTTTACTTT
CACCAGCGTT
TCTGGGTGAG
CAAAAACAGG
AAGGCAAAAT
GCCGCAAAAA
AGGGAATAAG
GGCGACACGG
AAATGTTGAA
TACTCATACT
CTTCCTTTTT
CAATATTATT
GAAGCATTTA
TCAGGGTTAT
TGTCTCATGA
GCGGATACAT
ATTTGAATGT
ATTTAGAAAA
ATAAACAAAT
AGGGGTTCCG
CGCACATTTC
CCCGAAAAGT
GCCACCTGAC
GTCGACGGAT
CGGGAGATCA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GATCAACTGG
ATAACTCAAG
CTAACCAAAA
TCATCCCAAA
CTTCCCACCC
CATACCCTAT
TACCACTGCC
AATTACCTGT
GGTTTCATTT
ACTCTAAACC
TGTGATTCCT
CTGAATTATT
TTCATTTTAA
AGAAATTGTA
TTTGTTAAAT
ATGTACTACA
AACTTAGTAG
T